Academic literature on the topic 'Petroleum - Microbial'

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Journal articles on the topic "Petroleum - Microbial"

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Li, Dongmei, and Philip Hendry. "Microbial diversity in petroleum reservoirs." Microbiology Australia 29, no. 1 (2008): 25. http://dx.doi.org/10.1071/ma08025.

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Buried hydrocarbon deposits, such as liquid petroleum, represent an abundant source of reduced carbon for microbes. It is not surprising therefore that many organisms have adapted to an oily, anaerobic life deep underground, often at high temperatures and pressures, and that those organisms have had, and in some cases continue to have, an effect on the quality and recovery of the earth?s diminishing petroleum resources. There are three key microbial processes of interest to petroleum producers: reservoir souring, hydrocarbon degradation and microbially enhanced oil recovery (MEOR).
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Varjani, Sunita J. "Microbial degradation of petroleum hydrocarbons." Bioresource Technology 223 (January 2017): 277–86. http://dx.doi.org/10.1016/j.biortech.2016.10.037.

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Sui, Xin, Xuemei Wang, Yuhuan Li, and Hongbing Ji. "Remediation of Petroleum-Contaminated Soils with Microbial and Microbial Combined Methods: Advances, Mechanisms, and Challenges." Sustainability 13, no. 16 (August 18, 2021): 9267. http://dx.doi.org/10.3390/su13169267.

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The petroleum industry’s development has been supported by the demand for petroleum and its by-products. During extraction and transportation, however, oil will leak into the soil, destroying the structure and quality of the soil and even harming the health of plants and humans. Scientists are researching and developing remediation techniques to repair and re-control the afflicted environment due to the health risks and social implications of petroleum hydrocarbon contamination. Remediation of soil contamination produced by petroleum hydrocarbons, on the other hand, is a difficult and time-consuming job. Microbial remediation is a focus for soil remediation because of its convenience of use, lack of secondary contamination, and low cost. This review lists the types and capacities of microorganisms that have been investigated to degrade petroleum hydrocarbons. However, investigations have revealed that a single microbial remediation faces difficulties, such as inconsistent remediation effects and substantial environmental consequences. It is necessary to understand the composition and source of pollutants, the metabolic genes and pathways of microbial degradation of petroleum pollutants, and the internal and external aspects that influence remediation in order to select the optimal remediation treatment strategy. This review compares the degradation abilities of microbial–physical, chemical, and other combination remediation methods, and highlights the degradation capabilities and processes of the greatest microbe-biochar, microbe–nutrition, and microbe–plant technologies. This helps in evaluating and forecasting the chemical behavior of contaminants with both short- and long-term consequences. Although there are integrated remediation strategies for the removal of petroleum hydrocarbons, practical remediation remains difficult. The sources and quantities of petroleum pollutants, as well as their impacts on soil, plants, and humans, are discussed in this article. Following that, the focus shifted to the microbiological technique of degrading petroleum pollutants and the mechanism of the combined microbial method. Finally, the limitations of existing integrated microbiological techniques are highlighted.
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Wang, Jing, and Jiti Zhou. "The effects of offshore petroleum exploitation on microbial community and antibiotic resistome of adjacent marine sediments." Water Science and Technology 81, no. 12 (June 15, 2020): 2501–10. http://dx.doi.org/10.2166/wst.2020.289.

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Abstract The exploitation of petroleum in offshore areas is becoming more prosperous due to the increasing human demand for oil. However, the effects of offshore petroleum exploitation on the microbial community in the surrounding environment are still not adequately understood. In the present study, variations in the composition, function, and antibiotic resistance of the microbial community in marine sediments adjacent to an offshore petroleum exploitation platform were analyzed by a metagenomics-based method. Significant shifts in the microbial community composition were observed in sediments impacted by offshore petroleum exploitation. Nitrosopumilales was enriched in marine sediments with the activities of offshore petroleum exploitation compared to the control sediments. The abundances of function genes involved in carbon, butanoate, methane, and fatty acid metabolism in sediment microbial communities also increased due to the offshore petroleum exploitation. Offshore petroleum exploitation resulted in the propagation of some antibiotic resistance genes (ARGs), including a multidrug transporter, smeE, and arnA, in marine sediments via horizontal gene transfer mediated by class I integrons. However, the total abundance and diversity of ARGs in marine sediments were not significantly affected by offshore petroleum exploitation. This study is the first attempt to analyze the impact of offshore petroleum exploitation on the spread of antibiotic resistance.
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Wang, Ji Hua, and Shan Shan Zhang. "The Application of Microbes in Petroleum Industry." Advanced Materials Research 868 (December 2013): 542–46. http://dx.doi.org/10.4028/www.scientific.net/amr.868.542.

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With the advances in biological sciences, microbiology techniques to be applied to people in all areas of production and life, this paper introduces the microorganisms in the oil industry in all sectors such as oil and gas exploration microorganisms, microbial enhanced oil recovery and microbial degradation of the oil pollution and other aspects of the application. By summarizing the impact of microbial technology for the various aspects of oil industry, make the foundation of the microbial creative application in the field of oil industry.
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Maruthamuthu, Sundaram, Baskaran Dinesh Kumar, Shanmugavel Ramachandran, Balakrishnan Anandkumar, Seeni Palanichamy, Maruthai Chandrasekaran, Palani Subramanian, and Narayanan Palaniswamy. "Microbial Corrosion in Petroleum Product Transporting Pipelines." Industrial & Engineering Chemistry Research 50, no. 13 (July 6, 2011): 8006–15. http://dx.doi.org/10.1021/ie1023707.

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Sen, Ramkrishna. "Biotechnology in petroleum recovery: The microbial EOR." Progress in Energy and Combustion Science 34, no. 6 (December 2008): 714–24. http://dx.doi.org/10.1016/j.pecs.2008.05.001.

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Adkins, Jon P., Laura A. Cornell, and Ralph S. Tanner. "Microbial composition of carbonate petroleum reservoir fluids." Geomicrobiology Journal 10, no. 2 (April 1992): 87–97. http://dx.doi.org/10.1080/01490459209377909.

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Banks, M. Katherine, Hadessa Mallede, and Karrie Rathbone. "Rhizosphere Microbial Characterization in Petroleum-Contaminated Soil." Soil and Sediment Contamination: An International Journal 12, no. 3 (May 2003): 371–85. http://dx.doi.org/10.1080/713610978.

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Joshi, Madhvi N., Shivangi V. Dhebar, Shivani V. Dhebar, Poonam Bhargava, Aanal Pandit, Riddhi P. Patel, Akshay Saxena, and Snehal B. Bagatharia. "Metagenomics of petroleum muck: revealing microbial diversity and depicting microbial syntrophy." Archives of Microbiology 196, no. 8 (May 17, 2014): 531–44. http://dx.doi.org/10.1007/s00203-014-0992-0.

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Dissertations / Theses on the topic "Petroleum - Microbial"

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Morais, Daniel Kumazawa. "Petroleum effects on soil microbial communities." Universidade Federal de Viçosa, 2015. http://www.locus.ufv.br/handle/123456789/8468.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
O petróleo é a principal fonte de energia no Brasil, onde o consumo de óleo continua subindo desde 2013, atingindo atualmente 2.2% do total de energia consumida no mundo. A descoberta recente de petróleo nas baias do Espirito Santo, Campos e Santos, pode representar uma excelente oportunidade para atender as demandas energéticas nacionais. Entretanto, a exploração de petróleo oferece riscos a microbiota e toda a vida marinha. Microrganismos são responsáveis pela ciclagem de nutrientes, podem degradar compostos orgânicos recalcitrantes e muitas espécies são reportadas como sensíveis à contaminação por hidrocarbonetos do petróleo. Esse trabalho teve o objetivo de avaliar as alterações na comunidade microbiana em solos sob a contaminação por petróleo e avaliar os efeitos do Co-produto de biodiesel (BCP) como um agente protetor da microbiota do solo perante a adição de petróleo. Foram utilizados solos da Ilha da Trindade, e da estação de pesquisa Highfield no Rothamsted Research, UK. Foram montados microcosmos com 20 gramas de solo e os tratamentos utilizaram petróleo intemperizado. Os solos foram incubados a 26° C com correção da umidade para cerca de 60% da capacidade de retenção de água dos solos. Foi utilizada a medição de evolução de CO2 para avaliar a atividade do solo, durante o período de incubação, e a extração de DNA genômico do solo, ao final do período de incubação, para avaliar as mudanças nas comunidades microbianas dos tratamentos e controles. O DNA foi submetido para o sequenciamento de amplicons de 16S rDNA para a avaliação de Bacteria e Archaea e de amplicons da região ITS1 para a avaliação de Fungos utilizando a plataforma Illumina HiSeq. Foi feita a comparação das diversidades alpha e beta e análise das alterações taxonômicas. Essa tese está dividida em dois capítulos. O primeiro descreve os efeitos do petróleo nas comunidades microbianas do solo da Ilha da Trindade. No segundo capítulo foi testado o efeito protetor do BCP sobre a microbiota dos solos da Ilha da Trindade, do campo Bare Fallow e do campo Grassland do Rothamsted Research contra a adição de óleo. O petróleo teve um grande efeito negativo sobre a diversidade microbiana da Ilha da Trindade, mas não mudou a diversidade microbiana dos solos agrícolas do Rothamsted. A comparação taxonômica mostrou aumento do filo Actinobacteria, mudanças em várias classes de Proteobacteria e redução da classe Nitrosphaerales do filo Archaea. Esse é o primeiro esforço para aquisição de conhecimento sobre o efeito da contaminação de solos de uma ilha oceânica brasileira com petróleo. Essa informação é importante para guiar qualquer futura estratégia de biorremediação que se faça necessária.
Crude oil is still the dominant energy source in Brazil where oil consumption keeps rising since 2013, reaching nowadays 2.2% of the world‟s energy consumption. A recent discovery of crude oil reservoirs at the Espirito Santo, Campos and Santos basins, can represent an excellent opportunity to meet the country‟s economic and energetic demands. However, offshore exploration offers risks to the microbiota and the whole sea life. Microbes are responsible for nutrient cycling can degrade recalcitrant organic compounds and several species have been reported as sensitive to petroleum hydrocarbons. This work aimed to evaluate microbial community shifts in soils under crude oil contamination and assess the effects of Biodiesel co-product (BCP) as a protecting agent of soil microbiota under crude oil addition. We used soils from the Trindade Island and from the Highfield research station at Rothamsted Research, UK. We assembled microcosms of 20 grams and contaminated the soils using weathered crude oil. Soils were incubated at 26° C with moisture correction to ca. 60% water holding capacity. We used CO2 evolution measurements to evaluate soil activity, during the incubation, and soil genomic DNA extraction, at the end of incubation period, to evaluate microbial community changes from treatments and controls. DNA was submitted to amplicon sequencing of 16S rDNA for Bacteria and Archaea and the ITS1 region for Fungi using Illumina MiSeq platform. We compared alpha and beta-diversity and taxonomic shifts. This thesis is divided in two chapters. The first describes the effects of crude oil on Trindade Island‟s soil microbial communities. In the second chapter we tested the protective effects of BCP on Trindade Island, Rothamsted‟s Bare Fallow and Grassland soils, against the amendment with crude oil. Crude oil had a major negative effect on microbial diversity for Trindade Island, but didn‟t change the diversity of Rothamsted agricultural soils. Taxonomy comparisons showed rise of the Actinobacteria phylum, shifts in several Proteobacteria classes and reduction of the Archaea class Nitrososphaerales. This is the first effort in acquiring knowledge concerning the effect of crude oil contamination in soils of a Brazilian oceanic island. This information is important to guide any future bioremediation strategy that can be required.
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Adelaja, O. "Bioremediation of petroleum hydrocarbons using microbial fuel cells." Thesis, University of Westminster, 2015. https://westminsterresearch.westminster.ac.uk/item/9qvyy/bioremediation-of-petroleum-hydrocarbons-using-microbial-fuel-cells.

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Environmental pollution by petroleum hydrocarbons has serious environmental consequences on critical natural resources upon which all living things (including mankind) largely depend. Microbial fuel cells (MFCs) could be employed in the treatment of these environmental pollutants with concomitant bioelectricity generation. Therefore, the overarching objective of this study was to develop an MFC system for the effective and efficient treatment of petroleum hydrocarbons in both liquid and particulate systems. Biodegradation of target hydrocarbons, phenanthrene and benzene, was investigated in dual-chambered microbial fuel cells (MFCs) using different inoculum types - Shewanella oneidensis MR1 14063, Pseudomonas aeruginosa NCTC 10662, mixed cultures and their combinations thereof. All the inocula showed high potentials for phenanthrene and benzene degradation in liquid systems with a minimum degradation efficiency of 97 % and 86 % respectively with concomitant power production (up to 1.25 mWm-2). The performance of MFCs fed with a mixture of phenanthrene and benzene under various operating conditions - temperature, substrate concentration, addition of surfactants and cathodic electron acceptor type – was investigated. The interaction effects of three selected operating parameters - external resistance, salinity and redox mediator were also investigated using response surface methodology. The outcomes of this study demonstrated the robustness of MFCs with good degradation performance (range 80 - 98 %) and maximum power production up to 10 mWm-2 obtained at different treatment conditions. Interactive effects existed among the chosen independent factors with external resistance having a significant impact on MFC performance, with maximum power output of 24 mWm-2 obtained at optimised conditions - external resistance (69.80 kΩ) , redox mediator (29.30μM, Riboflavin) and salinity (1.3 % w/v NaCl). The treatment of a mixture of phenanthrene and benzene using two different tubular MFCs designed for both in situ and ex situ applications in aqueous systems was investigated over long operational periods (up to 155 days). The outcomes of this work demonstrated stable MFC performance at harsh nutrient conditions and ambient temperatures. Simultaneous removal of petroleum hydrocarbons (> 90 %) and bromate, used as catholyte, (up to 79 %) with concomitant biogenic electricity generation (i.e. peak power density up to 6.75 mWm-2) were observed. The performance of a tubular MFC system in phenanthrene-contaminated soil was investigated in the last study. The outcomes of this work has demonstrated the simultaneous removal of phenanthrene (86%) and bromate (95%) coupled with concomitant bioelectricity generation (about 4.69 mWm-2) using MFC systems within a radius of influence (ROI) up to 8 cm. The overall outcomes of this study suggest the possible application of MFC technology in the effective treatment of petroleum hydrocarbons contaminated groundwater or industrial effluents and soil systems (mostly in subsurface environments), with concomitant energy recovery. MFC technology could potentially be utilised as an independent system in lieu of other bioremediation technologies (e.g. pump and treat, electrobioremediation or permeable reactive barriers) or integrated with existing infrastructure such as monitoring wells or piezometers.
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Phillips, Pamela June. "Microbial degradation of hydrocarbons in aqueous systems." Thesis, University of Surrey, 2003. http://epubs.surrey.ac.uk/842666/.

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There is a vast worldwide consumption of petroleum hydrocarbons and accidental release in to the environment is common. For example petroleum forecourt retail outlets have 'interceptors' to prevent release of hydrocarbons into the environment. The aim of this study was to investigate options for in-situ bioremediation of the hydrocarbon substrates within these 'interceptors' in a laboratory model. The initial studies on bioremediation were undertaken with diesel as the substrate. It was shown that the addition of nitrogen and phosphorus to the system increased hydrocarbon mineralisation by a factor of 16, resulting in increased carbon dioxide evolution. There was strong evidence indicating that nitrogen and phosphorus were the limiting factor for hydrocarbon metabolism in this aqueous system. Trichoderma harzianum and a soil bacterial isolate LFC D1 FI were assessed and shown to degrade hexadecane and pristane. The positive affect of adding a cosubstrate was evident in flask studies; the rates of degradation by LFC D1 FI and T. harzianum were approximately doubled and tripled respectively in the presence of glucose compared to treatments without glucose. Previous attention has focused on the ability of Phanerochaete chrysosporium to degrade polycyclic aromatic hydrocarbons; in this study the degradation of aliphatics was investigated. Spores from P. chrysosporium induced on the hydrocarbon substrate were found to be necessary to degrade hexadecane. Pseudomonas putida was unable to grow in liquid media containing hydrocarbons, however on solid media and in an aqueous environment containing acid-washed sand, degradation of hydrocarbons was evident, hi the presence of sand P. putida degraded both hexadecane and pristane by 70% of the initial concentration added; in the absence of sand no degradation in the aqueous system was seen. This suggests surface attachment plays an important role in hydrocarbon degradation by P. putida. The attachment and use of the sessile P. putida in aliphatic hydrocarbon degradation is discussed.
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Silva, Tiago Rodrigues e. "Caracterização polifásica da microbiota presente em amostras de petróleo de reservatórios brasileiros." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317328.

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Orientador: Valéria Maia Merzel
Dissertação (mestrado) - Universidade Estadual de Campinas, Insituto de Biologia
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Resumo: Estudos realizados em reservatórios de petróleo têm evidenciado que parte da microbiota associada a este tipo de ambiente é representada por bactérias e arqueias de distribuição geográfica bastante ampla e que diversos destes organismos têm potencial para transformar compostos orgânicos e inorgânicos, atuando na interface óleo-água dos reservatórios. A investigação de micro-organismos com potencial para biodeterioração, biodegradação e biocorrosão encontrados em depósitos petrolíferos é de grande importância, uma vez que estes organismos podem estar relacionados com a perda da qualidade do petróleo nos reservatórios e etapas subseqüentes de exploração. Este estudo teve como finalidade comparar a microbiota presente em amostras de óleo de dois poços de petróleo terrestres da Bacia Potiguar (RN), identificados como GMR75 (poço biodegradado) e PTS1 (poço não-biodegradado). As comunidades microbianas foram estudadas usando técnicas de cultivo (enriquecimentos microbianos e isolamento) e independentes de cultivo (construção de bibliotecas de genes RNAr 16S). Os micro-organismos cultivados de ambos os poços mostraram-se afiliados aos filos Actinobacteria, Firmicutes e Proteobacteria. As bibliotecas de gene RNAr 16S foram construídas a partir de DNA total extraído do petróleo bruto. Ambas as bibliotecas de bactérias revelaram uma grande diversidade, com 8 filos diferentes para o poço GMR75, Actinobacteria, Bacteroidetes, Deferribacteres, Spirochaetes, Firmicutes, Proteobacteria, Thermotoga e Synergistetes, e 5 filos para o poço PTS1, Actinobacteria, Chloroflexi, Firmicutes, Proteobacteria e Thermotogae. A biblioteca de genes RNAr 16S de arqueias só foi obtida para o poço GMR75 e todos os clones encontrados mostraram-se relacionados a membros da ordem Methanobacteriales. Os resultados de diversidade sugerem que a metanogênese é o processo terminal dominante no poço, o que indica uma biodegradação anaeróbia. A comparação dos estudos dependente e independente de cultivo mostrou que alguns gêneros, como Janibacter, Georgenia, Saccharopolyspora, Tessaracoccus, Brevundimonas e Brachymonas não foram encontradas na abordagem independente de cultivo, sugerindo que mais clones devam ser seqüenciados para cobrir toda a diversidade presente na amostra. Nossa hipótese de que poderia haver algum agente antimicrobiano inibindo o crescimento de bactérias degradadoras de hidrocarbonetos no poço não-biodegradado não foi confirmada. No entanto, durante os testes realizados, uma bactéria, Bacillus pumilus, isolada em estudos anteriores de reservatórios da Bacia de Campos, apresentou resultados positivos de inibição para todas as linhagens testadas como indicadoras, e os testes de caracterização do composto revelaram ser este um diterpeno da classe das Ciatinas.
Abstract: Recent studies from oil fields have shown that microbial diversity is represented by bacteria and archaea of wide distribution, and that many of these organisms have potential to metabolize organic and inorganic compounds. The potential of biodeterioration, biodegradation and biocorrosion by microorganisms in oil industry is of great relevance, since these organisms may be related with the loss of petroleum quality and further exploration steps. The aim of the present study was to compare the microbial communities present in two samples from terrestrial oil fields from Potiguar basin (RN - Brazil), identified as GMR75 (biodegraded oil) and PTS1 (non-biodegraded oil). Microbial communities were investigated using cultivation (microbial enrichments and isolation) and molecular approaches (16S rRNA gene clone libraries). The cultivated microorganisms recovered from both oil-fields were affiliated with the phyla Actinobacteria, Firmicutes and Proteobacteria. The 16S rRNA gene clone libraries were constructed from metagenomic DNA obtained from crudeoil. Both bacterial libraries revealed a great diversity, encompassing representatives of 8 different phyla for GMR75, Actinobacteria, Bacteroidetes, Deferribacteres, Spirochaetes, Firmicutes, Proteobacteria, Thermotogae and Synergistetes, and of 5 different phyla, Actinobacteria, Chloroflexi, Firmicutes, Proteobacteria and Thermotoga, for PTS1. The archaeal 16S rRNA clone library was obtained only for GMR75 oil and all phylotypes were affiliated with order Methanobacteriales. Diversity resuts suggest that methanogenesis is the dominant terminal process in GMR75 reservoir, driven by anaerobic biodegradation. The cross-evaluation of culture-dependent and independent techniques indicates that some bacterial genera, such as Janibacter, Georgenia, Saccharopolyspora, Tessaracoccus, Brevundimonas and Brachymonas, were not found using the the 16S rRNA clone library approach, suggesting that additional clones should be sequenced in order to cover diversity present in the sample. Our hypothesis that biodegrading bacterial populations could be inhibited by antimicrobialproducing microorganisms in the non biodegraded oil field (PTS1) was not confirmed. However, one Bacillus pumilus strain, previously isolated from Campos Basin reservoirs, showed positive results in inhibitory tests for all indicator strains. Chemical analyses allowed us to identify the compound as a diterpen from the Cyathin class.
Mestrado
Genetica de Microorganismos
Mestre em Genética e Biologia Molecular
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Kropp, Kevin Glen. "Aerobic microbial metabolism of condensed thiophenes found in petroleum." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq23009.pdf.

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Zhang, Zhengming. "Microbial oxidation of inorganic sulfide in sour water /." Access abstract and link to full text, 1989. http://0-wwwlib.umi.com.library.utulsa.edu/dissertations/fullcit/9013729.

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Maila, M. P. "Microbial ecology and bio-monitoring of total petroleum contaminated soil environments." Pretoria : [s.n.], 2004. http://upetd.up.ac.za/thesis/available/etd-02092006-100257.

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Wei, Ren, and Wolfgang Zimmermann. "Microbial enzymes for the recycling of recalcitrant petroleum-based plastics: how far are we?" Universität Leipzig, 2017. https://ul.qucosa.de/id/qucosa%3A21103.

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Petroleum-based plastics have replaced many natural materials in their former applications. With their excellent properties, they have found widespread uses in almost every area of human life. However, the high recalcitrance of many synthetic plastics results in their long persistence in the environment, and the growing amount of plastic waste ending up in landfills and in the oceans has become a global concern. In recent years, a number of microbial enzymes capable of modifying or degrading recalcitrant synthetic polymers have been identified. They are emerging as candidates for the development of biocatalytic plastic recycling processes, by which valuable raw materials can be recovered in an environmentally sustainable way. This review is focused on microbial biocatalysts involved in the degradation of the synthetic plastics polyethylene, polystyrene, polyurethane and polyethylene terephthalate (PET). Recent progress in the application of polyester hydrolases for the recovery of PET building blocks and challenges for the application of these enzymes in alternative plastic waste recycling processes will be discussed.
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Grassia, Gino Sebastian, and n/a. "The isolation, growth and survival of thermophilic bacteria from high temperature petroleum reservoirs." University of Canberra. Applied Science, 1995. http://erl.canberra.edu.au./public/adt-AUC20060712.131412.

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The microbial ecology of 45 high temperature (> 50 ° C) petroleum reservoirs was investigated by isolating and characterizing bacteria that were present in their produced fluids. Initial work was aimed at selecting a suitable high temperature petroleum reservoir for the study of natural microbial populations. Experimental work then focussed on establishing the physico-chemical conditions that prevail in the selected reservoir and on developing media and enrichment conditions for the isolation of microorganisms indigenous to the reservoir. The ability of reservoir bacteria to grow and survive under the physical and chemical conditions found in the selected reservoir was used to assess the likelihood of an indigenous origin for these bacteria. The petroleum reservoir selected for study was the Alton petroleum reservoir (SW Queensland, Australia). It was established that most of the physico-chemical conditions in the Alton reservoir had remained unchanged since oil recovery began. The stability of redox conditions (90 mV) in the reservoir over its operating life was identified as an important factor in the coexistence of strict aerobic and strict anaerobic bacterial populations within the reservoir. An important change that has occurred in the Alton reservoir over its operating life because of oil recovery was an increase in water pH from 6.41 to 8.42 as a result of carbon dioxide loss (1.36 atm to 0.0134 atm) from the reservoir. Development of novel enrichment procedures that simulated Alton reservoir conditions led to the isolation of previously unreported aerobic and anaerobic populations of thermophilic bacteria. The aerobic bacteria isolated were identified as either endosporeforming heterotrophic bacteria from the genus Bacillus or nonspore-forming heterotrophic bacteria resembling members of the genus Thermoleophilum. All aerobes grew on carbon sources such as acetate and n-heptadecane that are normal constituents of the reservoir. The anaerobic bacteria isolated were characterized as sheathed fermentative bacteria from the order Thermotogales or non-sheathed fermentative bacteria. In parallel studies, the natural microbial populations in other reservoirs were investigated and I concluded that fermentative microorganisms were common inhabitants of high temperature petroleum reservoirs. The isolation of fermentative bacteria from these high temperature petroleum reservoirs established that fermentative bacteria are a fourth major microbial group, together with hydrocarbon-oxidizers, sulphate-reducers and methanogens, to be reported in petroleum reservoirs. The fermentative bacteria use organic nutrients and carbohydrates, but not contemporary crude oil as the principal nutrient source within reservoir waters. The thermophilic bacteria isolated from Alton petroleum reservoir demonstrated growth characteristics such as temperature (optima 50-70 ° C and range 37-85 ° C), pH (optima 6.0-9.0 and range 5.0-9.0 and salinity (optima 0-15 g per litre and range 0-30 g per litre), that were consistent with conditions encountered in the Alton reservoir (temperature 75 � C, pH 8.5 and TDS 2.7 g per litre). The isolated bacteria also demonstrated a number of characteristics that might enable them to survive adverse conditions that could be encountered in a petroleum reservoir environment. The characteristics that contribute to aerobic bacteria surviving in and overcoming periods of oxygen limitation include well-documented processes such as sporulation, by Bacillus spp., and microaerophily. The characteristics that contribute to fermentative bacteria surviving were: (1) a natural tolerance to reservoir physico-chemical fluctuations, (2) an ability to remain viable when metabolic activity was suppressed to very low rates by the growth-limiting conditions imposed, and (3) possible formation of viable ultramicrobacteria (UMB). Formation of UMB (bacteria smaller than 0.3 |im) by thermophilic bacteria has not been reported previously. The recovery of thermophilic UMB by filtration from the Alton reservoir water indicates that these bacteria occur in natural habitats. This study found the formation of thermophilic UMB and their survival characteristics differed considerably from that reported for the mesophilic, marine bacterium Vibrio sp. DWI. Unlike mesophilic marine bacteria, thermophilic bacteria did not always respond to nutrient deprivation by forming UMB and that these UMB did not show any increased ability to survive in the face of adverse conditions. Although the formation of UMB as part of routine cell growth and division was not demonstrated directly in this study, circumstantial evidence suggests that they form part of a natural life cycle. The exact conditions that result in UMB formation and their role in survival remain unresolved. The capacity of nonspore-forming indigenous populations from Alton to survive sudden shifts in environmental conditions that might result from common oilfield operations was poor. Such operations were demonstrated to be inhibitory or lethal to Alton reservoir bacteria. It also was concluded that such oilfield operations suppress indigenous microbiota. However, the impacts of most oilfield operations within a reservoir are likely to be confined to the immediate area surrounding injection and producing wells. Minimizing the localized effects of oilfield practices on indigenous reservoir populations will lead to the better management of undesirable microbial activity in reservoirs such as H2S formation (souring) and facilitate development of better microbially mediated oil recovery process. This study showed that selected reservoir isolates possess characteristics which are suitable for in situ biotechnological applications such as microbially enhanced oil recovery (MEOR). Characteristics favourable for enhanced oil recovery include a capability for UMB formation, which would enable better dispersion, and resistance to high concentrations of reservoir components such as calcium, magnesium, strontium, heavy metals and hydrocarbons.
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Singh, Gargi. "Influence of Petroleum Deposit Geometry on Local Gradient of Electron Acceptors and Microbial Catabolic Potential." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/31431.

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A field survey was conducted following the Deepwater Horizon blowout and it was noted that resulting coastal petroleum deposits possessed distinct geometries, ranging from small tar balls to expansive horizontal oil sheets. A laboratory study evaluated the effect of oil deposit geometry on localized gradients of electron acceptors and microbial community composition, factors that are critical to accurately estimating biodegradation rates. One-dimensional top-flow sand columns with 12-hour simulated tidal cycles compared two contrasting geometries (isolated tar â ballsâ versus horizontal â sheetsâ ) relative to an oil-free control. Significant differences in the effluent dissolved oxygen and sulfate concentrations were noted among the columns, indicating presence of anaerobic zones in the oiled columns, particularly in the sheet condition. Furthermore, quantification of genetic markers of electron acceptor and catabolic conditions via quantitative polymerase chain reaction of dsrA (sulfate-reduction), mcrA (methanogenesis), and cat23 (oxygenation of aromatics) genes in column cores suggested more extensive anaerobic conditions induced by the sheet relative to the ball geometry. Denaturing gradient gel electrophoresis similarly revealed that distinct gradients of bacterial communities established in response to the different geometries. Thus, petroleum deposit geometry impacts local redox and microbial characteristics and may be a key factor for advancing attenuation models and prioritizing cleanup.
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Books on the topic "Petroleum - Microbial"

1

Braddock, Joan F. Petroleum hydrocarbon-degrading microbial communities in Beaufort-Chukchi Sea sediments. Fairbanks, AK: Coastal Marine Institute, School of Fisheries and Ocean Sciences, University of Alaska Fairbanks, 2004.

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Braddock, Joan F. Petroleum hydrocarbon-degrading microbial communities in Beaufort-Chukchi Sea sediments. Fairbanks, AK: Coastal Marine Institute, School of Fisheries and Ocean Sciences, University of Alaska Fairbanks, 2004.

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Song, Hong-Gyu. Petroleum hydrocarbons in soil: Biodegradation and effects on the microbial community. Ann Arbor, Mich: U.M.I. Dissertation Information Service, 1990.

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Spain, Jim C. Biodegradation of jet fuel by aquatic microbial communities. Gulf Breeze, Fla: Environmental Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, 1985.

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Corinne, Whitby, and Skovhus Torben Lund, eds. Applied microbiology and molecular biology in oilfield systems: Proceedings from the International Symposium on Applied Microbiology and Molecular Biology in Oil Systems (ISMOS-2), 2009. Dordrecht: Springer Science+Business Media, 2011.

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1978-, Wei Li, ed. You tian liu suan yan huan yuan jun fen zi sheng tai xue ji qi huo xing sheng tai tiao kong yan jiu: Research on molecular ecology and activities regulation of oilfield sulfate reducing bacteria. Beijing: Ke xue chu ban she, 2009.

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Thorpe, J. W. Microbial degradation of hydrocarbon mixtures in a marine sediment under different temperature regimes. Ottawa: Published under auspices of Environmental Studies Research Funds [by] Nova Scotia Research Foundation Corporation, 1987.

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Ismail, Wael A., Jonathan D. Van Hamme, John J. Kilbane, and Ji-Dong Gu, eds. Petroleum Microbial Biotechnology: Challenges and Prospects. Frontiers Media SA, 2017. http://dx.doi.org/10.3389/978-2-88945-256-9.

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Premuzic, Eugene T. Microbial Enhancement of Oil Recovery - Recent Advances: Proceedings of the 1992 International Conference on Microbial Enhanced Oil Recovery (Developments in Petroleum Science). Elsevier Publishing Company, 1993.

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Britain), Energy Institute (Great, ed. Guidelines for the investigation of the microbial content of petroleum fuels and for the implementation of avoidance and remedial strategies. 2nd ed. London: Energy Institute, 2008.

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Book chapters on the topic "Petroleum - Microbial"

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Katz, Barry Jay. "Microbial Gas." In Encyclopedia of Petroleum Geoscience, 1–2. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-319-02330-4_91-1.

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Ron, Eliora Z. "Microbial Life on Petroleum." In Journey to Diverse Microbial Worlds, 303–15. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-011-4269-4_21.

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Magot, Michel. "Indigenous Microbial Communities in Oil Fields." In Petroleum Microbiology, 21–33. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817589.ch2.

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Dellagnezze, Bruna Martins, Milene Barbosa Gomes, and Valéria Maia de Oliveira. "Microbes and Petroleum Bioremediation." In Microbial Action on Hydrocarbons, 97–123. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1840-5_5.

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Mahmoud, Ghada Abd-Elmonsef, and Magdy Mohmed Khalil Bagy. "Microbial Degradation of Petroleum Hydrocarbons." In Microbial Action on Hydrocarbons, 299–320. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1840-5_12.

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Alegbeleye, Oluwadara Oluwaseun. "Petroleum Microbiology Under Extreme Conditions." In Microbial Action on Hydrocarbons, 441–84. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1840-5_18.

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Effendi, Agus Jatnika, Edwan Kardena, and Qomarudin Helmy. "Biosurfactant-Enhanced Petroleum Oil Bioremediation." In Microbial Action on Hydrocarbons, 143–79. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1840-5_7.

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Morgan, Philip, and Robert J. Watkinson. "Biodegradation of components of petroleum." In Biochemistry of microbial degradation, 1–31. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-1687-9_1.

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Jayasena, Sharmila, and Madushika Perera. "Microbial Bioremediation of Petroleum Hydrocarbons." In Microbial Rejuvenation of Polluted Environment, 263–91. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-7447-4_11.

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Sunde, Egil, and Terje Torsvik. "Microbial Control of Hydrogen Sulfide Production in Oil Reservoirs." In Petroleum Microbiology, 199–213. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817589.ch10.

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Conference papers on the topic "Petroleum - Microbial"

1

Philippovich, N., and J. Winter. "Microbial Clarification for Treating Reinjected Oilfield Brine." In European Petroleum Conference. Society of Petroleum Engineers, 1998. http://dx.doi.org/10.2118/50622-ms.

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Zekri, Abdulrazag Y., and A. Al-Khanbashi. "Microbial Phase Behavior Laboratory Studies." In Abu Dhabi International Petroleum Exhibition and Conference. Society of Petroleum Engineers, 2000. http://dx.doi.org/10.2118/87294-ms.

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Hoxha, Gazmend, Clara Di Iorio, and Francesca De Ferra. "Microbial Corrosion. New Investigation Techniques." In Abu Dhabi International Petroleum Exhibition and Conference. Society of Petroleum Engineers, 2014. http://dx.doi.org/10.2118/171805-ms.

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Ghadimi, Mohammad Reza, and Mehdy Ardjmand. "Simulation of Microbial Enhanced Oil Recovery." In Abu Dhabi International Petroleum Exhibition and Conference. Society of Petroleum Engineers, 2006. http://dx.doi.org/10.2118/101767-ms.

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Munnecke, D. M., and W. P. Weaver. "Microbial Surface Geochemical Exploration Technology." In Technical Meeting / Petroleum Conference of The South Saskatchewan Section. Petroleum Society of Canada, 1999. http://dx.doi.org/10.2118/99-100.

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Kowalewski, Espen, Ingun Rueslatten, Tony Boassen, Egil Sunde, Jan Age Stensen, Bente-Lise Polden Lillebo, Gunhild Bodtker, and Terje Torsvik. "Analyzing Microbial Improved Oil Recovery Processes From Core Floods." In International Petroleum Technology Conference. International Petroleum Technology Conference, 2005. http://dx.doi.org/10.2523/10924-ms.

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Kowalewski, Espen, Ingun Rueslatten, Tony Boassen, Egil Sunde, Jan Age Stensen, Bente-Lise Polden Lillebo, Gunhild Bodtker, and Terje Torsvik. "Analyzing Microbial Improved Oil Recovery Processes From Core Floods." In International Petroleum Technology Conference. International Petroleum Technology Conference, 2005. http://dx.doi.org/10.2523/iptc-10924-ms.

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Yao, Chuanjin, Guanglun Lei, Jiye Ma, Chuan Wu, and Wenzhong Li. "Experiment and Simulation of Indigenous Microbial Enhanced Oil Recovery (IMEOR)." In International Petroleum Technology Conference. International Petroleum Technology Conference, 2011. http://dx.doi.org/10.2523/iptc-14268-ms.

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Hubert, C., M. Nemati, G. Voordouw, and G. E. Jenneman. "Biogenic Sulfide Production in Continuous Systems: Containment Strategies Targeting Microbial Metabolism." In Canadian International Petroleum Conference. Petroleum Society of Canada, 2002. http://dx.doi.org/10.2118/2002-114-ea.

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Ma, Wencui, Xue-yi You, Xinxin Wang, and Yu Chen. "The Evaluation of Plant-Microbial Remediation of Petroleum Contaminated Soil." In First International Conference on Information Sciences, Machinery, Materials and Energy. Paris, France: Atlantis Press, 2015. http://dx.doi.org/10.2991/icismme-15.2015.93.

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