Relevant bibliographies by topics / Periodontal tissue engineering

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Periodontal tissue engineering'

Author: Grafiati
Published: 11 December 2022
Last updated: 12 June 2025

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Journal articles on the topic "Periodontal tissue engineering"

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Iwata, Takanori, Masayuki Yamato, Isao Ishikawa, Tomohiro Ando, and Teruo Okano. "Tissue Engineering in Periodontal Tissue." Anatomical Record 297, no. 1 (2013): 16–25. http://dx.doi.org/10.1002/ar.22812.

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Carmagnola, Daniela, Gaia Pellegrini, Claudia Dellavia, Lia Rimondini, and Elena Varoni. "Tissue engineering in periodontology: Biological mediators for periodontal regeneration." International Journal of Artificial Organs 42, no. 5 (2019): 241–57. http://dx.doi.org/10.1177/0391398819828558.

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Teeth and the periodontal tissues represent a highly specialized functional system. When periodontal disease occurs, the periodontal complex, composed by alveolar bone, root cementum, periodontal ligament, and gingiva, can be lost. Periodontal regenerative medicine aims at recovering damaged periodontal tissues and their functions by different means, including the interaction of bioactive molecules, cells, and scaffolds. The application of growth factors, in particular, into periodontal defects has shown encouraging effects, driving the wound healing toward the full, multi-tissue periodontal regeneration, in a precise temporal and spatial order. The aim of the present comprehensive review is to update the state of the art concerning tissue engineering in periodontology, focusing on biological mediators and gene therapy.
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Ward, Emily. "A Review of Tissue Engineering for Periodontal Tissue Regeneration." Journal of Veterinary Dentistry 39, no. 1 (2021): 49–62. http://dx.doi.org/10.1177/08987564211065137.

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Periodontal disease is one of the most common diagnoses in small animal veterinary medicine. This infectious disease of the periodontium is characterized by the inflammation and destruction of the supporting structures of teeth, including periodontal ligament, cementum, and alveolar bone. Traditional periodontal repair techniques make use of open flap debridement, application of graft materials, and membranes to prevent epithelial downgrowth and formation of a long junctional epithelium, which inhibits regeneration and true healing. These techniques have variable efficacy and are made more challenging in veterinary patients due to the cost of treatment for clients, need for anesthesia for surgery and reevaluation, and difficulty in performing necessary diligent home care to maintain oral health. Tissue engineering focuses on methods to regenerate the periodontal apparatus and not simply to repair the tissue, with the possibility of restoring normal physiological functions and health to a previously diseased site. This paper examines tissue engineering applications in periodontal disease by discussing experimental studies that focus on dogs and other animal species where it could potentially be applied in veterinary medicine. The main areas of focus of tissue engineering are discussed, including scaffolds, signaling molecules, stem cells, and gene therapy. To date, although outcomes can still be unpredictable, tissue engineering has been proven to successfully regenerate lost periodontal tissues and this new possibility for treating veterinary patients is discussed.
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Dentino, A. "Tissue Engineering for Periodontal Regeneration." Yearbook of Dentistry 2006 (January 2006): 87. http://dx.doi.org/10.1016/s0084-3717(08)70078-3.

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Kim, Min Guk, and Chan Ho Park. "Tooth-Supporting Hard Tissue Regeneration Using Biopolymeric Material Fabrication Strategies." Molecules 25, no. 20 (2020): 4802. http://dx.doi.org/10.3390/molecules25204802.

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The mineralized tissues (alveolar bone and cementum) are the major components of periodontal tissues and play a critical role to anchor periodontal ligament (PDL) to tooth-root surfaces. The integrated multiple tissues could generate biological or physiological responses to transmitted biomechanical forces by mastication or occlusion. However, due to periodontitis or traumatic injuries, affect destruction or progressive damage of periodontal hard tissues including PDL could be affected and consequently lead to tooth loss. Conventional tissue engineering approaches have been developed to regenerate or repair periodontium but, engineered periodontal tissue formation is still challenging because there are still limitations to control spatial compartmentalization for individual tissues and provide optimal 3D constructs for tooth-supporting tissue regeneration and maturation. Here, we present the recently developed strategies to induce osteogenesis and cementogenesis by the fabrication of 3D architectures or the chemical modifications of biopolymeric materials. These techniques in tooth-supporting hard tissue engineering are highly promising to promote the periodontal regeneration and advance the interfacial tissue formation for tissue integrations of PDL fibrous connective tissue bundles (alveolar bone-to-PDL or PDL-to-cementum) for functioning restorations of the periodontal complex.
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Zhao, Ming, Qiming Jin, Janice E. Berry, Francisco H. Nociti, William V. Giannobile, and Martha J. Somerman. "Cementoblast Delivery for Periodontal Tissue Engineering." Journal of Periodontology 75, no. 1 (2004): 154–61. http://dx.doi.org/10.1902/jop.2004.75.1.154.

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Reichert da Silva Assunção, Luciana, Renato Colenci, Caril Constante Ferreira do-Amaral, et al. "Periodontal Tissue Engineering After Tooth Replantation." Journal of Periodontology 82, no. 5 (2011): 758–66. http://dx.doi.org/10.1902/jop.2010.100448.

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Izumi, Yuichi, Akira Aoki, Yoichi Yamada, et al. "Current and future periodontal tissue engineering." Periodontology 2000 56, no. 1 (2011): 166–87. http://dx.doi.org/10.1111/j.1600-0757.2010.00366.x.

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Maeda, Hidefumi, Shinsuke Fujii, Atsushi Tomokiyo, Naohisa Wada, and Akifumi Akamine. "Periodontal Tissue Engineering: Defining the Triad." International Journal of Oral & Maxillofacial Implants 28, no. 6 (2013): e461-e471. http://dx.doi.org/10.11607/jomi.te26.

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Ivanovski, S., C. Vaquette, S. Gronthos, D. W. Hutmacher, and P. M. Bartold. "Multiphasic Scaffolds for Periodontal Tissue Engineering." Journal of Dental Research 93, no. 12 (2014): 1212–21. http://dx.doi.org/10.1177/0022034514544301.

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Dissertations / Theses on the topic "Periodontal tissue engineering"

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Alotaibi, Dalal. "Aligned polymer scaffolds in periodontal tissue engineering." Thesis, University of Sheffield, 2014. http://etheses.whiterose.ac.uk/6260/.

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Periodontal disease is characterised by progressive gingival inflammation and degradation of the periodontal ligament (PDL) and alveolar bone. Recently, a limited number of studies have started to consider the use of tissue engineering approaches to facilitate periodontal tissue regeneration. Within the wider field of the skeletal bioengineering, research has been directed towards fabrication of aligned-fibre scaffolds and devices for reconstruction of larger ligaments and tendons for use in orthopaedic indications. Mechanical loading and growth factors are also known to influence the quality of engineered load-bearing musculoskeletal tissues; and it is increasingly being acknowledged that appropriate biomechanical cues are essential for appropriate organisation of the extracellular matrix (ECM). The aims of this study were to evalute the effect of fibre-alignment on cell behaviour and investigate the effect of either mechanical loads or growth factors on the quality of the resultant tissue engineered PDL tissue. Synthetic and natural scaffolds were prepared in aligned and random-fibre forms, and human periodontal ligament fibroblasts (HPDLFs) were cultured on these scaffolds and their biological responses were investigated. In aligned-fibre constructs, histochemical and immunochemical staining showed that HPDLFs were elongated in shape and oriented along the long-axis of the fibres and showed evidence of increased ECM deposition. Gene expression data showed that HPDLFs on aligned-fibre scaffolds expressed a more ligament-like phenotype, indicated by an increased expression of collagen type I (COL1A1) and periostin (POSTN) genes over the 20 days culture period. The results showed that static mechanical strain up-regulated the ligamentous genes namely; collagen type I, periostin and scleraxis (SCXA) with greater expression observed in aligned-fibre constructs. These effects were more marked in the aligned-fibre scaffolds. In contrast, Emdogain® (EMD) was found to promote the osteoblastic phenotype of HPDLFs as indicated by the up-regulation of alkaline phosphatase (ALPL) gene expression in the engineered tissue, while transforming growth factor beta 1 (TGF-β1) had more effect on the ligamentous genes (COL1A1, POSTN). This effect of EMD was also potentiated by the fibre-alignment of the scaffolds. EMD and TGF-β1 were observed to have a limited effect on HPDLF proliferation in the aligned-fibre constructs by day 14 of incubation regardless of whether EMD and TGF-β1 were added alone or in combination with each other. Although the exact mechanism by which EMD and TGF-β1 affected cell behaviour is unknown, the data suggested that their effects were heavily dependent on the cell phenotype and stage of differentiation which, in turn was greatly influenced by the alignment of the scaffold fibres. In conclusion, 3D tissue engineered PDL constructs, with good biological quality, can be developed using aligned-fibre scaffolds. These constructs have great potential for us as an in vitro model to study PDL regeneration and repair processes and ultimately, may inform research directed at new clinical applications.
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PIVA, PAOLO. "Tissue engineering in oro-maxillary bone regeneration." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2013. http://hdl.handle.net/2108/203399.

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To synthesize a polyurethane (PU) foam-like scaffold which could perform both as a resorbable membrane and as a cavity filling material in oro-maxillary bone defects. MATERIALS & METHODS: A PU foam was synthesized via a onepot reaction starting from a pre-polymerized isocyanate and a biocompatible polyester diol, using water as a foaming agent. Different foaming conditions were examined, with the aim of creating a dense/porous functional graded material. The obtained PU was characterized in terms of morphological and mechanical properties. Biocompatibility assessment was performed in combination with bonemarrow- derived human mesenchymal stromal cells (hBMSC). RESULTS: PU showed a highly porous structure, consisting of interconnected round pores with a diameter larger than 200 μm. Degradation test showed a slow degradation (ca. 1% weight loss after 6 weeks). Mechanical properties were strongly dependent upon foaming conditions, and not significantly affected by in vitro degradation process. In vitro biocompatibility assessments combined with hBMSCs proved the materials non cytotoxic, with cell viability values higher than 95% after 24 hours. CONCLUSIONS: This work demonstrates the feasibility of fabricating biphasic dense/porous polyurethane foams by a confined foaming reaction. Results support the potential application of the synthesized materials in the treatment of oro-maxillary bone defects.
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Suaid, Fabricia Ferreira. "Avaliação histométrica do efeito do transplante autógeno de células do ligamento periodontal no tratamento de defeitos de furca grau III em cães." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290846.

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Orientadores: Enilson Antônio Sallum, Karina Gonzales Silvério Ruiz<br>Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba<br>Made available in DSpace on 2018-08-16T00:46:02Z (GMT). No. of bitstreams: 1 Suaid_FabriciaFerreira_D.pdf: 4378938 bytes, checksum: 06a24b032e38b3ae490047a4a2ba7544 (MD5) Previous issue date: 2010<br>Resumo: O objetivo do presente estudo foi avaliar histometricamente o efeito dotransplante autógeno de células do ligamento periodontal (PDLCs), associado à regeneração tecidual guiada (RTG), no tratamento de defeitos de furca grau III criados cirurgicamente em cães. Inicialmente, as PDLCs foram obtidas das raízes do 2º pré-molar e do 1º molar inferior extraídos, bilateralmente, de sete cães da raça beagle. Em seguida, as células foram cultivadas in vitro e caracterizadas fenotipicamente. Lesões de furca grau III foram criadas nos 3os e 4os pré-molares inferiores e os defeitos foram aleatoriamente escolhidos para receber os seguintes tratamentos: Grupo Controle - instrumentação da superfície radicular com auxílio de curetas e posicionamento coronário dos retalhos (7); Grupo RTG - regeneração tecidual guiada (7); Grupo Esponja - RTG + esponja de colágeno (7); Grupo Células - RTG + células do ligamento periodontal embebidas na esponja de colágeno, na ausência de soro fetal bovino (7). Após três meses, os animais foram sacrificados e os blocos contendo os espécimes foram processados para análise histológica. Os parâmetros histométricos avaliados foram: extensão total do defeito (ETD), extensão não preenchida do defeito (ENP), novo cemento (NC), regeneração periodontal (RP), extensão de epitélio/conjuntivo (EEC), anquilose (ANQ), área total do defeito (ATD), área não preenchida (ANP), área preenchida (AP), área de novo osso (NO), área de epitélio/tecido conjuntivo (AEC). Resultados: A caracterização fenotípica, in vitro, demonstrou que as PDLCs foram capazes de promover a formação de nódulos minerais, bem como de expressar sialoproteína óssea (BSP), colágeno do tipo I (COL I) e a fosfatase alcalina (ALP). Histometricamente, a análise de dados demonstrou que o grupo tratado com células apresentou uma maior extensão de novo cemento (1,70 ± 0,60 mm; 2,87 ± 0,74 mm; 3,66 ± 0,95 mm e 4,82 ± 0,61mm, para os grupos controle, RTG, esponja e células, respectivamente; p<0,001), uma maior extensão da regeneração periodontal (0,69 ± 0,59 mm; 1,52 ± 0,39 mm; 2,33 ± 0,95 mm e 3,43 ± 1,44 mm, para os grupos controle, RTG, esponja e células, respectivamente; p = 0,001) e uma maior área de novo osso (1,89 ± 0,95 mm2; 2,91 ± 0,56 mm2; 3,94 ± 1,52 mm2 e 5,45 ± 1,58 mm2, para os grupos controle, RTG, esponja e células, respectivamente; p = 0, 0012). Dentro dos limites deste estudo, concluiu-se que o transplante autógeno de PDLCs associadas à RTG favoreceu a regeneração periodontal em defeitos de furca grau III.<br>Abstract: The aim of this study was to histometrically investigate the potential use of autogenous periodontal ligament cells (PDLCs) associated with guided tissue regeneration (GTR) for tissue engineering in surgically created class III furcation defects in dogs. PDLCs were obtained from the tooth root of bilateral mandibular 2nd premolar (P2) and the 1st molar (M1) extracted from seven beagle dogs, cultured in vitro and phenotypically characterized with regard to their biological properties. Bilateral class III furcation lesions were surgically created at 3rd and 4th premolars (P3, P4) and the defects were randomly assigned to one of the following groups: Control Group: root surface was scaled and planned with curettes and the flap was coronally positioned (n=7), GTR Group: two bioabsorbable membranes were adapted to cover the buccal and lingual aspects of the defect (n=7), Sponge Group: the collagen sponge scaffold was placed in the furcation area associated with GTR (n=7), Cell Group: the collagen sponge scaffold, with the cell suspension without FBS was placed in the furcation area associated with GTR (n=7). After 3 months, the animals were sacrificed and the blocks containing the experimental specimens were processed for histological analysis. The histometric parameters evaluated were: total defect length (TDL), tissue-free defect length (TFL), new cementum (NC), periodontal regeneration (R), epithelium/connective tissue extension (ECT), Ankylosis (ANQ), total defect area (TDA), non-filled area (NFA), soft tissue area (STA) and new bone area (NBA). Results: In vitro, phenotypic characterization demonstrated that PDLCs were able to promote mineral nodule formation as well as to express bone sialoprotein (BSP), type I collagen (COL I) and alkaline phosphatase (ALP). Histometrically, data analysis demonstrated that the cell-treated group presented a superior length of new cementum (1.70 ± 0.60 mm; 2.87 ± 0.74 mm; 3.66 ± 0.95 mm and 4.82 ± 0.61 mm, for control, GTR, sponge and cell groups, respectively; p<0.001), a greater extension of periodontal regeneration (0.69 ± 0.59 mm; 1.52 ± 0.39 mm; 2.33 ± 0.95 mm and 3.43 ± 1.44 mm, for control, GTR, sponge and cell groups, respectively; p=0.001) and a larger area of new bone (1.89 ± 0,95 mm2; 2.91 ± 0,56 mm2; 3.94 ± 1,52 mm2 and 5.45 ± 1,58 mm2, for control, GTR, sponge and cell groups, respectively; p=0,0012). Within the limits of this animal study, it was concluded that PDLCs in association with GTR may be a useful option to promote periodontal tissue regeneration in class III furcation defects.<br>Doutorado<br>Periodontia<br>Doutor em Clínica Odontológica
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Mondésert, Hugues. "Mineralization of PLGA nanofibers for periodontal tissue regeneration." Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/15298.

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Mestrado em Ciência e Engenharia de Materiais<br>Periodontal diseases induce a loss of soft and hard tissues surrounding the teeth after inflammation. Defects created by the infection would be replaced by the synthetic scaffold allowing progressive tissue regeneration. Mineralized PLGA (poly(lactic-­‐co-­‐glycolic acid)) nanofibers developed by electrospinningor jet spraying techniques are efficient biomaterials to maintain temporarily a physical structure and to enhance biocompatibility for hard tissue regeneration. The aim of this work was to mineralize PLGA nanofibers by two different methods: Simulated Body Fluid (SBF) immersion and projection by jet spraying (JS). SBF method consists in soaking PLGA matrices intohigh ions concentrated solutions (SBFx1 or SBFx5) to deposit mineral layers. With the new JS technique, we target a formation of a nanocomposite of PLGA and hydroxyapatite nanoparticles (nHA): first with the help of a blend solution (PLGA + nHA) directly projected (JS) and then with a simultaneous co projection of PLGA solution and nHA suspension in water (Co-­‐JS). From material characterization perspective, samples produced by SBFx1 protocol showed a very weak mineral deposition, low crystalline sodium chloride whereas SBFx5 solutions allowed the formation of a consequent CaP mineral layer on electrospun PLGA matrices. SEM images allowed the observation of different mineral structures strongly depending on SBF concentration and immersion time. XRD patterns confirmed the presence of HA into JS PLGA matrices. Morphologically, JS scaffolds varied with the concentration of HA nanoparticles incorporated into the initial mixture. HA nanoparticles were successfully incorporated inside the polymer fibers with the first Jet spraying technique (JS) whereas nHAs were successfully deposited on the surface of the PLGA fibers with Co JS method.<br>A doença periodontal induz uma inflamação que pode levar à destruição dos tecidos de suporte do dente. A degradação provocada pela doença pode ser tratada com o recurso a suportes sintéticos que permitam a regeneração progressiva dos tecidos. As nanofibras de ácido polilactico co-­‐glicolico (PLGA), mineralizadas, produzidas por electrofiação ou pela técnica de pulverização por jacto, são biomateriais adequados para funcionarem como suporte físico temporário e assegurarem a biocompatibilidade necessária à regeneração de tecidos. O presente trabalho tem como objetivo o estudo da mineralização de nano-­‐fibras de PLGA para optimizar a regeneração de tecidos duros. São propostos dois métodos de mineralização: o método baseado no fluido fisiológico simulado (SBF) e o método baseado na pulverização por jacto (JS). A técnica de SBF consiste em mergulhar matrizes de PLGA, produzidas por electrofiação, numa solução concentrada de sais ao passo que a técnica de JS consiste em pulverizar uma suspensão preparada com nanopartículas de hidroxiapatite (Ca5(PO4)3(OH), HA) e uma solução polimérica. Os materiais produzidos foram caracterizados por difração de Raios-­‐ X e por microscopia electrónica de varrimento (MEV).Para as amostras processadas pela técnica de SBF os resultados de DRX evidenciaram a presença de fosfatos de cálcio de baixa ristalinidade, correspondentes à fase de hidroxiapatite. As imagens de MEV permitiram observar a formação de estruturas minerais fortemente dependentes do tempo de imersão. Nas matrizes de PLGA tratadas por JS, a DRX confirmou a presença de HA e a MEV revelou que a morfologia das amostras depende da concentração das nanopartículas de HA adicionadas à solução polimérica inicial. O método de SBF permitiu uma deposição superficial de fosfatos de cálcio ao passo que, pelo método de JS, foi possível incorporar nanopartículas de HA no seio da matriz polimérica. A combinação dos dois métodos parece pois ser uma técnica promissora para fabricar suportes mineralizados para regeneração de tecido periodontal.
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Gay, Isabel C. "Isolation and characterization of human periodontal ligament stem cells." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. http://www.mhsl.uab.edu/dt/2007m/gay.pdf.

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Ochareon, Pannee. "Craniofacial periosteal cell capacities /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/6387.

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Martinez, Catalina. "The Effects of Dynamic Culturing Environments on Cell Populations Relevant to Heart Valve Tissue Engineering." FIU Digital Commons, 2011. http://digitalcommons.fiu.edu/etd/505.

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The design of a tissue engineered pulmonary valve (TEPV) involves cells source(s), scaffold, in vitro conditioning system and the functional stability of the TEPV in vivo. Vascular cells (pulmonary artery smooth muscle (SMCs) and endothelial cells (ECs)) and periodontal ligament derived stem cells (PDLSCs) are relevant sources for the designing of TEPVs. In this study, labeling of these cell populations with super paramagnetic iron oxide microparticles along with concomitant usage of transfection agents was followed by visualization using magnetic resonance, while Intracellular iron oxide was confirmed by prussian blue staining and fluorescence microscopy. Also, the potential of PDLSC as a feasible source for TEPVs was investigated, expressing differentiative capacity to both SMC and EC phenotypes by a combination of biochemical and mechanical stimulation. Flow conditioning in a u-shaped bioreactor augmented collagen production in SMC-EC (99.5% for n=3) and PDLSC (93.3% for n=3) seeded scaffolds after a 3-week culturing period (P<0.05).
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Han, Pingping. "Regulation of canonical Wnt signalling pathway during cementum regeneration." Thesis, Queensland University of Technology, 2014. https://eprints.qut.edu.au/75652/1/Pingping_Han_Thesis.pdf.

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This project highlights the important role of cell signalling pathway during tooth regeneration. Biomaterials can be designed to activate relevant cell signals for the purpose of dental repair and tooth regeneration. Based on the results in the present project, strategies directly targeting cell signalling pathway may provide new approaches for periodontal regenerative tissue engineering.
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Niada, S. "FROM IN VITRO STUDIES TO A LARGE ANIMAL MODEL: A MULTISTEP DISSECTION ON THE FUTURE ROLE OF ADIPOSE-DERIVED STEM CELLS FOR MUSCULOSKELETAL TISSUE ENGINEERING." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/229427.

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Tissue engineering is an emerging interdisciplinary field, born with the purpose to provide an alternative solution for the regeneration of lesioned or lost tissues, combining cells, biocompatible scaffolds and bioactive factors. The cells for this approach should be non-immunoreactive and non-tumorigenic. Moreover, they should be available in large amount and possess, or be able to acquire, a specific protein expression pattern similar to that of the damaged tissue and/or act as a pool of trophic factors for resident cells. All these reasons, make mesenchymal stem cells (MSCs) good candidates for applications in regenerative medicine. Although bone marrow is still the most common source of MSCs, these cells could be harvested from all vascularised tissues, and, interestingly, from tissues that are normally discarded, such as fat, placenta or umbilical cord. One of the most convenient source of MSCs, is unequivocally, the adipose tissue due to the easily accessible anatomical location and the abundance of subcutaneous adipose tissue. Adipose-derived stem cells (ASCs) are similar to MSCs isolated from bone marrow in morphology, immunophenotype, and differentiation ability, and own interesting features such as immunoregolatory and anti-inflammatory properties. In the recent years, many strategies for the cure of musculoskeletal tissues critical lesions, mainly in orthopaedic, oral and maxillo-facial surgery, have been under investigations. In this contest, the regeneration of structures including different tissues, such as the periodontium and the osteochondral unit, are particularly challenging. Periodontal regeneration is especially demanding, as it requires regeneration of three quite diverse and unique tissues such as the alveolar bone, the periodontal ligament and the cementum, that have to interface with each other to restore their complex structure. Since the promising results obtained with ASCs in preclinical studies of periodontal diseases arouse the curiosity of maxillofacial and dental surgeons, we decided to identify a novel source of ASCs, i.e, the buccal fat pad, convenient for these specialists. For this purpose, we studied human adipose derived-stem cells from buccal fat pad (BFP-ASCs), comparing them with cells from the subcutaneous adipose tissue (SC-ASCs) of the same donor (n=2). In parallel, considering the need for preclinical studies in which the effect of allogenic cells should be tested, and swine as an accepted animal model in tissue engineering applications, we also characterized porcine cells (n=6). With preclinical and clinical application prospective, we also investigated ASC interactions with oral tissues, natural and synthetic scaffolds and Amelogenin, an oral bioactive molecule. First of all, we showed that it is feasible to isolate ASCs even starting from very limited amounts of tissue (0,5 ml) and that the cellular yield is influenced by species, but not by the site of harvesting (1.1x105±1.4x104 human BFP-ASCs/ml and 1.15x105±7.1x103 human SC-ASCs/ml; 3.0x104±9.3x103 porcine BFP-ASCs/ml and 5.5x104±3.3x104 porcine SC-ASCs/ml). Despite the lower yield, the pASCs great proliferation rate allows to obtain high number of cells (potentially, 108 - 109) after few (3, 4) passages in culture. After the isolation, a great amount of cells deriving from all the tissues, adhered to cell culture plates showing the MSC fibroblast like morphology, with only mild shape differences constituted by the higher elongation and dimension of human SC-ASCs. Moreover, all the cells are easily expandable and showed good clonogenic ability at early passages. Cells of the same species, from both the harvesting site, displayed the same surface markers profile, that, in particular for human ASCs, was the typical one of hMSC (CD90+, CD105+, CD73+, CD14-, CD31-, and CD34-). Human and porcine BFP-ASCs, as SC-ASCs, are multipotent; indeed, when induced towards osteogenic and adipogenic lineages, they up-regulated significantly ALP activity, collagen and calcified extracellular matrix deposition and lipid vacuoles productions, respectively, already after 14 days of differentiation in vitro. Next, since cell/scaffold interaction is fundamental for the outcome of a tissue engineering approach, in sight of a preclinical study, we combined porcine BFP and SC-ASCs to both clinical grade (titanium) and innovative [silicon carbide–plasma-enhanced chemical vapor deposition (SIC-PECVD)] biomaterials, and studied cell adhesion and their differentiation ability. All the cells nicely grew on both scaffolds and, when osteoinduced, significantly increased the amount of calcified ECM compared to control cells; interestingly, titanium is osteoinductive even per se on pASCs (+284% and +91 for BFP- and SC-ASCs). Considering the importance of cell interaction with tissue of the lesion site, and with materials commonly used during surgical practices, we studied human BFP- and SC-ASC adherence to several supports. SEM analysis confirmed that both cell type nicely stick on alveolar bone, periodontal ligament, collagen membrane and polyglycolic acid filaments. Finally, we found that amelogenin, the most abundant enamel matrix protein seems to be an early osteoinductive factor for BFP-hASCs, whereas this effect is not manifested for SC-hASCs. For future cellular therapy, and since the use of FBS pose the risk of xenogenic contaminations leading to immunological complications during transplant, we tested cells growth in the presence of autologous supplements. Interestingly, both hASCs adapted rapidly to human serum, increasing their proliferation rates compared to standard culture condition, while porcine autologous or heterologous sera, did not improve pASC growth. In conclusion, we identified a cell population derived from a tissue easily available to dentists and maxillofacial surgeons, whose multipotent features and interaction with clinical grade scaffolds make proper candidate for future uses in tissue engineering approaches of periodontal diseases. In parallel, part of my PhD project was focused on the study of a critical osteochondral defect regeneration performed in a large animal preclinical model. The main obstacles for clinicians in treating this defect arises from the disparity concerning anatomy, composition and, most importantly, rate of healing of the articular cartilage (AC) and the subchondral bone. The key points of our study are the use of an innovative hydrogel of oligo(polyethylene glycol)fumarate (OPF) to fill the osteochondral defect, and of either porcine, or human ASCs, to create bioconstructs to be implanted in non-immuno-compromised minipigs. In particular, four critical osteochondral defects (diameter 9mm, depth 8mm) were created in the peripheral part of the trochlea of seven animals (defect n=28), and then treated with the different pre-made constructs. Untreated defects and defects filled by just scaffold were included as controls. No side-effects have been observed during the six-moths follow-up. At the end of this period, animals were sacrificed and knees explanted. Gross appearance analyses showed quite satisfactory filling of all the lesions, with the exception of one animal, whose joint appeared infected and not healed. MRI analyses revealed that in all the scaffold treated groups an overall improvement of the tissue quality at the osteochondral lesion site, was induced. More accurate evaluations (histological and immunohistochemistry analyses) revealed that some important tissue features were significantly improved by the association of OPF and ASCs. Indeed, regarding the subchondral bone, in all the OPF+ASCs groups, a mature bone appeared, with higher deposition of collagen type I compared to untreated or unseeded OPF groups. Moreover, the use of ASCs associated to scaffolds induced an improvement in newly formed cartilage features such as collagen type II deposition, and histological scores associated to these samples indicated a significant increase in matrix staining, tissue morphology and formation of tidemark, together with a reduction in vascularisation (a positive aspect in cartilage) compared to unseeded scaffolds. However, the histology indicated that in all the samples cartilage regeneration was still immature, most likely due to the limited time of follow up and/or the insufficient stimuli for cartilage complete regeneration. Despite this, biomechanical tests revealed that the neo-cartilage found in the cell-loaded scaffold groups possessed poroelastic behaviour, as well as indentation modulus and creep curves comparable to native cartilage. This important result suggest that the ASC presence at the lesion site, is able to enhance newly formed cartilage functionality. In conclusion, this in vivo study provide the evidence that both porcine and human adipose-derived stem cells associated to OPF hydrogel improve osteochondral defect regeneration, even though, at the moment, we are not able to define if the implanted ASCs are responsible per se of the new tissue formation or if they help spontaneous regeneration process by paracrine actions.
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Vernon, Lauren Louise. "A Comparison of the Osteogenic Tissue Engineering Potential of Dental-Derived Stem Cell Lines: Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs) vs. Periodontal Ligament Stem Cells (PERIOS)." Scholarly Repository, 2010. http://scholarlyrepository.miami.edu/oa_theses/19.

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The goal of this study is to assess the osteogenic potential of two types of dental stem cell lines within a tissue engineering application. More specifically, the goal of this study is to find a readily abundant cell source with capacity to express an osteogenic phenotype. There are two parameters utilized to evaluate tissue engineering potential of cells: proliferation rate and differentiation potential. Briefly, proliferation rate is the speed at which cells divide and differentiation potential determines if cells are capable of committing towards specific lineages (e.g. osteogenic). These components are important, because if cells are not expanding at a specific rate and are not differentiating towards the lineage desired, the tissue engineered will not mirror the characteristics of native tissue. Therefore, both components are necessary for osteogenic tissue engineering applications. Several stem cell lines have been isolated from different sources (e.g. umbilical, bone marrow) and characterized for their proliferative capacity and their potency. Among these progenitor or stem cell lines, are those isolated from human dental tissue. Due to the similarities between teeth and bone, this specific cell line may be useful in osteogenic tissue engineering applications. In this study, stem cells extracted from human exfoliated deciduous teeth (SHEDs) and periodontal ligament stem cells (PERIOs), were evaluated and compared. Briefly, to evaluate the proliferation rate an ex-vivo expansion study was conducted. This experiment found that both SHEDs and PERIOs were proliferative lines with doubling times of 23 hours and 19 hours respectively. Subsequently, osteogenic differentiation of SHEDs and PERIOs was assessed utilizing a 3-D fibrin gel suspension treated with osteogenic media containing either dexamethasone (DEX) or Retinoic Acid (RA) for 28 days. At day 28, osteogenic markers for collagen 1 (Col1), osteocalcin (OCN), and alkaline phosphatase (ALP) were evaluated using qPCR. Results demonstrated both SHEDs and PERIOs exhibited significant (p<0.05) increases in osteogenic gene expression under the influences of DEX and RA. However the most significant increases were expressed by the SHEDs that received the DEX treatment. Additionally, the synergistic ability of TGF-beta 3 on the osteogenic differentiation of the stem cells was evaluated. Cells were cultured in a 3-D fibrin gel suspension and allowed to differentiate in DEX osteogenic media with and without the supplementation of TGF-beta 3 for 21 days. Using qPCR the cells were evaluated for expression of Col1, OCN, and ALP. In both the SHEDs and PERIOs, the samples treated with TGF-beta 3 the osteogenic gene expression increased in reference to the control, but had a hindering effect compared to cells treated in DEX without the TGF-beta 3. These results from this study suggested, SHED cells grown in 3-D fibrin gel suspension, may be better than PERIO cells for osteogenic tissue engineering applications when treated with DEX media without the supplementation of TGF-beta 3.
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Books on the topic "Periodontal tissue engineering"

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Regenerative dentistry. Morgan & Claypool, 2010.

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Regenerative Endodontics, an Issue of Dental Clinics. Elsevier - Health Sciences Division, 2012.

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Book chapters on the topic "Periodontal tissue engineering"

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Ivanovski, Saso, P. Mark Bartold, Stan Gronthos, and Dietmar W. Hutmacher. "Periodontal tissue engineering." In Tissue Engineering and Regeneration in Dentistry. John Wiley & Sons, Ltd, 2016. http://dx.doi.org/10.1002/9781119282181.ch7.

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Ripamonti, Ugo, Jean-Claude Petit, and June Teare. "Tissue Engineering of the Periodontal Tissues." In Regenerative Dentistry. Springer International Publishing, 2010. http://dx.doi.org/10.1007/978-3-031-02581-5_3.

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Iranparvar, Aysel, Amin Nozariasbmarz, Sara DeGrave, and Lobat Tayebi. "Tissue Engineering in Periodontal Regeneration." In Applications of Biomedical Engineering in Dentistry. Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-21583-5_14.

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Rath, Avita, Preena Sidhu, Priyadarshini Hesarghatta Ramamurthy, Bennete Aloysius Fernandesv, Swapnil Shankargouda, and Sultan Orner Sheriff. "Gingiva and Periodontal Tissue Regeneration." In Current Advances in Oral and Craniofacial Tissue Engineering. CRC Press, 2020. http://dx.doi.org/10.1201/9780429423055-10.

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Diogenes, Anibal, Vanessa Chrepa, and Nikita B. Ruparel. "Clinical strategies for dental and periodontal disease management." In Tissue Engineering and Regeneration in Dentistry. John Wiley & Sons, Ltd, 2016. http://dx.doi.org/10.1002/9781119282181.ch8.

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Zhang, Bi, X. J. Zhang, C. Y. Bao, et al. "Repairing Periodontal Bone Defect with In Vivo Tissue Engineering Bone." In Key Engineering Materials. Trans Tech Publications Ltd., 2007. http://dx.doi.org/10.4028/0-87849-422-7.1121.

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Gimbel, Craig B. "Optical Coherence Tomography Imaging for Evaluating the Photobiomodulation Effects on Tissue Regeneration in Periodontal Tissue." In Lecture Notes in Electrical Engineering. Springer US, 2008. http://dx.doi.org/10.1007/978-0-387-71809-5_16.

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Menicanin, Danijela, K. Hynes, J. Han, S. Gronthos, and P. M. Bartold. "Cementum and Periodontal Ligament Regeneration." In Engineering Mineralized and Load Bearing Tissues. Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-22345-2_12.

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Al-Habib, Mey, and George T. J. Huang. "Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, and Primary Teeth Stem Cells—Isolation, Characterization, and Expansion for Tissue Engineering." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9012-2_7.

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Chen, Fa-Ming, and Songtao Shi. "Periodontal Tissue Engineering." In Principles of Tissue Engineering. Elsevier, 2014. http://dx.doi.org/10.1016/b978-0-12-398358-9.00072-0.

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Conference papers on the topic "Periodontal tissue engineering"

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Peng, Lin, and Ren-Xi Zhuo. "Biological Evaluation of Porous Chitosan/collagen Scaffolds for Periodontal Tissue Engineering." In 2008 2nd International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2008. http://dx.doi.org/10.1109/icbbe.2008.220.

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Demidov, Andrey V., Ekaterina V. Udaltsova, and Sergey M. Gerashchenko. "Development of the System for Assessment of Periodontal Tissue State." In 2021 IEEE Ural Symposium on Biomedical Engineering, Radioelectronics and Information Technology (USBEREIT). IEEE, 2021. http://dx.doi.org/10.1109/usbereit51232.2021.9455109.

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Sayed, Ahmed, Ahmed Mahmoud, Eros Chaves, Richard Crout, Kevin Sivaneri, and Osama Mukdadi. "Assessment of Gingival Inflammation Using Ultrasound Imaging." In ASME 2012 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/imece2012-89627.

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Gingivitis is the most common gingival inflammation in the oral cavity, and the most prevalent periodontal disease affecting 90% of the population in all age groups. Recently, a few research groups have investigated the possibility of using ultrasound in dentistry, particularly in diagnosing bony destruction in the more severe form of periodontal disease called periodontitis. This work investigates the feasibility of using ultrasound imaging to quantitatively assess gingival tissue inflammation. Signal and image processing of ultrasound data have been performed to quantitatively assess gingival tissue. A number of gingival scans were conducted in vitro to render ultrasound images of high-spatial and contrast resolutions. For each sample the B-mode images were matched with almost the same slices in histology. Results show that ultrasound scans for tissues with gingivitis exhibited low intensity of reflections (hypo echoic) at the inflamed tissues, while healthy dense epithelium layers exhibited higher reflections (hyper echoic). Histological diagnosis revealed good agreement with the ultrasound results indicating the usefulness of such ultrasound imaging in diagnosing gingivitis. In addition, a new design for an intraoral linear array ultrasound probe is demonstrated and utilized in our clinic in vivo. Analysis of the echogenicity patterns of the resultant images demonstrates the potential of using such a new probe in gingival health assessment, which would be feasible and clinical relevant for patient evaluations clinically.
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Qasim, S. B., R. Delaine-Smith, A. Rawlinson, and I. U. Rehman. "Development of a Novel Bioactive Functionally Guided Tissue Graded Membrane for Periodontal Lesions." In University of Sheffield Engineering Symposium. USES, 2015. http://dx.doi.org/10.15445/01012014.07.

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Li, Ze-jian, Chun-ting Lu, Shu-yuan Ma, Ren-fa Lai, and Jiong Li. "Cultivation of periodontal tissue cell sheet by a new way for cell sheet engineering." In 2016 Sixth International Conference on Information Science and Technology (ICIST). IEEE, 2016. http://dx.doi.org/10.1109/icist.2016.7483376.

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Yang, Yu, Wencheng Tang, and Yao jun Wang. "Experimental Analysis of the Elastic Modulus of Periodontal Ligament in Nanoindentation." In ASME 2016 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2016. http://dx.doi.org/10.1115/detc2016-59040.

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The periodontal ligament (PDL) is a soft connective tissue which exhibits an inhomogeneous, nonlinear, and anisotropic material properties. and the elastic modulus of different positions on each section are not the same, analysis of the material properties of PDL enables a better understanding of biomechanical features for tooth movement. The aim of this study was to study the elastic modulus of different section of PDL in nanoindentation. Experimental results indicate that the average elastic modulus elastic modulus in midroot are lower than cervical margin and apex, and there is large change in the circumferential regions.
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Lin, Ting-Ju, Yen-Hong Lin, Yi-Wen Chen, and Ming-You Shie. "The Effect of Tensile Force and Periodontal Ligament Cell-Laden Calcium Silicate/Bioinks Auxetic Scaffolds for Tissue Engineering." In 2022 IEEE 22nd International Conference on Bioinformatics and Bioengineering (BIBE). IEEE, 2022. http://dx.doi.org/10.1109/bibe55377.2022.00032.

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Natali, Arturo N., Emanuele L. Carniel, Piero G. Pavan, et al. "Constitutive Formulation for Numerical Analysis of Visco-Hyperelastic Damage Phenomena in Soft Biological Tissues." In ASME 8th Biennial Conference on Engineering Systems Design and Analysis. ASMEDC, 2006. http://dx.doi.org/10.1115/esda2006-95254.

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Soft biological tissues show a strongly non linear and time-dependent mechanical response and undergo large strains under physiological loads. The microstructural arrangement determines specific anisotropic macroscopic properties that must be considered within a constitutive formulation. The characterization of the mechanical behaviour of soft tissues entails the definition of constitutive models capable of accounting for geometric and material non linearity. In the model presented here a hyperelastic anisotropic formulation is adopted as the basis for the development of constitutive models for soft tissues and can be properly arranged for the investigation of viscous and damage phenomena as well to interpret significant aspects pertaining to ordinary and degenerative conditions. Visco-hyperelastic models are used to analyze the time-dependent mechanical response, while elasto-damage models account for the stiffness and strength decrease that can develop under significant loading or degenerative conditions. Experimental testing points out that damage response is affected by the strain rate associated with loading, showing a decrease in the damage limits as the strain rate increases. This phenomena can be investigated by means of a model capable of accounting for damage phenomena in relation to viscous effects. The visco-hyperelastic damage model developed is defined on the basis of a Helmholtz free energy function depending on the strain-damage history. In particular, a specific damage criterion is formulated in order to evaluate the influence of the strain rate on damage. The model can be implemented in a general purpose finite element code. This makes it possible to perform numerical analyses of the mechanical response considering time-dependent effects and damage phenomena. The experimental tests develop investigated tissue response for different strain rate conditions, accounting for stretch situations capable of inducing damage phenomena. The reliability of the formulation is evaluated by a comparison with the results of experimental tests performed on pig periodontal ligament.
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WANG, Lan-lei, He-ying HOU, Sheng-yan YU, Ai-zhong GUAN, and Yun-mao LIAO. "The Histological Study of Orthodontic Force on the Periodontal Tissues Regenerated by Nano Bioceramics in Beagle Dogs." In 2nd International Conference on Biomedical and Biological Engineering 2017 (BBE 2017). Atlantis Press, 2017. http://dx.doi.org/10.2991/bbe-17.2017.60.

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Strusovskaya, A., S. Poroysky, A. Smirnov, et al. "A study of the influence of barbaris root (Berberis vulgaris L., Berberidaceae) extract dental gel on the dynamics of the inflammatory process in periodontal tissues of rats on the model of induced gingivitis." In PROCEEDINGS OF INTERNATIONAL CONFERENCE ON RECENT TRENDS IN MECHANICAL AND MATERIALS ENGINEERING: ICRTMME 2019. AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0019185.

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Reports on the topic "Periodontal tissue engineering"

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Zhang, Yuhao, Wenheng Zhao, Liyang Jia, Nan Xu, Yan Xiao, and Qiyan Li. The application of stem cells in tissue engineering for periodontal defects in randomized controlled trial: a systematic review and meta-analysis. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, 2022. http://dx.doi.org/10.37766/inplasy2022.1.0036.

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