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1

Truskewycz, Adam, Taylor D. Gundry, Leadin S. Khudur, Adam Kolobaric, Mohamed Taha, Arturo Aburto-Medina, Andrew S. Ball, and Esmaeil Shahsavari. "Petroleum Hydrocarbon Contamination in Terrestrial Ecosystems—Fate and Microbial Responses." Molecules 24, no. 18 (September 19, 2019): 3400. http://dx.doi.org/10.3390/molecules24183400.

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Petroleum hydrocarbons represent the most frequent environmental contaminant. The introduction of petroleum hydrocarbons into a pristine environment immediately changes the nature of that environment, resulting in reduced ecosystem functionality. Natural attenuation represents the single, most important biological process which removes petroleum hydrocarbons from the environment. It is a process where microorganisms present at the site degrade the organic contaminants without the input of external bioremediation enhancers (i.e., electron donors, electron acceptors, other microorganisms or nutrients). So successful is this natural attenuation process that in environmental biotechnology, bioremediation has developed steadily over the past 50 years based on this natural biodegradation process. Bioremediation is recognized as the most environmentally friendly remediation approach for the removal of petroleum hydrocarbons from an environment as it does not require intensive chemical, mechanical, and costly interventions. However, it is under-utilized as a commercial remediation strategy due to incomplete hydrocarbon catabolism and lengthy remediation times when compared with rival technologies. This review aims to describe the fate of petroleum hydrocarbons in the environment and discuss their interactions with abiotic and biotic components of the environment under both aerobic and anaerobic conditions. Furthermore, the mechanisms for dealing with petroleum hydrocarbon contamination in the environment will be examined. When petroleum hydrocarbons contaminate land, they start to interact with its surrounding, including physical (dispersion), physiochemical (evaporation, dissolution, sorption), chemical (photo-oxidation, auto-oxidation), and biological (plant and microbial catabolism of hydrocarbons) interactions. As microorganism (including bacteria and fungi) play an important role in the degradation of petroleum hydrocarbons, investigations into the microbial communities within contaminated soils is essential for any bioremediation project. This review highlights the fate of petroleum hydrocarbons in tertial environments, as well as the contributions of different microbial consortia for optimum petroleum hydrocarbon bioremediation potential. The impact of high-throughput metagenomic sequencing in determining the underlying degradation mechanisms is also discussed. This knowledge will aid the development of more efficient, cost-effective commercial bioremediation technologies.
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2

Bekturova, Assemgul, Zhannur Markhametova, and Zhaksylyk Masalimov. "Plasmids Role in Survival of Acinetobacter calcoaceticus A1 Exposed to UV-Radiation and Hydrocarbons." Advanced Materials Research 905 (April 2014): 151–55. http://dx.doi.org/10.4028/www.scientific.net/amr.905.151.

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The role of plasmids in hydrocarbon-degrading bacteriaAcinetobacter calcoaceticus A1survival to UV-radiation and hydrocarbons was studied. Natural plasmids-containingA. calcoaceticus A1showed high resistance to UV-radiation.A. calcoaceticus A1showed active growth under exposed to UV-radiation for up to 30 minutes. Combined effects of UV-radiation and petroleum hydrocarbons did not considerably reduce the growth of strains. It was shown a stimulating effect of UV-radiation on the growth curves of strains ofA. calcoaceticus A1. Constructed recombinant strain (E.coli XL blueRec) showed the ability to grow on medium with addition petroleum hydrocarbons. Combined effects of UV-radiation and petroleum hydrocarbons have had a negative effect on the growth ofE.coli XL blueRec. Thus, results showed that the plasmid DNA of natural hydrocarbon-degrading bacteriaA. calcoaceticus A1may contain genes of microbial resistance to UV - radiation and petroleum hydrocarbons.
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3

Liu, Jianbo, Liming Xu, Feifei Zhu, and Shouhao Jia. "Effects of surfactants on the remediation of petroleum contaminated soil and surface hydrophobicity of petroleum hydrocarbon degrading flora." Environmental Engineering Research 26, no. 5 (September 20, 2020): 200384–0. http://dx.doi.org/10.4491/eer.2020.384.

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It has been proven that surfactants used in the remediation of petroleum hydrocarbon contaminated soil have great application potential. In this study, the effects of five surfactants (SDBS, Tween80, Tween60, rhamnolipid and TRS-1) on leaching of petroleum hydrocarbons from soil were investigated through orthogonal experiments, and petroleum hydrocarbon components were analyzed by GC/MS. The effects of surfactants on the degradation of petroleum hydrocarbon were analyzed by the changes of microbial growth curve and surface hydrophobicity. The results showed that surfactant type, temperature and surfactant concentration had significant effects on the removal rate of petroleum hydrocarbon. Tween80, rhamnolipid and TRS-1 have good bio-friendliness and a high removal rate of petroleum hydrocarbons (up to 65%), suitable for the restoration of the soil used in the experiment And Surfactants exhibited a higher removal rate for small molecules and petroleum hydrocarbons with odd carbon atoms. Surfactants have a certain modification effect on the surface of relatively hydrophilic bacteria under the initial conditions, making their surface properties develop in the direction of enhanced hydrophobicity, and the hydrophobicity has increased from less than 20% to about 40%.
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4

Fan, Li, Xianhe Gong, Quanwei Lv, Denghui Bin, and Li’Ao Wang. "Construction of Shale Gas Oil-Based Drilling Cuttings Degrading Bacterial Consortium and Their Degradation Characteristics." Microorganisms 12, no. 2 (February 2, 2024): 318. http://dx.doi.org/10.3390/microorganisms12020318.

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Oil-based drilling cuttings (OBDCs) contain petroleum hydrocarbons with complex compositions and high concentrations, which have highly carcinogenic, teratogenic, and mutagenic properties. In this study, three highly efficient petroleum hydrocarbon-degrading bacteria were screened from OBDCs of different shale gas wells in Chongqing, China, and identified as Rhodococcus sp. and Dietzia sp. Because of their ability to degrade hydrocarbons of various chain lengths, a new method was proposed for degrading petroleum hydrocarbons in shale gas OBDCs by combining different bacterial species. Results showed that the bacterial consortium, consisting of the three strains, exhibited the highest degradation rate for petroleum hydrocarbons, capable of degrading 74.38% of long-chain alkanes and 93.57% of short-chain alkanes, respectively. Moreover, the petroleum hydrocarbon degradation performance of the bacterial consortium in actual OBDCs could reach 90.60% in the optimal conditions, and the degradation kinetic process followed a first-order kinetic model. This study provides a certain technical reserve for the bioremediation of shale gas OBDCs.
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5

Yu, Haibin, Jiazhong Zang, Chunlei Guo, Bin Li, Ben Li, Xueyin Zhang, and Tiehong Chen. "Research Progress on Adsorption and Separation of Petroleum Hydrocarbon Molecules by Porous Materials." Separations 10, no. 1 (December 29, 2022): 17. http://dx.doi.org/10.3390/separations10010017.

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Petroleum is an indispensable chemical product in industrial production and daily life. The hydrocarbon molecules in petroleum are important raw materials in the organic chemical industry. The hydrocarbons currently used in industry are usually obtained by fractional distillation of petroleum, which not only consumes more energy, but has poor separation selectivity for some hydrocarbons. Adsorption separation technology has many advantages such as energy saving and high efficiency. It can adsorb and separate hydrocarbon molecules in petroleum with low energy consumption and high selectivity under mild conditions. In this paper, the research progress of adsorption and separation of hydrocarbon molecules in petroleum is reviewed, and various new catalysts and the rules of adsorption and desorption are analyzed.
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6

Kotova, V. E., Yu А. Andreev, О. А. Mikhaylenko, and I. А. Ryazantseva. "ASSESSMENT OF PETROLEUM COMPONENT CONTAMINATION OF WATER IN THE TEMERNIK RIVER AND ITS INFLUENCE ON THE DON RIVER." Ecology. Economy. Informatics.System analysis and mathematical modeling of ecological and economic systems 1, no. 6 (2021): 112–17. http://dx.doi.org/10.23885/2500-395x-2021-1-6-112-117.

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Here, we report the results of petroleum component contamination assessment of the Temernik river and the Don river. Our aim was to study the hydrocarbon group content of petroleum components in the river water. Thus, we determined the mass concentrations of chemical oxygen demand, petroleum components, and aliphatic and polycyclic aromatic hydrocarbons. In the Temernik river, the concentrations of chemical oxygen demand, petroleum components, sum of aliphatic and polycyclic aromatic hydrocarbons were 21.4–34.4 mg/L, 0.14–6.0 mg/L, 10–18 μg/L, and 0.17–2.9 μg/L, respectively. The concentrations of chemical oxygen demand, petroleum components, and benzo[a]pyrene exceeded the maximum permissible concentration by 1.4–2.3, 2.8-120, and 1.3–5.8 times, respectively. In the Don river, the concentrations of chemical oxygen demand, petroleum components, sum of aliphatic and polycyclic aromatic hydrocarbons were 18.7-29.5 mg/L, 0.08- 0.16 mg/L, 8.2-12 μg/L, and 0.03-0.13 μg/L, respectively. The Severnoe reservoir was the less contaminated part of the river. The Temernik river estuary was the most contaminated part of the river. The pollutant concentrations increased in the Don River downstream of the Temernik river estuary. Therefore, the Temernik river influences on the Don river contamination. The chemical oxygen demand, petroleum components, and polycyclic aromatic hydrocarbons had the close distribution of concentrations in the rivers. However, the aliphatic hydrocarbon concentration changed in another way. The results of the study showed that the hydrocarbon groups of petroleum components can have different sources.
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7

Alaidaroos, Bothaina A. "Advancing Eco-Sustainable Bioremediation for Hydrocarbon Contaminants: Challenges and Solutions." Processes 11, no. 10 (October 22, 2023): 3036. http://dx.doi.org/10.3390/pr11103036.

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In an era of rising population density and industrialization, the environment confronts growing challenges. Soil, agricultural land, and water bodies are becoming increasingly polluted by petroleum waste and hydrocarbons. While hydrocarbons are naturally present in crude oil, refining processes compound the complexity and toxicity of hydrocarbons. This is particularly evident in polycyclic aromatic hydrocarbons (PAHs) found in the air and soil, known for their carcinogenic, mutagenic, and teratogenic properties. In response, biodegradation emerges as an eco-friendly, cost-effective solution, especially in petroleum-contaminated settings. Biodiverse microbial communities play a pivotal role in managing hydrocarbon contamination, contingent on location, toxicity, and microbial activity. To optimize biodegradation, understanding its mechanisms is essential. This review delves into varied bioremediation techniques, degradation pathways, and the contributions of microbial activities to efficiently removing hydrocarbon pollutants. Recent research spotlights specific microorganisms like bacteria, microalgae, and fungi adept at hydrocarbon degradation, offering a contemporary perspective on petroleum hydrocarbon pollutant bioremediation. These microorganisms efficiently break down petroleum hydrocarbons, with enzymatic catalysis markedly accelerating pollutant breakdown compared to conventional methods. Given the intricate nature of hydrocarbon contamination, cooperative bacterial consortia are instrumental in effective cleanup, driven by specific genes guiding bacterial metabolism. For cost-effective and efficient removal from compromised environments, it is advisable to adopt an integrated approach that combines biostimulation and bioaugmentation.
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8

Su, Qu, Jiang Yu, Kaiqin Fang, Panyue Dong, Zheyong Li, Wuzhu Zhang, Manxia Liu, Luojing Xiang, and Junxiong Cai. "Microbial Removal of Petroleum Hydrocarbons from Contaminated Soil under Arsenic Stress." Toxics 11, no. 2 (February 1, 2023): 143. http://dx.doi.org/10.3390/toxics11020143.

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The contamination of soils with petroleum and its derivatives is a longstanding, widespread, and worsening environmental issue. However, efforts to remediate petroleum hydrocarbon-polluted soils often neglect or overlook the interference of heavy metals that often co-contaminate these soils and occur in petroleum itself. Here, we identified Acinetobacter baumannii strain JYZ-03 according to its Gram staining, oxidase reaction, biochemical tests, and FAME and 16S rDNA gene sequence analyses and determined that it has the ability to degrade petroleum hydrocarbons. It was isolated from soil contaminated by both heavy metals and petroleum hydrocarbons. Strain JYZ-03 utilized diesel oil, long-chain n-alkanes, branched alkanes, and polycyclic aromatic hydrocarbons (PAHs) as its sole carbon sources. It degraded 93.29% of the diesel oil burden in 7 days. It also had a high tolerance to heavy metal stress caused by arsenic (As). Its petroleum hydrocarbon degradation efficiency remained constant over the 0–300 mg/L As(V) range. Its optimal growth conditions were pH 7.0 and 25–30 °C, respectively, and its growth was not inhibited even by 3.0% (w/v) NaCl. Strain JYZ-03 effectively bioremediates petroleum hydrocarbon-contaminated soil in the presence of As stress. Therefore, strain JYZ-03 may be of high value in petroleum- and heavy-metal-contaminated site bioremediation.
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9

Sun, Xiao Nan, An Ping Liu, Wen Ting Sun, and Shu Chang Jin. "The Remedial Effect of the Decomposing Bacteria on Different Petroleum Hydrocarbon Contamination." Advanced Materials Research 414 (December 2011): 88–92. http://dx.doi.org/10.4028/www.scientific.net/amr.414.88.

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Petroleum contamination has become one of the major soil contaminations. Aiming at petroleum hydrocarbon contamination, the multi-group opposite experiments is set; this paper use some petroleum hydrocarbon-decomposing bacteria to remedy the soil contaminated by different carbon chain petroleum hydrocarbons. Compare and study the remedial results, and study the growth of the bacteria in the decomposing process. The Study shows that the degradation rate of the bacteria to short-chain petroleum hydrocarbons is relatively high; Within 40 days without nutrient substance, degradation rate of bacteria to gasoline and diesel is 80%, degradation rate of bacteria to aromatics and lubricants is 50%, the trend of bacteria’s growth curve and the degradation rate curve of each component are approximate.
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10

Yang, B., Q. H. Xue, C. T. Qu, C. Lu, F. F. Liu, H. Zhang, L. T. Ma, L. Qi, and Y. T. Wang. "Research Progress on in-situ Remediation of Typical Heavy Metals in Petroleum Hydrocarbon-contaminated Soil Enrichment by Plants." Nature Environment and Pollution Technology 23, no. 1 (March 1, 2024): 87–97. http://dx.doi.org/10.46488/nept.2024.v23i01.006.

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Petroleum hydrocarbon is one of the dangerous substances in the process of petroleum development, refining, processing, transportation, and production. In the related activities of the petroleum industry, the output is large, and improper treatment will cause pollution to the surrounding environment. It is an urgent problem to conduct harmless and resource treatment of petroleum hydrocarbon polluted soil. Plant enrichment, as an environmentally friendly and pollution-free technical means, has the advantages of low cost and small change to the soil environment and effectively solves the problems of excessive heavy metals in petroleum hydrocarbons through plant enrichment. In this paper, the development process of plant enrichment, remediation methods, and plant enrichment of typical heavy metals (Cd, Hg, Zn) in petroleum hydrocarbon-polluted soil were systematically introduced. Through investigation, the mechanism and influencing factors of plant enrichment of heavy metals in the presence of petroleum hydrocarbons were summarized and analyzed, and the possible development direction of plant enrichment technology in the future was prospected.
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11

Mahmud, Tasiu, Ibrahim Alhaji Sabo, Zakari Nuhu Lambu, Dauda Danlami, and Adamu Abdullahi Shehu. "Hydrocarbon Degradation Potentials of Fungi: A Review." Journal of Environmental Bioremediation and Toxicology 5, no. 1 (August 5, 2022): 50–56. http://dx.doi.org/10.54987/jebat.v5i1.681.

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One of the serious problems affecting the environment nowadays is petroleum hydrocarbon contaminations resulting from the activities in the oil and gas sector, these include: oil-spill, tank leakage, lubrication, petroleum exploitation, transportation, and services. Various techniques including mechanical and chemical methods have been employed for the bioremediation and degradation of hydrocarbons pollutants from the environments, however, some of these methods are generally expensive and may have detrimental effects on the environment, hence bioremediation is the alternative solution to hydrocarbon pollutants. Among microorganisms used in bioremediation technology nowadays, fungi are efficient, reliable, cost-effective, and environmentally friendly that can be used to cleanup and detoxify hydrocarbons contaminants from the environment viz; soil, water, and sediments. Bioremediation using fungi ensures the complete degradation and mineralization of petroleum hydrocarbon contaminants into carbon dioxide, water, inorganic compounds, and cell biomass. This review focuses on the potentials of fungi in the bioremediation of total petroleum hydrocarbons including the polycyclic aromatic hydrocarbons (PAHs). We reviewed and discussed current approaches in the bioremediation of hydrocarbon including the mechanisms of fungal bioremediation of hydrocarbon, which involves biosurfactants production and the use of fungal enzymes in the degradation of hydrocarbon pollutants. In general, fungi are more efficient and effective in the removal of hydrocarbon contaminants from the environments viz., water, soil, and sediments. However, the potentiality of fungi has not been exploited fully, hence further studies are recommended especially in the current genomic and proteomic era.
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12

Gargouri, Boutheina, Najla Mhiri, Fatma Karray, Fathi Aloui, and Sami Sayadi. "Isolation and Characterization of Hydrocarbon-Degrading Yeast Strains from Petroleum Contaminated Industrial Wastewater." BioMed Research International 2015 (2015): 1–11. http://dx.doi.org/10.1155/2015/929424.

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Two yeast strains are enriched and isolated from industrial refinery wastewater. These strains were observed for their ability to utilize several classes of petroleum hydrocarbons substrates, such asn-alkanes and aromatic hydrocarbons as a sole carbon source. Phylogenetic analysis based on the D1/D2 variable domain and the ITS-region sequences indicated that strains HC1 and HC4 were members of the generaCandidaandTrichosporon, respectively. The mechanism of hydrocarbon uptaking by yeast,Candida,andTrichosporonhas been studied by means of the kinetic analysis of hydrocarbons-degrading yeasts growth and substrate assimilation. Biodegradation capacity and biomass quantity were daily measured during twelve days by gravimetric analysis and gas chromatography coupled with mass spectrometry techniques. Removal ofn-alkanes indicated a strong ability of hydrocarbon biodegradation by the isolated yeast strains. These two strains grew on long-chainn-alkane, diesel oil, and crude oil but failed to grow on short-chainn-alkane and aromatic hydrocarbons. Growth measurement attributes of the isolates, usingn-hexadecane, diesel oil, and crude oil as substrates, showed that strain HC1 had better degradation for hydrocarbon substrates than strain HC4. In conclusion, these yeast strains can be useful for the bioremediation process and decreasing petroleum pollution in wastewater contaminated with petroleum hydrocarbons.
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13

Gurov, Yuri P., Evgeny О. Zemlianskii, Andrey G. Mozyrev, and Slavik G. Agaev. "PARAMETERS CRYSTALLIZATION PROCESSES AND SOLID PETROLEUM HYDROCARBONS DISSOLUTION." IZVESTIYA VYSSHIKH UCHEBNYKH ZAVEDENII KHIMIYA KHIMICHESKAYA TEKHNOLOGIYA 63, no. 6 (May 13, 2020): 90–94. http://dx.doi.org/10.6060/ivkkt.20206306.6181.

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In the proposed work, the experimental data on the processes of crystallization and different nature waxy hydrocarbons recrystallization in hydrocarbon solvents have been compared. T-1 technical paraffin (GOST 23683-89) with the melting point of 54 °С and ceresin-80 (GOST 2488-79) with the dropping temperature of 80 °C have been used. РТ-1 kerosene (GOST 10227-86) and de-waxed oil of fraction 420-490 °С have been used as hydrocarbon solvents. The experimental data on crystallization and recrystallization processes of paraffin wax with a melting temperature of 54 ºC and ceresin with a dropping temperature of 80 °C in kerosene and dewaxed oil are presented in this paper. It is shown that chemical structure has the main influence on the processes of crystallization and recrystallization of solid petroleum hydrocarbons. An exceedance of solid hydrocarbons solution temperatures tр above their cloud points tп has been observed which is explained by hysteretic processes. The temperature difference Δt = tр- tп depends on the solid hydrocarbons nature and their content in solvents. Wax solutions in kerosene have higher values Δt relative to ceresin solutions in kerosene, which can be explained by the difference in chemical structure of solid hydrocarbons. With the increase in solid hydrocarbons content in their solvents due to the differences in solid hydrocarbons diffusion rate, Δt decreases. The discovered regularities of solid hydrocarbons crystallization and recrystallization should be taken into account in the processes of paraffin oil production, transportation and processing.
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14

Sharma, S. S., and A. Vashishtha. "Physicochemical characterisation of petroleum hydrocarbon contaminated land of Guru Gobind Singh refinery’s peripheral area, Punjab." Environment Conservation Journal 22, no. 1&2 (June 19, 2021): 213–16. http://dx.doi.org/10.36953/ecj.2021.221230.

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Petroleum hydrocarbons are a critical environmental contaminant and pose a serious hazard to the living system as petroleum hydrocarbons are identified as carcinogenic and neurotoxic organic pollutants. Therefore, remedial methods are required to dispose of it. With a modern understanding of nature and microorganisms, bioremediation is the preferred method for soil pollution control. However, before the implementation of successful bioremediation technology, it is required to assess various physico-chemical parameters of contaminated soil. In the present study, various physico-chemical parameters, including pH, electrical conductivity, water holding capacity, organic carbon, organic matter, available nitrogen, carbonate, bicarbonate, potassium and sodium contents of the petroleum hydrocarbon contaminated soil were estimated. The results suggested a rise in all the estimated parameters for the petroleum hydrocarbon-contaminated soil.
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15

Al-Rubaye, Ameer H., Dheyaa J. Jasim, Hawzhen Fateh M. Ameen, Hazim Aziz Al-Robai, and Jawad R. Al-Assal. "The Impacts of Petroleum on Environment." IOP Conference Series: Earth and Environmental Science 1158, no. 3 (April 1, 2023): 032014. http://dx.doi.org/10.1088/1755-1315/1158/3/032014.

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Abstract Petroleum is a mixture of many different hydrocarbon compounds, most of which primarily contain carbon and hydrogen in varying proportions. Monocyclic aromatic hydrocarbons, which are small, explosive, and volatile, to large, non-volatile, are all included in the mixture of hydrocarbons. Toxicological effects of a hydrocarbon depend on its molecular weight, family of hydrocarbons, creature exposed to it, and life-cycle stage of the exposed organism. Studies have shown that exposure to the toxic chemicals in crude oil, such as para-phenol compounds and volatile benzene, might have an adverse effect on human health. Furthermore, when there is an oil spill at sea, the wind and water currents disperse the oil, causing a thin slick to migrate across the ocean’s surface. There are numerous simultaneous chemical and physical changes. Reviewing the long-term effects of petroleum on the environment is the aim of this paper in terms of the impact on human health, plants, and marine life.
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Abd Ghani, Shafinaz, Syahir Habib, Nur Adeela Yasid, Siti Aqlima Ahmad, and Mohd Yunus Shukor. "A Mini Review on the Biodegradation of Petroleum Hydrocarbon Pollutants by the Genus Rhodococcus." Journal of Environmental Microbiology and Toxicology 6, no. 2 (December 31, 2018): 1–6. http://dx.doi.org/10.54987/jemat.v6i2.436.

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The genus Rhodococcus is a very diverse group of Eubacteria that are commonly found in many environmental niches such as soils and seawaters likewise. Owed to their remarkable range of varied catabolic gene and robust cellular physiology, this genus is competent to degrade a great number of organic compounds including some of the most difficult compounds such as petroleum hydrocarbons. This review discusses two interrelated topics, which are the petroleum hydrocarbons pollution in the environment and the utilization of the Rhodococcus genus in microbial remediation of petroleum hydrocarbons and its derivatives. The first part focuses on the application of hydrocarbon components in standard routine, sources of pollution and the toxicity of these compounds. On the second fraction of this assessment, the biochemistry, physiology and genetic versatility of the genus Rhodococcus will be reviewed with emphasizing their significance in environmental biotechnology and bioremediation of petroleum hydrocarbons.
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17

AL-Kindi, A. Y. A., J. A. Brown, and C. P. Waring. "Endocrine, Physiological and Histopathological Responses of Fish and their Larvae to Stress with Emphasis on Exposure to Crude Oil and Various Petroleum Hydrocarbons." Sultan Qaboos University Journal for Science [SQUJS] 5 (December 1, 2000): 1. http://dx.doi.org/10.24200/squjs.vol5iss0pp1-30.

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Various endocrine and physiological responses of fish exposed to forceful physical and chemical stimuli are reviewed with emphasis on the effects of crude oils and their hydrocarbon constituents. The chemistry and toxicity of petroleum hydrocarbons are examined and methods for experimental exposure of fish to crude oil and petroleum hydrocarbons are considered. A variety of blood-borne parameters recognized as reliable tools in determining the relative severity of stress in fish are reviewed. The effects of stress and petroleum hydrocarbons on endocrine responses including changes in plasma catecholamines, corticosteroids, and thyroid hormones are reviewed. The physiological responses: changes in plasma glucose, osmotic and ionic regulation, blood oxygen, hematocrit and hemoglobin concentration are explored, and histopathological effects of crude oil on fish are reviewed. Recent studies of the effects of petroleum hydrocarbons on fish larvae are considered and the increased sensitivity of the early life stages of fish are highlighted.
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18

Thi Quynh Hoa, Kieu, Nguyen Vu Giang, Nguyen Thi Yen, Mai Duc Huynh, Nguyen Huu Dat, Vuong Thi Nga, Nguyen Thi Thu Ha, and Pham Thi Phuong. "Enhanced bioremediation of crude oil polluted water by a hydrocarbon-degrading Bacillus strain immobilized on polyurethane foam." Vietnam Journal of Biotechnology 18, no. 3 (November 28, 2020): 581–88. http://dx.doi.org/10.15625/1811-4989/18/3/15714.

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During the production and transportation of petroleum hydrocarbons, unsuitable operation and leakage may result in contamination of water and soil with petroleum hydrocarbons. Petroleum contamination causes significant marine environmental impacts and presents substantial hazards to human health. Bioremediation of contaminated water and soil is currently the effective and least harmful method of removing petroleum hydrocarbons from the environment. To improve the survival and retention of the bioremediation agents in the contaminated sites, microbial cells must be immobilized. It was demonstrated that immobilized microbial cells present advantages for degrading petroleum hydrocarbon pollutants compared to free suspended cells. In this study, the ability of a Bacillus strain (designed as Bacillus sp. VTVK15) to immobilize on PUF and to degrade crude oil was investigated. The immobilized Bacilllus strain had the highest number (5.38 ± 0.12 Í 108 CFU/g PUF) and a maximum attachment efficiency of 92% on PUF after 8 days. Analysis by GC-MS revealed that both free and immobilized cells of Bacillus sp. VTVK15 were able to degrade 65 and 90% of the hydrocarbons in 2% (v/v) crude oil tested after 14 days, respectively. The results suggest the potential of using PUF-immobilized Bacillus sp. VTVK15 to bioremediate petroleum hydrocarbons in an open marine environment.
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Nanekar, R. D., and S. S. Kokitkar. "Isolation, genetic identification and optimization of hydrocarbon degrading bacteria from petroleum contaminated soil in Raigad region." Ecology, Environment and Conservation 29, no. 04 (2023): 1554–61. http://dx.doi.org/10.53550/eec.2023.v29i04.014.

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In recent years, accumulation of petroleum hydrocarbons in soil and water is a global concern due to its genotoxicity and carcinogenic nature. Bioremediation of petroleum contaminates sites with natural isolates having ability to degrade hydrocarbons, are safe alternative for effective biodegradation. Present study is focused on isolation, characterization and optimization of novel isolates from petroleum contaminated soil with hydrocarbon degrading potential. Thirteen different petroleum contaminated soil sites were sampled from Raigad region to isolate indigenous hydrocarbon degrading bacteria. DCPIP screening assay was performed to select best isolates which utilize hydrocarbon as carbon source. The two best isolates S9D2 and S13D1 were identified as Rhodococcus ruber and Azospirillum zeae respectively by 16S rRNA gene-based sequencing and Phylogenetic analysis. One factor at a time (OFAT) with a spectrophotometer approach was used to optimize growth parameter. Optimum growth of Rhodococcus ruber was observed with 2 % diesel concentration at 37°C temperature and pH 7.0, while Azospirillum zeae showed better growth at 1% substrate concentration, pH 6.0. and temperature 30°C. The current study revealed that both the isolates have ability to degrade hydrocarbons present in diesel, and can be used for effective bioremediation tools
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Salari, Marjan, Vahid Rahmanian, Seyyed Alireza Hashemi, Wei-Hung Chiang, Chin Wei Lai, Seyyed Mojtaba Mousavi, and Ahmad Gholami. "Bioremediation Treatment of Polyaromatic Hydrocarbons for Environmental Sustainability." Water 14, no. 23 (December 6, 2022): 3980. http://dx.doi.org/10.3390/w14233980.

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Polycyclic aromatic hydrocarbons (PAHs) distributed in air and soil are harmful because of their carcinogenicity, mutagenicity, and teratogenicity. Biodegradation is an environmentally friendly and economical approach to control these types of contaminants and has become an essential method for remediating environments contaminated with petroleum hydrocarbons. The bacteria are isolated and identified using a mineral nutrient medium containing PAHs as the sole source of carbon and energy and biochemical differential tests. Thus, this study focuses on some bacteria and fungi that degrade oil and hydrocarbons. This study provides a comprehensive, up-to-date, and efficient overview of petroleum hydrocarbon contaminant bioremediation considering hydrocarbon modification by microorganisms, emphasizing the new knowledge gained in recent years. The study shows that petroleum hydrocarbon contaminants are acceptably biodegradable by some microorganisms, and their removal by this method is cost-effective. Moreover, microbial biodegradation of petroleum hydrocarbon contaminants utilizes the enzymatic catalytic activities of microorganisms and increases the degradation of pollutants several times compared to conventional methods. Biological treatment is carried out in two ways: microbial stimulation and microbial propagation. In the first method, the growth of indigenous microorganisms in the area increases, and the pollution is eliminated. In the second method, on the other hand, there are no effective microorganisms in the area, so these microorganisms are added to the environment.
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Nedi, Syahril, Irwan Effendi, Afrizal Tanjung, and Elizal Elizal. "Reduction of hydrocarbon pollutants by hyacinth plants (Eichhornia crassipes)." F1000Research 12 (June 22, 2023): 728. http://dx.doi.org/10.12688/f1000research.131846.1.

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Background: The application of phytoremediation by utilizing plants has been used to control oil pollution in waters. One of the plants that can act as a phytoremediator is the hyacinth because this plant can reduce various pollutants including petroleum hydrocarbons. This study aims to study the reduction ability of petroleum hydrocarbons at different concentrations including improving water quality. Methods: This study consisted of one treatment (petroleum hydrocarbon) consisting of five factors with three replicates. The treatments consisted of 10 ppm (E1), 30 ppm (E2), 50 ppm (E3), 70 ppm (E4), 90 ppm (E5), and (E0) without aquatic plants as controls. The treatments were observed daily and measured from the first day (D-1), the seventh day (D-7), and the 14th day (D-14). The water quality in each treatment was also measured, such as water temperature, pH, and dissolved oxygen. Results: The results showed that the hyacinth plant was able to reduce hydrocarbon in terms of total petroleum hydrocarbon (TPH) by 79% while it was only between 17–27% naturally without the hyacinth. The reduction of TPH in the water was in line with the decrease of chlorophyll in the leaves of hyacinths, and it was followed by the increase of dissolved oxygen in the water media. Conclusions: In conclusion, hyacinths can reduce petroleum hydrocarbons and they can improve the water quality as well.
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Yong, Raymond N., and Sudhakar M. Rao. "Mechanistic evaluation of mitigation of petroleum hydrocarbon contamination by soil medium." Canadian Geotechnical Journal 28, no. 1 (February 1, 1991): 84–91. http://dx.doi.org/10.1139/t91-009.

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Present in situ chemical treatment technologies for mitigation of petroleum hydrocarbon contamination are in the developmental stage or being tested. To devise efficient strategies for restricting the movement of petroleum hydrocarbon (PHC) molecules in the contaminated soil, it is proposed to utilize the sorption–interaction relationships between the petroleum contaminants and the soil substrate. The basic questions addressed in this paper are as follows (i) What are the prominent chemical constituents of the various petroleum fractions that interact with the soil substrate? (ii) What are the functional groups of a soil that interact with the contaminants? (iii) What are the bonding mechanisms possible between the soil functional groups and the PHC contaminants? (iv) What are the consequent changes brought about the soil physical properties on interaction with PHC's? (v) What are the factors influencing the interactions between PHC molecules and clay particles of the soil substrate? (vi) What is the possibility of improving the soil's attenuation ability for PHC's? The development of answers to the basic questions reveal that petroleum hydrocarbons comprise a mixture of nonpolar alkanes and aromatic and polycyclic hydrocarbons, that have limited solubility in water. The bonding mechanism between the nonpolar PHC's and the clay surface is by way of van der Waals attraction. The adsorption of the nonpolar hydrocarbons by the clay surface occurs only when their (i.e., the hydrocarbon molecules) solubility in water is exceeded and the hydrocarbons exist in the micellar form. Dilute solutions of hydrocarbons in water, i.e., concentrations of hydrocarbons at or below the solubility limit, have no effect on the hydraulic conductivity of clay soils. Permeation with pure hydrocarbons invariably influences the clay hydraulic conductivity. To improve the attenuation ability of soils towards PHC's, it is proposed to coat the soil surface with "ultra" heavy organic polymers. Adsorption of organic polymers by the clay surface may change the surface properties of the soil from highly hydrophilic (having affinity for water molecules) to organophilic (having affinity for organic molecules). The organic polymers attached to the clay surface are expected to attenuate the PHC molecules by van der Waals attraction, by hydrogen bonding, and also by adsorption into interlayer space in the case of soils containing swelling clays. Key words: petroleum hydrocarbons (PHC's), bonding mechanisms, functional groups, PHC-soil interaction, permeation, hydraulic conductivity, attenuation, van der Waals, organic polymers, organophilic.
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Barnes, David L., Sarah M. Wolfe, and Dennis M. Filler. "Equilibrium distribution of petroleum hydrocarbons in freezing ground." Polar Record 40, no. 3 (July 2004): 245–51. http://dx.doi.org/10.1017/s0032247404003602.

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Past documented laboratory measurements have shown movement of petroleum hydrocarbons to the freezing front in contaminated freezing soils. The mechanisms that are, in part, responsible for the increased contaminant concentration at the freezing front are illustrated in this study with a mass-balance model. Results from this quantitative analysis show that this concentration increase is due to exclusion of petroleum hydrocarbon from the crystalline ice structure and from physical displacement of liquid petroleum hydrocarbon from the pore space as water freezes and expands into ice. Consequences of this process in relation to contaminant migration in freezing soils through time are discussed.
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Al-Mebayedh, Hamad, Anyi Niu, and Chuxia Lin. "Petroleum Hydrocarbon Composition of Oily Sludge and Contaminated Soils in a Decommissioned Oilfield Waste Pit under Desert Conditions." Applied Sciences 12, no. 3 (January 27, 2022): 1355. http://dx.doi.org/10.3390/app12031355.

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Field and laboratory investigations were conducted to characterize the composition of petroleum hydrocarbons in oily sludge and the underlying contaminated soils in a decommissioned oilfield waste pit in Kuwait. The results show that the petroleum hydrocarbon composition in the oily sludge and contaminated soils was spatially variable. Highly toxic petroleum hydrocarbon species such as BTEX and PAHs were generally lacking, and both sludge- and soil-borne hydrocarbons were dominated by long-chain petroleum hydrocarbons. The soil contamination depth was generally very shallow although localized deep profiles (>0.5 m) were found. A loose relationship was established between TPH in the sludge and that in the underlying soil. On average, the soil had a greater percentage of shorter-chain hydrocarbon fractions (either aliphatics or aromatics), as compared to the sludge. The environmental risk from the oily sludge and contaminated soils is considered to be relatively low. For cost-effective management of the environmental risk of decommissioning an oilfield waste pit, containment of the sludge and contaminated soils using a soil-capping approach may be sufficient to minimize the possible adverse environmental impacts from the decommissioned waste pit, and this may represent an option that is superior to other costly remediation strategies.
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25

Ikpe, E. E., U. U. Ubong, E. Uwanta, U. U. Oku, and E. O. Akpan. "Investigation of Petroleum Hydrocarbons in Soil from Auto-mobile Mechanic Workshops within Ikot Akpaden, Mkpat Enin L.G.A Akwa Ibom State Nigeria." Chemical Science International Journal 32, no. 2 (March 10, 2023): 22–29. http://dx.doi.org/10.9734/csji/2023/v32i2842.

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This study investigates the concentrations of total petroleum hydrocarbon (TPH) and total hydrocarbon content (THC) in soil samples from auto-mobile mechanic workshop in Ikot Akpaden, Mkpat Enin. Soil samples were collected in triplicate at different points within the vicinity of auto-mobile mechanic workshop at Ikot Akpaden represented as site A and control samples were obtained within the frontage of Chemistry Department, AKSU represented as site C. Soxhlet extraction and shaking methods were used for the extraction of the total petroleum hydrocarbons and total hydrocarbon content respectively. Each of the sample extracts were purified using column chromatography to remove moisture, polar hydrocarbons, colour interferences and any impurities before GC analysis. Total petroleum hydrocarbons and THC were analyzed using Gas Chromatography (GC-FID) and UV-VISIBLE spectrophotometer respectively. The results obtained from TPH analysis indicates that; 15,223.4 mg/kg was recorded from the auto-mobile mechanic workshop and 808.507 mg/kg was recorded from the control samples. The results from the two stations were higher than the department of petroleum resources (DPR) permissible limit of 50 mg/Kg for soils. There was significant difference in the concentration of total hydrocarbon content, the result obtained showed that, the three sampling point at the mechanic workshop has THC level in the order; A1>A3>A2. The concentration of THC obtained in the three points were greater than the 0.6 mg/Kg limit of total hydrocarbons content allowed by the DPR. The study revealed that the soils were polluted as a result of indiscriminate disposal of waste petroleum products (used engine oil, petrol, diesel and other lubricants) directly on the soil, as well as dumping and burning of waste at the auto-mobile mechanic workshops. Hence, there is need for a holistic and sustainable monitoring and remediation of the environment for a cleaner and healthier environment.
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26

Gofar, Nuni. "Characterization of Petroleum Hydrocarbon Decomposing Fungi Isolated from Mangrove Rhizosphere." Journal of Tropical Soils 16, no. 1 (July 1, 2013): 39–45. http://dx.doi.org/10.5400/jts.2011.v16i1.39-45.

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The research was done to obtain the isolates of soil borne fungi isolated from mangrove rhizosphere which were capable of degrading petroleum hydrocarbon compounds. The soil samples were collected from South Sumatra mangrove forest which was contaminated by petroleum. The isolates obtained were selected based on their ability to survive, to grow and to degrade polycyclic aromatic hydrocarbons in medium containing petroleum residue. There were 3 isolates of soil borne hydrocarbonoclastic fungi which were able to degrade petroleum in vitro. The 3 isolates were identified as Aspergillus fumigates, A. parasiticus, and Chrysonilia sitophila. C. sitophila was the best isolate to decrease total petroleum hydrocarbon (TPH) from medium containing 5-20% petroleum residue.Keywords: Hydrocarbonoclastic fungi, hydrocarbon compounds, mangrove rhizosphere
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27

Wang, Zhendi, and Mervin F. Fingas. "Identification of the Source(s) of Unknown Spilled Oils." International Oil Spill Conference Proceedings 1999, no. 1 (March 1, 1999): 211–18. http://dx.doi.org/10.7901/2169-3358-1999-1-211.

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ABSTRACT The ability to unambiguously identify spilled oils and petroleum products in complex contaminated environmental samples and to link them to the known sources is extremely important in settling questions of environmental impacts and liability. This paper will briefly review advanced chemical fingerprinting and data interpreting techniques used to identify sources of spilled oils. The chemical fingerprinting techniques discussed include pattern recognition evaluation of target petroleum hydrocarbon distributions; determination of major oil components and hydrocarbon groups; determination of diagnostic ratios of source-specific marker compounds such as PAHs and biomarkers; target PAH isomer analysis; and carbon isotopic ratio analysis. Methods for distinguishing biogenic and pyrogenic hydrocarbons from petrogenic hydrocarbons are also addressed. Several examples are presented to illustrate approaches to identifying and allocating multiple sources of hydrocarbons in complex hydrocarbon mixtures using these advanced chemical fingerprinting techniques.
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28

Parnell, John, and Paul Eakin. "The replacement of sandstones by uraniferous hydrocarbons: significance for petroleum migration." Mineralogical Magazine 51, no. 362 (October 1987): 505–15. http://dx.doi.org/10.1180/minmag.1987.051.362.05.

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AbstractHydrocarbons (bitumens sensu lato) in sandstones have been recorded in several instances to have partially replaced their host rock, including quartz grains. Many replacive hydrocarbons are uranium-rich: associated non-uraniferous hydrocarbons are not replacive. Uranium is transported as carbonate complexes, which may be decomposed by organic acids to yield UO22+ ions and CO2. The UO22+ will be absorbed onto hydrocarbons before reduction to a mineral phase, generally uraninite; and the CO2 may be aggressive towards the silicate grains of the host sandstone. Accretionary nodules of replacive uraniferous hydrocarbon in red beds (e.g. at the cores of reduction spots) can provide valuable information about petroleum migration. They occur particularly in the vicinity of faults, and may record the interactions between metal-rich groundwaters and hydrocarbons leaking along a fault from an underlying reservoir. A trial study in Devonian sandstones of Easter Ross successfully traced a hydrocarbon-bearing sandstone reservoir from an occurrence of uraniferous hydrocarbon nodules.
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29

Petrov, Al A., and N. N. Abryutina. "Isoprenoid petroleum hydrocarbons." Russian Chemical Reviews 58, no. 6 (June 30, 1989): 575–87. http://dx.doi.org/10.1070/rc1989v058n06abeh003461.

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30

Ismayilova, M. K., I. I. Mustafayev, S. Z. Melikova, F. N. Nurmammadova, and M. H. Aliyeva. "Radiation-induced isomerization reaction mechanism of hydrocarbons on the surface of solid acid." Physics and Chemistry of Solid State 24, no. 3 (September 13, 2023): 460–66. http://dx.doi.org/10.15330/pcss.24.3.460-466.

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This paper's goal is to investigate how petroleum's n-alkane isomerization reaction occurs on the surface of a solid nanocatalyst. In this experiment, the isomerization of aromatic-rich crude oil is being studied as a result of radiation exposure. It was discovered that sodium-rich bentonite –solid acid, from the Alpoid deposit works well as a catalyst for refining petroleum. Possible mechanisms of the radio-catalytic reaction that converts n-alkanes to branched hydrocarbons have been researched by methods of FT- IR spectroscopy and gas chromatography. The effect of acidity type of the nanostructured bentonite clay on Lewis acid cites is proposed to explain observed results. Additional radiation energy, which is mainly generated due to the nanobentonite volume, results in a more efficient aromatic- rich petroleum radiolysis process. Also, it was investigated the dynamics of dose –dependent changes in the amount of branched hydrocarbons. One of the primary objectives of this research work is increasing the efficiency of transformation of adsorbed beam energy in solid phase to the system and improvement of hydrocarbon isomerization reactions in crude oil. The results of hydrocarbon transformations are discussed in terms of the behavior of excited electrons in solids. Novel eco-friendly nanocatalyst creates new opportunities for hydrocarbons with multi- branched structures.
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31

Hegazy, Ahmad K., Zahra S. Hussein, Nermen H. Mohamed, Gehan Safwat, Mohamed A. El-Dessouky, Ilinca Imbrea, and Florin Imbrea. "Assessment of Vinca rosea (Apocynaceae) Potentiality for Remediation of Crude Petroleum Oil Pollution of Soil." Sustainability 15, no. 14 (July 14, 2023): 11046. http://dx.doi.org/10.3390/su151411046.

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Petroleum oil pollution is a worldwide problem that results from the continuous exploration, production, and consumption of oil and its products. Petroleum hydrocarbons are produced as a result of natural or anthropogenic practices, and their common source is anthropogenic activities, which impose adverse effects on the ecosystem’s nonliving and living components including humans. Phytoremediation of petroleum hydrocarbon-polluted soils is an evolving, low-cost, and effective alternative technology to most traditional remediation methods. The objective of this study is to evaluate the phytoremediation potentiality of Vinca rosea for crude oil-contaminated soil by understanding its properties and involvement in the enhanced degradation of crude oil. The remediation potentiality was determined by evaluating the total petroleum hydrocarbon degradation percentage (TPH%) and changes in the molecular type composition of saturated and aromatic hydrocarbon fractions. TPH% was estimated gravimetrically, and changes in the molecular type composition of saturated and aromatic fractions were measured using gas chromatography and high-performance liquid chromatography, respectively. Sulfur concentration was measured using X-ray fluorescence. Cadmium and lead quantification was measured using Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES). The results revealed that V. rosea enhanced total petroleum hydrocarbon (TPH) degradation and altered the molecular composition of the crude oil. The saturated hydrocarbons increased and the aromatic hydrocarbons decreased. The saturated hydrocarbon fraction in the crude oil showed a wider spectrum of n-paraffin peaks than the oil extracted from unplanted and V. rosea-planted soils. Polyaromatic hydrocarbon degradation was enhanced in the presence of V. rosea, which was reflected in the increase of monoaromatic and diaromatic constituents. This was parallel to the increased sulfur levels in planted soil. The determination of sulfur and heavy metal content in plant organs indicated that V. rosea can extract and accumulate high amounts from polluted soils. The ability of V. rosea to degrade TPH and alter the composition of crude petroleum oil by decreasing the toxicity of polyaromatic hydrocarbons in soil, as well as its capability to absorb and accumulate sulfur and heavy metals, supports the use of plant species for the phytoremediation of crude oil-polluted sites.
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32

Simoneit, Bernd R. T. "Hydrothermal petroleum: genesis, migration, and deposition in Guaymas Basin, Gulf of California." Canadian Journal of Earth Sciences 22, no. 12 (December 1, 1985): 1919–29. http://dx.doi.org/10.1139/e85-208.

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Hydrothermal activity and seabed mounds have been explored in Guaymas Basin by the Deep Sea Drilling Project (DSDP), piston coring, dredging, and diving with the Deep Submersible Research Vessel (DSRV) Alvin. Sedimentary organic matter, derived primarily from immature, degraded microbial detritus, is easily converted to petroleum under the hydrothermal regime. These petroleums are mature and migrate in the fluids and by diffusion to the seabed. The fluid migration is aided by near-critical aqueous solution and supercritical carbon dioxide and hydrocarbons. Petroleum compositions vary from condensates to naphthenic to waxy, all with significant amounts of asphaltenes and hydrothermal products such as olefins and toxic polynuclear aromatic hydrocarbons (PAH). The heavy ends condense at the seabed, depositing mainly as a cement with the sulfides and other minerals and to a lesser extent as entrapped oil and crystalline wax in vugs and conduits of the mounds. The PAH are high-temperature resynthesis-aromatization products from residual organic matter, and they are present in all oils but also deposit as discrete trace fractions in the hottest regions of the vent systems. Preliminary estimates of total hydrocarbon generation during hydrothermal alteration indicate that this process has a significant petroleum potential.
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33

Habib, Syahir, Wan Lutfi Wan Johari, Mohd Yunus Shukor, and Nur Adeela Yasid. "Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP." Bioremediation Science and Technology Research 5, no. 2 (December 31, 2017): 13–16. http://dx.doi.org/10.54987/bstr.v5i2.358.

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Petroleum hydrocarbons remain as the major contaminants that could be found across the world. Remediation approach through the utilisation of microbes as the bioremediation means is widely recognised due to their outstanding values. As a result, scientific reports on the isolation and identification of new hydrocarbon-degrading strains were on the rise. Colourimetric-based assays are one of the fastest methods to identify the capability of hydrocarbon-degrading strains in both qualitative and quantitative assessment. In this study, the hydrocarbon-degrading potential of nine bacterial isolates was observed via 2,6-dichlorophenolindophenol (DCPIP) test. Two potent diesel-utilising isolates show a distinctive tendency to utilise aromatic (ADL15) and aliphatic (ADL36) hydrocarbons. Both isolates prove to be a good candidate for bioremediation of wide range of petroleum hydrocarbon components.
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34

Nemirovskaya, I. A., V. D. Oneginа, A. P. Lisitzin, and B. V. Konovalov. "Origin of hydrocarbons in suspended matter and bottom sediments near the Crimean peninsula." Доклады Академии наук 484, no. 5 (May 16, 2019): 600–604. http://dx.doi.org/10.31857/s0869-56524845600-604.

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It was established that the content of hydrocarbons in the surface waters of the Feodosia Gulf fluctuated were varied a wide range (11–179 μg/l), and exceeded the MPC values for petroleum hydrocarbons in individual samples. The highest concentrations were observed in 2016. The composition of alkanes suggested their mixed (autochthonous and allochthonous) origin. The weathered petroleum hydrocarbons have been identified only in a few cases. In bottom sediments, the concentrations of hydrocarbons depended on their grain size distribution, and terrigenous alkanes were dominated in their composition. The oil and pyrogenic hydrocarbons’ pollution were established based on the composition of polycyclic aromatic hydrocarbons. In the open part of the Black Sea, southward the Crimean peninsula, the hydrocarbon content in surface waters and bottom sediments were at the level of background concentrations, and homologs of terrigenous origin dominated in their composition.
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35

Bojesen-Koefoed, Jørgen A., and H. Peter Nytoft. "Petroleum geochemistry of the deepened Lopra-1/1A re-entry well, Faroe Islands." Geological Survey of Denmark and Greenland (GEUS) Bulletin 9 (May 31, 2006): 67–77. http://dx.doi.org/10.34194/geusb.v9.4860.

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The Lopra-1/1A re-entry well was drilled as a stratigraphic test with no immediate exploration objectives. Hence, petroleum geochemical studies were of limited extent, and restricted to non-destructive analyses. The presence of natural petroleum hydrocarbons could not be confirmed with certainty, but hydrocarbons extracted from the hydrochloric acid solute of a calcite vug present in RSWC #1 (3543 m), may represent indigenous petroleum since hydrocarbon-bearing fluid inclusions have been reported from the same sample. These hydrocarbons show some similarities to petroleum generated from the Upper Jurassic – Lower Cretaceous Kimmeridge Clay type source rocks present in surrounding areas. Except for this sample, the results generally show the presence of a variety of contaminants of different origins such as ‘naturally greasy fingers’ (squalene and cholesterol), cosmetics such as chap stick or hand lotion (e.g. esters such as butyl-stearate, stearyl-palmitate, vitamin A), plasticisers (phthalates), diesel oil and ‘pipe dope’.
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36

Shapiro, T. N., N. A. Manucharova, and E. S. Lobakova. "Activity of alkanmonooxygenase <i>alk</i>B gene in strains of hydrocarbon-oxidizing bacteria isolated from petroleum products." Vavilov Journal of Genetics and Breeding 26, no. 6 (October 9, 2022): 575–82. http://dx.doi.org/10.18699/vjgb-22-70.

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Alkanmonooxygenase enzymes AlkB and Cyp153 are responsible for the aerobic degradation of n-alkanes of petroleum and petroleum products. To prove the usage of n-alkanes from oil and petroleum products by hydrocarbon- oxidizing bacteria isolated from aviation kerosene TS-1 and automobile gasoline AI-95, the detection of the key genes alkB, Alk1, Alk2, Alk3 and Cyp153 encoding alkanmonooxygenases AlkB and Cyp153 (responsible for the oxidation of hydrocarbons with a certain chain length) was carried out. It was found that bacterial strains isolated from TS-1 jet fuel, except Deinococcus sp. Bi7, had at least one of the studied n-alkane degradation genes. The strains Sphingobacterium multivorum Bi2; Alcaligenes faecalis Bi3; Rhodococcus sp. Bi4; Sphingobacterium sp. Bi5; Rhodococcus erythropolis Bi6 contained the alkB gene. In the strains of hydrocarbon-oxidizing bacteria isolated from gasoline AI- 95, this alkanmonooxygenase gene was not detected. Using the real-time PCR method, the activity of the alkB gene in all bacterial strains isolated from petroleum products was analyzed and the number of its copies was determined. By real-time PCR using a primer with a different sequence of nucleotides to detect the alkB gene, its activity was established in all bacterial strains isolated from gasoline AI-95; besides, the strain Paenibacillus agaridevorans Bi11 was assigned to the group with a high level of its activity (1290 copies/ml). According to the assessment of the growth of isolated hydrocarbon-oxidizing bacteria on a solid Evans mineral medium with the addition of the model mixture of hydrocarbons, the strains were divided into three groups. The distributions of strains of hydrocarbon-oxidizing bacteria in the groups based on the activity of the alkB gene and groups formed based on the growth ability and use of the model mixture of hydrocarbons and petroleum products were found to be consistent. The results obtained indicate that we need to use a complex of molecular and physiological methods for a comprehensive analysis of the distribution of the studied genes in bacteria and to assess their activity in the strains of hydrocarbon-oxidizing bacteria capable of biodegradation of petroleum hydrocarbons.
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37

Jiménez, Oscar Pindado, Rosa M. Pérez Pastor, and Olga Escolano Segovia. "An analytical method for quantifying petroleum hydrocarbon fractions in soils, and its associated uncertainties." Anal. Methods 6, no. 15 (2014): 5527–36. http://dx.doi.org/10.1039/c4ay00097h.

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38

Etukokwu, Ugochukwu Alex, Godwin Jeremiah Udom, and Ferdinand Giadom. "Lagena Inferocostata – An Opportunistic Index Species of Hydrocarbon Pollutants in Gokana and Khana Local Government Areas of River State, Nigeria." International Journal of Research and Innovation in Social Science VII, no. IV (2023): 779–90. http://dx.doi.org/10.47772/ijriss.2023.7464.

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Thirty shallow cores ranging from 0.0 – 1.0m and composited into upper and lower section, was studied to determine relationship between petroleum hydrocarbon pollutants and benthic foraminiferal species in order to determine an index for environmental monitoring in the study area. The core samples were taken from Gokana and Khana Local Government Areas of Rivers State, Nigeria. The cores were treated with Rose Bengal solution to distinguish between then acoenose and biocoenosis benthonic foraminiferal. The cores were analyzed for sedimentology, petroleum hydrocarbon concentrations and micropaleontology. Sedimentological descriptions of the cores indicated inhibited free flow of fluids based on the rock textures which mostly consists of clays and peaty clays. Petroleum hydrocarbons analysis consisted of Total Petroleum Hydrocarbon (TPH) and Polycyclic Aromatic Hydrocarbon (PAH). The results in concentrations of the petroleum hydrocarbons indicated higher concentrations in the upper composited sections. Total Petroleum Hydrocarbon (TPH) and Polycyclic Aromatic Hydrocarbon (PAH) ranged between (20612mg/kg – 37.09mg/kg and 9.55mg/kg – <0.01mg/kg respectively) in the upper composited section and between (14731mg/kg – 14.67mg/kg and 5.71mg/kg – <0.01mg/kg respectively) in the lower composited section. Occurrence of benthonic foraminiferal in the study samples is very low to barren among very low diversity. Three benthonic foraminiferal diversities were identified in the study samples and included Lagenainferocostata, Anomalinoidesspp and Ammodiscus spp. Benthonic foraminifera showed negative correlation with TPH/PAH in the presence of carcinogenic Polycyclic Aromatic Hydrocarbon (PAH) congeners except for Lagenainferocostata which increased consistently and significantly with increasing TPH/PAH in the two composited sections. This suggested Lagenainferocostata as an Index opportunistic species for the hydrocarbon polluted study area.
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39

Agrawal, Ishita. "Oil Degrading Bacteria: Remediation of Environmental Pollution Resulting from Petroleum Hydrocarbons." Biotechnology Kiosk 2, no. 10 (October 5, 2020): 5–10. http://dx.doi.org/10.37756/bk.20.2.10.1.

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It is widely known that petroleum hydrocarbons constitute one of the most hazardous pollutants that affect human and environmental health. The ongoing research on bioremediation with petroleum hydrocarbon-degrading bacteria has shown tremendous promise of the technology due to its advantages of high efficiency and eco-friendly nature. To this end, studies have been carried out to identify a large amount of bacterial species with petroleum hydrocarbon-degrading ability for applications in bioremediation. Here, we present a brief perspective of some of the notable advances in oil degrading bacteria and the remedial actions for decontamination of water and soil along with recovering the spilled materials at oil sites.
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40

Iorga, Cristian Mugurel, Maria Cătălina Ţopa, and Mihaela Marilena Stancu. "The study on the possibility of using sewage sludge in the ecological reconstruction of contaminated sites from the petroleum industry." Annals of the ”Dunarea de Jos” University of Galati Fascicle II Mathematics Physics Theoretical Mechanics 46, no. 1 (September 11, 2023): 24–31. http://dx.doi.org/10.35219/ann-ugal-math-phys-mec.2023.1.05.

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One field of activity that pollutes important land surfaces with hydrocarbons is the petroleum industry. In the ecological reconstruction of contaminated sites from the petroleum industry, natural geological resources are used to fill the excavations resulting from the decommissioning of some equipment and installations. Increasing amounts of sewage sludge are generated by the growing number of municipal wastewater treatment plants. For this reason, solutions are being sought for the elimination or utilization of sewage sludge. For the decontamination of soils polluted with petroleum hydrocarbons, bioremediation technologies with the help of microorganisms, especially bacteria are used on a large scale. From the results carried out in the present study, it appears that sewage sludge has microbiological, agrochemical, and geotechnical characteristics, which could make possible their utilization for the ecological reconstruction of sites contaminated with petroleum hydrocarbon. Thus, it would be possible to capitalize on the sewage sludge in the petroleum industry.
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41

Mostafa, Aya A., Ahmad K. Hegazy, Nermen H. Mohamed, Rehab M. Hafez, Ehab Azab, Adil A. Gobouri, Hosam A. Saad, Azza M. Abd-El Fattah, and Yasser M. Mustafa. "Potentiality of Azolla pinnata R. Br. for Phytoremediation of Polluted Freshwater with Crude Petroleum Oil." Separations 8, no. 4 (March 26, 2021): 39. http://dx.doi.org/10.3390/separations8040039.

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The pollution of freshwater resources with crude petroleum oil is a major environmental issue in oil-producing countries. As a result, the remediation of polluted aquatic ecosystems using eco-friendly and cost-effective technology is receiving increased global attention. In this study, the ability of Azolla pinnata R. Br. to remediate petroleum-polluted freshwater was assessed. The remediation potentiality was determined by evaluating the total petroleum hydrocarbon degradation percentage (TPH%) and changes in the molecular type composition of saturated and aromatic hydrocarbon fractions. TPH% was estimated gravimetrically, and changes in the molecular type composition of saturated and aromatic fractions were measured using gas chromatography and high-performance liquid chromatography, respectively. The results reveal that A. pinnata has the potential to phytoremediate freshwater polluted with low levels (up to 0.5 g/L) of petroleum hydrocarbons (PHs). After seven days of phytoremediation, the degradation rate of total PHs was 92% in the planted treatment compared with 38% in the unplanted positive control. The highest breakdown of PHs for the normal paraffinic saturated hydrocarbon fraction occurred in the presence of A. pinnata combined with Anabena azollaea (A-A), which showed a moderate degradation capacity toward total aromatic hydrocarbons (TAHs) and total polycyclic aromatic hydrocarbons (PAHs). The results indicate that A. pinnata effectively removed C18, a saturated PH, and acenaphthene (Ace), an aromatic PH. Therefore, this study suggests that A. pinnata is a useful tool for the remediation of freshwaters contaminated with low pollution levels of crude oil.
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42

Zhang, Z. Z., S. M. Su, Y. J. Luo, and M. Lu. "Improvement of natural microbial remediation of petroleum-polluted soil using graminaceous plants." Water Science and Technology 59, no. 5 (March 1, 2009): 1025–35. http://dx.doi.org/10.2166/wst.2009.081.

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A 150-day pot experiment was conducted with graminaceous plants grown in natural soil contaminated with petroleum. The relationships among microbial activity, dehydrogenase activity, catalase activity, soil moisture, and the petroleum degradation rate were analyzed. All three plants accelerated the degradation of petroleum compared with unplanted soil. Plant roots improved the soil moisture by about 5% (from 15% in unplanted soil to 20% in soil containing plant roots), and the number of microorganisms in the rhizosphere increased by more than three orders of magnitude. The induction of the rhizosphere environment and the intimidation of the petroleum changed the abundance and activity of the microorganisms. Dehydrogenase activity in the rhizosphere was 1.54 to 1.87 times the value in the unplanted soil, but catalase activity was 0.90 to 0.93 times the value in unplanted soil. The petroleum degradation rates in the rhizosphere were 2.33 to 3.19 times higher than in the unplanted soil. The effect of rhizosphere degradation clearly changed the hydrocarbon composition, increasing the degradation of alkane hydrocarbons with low and moderate carbon contents. The rhizosphere environment promoted degradation of the high-carbon-content hydrocarbons into low-carbon-content hydrocarbons. At the same time, the Pr/nC17, Ph/nC18, and Pr/Ph values increased by 0.99 and 2.69 units, and decreased by 1.25 units, respectively, compared with the undegraded oil. The plants also accelerated the isomerization of alkane hydrocarbons.
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43

Guo, Weijun, Xinggang Wang, Sihong Liu, Xiangpeng Kong, Pengcheng Wang, and Tiaojian Xu. "Long-Term Petroleum Hydrocarbons Pollution after a Coastal Oil Spill." Journal of Marine Science and Engineering 10, no. 10 (September 27, 2022): 1380. http://dx.doi.org/10.3390/jmse10101380.

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The long-term status of petroleum hydrocarbons in both seawater and sediment contaminated by the Dalian New Port oil spill has been investigated since 2010. Seawater recovery is relatively swift and is complete within two years, while oil contamination persists in the coastal sediments for several years. Because of the slow degradation and low mobility in sediments, they serve as long-term reservoirs for residual oils. The erosion of sediments into the water column leads to an abrupt increase in hydrocarbons during storms. The cumulative results of hydrodynamic transport and ongoing industrial emissions lead to a spatial shift of hot spots with high petroleum hydrocarbon concentrations from the spill site to the inner corner of the bay. In addition to continuous petroleum hydrocarbon emissions from contiguous coastal outfalls, the regional oil contamination will persist indefinitely. The research provides comprehensive information for years to come to evaluate the long-term damage and multiphase medium impacts of a large oil spill.
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44

Chelomin, Victor Pavlovich, Valentina Vladimirovna Slobodskova, Sergey Petrovich Kukla, Elena Vladimirovna Zhuravel, and Andrey Pavlovich Chernyaev. "Genotoxic Effects of Exposure to Water-Soluble Fraction of Diesel Fuel in Sand Dollar Scaphechinus mirabilis Gametes." Toxics 11, no. 1 (December 28, 2022): 29. http://dx.doi.org/10.3390/toxics11010029.

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Pollution of marine areas with oil and oil products is steadily growing. As part of this connection, the study of the impact of petroleum hydrocarbons on marine hydrobionts is an urgent issue of modern ecotoxicology. In our study, the genotoxic effect of the water-soluble fraction of diesel fuel at different concentrations on the gametes of the sand dollar Scaphechinus mirabilis was evaluated. It was shown that during the incubation of sperm and eggs of a sand dollar in WAF with an oil hydrocarbon content of 1.32; 2.64; 5.37; 7.92 mg/L caused the destruction of the DNA molecule to varying degrees in both types of gametes. In addition, it has been shown that with an increase in the concentration of petroleum hydrocarbons in WAF, a large number of cells with a high level of DNA damage appear. The success of fertilization after exposure of gametes to a water-soluble extract of petroleum hydrocarbons was also evaluated. The relationship between an increase in the concentration of hydrocarbons in the tested solutions and a decrease in the level of fertilization is shown.
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45

Okoli, Ejike, Ify L. Nwaogazie, and Ejikeme Ugwoha. "Seasonal Variation of Groundwater Quality in Bonny Island, Rivers State Nigeria." International Journal of Environment and Climate Change 14, no. 5 (May 10, 2024): 105–14. http://dx.doi.org/10.9734/ijecc/2024/v14i54174.

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This study investigated the seasonal variations in physicochemical parameters, heavy metals, and petroleum hydrocarbons in groundwater samples collected in nine locations in Bonny Island, Rivers State Nigeria. The water samples for the dry season were collected in December and January while for the wet season, water samples were collected in July and October. A quantitative approach was employed, involving the collection of quantitative data through field sampling and laboratory analysis. Physicochemical parameters, including pH, electrical conductivity, dissolved oxygen, and nutrient levels, were measured using standard analytical techniques. Heavy metal concentrations (Fe, Cd, etc.) were determined using atomic absorption spectrophotometry, while petroleum hydrocarbons (total hydrocarbons and polycyclic aromatic hydrocarbons) were analyzed through solvent extraction and gas chromatography techniques. The study revealed significant seasonal variations in several parameters, with notable significant decreases in phosphate during the wet season and significant increases in chromium and cadmium levels during the dry season (p-value < 0.05). Petroleum hydrocarbon concentrations also exhibited seasonal fluctuations, potentially influenced by precipitation patterns, oil and gas activities, and accidental spills or leaks which were observed during the dry season.
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46

Wang, Xin Xin, Chen Li, Li Bin Zhao, Liang Wu, Wei An, and Yu Chen. "Diversity of Culturable Hydrocarbons-Degrading Bacteria in Petroleum-Contaminated Saltern." Advanced Materials Research 1010-1012 (August 2014): 29–32. http://dx.doi.org/10.4028/www.scientific.net/amr.1010-1012.29.

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Microbial degradation of hydrocarbon in contaminated salterns has attracted increasing attention. However, the diversity of hydrocarbons-degrading bacteria in such environments was still poorly understood. A total of 14 bacteria were isolated from a petroleum-contaminated saltern, which could grow in 3% NaCl. Especially, 2 isolates can survive in 20% NaCl. In addition, all isolates degraded petroleum. However, only 12, 8 and 3 isolates degraded phenanthrene, pyrene and n-Hexadecane, respectively. Phylogenetic analysis showed the isolates belonged to the generaAchromobacter,Rhodococcus,Mycobacteriumi,Dietzia,Sphingobium,Pseudomonas,Ochrobactrum,Bacillu,Rhizobium,Halomonas,Idiomarina,ChromohalobacterandMarinobacter. Hydrocarbon-degrading activity ofAchromobacter pulmonis,Bacillus persicus,Rhizobium helanshanense,Halomonas xianhensisandIdiomarina loihiensisin petroleum-contaminated saltern was reported for the first time.
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47

Castro, Ana R., Gilberto Martins, Andreia F. Salvador, and Ana J. Cavaleiro. "Iron Compounds in Anaerobic Degradation of Petroleum Hydrocarbons: A Review." Microorganisms 10, no. 11 (October 29, 2022): 2142. http://dx.doi.org/10.3390/microorganisms10112142.

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Waste and wastewater containing hydrocarbons are produced worldwide by various oil-based industries, whose activities also contribute to the occurrence of oil spills throughout the globe, causing severe environmental contamination. Anaerobic microorganisms with the ability to biodegrade petroleum hydrocarbons are important in the treatment of contaminated matrices, both in situ in deep subsurfaces, or ex situ in bioreactors. In the latter, part of the energetic value of these compounds can be recovered in the form of biogas. Anaerobic degradation of petroleum hydrocarbons can be improved by various iron compounds, but different iron species exert distinct effects. For example, Fe(III) can be used as an electron acceptor in microbial hydrocarbon degradation, zero-valent iron can donate electrons for enhanced methanogenesis, and conductive iron oxides may facilitate electron transfers in methanogenic processes. Iron compounds can also act as hydrocarbon adsorbents, or be involved in secondary abiotic reactions, overall promoting hydrocarbon biodegradation. These multiple roles of iron are comprehensively reviewed in this paper and linked to key functional microorganisms involved in these processes, to the underlying mechanisms, and to the main influential factors. Recent research progress, future perspectives, and remaining challenges on the application of iron-assisted anaerobic hydrocarbon degradation are highlighted.
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48

UZAIR, B., M. MUNIR, S. TASSADAQ, S. KHAN, and B. A. KHAN. "BACTERIA-MEDIATED DEGRADATION OF PETROLEUM HYDROCARBON CONTAMINANTS: AN OVERVIEW." Latin American Applied Research - An international journal 46, no. 4 (October 31, 2016): 139–46. http://dx.doi.org/10.52292/j.laar.2016.345.

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One of the major environmental problems is hydrocarbon pollution. Hydrocarbons are mostly the result of petroleum based activities. Anthropogenic activities, natural seepage and accidental spills are of particular interest in the environmental quality. The health effects of these chemicals are widely known. In the hour of alarming pollution by these hydrocarbons, a newer, cheaper, and safer technology is needed for cleanup, moving beyond the conventional mechanical and chemical methods, which are not only expensive but ineffective also. Bioremediation is a promising technology, functioning on complete mineralization of contaminants by the diverse metabolic processes owned by microorganisms. Many indigenous and genetically modified bacteria are capable of crude oil degradation. This paper presents an updated overview of petroleum hydrocarbon degradation by bacteria.
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49

Pegu, Ajanta. "Hydrocarbon Source Rock Analysis of Barail & Disang group." International Journal for Research in Applied Science and Engineering Technology 10, no. 5 (May 31, 2022): 2305–12. http://dx.doi.org/10.22214/ijraset.2022.42814.

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Abstract: Petroleum source rocks are those which has sufficient amount of organic matter to generate and expel hydrocarbons to form a commercial accumulation of oil or gas. The objective of this project is to analyze the principal learning on the application of the formation of petroleum source rocks and hydrocarbon generation to exploration activities along with evaluation of petroleum source rocks and hydrocarbon generation. In this project, samples of Barail Group and Disang Group of rocks of Naga-Schuppen Belt were analyzed to determine their source rock characteristics and petroleum generative potentials Keywords: source rock, van Krevelen, Rock Eval pyrolysis, Hydrogen Index, Total organic Carbon content
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50

Borazjani, S., D. Kulikowski, K. Amrouch, and P. Bedrikovetsky. "Composition changes of hydrocarbons during secondary petroleum migration." APPEA Journal 58, no. 2 (2018): 784. http://dx.doi.org/10.1071/aj17127.

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We investigate secondary migration of hydrocarbons with significant composition difference between the source and oil pools in the Cooper-Eromanga Basin, Australia. The secondary migration period is significantly shorter than the time of the hydrocarbon pulse generation, so neither adsorption nor dispersion of components can explain the concentration difference. The filtration coefficients, obtained from oil compositions in source rock (Patchawarra Formation) and in the reservoir (Poolowanna Formation and Hutton Sandstone), monotonically increase as carbon number increases. The monotonicity takes place for heavy hydrocarbons (n > 10). Loss of monotonicity for light and intermediate hydrocarbons can be explained by their evaporation into the gas phase. The evaporation of light and intermediate hydrocarbons into the gas phase is supported by their concentrations in oil, which are higher in source rock than in trapped reservoir oil. The paper proposes deep bed filtration of hydrocarbons with component kinetic retention by the rock. Introduction of the component capture rate into the mass balance transport equation allows matching the concentration difference, and the tuned filtration coefficients are in the common range. The results suggest that deep bed filtration controls the final reservoir oil composition during secondary migration in the Cooper-Eromanga Basin petroleum system, which was not previously considered.
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