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1
Cloyd, Raymond A. "Getting Mixed-Up: Are Greenhouse Producers Adopting Appropriate Pesticide Mixtures to Manage Arthropod Pests?" HortTechnology 19, no. 3 (2009): 638–46. http://dx.doi.org/10.21273/hortsci.19.3.638.
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Pesticide mixtures are commonly used by greenhouse producers to deal with the array of arthropod (insect and mite) pests encountered in greenhouses. Greenhouse producers tank mix pesticides due to convenience because it is less time consuming, costly, and labor intensive to mix together two or more pesticides into a single spray solution and then perform one spray application compared with making multiple applications. Pesticide mixtures may also result in improved arthropod pest control. However, there has been no quantitative assessment to determine what pesticide mixtures (two-, three-, and four-way combinations) are being adopted by greenhouse producers and why. As such, a survey was conducted by distributing evaluation forms in conjunction with three sessions at two greenhouse producer conferences (two in 2007 and one in 2008) to obtain data on the types of pesticide mixtures used by greenhouse producers and determine if there are any problems associated with these pesticide mixtures. The evaluation form requested that participants provide information on the four most common pesticide mixtures (insecticides and/or miticides) used and for what specific arthropod pests. The response rate of the evaluation forms was 22.5% (45/200). The two-way pesticide mixture that was cited most often (n = 8) was the abamectin (Avid) and bifenthrin (Talstar) combination. The two pesticides typically included in a majority of the two-way and three-way mixtures were spinosad (Conserve) and abamectin. Spinosad was a component of 17 two-way and 7 three-way combinations, while abamectin was cited in 15 two-way and 9 three-way combinations. Both products are labeled for control of the western flower thrips (Frankliniella occidentalis), which is one of the most important insect pests in greenhouses. One pesticide mixture that was difficult to interpret involved the fungicides, thiophanate-methyl (Cleary's 3336) and metalaxyl (Subdue). This mixture was cited twice, and the arthropod pest listed was thrips (Thysanoptera). However, both fungicides have no insecticidal activity. Two of the mixtures listed in the survey used pesticides with similar modes of action: acephate (Orthene) + methiocarb (Mesurol), and pyrethrins (Pyreth-It) + bifenthrin (Talstar). A number of the pesticide mixtures listed for spider mites (Tetranychidae) were questionable due to similar life stage activity of the a.i. as indicated on the label including fenpyroximate (Akari) + clofentezine (Ovation), abamectin + chlorfenapyr (Pylon), and bifenazate (Floramite) + etoxazole (TetraSan). In fact, 38% of pesticide mixtures cited for twospotted spider mite (Tetranychus urticae) control should have been avoided due to analogous life stage activity. The data obtained from the survey clearly demonstrates that greenhouse producers implement a wide-range of pesticide mixtures to deal with the multitude of arthropod pests in greenhouses. However, the basis by which greenhouse producers decide the types of pesticides to mix together is not known. As such, the survey data can be used to direct future multistate or multiregional extension (outreach) efforts in developing programs specifically designed to educate greenhouse producers on which pesticides should and should not be mixed together.
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Weeks Santos, Shannon, Jérôme Cachot, Bettie Cormier, et al. "Environmentally Relevant Mixture of Pesticides Affect Mobility and DNA Integrity of Early Life Stages of Rainbow Trout (Oncorhynchus mykiss)." Toxics 9, no. 8 (2021): 174. http://dx.doi.org/10.3390/toxics9080174.
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The aim of this study was to analyze the impact of three concentrations of a pesticide mixture on the first development stages of rainbow trout (Oncorhynchus mykiss). The mixture was made up of three commonly used pesticides in viticulture: glyphosate (GLY), chlorpyrifos (CPF) and copper sulfate (Cu). Eyed stage embryos were exposed for 3 weeks to three concentrations of the pesticide mixture. Lethal and sub-lethal effects were assessed through a number of phenotypic and molecular endpoints including survival, hatching delay, hatching success, biometry, swimming activity, DNA damage (Comet assay), lipid peroxidation (TBARS), protein carbonyl content and gene expression. Ten target genes involved in antioxidant defenses, DNA repair, mitochondrial metabolism and apoptosis were analyzed using real-time RT-qPCR. No significant increase of mortality, half-hatch, growth defects, TBARS and protein carbonyl contents were observed whatever the pesticide mixture concentration. In contrast, DNA damage and swimming activity were significantly more elevated at the highest pesticide mixture concentration. Gene transcription was up-regulated for genes involved in detoxification (gst and mt1), DNA repair (ogg1), mitochondrial metabolism (cox1 and 12S), and cholinergic system (ache). This study highlighted the induction of adaptive molecular and behavioral responses of rainbow trout larvae when exposed to environmentally realistic concentrations of a mixture of pesticides.
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Warnock, Daniel F., and Heather Lash. "Tank Mixtures Differentially Impact Survival of Predatory Mites used to Manage Western Flower Thrips." HortScience 40, no. 4 (2005): 1125C—1125. http://dx.doi.org/10.21273/hortsci.40.4.1125c.
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Greenhouses contain a vast array of insect, mite, and disease pests primarily managed by applications of conventional and biorational pesticides including insecticides, miticides, and fungicides. However, biorational pesticides have a narrow range of pest activity. As a result, greenhouse producers tank mix to broaden application activity. Research has demonstrated that tank mixing can result in either synergistic or antagonistic interactions for targeted pests. However, the impact of tank mixing insecticides and fungicides on predatory mites, Neoseiulus cucumeris, used to manage western flower thrips, Franklinella occidentalis, is unknown. The objective of this research was to determine how mixtures of four different pesticides (Conserve, Avid, Cleary's, and Decree), alone and in all possible combinations affect predatory mite survival in a laboratory bioassay. Individual 2-day-old adult mites, isolated in a cell of a bioassay tray, were exposed to one of the 15 pesticide treatments, or a water control. Treatments were replicated 15 times. Trays were held in an environmental chamber and mite mortality was assessed after 24 hours. Mite mortality was differentially impacted by some pesticide treatments when compared with the water control. One pesticide mixture, Conserve + Cleary's, significantly reduced mite survival compared to other pesticide treatments or the water control. Up to 70% of the mites exposed to this treatment died. The combination of Conserve + Cleary's should be avoided as a tank mixture when the biological control agent, Neoseiulus cucumeris, is used to manage western flower thrips.
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Gyorkos, J., M. A. Denomme, B. Leece, K. Homonko, V. E. Valli, and S. Safe. "Reconstituted halogenated hydrocarbon pesticide and pollutant mixtures found in human tissues: effects on the immature male Wistar rat after short-term exposure." Canadian Journal of Physiology and Pharmacology 63, no. 1 (1985): 36–43. http://dx.doi.org/10.1139/y85-006.
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Halogenated hydrocarbon insecticides and polychlorinated biphenyl (PCB) mixtures are routinely detected as residues in human adipose tissue, serum, and milk. Based on average values observed in analytical studies, reconstituted halogenated hydrocarbon pesticide mixtures and PCB mixtures were prepared and administered to immature male Wistar rats. The mixtures were administered at dose levels which approximate the concentrations which would be absorbed by an infant suckling for 180 days (low dose, L), and at three higher dose levels (2 × L, 10 × L, and 100 × L). The pesticide mixture contained isomeric hexachlorocyclohexanes, dieldrin, heptachlor epoxide, oxychlordane, trans-nonachlor, hexachlorobenzene, 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane, 1,1-dichloro-2,2-bis(p-chlorophenyl)ethane, and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene; the reconstituted PCB mixture contained 13 of the major congeners which have been identified in human milk samples. Administration of the L dose level of the pesticide (0.95 mg/kg), PCB (0.45 mg/kg), and pesticide plus PCB mixture (0.95 + 0.45 mg/kg, respectively) in corn oil on days 1 and 3 did not significantly alter hepatic drug-metabolizing enzyme activities or elicit any observable pathological damage 6 days after the first exposure. In contrast, administration of the higher dose levels of this mixture elicited a dose-dependent induction of several hepatic drug-metabolizing enzymes. Moreover, despite the short duration of exposure to these chemicals, the rats treated with the higher doses (10 × L and 100 × L) of these mixtures exhibited mild alterations in thyroid architecture, changes in hepatocellular nuclei including variations in chromatin distribution, vesiculation of larger nuclei, and frequent appearance of pyknotic shrunken nuclei. In addition there were changes in hepatocellular cytoplasm organization including vacuolations and mild zonal variations in volume. The results clearly indicate that relatively low doses of the pesticides and pollutant mixtures commonly found in human breast milk can elicit significant biologic and toxic effects in the immature male Wistar rat.
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Warnock, Daniel F., and Raymond A. Cloyd. "Effect of Pesticide Mixtures in Controlling Western Flower Thrips (Thysanoptera: Thripidae)." Journal of Entomological Science 40, no. 1 (2005): 54–66. http://dx.doi.org/10.18474/0749-8004-40.1.54.
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Greenhouse managers mix together pesticides to broaden the spectrum of pest control and to reduce pesticide and labor costs. However, the efficacy of pesticide mixtures has not been well-documented. This study assesses how mixtures of commercially available insecticides and miticides in two, three, and four-way combinations affected the control of western flower thrips, Frankliniella occidentalis Pergande, in greenhouse experiments and in a laboratory bioassay. The pesticides screened in the greenhouse experiments and laboratory bioassay were spinosad, abamectin, bifenazate, azadirachtin and imidacloprid. Each pesticide was applied at the maximum label-recommended rate. In the greenhouse experiments, transvaal daisy (Gerbera jamesonii H. Bolus ex Hook. f) and lisianthus (Eustoma grandiflorum G. Don ex Sweet) flowers were infested with 25 adult western flower thrips (mixture of females and males). Flowers were then sprayed with the designated treatments. After 72 h, flowers were dissected to assess the numbers of live and dead western flower thrips. In the laboratory bioassay, chrysanthemum (Dendranthema grandiflora Tzvelev) leaf sections, treated with solutions of each pesticide and all possible combinations, were exposed to 15 adult western flower thrips. The numbers of live and dead western flower thrips were assessed after 48 h. For all three experiments, we were not able to identify any antagonistic pesticide combinations. All treatments with spinosad, including the individual application and pesticide mixtures, resulted in high mortality of western flower thrips. Our data suggest that combinations of spinosad with the other pesticides tested do not affect the efficacy of spinosad in controlling western flower thrips. This information is important to greenhouse managers who wish to mix pesticides and still control western flower thrips in addition to the other plant-feeding arthropods found in greenhouses.
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Sadia, Christabelle G., France-Paraudie A. Kouadio, Behi K. Fodjo, et al. "Short-term metabolic resistance inductive effect of different agrochemical groups on Anopheles gambiae mosquitoes." Wellcome Open Research 6 (June 11, 2021): 147. http://dx.doi.org/10.12688/wellcomeopenres.16876.1.
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Background: In order to assess the impact of the different groups of agricultural pesticides used in Côte d'Ivoire on the increase of mosquitoes resistance to insecticides, the expression profiles of 7 P450 cytochromes and one GSTE2 of Anopheles gambiae involved in mosquito resistance to insecticides were studied. The goal of this study was to determine the effect of short exposure of mosquito larvae to different groups of agricultural pesticides on mosquito resistance. Methods: Three groups of pesticides were selected: (i) agricultural insecticide solutions, (ii) none-insecticide pesticide solutions (a mixture of herbicides and fungicides), and (iii) a mixture of the first two. A fourth non-pesticide solution was used as a control. Four groups of each stage 2 larvae (strain Kisumu, male and female) were exposed to 20% concentrated solution for 24 hours. Susceptibility tests for dichlorodiphenyltrichloroethane (DDT) and Deltamethrin were carried out on adults aged 2-5 days. Quantitative reverse transcription polymerase chain reaction (RT-qPCR) was performed to quantify the expression of eight metabolic genes involved in mosquito resistance to insecticides. Results: Susceptibility to DDT showed a similar increase in the time required to knock down 50% of mosquitoes (kdt50) in l colonies exposed to insecticides and none-insecticides compared to the control colony. As for deltamethrin, kdt50 was higher in the colonies exposed to insecticides and the pesticide mixture compared to the colony exposed to none-insecticides. Of all the genes studied in all colonies, except for CYP6P1 induced only in the colony consisting of the pesticide mixture, no genes were induced. Conclusions: This study confirmed that induction is influenced by the duration, the concentration of the solution and the type of xenobiotic used as an inducer. The overexpression of CYP6P1 confirmed the inductive effect that a short exposure of mosquito larvae to agricultural pesticides could have.
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Karns, Jeffrey S. "Biotechnology for the Treatment of Pesticide Waste." HortScience 31, no. 4 (1996): 699c—699. http://dx.doi.org/10.21273/hortsci.31.4.699c.
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The use of microbes and/or microbial processes for the bioremediation of soils contaminated with pesticides is an idea that has enjoyed considerable interest over the past several years. Many microbes with specific pathways for the degradation of particular pesticides, or classes of pesticide, have been isolated and characterized. Unfortunately, most sites that are heavily contaminated with pesticides contain a mixture of the many different types of pesticides that have been used over the last 5 decades. This complex mixture of compounds may inhibit microbial degradation or may require multiple treatments to assure that all the chemicals are degraded. Treatment of wastes before they contaminate the environment is one way to avoid the problems associated with mixed wastes. We have isolated a number of microorganisms that detoxify insecticides, such as carbaryl of parathion via the action of hydrolase enzymes. These enzymes can be used to treat waste pesticide solutions before disposal. A system was developed for the disposal of one high-volume organophosphate insecticide waste by treatment with parathion hydrolase, followed by ozonation to yield harmless products that were readily degraded by other soil microorganisms. A second method for disposal of this waste involves altering the environmental conditions in the waste to stimulate the growth of microorganisms naturally present in the material utilizing the pesticide as a carbon source. This accomplishes degradation of the material over a 2-week period. Many, if not all, pesticides are degradable to some degree by microorganisms, and this fact can be exploited to provide cost-effective methods for the safe disposal of pesticide wastes.
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Renner, Rebecca. "Pesticide mixture enhances frog abnormalities." Environmental Science & Technology 37, no. 3 (2003): 52A. http://dx.doi.org/10.1021/es032366s.
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Djurovic, Rada, Tijana Djordjevic, Ljiljana Radivojevic, Ljiljana Santric, and Jelena Gajic-Umiljendic. "Multiresidue analysis of pesticides in soil by liquid-solid extraction procedure." Pesticidi i fitomedicina 27, no. 3 (2012): 239–44. http://dx.doi.org/10.2298/pif1203239d.
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A multiresidue method for simultaneous determination of four pesticides (diazinon, acetochlor, aldrine and carbofuran) belonging to different pesticide groups, extracted from soil samples, is described. The method presented is based on liquid-solid extraction (LSE) and determination of pesticides, i.e. the pesticides were extracted by methanol- acetone mixture, purified on florisil column and eluted by ethyl acetate-acetone mixture. Optimization of the main parameters affecting the LSE procedure, such as the choice of purification sorbent, as well as the elution solvent and its volume, were investigated in details and optimized. Also, validation of the proposed method was done. Gas chromatography-mass spectrometry (GC-MS) was used for detection and quantification of the pesticides studied. Relative standard deviation (RSD) and recovery values for multiple analysis of soil samples fortified with 30 ?g/kg of each pesticide were below 8% and higher than 89%, respectively. Limits of detection (LOD) for all the compounds studied were less than 4 ?g/kg.
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Albero, Beatriz, Consuelo Sánchez-Brunete, and José L. Tadeo. "Multiresidue Determination of Pesticides in Honey by Matrix Solid-Phase Dispersion and Gas Chromatography with Electron-Capture Detection." Journal of AOAC INTERNATIONAL 84, no. 4 (2001): 1165–71. http://dx.doi.org/10.1093/jaoac/84.4.1165.
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Abstract A multiresidue method was developed for the determination of 15 pesticides (organochlorines, organophosphorus compounds, pyrethroids, and other acaricides) in various commercial honeys (eucalyptus, lavender, orange, rosemary, and multifloral). The analytical procedure is based on the matrix solid-phase dispersion of honey in a mixture of Florisil and anhydrous sodium sulfate; the mixture is placed in small plastic columns and extracted with hexane–ethyl acetate (90 + 10, v/v). The pesticide residues are determined by capillary gas chromatography with electron-capture detection. Recoveries with the method at concentrations between 0.15 and 1.5 μg/g ranged from 80 to 113%, and relative standard deviations were <10% for all the pesticides studied. The pesticide detection limits were within the range 0.5–5 mg/kg for organochlorines, around 3 μg/kg for the chlorinated organophosphorus pesticides studied, near 15 μg/kg for fluvalinate, and about 3 μg/kg for the other pyrethroids.
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Battaglia, Christine L. R., Robert M. Gogal, Kurt Zimmerman, and Hara P. Misra. "Malathion, Lindane, and Piperonyl Butoxide, Individually or in Combined Mixtures, Induce Immunotoxicity via Apoptosis in Murine Splenocytes In Vitro." International Journal of Toxicology 29, no. 2 (2010): 209–20. http://dx.doi.org/10.1177/1091581809357954.
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Lindane, malathion, and piperonyl butoxide were cultured singly or as mixtures with murine splenocytes to evaluate changes in cell death and caused cytotoxicity in a concentration- and time-dependent manner. Pesticide mixture studies were then performed based on minimum cytotoxicity concentrations (<LC25). Cytologic analysis and the alamarBlue assay revealed that individual pesticides and mixtures of malathion/lindane and malathion/piperonyl butoxide prompted cytotoxicity, which was supported by DNA ladder analysis. Using 7-aminoactinomycin D, apoptosis was quantified at 6.5%, 12.0%, 13.2%, 19.3%, and 23.4% for malathion, lindane, piperonyl butoxide, malathion-lindane, and malathion-piperonyl butoxide, respectively. Staining with 7-aminoactinomycin D and B- or T-cell–specific fluorescent-labeled monoclonal antibodies showed B cells to be more susceptible to malathion and piperonyl butoxide treatments than T cells. Treatment of murine splenocytes in vitro with minimum cytotoxic concentrations of lindane, malathion, and piperonyl butoxide and their mixtures induced apoptosis, the effect elicited by the mixtures being additive compared with the individual pesticide effect.
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Werner, Inge, Anke Schneeweiss, Helmut Segner, and Marion Junghans. "Environmental Risk of Pesticides for Fish in Small- and Medium-Sized Streams of Switzerland." Toxics 9, no. 4 (2021): 79. http://dx.doi.org/10.3390/toxics9040079.
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This study assessed the acute and chronic risk of pesticides, singly and as mixtures, for fish using comprehensive chemical data of four monitoring studies conducted in small- and medium-sized streams of Switzerland between 2012 and 2018. Pesticides were ranked based on single substance risk quotients and relative contribution to mixture risk. Concentrations of the pyrethroid insecticides, λ-cyhalothrin, cypermethrin and deltamethrin, and the fungicides, carbendazim and fenpropimorph, posed acute or chronic single substance risks. Risk quotients of eighteen additional pesticides were equal or greater than 0.1, and thirteen of those contributed ≥30% to mixture risk. Relatively few substances dominated the mixture risk in most water samples, with chronic and acute maximum cumulative ratios never exceeding 5 and 7, respectively. A literature review of toxicity data showed that concentrations of several pesticides detected in Swiss streams were sufficient to cause direct sublethal effects on fish in laboratory studies. Based on the results of our study, we conclude that pesticides detected in Swiss streams, especially pyrethroid insecticides, fungicides and pesticide mixtures, pose a risk to fish health and can cause direct sublethal effects at environmental concentrations. Sensitive life stages of species with highly specialized life history traits may be particularly vulnerable; however, the lack of toxicity data for non-model species currently prevents a conclusive assessment across species.
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Li, Peng, Qin, and Chao. "A Correction Method of Mixed Pesticide Content Prediction in Apple by Using Raman Spectra." Applied Sciences 9, no. 8 (2019): 1699. http://dx.doi.org/10.3390/app9081699.
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In the study, a new correction method was applied to reduce error during Raman spectral detection on mixed pesticide residue in apples. Combined with self-built pesticide residues detection system by Raman spectroscopy and the application of surface enhancement technology, rapid real-time qualitative and quantitative analysis of deltamethrin and acetamiprid residues in apples could be applied effectively. In quantitative analysis, compared with the intensity value of characteristic peaks of single pesticide with same concentration, the intensity value of characteristic peaks of the two pesticides decreased after mixing the pesticides, which affected the results severely. By comparing the difference in the intensity of characteristic peaks of single and mixed pesticides, a correction method was proposed to eliminate the influence of pesticides mixture. Characteristic peak intensity values of gradient concentration pesticide from 100 mg·kg−1 to 10−3 mg·kg−1 and Lagrangian interpolation were applied in the correction method. And a smooth surface was applied to describe the correction coefficient of characteristic peak intensity. Through detecting the characteristic peak intensity values of the mixed pesticide, correction coefficient would be obtained. Then real values of the peak intensity of pesticides and the content of each component of the mixed pesticide would be acquired by the correction method. Correlation coefficient of model validation exceeded 0.88 generally and Root Mean Square Error also decreased obviously after correction, which proved the reliability of the method.
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Bajaj, Atul, Cijo John, Sukhminder Kaur, and Rohitashva M. Tripathi. "Pesticide Mixture Poisoning: A Case Report." Arab Journal of Forensic Sciences & Forensic Medicine 1, no. 7 (2018): 909–15. http://dx.doi.org/10.26735/16586794.2018.003.
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Qin, Binyi, Zhi Li, Zhihui Luo, Huo Zhang, and Yun Li. "Feasibility of Terahertz Time-Domain Spectroscopy to Detect Carbendazim Mixtures Wrapped in Paper." Journal of Spectroscopy 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/6302868.
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The purpose of this work was to detect carbendazim mixtures wrapped in paper. Unlike previous reports of THz-TDS for detecting pesticide residue, this work focused on detecting pesticide residue in packaged foods. Different weight ratios of carbendazim in polyethylene and in rice powder were detected qualitatively and quantitatively. Results show that pure carbendazim, polyethylene, and rice powder can be easily distinguished from each other. However, when the weight ratio was low, the absorbance of the mixture was similar with that of pure polyethylene and rice powder. With the help of SVM, carbendazim could be qualitatively detected in low weight ratio mixture successfully. Moreover, PLS and SVR were selected to quantitatively detect carbendazim mixtures. SVR has higher R and lower RMSECV, RMSEC, and RMSEP than PLS model. Lasty, the results also indicate that THz-TDS is a potential tool to detect pesticide residue in packaged foods qualitatively and quantitatively.
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Doganlar, Oguzhan, and Banu Doganlar. "Responses of antioxidant enzymes and heat shock proteins in drosophila to treatment with a pesticide mixture." Archives of Biological Sciences 67, no. 3 (2015): 869–76. http://dx.doi.org/10.2298/abs141031046d.
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The effects of a mixture of seven pesticides were examined on the expression of antioxidant enzymes, Mn superoxide dismutase (Mn-SOD), catalase (CAT), glutathione synthetase (GS), and heat shock proteins (HSP) 26, 60, 70 and 83 in adult fruit flies (Drosophila melanogaster Oregon R). The flies were reared under controlled conditions on artificial diets and treated with a mixture of seven pesticides (molinate, thiobencarb, linuron, phorate, primiphos-methyl, fenvalerate and lambda-cyhalothrin) commonly found in water, at concentrations of 0.1, 0.5 and 1 parts per billion (ppb) for 1 and 5 days. Quantitative real-time PCR (qRT-PCR) analysis of Mn-SOD, CAT and GS expression revealed that the analyzed markers responded significantly to pesticide-induced oxidative stress, in particular on the 5th day of treatment. On the 1st day of treatment, the relative expression of HSP26 and HSP60 genes increased only after exposure to the highest concentrations of pesticides, whereas HSP70 and HSP83 expression increased after exposure to 0.5 and 1 ppb. After five days of treatment, the expression of all HSP genes was increased after exposure to all pesticide concentrations. A positive correlation was determined between the relative expression levels of some HSPs (except HSP60), and antioxidant genes. The observed changes in antioxidant enzyme and HSP mRNA levels in D. melanogaster suggest that the permissible limits of pesticide concentrations for clean drinking water outlined in the regulations of several countries are potentially cytotoxic. The presented findings lend support for reevaluation of these limits.
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Atakan, Aydın, Oktay Erdoğan, and Hülya Özgönen Özkaya. "Evaluation of Plant Protection Practices of Garlic Growers in Gaziantep Province." Turkish Journal of Agriculture - Food Science and Technology 8, no. 9 (2020): 1942–47. http://dx.doi.org/10.24925/turjaf.v8i9.1942-1947.3570.
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This study was carried out to determine the plant protection practices of the garlic growers in Gaziantep province. For this purpose, a survey consisting of 20 questions and based on simple random sampling method was conducted with 81 growers in each of the districts of Araban, Oguzeli, Yavuzeli, Central, Nurdagı, Nizip and Karkamış districts in 2018. The data evaluated and expressed as percent ratio. According to the findings from the surveys, garlic growers stated that they preferred pesticide dealers suggestions for the selection and determination of doses of pesticides used in pests and diseases, they also stated that the price and expiration date were not an important factor in the selection of pesticides. In addition, it is found that growers avoid using the same pesticide continuously against same diseases and pests, they do not make any changes in there commended pesticide doses, pesticides leave residues on the products, they paid attention the time between the last application and harvest. It has been reported that the use of protective clothing and mask during the application by the growers is relative, they do not use pesticides as a mixture, they apply different processes to empty pesticide boxes. It was determined that garlic growers prefered chemical control and did not have knowledge about the concept of biopesticides.
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Sgolastra, Fabio, Xavier Arnan, Riccardo Cabbri, et al. "Combined exposure to sublethal concentrations of an insecticide and a fungicide affect feeding, ovary development and longevity in a solitary bee." Proceedings of the Royal Society B: Biological Sciences 285, no. 1885 (2018): 20180887. http://dx.doi.org/10.1098/rspb.2018.0887.
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Pollinators in agroecosystems are often exposed to pesticide mixtures. Even at low concentrations, the effects of these mixtures on bee populations are difficult to predict due to potential synergistic interactions. In this paper, we orally exposed newly emerged females of the solitary bee Osmia bicornis to environmentally realistic levels of clothianidin (neonicotinoid insecticide) and propiconazole (fungicide), singly and in combination. The amount of feeding solution consumed was highest in bees exposed to the neonicotinoid, and lowest in bees exposed to the pesticide mixture. Ovary maturation and longevity of bees of the neonicotinoid and the fungicide treatments did not differ from those of control bees. By contrast, bees exposed to the pesticide mixture showed slow ovary maturation and decreased longevity. We found a synergistic interaction between the neonicotinoid and the fungicide on survival probability. We also found an interaction between treatment and emergence time (an indicator of physiological condition) on longevity. Longevity was negatively correlated to physiological condition only in the fungicide and the mixture treatments. Delayed ovary maturation and premature death imply a shortened nesting period (highly correlated to fecundity in Osmia ). Our findings provide a mechanism to explain the observed dynamics of solitary bee populations exposed to multiple chemical residues in agricultural environments.
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Šlachta, Martin, Tomáš Erban, Alena Votavová, et al. "Domestic Gardens Mitigate Risk of Exposure of Pollinators to Pesticides—An Urban-Rural Case Study Using a Red Mason Bee Species for Biomonitoring." Sustainability 12, no. 22 (2020): 9427. http://dx.doi.org/10.3390/su12229427.
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Domestic gardens supply pollinators with valuable habitats, but the risk of exposure to pesticides has been little investigated. Artificial nesting shelters of a red mason bee species (Osmia bicornis) were placed in two suburban gardens and two commercial fruit orchards to determine the contamination of forage sources by pesticides. Larval pollen provisions were collected from a total of 14 nests. They consisted mainly of pollen from oaks (65–100% weight/sample), Brassicaceae (≤34% w/s) and fruit trees (≤1.6% w/s). Overall, 30 pesticides were detected and each sample contained a mixture of 11–21 pesticide residues. The pesticide residues were significantly lower in garden samples than in orchard samples. The difference was attributed mainly to the abundant fungicides pyrimethanil and boscalid, which were sprayed in fruit orchards and were present on average at 1004 ppb and 648 ppb in orchard samples, respectively. The results suggested that pollinators can benefit from domestic gardens by foraging from floral sources less contaminated by pesticides than in adjacent croplands.
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Kang, Chang An, Le Wang, Xiu Feng Li, et al. "Determination of Multi-Class Pesticide Residues in Lettuce and Cucumber by GC-MSD and the Degradation Study on Vegetables Simultaneously." Advanced Materials Research 998-999 (July 2014): 367–71. http://dx.doi.org/10.4028/www.scientific.net/amr.998-999.367.
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A multi-residue method for determination of pesticide residues in vegetables, using ethyl acetate extraction and determination by means of GC–MSD, is presented. The method includes as many as 27 pesticides such as organophosphorous, organohalogen, organonitrogen and pyrethroids pesticides, which are in common use or forbidden on vegetables in China. After extraction with ethyl acetate, the extract is concentrated and evaporated to dryness and redissolved in ethyl acetate before injection on GC–MSD. The recoveries were obtained in the range 70–100% with 5 exceptions in lettuce and 7 exceptions in cucumber, relative standard deviations between 1.92% and25.39% by means of standard addition to the vegetables blank extracts. The degradation of some pesticides in the 27 was studied by the method developed, the mixture of the pesticides commercial formulations was sparged on the vegetables, then the residues of samples obtained in different time were determined by the developed method. The determination indicated that (all pesticides were used as the instruction): 1.a pesticide, used on different plants, the quantity of the pesticide on the part for people’s dietary differs greatly; 2. some pesticides, degradating slowly and with a large dose, are inappropriate used on vegetables which remain on cropland for a short time. This research does not develop models for pesticides degradation, but provides some data on the safe intervals of the pesticides and a potential method for the study of multi-class pesticides safe intervals on a plant simultaneously.
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Dogheim, Salwa M., Sohair A. Gad Alla, Sami M. A. El-Syes, Monir M. Almaz, and Emel Y. Salama. "Organochlorine and Organophosphonis Pesticide Residues in Food from Egyptian Local Markets." Journal of AOAC INTERNATIONAL 79, no. 4 (1996): 949–52. http://dx.doi.org/10.1093/jaoac/79.4.949.
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Abstract A market basket survey was conducted to monitor organochlorine and organophosphorus pesticide residues in potatoes, citrus fruits, and fish collected from local Egyptian markets. Maximum Residue Limits (MRLs) of the Codex Committee on Pesticide Residues for γ-hexachlorocyclohexane (HCH) in potatoes were exceeded in 8 samples and for DDT in 2 samples. The aging of HCH and DDT indicated a recent use of both pesticides during the potato storage period between cultivation seasons. However, such use is illegal because HCH mixture isomers (gammaxane) and DDT have been officially prohibited from agricultural use in Egypt since 1980. The highest residue levels of fenitrothion (3.8 ppm) in potatoes may be due to its repeated use before and after harvest. No organochlorine pesticide residues were found in citrus fruits. None of the detected organophosphorus pesticides exceeded their MRLs. HCH and DDT residue limits were exceeded in 5 and 7 fish samples, respectively, collected from 12 markets throughout the country. The heptachlor MRL was violated in only one fish sample (3.9 ppm).
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Tosi, S., and J. C. Nieh. "Lethal and sublethal synergistic effects of a new systemic pesticide, flupyradifurone (Sivanto ® ), on honeybees." Proceedings of the Royal Society B: Biological Sciences 286, no. 1900 (2019): 20190433. http://dx.doi.org/10.1098/rspb.2019.0433.
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The honeybee ( Apis mellifera L.) is an important pollinator and a model for pesticide effects on insect pollinators. The effects of agricultural pesticides on honeybee health have therefore raised concern. Bees can be exposed to multiple pesticides that may interact synergistically, amplifying their side effects. Attention has focused on neonicotinoid pesticides, but flupyradifurone (FPF) is a novel butenolide insecticide that is also systemic and a nicotinic acetylcholine receptor (nAChR) agonist. We therefore tested the lethal and sublethal toxic effects of FPF over different seasons and worker types, and the interaction of FPF with a common SBI fungicide, propiconazole. We provide the first demonstration of adverse synergistic effects on bee survival and behaviour (poor coordination, hyperactivity, apathy) even at FPF field-realistic doses (worst-case scenarios). Pesticide effects were significantly influenced by worker type and season. Foragers were consistently more susceptible to the pesticides (4-fold greater effect) than in-hive bees, and both worker types were more strongly affected by FPF in summer as compared with spring. Because risk assessment (RA) requires relatively limited tests that only marginally address bee behaviour and do not consider the influence of bee age and season, our results raise concerns about the safety of approved pesticides, including FPF. We suggest that pesticide RA also test for common chemical mixture synergies on behaviour and survival.
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Tatarkovičová, Věra, and Radek Machač. "Investigation of Conditions for the Determination of Pesticides in Water Using Solid Phase Extraction." Collection of Czechoslovak Chemical Communications 57, no. 11 (1992): 2295–301. http://dx.doi.org/10.1135/cccc19922295.
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The conditions for the preconcentration of carbamate, phenylurea and triazine pesticides from water samples were investigated, and optimized methods were developed for the determination of the pesticide groups. Bakerbond spe C18 cartridges were used for the preconcentration. The preconcentrated carbamate and phenylurea pesticides were eluted from the cartridges with acetone, whereas the triazine herbicides were eluted with chloroform. After additional concentration by evaporation, the eluates were analyzed by RC-HPLC on an AQUAPORE RP-300 column using a methanol-water mixture as the mobile phase.
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Juntarawijit, Yuwayong, and Chudchawal Juntarawijit. "Pesticide exposure and rhinitis: A cross-sectional study among farmers in Pitsanulok, Thailand." F1000Research 10 (June 15, 2021): 474. http://dx.doi.org/10.12688/f1000research.53257.1.
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Background: Pesticide exposure has been suspected to cause rhinitis, a common disease that affects the health and wellbeing of millions of people around the world. The objective of this cross-sectional study was to examine the association between pesticide use and rhinitis prevalence among farmers in Phitsanulok province, Thailand. Methods: Data on pesticide use and rhinitis use were collected by an in-person interview questionnaire. Data from 9,649 participants were included in the analysis. The association between pesticide exposure and rhinitis was determined by multiple variable logistic regression, adjusted for potential confounding factors. Results: It was found that 6.3% of study participants had rhinitis, which was defined as having one or more of the following symptoms: sneezing, runny nose, and nasal congestion, within the past year. The study found each type of pesticide (insecticides, herbicides, fungicides, rodenticides, molluscicides) to be significantly associated with rhinitis, with the odds ratio ranging from 1.67 (95% CI 1.41-1.99) for the fungicide group to 7.19 (95% CI 4.67-11.06) for insecticide. For individual pesticides, the study found 33 out of 35 to be significantly associated with rhinitis with the OR values ranging from 1.30 (95% CI 1.00-1.69) for alachlor to 5.67 (95% CI 3.16-10.16) for Bordeaux mixture. Conclusions: This study found a prevalence of rhinitis among farmers in Phitsanulok, Thailand to be associated with the long-term exposure of many individual pesticides. This finding was consistent with other literature. Effects of pesticide on rhinitis should receive more attention from the public health and other organizations responsible for the farmers’ health.
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Vela, Nuria, Gabriel Prez, Gins Navarro, and Simn Navarro. "Gas Chromatographic Determination of Pesticide Residues in Malt, Spent Grains, Wort, and Beer with Electron Capture Detection and Mass Spectrometry." Journal of AOAC INTERNATIONAL 90, no. 2 (2007): 544–49. http://dx.doi.org/10.1093/jaoac/90.2.544.
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Abstract A method for the simultaneous determination of 9 pesticides (dinitroanilines, organophosphorus, triazoles, and pyrimidines) in several products (malt, spent grains, wort, and beer) of the beer industry is reported. Solid samples (malt and spent grains) are extracted by homogenization with a waterhexane mixture, and the pesticides are partitioned with dichloromethane. Liquid samples (wort and beer) are extracted by sonication with a hexanedichloromethane mixture. Determination of pesticide residues was made by capillary gas chromatography with an electron capture detector (ECD). Confirmation of the compounds was performed by gas chromatography/ion trap mass spectrometry in the selected ion monitoring mode. Detection limits for GCECD varied from 0.2 to 5.5 pg for trifluralin and malathion, respectively. Recoveries of the pesticides from spiked samples ranged from 81.2 to 113.7% with a relative standard deviation between 3.47.5%. The method presents good linearity over the studied range (0.0052 g/mL). The proposed method is rapid and reliable and can be useful for routine monitoring during brewing.
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Lash, Heather E., Daniel F. Warnock, and Raymond A. Cloyd. "Effect of Pesticide Mixtures on the Survival of the Predatory Mite Neoseiulus cucumeris (Acarina: Phytoseiidae)." Journal of Entomological Science 42, no. 3 (2007): 311–19. http://dx.doi.org/10.18474/0749-8004-42.3.311.
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This study was conducted to determine the effect of 2 insecticides/miticides, spinosad and abamectin, and 2 fungicides, thiophanate-methyl and fenhexamid, when applied alone or mixed in all combinations, on the survival of Neoseiulus cucumeris (Oudemans). Neoseiulus cucumeris is a predatory mite utilized in greenhouses for managing western flower thrips, Frankliniella occidentalis Pergande. The deutonymph and adult stages of N. cucumeris were directly exposed to the pesticide treatments or a water control. Overall, the deutonymphs were more susceptible to the pesticide treatments, based on percent survival 24 h after treatment, than the adults. Five of the 7 pesticide mixtures containing spinosad and 4 of the 7 mixtures containing abamectin resulted in deutonymph mortality values between 50 and 69%. Thiophanate-methyl alone and in combination with either spinosad or abamectin resulted in &gt;35% mortality of the deutonymphs. Based on the results of this study, spinosad and abamectin, when used alone or in mixture with thiophanate-methyl will reduce N. cucumeris populations by killing the deutonymphs. This information is important to greenhouse managers who want to use pesticide mixtures to manage arthropod pests and fungal pathogens without disrupting biological control programs for western flower thrips.
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Gamón, Miguel, Concha Lleó, Amparo Ten, and Francisco Mocholí. "Multiresidue Determination of Pesticides in Fruit and Vegetables by Gas Chromatography/Tandem Mass Spectrometry." Journal of AOAC INTERNATIONAL 84, no. 4 (2001): 1209–16. http://dx.doi.org/10.1093/jaoac/84.4.1209.
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Abstract Pesticide residues in fruit and vegetables were determined by gas chromatography/tandem mass spectrometry (GC/MS/MS). Electron impact (EI)/MS/MS and chemical ionization (CI)/MS/MS were developed for 80 compounds, including organochlorine, organophosphorus, organonitrogen, and pyrethroids, providing unambiguous spectral confirmation for these complex matrixes. Residues were extracted from samples with acetone followed by a mixture of dichloromethane–petroleum ether. Two injections per sample were required for analysis of the entire pesticide list by EI/MS/MS and CI/MS/MS.Initial steps involving cleanup and concentration of extracts were eliminated. The excellent selectivity and good linearity allowed quantification and identification of low levels of pesticides in the most difficult matrixes. The method has been used for routine analysis of many vegetables.
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Rama Krishna, K., and Ligy Philip. "Bioremediation of Single and Mixture of Pesticide-Contaminated Soils by Mixed Pesticide-Enriched Cultures." Applied Biochemistry and Biotechnology 164, no. 8 (2011): 1257–77. http://dx.doi.org/10.1007/s12010-011-9211-5.
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Hoai Bui, Thu, Maithaa Karkmaz, Eric Puzenat, Chantal Guillard, and Jean-Marie Herrmann. "EFICIENCIA SOLAR DE UN NUEVO FOTO-CATALIZADOR TiO2 DEPOSITADO SOBRE UN PAPEL FOTO-INERTE PARA LA DEGRADACIÓN TOTAL DE VARIOS PLAGUICIDAS Y COLORANTES." Revista Cientifica TECNIA 20, no. 2 (2017): 5. http://dx.doi.org/10.21754/tecnia.v20i2.100.
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ABSTRACTA specially designed titania photocatalyst was prepared by coating Ahlstrom non-woven paper, used as a flexible photocatalytic support, with Millennium PC500 anatase. At the same time, a new solar photoreactor (STEP) was designed based on the multi-step cascade falling-film principle to ensure good exposure to sunlight and good oxygenation of the effluent to be treated. Several types of reactants were treated: 4-chlorophenol as a model organic pollutant; formetanate, a widely used pesticide in horticulture; a mixture of pesticides used in vineyards; and Indigo Carmine and Congo Red, which are complex multifunctional dye molecules. Each reaction was performed simultaneously in a solar CPC slurry photoreactor and in the STEP photoreactor under identical solar exposure to better evaluate and validate the results obtained. The STEP solar reactor was found to be as efficient as the CPC one for 4-chlorophenol and formetanate total degradation. Keywords.-Pesticide, Degradation, Photocatalyst. RESUMENUn diseño especial de un fotocalizador de TiO2 fue depositado sobre un papel foto inerte. Al mismo tiempo un nuevo fotoreactor solar (STEP) fue diseñado en base a una cascada multipaso, generando buenos resultados a la exposición de la luz solar con buena oxigenación del efluente que se trato. Se utilizaron como reactivos a tratar: 4- clorofenol como un contaminante orgánico, formetano ampliamente usado como pesticida en horticultura, y una mezcla de pesticidas usadas en viñedos; así mismo el índigo carmín y congo red como complejos multifuncional de colorantes. Cada reacción fue realizada en un reactor STEP con agitación y exposición solar CPC simultáneamente para tener una mejor evaluación de los resultados obtenidos. El reactor solar STEP fue muy eficiente como el CPC, para el 4-clorofenol y formetano se obtuvo una degradación total. Palabras clave.- Plaguicidas, Degradación, Fotocatalizador.
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GarcÍa-Repetto, Rosario, M. Paz Giménez, and Manuel Repetto. "New Method for Determination of Ten Pesticides in Human Blood." Journal of AOAC INTERNATIONAL 84, no. 2 (2001): 342–49. http://dx.doi.org/10.1093/jaoac/84.2.342.
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Abstract An analytical method was developed for precise identification and quantitation of 10 pesticides in human blood. The pesticides studied, which have appeared frequently in actual cases, were endosulfan, lindane, parathion, ethyl-azinphos, diazinon, malathion, alachlor, tetradifon, fenthion and dicofol (o-p′ and p-p′ isomers). The current method replaces an earlier method which involved liquid–liquid extraction with a mixture of n-hexane–benzene (1 + 1). The extraction is performed by solid-phase extraction, with C18 cartridges and 2 internal standards, perthane and triphenylphosphate. Eluates were analyzed by gas chromatography (GC) with nitrogen–phosphorus and electrochemical detectors. Results were confirmed by GC–mass spectrometry in the electron impact mode. Blood blank samples spiked with 2 standard mixtures and an internal standard were used for quantitation. Mean recoveries ranged from 71.83 to 97.10%. Detection and quantitation limits are reported for each pesticide. Examples are provided to show the application of the present method to actual samples.
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Kida, Małgorzata, Sabina Ziembowicz, and Piotr Koszelnik. "Application of an ultrasonic field for the removal of selected pesticides." E3S Web of Conferences 49 (2018): 00054. http://dx.doi.org/10.1051/e3sconf/20184900054.
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The work detailed in this study investigated the efficiency of oxidizing technologie for the removal of organochlorine pesticides (OCPs) from aqueous solutions. OCPs constitute an important group of environmental pollutants also known to be endocrine-disrupting compounds (EDCs). The particular focus has been on a process achieving oxidation through the use of ultrasonic waves, with the impacts of pH, time on TOC and pesticide removal all being tested. Results confirm effective TOC decomposition using ultrasonic waves, albeit with reaction time showing a significant impact on results. Furthermore, results for TOC were not found to reflect those relating to the removal of individual substances from the tested mixture. Chromatographic analysis of reaction mixtures was therefore carried out after the selected processes had been applied, and an attempt to identify and characterize degradation products of the pesticides tested was also made. During the irradiation, the H2O2 concentration arising was also monitored. The results indicate that H2O2 is produced by cavitation during ultrasonic irradiation.
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Landgren, Ola, Robert A. Kyle, Jane A. Hoppin, et al. "Pesticide exposure and risk of monoclonal gammopathy of undetermined significance in the Agricultural Health Study." Blood 113, no. 25 (2009): 6386–91. http://dx.doi.org/10.1182/blood-2009-02-203471.
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Abstract Pesticides are associated with excess risk of multiple myeloma, albeit inconclusively. We included 678 men (30-94 years) from a well-characterized prospective cohort of restricted-use pesticide applicators to assess the risk of monoclonal gammopathy of undetermined significance (MGUS). Serum samples from all subjects were analyzed by electrophoresis performed on agarose gel; samples with a discrete or localized band were subjected to immunofixation. Age-adjusted prevalence estimates of MGUS were compared with MGUS prevalence in 9469 men from Minnesota. Associations between pesticide exposures and MGUS prevalence were assessed by logistic regression models adjusted for age and education level. Among study participants older than 50 years (n = 555), 38 were found to have MGUS, yielding a prevalence of 6.8% (95% CI, 5.0%-9.3%). Compared with men from Minnesota, the age-adjusted prevalence of MGUS was 1.9-fold (95% CI, 1.3- to 2.7-fold) higher among male pesticide applicators. Among applicators, a 5.6-fold (95% CI, 1.9- to 16.6-fold), 3.9-fold (95% CI, 1.5- to 10.0-fold), and 2.4-fold (95% CI, 1.1- to 5.3-fold) increased risk of MGUS prevalence was observed among users of the chlorinated insecticide dieldrin, the fumigant mixture carbon-tetrachloride/carbon disulfide, and the fungicide chlorothalonil, respectively. In summary, the prevalence of MGUS among pesticide applicators was twice that in a population-based sample of men from Minnesota, adding support to the hypothesis that specific pesticides are causatively linked to myelomagenesis.
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Mahugija, John A. M., Farhat A. Khamis, and Esther H. J. Lugwisha. "Determination of Levels of Organochlorine, Organophosphorus, and Pyrethroid Pesticide Residues in Vegetables from Markets in Dar es Salaam by GC-MS." International Journal of Analytical Chemistry 2017 (2017): 1–9. http://dx.doi.org/10.1155/2017/4676724.
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This study investigated the levels of pesticides and metabolites in vegetables from major markets in Dar es Salaam city, Tanzania. Samples of fresh cabbage, spinach, and onions from the markets were analysed for pesticide residues. Extraction was performed using acetone followed by dichloromethane : cyclohexane mixture and the extracts were cleaned up using Florisil. The compounds were determined by gas chromatography-mass spectrometry (GC-MS). Pesticides and metabolites were detected in 72.2% of the samples. The detected pesticide residues and their highest mean concentrations were p,p′-DDT 4.00 × 10−3 mg/kg, p,p′-DDD 6.40 × 10−1 mg/kg, o,p′-DDD 1.00 × 10−2 mg/kg, α-endosulfan 6.00 × 10−1 mg/kg, β-endosulfan 2.10 × 10−1 mg/kg, chlorpyrifos 3.00 mg/kg, and cypermethrin 4.00 × 10−2 mg/kg. The most frequently detected compounds were p,p′-DDD and chlorpyrifos. The order of contamination was spinach > cabbage > onions. Generally, there were no significant variations in concentrations of pesticide residues among samples and sampling sites, which indicated similarities in contamination patterns. The concentrations of contaminants were above the maximum residue limits (MRLs) in 33.3–50% of the samples. The findings indicated risks and concerns for public health.
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Zhang, Jin, Shu Shen Liu, Hui Ping Deng, Shu Guang Zhu, and Qi Ling. "Synergistic Effect of Aldicarb on the Toxicity of Ionic Liquid Mixtures to Vibrio qinghaiensis sp.-Q67." Advanced Materials Research 864-867 (December 2013): 413–17. http://dx.doi.org/10.4028/www.scientific.net/amr.864-867.413.
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Various chemicals emerge in environment and exist as mixtures with different forms and concentrations. Toxicity interaction by mixtures may pose potential hazards and risks to the environmental safety and human health. Recent data show that toxicity interaction within ionic liquid (IL) mixtures related with some certain component. However, how to determine the specific component in a mixture is not an easy work and needs more researches about it. Therefore, we investigated the toxicity of twelve groups of mixtures by using three ILs and one pesticide aldicarb as mixture components. Four binary and eight pseduo-binary mixtures were designed by equivalent effect concentration ratio ray method using EC5and EC50of individual component. The toxicities of single chemicals and these mixtures to a freshwater photobacteriumVibrio qinghaiensissp.-Q67 were determined by using the microplate toxicity analysis method. Toxicity interaction within mixtures were determined based on an additive referrence model, concentration addition (CA). The results showed that three binary IL mixture exhibited additive action and one antagonism. Interstingly, most pusedo-mixtures of aldicab and binary IL mixture exhibited cleary synergism especialy when they were mixed in the ratio of components EC5.
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Gangola, Saurabh, Samiksha Joshi, Saurabh Kumar, Barkha Sharma, and Anita Sharma. "Differential proteomic analysis under pesticides stress and normal conditions in Bacillus cereus 2D." PLOS ONE 16, no. 8 (2021): e0253106. http://dx.doi.org/10.1371/journal.pone.0253106.
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A potential pesticide degrading bacterial isolate (2D), showing maximum tolerance (450 mg∙L-1) for cypermethrin, fipronil, imidacloprid and sulfosulfuron was recovered from a pesticide contaminated agricultural field. The isolate degraded cypermethrin, imidacloprid, fipronil and sulfosulfuron in minimal salt medium with 94, 91, 89 and 86% respectively as revealed by high performance liquid chromatography (HPLC) and gas chromatography (GC) analysis after 15 days of incubation. Presence of cyclobutane, pyrrolidine, chloroacetic acid, formic acid and decyl ester as major intermediate metabolites of cypermethrin biodegradation was observed in gas chromatography mass spectrometry (GC-MS) analysis. Results based on 16S rDNA sequencing, and phylogenetic analysis showed maximum similarity of 2D with Bacillus cereus (Accession ID: MH341691). Stress responsive and catabolic/pesticide degrading proteins were over expressed in the presence of cypermethrin in bacteria. Enzymatic kinetics of laccase was deduced in the test isolate under normal and pesticide stress conditions which suggested that the production of enzyme was induced significantly in pesticide stress (163 μg.μL-1) as compare to normal conditions(29 μg.μL-1) while the Km value was decreased in pesticides stress condition (Km = 10.57 mM) and increases in normal condition (Km = 14.33 mM).Amplification of laccase gene showed a major band of 1200bp. The present study highlights on the potential of 2D bacterial strain i.e., high tolerance level of pesticide, effective biodegradation rate, and presence of laccase gene in bacterial strain 2D, could become a potential biological agent for large-scale treatment of mixture of pesticide (cypermethrin, fipronil, imidacloprid and sulfosulfuron) in natural environment (soil and water).
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Delnat, Vienna, Lizanne Janssens, and Robby Stoks. "Effects of predator cues and pesticide resistance on the toxicity of a (bio)pesticide mixture." Pest Management Science 76, no. 4 (2019): 1448–55. http://dx.doi.org/10.1002/ps.5658.
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Huang, Zhengyu, and Kimberly Ann Falco. "Synergy Assessment for Plant Growth by Independent Joint Action Theory." HortScience 56, no. 5 (2021): 623–26. http://dx.doi.org/10.21273/hortsci15731-21.
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Calculating the predicted biological efficacy of a mixture and determining the significance of the difference between the predicted efficacy and the measured efficacy of that mixture are fundamental when assessing the synergy of mixtures. The Independent Joint Action theory and Bliss’s formula are well-known and widely accepted for predicting pesticide mixture effects that are expressed in terms of percent mortality. Bliss’s formula, however, is not applicable to growth-affecting components, such as plant growth regulators. Therefore, there is an unmet need of critical importance: an appropriate method for assessing synergy of growth-affecting mixtures needs to be identified within the scientific community. The formula, G(1 + 2..n) = G1G2…Gn/(GCTL)n−1, which was derived from the Independent Joint Action theory, is presented for calculating the predicted efficacy for mixtures of growth-affecting components that either promote or inhibit growth. Its application is demonstrated by analyzing data from a greenhouse assay in which a mixture of S-abscisic acid and gibberellic acid was used to promote the growth of corn seedlings.
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Zhang, Jing, Wenqiang Wang, Zhoutao Pei, et al. "Mutagenicity Assessment to Pesticide Adjuvants of Toluene, Chloroform, and Trichloroethylene by Ames Test." International Journal of Environmental Research and Public Health 18, no. 15 (2021): 8095. http://dx.doi.org/10.3390/ijerph18158095.
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Pesticide adjuvants (PAs) denote the general term for auxiliaries in pesticide preparations except for the active components. Toluene, chloroform, and trichloroethylene are the three most commonly used PAs as organic solvents. The residues of the three chemicals in the process of production and application of pesticides may endanger the ecosystem. In the present study, the mutagenicity of toluene, chloroform, and trichloroethylene as well the mixture of the three chemicals was tested by the Salmonella typhimurium reverse mutation test (Ames test) with TA97, TA98, TA100, and TA102 strains in the system with and without rat liver microsomal preparations (S9). The four tester strains have been used for more than 40 years to detect mutagenic compounds in chemicals, cosmetics, and environmental samples. The mutagenicity was detected on tester strains in the separated experiment from the three chemicals. The addition of S9 decreased the mutation ratios of toluene to four strains, except for the TA100 strain, but increased the mutation ratios of chloroform to four strains except for the TA98 strain. Trichloroethylene caused positive mutagenicity to become negative on the TA102 strain. In the mixed experiment, positive effects were detected only on the TA102 strain in the absence of S9. The addition of S9 increased the mutagenicity except for the TA102 strain. The mixture of toluene, chloroform, and trichloroethylene showed antagonism in mutagenicity to tester strains, except for the TA102 strain without S9. However, the mixture showed a synergistic effect to tester strains after adding S9 except for the TA98 strain.
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Levio-Raiman, Marcela, Gabriela Briceño, Bárbara Leiva, et al. "Treatment of Pesticide-Contaminated Water Using a Selected Fungal Consortium: Study in a Batch and Packed-Bed Bioreactor." Agronomy 11, no. 4 (2021): 743. http://dx.doi.org/10.3390/agronomy11040743.
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This study provides the basis for implementing a continuous treatment system for wastewater containing a pesticide mixture formed by atrazine, iprodione, and chlorpyrifos. Two fungal strains (Verticilium sp. H5 and Metacordyceps sp. H12) isolated from a biomixture of a biopurification system were able to remove different pesticide concentrations (10 to 50 mg L−1) efficiently from the liquid medium; however, the half-life of the pesticides was reduced and characterized by a T1/2 of 5.4 to 9.2 d for atrazine, 3.7 to 5.8 d for iprodione, and 2.6 to 2.9 d for chlorpyrifos using the fungal consortium. The immobilization of the fungal consortium in alginate bead was effective, with the highest pesticide removal observed using an inoculum concentration of 30% wv−1. The packed-bed reactor with the immobilized fungal consortium, which was operated in the continuous mode at different flow rates (30, 60, and 90 mL h−1), required approximately 10 d to achieve removal efficiency (atrazine: 59%; iprodione: 96%; chlorpyrifos: ~85%). The bioreactor was sensitive to flow rate fluctuations but was able to recover performance quickly. The pesticide metabolites hydroxyatrazine, 3,5-dichloroaniline, and 3,5,6-trichloro-2-pyridinol were produced, and a slight accumulation of 3,5,6-trichloro-2-pyridinol was observed. Nevertheless, reactor removal efficiency was maintained until the study ended (60 d).
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Zhang, Zhihong, Heping Zhu, and Chengsong Hu. "Hardware and Software Design for Premixing In-Line Injection System Attached to Variable-Rate Orchard Sprayer." Transactions of the ASABE 63, no. 4 (2020): 823–21. http://dx.doi.org/10.13031/trans.13730.
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.HighlightsA premixing in-line injection system was designed as a retrofit attachment to a laser-guided variable-rate orchard sprayer.A graphical user interface with touchscreen functions was incorporated into the system to facilitate field applications.Preliminary tests demonstrated that simulated pesticide and water could be accurately dispensed and discharged separately into the injection line and mixed well in the buffer tank.This premixing in-line injection system has great potential to further reduce pesticide waste and improve environmental stewardship for conventional and precision variable-rate sprayers.Abstract To eliminate the problems associated with leftover tank mixture in pesticide applications, a premixing in-line injection system was designed as an attachment to a laser-guided variable-rate orchard sprayer. The primary components of the system consisted of a chemical metering pump, a water pump, a two-stage static mixer, a premixing tank, a buffer tank, an electric shut-off valve, a chemical container, electronic control boards, a graphical user interface, and an embedded computer with a touch screen. Liquid level sensors were mounted in all tanks and the chemical container to control the fluid discharge and prevent overflows. The graphical user interface on the touch screen was designed for operators to communicate with the system and monitor the system status. During spray applications, the system performed with automatic loops in dispensing, mixing, and transferring the desired amounts of water and chemical concentrates to maintain the spray mixture at a constant concentration for use with variable-rate nozzles. The system was rinsed automatically when the spray application task was completed. Test results showed that simulated pesticide and water could be accurately delivered into the injection line and could be mixed well in the buffer tank before the spray mixture was discharged to the nozzles. The premixing in-line injection system is a potential technique to further reduce pesticide waste and improve environmental stewardship for both conventional and precision variable-rate orchard sprayers. Keywords: Automation, Environmental protection, Intelligent sprayer, Pesticide spray application, Precision agriculture, Tank mixture leftover.
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Chaklader, Malay, Prosun Das, Jacintha Archana Pereira, Samaresh Chaudhuri, and Sujata Law. "Altered canonical hedgehog-gli signalling axis in pesticide-induced bone marrow aplasia mouse model." Archives of Industrial Hygiene and Toxicology 63, no. 3 (2012): 271–82. http://dx.doi.org/10.2478/10004-1254-63-2012-2255.
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The mechanistic interplay between pesticide exposure and development of marrow aplasia is not yet well established but there are indices that chronic pesticide exposure in some instances causes marrow aplasia like haematopoietic degenerative condition in human beings. Canonical Hedgehog (Hh) signalling has multiple roles in a wide range of developmental processes, including haematopoiesis. The present study was designed to explore the status of four important components of the canonical Hedgehog signalling cascade, the Sonic Hedgehog (Shh), Ptch1, Smo, and Gli1, in a mouse model of chronic pesticide-induced bone marrow aplasia. We used 5 % aqueous mixture of pesticides (chlorpyriphos, prophenophos, cypermethrin, alpha-methrin, and hexaconazole) for inhalation and dermal exposure of 6 hours per day and 5 days a week up to 90 days. Murine bone marrow aplasia related to chronic pesticide treatment was confi rmed primarily by haemogram, bone marrow cellularity, short term bone marrow explant culture for cellular kinetics, bone marrow smear, and fl ow cytometric Lin-Sca-1+C-kit+ extracellular receptor expression pattern. Later, components of hedgehog signalling were analysed in the bone marrow of both control and pesticide-treated aplastic groups of animals. The results depicted pancytopenic feature of peripheral blood, developmental anomaly of neutrophils, depression of primitive stem and progenitor population along with Shh, Ptch1, Smo and Gli1 expression in aplasia group. This investigation suggests that pesticide-induced downregulation of two critically important proteins - Ptch1 and Gli1 - inside the haematopoietic stem and progenitor cell population impairs haematopoietic homeostasis and regeneration mechanism in vivo concurrent with bone marrow aplasia.
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Mansour, Sameeh A., Alia A. Abdel-Hamid, Azza W. Ibrahim, Neveen H. Mahmoud, and Walaa A. Moselhy. "Toxicity of Some Pesticides, Heavy Metals and Their Mixtures to Vibrio fischeri Bacteria and Daphnia magna: Comparative Study." Journal of Biology and Life Science 6, no. 2 (2015): 221. http://dx.doi.org/10.5296/jbls.v6i2.8174.
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The present study was conducted to evaluate the sensitivity of the Vibrio fischeri bioluminescence inhibition test (Microtox® assay), and the standard acute Daphnia magna test; using 3 heavy metals, 3 organic pesticides, and their mixtures. In Daphnia tests, either at 24h or 30 min exposure times, the pattern of toxicity order for heavy metals was Cu ˃ Cd ˃ Pb. Chlorpyrifos-methyl was the highest toxic at 24h, while Triazophos was the highest toxic at 30 min exposure times. In the Microtox® test at 5 min exposure time, the estimated EC50 values were 4.20, 4.53 and 6.60 mg/L for Cu, Cd and Pb, respectively. At the same exposure time, the EC50 values of Triazophos, Chlorpyrifos-Me and Profenofos accounted to 1.76, 3.36 and 4.12 mg/L, respectively. Similar order of toxicity was obtained when tests were conducted at 15 min exposure time. The paired mixtures of pesticides, as well as the mixtures of Cu + Cd and Pb + Cd, showed potentiation effects, while the mixture of Cu + Pb showed additive effect against D. magna. The tertiary mixtures of the pesticides or the heavy metals reacted antagonistically. In the Microtox® assay, the heavy metal mixtures reacted antagonistically, while pesticide mixtures showed synergism. It was concluded that both Daphnia and Microtox® tests showed similar pattern of sensitivity to the single toxicants, but dissimilar pattern to the heavy metal mixtures. On the other side, using shorter exposure time (ca. 30 min) with Daphnia bioassay may enable us to held reliable comparisons with Microtox® results.
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Fustinoni, S., L. Campo, J. Liesivuori, et al. "Biological monitoring and questionnaire for assessing exposure to ethylenebisdithiocarbamates in a multicenter European field study." Human & Experimental Toxicology 27, no. 9 (2008): 681–91. http://dx.doi.org/10.1177/0960327108100003.
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This study deals with pesticide exposure profile in some European countries with a specific focus on ethylenebisdithiocarbamates (EBDC). In all, 55 Bulgarian greenhouse workers, 51 Finnish potato farmers, 48 Italian vineyard workers, 42 Dutch floriculture farmers, and 52 Bulgarian zineb producers entered the study. Each group was matched with a group of not occupationally exposed subjects. Exposure data were gained through self-administered questionnaires and measuring ethylenethiourea (ETU) in two spot urine samples collected, respectively, before the beginning of seasonal exposure (T0), and after 30 days, at the end of the exposure period (T30). Controls underwent a similar protocol. Study agriculture workers were involved in mixing and loading pesticides, application of pesticide mixture with mechanical or manual equipments, re-entry activities, and cleaning equipments. Chemical workers were involved in synthesis, quality controls, and packing activities. The number of pesticides to whom these subjects were exposed varied from one (zineb production) to eight (potato farmers). The use of personal protective devices was variegate and regarded both aerial and dermal penetration routes. EBDC exposure, assessed by T30 urinary ETU, was found to follow the order: greenhouse workers, zineb producers, vineyard workers, potato farmers, floriculture farmers with median levels of 49.6, 23.0, 11.8, 7.5, and 0.9 μg/g creatinine; the last group having ETU at the same level of controls (~0.5 μg/g creatinine). Among agriculture workers, pesticide application, especially using manual equipment, seems to be the major determinant in explaining internal dose. Although the analysis of self-administered questionnaires evidenced difficulties especially related to lack and/or poor quality of reported data, biological monitoring confirms to be a powerful tool in assessing pesticide exposure.
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Monsalvo, V. M., N. Garcia-Mancha, D. Puyol, A. F. Mohedano, and J. J. Rodriguez. "Anaerobic biodegradability of mixtures of pesticides in an expanded granular sludge bed reactor." Water Science and Technology 69, no. 3 (2013): 532–38. http://dx.doi.org/10.2166/wst.2013.739.
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The biodegradability and toxicity of three commercial pesticides containing 2-methyl-4-chlorophenoxyacetic acid (MCPA), imidacloprid and dimethoate were evaluated individually, and a complex mixture of these pesticides was treated in an expanded granular sludge bed (EGSB) reactor. MCPA was partially biodegraded, while imidacloprid and dimethoate remained almost unaltered during the individual biodegradability tests. Cyclohexanone was identified as the major solvent in the dimethoate-bearing insecticide, which was completely removed regardless of the presence of other pesticides. The analysis of the inhibition over the acetoclastic methanogenesis showed IC50 (half maximal inhibitory concentration) values of 474 and 367 mg/L for imidacloprid and dimethoate, respectively. The effect on the methanogenesis was negligible in the case of MCPA and cyclohexanone. Pesticides caused a dramatic decrease of the EGSB reactor performance. After 30 d acclimation, the EGSB reactor achieved a stable chemical oxygen demand (COD) removal efficiency and methane production of around 85% and 0.9 g CH4-COD/g COD, respectively, for MCPA, imidacloprid, dimethoate and cyclohexanone feed concentrations of 57, 20, 25 and 27 mg/L, respectively. The presence of complex pesticide mixtures led to synergistic/antagonistic responses, reducing the MCPA biodegradation and improving the removal of the insecticides' active ingredients, which were completely removed in the EGSB reactor.
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Peng, C., H. Kuang, X. Li, and C. Xu. "Evaluation and interlaboratory validation of a GC-MS method for analysis of pesticide residues in teas." Chemical Papers 61, no. 1 (2007): 1–5. http://dx.doi.org/10.2478/s11696-006-0086-9.
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AbstractA method was described for simultaneous determination of nine organic heterocyclic pesticide residues by gas chromatography-mass spectrometry-selected ion monitoring. Atrazine, vinclozolin, procymidone, triflumizole, imazalil, buprofezin, propiconazole, fenarimol, and pyridaben were clearly separated from each other, extracted with acetone—hexane mixture, purified with graphitized carbon black cartridge and neutral Al2O3 cartridge, eluted with acetone—hexane mixture, simultaneously determined by GC-MS, and then quantified with an external standard method. Recoveries of pesticides ranged from 73 % to 116 % at the spiked level of 0.01–30 mg kg−1, while the relative standard deviation was between 3 % and 27 %. In addition, the limits of determination (0.01 mg kg−1 to 5.0 mg kg−1) and linearity (0.02–40 μg mL−1) revealed that simultaneous determination of multi-residues in Chinese teas (like Oolong tea, green tea, red tea, etc.) was possible. Furthermore, an interlaboratory study among 5 labs was conducted to further validate the method, and the results were satisfactory.
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Tierney, Keith B., Jessica L. Sampson, Peter S. Ross, Mark A. Sekela, and Christopher J. Kennedy. "Salmon Olfaction is Impaired by an Environmentally Realistic Pesticide Mixture." Environmental Science & Technology 42, no. 13 (2008): 4996–5001. http://dx.doi.org/10.1021/es800240u.
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Moser, Virginia C., Jane Ellen Simmons, and Chris Gennings. "Neurotoxicological Interactions of a Five-Pesticide Mixture in Preweanling Rats." Toxicological Sciences 92, no. 1 (2006): 235–45. http://dx.doi.org/10.1093/toxsci/kfj189.
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Juan-Borrás, Marisol, Eva Domenech, and Isabel Escriche. "Mixture-risk-assessment of pesticide residues in retail polyfloral honey." Food Control 67 (September 2016): 127–34. http://dx.doi.org/10.1016/j.foodcont.2016.02.051.
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Schmidt, Felix F., Dajana Lichtenstein, Hannes Planatscher, et al. "Pesticide mixture effects on liver protein abundance in HepaRG cells." Toxicology 458 (June 2021): 152839. http://dx.doi.org/10.1016/j.tox.2021.152839.
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WElkner, Krystyna, and Jerzy Szwejda. "Effect of pesticide mixture on value of fresh, fermented and pickles cucumbers." Acta Agrobotanica 54, no. 1 (2013): 191–207. http://dx.doi.org/10.5586/aa.2001.016.
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In years 1998-1999 effects of fungicides (azoxystrobin and copper hydroxide), insecticides (etafenproks and pirimicarb) and their mixtures on chemical composition and organoleptic quality of fresh, fermented and pickled cucumbers were studied_ To analyses fruits were taken after 7 and 12 days from treatment. Fresh cucumber fruits after 7 days from treatment with investigated pesticides contained less carbohydrates and ascorbic acids and higher level of nitrates in compare to untreated fruits (control). After 12 days from treatment the differences between treated and untreated fruits dissapeared. Cucumber fruits harvested 7 and 12 days after treatment were processed separately. Quality evaluation of fermented and pickled cucumbers was carried out 4 months after processing. In most of cucumber fruits (7 days after treatment with pesticides) treated with pesticides empty cavities were found, and not in control (untreated). Number of cavities was highest in fresh and pickled cucumbers treated with mixture of fungicide and insecticide. In case of fermented cucumbers highest number of empty cavities occurred in fruits treated with fungicide azoxystrobin. Cucumber fruits taken to fermentation process 7 days after day of treatment had darker green colour, lowered haIdness, and larger empty cavities than untreated fruits. In fruits harvested 12 days after pesticides application such differencess were not observed.