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1

Varjani, Sunita J. "Microbial degradation of petroleum hydrocarbons." Bioresource Technology 223 (January 2017): 277–86. http://dx.doi.org/10.1016/j.biortech.2016.10.037.

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2

Li, Xingchun, Wei He, Meijin Du, Jin Zheng, Xianyuan Du, and Yu Li. "Design of a Microbial Remediation Inoculation Program for Petroleum Hydrocarbon Contaminated Sites Based on Degradation Pathways." International Journal of Environmental Research and Public Health 18, no. 16 (August 20, 2021): 8794. http://dx.doi.org/10.3390/ijerph18168794.

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This paper analyzed the degradation pathways of petroleum hydrocarbon degradation bacteria, screened the main degradation pathways, and found the petroleum hydrocarbon degradation enzymes corresponding to each step of the degradation pathway. Through the Copeland method, the best inoculation program of petroleum hydrocarbon degradation bacteria in a polluted site was selected as follows: single oxygenation path was dominated by Streptomyces avermitilis, hydroxylation path was dominated by Methylosinus trichosporium OB3b, secondary oxygenation path was dominated by Pseudomonas aeruginosa, secondary hydroxylation path was dominated by Methylococcus capsulatus, double oxygenation path was dominated by Acinetobacter baylyi ADP1, hydrolysis path was dominated by Rhodococcus erythropolis, and CoA path was dominated by Geobacter metallireducens GS-15 to repair petroleum hydrocarbon contaminated sites. The Copeland method score for this solution is 22, which is the highest among the 375 solutions designed in this paper, indicating that it has the best degradation effect. Meanwhile, we verified its effect by the Cdocker method, and the Cdocker energy of this solution is −285.811 kcal/mol, which has the highest absolute value. Among the inoculation programs of the top 13 petroleum hydrocarbon degradation bacteria, the effect of the best inoculation program of petroleum hydrocarbon degradation bacteria was 18% higher than that of the 13th group, verifying that this solution has the best overall degradation effect. The inoculation program of petroleum hydrocarbon degradation bacteria designed in this paper considered the main pathways of petroleum hydrocarbon pollutant degradation, especially highlighting the degradability of petroleum hydrocarbon intermediate degradation products, and enriching the theoretical program of microbial remediation of petroleum hydrocarbon contaminated sites.
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3

Sun, Yanying, Fei Liu, Honghan Chen, and Wei He. "Degradation of petroleum contaminants in oil." Chinese Journal of Geochemistry 25, S1 (March 2006): 126–27. http://dx.doi.org/10.1007/bf02839982.

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4

Okpokwasili, G. C., and S. C. Amanchukwu. "Petroleum hydrocarbon degradation by Candida species." Environment International 14, no. 3 (January 1988): 243–47. http://dx.doi.org/10.1016/0160-4120(88)90145-6.

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5

Sun, Xiao Nan, An Ping Liu, Wen Ting Sun, and Shu Chang Jin. "The Remedial Effect of the Decomposing Bacteria on Different Petroleum Hydrocarbon Contamination." Advanced Materials Research 414 (December 2011): 88–92. http://dx.doi.org/10.4028/www.scientific.net/amr.414.88.

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Petroleum contamination has become one of the major soil contaminations. Aiming at petroleum hydrocarbon contamination, the multi-group opposite experiments is set; this paper use some petroleum hydrocarbon-decomposing bacteria to remedy the soil contaminated by different carbon chain petroleum hydrocarbons. Compare and study the remedial results, and study the growth of the bacteria in the decomposing process. The Study shows that the degradation rate of the bacteria to short-chain petroleum hydrocarbons is relatively high; Within 40 days without nutrient substance, degradation rate of bacteria to gasoline and diesel is 80%, degradation rate of bacteria to aromatics and lubricants is 50%, the trend of bacteria’s growth curve and the degradation rate curve of each component are approximate.
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6

Li, Chun Rong, Abao Wei, and Tao Chen. "Phytoremediation of Petroleum-Contaminated Soil." Advanced Materials Research 356-360 (October 2011): 2737–40. http://dx.doi.org/10.4028/www.scientific.net/amr.356-360.2737.

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Corn, sunflower and alfalfa were taken as remediation plants. Their phytoremediation and degradation kinetic of petroleum were investigated under field experiment. The results indicated that petroleum degradation rates of corn, sunflower and alfalfa remediation areas reached 42.5%, 46.4% and 44.7% after 150 days of remediation, which were increased by 100.5%, 118.9% and 110.8% compared with that in control area, respectively. Petroleum degradation rates of sunflower remediation areas﹥alfalfa remediation areas’﹥corn remediation areas’, whose half-lifes were 165d, 182d and 193d, respectively, which were decreased by 297d, 279d and 269d compared with that in control area, respectively. The remediation effects of corn, sunflower and alfalfa were obvious.
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7

Xu, Hui, Wen Jun Xie, and Zhao Hua Lu. "Petroleum Contaminated Soil Remediation Using Six Wild Plant Species in the Yellow River Delta." Applied Mechanics and Materials 246-247 (December 2012): 598–601. http://dx.doi.org/10.4028/www.scientific.net/amm.246-247.598.

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The tolerance and remediation efficiency of six local wild plant species in petroleum-contaminated soils in the Yellow River Delta were conducted at three contaminated levels, i.e., uncontaminated soil (control), soil contamination by petroleum at 1.48% (w/w, TI), and soil contamination by petroleum at 2.96% (w/w, TII). After 60 days, six plant species showed different petroleum contamination tolerance and degradation capability in soil. The degradation ability of Setaria viridis, Alopecurus pratensis and Echinochloa crusgalli(L) Beauv was significantly higher than that of Festuca elata, Eleusine indica (P<0.05). Suaeda salsa had the least degradation ability. Plant had the high ability to degrade petroleum in the weak pollution soil, which might be due to the low re-straining effect on plant growth. Based on their petroleum contamination tolerance and removal ef-ficiency, we suggest Alopecurus pratensis, Setaria viridis, Echinochloa crusgalli (L.) Beauv and Festuca elata are suitable for petroleum-contaminated soil remediation in the Yellow River Delta.
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8

Huang, Shengmao, Haiwen Han, Xiuren Li, Dehai Song, Wenqi Shi, Shufang Zhang, and Xianqing Lv. "Inversion of the Degradation Coefficient of Petroleum Hydrocarbon Pollutants in Laizhou Bay." Journal of Marine Science and Engineering 9, no. 6 (June 13, 2021): 655. http://dx.doi.org/10.3390/jmse9060655.

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When petroleum hydrocarbon pollutants enter the ocean, besides the migration under hydrodynamic constraints, their degradation due to environmental conditions also occurs. However, available observations are usually spatiotemporally disperse, which makes it difficult to study the degradation characteristics of pollutants. In this paper, a model of transport and degradation is used to estimate the degradation coefficient of petroleum hydrocarbon pollutants with the adjoint method. Firstly, the results of a comprehensive physical–chemical–biological test of the degradation of petroleum hydrocarbon pollutants in Laizhou Bay provide a reference for setting the degradation coefficient on the time scale. In ideal twin experiments, the mean absolute errors between observations and simulation results obtain an obvious reduction, and the given distributions can be inverted effectively, demonstrating the feasibility of the model. In a practical experiment, the actual distribution of petroleum hydrocarbon pollutants in Laizhou Bay is simulated, and the simulation results are in good agreement with the observed ones. Meanwhile, the spatial distribution of the degradation coefficient is inverted, making the simulation results closer to the actual observations.
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9

Aitkeldiyeva, S. A., E. R. Faizulina, L. G. Tatarkina, M. B. Alimzhanova, S. T. Daugaliyeva, О. N. Auezova, A. V. Alimbetova, G. A. Spankulova, and A. K. Sadanov. "DEGRADATION OF PETROLEUM HYDROCARBONS WITH THERMOTOLERANT MICROORGANISMS." Rasayan Journal of Chemistry 13, no. 02 (2020): 1271–82. http://dx.doi.org/10.31788/rjc.2020.1325580.

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10

Lustosa, Mayara A., Jorge A. López, Karla C. Santos Freire, Francine F. Padilha, María Lucila Hernández-Macedo, and Rebeca Y. Cabrera-Padilla. "Petroleum hydrocarbon degradation by isolated mangrove bacteria." Revista peruana de Biología 25, no. 4 (December 7, 2018): 441. http://dx.doi.org/10.15381/rpb.v25i4.15537.

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Los hidrocarburos de petróleo representan un problema mundial, pues su acumulación promueve un serio impacto ambiental. Así, el uso de microorganismos, por ejemplo los de la microbiota de manglares, como agentes degradadores de diversas fuentes de carbono, es poco explotado en procesos de remediación ambiental. Así, este estudio evaluó in vitro el potencial degradador de bacterias aisladas de sedimento de manglar en la degradación de hidrocarburos. El análisis genético usando el marcador 16S rRNA reveló secuencias íntimamente relacionadas (99%) con Proteobacterium, Pseudomonas y Exiguobacterium. Los resultados mostraron el crecimiento de bacterias en medio salino mineral (MSM) conteniendo petróleo o diesel al 1%, como fuentes de carbono. Este crecimiento, determinado por densidad óptica (DO) a 595 nm durante 15 días, con toma de muestras a cada 48 h, indicó la matabolización de hidrocarburos. Sin embargo, las bacterias fueron más eficientes en degradarlos. Por lo tanto, los resultados muestran la potencial aplicación de las bacterias en procesos de biorremediación por su capacidad metabólica y adaptativa de crecimiento usando hidrocarburos.
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11

Lundaa, Tserennyam, Stephanie Hummelberger, Andreas P. Loibner, and Kerstin E. Scherr. "Petroleum hydrocarbon degradation by different methanogenic consortia." Journal of Biotechnology 150 (November 2010): 256. http://dx.doi.org/10.1016/j.jbiotec.2010.09.142.

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12

Jadhav, Supriya, Sameer Sharma, and Sibi G. "Microbial Degradation of Petroleum Hydrocarbons and Factors Influencing the Degradation Process." Bioprocess Engineering 3, no. 2 (2019): 6. http://dx.doi.org/10.11648/j.be.20190302.12.

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13

Zhang, Z. Z., S. M. Su, Y. J. Luo, and M. Lu. "Improvement of natural microbial remediation of petroleum-polluted soil using graminaceous plants." Water Science and Technology 59, no. 5 (March 1, 2009): 1025–35. http://dx.doi.org/10.2166/wst.2009.081.

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A 150-day pot experiment was conducted with graminaceous plants grown in natural soil contaminated with petroleum. The relationships among microbial activity, dehydrogenase activity, catalase activity, soil moisture, and the petroleum degradation rate were analyzed. All three plants accelerated the degradation of petroleum compared with unplanted soil. Plant roots improved the soil moisture by about 5% (from 15% in unplanted soil to 20% in soil containing plant roots), and the number of microorganisms in the rhizosphere increased by more than three orders of magnitude. The induction of the rhizosphere environment and the intimidation of the petroleum changed the abundance and activity of the microorganisms. Dehydrogenase activity in the rhizosphere was 1.54 to 1.87 times the value in the unplanted soil, but catalase activity was 0.90 to 0.93 times the value in unplanted soil. The petroleum degradation rates in the rhizosphere were 2.33 to 3.19 times higher than in the unplanted soil. The effect of rhizosphere degradation clearly changed the hydrocarbon composition, increasing the degradation of alkane hydrocarbons with low and moderate carbon contents. The rhizosphere environment promoted degradation of the high-carbon-content hydrocarbons into low-carbon-content hydrocarbons. At the same time, the Pr/nC17, Ph/nC18, and Pr/Ph values increased by 0.99 and 2.69 units, and decreased by 1.25 units, respectively, compared with the undegraded oil. The plants also accelerated the isomerization of alkane hydrocarbons.
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14

Fındık, Serap. "Treatment of petroleum refinery effluent using ultrasonic irradiation." Polish Journal of Chemical Technology 20, no. 4 (December 1, 2018): 20–25. http://dx.doi.org/10.2478/pjct-2018-0049.

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Abstract Ultrasonic irradiation is one of the advanced oxidation methods used in wastewater treatment. In this study, ultrasonic treatment of petroleum refinery effluent was examined. An ultrasonic homogenizator with a 20 kHz frequency and an ultrasonic bath with a 42 kHz frequency were used as a source for ultrasound. The effects of parameters such as ZnO amount, ozone saturation time, and type of ultrasound source on the degradation of petroleum refinery effluent were investigated. The degradation of petroleum refinery effluent was measured as a change in initial chemical oxygen demand (COD) and with time. According to the results, degradation increased with the addition of ZnO in an ultrasonic probe. There was also a positive effect of ozone saturation before sonication then applying ultrasound on the degradation for an ultrasonic probe. It was observed that there was no positive effect of ZnO addition and ozone saturation on degradation for an ultrasonic bath.
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15

Wu, Chun Xu, Hong Tao Zhang, Bei Hai Zhou, Lei Gao, Hao Deng, Xuan Xu, and Yu Shuang Wang. "Optimized Environmental Conditions for Petroleum Degradation of Strain B1 by Orthogonal Test." Advanced Materials Research 393-395 (November 2011): 1308–12. http://dx.doi.org/10.4028/www.scientific.net/amr.393-395.1308.

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A strain B1 belonging to Pseudomonas aeruginosa with high petroleum-degrading efficiency was isolated from Dagang oil-field wastewater treatment plant. More than 80% petroleum-degrading efficiency was obtained at 32°C, 200 r/min. According to the orthogonal test, the environmental impact factors influencing petroleum-degrading efficiency of the strain were investigated. The optimum environmental conditions for petroleum degradation were as follows: temperature was 40 °C, pH 8.0, oil inoculation 1 mL, nitrogen content 0.05 g. And the biodegradation efficiency of the strain reached 86.1%.
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16

Gomes, R. F., Gustavo de Castro Xavier, F. A. J. Saboya, P. C. A. Maia, J. Alexandre, and L. G. Pedroti. "Degradation of Red Ceramic with Incorporated of Petroleum Waste." Materials Science Forum 798-799 (June 2014): 257–62. http://dx.doi.org/10.4028/www.scientific.net/msf.798-799.257.

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The red ceramic pieces Campos-RJ degrade easily when they are in the built environment. With the intention to reduce the degradation in these pieces, was introduced petroleum coke in red ceramic. Petroleum coke is a solid fossil fuel, black in color and about granular form. With percentage of 0%, 0.5%, 1% and 1.5% clay added to the coke were obtained is prismatic ceramic samples. After drying at 110 ° C, fired at 800°C and 900°C and carried out at accelerated degradation in the laboratory by degradation equipment, obtaining the technological properties and Weibull distributions before and after degradation in the samples. The 900°C gave the highest Weibull modulus (m = 12.50) compared with the firing pieces at 800°C. The results show that the incorporation of 0.5% of petroleum coke improves the Weibull modulus of the degraded material.
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17

Kaplan, Christopher W., and Christopher L. Kitts. "Bacterial Succession in a Petroleum Land Treatment Unit." Applied and Environmental Microbiology 70, no. 3 (March 2004): 1777–86. http://dx.doi.org/10.1128/aem.70.3.1777-1786.2004.

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ABSTRACT Bacterial community dynamics were investigated in a land treatment unit (LTU) established at a site contaminated with highly weathered petroleum hydrocarbons in the C10 to C32 range. The treatment plot, 3,000 cubic yards of soil, was supplemented with nutrients and monitored weekly for total petroleum hydrocarbons (TPH), soil water content, nutrient levels, and aerobic heterotrophic bacterial counts. Weekly soil samples were analyzed with 16S rRNA gene terminal restriction fragment (TRF) analysis to monitor bacterial community structure and dynamics during bioremediation. TPH degradation was rapid during the first 3 weeks and slowed for the remainder of the 24-week project. A sharp increase in plate counts was reported during the first 3 weeks, indicating an increase in biomass associated with petroleum degradation. Principal components analysis of TRF patterns revealed a series of sample clusters describing bacterial succession during the study. The largest shifts in bacterial community structure began as the TPH degradation rate slowed and the bacterial cell counts decreased. For the purpose of analyzing bacterial dynamics, phylotypes were generated by associating TRFs from three enzyme digests with 16S rRNA gene clones. Two phylotypes associated with Flavobacterium and Pseudomonas were dominant in TRF patterns from samples during rapid TPH degradation. After the TPH degradation rate slowed, four other phylotypes gained dominance in the community while Flavobacterium and Pseudomonas phylotypes decreased in abundance. These data suggest that specific phylotypes of bacteria were associated with the different phases of petroleum degradation in the LTU.
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18

Song, Xue Ying, Ru Jing Liang, Yu Shuang Li, Xin Xin Li, and Xiao Jun Hu. "Composting Study of Petroleum Contaminated Soil." Advanced Materials Research 864-867 (December 2013): 67–70. http://dx.doi.org/10.4028/www.scientific.net/amr.864-867.67.

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Composting has been shown to be an effective bioremediation technique for the treatment of hydrocarbon-contaminated soil. In this research, the major objective of this research was to find the appropriate mix ratio of organic amendments for enhancing the degradation of petroleum hydrocarbons during diesel oil contaminated soil composting. The spent mushroom was added as an amendment for supplementing organic matter for composting of contaminated soil. The volumn ratios of contaminated soil to organic amendments were 1:1, 1.5:1 and 2:1. Target contaminant of this research was diesel oil, which was spiked at 16240 mg/kg sample on a dry weight basis. The degradation of diesel oil was significantly enhanced by the addition of these organic amendments relative to straight soil control. Degradation rates of total petroleum hydrocarbons (TPH) were the greatest at the ratio of 1:1 of contaminated soil to organic amendments on the volumn ratio. The abiotic loss of TPH was only about 6.83% of initial TPH.
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19

Daher, Elias. "Photocatalytic degradation of phenolic effluents in petroleum refineries." International Journal of E-Learning and Educational Technologies in the Digital Media 5, no. 1 (2019): 22–29. http://dx.doi.org/10.17781/p002565.

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20

Das, Nilanjana, and Preethy Chandran. "Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview." Biotechnology Research International 2011 (September 13, 2011): 1–13. http://dx.doi.org/10.4061/2011/941810.

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One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products are of particular concern in the environment. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. Currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. Mechanical and chemical methods generally used to remove hydrocarbons from contaminated sites have limited effectiveness and can be expensive. Bioremediation is the promising technology for the treatment of these contaminated sites since it is cost-effective and will lead to complete mineralization. Bioremediation functions basically on biodegradation, which may refer to complete mineralization of organic contaminants into carbon dioxide, water, inorganic compounds, and cell protein or transformation of complex organic contaminants to other simpler organic compounds by biological agents like microorganisms. Many indigenous microorganisms in water and soil are capable of degrading hydrocarbon contaminants. This paper presents an updated overview of petroleum hydrocarbon degradation by microorganisms under different ecosystems.
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21

Andrade, Luana dos Santos, Wilson Aparecido Parejo Calvo, Ivone Mulako Sato, and Celina Lopes Duarte. "Petroleum and diesel sulfur degradation under gamma radiation." Radiation Physics and Chemistry 115 (October 2015): 196–201. http://dx.doi.org/10.1016/j.radphyschem.2014.09.015.

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22

Li, Hong, Xiaokang Li, Tao Yu, Feifei Wang, and Chengtun Qu. "Study on Extreme Microbial Degradation of Petroleum Hydrocarbons." IOP Conference Series: Materials Science and Engineering 484 (March 19, 2019): 012040. http://dx.doi.org/10.1088/1757-899x/484/1/012040.

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23

Alizadeh Fard, Mohammad, Behnoush Aminzadeh, and Hossein Vahidi. "Degradation of petroleum aromatic hydrocarbons using TiO2nanopowder film." Environmental Technology 34, no. 9 (May 2013): 1183–90. http://dx.doi.org/10.1080/09593330.2012.743592.

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24

Kruse, Birger, Bjørn K. Jensen, Svend K. Jensen, and Kurt Jensen. "Degradation of a petroleum hydrocarbon in coastal sediments." Ophelia 26, no. 1 (December 31, 1986): 285–92. http://dx.doi.org/10.1080/00785326.1986.10421994.

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25

Jamroz, T. "Degradation of petroleum in soil by biological methods." International Biodeterioration & Biodegradation 37, no. 3-4 (January 1996): 250–51. http://dx.doi.org/10.1016/0964-8305(96)88310-7.

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26

Eze, Michael O. "Metagenome Analysis of a Hydrocarbon-Degrading Bacterial Consortium Reveals the Specific Roles of BTEX Biodegraders." Genes 12, no. 1 (January 14, 2021): 98. http://dx.doi.org/10.3390/genes12010098.

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Environmental contamination by petroleum hydrocarbons is of concern due to the carcinogenicity and neurotoxicity of these compounds. Successful bioremediation of organic contaminants requires bacterial populations with degradative capacity for these contaminants. Through successive enrichment of microorganisms from a petroleum-contaminated soil using diesel fuel as the sole carbon and energy source, we successfully isolated a bacterial consortium that can degrade diesel fuel hydrocarbons. Metagenome analysis revealed the specific roles of different microbial populations involved in the degradation of benzene, toluene, ethylbenzene and xylene (BTEX), and the metabolic pathways involved in these reactions. One hundred and five putative coding DNA sequences were identified as responsible for both the activation of BTEX and central metabolism (ring-cleavage) of catechol and alkylcatechols during BTEX degradation. The majority of the Coding DNA sequences (CDSs) were affiliated to Acidocella, which was also the dominant bacterial genus in the consortium. The inoculation of diesel fuel contaminated soils with the consortium resulted in approximately 70% hydrocarbon biodegradation, indicating the potential of the consortium for environmental remediation of petroleum hydrocarbons.
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27

Sutiknowati, Lies Indah. "BIOREMEDIATION STUDY: HYDROCARBON DEGRADING BACTERIA." Marine Research in Indonesia 32, no. 2 (May 12, 2018): 95–101. http://dx.doi.org/10.14203/mri.v32i2.442.

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Many microorganisms capable of degrading petroleum components have been isolated and few of them seem to be important for petroleum biodegradation in natural environments. To identify the bacteria that play a major role in degradation of petroleum polynuclear aromatic hydrocarbons (PAHs), bacteria were enriched from seawater by using Naphthalene, Phenanthrene, Trichlorodibenzofuran and Benzo[a]pyrene as a carbon and energy source. The result of study that members of the genus Alcanivorax and Thalassospira became predominant in the enrichment cultures. The strains isolated in this study could grow on crude oil and degraded PAH components of crude oil. The number of cells increased to 8.1x106 cells g-1 after 14 days in subculture. PAH degradation proceeded parallel with the growth of bacteria cells. This observation which has been conducted in Marine Biotechnology Institute, Kamaishi, Iwate-ken, Japan suggests that Alcanivorax and Thalassospira play an important role in the degradation of petroleum PAHs in marine environment.
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28

Guo, Ping, Jian Guo Lin, Bin Xia Cao, and Na Ta. "Isolation of Petroleum Hydrocarbon Degrading Bacteria from the Liaohe Estuary at Cold Climate." Advanced Materials Research 1092-1093 (March 2015): 878–81. http://dx.doi.org/10.4028/www.scientific.net/amr.1092-1093.878.

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Two cold-tolerant petroleum hydrocarbon degrading bacteria strain named CHD1 and CHD2 were isolated from oil-contaminated soil at cold climate. The isolated strains were able use diesel oil as sole carbon. The petroleum hydrocarbon degradation rate was analyzed using UV-spectrometry-based methods. The results showed that the diesel oil degradation rate of CHD1 and CHD2 were 22% and 25%, respectively.
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29

Kumari, Babita, Manvi Singh, Pankaj Kumar Srivastava, and S. N. Singh. "Degradation of Petroleum Sludge in Soil by Bacterial-Fungal Co-Culture in Presence of Organic and Inorganic Stimulants." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 5, no. 03 (July 31, 2019): 155–64. http://dx.doi.org/10.18811/ijpen.v5i03.3.

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A microcosmic study was carried out for degradation of petroleum sludge [4% (w/w) in soil] in presence of constructed microbial consortium of three bacterial strains i.e., Pseudomonas sp. BP10, Acinetobacter sp. PSM11 and Rhodococcus sp. NJ2 and two fungal strains Panicillium oxalicum PS10 and Curvularia verruculosa PS8, isolated from different petroleum hydrocarbon contaminated sites) supplemented with vermicompost and inorganic fertilizer as biostimulants. After six months of incubation, the maximum degradation of TPH from petroleum sludge was recorded as high as 80% in the presence of combination of inorganic and organic fertilizer and microbial consortium while only 33% degradation was attributed by native organisms and abiotic factors. Enhancement (%) in degradation rate of TPH due to addition of vermicompost, inorganic fertilizer and microbial consortium in separation and combination was recorded as 57%, 13%, 35% and 139%, respectively. Besides the enhancement in specific growth rate of soil microbes due to addition of nutrient, bioaugmentation of this constructed microbial consortium also boost the total bacterial and fungal strains present in petroleum sludge contaminated soil. Catabolic enzymes played an important role in degradation and maximum induction of enzymes likes catechol 1, 2 dioxygenase, catechol 2, 3 dioxygenase, catalase, laccase and dehydrogenase activity were recorded 223.89 μ mol g-1, 323.83 μ mol g-1, 0.714 μ mol H2O2 g-1, 0.623 μ mol g-1 and 3.4 μg g-1 h-1, respectively.
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30

Weiner, Jonathan M., and Derek R. Lovley. "Rapid Benzene Degradation in Methanogenic Sediments from a Petroleum-Contaminated Aquifer." Applied and Environmental Microbiology 64, no. 5 (May 1, 1998): 1937–39. http://dx.doi.org/10.1128/aem.64.5.1937-1939.1998.

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ABSTRACT In methanogenic sediments from a petroleum-contaminated aquifer, [14C]benzene was converted to14CH4 and 14CO2 without an apparent lag. Phenol, acetate, and propionate were intermediates in benzene mineralization. These results suggest that alternative electron acceptors need not be available for there to be significant natural attenuation of benzene in some petroleum-contaminated aquifers.
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31

Atlas, Ronald M. "Fate of Petroleum Pollutants in Arctic Ecosystems." Water Science and Technology 18, no. 2 (February 1, 1986): 59–67. http://dx.doi.org/10.2166/wst.1986.0016.

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Both experimental oil release field studies, in Arctic tundra, freshwater, and marine ecosystems, and follow-up studies after Arctic and subarctic oil spillages indicate long persistence times for hydrocarbon contaminants and slow rates of microbial biodegradation. The slow rates of petroleum biodegradation in Arctic ecosystems are not due to a lack of indigenous hydrocarbon-degrading microorganisms since virtually all Arctic ecosystems contain numbers of naturally occurring populations of hydrocarbon-degrading microorganisms, and generally numbers of hydrocarbon degraders increase following addition of oil. Low temperatures alone also can not explain the limited rates of hydrocarbon biodegradation. Rather the limitation to microbial degradation of petroleum hydrocarbons in Arctic ecosystems appears to be due to the combination of several factors, including the availability of nitrogen, phosphorus, and oxygen. Although the potential for hydrocarbon degradation exists, the actual rates of hydrocarbon biodegradation in Arctic ecosystems are slow; microbial hydrocarbon degradation can decontaminate Arctic ecosystems but the time frame after a major spillage will be decades rather than years.
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32

Xu, Rui Dan. "Bioremediation of Petroleum Contaminated Soil." Advanced Materials Research 550-553 (July 2012): 1248–52. http://dx.doi.org/10.4028/www.scientific.net/amr.550-553.1248.

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Two kinds of polyacrylamide(HPAM)-degrading bacteria S1, S2, which can use HPAM as only nitrogen source and the sole carbon source, were isolated from petroleum-contaminated soil of Daqing Oilfield. The bioremediation for treating petroleum contaminated soil by immobilized microorganisms can improve the effect on biodegradation for pollutants in oil fields and reduce the loss of bacteria. The degradation ability of five kinds of embedding immobilization methods on soil pollutant was investigated. The experimental results showed that the immobilized microbial granules, which used polyvinyl alcohols (PVA) and sodium alginate as coagulant, activated carbon as coagulant-support, exhibited good mechanical strength, operated easily, be not breakable and low cost. Experiments results showed that after treatment using this kind of immobilized microbial granules, the HPAM concentration declined from 500 mg•L-1 to 102 mg•L-1 in 48 hours. The degradation rate of HPAM reached 79.6%. At the same time crude oil content decreased from 733.21 mg•L-1 to 9.5 mg•L-1. These immobilized microbial granules can remove 98.7% oil from the petroleum-contaminated soil in 48 hours.
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33

Sui, Xin, Xuemei Wang, Yuhuan Li, and Hongbing Ji. "Remediation of Petroleum-Contaminated Soils with Microbial and Microbial Combined Methods: Advances, Mechanisms, and Challenges." Sustainability 13, no. 16 (August 18, 2021): 9267. http://dx.doi.org/10.3390/su13169267.

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The petroleum industry’s development has been supported by the demand for petroleum and its by-products. During extraction and transportation, however, oil will leak into the soil, destroying the structure and quality of the soil and even harming the health of plants and humans. Scientists are researching and developing remediation techniques to repair and re-control the afflicted environment due to the health risks and social implications of petroleum hydrocarbon contamination. Remediation of soil contamination produced by petroleum hydrocarbons, on the other hand, is a difficult and time-consuming job. Microbial remediation is a focus for soil remediation because of its convenience of use, lack of secondary contamination, and low cost. This review lists the types and capacities of microorganisms that have been investigated to degrade petroleum hydrocarbons. However, investigations have revealed that a single microbial remediation faces difficulties, such as inconsistent remediation effects and substantial environmental consequences. It is necessary to understand the composition and source of pollutants, the metabolic genes and pathways of microbial degradation of petroleum pollutants, and the internal and external aspects that influence remediation in order to select the optimal remediation treatment strategy. This review compares the degradation abilities of microbial–physical, chemical, and other combination remediation methods, and highlights the degradation capabilities and processes of the greatest microbe-biochar, microbe–nutrition, and microbe–plant technologies. This helps in evaluating and forecasting the chemical behavior of contaminants with both short- and long-term consequences. Although there are integrated remediation strategies for the removal of petroleum hydrocarbons, practical remediation remains difficult. The sources and quantities of petroleum pollutants, as well as their impacts on soil, plants, and humans, are discussed in this article. Following that, the focus shifted to the microbiological technique of degrading petroleum pollutants and the mechanism of the combined microbial method. Finally, the limitations of existing integrated microbiological techniques are highlighted.
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34

Akintayo, Cecilia O., Omolola H. Aremu, Wilfred N. Igboama, Simphiwe M. Nelana, and Olushola S. Ayanda. "Performance Evaluation of Ultra-Violet Light and Iron Oxide Nanoparticles for the Treatment of Synthetic Petroleum Wastewater: Kinetics of COD Removal." Materials 14, no. 17 (September 2, 2021): 5012. http://dx.doi.org/10.3390/ma14175012.

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In this study, the use of ultra-violet (UV) light with or without iron oxide nanoparticles (IONPs) for the degradation of synthetic petroleum wastewater was investigated. The IONPs was synthesised by sodium borohydride reduction of ferric chloride solution and was characterised by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectrometry (FTIR), x-ray fluorescence spectrophotometry (XRF), and energy dispersive spectroscopy (EDS). The amount of degradation was evaluated by chemical oxygen demand (COD) determination. Experimental results show that the COD removal from synthetic petroleum wastewater by IONPs/UV system was more effective than they were independently. The combination of UV light at a wavelength of 254 nm, pH of 8, and 1.0 g of IONPs resulted in COD removal from 10.5% up to 95.5%. The photocatalytic degradation of synthetic petroleum wastewater is about 1.3–2.0 times faster in comparison to UV light only. The removal of COD from synthetic petroleum wastewater by UV light and IONPs follows the pseudo-first-order kinetic model with rate constant k ranging from 0.0133 min−1 to 0.0269 min−1. Consequently, this study has shown that the use of UV light in the presence of IONPs is favourable and effective for the removal of organic pollutants from petroleum refinery wastewater.
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35

UZAIR, B., M. MUNIR, S. TASSADAQ, S. KHAN, and B. A. KHAN. "BACTERIA-MEDIATED DEGRADATION OF PETROLEUM HYDROCARBON CONTAMINANTS: AN OVERVIEW." Latin American Applied Research - An international journal 46, no. 4 (October 31, 2016): 139–46. http://dx.doi.org/10.52292/j.laar.2016.345.

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One of the major environmental problems is hydrocarbon pollution. Hydrocarbons are mostly the result of petroleum based activities. Anthropogenic activities, natural seepage and accidental spills are of particular interest in the environmental quality. The health effects of these chemicals are widely known. In the hour of alarming pollution by these hydrocarbons, a newer, cheaper, and safer technology is needed for cleanup, moving beyond the conventional mechanical and chemical methods, which are not only expensive but ineffective also. Bioremediation is a promising technology, functioning on complete mineralization of contaminants by the diverse metabolic processes owned by microorganisms. Many indigenous and genetically modified bacteria are capable of crude oil degradation. This paper presents an updated overview of petroleum hydrocarbon degradation by bacteria.
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36

Mukherjee, Souryadeep, Arijit De, Nirmal Kumar Sarkar, and Nimai Chandra Saha. "Aerobic Degradation of Benzene by Escherichia spp. from Petroleum-contaminated Sites in Kolkata, West Bengal, India." Journal of Pure and Applied Microbiology 13, no. 4 (December 30, 2019): 2353–62. http://dx.doi.org/10.22207/jpam.13.4.51.

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37

Goveas, Louella Concepta, Amrutha Krishna, Ananya Salian, Jenishia Menezes, Melita Alva, Bharath Basavapattan, and Shyama Prasad Sajankila. "Isolation and Characterization of Bacteria from Refinery Effluent for Degradation of Petroleum Crude Oil in Seawater." Journal of Pure and Applied Microbiology 14, no. 1 (March 31, 2020): 473–84. http://dx.doi.org/10.22207/jpam.14.1.49.

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38

Kim, Sung Un, Yong Gyun Kim, Sang Mong Lee, Hyean Cheal Park, Keun Ki Kim, Hong Joo Son, Yong Dong Noh, and Chang Oh Hong. "The Effect of Compost Application on Degradation of Total Petroleum Hydrocarbon in Petroleum-Contaminated Soil." Korean Journal of Environmental Agriculture 34, no. 4 (December 31, 2015): 268–73. http://dx.doi.org/10.5338/kjea.2015.34.4.45.

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39

Tsodikov, M. V., M. A. Perederii, A. V. Chistyakov, G. I. Konstantinov, Kh M. Kadiev, and S. N. Khadzhiev. "High-speed degradation of sorbed petroleum residues and pollutants." Solid Fuel Chemistry 46, no. 2 (April 2012): 121–27. http://dx.doi.org/10.3103/s0361521912020115.

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40

Xilai, ZHENG, WANG Bingchen, LI Yuying, and XIA Wenxiang. "Degradation Kinetics of Petroleum Contaminants in Soil-Water Systems." Acta Geologica Sinica - English Edition 78, no. 3 (September 7, 2010): 825–28. http://dx.doi.org/10.1111/j.1755-6724.2004.tb00202.x.

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41

Fedorak, P. M., J. D. Payzant, D. S. Montgomery, and D. W. Westlake. "Microbial degradation of n-alkyl tetrahydrothiophenes found in petroleum." Applied and Environmental Microbiology 54, no. 5 (1988): 1243–48. http://dx.doi.org/10.1128/aem.54.5.1243-1248.1988.

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42

Stepnowski, P., E. M. Siedlecka, P. Behrend, and B. Jastorff. "Enhanced photo-degradation of contaminants in petroleum refinery wastewater." Water Research 36, no. 9 (May 2002): 2167–72. http://dx.doi.org/10.1016/s0043-1354(01)00450-x.

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43

Antić, Mališa P., Branimir Jovancicevic, Miroslav M. Vrvić, and Jan Schwarzbauer. "Petroleum Pollutant Degradation by Surface Water Microorganisms (8 pp)." Environmental Science and Pollution Research - International 13, no. 5 (March 29, 2006): 320–27. http://dx.doi.org/10.1065/espr2006.03.296.

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44

Cho, Il-Hyoung, Lee-Hyung Kim, Kyung-Duk Zoh, Jae-Hong Park, and Hyun-Yong Kim. "Solar photocatalytic degradation of groundwater contaminated with petroleum hydrocarbons." Environmental Progress 25, no. 2 (2006): 99–109. http://dx.doi.org/10.1002/ep.10124.

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45

Su, Zeng Jian, Min Li, and Yu Xiu Zhang. "Degradation Microorganisms Filtration of Petroleum Hydrocarbon in Tropic Ocean." Advanced Materials Research 1010-1012 (August 2014): 737–41. http://dx.doi.org/10.4028/www.scientific.net/amr.1010-1012.737.

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Six oil degradation strains were obtained from the tropic ocean of Hainan province using diesel oil as the sole carbon source in the research, which aim is to prevent and restore the ocean oil pollution at present and in future. The degradation rate of diesel oil by these strains was tested and three stains were filtrated as the dominant bacteria from Soh7, Soh11, Soh23, Soh26, Soh38 and Soh53 which were Soh7, Soh11 and Soh53. The 15d DR were 40.6%,31.3%,37.1% and 25d DR were 51.2%, 40.3%, 47.8% (TDR were 63.1%, 54.9%, 60.6%) separately under 24°C. Based on the morphological, physiological and biochemical test results, the Soh7 was identified asSporolactobacillus sp.and Soh11 and Soh53 wereAcidothermus sp..
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46

Unimke, A. A., O. A. Mmuoegbulam, and O. C. Anika. "Microbial Degradation of Petroleum Hydrocarbons: Realities, Challenges and Prospects." Biotechnology Journal International 22, no. 2 (November 14, 2018): 1–10. http://dx.doi.org/10.9734/bji/2018/43957.

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47

PARKER, ERIC F., and WILLIAM D. BURGOS. "Degradation Patterns of Fresh and Aged Petroleum-Contaminated Soils." Environmental Engineering Science 16, no. 1 (January 1999): 21–29. http://dx.doi.org/10.1089/ees.1999.16.21.

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48

Almeida, K. C. S., H. Oikawa, J. Oliveira, and C. L. Duarte. "Degradation of petroleum hydrocarbons in seawater by ionizing radiation." Journal of Radioanalytical and Nuclear Chemistry 270, no. 1 (October 2006): 93–97. http://dx.doi.org/10.1007/s10967-006-0313-4.

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49

Nzila, Alexis. "Current Status of the Degradation of Aliphatic and Aromatic Petroleum Hydrocarbons by Thermophilic Microbes and Future Perspectives." International Journal of Environmental Research and Public Health 15, no. 12 (December 7, 2018): 2782. http://dx.doi.org/10.3390/ijerph15122782.

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Contamination of the environment by petroleum products is a growing concern worldwide, and strategies to remove these contaminants have been evaluated. One of these strategies is biodegradation, which consists of the use of microorganisms. Biodegradation is significantly improved by increasing the temperature of the medium, thus, the use of thermophiles, microbes that thrive in high-temperature environments, will render this process more efficient. For instance, various thermophilic enzymes have been used in industrial biotechnology because of their unique catalytic properties. Biodegradation has been extensively studied in the context of mesophilic microbes, and the mechanisms of biodegradation of aliphatic and aromatic petroleum hydrocarbons have been elucidated. However, in comparison, little work has been carried out on the biodegradation of petroleum hydrocarbons by thermophiles. In this paper, a detailed review of the degradation of petroleum hydrocarbons (both aliphatic and aromatic) by thermophiles was carried out. This work has identified the characteristics of thermophiles, and unraveled specific catabolic pathways of petroleum products that are only found with thermophiles. Gaps that limit our understanding of the activity of these microbes have also been highlighted, and, finally, different strategies that can be used to improve the efficiency of degradation of petroleum hydrocarbons by thermophiles were proposed.
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50

Li, Dongmei, and Philip Hendry. "Microbial diversity in petroleum reservoirs." Microbiology Australia 29, no. 1 (2008): 25. http://dx.doi.org/10.1071/ma08025.

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Buried hydrocarbon deposits, such as liquid petroleum, represent an abundant source of reduced carbon for microbes. It is not surprising therefore that many organisms have adapted to an oily, anaerobic life deep underground, often at high temperatures and pressures, and that those organisms have had, and in some cases continue to have, an effect on the quality and recovery of the earth?s diminishing petroleum resources. There are three key microbial processes of interest to petroleum producers: reservoir souring, hydrocarbon degradation and microbially enhanced oil recovery (MEOR).
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