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1
Pereira, Sergio Luis da Silva. "Avaliação histologica e histometrica do uso de membramas não reabsorviveis e reabsorviveis em defeitos periodontais cirurgicamente criados em cães." [s.n.], 1999. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290833.
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Orientadores: Enilson Antonio Sallum, Antonio Wilson SallumTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: O objetivo deste trabalho foi comparar, histológica e histometricamente, o processo de cura de defeitos tipo deiscência tratados pela técnica de regeneração tecidual guiada (RTG) com membranas reabsorvíveis de ácido poliláctico e não reabsorvíveis de politetrafluoroetileno expandido (PTFE-e). Seis cães adultos fêmeas de raça indefinida foram utilizados. Defeitos ósseos tipo deiscência foram criados cirurgicamente nas raízes distais dos terceiros e quartos pré molares mandibulares de ambos os lados e expostos ao acúmulo de placa por 3 meses. Após este período, os defeitos foram aleatoriamente designados para um dos tratamentos: RTG com membrana reabsorvível de ácido poliláctico (Grupo 1), RTG com membrana não reabsorvível de PTFE-e (Grupo 2), raspagem e alisamento radicular manual com acesso cirúrgico (Grupo 3) e não tratado (Grupo 4). Após 3 meses do segundo procedimento cirúrgico, os cães foram sacrificados e os espécimes processados para permitir análise histológica e histométrica, incluindo. os seguintes parâmetros: extensão linear do epitélio sulcular e juncional, adaptação do tecido conjuntivo, novo cemento, extensão vertical do novo osso e nova área óssea. Uma extensão linear de novo cemento estatisticamente superior (P<0.05) foi observada nos sítios tratados pela RTG, independente do tipo de membrana utilizada, em comparação com o Grupo 3. Não houve diferença estatisticamente significante entre o Grupo 1 e 2 em todos os parâmetros avaliados, exceto em relação à área de novo osso. O grupo 1 apresentou uma área de novo osso estatisticamente superior a dos outros grupos (P<0.05). Dentro dos limites deste estudo pôde-se concluir que ambas as membranas foram igualmente efetivas em promover nova formação cementária e que a membrana reabsorvível de ácido poliláctico (não-suturada) providenciou uma maior área óssea em relação à membrana não reabsorvível de PTFE-e
Abstract: The goal of this investigation was to compare histollogically and histometrically the healing process of dehiscence-type defects treated by guided tissue regeneration (GTR) with resorbable polylactic acid membranes and nonresorbable ePTFE membranes. Six mongrel dogs were used. Buccal osseous dehiscences were surgically created on the distal roots of the mandibular third and fourth premolars. The defects were exposed to plaque accumulation for 3 months. After this period, the defects were randomly assigned to one ofthe treatments: GTR with resorbable membrane (GTR1), GTR with nonresorbable membrane (GTR2), open flap debridement (OFD) and non-treated control (NTC). After 3 months of healing, the dogs were sacrificed and the blocks were processed. The histometric parameters evaluated included: length of sulcular and junctional epithelium, connective tissue adaptation, new cementum, new bone (vertical component) and new bone area. A superior length of new cementum was observed in the sites treated by GTR, regardless of the type of barrier used (P<0.05), in comparison with OFD. No statistically significant differences were found between GTRl and GTR2 in all the parameters with the exception ofbone area. GTRl presented a greater bone area (P<0.05) when compared to GTR2, OFD and NTC. Within the limits of this study, it can be concluded that both batriers are equally effective for new cementum formation. The resorbable membrane (non-sutured) may provide a better osseous response than the nonresorbable membrane
Doutorado
Periodontia
Doutor em Clínica Odontológica
2
Alotaibi, Dalal. "Aligned polymer scaffolds in periodontal tissue engineering." Thesis, University of Sheffield, 2014. http://etheses.whiterose.ac.uk/6260/.
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Periodontal disease is characterised by progressive gingival inflammation and degradation of the periodontal ligament (PDL) and alveolar bone. Recently, a limited number of studies have started to consider the use of tissue engineering approaches to facilitate periodontal tissue regeneration. Within the wider field of the skeletal bioengineering, research has been directed towards fabrication of aligned-fibre scaffolds and devices for reconstruction of larger ligaments and tendons for use in orthopaedic indications. Mechanical loading and growth factors are also known to influence the quality of engineered load-bearing musculoskeletal tissues; and it is increasingly being acknowledged that appropriate biomechanical cues are essential for appropriate organisation of the extracellular matrix (ECM). The aims of this study were to evalute the effect of fibre-alignment on cell behaviour and investigate the effect of either mechanical loads or growth factors on the quality of the resultant tissue engineered PDL tissue. Synthetic and natural scaffolds were prepared in aligned and random-fibre forms, and human periodontal ligament fibroblasts (HPDLFs) were cultured on these scaffolds and their biological responses were investigated. In aligned-fibre constructs, histochemical and immunochemical staining showed that HPDLFs were elongated in shape and oriented along the long-axis of the fibres and showed evidence of increased ECM deposition. Gene expression data showed that HPDLFs on aligned-fibre scaffolds expressed a more ligament-like phenotype, indicated by an increased expression of collagen type I (COL1A1) and periostin (POSTN) genes over the 20 days culture period. The results showed that static mechanical strain up-regulated the ligamentous genes namely; collagen type I, periostin and scleraxis (SCXA) with greater expression observed in aligned-fibre constructs. These effects were more marked in the aligned-fibre scaffolds. In contrast, Emdogain® (EMD) was found to promote the osteoblastic phenotype of HPDLFs as indicated by the up-regulation of alkaline phosphatase (ALPL) gene expression in the engineered tissue, while transforming growth factor beta 1 (TGF-β1) had more effect on the ligamentous genes (COL1A1, POSTN). This effect of EMD was also potentiated by the fibre-alignment of the scaffolds. EMD and TGF-β1 were observed to have a limited effect on HPDLF proliferation in the aligned-fibre constructs by day 14 of incubation regardless of whether EMD and TGF-β1 were added alone or in combination with each other. Although the exact mechanism by which EMD and TGF-β1 affected cell behaviour is unknown, the data suggested that their effects were heavily dependent on the cell phenotype and stage of differentiation which, in turn was greatly influenced by the alignment of the scaffold fibres. In conclusion, 3D tissue engineered PDL constructs, with good biological quality, can be developed using aligned-fibre scaffolds. These constructs have great potential for us as an in vitro model to study PDL regeneration and repair processes and ultimately, may inform research directed at new clinical applications.
3
Mondésert, Hugues. "Mineralization of PLGA nanofibers for periodontal tissue regeneration." Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/15298.
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Mestrado em Ciência e Engenharia de MateriaisPeriodontal diseases induce a loss of soft and hard tissues surrounding the teeth after inflammation. Defects created by the infection would be replaced by the synthetic scaffold allowing progressive tissue regeneration. Mineralized PLGA (poly(lactic-‐co-‐glycolic acid)) nanofibers developed by electrospinningor jet spraying techniques are efficient biomaterials to maintain temporarily a physical structure and to enhance biocompatibility for hard tissue regeneration. The aim of this work was to mineralize PLGA nanofibers by two different methods: Simulated Body Fluid (SBF) immersion and projection by jet spraying (JS). SBF method consists in soaking PLGA matrices intohigh ions concentrated solutions (SBFx1 or SBFx5) to deposit mineral layers. With the new JS technique, we target a formation of a nanocomposite of PLGA and hydroxyapatite nanoparticles (nHA): first with the help of a blend solution (PLGA + nHA) directly projected (JS) and then with a simultaneous co projection of PLGA solution and nHA suspension in water (Co-‐JS). From material characterization perspective, samples produced by SBFx1 protocol showed a very weak mineral deposition, low crystalline sodium chloride whereas SBFx5 solutions allowed the formation of a consequent CaP mineral layer on electrospun PLGA matrices. SEM images allowed the observation of different mineral structures strongly depending on SBF concentration and immersion time. XRD patterns confirmed the presence of HA into JS PLGA matrices. Morphologically, JS scaffolds varied with the concentration of HA nanoparticles incorporated into the initial mixture. HA nanoparticles were successfully incorporated inside the polymer fibers with the first Jet spraying technique (JS) whereas nHAs were successfully deposited on the surface of the PLGA fibers with Co JS method.
A doença periodontal induz uma inflamação que pode levar à destruição dos tecidos de suporte do dente. A degradação provocada pela doença pode ser tratada com o recurso a suportes sintéticos que permitam a regeneração progressiva dos tecidos. As nanofibras de ácido polilactico co-‐glicolico (PLGA), mineralizadas, produzidas por electrofiação ou pela técnica de pulverização por jacto, são biomateriais adequados para funcionarem como suporte físico temporário e assegurarem a biocompatibilidade necessária à regeneração de tecidos. O presente trabalho tem como objetivo o estudo da mineralização de nano-‐fibras de PLGA para optimizar a regeneração de tecidos duros. São propostos dois métodos de mineralização: o método baseado no fluido fisiológico simulado (SBF) e o método baseado na pulverização por jacto (JS). A técnica de SBF consiste em mergulhar matrizes de PLGA, produzidas por electrofiação, numa solução concentrada de sais ao passo que a técnica de JS consiste em pulverizar uma suspensão preparada com nanopartículas de hidroxiapatite (Ca5(PO4)3(OH), HA) e uma solução polimérica. Os materiais produzidos foram caracterizados por difração de Raios-‐ X e por microscopia electrónica de varrimento (MEV).Para as amostras processadas pela técnica de SBF os resultados de DRX evidenciaram a presença de fosfatos de cálcio de baixa ristalinidade, correspondentes à fase de hidroxiapatite. As imagens de MEV permitiram observar a formação de estruturas minerais fortemente dependentes do tempo de imersão. Nas matrizes de PLGA tratadas por JS, a DRX confirmou a presença de HA e a MEV revelou que a morfologia das amostras depende da concentração das nanopartículas de HA adicionadas à solução polimérica inicial. O método de SBF permitiu uma deposição superficial de fosfatos de cálcio ao passo que, pelo método de JS, foi possível incorporar nanopartículas de HA no seio da matriz polimérica. A combinação dos dois métodos parece pois ser uma técnica promissora para fabricar suportes mineralizados para regeneração de tecido periodontal.
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Gottlow, Jan. "New attachment formation by guided tissue regeneration." Göteborg : Dept. of Periodontology, University of Göteborg, 1986. http://catalog.hathitrust.org/api/volumes/oclc/17242123.html.
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Suaid, Fabricia Ferreira. "Avaliação histométrica do efeito do transplante autógeno de células do ligamento periodontal no tratamento de defeitos de furca grau III em cães." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290846.
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Orientadores: Enilson Antônio Sallum, Karina Gonzales Silvério RuizTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: O objetivo do presente estudo foi avaliar histometricamente o efeito dotransplante autógeno de células do ligamento periodontal (PDLCs), associado à regeneração tecidual guiada (RTG), no tratamento de defeitos de furca grau III criados cirurgicamente em cães. Inicialmente, as PDLCs foram obtidas das raízes do 2º pré-molar e do 1º molar inferior extraídos, bilateralmente, de sete cães da raça beagle. Em seguida, as células foram cultivadas in vitro e caracterizadas fenotipicamente. Lesões de furca grau III foram criadas nos 3os e 4os pré-molares inferiores e os defeitos foram aleatoriamente escolhidos para receber os seguintes tratamentos: Grupo Controle - instrumentação da superfície radicular com auxílio de curetas e posicionamento coronário dos retalhos (7); Grupo RTG - regeneração tecidual guiada (7); Grupo Esponja - RTG + esponja de colágeno (7); Grupo Células - RTG + células do ligamento periodontal embebidas na esponja de colágeno, na ausência de soro fetal bovino (7). Após três meses, os animais foram sacrificados e os blocos contendo os espécimes foram processados para análise histológica. Os parâmetros histométricos avaliados foram: extensão total do defeito (ETD), extensão não preenchida do defeito (ENP), novo cemento (NC), regeneração periodontal (RP), extensão de epitélio/conjuntivo (EEC), anquilose (ANQ), área total do defeito (ATD), área não preenchida (ANP), área preenchida (AP), área de novo osso (NO), área de epitélio/tecido conjuntivo (AEC). Resultados: A caracterização fenotípica, in vitro, demonstrou que as PDLCs foram capazes de promover a formação de nódulos minerais, bem como de expressar sialoproteína óssea (BSP), colágeno do tipo I (COL I) e a fosfatase alcalina (ALP). Histometricamente, a análise de dados demonstrou que o grupo tratado com células apresentou uma maior extensão de novo cemento (1,70 ± 0,60 mm; 2,87 ± 0,74 mm; 3,66 ± 0,95 mm e 4,82 ± 0,61mm, para os grupos controle, RTG, esponja e células, respectivamente; p<0,001), uma maior extensão da regeneração periodontal (0,69 ± 0,59 mm; 1,52 ± 0,39 mm; 2,33 ± 0,95 mm e 3,43 ± 1,44 mm, para os grupos controle, RTG, esponja e células, respectivamente; p = 0,001) e uma maior área de novo osso (1,89 ± 0,95 mm2; 2,91 ± 0,56 mm2; 3,94 ± 1,52 mm2 e 5,45 ± 1,58 mm2, para os grupos controle, RTG, esponja e células, respectivamente; p = 0, 0012). Dentro dos limites deste estudo, concluiu-se que o transplante autógeno de PDLCs associadas à RTG favoreceu a regeneração periodontal em defeitos de furca grau III.
Abstract: The aim of this study was to histometrically investigate the potential use of autogenous periodontal ligament cells (PDLCs) associated with guided tissue regeneration (GTR) for tissue engineering in surgically created class III furcation defects in dogs. PDLCs were obtained from the tooth root of bilateral mandibular 2nd premolar (P2) and the 1st molar (M1) extracted from seven beagle dogs, cultured in vitro and phenotypically characterized with regard to their biological properties. Bilateral class III furcation lesions were surgically created at 3rd and 4th premolars (P3, P4) and the defects were randomly assigned to one of the following groups: Control Group: root surface was scaled and planned with curettes and the flap was coronally positioned (n=7), GTR Group: two bioabsorbable membranes were adapted to cover the buccal and lingual aspects of the defect (n=7), Sponge Group: the collagen sponge scaffold was placed in the furcation area associated with GTR (n=7), Cell Group: the collagen sponge scaffold, with the cell suspension without FBS was placed in the furcation area associated with GTR (n=7). After 3 months, the animals were sacrificed and the blocks containing the experimental specimens were processed for histological analysis. The histometric parameters evaluated were: total defect length (TDL), tissue-free defect length (TFL), new cementum (NC), periodontal regeneration (R), epithelium/connective tissue extension (ECT), Ankylosis (ANQ), total defect area (TDA), non-filled area (NFA), soft tissue area (STA) and new bone area (NBA). Results: In vitro, phenotypic characterization demonstrated that PDLCs were able to promote mineral nodule formation as well as to express bone sialoprotein (BSP), type I collagen (COL I) and alkaline phosphatase (ALP). Histometrically, data analysis demonstrated that the cell-treated group presented a superior length of new cementum (1.70 ± 0.60 mm; 2.87 ± 0.74 mm; 3.66 ± 0.95 mm and 4.82 ± 0.61 mm, for control, GTR, sponge and cell groups, respectively; p<0.001), a greater extension of periodontal regeneration (0.69 ± 0.59 mm; 1.52 ± 0.39 mm; 2.33 ± 0.95 mm and 3.43 ± 1.44 mm, for control, GTR, sponge and cell groups, respectively; p=0.001) and a larger area of new bone (1.89 ± 0,95 mm2; 2.91 ± 0,56 mm2; 3.94 ± 1,52 mm2 and 5.45 ± 1,58 mm2, for control, GTR, sponge and cell groups, respectively; p=0,0012). Within the limits of this animal study, it was concluded that PDLCs in association with GTR may be a useful option to promote periodontal tissue regeneration in class III furcation defects.
Doutorado
Periodontia
Doutor em Clínica Odontológica
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Mayfield, Lisa. "Regeneration in periodontal and endosseous implant treatment." Malmö, Sweden : Dept. of Periodontology, Faculty of Odontology, Lund University, 1998. http://catalog.hathitrust.org/api/volumes/oclc/39457632.html.
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Borges, Ricardo Jorge Morais. "Regeneração periodontal de defeitos infra-ósseos." Master's thesis, [s.n.], 2015. http://hdl.handle.net/10284/5276.
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Projeto de Pós-Graduação/Dissertação apresentado à Universidade Fernando Pessoa como parte dos requisitos para obtenção do grau de Mestre em Medicina DentáriaA doença periodontal afeta grande parte da população, e a sua progressão pode levar à perda de inserção dos tecidos conetivos do periodonto assim como perda óssea. O tratamento periodontal procura essencialmente dois objetivos: impedir a progressão da doença e reconstruir os tecidos periodontais perdidos. O tratamento regenerativo surge como método para alcançar este segundo objetivo. Neste âmbito, ao longo do tempo têm sido desenvolvidas diversas técnicas regenerativas, sendo as proteínas de matriz de esmalte e a regeneração tecidular guiada as mais investigadas em ensaios clínicos. A revisão bibliográfica inicialmente foi realizada no motor de busca PubMed recorrendo a palavras-chave como: “Periodontal Regeneration”, “Intrabony Defects”, “Guided Tissue Regeneration” e “Enamel Matrix Proteins” tendo por base meta-análises publicadas, maioritariamente, nos últimos 10 anos. Posteriormente foram incluídos artigos como base litúrgica para abordar a parte teórica deste trabalho, sendo estes publicados entre 1958 e 2015.
Periodontal disease affects a large population and its progression may lead to the loss of attachment of periodontal connective tissue as well as bone loss. The periodontal treatment essentially seeks two objectives: preventing disease progression and rebuild the lost periodontal tissues. The regenerative treatment arises as a method to achieve this second goal. In this context, from time to time there have been developed several regenerative techniques, being the proteins of enamel matrix and guided tissue regeneration, the most investigated in clinical trials. The literature review was conducted initially in the search engine PubMed using keywords like "Periodontal Regeneration", "Intrabony Defects", "Guided Tissue Regeneration" and "Enamel Matrix Proteins" on the basis of published meta-analysis, mostly in the last 10 years. Later there were included items as a liturgical basis for in order to adress the theoretical part of this study, which were published between 1958 and 2015.
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França, Isabela Lima 1987. "Técnica de retalho semilunar posicionado coronariamente com ou sem associação à proteína derivada das matriz do esmalte para o tratamento de recessões gengivais : estudo clínico controlado randomizado." [s.n.], 2015. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290818.
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Orientador: Enilson Antonio SallumDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: O objetivo deste estudo foi avaliar, clinicamente, a utilização do Retalho Semilunar Posicionado Coronariamente (RSPC) para tratamento de recessões gengivais, com ou sem associação à proteína derivada da matriz do esmalte (EMD). Foram selecionados 30 pacientes, que foram randomizados e alocados em dois grupos: teste (RSPC + EMD) e controle (RSPC sozinho). Para serem incluídos no estudo, os indivíduos deveriam apresentar recessões gengivais vestibulares localizadas classe I de Miller com altura maior ou igual a 2,0mm e menor que 4,0 mm, em caninos ou pré-molares superiores. Parâmetros clínicos avaliados: altura da recessão gengival (ARG), largura da recessão gengival (LRG), nível de inserção clínica (NIC), profundidade de sondagem (PS), altura de tecido queratinizado (ATQ), espessura de tecido queratinizado (ETQ) e altura (AP) e largura (LP) das papilas mesial e distal, além de índice de placa (IPL) e índice gengival (IG). Estes parâmetros foram medidos nos seguintes períodos: baseline, 90 dias e 180 dias após o procedimento cirúrgico. Nenhuma diferença estatisticamente significante foi observada entre os grupos em relação à redução da retração gengival com 6 meses de acompanhamento, embora tenha sido encontrada maior porcentagem de cobertura radicular no grupo RSPC+EMD (91%), quando comparado ao RSPC (87%) (p>0,05). Cobertura radicular completa foi obtida em 60% dos sítios no RSPC enquanto no grupo RSPC+EMD foi observada em 66,67% dos sítios. Dentro dos limites do presente estudo pôde-se concluir que o RSPC, associado ou não a EMD, levou a redução da recessão gengival, sem diferença estatística entre os grupos, após 6 meses de acompanhamento pós-operatório
Abstract: The aim of this study was to evaluate clinically the use of the Semilunar Coronally Positioned Flap (SCPF) for the treatment of gingival recessions, with or without Enamel Matrix Derivative (EMD). Thirty patients were selected, randomized and allocated in two groups: test (SCPF + EMD) and control (SCPF alone). To attend the study, subjects should present buccal Miller class I gingival recessions with height greater than or equal to 2.0 mm and less than to 4.0 mm in maxillary canines or premolars. Clinical parameters evaluated: gingival recession height (GRH), gingival recession width (GRW), clinical attachment level (CAL), probing depth (PD), height (HKT) and thickness (TKT) of keratinized tissue and papillas height (HP) and width (LP), as well as plaque and gingival index. These data were collected at baseline, 90 days and 180 days after surgery. No statistically significant difference was observed between the groups regarding the reduction of gingival recession after 6 months of follow-up, although a higher percentage of root coverage was found in SCPF + EMD group (91%), when compared to the SCPF (87%) (p> 0.05). Complete root coverage was observed in 60% of the sites of the control group (SCPF alone) and in 66,67% of the sites of the test group (SCPF+EMD). Within the limits of this study it was concluded that SCPF, associated or not with EMD may provide a reduction in gingival recession, with no statistical difference between groups
Mestrado
Periodontia
Mestra em Clínica Odontológica
9
Hattingh, André Christiaan. "A protocol to study tissue regeneration in alveolar bony defects /." Access to E-Thesis, 1999. http://upetd.up.ac.za/thesis/available/etd-01052007-135643/.
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Qasim, Syed Saad B. "Development of novel functionally graded guided tissue regenerative membrane for periodontal lesions." Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/11219/.
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Periodontal diseases are chronic inflammatory conditions affecting the supporting tissues of teeth caused by the prolonged accumulation of micro-organisms in the biofilm that forms on tooth surfaces. Conventional non-surgical and surgical treatments aim to halt disease progression and repair the lost periodontal tissues. Surgical therapies such as open flap debridement are aimed to replace the lost alveolar bone and guided tissue regeneration (GTR) is also used to treat this disease. Whilst the regeneration of lost support is an aim of periodontal treatment, the outcomes of current approaches to periodontal regeneration (PR) are unpredictable. Consequently, significant laboratory and clinical research has been undertaken to explore the possibilities of improving the outcomes of treatment over the past few decades. In this respect chitosan (CH), a well-known biopolymer holds promise to be fabricated in various forms. The aim of the project was to fabricate a trilayered functionally graded GTR membrane by fabricating surface and core layers of non-porous and porous morphologies with complete physiochemical and biological characterisation. Solvent Casting, Freeze gelation (FG) and Electrospinning was performed on CH alone and in combination with Hydroxyapatite (HA). Membranes were characterized with Scanning electron Microscopy, Fourier Transform Infrared Spectroscopy (FTIR), tensile testing, long term degradation and swelling studies were also performed. Cell culturing was performed using human osteosarcoma and progenitor cell line. Sirius red and Alizarin red assays were conducted to assess matrix deposition. Amongst the non-porous membranes fabricated by solvent casting, with Low molecular weight (LMw) CH:HA ratio of 30:70 showed better biocompatibility, and amongst the porous membranes made up of FG, ASa (ascorbic acid) :CH:HA (50:50) showed better stability and biocompatibility after in-vitro analysis. Histology of FG membranes conducted after in-vivo studies showed ASa:CH:HA to have higher cellular infiltration after 30 days of implantation. Electrospun fibres obtained in both aligned and random orientations were conducive to cellular attachment and mineralized matrix deposition with time. FTIR analysis showed strong co-ordination bond formation in between CH and HA. HA incorporated samples treated with simulated body fluid (SBF) showed an embryonic layer formation of hydroxyl carbonated apatite. Membranes can be combined together in different ways to achieve structural and functionally graded structures. A template was prepared using solvent casting and freeze gelation techniques to achieve functional gradients. Furthermore; CH and HA composite membranes could possibly be used for GTR applications in periodontal lesions and in addition these techniques could be further tuned to achieve desirable characteristics of a GTR membrane for PR and also holds promise to be used in other biomedical applications.
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Gay, Isabel C. "Isolation and characterization of human periodontal ligament stem cells." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2007. http://www.mhsl.uab.edu/dt/2007m/gay.pdf.
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Lai, Man-lung Stanley. "Adjunctive effects of a low-power laser on the healing of periodontal tissue." Click to view the E-thesis via HKUTO, 2003. http://sunzi.lib.hku.hk/hkuto/record/B37651006.
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Lai, Man-lung Stanley, and 賴文龍. "Adjunctive effects of a low-power laser on the healing of periodontal tissue." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2003. http://hub.hku.hk/bib/B37651006.
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Moore, Edward Andrew. "Cell attachment and spreading on physical barriers used in periodontal guided tissue regeneration /." Oklahoma City : [s.n.], 2002. http://library.ouhsc.edu/epub/theses/Moore-William-A.pdf.
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B, Qasim Syed Saad. "Development of a novel functionally graded guided tissue regenerative membrane for periodontal lesions." Thesis, University of Sheffield, 2015. http://etheses.whiterose.ac.uk/11514/.
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Periodontal diseases are chronic inflammatory conditions affecting the supporting tissues of teeth caused by the prolonged accumulation of micro-organisms in the biofilm that forms on tooth surfaces. Conventional non-surgical and surgical treatments aim to halt disease progression and repair the lost periodontal tissues. Surgical therapies such as open flap debridement are aimed to replace the lost alveolar bone and guided tissue regeneration (GTR) is also used to treat this disease. Whilst the regeneration of lost support is an aim of periodontal treatment, the outcomes of current approaches to periodontal regeneration (PR) are unpredictable. Consequently, significant laboratory and clinical research has been undertaken to explore the possibilities of improving the outcomes of treatment over the past few decades. In this respect chitosan (CH), a well-known biopolymer holds promise to be fabricated in various forms. The aim of the project was to fabricate a trilayered functionally graded GTR membrane by fabricating surface and core layers of non-porous and porous morphologies with complete physiochemical and biological characterisation. Solvent Casting, Freeze gelation (FG) and Electrospinning was performed on CH alone and in combination with Hydroxyapatite (HA). Membranes were characterized with Scanning electron Microscopy,Fourier Transform Infrared Spectroscopy (FTIR), tensile testing, long term degradation and swelling studies were also performed. Cell culturing was performed using human osteosarcoma and progenitor cell line. Sirius red and Alizarin red assays were conducted to assess matrix deposition. Amongst the non-porous membranes fabricated by solvent casting, with Low molecular weight (LMw) CH:HA ratio of 30:70 showed better biocompatibility, and amongst the porous membranes made up of FG, ASa (ascorbic acid) :CH:HA (50:50) showed better stability and biocompatibility after in-vitro analysis. Histology of FG membranes conducted after in-vivo studies showed ASa:CH:HA to have higher cellular infiltration after 30 days of implantation. Electrospun fibres obtained in both aligned and random orientations were conducive to cellular attachment and mineralized matrix deposition with time. FTIR analysis showed strong co-ordination bond formation in between CH and HA. HA incorporated samples treated with simulated body fluid (SBF) showed an embryonic layer formation of hydroxyl carbonated apatite. Membranes can be combined together in different ways to achieve structural and functionally graded structures. A template was prepared using solvent casting and freeze gelation techniques to achieve functional gradients. Furthermore; CH and HA composite membranes could possibly be used for GTR applications in periodontal lesions and in addition these techniques could be further tuned to achieve desirable characteristics of a GTR membrane for PR and also holds promise to be used in other biomedical applications.
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Ochareon, Pannee. "Craniofacial periosteal cell capacities /." Thesis, Connect to this title online; UW restricted, 2004. http://hdl.handle.net/1773/6387.
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Ishikiriama, Bella Luna Colombini. "Estudo da expressão e produção de componentes do sistema renina-angiotensina por fibroblastos de gengiva e ligamento periodontal humanos." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/25/25142/tde-08022013-084557/.
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O Sistema Renina-angiotensina (SRA), e um sistema capaz de gerar hormonios peptideos com grande impacto na regulacao cardiovascular e na patogenese das doencas cardiovasculares. Este sistema opera, por meio das acoes da Angiotensina II, tanto em nivel sistemico (endocrino) quanto tecidual (local, paracrino/autocrino) controlando importantes funcoes, varias delas relacionadas a facilitacao da instalacao e progressao do processo inflamatorio. Por este motivo, a producao desta proteina nos tecidos pode estar relacionada a patogenese de muitas doencas, dentre elas a doenca periodontal (DP), tendo em vista seu carater infeccioso-inflamatorio e os achados da literatura que mostram que a inibicao da formacao de Ang II, diminui a perda óssea da DP em animais. Desta forma, o presente trabalho teve como objetivos: Avaliar in vitro, a) A expressao de componentes do SRA (ANGT, RENINA, ECA, ECA-2, AT1, AT2 e Mas) por fibroblastos de gengiva e ligamento periodontal humanos, por RT-qPCR; b) A producao de componentes do SRA (RENINA, ECA, ECA-2) no sobrenadante de culturas de fibroblastos de gengiva e ligamento periodontal humanos, por ELISA; c) A producao dos receptores do SRA (AT1, AT2 e Mas), nestes fibroblastos, por Imunofluorescencia e d) Se a expressao e a producao dos componentes do SRA por fibroblastos de gengiva e ligamento periodontal humanos, se alteram com a estimulacao por LPS de P. gingivalis e E. coli. Apos a coleta, os dados foram analisados com o auxilio do programa GraphPad Prism 5.0. por meio da analise de variancia a 2 criterios (ANOVA-two way) seguida do pos teste de Bonferroni, com nivel de significancia de 5% para a verificacao das possíveis diferencas. Foi detectada a expressao genica para alguns dos componentes do SRA (ANGT, RENINA, ECA, AT1) por fibroblastos tanto de gengiva quanto de ligamento periodontal. Foi detectada ainda uma expressao genica diferenciada entre fibroblastos de gengiva e ligamento periodontal para a ECA, sendo significativamente maior nos fibroblastos da gengiva. Houve imunomarcacao positiva tanto nos fibroblastos de gengiva quanto de ligamento periodontal compativel com a presenca dos receptores AT1 e Mas. Pode-se observar por fim que o contato com LPS de P. gingivalis e E. coli, na concentracao de 10 g/mL/24 h, nao alteram a expressão dos componentes do SRA. Portanto, pode-se concluir que os fibroblastos tanto de gengiva quanto de ligamento periodontal apesar de nao expressarem e produzirem todos oscomponentes do SRA necessarios para a formacao local de Ang II, poderiam contribuir, ainda que parcialmente, com outras celulas do microambiente dos tecidos periodontais para a formacao e acao locais da Ang II, e assim, para a instalacao e progressao da DP.The Renin-angiotensin system (RAS) can generate hormones that have a high-impact on cardiovascular regulation as well as in the pathogenesis of cardiovascular disease. This system acts through both systemic (endocrine) and local (paracrine/autocrine) effects of Angiotensin II, controlling important functions related to the facilitation of installation and progression of the inflammatory process. For this reason, this proteins production in tissues can be associated to the pathogenesis of many diseases, including periodontal disease (PD). In the PD setting, a infectious-inflammatory characterized disease, the literature findings shows that inhibition of the Ang II formation can decrease the bone loss in animals. In this context, the aims of the present study were: to investigate in vitro: a) the expression of RAS components (ANGT, RENIN, ECA, ECA- 2, AT1, AT2 and Mas) by human gingival and periodontal ligament fibroblasts by RT-qPCR; b) the production of RAS receptors (AT1, AT2 and Mas) by human cultured gingival and periodontal ligament fibroblasts by Immunofluorescence and d) the production of RAS components (RENIN, ECA, ECA-2) if the expression and production of RAS components by gingival and periodontal ligament fibroblasts modify under P. gingivalis and E. coli LPS stimulation. After collected, the data were analysed using GraphPad Prism 5.0, by the two way ANOVA followed by Bonferroni post test with a significance level of 5%. Gene expression was detected for some of the RAS components (ANGT, RENIN, ECA, AT1) by both gingival and periodontal ligament fibroblasts. It was detected a differential gene expression between gingival and periodontal ligament fibroblasts for ECA, being significantly higher in gingival fibroblasts. There was a stain in Immunofluorescence compatible with the production of RAS receptors (AT1 and Mas). It must be noted that the stimulation with P. gingivalis and E. coli LPS, in a concentration of 10 g/mL/24 h, did not altered the expression of RAS components. In conclusion, despite of neither gingival or periodontal ligament fibroblasts express all components of RAS, needed to local formation of Ang II, they might also contribute to the local formation and action of Ang II and in consequence, to the installation and the progression of DP.
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Mizuno, Hirokazu, Hideaki Kagami, Junji Mase, Daiki Mizuno, and Minoru Ueda. "Efficacy of Membranous Cultured Periosteum for the Treatment of Patients with Severe Periodontitis: a Proof-of-Concept Study." Nagoya University School of Medicine, 2010. http://hdl.handle.net/2237/12910.
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Belibasakis, Georgios N. "Cellular and molecular responses of periodontal connective tissue cells to Actinobacillus actinomycetemcomitans cytolethal distending toxin." Doctoral thesis, Umeå : Univ, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-345.
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Biederman, Poliana de Fátima. "Avaliação dos hormônios sexuais femininos sobre o tecido ósseo de ratas obesas submetidas à periodontite experimental." Universidade Estadual do Oeste do Parana, 2015. http://tede.unioeste.br:8080/tede/handle/tede/656.
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Previous issue date: 2015-05-26Obesity has been the subject of several controversies, whether in scientific circles or among the general population. Few subjects call so much attention to society and researchers, whether due to the huge number of obese people worldwide, or due to its potential complications to the health of individuals. Several chronic diseases have been associated with obesity among them periodontal diseases, which may be associated with bone resorption and have its mechanisms intensified in obesity. Nowadays, it is demonstrated that the bone tissue undergoes influence of obesity, so that female sex hormones seem an important link between obesity and bone mass gain in obese patients. Given the importance of obesity and its considerable relation to periodontal disease and bone tissue the aim of this study was to evaluate the effect of the hormonal influence on periodontal tissues of adult female mice in proestrus phase of the estrous cycle, with experimentally induced obesity, through the cafeteria diet, whether or not associated with periodontal disease. For this study 20 adult female mice cycling, in proestrus phase of the estrous cycle were divided into control group (CON), ligature group (LIG), cafeteria group (CAF) and cafeteria and ligature group (CAFLIG). At 75 days of life, the animals from groups LIG and CAFLIG received a ligature around the right mandibular first molar which acted as a gum irritating for 30 days, favoring the accumulation of bacterial plaque and the consequent development of periodontal disease. After euthanasia, at 105 days of life, it was held the blood collection from the right brain stem to determine the estradiol and progesterone concentrations by using specific kits; and the dissection of the right hemi mandible, which was submitted to histological analysis. The obtained data were analyzed and evaluated using the ANOVA and Tukey tests so that the results showed a decrease in the concentration of female sex hormones when obesity and periodontal disease were associated; in the same way it was observed an evident bone resorption and a change in the morphology of the bone tissue in the group CAF/LIG. Based on the results obtained the current study can conclude that the decrease in female sex hormones associated with obesity and periodontal disease negatively influenced on the mandibular bone tissue
A obesidade tem sido tema de diversas polêmicas e controvérsias, seja no meio científico ou na população em geral. Poucos assuntos chamam tanto a atenção da sociedade e de estudiosos, quer devido ao elevadíssimo número de pessoas obesas a nível mundial, quer devido às suas potenciais complicações para a saúde dos indivíduos. Diversas doenças crônicas têm sido relacionadas à obesidade, dentre elas as doenças periodontais, as quais podem estar associadas à reabsorção óssea e ter seus mecanismos intensificados na obesidade. Atualmente, demonstra-se que o tecido ósseo sofre influência da obesidade, sendo que os hormônios sexuais femininos parecem um importante elo entre a obesidade e o ganho de massa óssea em pacientes obesos. Dada a importância da obesidade e sua considerável relação com a doença periodontal e o tecido ósseo, o objetivo deste estudo foi avaliar o efeito da influência hormonal nos tecidos periodontais de ratas adultas na fase do proestro do ciclo estral, com obesidade induzida experimentalmente, através da dieta de cafeteria, associada ou não à doença periodontal. Para a realização da pesquisa, 20 ratas fêmeas adultas ciclando, na fase do proestro do ciclo estral, foram divididas em grupo controle (CON), grupo ligadura (LIG), grupo cafeteria (CAF) e grupo cafeteria e ligadura (CAFLIG). Aos 75 dias de vida, os animais dos grupos LIG e CAFLIG receberam uma ligadura ao redor do primeiro molar inferior direito, a qual atuou como irritante gengival por 30 dias, favorecendo o acúmulo de placa bacteriana e o consequente desenvolvimento da doença periodontal. Após a eutanásia, aos 105 dias de vida, realizou-se a coleta de sangue do tronco cerebral direito para a determinação das concentrações de estradiol e progesterona utilizando-se kits específicos; e a dissecação da hemi-mandíbula direita, que foi submetida à análise histológica. Os dados obtidos foram analisados e avaliados por meio dos testes ANOVA e Tukey, sendo que os resultados demonstraram uma diminuição na concentração dos hormônios sexuais femininos quando obesidade e doença periodontal foram associadas; da mesma forma, observou-se reabsorção óssea acentuada e mudança na morfologia do tecido ósseo no grupo CAFLIG. Com base nos resultados obtidos, pode-se concluir que a diminuição nos hormônios sexuais femininos associados à obesidade e à doença periodontal interferiu negativamente no tecido ósseo mandibular
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Gonçalves, Patricia Furtado. "Efeito do cemento dental na regeneração periodontal : um estudo histomorfometrico em cães." [s.n.], 2006. http://repositorio.unicamp.br/jspui/handle/REPOSIP/289036.
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Orientadores: Francisco Humberto Nociti Junior, Marcio Zaffalon CasatiTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: Tendo sido demonstrada a possibilidade de preservação do cemento como forma alternativa de tratamento da doença periodontal, o presente estudo teve como objetivo avaliar o efeito do cemento dental na regeneração periodontal. Foram utilizados nove cães, sem raça definida, que receberam ligaduras de fio de algodão nos terceiros pré-molares inferiores, bilateralmente, para produzir lesões de bifurcação classe III. Após o levantamento de retalhos mucoperiosteais, os defeitos foram aleatoriamente escolhidos para receber um dos seguintes tratamentos: (A) remoção do tecido de granulação e uma cuidadosa raspagem e alisamento radicular, utilizando curetas e brocas, objetivando a remoção dos depósitos duros e moles e do cemento radicular, e polimento radicular com taças de borracha e pasta profilática; (B) somente a remoção do tecido de granulação e polimento radicular com taças de borracha e pasta profilática, objetivando a preservação do cemento radicular. Em ambos os grupos, os defeitos foram tratados com o princípio da Regeneração Tecidual Guiada (membranas reabsorvíveis - RESOLUT XT®) e os retalhos foram posicionados coronariamente e suturados. Após quatro meses os animais foram sacrificados para uma análise histomorfométrica. No grupo B, houve uma maior extensão (3,59±1,67 e 6,20±2,26mm para os grupos A e B, respectivamente; p=0,004) e espessura (18,89±9,47 e 52,29±22,48µm para os grupos A e B, respectivamente; p=0,001) de novo cemento, uma maior extensão de novo osso (1,86±1,76 e 4,62±3,01mm para os grupos A e B, respectivamente; p=0,002), além de uma maior extensão de regeneração periodontal, quando comparado ao grupo A (p<0,05 ¿ Wilcoxon). Observou-se ainda, no grupo B, uma menor formação de tecido epitelial/conjuntivo sobre a superfície radicular (2,77±0,79 e 1,10±1,48mm para os grupos A e B, respectivamente; p=0,009), além de uma maior porcentagem de preenchimento da área do defeito (80,95±17,46 e 94,50±10,27 para os grupos A e B, respectivamente; p<0,05), com uma maior proporção de novo osso e uma menor proporção de tecido epitelial/conjuntivo (p<0,05). Dentro dos limites deste estudo, conclui-se que o cemento dental pode modular a regeneração periodontal
Abstract: The aim of the present study was to histometrically evaluate, in dogs, the effect of root cementum on periodontal regeneration. Nine mongrel dogs were used to obtain bilateral chronic class III furcation defects by placing cotton ligatures around both third mandibular premolars. The teeth were randomly assigned to receive one of the following treatments: (A) Scaling and root planning, by means of hand and rotatory instruments, in order to remove soft and hard deposits as well as all root cementum; (B) Removal of only soft microbial deposits, by polishing the root surface with rubber cups and polishing paste, aiming at maximum root cementum preservation. Both groups were treated with Guided Tissue Regeneration (GTR), with the use of resorbable poliglycolic-lactic-acid membranes (RESOLUT XT®) covering the buccal e lingual aspects of the furcation defect, and the flaps coronally positioned. Four months after the treatment, the animals were sacrificed and the histological sections obtained. Data analysis showed that a superior length (3.59±1.67 vs. 6.20±2.26mm; p=0.004) and a thicker layer (18.89±9.47 vs. 52.29 ±22.48µm; p=0.001) of new cementum were observed for group B (p<0.05). Additionally, group B presented and a superior length of new bone (1.86±1.76 vs. 4.62±3.01mm; p= 0.002) and less soft tissue along the root surface (2.77±0.79 vs. 1.10±1.48mm; p=0.009), and a larger proportion of periodontal regeneration when compared to control (p<0.05). A larger area of new bone (p=0.018) and a smaller area of soft tissue (p=0.024) were also observed for group B (p<0.05 - Wilcoxon). Within the limits of this study, it can be concluded that root cementum may modulate periodontal regeneration
Doutorado
Periodontia
Doutor em Clínica Odontológica
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Silva, Carla de Oliveira Pires da. "Avaliação da viabilidade tecidual de ligamento periodontal recém-extraído sob efeito de proteínas da matriz do esmalte e seu potencial angiogênico." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-23092016-151750/.
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A periodontite é uma doença inflamatória crônica, multifatorial, altamente prevalente na população que compromete os tecidos de suporte dos dentes gerando sequelas de difícil resolução, mesmo com as mais modernas técnicas regenerativas. A terapia tecidual parece ser uma alternativa promissora e as células indiferenciadas do ligamento periodontal (PDL) tem demonstrado grande potencial terapêutico. No entanto, o PDL necessita de estímulos adequados de biomodificadores para que a diferenciação ocorra de forma coordenada. As proteínas da matriz do esmalte (EMD) é um tipo de biomodificador que promove formação de novo cemento. Apesar de ser utilizada clinicamente, a sua associação com células frescas do PDL ainda não foi explorada. Este é um estudo da expressão gênica e proteica do PDL com finalidade de reaproveitamento do tecido recém-extraído, estimulado por EMD, tendo em vista a regeneração periodontal. Os resultados da expressão gênica apresentam VEGF (p=0.5194) com diferença entre medianas de -0.201 e FGF-2 (p = 0,0059) com diferença entre medianas de -0.4167. No estudo de citocinas, VEGF-A (p<0,0001) com diferença entre medianas de 60,93, enquanto VEGF-D (p=0.0049) com entre medianas de 2,45. Através dos resultados da expressão gênica baseada em FGF-2 e proteica baseada em VEGF-A, foi possível observar que as EMD modularam a resposta tecidual ex-vivo no período de 10 minutos tendo em vista o padrão angiogênico. Essa combinação poderá servir como uma proposta terapêutica, visando a aplicação clínica futura de tecidos comumente descartados após exodontia, como o PDL.Periodontitis is a chronic inflammatory disease, multifactorial and highly prevalent in the world population that affects the teeth of the supporting tissues, generating sequels difficult to solve even with the most modern regenerative techniques. Tissue therapy appears to be a promising alternative and undifferentiated cells of the periodontal ligament (PDL) have shown great therapeutic potential. However, the PDL needs to appropriate stimuli biomodificatores that differentiation occurs in a coordinated fashion. The enamel matrix proteins (EMP) are a type of biomodificator that promotes new cementum formation. Despite being used clinically, its association with PDL fresh cells has not yet been explored. This is a study of gene and protein expression of PDL with reuse purpose of the newly extracted tissue stimulated by EMD, with a view to periodontal regeneration. The results show VEGF gene expression (p = 0.5194) difference in median -001 and FGF-2 (p = 0.0059) difference in medians of -0.4167. In the study of cytokines, VEGF-A (p <0.0001) with the difference between medians of 60.93, whereas VEGF-D (p = 0.0049) with from 2.45 medians. Through the results of the FGF-2-based gene expression and protein-based VEGF-A, it was observed that the EMD modulated the tissue response ex vivo in the 10-minute period considertingthe standard angiogenic. This combination may serve as a therapeutic approach aimed at future clinical application of tissues commonly discarded after extraction, such as the PDL.
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Tang, Margaret P. F. "A morphometric transmission electron microscopic study of tissue channels in normal and tensioned rat molar periodontal ligament /." Title page, contents and summary only, 1991. http://web4.library.adelaide.edu.au/theses/09DM/09dmt164.pdf.
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Rinehart, Sarah. "Periodontal phenotype and supracrestal soft tissue dimensions – clinical correlations and their impact on post-extraction volumetric changes." Thesis, University of Iowa, 2017. https://ir.uiowa.edu/etd/5834.
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Objectives:
Extraction of a tooth leads to a series of healing events that are intimately associated with dimensional changes in the alveolar ridge that typically result in a net volume loss. Previous studies have evaluated the extent and pattern of those resorptive changes, however it remains challenging to predict the degree of change that will occur, as numerous local and systemic factors may play a role in the biologic events that follow tooth extraction. The purpose of this study was to assess the role that phenotypic characteristics of the periodontium play in the alveolar ridge remodeling processes that take place following single tooth extraction.
Methods:
Healthy patients in need of a single tooth extraction in the maxillary arch from second premolar to second premolar (inclusive) and who met a predefined eligibility criteria were enrolled in this study. An impression of the maxillary arch was made and a cone beam computed tomography (CBCT) scan of the maxilla was obtained immediately prior to tooth extraction at the baseline visit. At the time of the extraction, clinical measurements were made including probing depth, bone sounding, buccal keratinized mucosa width, buccal and palatal alveolar bone thickness, and buccal and palatal soft tissue thickness. Fourteen weeks following the baseline intervention, patients returned to the clinic for a second impression of the maxillary arch and a second CBCT of the maxilla. Linear and volumetric bone measurements were made using the data obtained from the CBCT scans. The casts obtained from the impressions were digitally scanned and volumetric measurements were made from the digitized data to assess volume changes of the residual ridge. The primary outcome of interest was the volumetric percent reduction of the alveolar ridge following single tooth extraction. Spearman correlations were utilized to evaluate relationships between variables and modeling was completed to predict the percentage of volumetric change in the hard and soft tissues using the clinical variables.
Results:
A total of 21 patients participated in the study, 19 patients are included in this analysis (one patient has yet to complete the study, one patient was later excluded due to lack of compliance). Of the 19 extraction sites included, 17 were maxillary premolar teeth. At baseline, the average buccal plate thickness was 1.09 mm. After 14 weeks, the average loss of alveolar bone width was 1.66 mm. The average loss of buccal bone height was 1.10 mm and mean loss of palatal bone height was 1.36 mm. The average percentage volumetric reduction of the bone as measured from a CBCT scan was 26.42% after 14 weeks of healing. Mean percentage volumetric reduction of the ridge, as measured from a digitized cast, was 18.89%. There was no statistically significant correlation noted between the bone and ridge volumetric measurements.
While there were no statistically significant correlations noted between the thickness of the buccal bone and the amount of volumetric remodeling, statistically significant negative correlations were found between the buccal bone thickness and the loss of alveolar bone width (rs = -0.66418, p-value = 0.0019). In addition, a statistically significant correlation was noted between the reduction in alveolar bone width and the loss of buccal ridge height (rs = 0.55707, p-value = 0.0132). Modeling methods found that increased thickness of the buccal soft tissue was predictive of increased percentage volumetric reduction of hard tissues (coeff = 37.24, p-value = 0.0301).
Conclusions:
Increased buccal soft tissue thickness was found to be predictive of increased percent volumetric reduction of alveolar bone. Thinner buccal bone was correlated with increased loss of alveolar bone width. While statistically significant correlations were identified, further studies with larger sample size are needed to better understand these relationships.
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Reino, Danilo Maeda. "Tratamento de retrações gengivais em pacientes fumantes graves através de duas técnicas cirúrgicas: estudo clínico, laboratorial e histológico." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-19032010-105758/.
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Objetivo: Este trabalho avaliou o recobrimento radicular obtido por duas técnicas de cirurgia plástica periodontal em pacientes fumantes graves, através de análises clínica, histológica e laboratorial. Materiais e Métodos: Foram selecionados 20 pacientes fumantes graves que apresentavam ao menos duas retrações classes I ou II de Miller em dentes não molares, bilateralmente. Os parâmetros clínicos avaliados foram: profundidade de sondagem (PS), nível clínico de inserção (NCI), altura (AR) e largura das retrações (LR), altura (AM) e espessura da mucosa queratinizada (EM) e altura e base das papilas mesiais e distais à retração. Um lado recebeu retalho posicionado coronalmente, enquanto no outro lado foi utilizada a técnica de retalho estendido. Ambos os procedimentos foram associados ao enxerto de tecido conjuntivo subepitelial. Biópsias foram feitas durante os procedimentos cirúrgicos para quantificação dos vasos sanguíneos utilizando de avaliação imunohistoquímica. Coleta de saliva foi realizada pré tratamento e após 3 e 6 meses, como forma de avaliar, por meio de ensaio imunoenzimático (ELISA), a exposição ao fumo durante o período completo do estudo. Resultados: Os parâmetros clínicos avaliados não apresentaram diferença estatística entre grupos ou intra-grupos. Os pacientes mantiveram a mesma exposição ao fumo durante os 6 meses avaliados. O recobrimento radicular obtido foi baixo para ambas as técnicas estudadas (43,18% para técnica padrão e 44,52% para técnica teste). O retalho apresentou menor quantidade de vasos sanguíneos do que o tecido do enxerto (13,49 ± 5,48 contra 18,69 ± 4,87, p= 0,0001). Conclusão: O fumo prejudicou intensamente os resultados de ambas as técnicas empregadas, reduzindo o recobrimento radicular, a frequência de recobrimento total e a quantidade de vasos sanguíneos nos tecidos. Não houve diferença entre as técnicas testadas.Background: The aim of this study was to evaluate the root coverage obtained by two different periodontal plastic techniques in severe smokers, analyzing clinical, laboratorial and histological parameters. Methods: Twenty severe smokers were selected. Each patient had bilateral Miller class I or II gingival recessions in non-molar teeth. Clinical measurements of probing pocket depth (PPD), clinical attachment level (CAL), recession height (RH) and recession width (RW), keratinized mucosa height (KMH) and keratinized mucosa thickness of (KMT) and height and width of the adjacent papillae were determined at baseline, 3 and 6 months. One side received coronally positioned flap, while the other side received the extended flap technique, both procedures in conjuction with the subeptelial connective tissue graft (SCTG). Biopsies from SCTGs and flaps were prepared for immunohistochemistry. Blood vessels were identified and counted by coexpression of Factor-VIII related antigen stained endothelial cells. Salivary cotinine samples were taken at baseline and after 3 and 6 months, as an indicator of the level of exposure to nicotine. Results: Between groups or intra groups analysis showed no statistical differences for the evaluated clinical parameters. Patients maintained the same exposure to smoke during the 6 months evaluation periods. Both techniques had low root coverage (standard technique: 43.18% and test technique: 44.52%). Histomorphometry of biopsies revealed blood vessel densities of 13.49 ± 5.48 vessels/200x field for the flaps, and of 18.69 ± 4.87 vessels/200x field for the grafts (p = 0.0001). Conclusion: The extend flap technique did not differ from standart technique for recession treatment of severe smoking patients.
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Heinze, Joseph John. "A retrospective study of the treatment of grade II furcations with a tricomponent bioresorbable membrane." Oklahoma City : [s.n.], 2006.
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Bianucci, Henri Chapman Jr. "Periodontal Healing of Canine Experimental Grade III Furcation Defects Treated With Autologous Fibrinogen and Resolut® Barrier Membrane." Thesis, Virginia Tech, 1998. http://hdl.handle.net/10919/36760.
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Experimental grade III furcation defects were developed bilaterally at the second and fourth premolars in 18 conditioned, laboratory-source, adult Beagles and maintained for 12 weeks. All defects were treated with debridement and mucoperiosteal flap repositioning alone, or with the addition of autologous fibrinogen, Resolut® barrier membrane, or autologous fibrinogen and Resolut® barrier membrane. The defects were analyzed by digital subtraction radiography, histopathologic, and histomorphometric analysis of healing at 1, 3, and 6 months post-treatment to determine: percent increase in defect bone volume, height and area, and length of periodontal regeneration along the perimeter of the defect. Comparisons at post-treatment intervals indicated significantly (P < 0.05) greater healing of debridement and autologous fibrinogen treated defects at 3 months, however by 6 months there were no significant differences in defect healing for all histomorphometric parameters. Defects receiving Resolut® were associated with significantly less root ankylosis. Defects receiving debridement alone had significantly greater increases in bone volume, as calculated with digital subtraction radiography, at 6 months post-treatment compared with groups receiving Resolut®. There was a significant correlation between regenerated bone area, bone volume, and periodontal regeneration for all treatments at 3 and 6 months post-treatment.
In this study autologous fibrinogen and Resolut® barrier membrane did not enhance the amount of periodontal healing compared with debridement only. However, Resolut® treated defects were essentially absent of root ankylosis. Therefore, canine periodontitis causing grade III furcation involvement may respond equally well to conservative periodontal surgery compared with guided tissue regenerative techniques. However, the prevention of root ankylosis may provide a substantial benefit favoring this latter methodology.Master of Science
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Martinez, Catalina. "The Effects of Dynamic Culturing Environments on Cell Populations Relevant to Heart Valve Tissue Engineering." FIU Digital Commons, 2011. http://digitalcommons.fiu.edu/etd/505.
Full textAbstract:
The design of a tissue engineered pulmonary valve (TEPV) involves cells source(s), scaffold, in vitro conditioning system and the functional stability of the TEPV in vivo. Vascular cells (pulmonary artery smooth muscle (SMCs) and endothelial cells (ECs)) and periodontal ligament derived stem cells (PDLSCs) are relevant sources for the designing of TEPVs. In this study, labeling of these cell populations with super paramagnetic iron oxide microparticles along with concomitant usage of transfection agents was followed by visualization using magnetic resonance, while Intracellular iron oxide was confirmed by prussian blue staining and fluorescence microscopy. Also, the potential of PDLSC as a feasible source for TEPVs was investigated, expressing differentiative capacity to both SMC and EC phenotypes by a combination of biochemical and mechanical stimulation. Flow conditioning in a u-shaped bioreactor augmented collagen production in SMC-EC (99.5% for n=3) and PDLSC (93.3% for n=3) seeded scaffolds after a 3-week culturing period (P<0.05).
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Silva, Henrique Aparecido Bueno da. "Expressão do receptor ativado por protease do tipo 1 (PAR-1) em pacientes com periodontite crônica." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/23/23146/tde-28032014-193229/.
Full textAbstract:
A ativação do receptor ativado por protease do tipo 1 (PAR-1) pela trombina desempenha um papel fundamental na deposição de matriz vascular após injúria tecidual, reparação óssea e na homeostase dos tecidos periodontais, assim como na proliferação de fibroblastos gengivais. O principal objetivo deste estudo foi investigar a expressão de PAR-1 em pacientes com periodontite crônica, antes e 45 dias após tratamento periodontal não-cirúrgico. Amostras de fluido gengival (FG), de saliva e parâmetros clínicos, como profundidade de sondagem (PS), perda do nível clínico de inserção (NCI), sangramento à sondagem (SS), índice gengival (IG) e índice de placa (IP) foram coletados de pacientes com saúde periodontal (Controle) e pacientes com periodontite crônica moderada antes (PC) e 45 dias após o tratamento não-cirúrgico periodontal (PCT). A expressão gênica de PAR-1 (mRNA) em FG foram avaliadas por qPCR (Reação de Polimerase em Cadeia em Tempo Real). A análise por citometria de fluxo foi realizada para identificar quais células expressam PAR-1 em FG. Biomarcadores inflamatórios salivares também foram determinados. Os parâmetros clínicos foram significativamente melhorados após terapia periodontal não-cirúrgica (p<0,01). A análise por qPCR mostrou que antes da terapia, níveis de PAR-1 (mRNA) em periodontite crônica foram menores aos controles. O tratamento periodontal levou ao aumento da expressão de PAR-1 em periodontite crônica (p<0,05). A expressão de PAR-1 foi inversamente correlacionada com a expressão dos níveis salivares de IL-6, IL-8, TNF-, IFN-, e MMP-2. Em conclusão, o presente trabalho demonstrou que a expressão de PAR-1 aumentou após o tratamento periodontal em células do FG, e que a expressão de PAR-1 está associada com a diminuição da expressão dos níveis salivares de biomarcadores inflamatórios. Portanto, dentro dos limites do presente estudo, nossos dados sugerem a importância do papel mediador de PAR-1 na reparação do tecido periodontal.Activation of protease-activated receptor type 1 (PAR-1) thrombin plays a key role in matrix deposition after vascular tissue injury, bone repair and homeostasis of periodontal tissues, as well as the proliferation of gingival fibroblasts. The main objective of this study was to investigate the expression of PAR-1 in patients with chronic periodontitis before and 45 days after non-surgical periodontal treatment. Samples of gingival fluid (GF), saliva and clinical parameters such as probing depth (PD), loss of clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were collected from patients with periodontal health (Control) and patients with moderate chronic periodontitis before (PC) and 45 days after non-surgical periodontal treatment (PCT). The gene expression of PAR-1 (mRNA) in GF were assessed by qPCR (Polymerase Chain Reaction in Real Time). The flow cytometry analysis was performed to identify cells which express PAR-1 in GF. Salivary inflammatory biomarkers were also determined. Clinical parameters were significantly improved after therapy (p<0,01). The qPCR analysis showed that before therapy, PAR-1 (mRNA) levels in chronic periodontitis were smallest to controls. Periodontal treatment led to increased PAR-1 expression in chronic periodontitis (p<0,05). PAR-1 expression was inversely correlated to IL-6, IL-8, TNF-, IFN-, and MMP-2 salivary levels. In conclusion, the present study showed that the expression of PAR-1 increased after periodontal treatment in FG cells, and that expression of PAR-1 is associated with decreased expression of salivary levels of inflammatory biomarkers. Therefore, within the limits of this study, our data suggest the importance of the mediating role of PAR-1 in the repair of periodontal tissue.
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Ruiz, Karina Gonzales Silvério. "Efeitos da combinação do fator de crescimento de fibroblastos básico e fator de transformação de crescimento beta na proliferação, expressão de genes para colágeno tipos I e III, metaloproteases-1 e -2 e TIMPs 1,2 e 3, e na modulação da síntese destes próprios fatores de crescimento pelas células do ligamento periodontal de humanos /." Araraquara : [s.n.], 2005. http://hdl.handle.net/11449/104749.
Full textAbstract:
Orientador: Ricardo Samih Georges Abi RachedBanca: Silvana Regina Perez Orrico
Banca: Regina Maria Barretto Cicarelli
Banca: Francisco Humberto Nociti Junior
Banca: Márcio Zafallon Casati
Resumo: O objetivo do presente estudo foi avaliar o efeito da combinação do fator de crescimento de fibroblastos básico (b-FGF) e fator de transformação de crescimento beta (TGF- beta) na proliferação, expressão de genes para colágeno tipos I e III, metaloproteases -1 e -2 e TIMPs 1, 2 e 3, e na síntese destes próprios fatores de crescimento pelas células do ligamento periodontal de humanos. A avaliação da proliferação celular foi realizada após os períodos de 24 e 48h na presença dos fatores de crescimento, através da mensuração do nível de MTS reduzido a formazan pelas células viáveis, e a síntese de b-FGF e TGF-b pelo teste ELISA empregando a técnica "sandwich". Conclui-se que as associações do bFGF e do TGF-b atuaram de maneira dose-dependente no estímulo à proliferação celular, o aumento na expressão de genes para colágeno e TIMPs e redução para as metalproteases e modulação da síntese deles próprios.
Abstract: The aim of this study was to evaluate the effect of association of basic fibroblast growth factor and transforming growth factor beta on the proliferation, expression of colagen type I e III, matrix metalloproteinases-1 e -2 e TIMPs 1, 2 e 3, and on the modulation of the syntheses oh these growth factors by humans periodontal ligament cells. The cellular proliferation was evaluated to 24 and 48 hours of incubation by the colorimetric method. The synthese of bFGF and TGF-ß was verificated by ELISA method, using a "sandwich" techinique, and mRNA expression by Real Time - PCR. In conlusion, the associations of bFGF e TGF-ß influenced of dose-dependent manner on the cellular proliferation, on the increasing of the expression to collagen and TIMPs, and on the reduction to metaloproteinases and, the modulation of these own synthesis.
Doutor
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Martins, Thiago Marchi. "Cicatrização de defeitos de deiscência periodontal associados ao aplainamento radicular tratados com enxerto de tecido conj untivo subepitelial ou membrana bioabsorvível : análise histológica e histométrica em cães /." Araçatuba : [s.n.], 2009. http://hdl.handle.net/11449/104712.
Full textAbstract:
Orientador: Álvaro Francisco BoscoBanca: Maria José Hitomi Nagata
Banca: Valdir Gouveia Garcia
Banca: Mário Taba Júnior
Banca: Marcio Zaffalon Casati
Resumo: O objetivo do presente estudo foi avaliar histológica e histometricamente a cicatrização de defeitos de deiscência periodontal associados ao aplainamento radicular tratados com enxerto de tecido conjuntivo subepitelial (ETC) ou Membrana Bioabsorvível (MB), cada qual comparado ao tratamento com Retalho Posicionado Coronal (RPC). Foram utilizados 12 cães, divididos em dois grupos de 6. Defeitos de deiscência óssea (6x8 mm) e o aplainamento radicular foram realizados nos caninos superiores e os grupos divididos, seguindo um modelo de boca dividida de acordo com o tratamento. Os caninos esquerdos serviram como controle (RPC), onde o retalho foi somente posicionado coronalmente. Os caninos direitos receberam os tratamentos com ETC ou MB. Após 3 meses pósoperatórios os animais foram submetidos à eutanásia e os blocos processados para análise histológica e histométrica. Os parâmetros histométricos avaliados incluíram extensão de tecido epitelial (TE), extensão da nova inserção de tecido conjuntivo (NITC) e extensão da aposição de tecido conjuntivo (ATC), extensão de novo cemento (NC) e extensão de novo osso (NO). Histologicamente, o grupo MB mostrou significativamente maiores NC, NO e NITC (4.12±1.24; 2.44±0.78; 1.80±0.39, respectivamente) em comparação ao grupo RPC (1.04±0.43; 0.93±0.23; 0.71±0.45, respectivamente) (P<0.05) e significativamente menor ATC (0.29±0.33) comparado aos grupos RPC (2.79±1.74) e ETC (2.62±1.52) (P<0.05). A NITC no grupo ETC (0.71±0.36) foi significativamente menor, enquanto a ATC (2.62±1.52) e TE (1.70±0.53) foram significativamente maiores comparado ao grupo MB (1.80±0.39; 0.29±0.33; 1.00±0.35, respectivamente) (P<0.05). Dentro dos limites deste estudo, concluiuse que o grupo MB mostrou melhores resultados histológicos de nova inserção de tecido conjuntivo, formação de novo osso e novo cemento em relação aos grupos RPC e ETC.
Abstract: The aim of the present study was to evaluate histologically and histometrically the healing of periodontal dehiscence defects associated to root planing treated with subepithelial connective tissue graft (CTG) or Bioabsorbable Membrane (BM), each one compared to the treatment with Coronally Positioned Flap (CPF). Twelve dogs were used, divided into two groups of six elements. Bone dehiscence defects (6x8 mm) and root planing were accomplished in the upper canine and the groups were divided, following a model of divided mouth according to the treatment used. Left canine were used as control (CPF), where the flap was only coronally positioned. Right canine received treatments with CTG or BM. After three postoperative months, the animals were euthanized and the blocks were processed for histological and histometrical analysis. The evaluated histometric parameters included lenght of epithelial tissue (TE), new attachment (NACT) and connective tissue aposition (CTA), new cementum (NC), and new bone (NB). Histologically, group BM showed significant higher values of NC, NO and NITC (4.12±1.24; 2.44±0.78; 1.80±0.39, respectively) in comparison to the group CPF (1.04±0.43; 0.93±0.23; 0.71±0.45, respectively) (P<0.05) and significant lower values of ATC (0.29±0.33) in comparison to the groups CPF (2.79±1.74) and CTG (2.62±1.52) (P<0.05). NITC in group CTG (0.71±0.36) was significantly lower, while ATC (2.62±1.52) and TE (1.70±0.53) were significantly higher in comparison to group BM (1.80±0.39; 0.29±0.33; 1.00±0.35, respectively) (P<0.05). Within the limits of this study, it can be concluded that group BM showed better histological results of connective tissue new attachment, new bone and new cementum formation in comparison to groups CPF and CTG.
Doutor
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Delima, Suzanne Lynn. "Pre-Wounding and Free Gingival Grafts: A Pilot Investigation." The Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1372075336.
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Martins, Thiago Marchi [UNESP]. "Cicatrização de defeitos de deiscência periodontal associados ao aplainamento radicular tratados com enxerto de tecido conj untivo subepitelial ou membrana bioabsorvível: análise histológica e histométrica em cães." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/104712.
Full textAbstract:
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martins_tm_dr_araca.pdf: 622083 bytes, checksum: b164d76ff1eb9d604ddcefbda1146d1a (MD5)O objetivo do presente estudo foi avaliar histológica e histometricamente a cicatrização de defeitos de deiscência periodontal associados ao aplainamento radicular tratados com enxerto de tecido conjuntivo subepitelial (ETC) ou Membrana Bioabsorvível (MB), cada qual comparado ao tratamento com Retalho Posicionado Coronal (RPC). Foram utilizados 12 cães, divididos em dois grupos de 6. Defeitos de deiscência óssea (6x8 mm) e o aplainamento radicular foram realizados nos caninos superiores e os grupos divididos, seguindo um modelo de boca dividida de acordo com o tratamento. Os caninos esquerdos serviram como controle (RPC), onde o retalho foi somente posicionado coronalmente. Os caninos direitos receberam os tratamentos com ETC ou MB. Após 3 meses pósoperatórios os animais foram submetidos à eutanásia e os blocos processados para análise histológica e histométrica. Os parâmetros histométricos avaliados incluíram extensão de tecido epitelial (TE), extensão da nova inserção de tecido conjuntivo (NITC) e extensão da aposição de tecido conjuntivo (ATC), extensão de novo cemento (NC) e extensão de novo osso (NO). Histologicamente, o grupo MB mostrou significativamente maiores NC, NO e NITC (4.12±1.24; 2.44±0.78; 1.80±0.39, respectivamente) em comparação ao grupo RPC (1.04±0.43; 0.93±0.23; 0.71±0.45, respectivamente) (P<0.05) e significativamente menor ATC (0.29±0.33) comparado aos grupos RPC (2.79±1.74) e ETC (2.62±1.52) (P<0.05). A NITC no grupo ETC (0.71±0.36) foi significativamente menor, enquanto a ATC (2.62±1.52) e TE (1.70±0.53) foram significativamente maiores comparado ao grupo MB (1.80±0.39; 0.29±0.33; 1.00±0.35, respectivamente) (P<0.05). Dentro dos limites deste estudo, concluiuse que o grupo MB mostrou melhores resultados histológicos de nova inserção de tecido conjuntivo, formação de novo osso e novo cemento em relação aos grupos RPC e ETC.
The aim of the present study was to evaluate histologically and histometrically the healing of periodontal dehiscence defects associated to root planing treated with subepithelial connective tissue graft (CTG) or Bioabsorbable Membrane (BM), each one compared to the treatment with Coronally Positioned Flap (CPF). Twelve dogs were used, divided into two groups of six elements. Bone dehiscence defects (6x8 mm) and root planing were accomplished in the upper canine and the groups were divided, following a model of divided mouth according to the treatment used. Left canine were used as control (CPF), where the flap was only coronally positioned. Right canine received treatments with CTG or BM. After three postoperative months, the animals were euthanized and the blocks were processed for histological and histometrical analysis. The evaluated histometric parameters included lenght of epithelial tissue (TE), new attachment (NACT) and connective tissue aposition (CTA), new cementum (NC), and new bone (NB). Histologically, group BM showed significant higher values of NC, NO and NITC (4.12±1.24; 2.44±0.78; 1.80±0.39, respectively) in comparison to the group CPF (1.04±0.43; 0.93±0.23; 0.71±0.45, respectively) (P<0.05) and significant lower values of ATC (0.29±0.33) in comparison to the groups CPF (2.79±1.74) and CTG (2.62±1.52) (P<0.05). NITC in group CTG (0.71±0.36) was significantly lower, while ATC (2.62±1.52) and TE (1.70±0.53) were significantly higher in comparison to group BM (1.80±0.39; 0.29±0.33; 1.00±0.35, respectively) (P<0.05). Within the limits of this study, it can be concluded that group BM showed better histological results of connective tissue new attachment, new bone and new cementum formation in comparison to groups CPF and CTG.
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Esper, Luis Augusto. "Influência do plasma rico em plaquetas associado ou não à regeneração tecidual guiada na cicatrização de defeitos de fenestração periodontal em cães : estudo histológico e histométrico /." Araçatuba, 2009. http://hdl.handle.net/11449/96169.
Full textAbstract:
Orientador: Maria José Hitomi NagataBanca: Álvaro Francisco Bosco
Banca: Sonia Regina Panzarini Barioni
Resumo: Este estudo avaliou histomorfometricamente o processo de cicatrização em defeitos de fenestração periodontal, criados cirurgicamente em cães e tratados com Plasma Rico em Plaquetas (PRP) associado ou não ao uso de barreira de membrana. Defeitos de fenestração periodontal com 5 mm de diâmetro foram cirurgicamente criados nos caninos superiores de 12 cães. Os dentes foram divididos em 4 grupos: C (controle) - defeito preenchido somente com coágulo sangüíneo; M - defeito preenchido com coágulo sangüíneo e protegido com uma membrana de politetrafluoretileno expandido (PTFE-e) com reforço de titânio; PRP - defeito preenchido com PRP; PRP/M - defeito preenchido com PRP e protegido com uma membrana de PTFE-e com reforço de titânio. Os animais foram eutanasiados após 4 semanas. Medidas lineares e de área da cicatrização periodontal foram avaliadas e calculadas como porcentagem do defeito original. Os dados foram submetidos à análise estatística (análise de variância, p < 0,05). Nenhum espécime regenerou-se completamente com osso ou cemento. Formação de novo cemento foi significativamente maior nos Grupos PRP e PRP/M quando comparados ao Grupo C. Observou-se, também, significativa maior formação de novo cemento no Grupo PRP/M que no Grupo M. Dentro dos limites deste estudo, pode-se concluir que o PRP favoreceu a formação de novo cemento. A Regeneração Tecidual Guiada (RTG) não proporcionou efeitos adicionais ao uso do PRP no tratamento de defeitos de fenestração periodontal em cães.
Abstract: This study histomorphometrically analyzed the healing of periodontal fenestration defects surgically created in dogs and treated with Platelet-Rich Plasma (PRP) with or without a barrier membrane. A 5 mm diameter periodontal fenestration defect was made in each upper canine of 12 dogs. The teeth were divided into 4 groups: C (control) - defect filled with blood clot only; M - defect filled with blood clot and covered by a titanium-reinforced expanded polytetrafluoroethylene membrane (ePTFE); PRP - defect filled with PRP; PRP/M - defect filled with PRP and covered by a titanium-reinforced ePTFE. All animals were euthanized at 4 weeks post-operative. Linear and area measurements of periodontal healing were evaluated and calculated as a percentage of the original defect. Data were statistically analyzed (analysis of variance, p < 0.05). No defect completely regenerated with either bone or cementum. Cementum formation was significantly greater in groups PRP and PRP/M when both groups were compared to Group C. A significant greater cementum formation was also observed in Group PRP/M than in Group M. Within the limits of this study, it can be concluded that the PRP favored cementum formation. Guided Tissue Regeneration (GTR) did not promote any additional benefit to the use of PRP in the treatment of periodontal fenestration defects in dogs.
Mestre
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Ruiz, Karina Gonzales Silvério [UNESP]. "Efeitos da combinação do fator de crescimento de fibroblastos básico e fator de transformação de crescimento beta na proliferação, expressão de genes para colágeno tipos I e III, metaloproteases-1 e -2 e TIMPs 1,2 e 3, e na modulação da síntese destes próprios fatores de crescimento pelas células do ligamento periodontal de humanos." Universidade Estadual Paulista (UNESP), 2005. http://hdl.handle.net/11449/104749.
Full textAbstract:
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O objetivo do presente estudo foi avaliar o efeito da combinação do fator de crescimento de fibroblastos básico (b-FGF) e fator de transformação de crescimento beta (TGF- beta) na proliferação, expressão de genes para colágeno tipos I e III, metaloproteases -1 e -2 e TIMPs 1, 2 e 3, e na síntese destes próprios fatores de crescimento pelas células do ligamento periodontal de humanos. A avaliação da proliferação celular foi realizada após os períodos de 24 e 48h na presença dos fatores de crescimento, através da mensuração do nível de MTS reduzido a formazan pelas células viáveis, e a síntese de b-FGF e TGF-b pelo teste ELISA empregando a técnica sandwich. Conclui-se que as associações do bFGF e do TGF-b atuaram de maneira dose-dependente no estímulo à proliferação celular, o aumento na expressão de genes para colágeno e TIMPs e redução para as metalproteases e modulação da síntese deles próprios.
The aim of this study was to evaluate the effect of association of basic fibroblast growth factor and transforming growth factor beta on the proliferation, expression of colagen type I e III, matrix metalloproteinases-1 e -2 e TIMPs 1, 2 e 3, and on the modulation of the syntheses oh these growth factors by humans periodontal ligament cells. The cellular proliferation was evaluated to 24 and 48 hours of incubation by the colorimetric method. The synthese of bFGF and TGF-ß was verificated by ELISA method, using a sandwich techinique, and mRNA expression by Real Time - PCR. In conlusion, the associations of bFGF e TGF-ß influenced of dose-dependent manner on the cellular proliferation, on the increasing of the expression to collagen and TIMPs, and on the reduction to metaloproteinases and, the modulation of these own synthesis.
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Bonfante, Samara. "Padrão de cicatrização de defeitos de deiscência periodontal tratados com enxerto de tecido conjuntivo subepitelial ou matriz dérmica acelular : estudo histológico e histométrico em cães /." Araçatuba : [s.n.], 2008. http://hdl.handle.net/11449/104706.
Full textAbstract:
Orientador: Álvaro Francisco BoscoBanca: Valdir Gouveia Garcia
Banca: Maria José Hitomi Nagata
Banca: Márcio Zaffalon Casati
Banca: Márcio Fernando de Moraes Grisi
Resumo: O objetivo do presente estudo foi avaliar histológica e histometricamente o padrão de cicatrização de defeitos de deiscência periodontal tratados com enxerto de tecido conjuntivo subepitelial (ETC) ou matriz dérmica acelular (MDA), cada qual comparado ao tratamento com Retalho posicionado Coronal (RPC). Foram utilizados 10 cães, divididos em dois grupo de 5. Defeitos de deiscência óssea (6x8 mm) foram criados nos caninos superiores e os grupos divididos seguindo um modelo de boca dividida de acordo com o tratamento. Em um grupo de 5 cães foi realizado o tratamento com ETC e no lado contralateral RPC e o outro grupo de 5 cães foi tratado com MDA e RPC no lado contralateral. Após 3 meses pós-operatórios os animais foram submetidos à eutanásia e os blocos processados para análise histológica e histométrica. Os parâmetros histométricos avaliados incluíram extensão de tecido epitelial (TE), nova inserção (NITC) e aposição de tecido conjuntivo (ATC), novo cemento (NC) e novo osso (NO). Os dados histométricos transformados em porcentagem, foram estatisticamente analisados pelo teste ANOVA, seguido pelo teste de Tukey... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The aim of the present study was to evaluate histologically and histometrically the healing pattern of periodontal dehiscence defects treated with Subepithelial Connective Tissue Graft (CTG) or Acellular Dermal Matrix (ADM), when compared to the Coronally Positioned Flap (CPF). Dehiscence bone defects (6x8mm) were created bilaterally in the maxillary canine area of dogs (n=10, divided in 2 groups). By means of a split mouth test design, a group of 5 dogs was randomly allocated to receive subepithelial connective tissue graft (CTG) in one of the sides and a coronally positioned flap (CPF) in the opposing side. The remaining 5 dogs had acellular dermal matrix graft (ADM) as a treatment in either one of the sides and CPF on the other side. Three months after surgery, dogs were euthanized and blocks of interest were processed. The histometric parameters for the latter included lenght of epithelial tissue (ET), new attachment (NACT) and connective tissue aposition (CTA), new cementum (NC), and new bone (NB). Histometric data were converted to percentage and statistically analyzed by ANOVA followed by Tukey test at a p<0.05. No significant difference was detected in the following parameters for both groups: ET yielding... (Complete abstract click electronic access below)
Doutor
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Bonfante, Samara [UNESP]. "Padrão de cicatrização de defeitos de deiscência periodontal tratados com enxerto de tecido conjuntivo subepitelial ou matriz dérmica acelular: estudo histológico e histométrico em cães." Universidade Estadual Paulista (UNESP), 2008. http://hdl.handle.net/11449/104706.
Full textAbstract:
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O objetivo do presente estudo foi avaliar histológica e histometricamente o padrão de cicatrização de defeitos de deiscência periodontal tratados com enxerto de tecido conjuntivo subepitelial (ETC) ou matriz dérmica acelular (MDA), cada qual comparado ao tratamento com Retalho posicionado Coronal (RPC). Foram utilizados 10 cães, divididos em dois grupo de 5. Defeitos de deiscência óssea (6x8 mm) foram criados nos caninos superiores e os grupos divididos seguindo um modelo de boca dividida de acordo com o tratamento. Em um grupo de 5 cães foi realizado o tratamento com ETC e no lado contralateral RPC e o outro grupo de 5 cães foi tratado com MDA e RPC no lado contralateral. Após 3 meses pós-operatórios os animais foram submetidos à eutanásia e os blocos processados para análise histológica e histométrica. Os parâmetros histométricos avaliados incluíram extensão de tecido epitelial (TE), nova inserção (NITC) e aposição de tecido conjuntivo (ATC), novo cemento (NC) e novo osso (NO). Os dados histométricos transformados em porcentagem, foram estatisticamente analisados pelo teste ANOVA, seguido pelo teste de Tukey...
The aim of the present study was to evaluate histologically and histometrically the healing pattern of periodontal dehiscence defects treated with Subepithelial Connective Tissue Graft (CTG) or Acellular Dermal Matrix (ADM), when compared to the Coronally Positioned Flap (CPF). Dehiscence bone defects (6x8mm) were created bilaterally in the maxillary canine area of dogs (n=10, divided in 2 groups). By means of a split mouth test design, a group of 5 dogs was randomly allocated to receive subepithelial connective tissue graft (CTG) in one of the sides and a coronally positioned flap (CPF) in the opposing side. The remaining 5 dogs had acellular dermal matrix graft (ADM) as a treatment in either one of the sides and CPF on the other side. Three months after surgery, dogs were euthanized and blocks of interest were processed. The histometric parameters for the latter included lenght of epithelial tissue (ET), new attachment (NACT) and connective tissue aposition (CTA), new cementum (NC), and new bone (NB). Histometric data were converted to percentage and statistically analyzed by ANOVA followed by Tukey test at a p<0.05. No significant difference was detected in the following parameters for both groups: ET yielding... (Complete abstract click electronic access below)
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Vernon, Lauren Louise. "A Comparison of the Osteogenic Tissue Engineering Potential of Dental-Derived Stem Cell Lines: Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs) vs. Periodontal Ligament Stem Cells (PERIOS)." Scholarly Repository, 2010. http://scholarlyrepository.miami.edu/oa_theses/19.
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The goal of this study is to assess the osteogenic potential of two types of dental stem cell lines within a tissue engineering application. More specifically, the goal of this study is to find a readily abundant cell source with capacity to express an osteogenic phenotype. There are two parameters utilized to evaluate tissue engineering potential of cells: proliferation rate and differentiation potential. Briefly, proliferation rate is the speed at which cells divide and differentiation potential determines if cells are capable of committing towards specific lineages (e.g. osteogenic). These components are important, because if cells are not expanding at a specific rate and are not differentiating towards the lineage desired, the tissue engineered will not mirror the characteristics of native tissue. Therefore, both components are necessary for osteogenic tissue engineering applications. Several stem cell lines have been isolated from different sources (e.g. umbilical, bone marrow) and characterized for their proliferative capacity and their potency. Among these progenitor or stem cell lines, are those isolated from human dental tissue. Due to the similarities between teeth and bone, this specific cell line may be useful in osteogenic tissue engineering applications. In this study, stem cells extracted from human exfoliated deciduous teeth (SHEDs) and periodontal ligament stem cells (PERIOs), were evaluated and compared. Briefly, to evaluate the proliferation rate an ex-vivo expansion study was conducted. This experiment found that both SHEDs and PERIOs were proliferative lines with doubling times of 23 hours and 19 hours respectively. Subsequently, osteogenic differentiation of SHEDs and PERIOs was assessed utilizing a 3-D fibrin gel suspension treated with osteogenic media containing either dexamethasone (DEX) or Retinoic Acid (RA) for 28 days. At day 28, osteogenic markers for collagen 1 (Col1), osteocalcin (OCN), and alkaline phosphatase (ALP) were evaluated using qPCR. Results demonstrated both SHEDs and PERIOs exhibited significant (p<0.05) increases in osteogenic gene expression under the influences of DEX and RA. However the most significant increases were expressed by the SHEDs that received the DEX treatment. Additionally, the synergistic ability of TGF-beta 3 on the osteogenic differentiation of the stem cells was evaluated. Cells were cultured in a 3-D fibrin gel suspension and allowed to differentiate in DEX osteogenic media with and without the supplementation of TGF-beta 3 for 21 days. Using qPCR the cells were evaluated for expression of Col1, OCN, and ALP. In both the SHEDs and PERIOs, the samples treated with TGF-beta 3 the osteogenic gene expression increased in reference to the control, but had a hindering effect compared to cells treated in DEX without the TGF-beta 3. These results from this study suggested, SHED cells grown in 3-D fibrin gel suspension, may be better than PERIO cells for osteogenic tissue engineering applications when treated with DEX media without the supplementation of TGF-beta 3.
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Esper, Luis Augusto [UNESP]. "Influência do plasma rico em plaquetas associado ou não à regeneração tecidual guiada na cicatrização de defeitos de fenestração periodontal em cães: estudo histológico e histométrico." Universidade Estadual Paulista (UNESP), 2009. http://hdl.handle.net/11449/96169.
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esper_la_me_araca.pdf: 415604 bytes, checksum: 36bcb3441620df6465aef668552a719f (MD5)Este estudo avaliou histomorfometricamente o processo de cicatrização em defeitos de fenestração periodontal, criados cirurgicamente em cães e tratados com Plasma Rico em Plaquetas (PRP) associado ou não ao uso de barreira de membrana. Defeitos de fenestração periodontal com 5 mm de diâmetro foram cirurgicamente criados nos caninos superiores de 12 cães. Os dentes foram divididos em 4 grupos: C (controle) - defeito preenchido somente com coágulo sangüíneo; M - defeito preenchido com coágulo sangüíneo e protegido com uma membrana de politetrafluoretileno expandido (PTFE-e) com reforço de titânio; PRP - defeito preenchido com PRP; PRP/M - defeito preenchido com PRP e protegido com uma membrana de PTFE-e com reforço de titânio. Os animais foram eutanasiados após 4 semanas. Medidas lineares e de área da cicatrização periodontal foram avaliadas e calculadas como porcentagem do defeito original. Os dados foram submetidos à análise estatística (análise de variância, p < 0,05). Nenhum espécime regenerou-se completamente com osso ou cemento. Formação de novo cemento foi significativamente maior nos Grupos PRP e PRP/M quando comparados ao Grupo C. Observou-se, também, significativa maior formação de novo cemento no Grupo PRP/M que no Grupo M. Dentro dos limites deste estudo, pode-se concluir que o PRP favoreceu a formação de novo cemento. A Regeneração Tecidual Guiada (RTG) não proporcionou efeitos adicionais ao uso do PRP no tratamento de defeitos de fenestração periodontal em cães.
This study histomorphometrically analyzed the healing of periodontal fenestration defects surgically created in dogs and treated with Platelet-Rich Plasma (PRP) with or without a barrier membrane. A 5 mm diameter periodontal fenestration defect was made in each upper canine of 12 dogs. The teeth were divided into 4 groups: C (control) - defect filled with blood clot only; M - defect filled with blood clot and covered by a titanium-reinforced expanded polytetrafluoroethylene membrane (ePTFE); PRP - defect filled with PRP; PRP/M - defect filled with PRP and covered by a titanium-reinforced ePTFE. All animals were euthanized at 4 weeks post-operative. Linear and area measurements of periodontal healing were evaluated and calculated as a percentage of the original defect. Data were statistically analyzed (analysis of variance, p < 0.05). No defect completely regenerated with either bone or cementum. Cementum formation was significantly greater in groups PRP and PRP/M when both groups were compared to Group C. A significant greater cementum formation was also observed in Group PRP/M than in Group M. Within the limits of this study, it can be concluded that the PRP favored cementum formation. Guided Tissue Regeneration (GTR) did not promote any additional benefit to the use of PRP in the treatment of periodontal fenestration defects in dogs.
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Queiroz, Adriana Corrêa de. "Avaliação do uso de matriz óssea bovina inorgânica associada ao peptídeo de adesão celular no tratamento de defeitos infra-ósseos em pacientes com periodontite agressiva. Estudo clínico, radiográfico e laboratorial em humanos." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/58/58132/tde-20022009-104827/.
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Introdução: As periodontites agressivas (PAg) compõem um grupo de formas rapidamente progressivas da doença periodontal. A restauração do periodonto é um objetivo da terapia periodontal, sendo a regeneração tecidual guiada (RTG) e o uso de substitutos ósseos técnicas bem documentadas. Em pesquisas recentes, foi demonstrado o envolvimento de uma cadeia de 15 aminoácidos do colágeno (P-15) na diferenciação celular de fibroblastos e osteoblastos. A associação de matriz óssea inorgânica bovina com o P-15 (MOI/P-15) tem apresentado bons resultados. O objetivo dessa pesquisa foi avaliar a eficácia da MOI/P-15 no tratamento de defeitos periodontais infra-ósseos em pacientes com periodontite agressiva, tendo como controle o uso de membrana de PTFEe com reforço de titânio Metodologia: Foram selecionados 15 pacientes com PAg, com pelo menos dois defeitos periodontais infra-ósseos (profundidade de sondagem (PS)≥4mm e componente infra-ósseo≥3mm). Foi adotado o modelo boca dividida, sendo realizadas cirurgias regenerativas com MOI/P-15 (GT) de um lado e membrana de PTFEe (GC) do outro. As medidas clínicas de PS, nível de inserção relativo (NIR) e recessão gengival (RG) foram registradas no exame inicial e após 6 meses. Exames radiográficos padronizados foram feitos no exame inicial e após 3 e 6 meses e radiografias de subtração foram realizadas. Medidas lineares e de área foram registradas. Foram colhidas amostras do fluido gengival antes da cirurgia e aos 3 e 6 meses pós-operatórios e a presença de interleucina 1 beta (IL-1β) e interleucina 6 (IL-6) foi quantificada através de ensaio imunoenzimático. Resultados: Houve redução significativa na PS, de 2,27±0,96 mm (P<0,001) para o GT e de 2,57±1,06 mm para o GC (P<0,001); aumento significativo no NIR, de 1,87±0,94 mm (P<0,001) para o GT e 2,09±0,88 mm (P<0,001) para o GC; e aumento significativo na RG, de 0,58±0,29 mm (P<0,001) para GT e 0,64±0,47 mm (P<0,001) para o GC. Não foram observadas diferenças estatisticamente significantes entre os grupos em nenhum dos parâmetros, tanto no exame inicial quanto após 6 meses. Na análise radiográfica, as radiografias de subtração apresentaram ganho médio de área radiopaca em relação ao defeito inicial de 93,16% para o GT, contra 62,03% para o GC. O preenchimento radiográfico do defeito foi maior (P=0,002). para GT (2,49 mm) que para GC (0.,73 mm). Houve um aumento progressivo da densidade radiográfica para ambos os grupos, sem diferenças estatisticamente significantes. Na análise das citocinas, não foram observadas diferenças estatisticamente significantes nas comparações intra e entre os grupos. Conclusão: No tratamento de defeitos infra-ósseos em pacientes com PAg-G, em um período de 6 meses, não foram observadas diferenças significantes entre as duas modalidades terapêuticas avaliadas (MOI/P-15 e RTG) quanto aos parâmetros clínicos e quantificação de citocinas. GT apresentou preenchimento radiográfico do defeito superior ao apresentado por GC.Background: Intrabony periodontal defects present a particular treatment problem, especially in patients with Generalized Aggressive Periodontitis (G-AgP).Researches have been performed in order to improve the results of regenerative procedures. Material and Methods: The aim of this study was to compare outcomes of intrabony periodontal defects following treatment with anorganic bone matrix/cell binding peptide (ABM/P-15) to guided tissue regeneration (GTR) in patients with GAgP. Fifteen patients, with two infrabony defects ≥3 mm deep, were selected for the present study. Patients were allocated randomly to be treated with ABM/P-15 or GTR. At baseline and at 6 months after surgery, clinical and radiographic parameters and IL-1β and IL-6 gingival fluid concentrations were recorded. Results: There was a significant PD reduction (P<0.001) for both groups (2.27±0.96 mm for ABM/P-15 group and 2.57±1.06 mm for GTR group) A CAL gain (1.87±0.94 mm for ABM/P-15 group and 2.09±0.88 mm for GTR group) was observed. In between-group comparisons, there were no statistical significant differences in clinical parameters. The radiographic bone fill was more expressive in ABM/P-15 group (2.49 mm) than in GTR group (0.73 mm). In subtraction radiographs, the areas representing gain in density were 93.16% of the baseline defect for ABM/P-15 group versus 62.03% in GRT group. There were no statiscally significant differences in between and intra-group comparison with regard to IL-1β and IL-6 quantification. Conclusion: Treatment of infrabony periodontal defects in patients with G-AgP with ABM/P-15 and GTR significantly improved clinical outcomes. The use of ABM/P-15 promoted a better radiographic bone fill.
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Микитенко, Андрій Олегович, Андрей Олегович Микитенко, and Andrii Olehovych Mykytenko. "Патогенетичне обгрунтування ефективності мультипробіотикотерапії у хворих на хронічний генералізований пародонтит (експериментально-клінічне дослідження)." Thesis, СумДУ, 2015. http://essuir.sumdu.edu.ua/handle/123456789/41869.
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Дисертаційна робота присвячена обґрунтуванню патогенетичного лікування хворих на хронічний генералізований пародонтит за допомогою мультипробіотиків. Результати експериментальних досліджень показали, що за умов тривалого введення інгібітора протонної помпи омепразолу виникають патологічні зміни в тканинах пародонта щурів: деполімеризація біомолекул сполучної тканини, зниження активності інгібіторів протеїназ на тлі розвитку оксидативного стресу та змін загальної активності NO-синтази і вмісту нітрит-аніонів. Уведення мультипробіотика за умов тривалого пригнічення секреції гідрохлоридної кислоти в шлунку запобігає розвитку оксидативного стресу, катаболізму колагенових і неколагенових білків тканин пародонта, сприяє підвищенню загальної активності NO-синтази та вмісту нітрит-аніонів у порівнянні з тваринами без корекції. Доведена клінічна ефективність мультипробіотикотерапії у хворих на хронічний генералізований пародонтит І-ІІ ступенів тяжкості на підставі клінічних, біохімічних і мікробіологічних досліджень.Диссертационная работа посвящена изучению роли дисбиотических нарушений в патогенезе развития заболеваний пародонта и возможности коррекции их мультипробиотиками. Современный фармацевтический рынок имеет широкий спектр пробиотических препаратов, большинство из которых являются монокомпонентными в лиофилизированном состоянии, что противопоказано для использования в стоматологической практике, так как реактивация клеток происходит в проксимальных отделах пищеварительного тракта. Отечественный мультиштаммный пробиотик «Симбитер омега» имеет много преимуществ: 1. живые культуры; 2. мультиштаммность за счет мутуалистичного симбиоза; 3. высокая концентрация живых культур в одной дозе 2*1010 КОЕ; 4. 200 мг высокоочищенного геля бентонита, основой которого является минерал монтмориллонит; 5. 250 мл масла льна и пшеницы, которые являются источником ω-3 і ω-6 полиеновых жирных кислот. Считаем, что мультипробиотическая концепция коррекции микробиоценоза полости рта имеет целью стойкое восстановление физиологического дисбаланса микробиологических и иммунологических звеньев колонизационной резистентности с учетом особенностей данной микробиоты. В эксперименте на животных проанализированы интенсивность свободнорадикальных процессов в тканях пародонта, степень деполимеризации коллагеновых белков, протеогликанов и гликопротеидов, изменения NO-системы и активности ингибиторов протеолитических ферментов в условиях 28-дневного введения ингибитора протонной помпы омепразола. Экспериментальная коррекция мультипробиотиком патологических изменений в тканях пародонта крыс в условиях длительного введения омепразола доказала пародонтопротекторное действие, о чем свидетельствуют предупреждение катаболизма биомолекул соединительной ткани, угнетение развития оксидативного стресса, повышение активности NO-синтазы и содержания нитрит-анионов. Доказано, что использование мультипробиотиков группы «Симбитер» в индивидуальных дентоальвеолярных каппах на ночь в течение 20 дней у больных хроническим генерализованным пародонтитом восстанавливало окислительно-антиоксидантный баланс ротовой жидкости, о чем свидетельствует достоверное снижение содержания окислительно-модифицированных белков, ТБК-реактантов на фоне повышения активности супероксиддисмутазы и каталазы по сравнению с этими показателями у пациентов до лечения. Под действием мультиштаммных пробиотиков группы «Симбитер» в ротовой жидкости больных хроническим генерализованным пародонтитом нормализуются протеиназно-ингибиторный баланс, NO-эргическая система и повышается активность орнитиндекарбоксилазы в сравнении с этими показателями до лечения. Доказано, что мультипробиотикотерапия у больных хроническим генерализованным пародонтитом нормализует микробиоценоз пародонтальных карманов, о чем свидетельствуют микробиологические исследования.
The dissertation is devoted to the substantiation of pathogenetic treatment of patients with chronic generalized periodontitis using multiprobiotics. The results of experimental studies have shown that under conditions of prolonged administration of proton pump inhibitor omeprazole, we have pathological changes in periodontal tissues of rats: depolymerization of biomolecules of connective tissue, reducing in the activity of protease inhibitors against the backdrop of oxidative stress and changes in the overall activity of NO-synthase and the content of nitrite anions. Introduction of multiprobiotic under prolonged inhibition of secretion of hydrochloric acid in the stomach prevents the development of oxidative stress and catabolism of collagen fibers non-collagenic periodontal tissues and increase the total activity of NO-synthase and the content of nitrite anion compared with animals without correction. Multyprobiotic therapy usage showed proven clinical efficiency in patients with chronic generalized periodontitis of I and II degree of severity based on clinical, biochemical and microbiological studies.
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Girelli, Junior Claudio. "Avaliação dos efeitos da deficiência de testosterona sobre os tecidos periodontais de ratos castrados e com periodontite experimental." Universidade Estadual do Oeste do Parana, 2015. http://tede.unioeste.br:8080/tede/handle/tede/739.
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Testosterone is the principal male sex steroid hormone involved in differentiation, sexual development and control of male reproductive functions. In addition to its role in reproductive endocrinology and fertility, testosterone is an important hormone in the regulation and functioning of other organs and tissues including the kidney, heart, skeletal muscle, immune system and oral and periodontal tissues. Periodontitis is a disease characterized by inflammation of the periodontal tissues and associated with dental decay, are mainly responsible for the loss of dental elements. The relationship between sex steroids hormones and periodontal disease has been intensively investigated in females, however studies in males are still scarce. Therefore, the aim of the present project is to analyze the influence of testosterone deficiency on bone loss and histological structure of the periodontal tissues of castrated rats and experimental periodontitis. Twenty-eight male Wistar rats, provided by the Central Biotherium of Unioeste were used. At 80 days of age the animals were separated in four experimental groups, with 7 animals per group: no ligature control (CON), ligature control CON + LIG), no ligature castrated (CAST) and ligature castrated (CAST + LIG). At 90 days of age was held orchiectomy in appropriate groups. Sixty days after castration, the periodontal disease was induced by ligature technique. At the end of experimental period (90 days after castration), the animals were weighed and euthanized in a CO2 chamber. The mandible was removed, dissected, separated into left and right, were fixed by immersion in 10% buffered formalin for 24 hours, decalcified and processed for histological and radiological techniques. The results of this study showed that ligature model was effective 13 in inducing periodontitis in the animals. The food intake profile was similar between groups. The animals of CAST and CAST + LIG groups showed significant reduction in body weight at the end of experimental period, when compared with the groups CON and CON + LIG. Castration resulted in a significant bone loss in animals, which was accentuated with the induction of periodontal disease. Animals with periodontal diseases presented increased of gingival epithelium and connective tissue area, compared to animals without the disease. We conclude that testosterone is a physiological regulator important of alveolar bone metabolism. Testosterone deficiency in synergism with periodontal disease increases alveolar bone resorption and alters the thickness of gingival epithelium.Key
A testosterona é o principal hormônio sexual esteróide masculino envolvido na diferenciação, desenvolvimento sexual e controle das funções reprodutivas masculina. Além do seu papel na endocrinologia reprodutiva e fertilidade, a testosterona é um hormônio importante na regulação e funcionamento de outros órgãos e tecidos corporais tais como o rim, coração, músculo estriado esquelético, sistema imunológico e os tecidos orais e periodontais. A periodontite é uma doença caracterizada pelo processo inflamatório dos tecidos periodontais e juntamente com a cárie, são as grandes responsáveis pela perda de elementos dentais. As relações entre os hormônios sexuais esteróides e doença periodontal tem sido intensamente investigado em fêmeas, entretanto os estudos em machos ainda são escassos. Assim, o presente trabalho teve por objetivos analisar a influência da deficiência de testosterona sobre a perda óssea alveolar e estrutura histológica dos tecidos periodontais de ratos castrados e com periodontite experimental. Foram utilizados 28 ratos machos Wistar, fornecidos pelo Biotério Central da Unioeste. Aos 80 dias de idade os animais foram separados em quatro grupos experimentais, com 7 animais por grupo: controle sem ligadura (CON), Controle com ligadura (CON+LIG), castrado sem ligadura (CAST) e castrado com ligadura (CAST+LIG). Aos 90 dias de idade realizou-se a orquidectomia nos devidos grupos. Sessenta dias após a castração a doença periodontal foi induzida através da técnica de ligadura. Ao final do período experimental (90 dias após a castração), os animais foram pesados e sacrificados em câmara de CO2. As mandíbulas foram retiradas, dissecadas, separadas em direita e esquerda, fixada em formalina tamponada a 10% por 24 horas, descalcificada e 11 processadas para técnicas histológicas e radiológicas. Os resultados do presente trabalho demonstraram que o modelo de ligadura foi eficiente em induzir a periodontite nos animais. O perfil de consumo alimentar foi semelhante entre os grupos. Os animais dos grupos CAST e CAST+LIG apresentaram significativa redução do peso corporal ao final do período experimental, quando comparados aos grupos CON e CON+LIG. A castração levou a uma significativa perda óssea nos animais, a qual foi acentuada com a indução da doença periodontal. Os animais com doença periodontal apresentaram aumento de área do epitélio gengival e área de tecido conjuntivo, quando comparado aos animais sem a doença. Concluímos que a testosterona é um importante regulador fisiológico do metabolismo ósseo alveolar. A deficiência de testosterona em sinergismo com a doença periodontal aumenta a reabsorção óssea alveolar e altera a espessura do epitélio gengival.
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Zhuang, Longfei, and 庄龙飞. "Periodontal and peri-implant microbiota in subjects with healthy and inflamed tissues." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/206427.
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Bacteria, in the form of biofilm, has been shown to play a critical role in the etiology and pathogenesis of periodontal and peri-implant infectious diseases. Studies have shown that distinctively different dental plaque is commonly found in healthy versus inflamed gingivae and mucosa. It should be noted, however, that in most of these studies, the healthy and diseased plaque samples were collected from different individuals. To address this important issue, in the two studies described within this thesis, I recruited subjects who were periodontally involved and/or had inflamed peri-implant tissues, and also had equivalent healthy control sites.
In the first study, I analysed the subgingival microbiota of a cohort of tea labourers from Sri Lanka, who had never performed any oral hygiene practices. Within each of the 32 subjects, one ‘shallow’ (healthy) site and one ‘deep’ (diseased) site were chosen for subgingival plaque sampling. A 16S ribosomal RNA (16S rRNA) gene sequencing method was applied to investigate the diversity of the subgingival microbiome, and to compare the microbial composition between healthy and diseased sites. A taxonomically diverse subgingival microbiota was identified, with 318 operational taxonomic units (OTUs; 98% identity cut-off) from 1,887 cloned full-length 16S rRNA gene sequences. The subgingival microbiota was dominated by the phyla Firmicutes, Proteobacteria and Fusobacteria. A significant difference in the overall composition of microbial communities between shallow and deep sites was found ((-Libshuff, p<0.001) while pairwise comparisons within each subject revealed no significant differences. The absence of oral hygiene resulted in a highly diverse subgingival microbiota in this cohort.
In the second study, 22 subjects who had both implants and teeth surrounded by healthy and inflamed tissues, were included for subgingival/submucosal microbiological sampling. Quantitative real-time polymerase chain reaction (q-PCR) was used to detect and to quantify six species, including putative periodontal pathogens, i.e., Porphyromonas gingivalis (P.g.), Treponema denticola (T.d.), Aggregatibacter actinomycetemcomitans (A.a.), Fusobacterium nucleatum (F.n.), Prevotella intermedia (P.i.), and Staphylococcus aureus (S.a.). Within the same subjects, putative periodontal pathogens were common to both periodontal and peri-implant sites irrespective of health status. The detection frequencies for each of the six target species at diseased tooth or implant sites were either equal to, or higher than, the respective detection frequencies at the corresponding healthy sites. Both periodontal and peri-implant sites, irrespective of their health status, were revealed to harbour S. aureus. Even though the target organisms were found in all clinical conditions, there were differences in the involvement of some of the pathogens for the diseased conditions. The prevalence and levels of P. gingivalis and F. nucleatum were significantly associated with periodontitis, but not with peri-implantitis. A. actinomycetemcomitans was associated with both disease conditions, periodontitis and peri-implantitis, but not with either gingival or mucosal health.
In conclusion, results from my two studies indicated that the differences between the composition of subgingival microbial communities present in single sites within two different individuals, were always greater than the differences in microbial community composition present in two subgingival sites of differing health status within the same individual.published_or_final_version
Dentistry
Doctoral
Doctor of Philosophy
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Ноncharenko, V. A. "Structure of periodontic tissue diseases in children with insulin dependence diabetes mellitus." Thesis, БДМУ, 2021. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/19123.
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Карпуша, Т. В. "Вплив солей важких металів на розвиток стоматологічної патології." Thesis, Буковинський державний медичний університет, 2017. http://essuir.sumdu.edu.ua/handle/123456789/51759.
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Солі важких металів мають значний негативний вплив на всі системи організму і на зубо-щелепну систему безпосередньо. При дії їх комбінації спостерігаються дистрофічні та деструктивні зміни у кістковій тканині. При реадаптації спостерігається відновлення ультраструктури кісткових клітин, проте повної нормалізації не відмічається. Тривале надходження в організм надлишку солей важких металів може спричиняти підвищення інтенсивності карієсу зубів, а також підвищення поширеності і інтенсивності ураження тканин пародонту. Надлишок солей важких металів у довкіллі впливає на поширеність зубощелепних аномалій.Соли тяжелых металлов имеют значительное негативное влияние на все системы организма и на зубо-челюстную систему. При действии их комбинации наблюдаются дистрофические и деструктивные изменения в костной ткани. При реадаптации наблюдается восстановление ультраструктуры костных клеток, однако полной нормализации не отмечается. Длительное поступление в организм избытка солей тяжелых металлов может вызвать повышение интенсивности кариеса зубов, а также повышение распространенности и интенсивности поражения тканей пародонта. Избыток солей тяжелых металлов в окружающей среде влияет на распространенность зубочелюстных аномалий.
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MAISTRO, ADAIANA M. "Monitoramento da doenca periodontal em modelo animal pelo sistema de tomografia por coerencia optica." reponame:Repositório Institucional do IPEN, 2010. http://repositorio.ipen.br:8080/xmlui/handle/123456789/9596.
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Dissertacao (Mestrado Profissionalizante em Lasers em Odontologia)
IPEN/D-MPLO
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP; Faculdade de Odontologia, Universidade de Sao Paulo, Sao Paulo
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Silva, Robert Carvalho da. "Avaliação clinica do tratamento de recessões gengivais." [s.n.], 2002. http://repositorio.unicamp.br/jspui/handle/REPOSIP/290368.
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Orientador: Antonio Fernando Martorelli de LimaDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba
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Resumo: Este estudo avaliou o resultado do tratamento de recessões gengivais usando o retalho colocado coronal associado ou não ao enxerto subepitelial de tecido conjuntivo, grupos teste e controle, respectivamente. Os índices dicotômicos de Placa (IPI) e Gengival (IG), e os parâmetros lineares Profundidade de Sondagem (PS), Nível da Margem Gengival (NMG), Nível Clínico de Inserção (NCI), Faixa de Tecido Queratinizado (TQ) e espessura gengival (Esp 1 e 2) foram obtidos imediatamente antes do procedimento cirúrgico e 6 meses após. Os índices de Placa (IPI) e de Sangramento (IS) foram mantidos abaixo de 20% durante todo o período experimental. Os dados referentes aos parâmetros lineares foram avaliados pelo teste t pareado de Student para comparação entre os tempos e entre os grupos experimentais. Não houve diferença estatística significativa entre os grupos para nenhuma das variáveis no exame inicial (p > 0,05). No grupo teste, houve diferença estatística significativa (p < 0,05) para todos os parâmetros entre os tempos experimentais, enquanto que, no grupo controle, a diferença estatística significativa foi encontrada apenas nos parâmetros PS, NMG e NCI (p < 0,05). A comparação entre os grupos 6 meses após os procedimentos de recobrimento radicular mostrou diferença estatística entre os parâmetros TQ, Esp 1 e Esp 2 (p <0,05), entretanto não houve diferença (p > 0,05) nos parâmetros PS, NMG e NCI. Ambas técnicas cirúrgicas foram eficientes para produzir recobrimento radicular. A aplicação clínica do retalho colocado coronal associado ao enxerto subepitelial de tecido conjuntivo foi mostrada em três situações clínicas diferentes associadas a problemas estéticos e funcionais. Foram utilizadas diferentes formas de avanço coronário do retalho sobre o enxerto de tecido conjuntivo subepitelial que proporcionaram recobrimento completo da superfície radicular exposta com aumento significativo da faixa de gengiva queratinizada e espessura gengiva!. Esses resultados confirmaram que a associação do retalho colocado coronal e o enxerto de tecido conjuntivo subepitelial foi eficiente na resolução de problemas estéticos e funcionais
Abstract: This study evaluated the results of the treatment of gingival recessions using the coronally positioned flap associated or not with the subepithelial connective tissue graft, test group and control group, respectively. The dichotomic Plaque Index (PU) and Gingival Index (GI), as well as the linear parameters Probing Depth (PD), Gingival Margin Levei (GML), Clinical Attachment Level (CAL), width of keratinized Tissue (KT) and Gingival/mucosal Thickness (GT1 and GT2) were assessed at baseline and 6 months latter. 80th Plaque Index (PU)and Gingivallndex (GI) were maintained below 20% through all the experimental period. Data corresponding to the linear parameters were analyzed using Student t test for paired observations to assess changes obtained within and between groups. The results did not show significant statistical differences between groups for any of the parameters at baseline evaluation (p > 0.05). In the test group, there was significant statistical difference for all parameters between the evaluation periods (p < 0.05), however, in the control group, significant difference was found for PD, GML and CAL only (p<0,05). The between-groups comparison 6 months postsurgery showed significant difference for KT, GT1 and GT2 (p < 0,05), however no significant difference was found for PD, GML and CAL (p > 0,05). 80th approaches were effective to produce root coverage. The clinical application of the coronally positioned flap associated with the subepithelial connective tissue graft was shown in three different clinical situations associated with aesthetic and functional problems. Different forms of coronally advancing the flap over the subepithelial connective tissue graft were used which provided complete root coverage and significant increase of keratinized tissue width as well as gingival thickness. These results confirmed that the coronally positioned flap was effective in the resolution of aesthetic and functional problems
Mestrado
Periodontia
Mestre em Clínica Odontológica
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CARGNELUTTI, TATIANA. "Estudo comparativo da influencia da terapia fotodinamica antimicrobiana e remocao mecanica de biofilme no processo inflamatorio periodontal induzido em hamsters." reponame:Repositório Institucional do IPEN, 2007. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11511.
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Dissertacao (Mestrado Profissionalizante em Lasers em Odontologia)
IPEN/D-MPLO
Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP
49
Batig, V. M. "Influence of oral applications of small doses of adrenaline on the biochemical indicators of periodontal tissues of rats." Thesis, БДМУ, 2020. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/17834.
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Sukekava, Flávia. "Características da neo formação dos tecidos moles periimplantares e periodontais após excisão cirúrgica de margem." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/23/23146/tde-13042009-104633/.
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O presente estudo descreve as características da cicatrização de tecido mole ao redor de dentes e implantes, em 5 cães beagle, após remoção cirúrgica. Os terceiros e quartos pré-molares inferiores bilateralmente foram extraídos. Três meses após, 2 implantes de titânio Osseospeed foram instalados em cada quadrante inferior, juntamente com a instalação imediata do conector na região dos pré-molares extraídos previamente. Três meses após a instalação dos implantes, quatro áreas contendo um implante e um dente foram determinadas e identificadas em cada cão. Uma área foi aleatoriamente escolhida em cada animal para receber a cirurgia ressectiva na face vestibular do dente e do implante. Na face lingual não foi executado qualquer procedimento ficando esta face como lado controle. As 3 áreas remanescentes foram tratadas de maneira idêntica e os animais foram ortotanasiados e forneceram biópsias representando os seguintes intervalos de cicatrização 1, 2, 4 e 12 semanas. As biópsias foram preparadas para análise histological e morfométrica. Foi observado que em 12 semanas a altura da mucosa periimplantar e da gengiva foram similares ao lado controle. Além disso, a análise morfométrica demonstrou que no intervalo 12 semanas o volume de feixes de fibras colágenas, fibroblastos, estruturas vasculares e tecido residual não foi similar entre o grupo de implantes comparando grupo teste com grupo controle. O presente experimento demonstrou que a cicatrização do tecido mole ao redor de implantes parece ser mais lenta que ao redor de dentes.The current presentation will describe a study in the dog features characteristic of soft tissue healing after ressective surgery at teeth and implants. Five beagle dogs were used. In both quadrants of the mandible, the third and fourth premolars were extracted. Three months later, two titanium fixtures Osseospeed were installed and abutment connection performed in each mandibular quadrant at the premolar region of the edentulous ridge. Following additional 3 months of healing, four regions containing each implant and an adjacent tooth were identified in each dog. One region was randomly selected and soft tissue ressective surgery was performed at the buccal aspect. The lingual soft tissues were not included in the experimental surgical procedure and were regarded as control sites. The remaining three regions were treated in an identical manner and the dogs sacrificed to provide biopsies representing healing intervals of 1, 2, 4 and 12 weeks. The biopsies were prepared for histological and morphological analyses. It was observed that at the 12-weeek interval the height of the soft tissue both at the gingival and implant mucosa was similar to the one at the control site. Furthermore, the morphometric analyses demonstrated that the 12-week interval the volume of collagen fibers, fibroblasts, vessels and residual tissue in the healing tissues was not similar between the gingival and periimplant mucosa also differed from the control sites regarding the vascular content. The present experiment demonstrated that soft tissue healing around implants seems to be slower than around tooth.