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1

Li, Dongmei, and Philip Hendry. "Microbial diversity in petroleum reservoirs." Microbiology Australia 29, no. 1 (2008): 25. http://dx.doi.org/10.1071/ma08025.

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Buried hydrocarbon deposits, such as liquid petroleum, represent an abundant source of reduced carbon for microbes. It is not surprising therefore that many organisms have adapted to an oily, anaerobic life deep underground, often at high temperatures and pressures, and that those organisms have had, and in some cases continue to have, an effect on the quality and recovery of the earth?s diminishing petroleum resources. There are three key microbial processes of interest to petroleum producers: reservoir souring, hydrocarbon degradation and microbially enhanced oil recovery (MEOR).
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2

Varjani, Sunita J. "Microbial degradation of petroleum hydrocarbons." Bioresource Technology 223 (January 2017): 277–86. http://dx.doi.org/10.1016/j.biortech.2016.10.037.

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3

Sui, Xin, Xuemei Wang, Yuhuan Li, and Hongbing Ji. "Remediation of Petroleum-Contaminated Soils with Microbial and Microbial Combined Methods: Advances, Mechanisms, and Challenges." Sustainability 13, no. 16 (August 18, 2021): 9267. http://dx.doi.org/10.3390/su13169267.

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The petroleum industry’s development has been supported by the demand for petroleum and its by-products. During extraction and transportation, however, oil will leak into the soil, destroying the structure and quality of the soil and even harming the health of plants and humans. Scientists are researching and developing remediation techniques to repair and re-control the afflicted environment due to the health risks and social implications of petroleum hydrocarbon contamination. Remediation of soil contamination produced by petroleum hydrocarbons, on the other hand, is a difficult and time-consuming job. Microbial remediation is a focus for soil remediation because of its convenience of use, lack of secondary contamination, and low cost. This review lists the types and capacities of microorganisms that have been investigated to degrade petroleum hydrocarbons. However, investigations have revealed that a single microbial remediation faces difficulties, such as inconsistent remediation effects and substantial environmental consequences. It is necessary to understand the composition and source of pollutants, the metabolic genes and pathways of microbial degradation of petroleum pollutants, and the internal and external aspects that influence remediation in order to select the optimal remediation treatment strategy. This review compares the degradation abilities of microbial–physical, chemical, and other combination remediation methods, and highlights the degradation capabilities and processes of the greatest microbe-biochar, microbe–nutrition, and microbe–plant technologies. This helps in evaluating and forecasting the chemical behavior of contaminants with both short- and long-term consequences. Although there are integrated remediation strategies for the removal of petroleum hydrocarbons, practical remediation remains difficult. The sources and quantities of petroleum pollutants, as well as their impacts on soil, plants, and humans, are discussed in this article. Following that, the focus shifted to the microbiological technique of degrading petroleum pollutants and the mechanism of the combined microbial method. Finally, the limitations of existing integrated microbiological techniques are highlighted.
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4

Wang, Jing, and Jiti Zhou. "The effects of offshore petroleum exploitation on microbial community and antibiotic resistome of adjacent marine sediments." Water Science and Technology 81, no. 12 (June 15, 2020): 2501–10. http://dx.doi.org/10.2166/wst.2020.289.

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Abstract The exploitation of petroleum in offshore areas is becoming more prosperous due to the increasing human demand for oil. However, the effects of offshore petroleum exploitation on the microbial community in the surrounding environment are still not adequately understood. In the present study, variations in the composition, function, and antibiotic resistance of the microbial community in marine sediments adjacent to an offshore petroleum exploitation platform were analyzed by a metagenomics-based method. Significant shifts in the microbial community composition were observed in sediments impacted by offshore petroleum exploitation. Nitrosopumilales was enriched in marine sediments with the activities of offshore petroleum exploitation compared to the control sediments. The abundances of function genes involved in carbon, butanoate, methane, and fatty acid metabolism in sediment microbial communities also increased due to the offshore petroleum exploitation. Offshore petroleum exploitation resulted in the propagation of some antibiotic resistance genes (ARGs), including a multidrug transporter, smeE, and arnA, in marine sediments via horizontal gene transfer mediated by class I integrons. However, the total abundance and diversity of ARGs in marine sediments were not significantly affected by offshore petroleum exploitation. This study is the first attempt to analyze the impact of offshore petroleum exploitation on the spread of antibiotic resistance.
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5

Wang, Ji Hua, and Shan Shan Zhang. "The Application of Microbes in Petroleum Industry." Advanced Materials Research 868 (December 2013): 542–46. http://dx.doi.org/10.4028/www.scientific.net/amr.868.542.

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With the advances in biological sciences, microbiology techniques to be applied to people in all areas of production and life, this paper introduces the microorganisms in the oil industry in all sectors such as oil and gas exploration microorganisms, microbial enhanced oil recovery and microbial degradation of the oil pollution and other aspects of the application. By summarizing the impact of microbial technology for the various aspects of oil industry, make the foundation of the microbial creative application in the field of oil industry.
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6

Maruthamuthu, Sundaram, Baskaran Dinesh Kumar, Shanmugavel Ramachandran, Balakrishnan Anandkumar, Seeni Palanichamy, Maruthai Chandrasekaran, Palani Subramanian, and Narayanan Palaniswamy. "Microbial Corrosion in Petroleum Product Transporting Pipelines." Industrial & Engineering Chemistry Research 50, no. 13 (July 6, 2011): 8006–15. http://dx.doi.org/10.1021/ie1023707.

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7

Sen, Ramkrishna. "Biotechnology in petroleum recovery: The microbial EOR." Progress in Energy and Combustion Science 34, no. 6 (December 2008): 714–24. http://dx.doi.org/10.1016/j.pecs.2008.05.001.

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8

Adkins, Jon P., Laura A. Cornell, and Ralph S. Tanner. "Microbial composition of carbonate petroleum reservoir fluids." Geomicrobiology Journal 10, no. 2 (April 1992): 87–97. http://dx.doi.org/10.1080/01490459209377909.

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9

Banks, M. Katherine, Hadessa Mallede, and Karrie Rathbone. "Rhizosphere Microbial Characterization in Petroleum-Contaminated Soil." Soil and Sediment Contamination: An International Journal 12, no. 3 (May 2003): 371–85. http://dx.doi.org/10.1080/713610978.

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10

Joshi, Madhvi N., Shivangi V. Dhebar, Shivani V. Dhebar, Poonam Bhargava, Aanal Pandit, Riddhi P. Patel, Akshay Saxena, and Snehal B. Bagatharia. "Metagenomics of petroleum muck: revealing microbial diversity and depicting microbial syntrophy." Archives of Microbiology 196, no. 8 (May 17, 2014): 531–44. http://dx.doi.org/10.1007/s00203-014-0992-0.

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11

Truskewycz, Adam, Taylor D. Gundry, Leadin S. Khudur, Adam Kolobaric, Mohamed Taha, Arturo Aburto-Medina, Andrew S. Ball, and Esmaeil Shahsavari. "Petroleum Hydrocarbon Contamination in Terrestrial Ecosystems—Fate and Microbial Responses." Molecules 24, no. 18 (September 19, 2019): 3400. http://dx.doi.org/10.3390/molecules24183400.

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Petroleum hydrocarbons represent the most frequent environmental contaminant. The introduction of petroleum hydrocarbons into a pristine environment immediately changes the nature of that environment, resulting in reduced ecosystem functionality. Natural attenuation represents the single, most important biological process which removes petroleum hydrocarbons from the environment. It is a process where microorganisms present at the site degrade the organic contaminants without the input of external bioremediation enhancers (i.e., electron donors, electron acceptors, other microorganisms or nutrients). So successful is this natural attenuation process that in environmental biotechnology, bioremediation has developed steadily over the past 50 years based on this natural biodegradation process. Bioremediation is recognized as the most environmentally friendly remediation approach for the removal of petroleum hydrocarbons from an environment as it does not require intensive chemical, mechanical, and costly interventions. However, it is under-utilized as a commercial remediation strategy due to incomplete hydrocarbon catabolism and lengthy remediation times when compared with rival technologies. This review aims to describe the fate of petroleum hydrocarbons in the environment and discuss their interactions with abiotic and biotic components of the environment under both aerobic and anaerobic conditions. Furthermore, the mechanisms for dealing with petroleum hydrocarbon contamination in the environment will be examined. When petroleum hydrocarbons contaminate land, they start to interact with its surrounding, including physical (dispersion), physiochemical (evaporation, dissolution, sorption), chemical (photo-oxidation, auto-oxidation), and biological (plant and microbial catabolism of hydrocarbons) interactions. As microorganism (including bacteria and fungi) play an important role in the degradation of petroleum hydrocarbons, investigations into the microbial communities within contaminated soils is essential for any bioremediation project. This review highlights the fate of petroleum hydrocarbons in tertial environments, as well as the contributions of different microbial consortia for optimum petroleum hydrocarbon bioremediation potential. The impact of high-throughput metagenomic sequencing in determining the underlying degradation mechanisms is also discussed. This knowledge will aid the development of more efficient, cost-effective commercial bioremediation technologies.
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12

Xu, Rui Dan. "Bioremediation of Petroleum Contaminated Soil." Advanced Materials Research 550-553 (July 2012): 1248–52. http://dx.doi.org/10.4028/www.scientific.net/amr.550-553.1248.

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Two kinds of polyacrylamide(HPAM)-degrading bacteria S1, S2, which can use HPAM as only nitrogen source and the sole carbon source, were isolated from petroleum-contaminated soil of Daqing Oilfield. The bioremediation for treating petroleum contaminated soil by immobilized microorganisms can improve the effect on biodegradation for pollutants in oil fields and reduce the loss of bacteria. The degradation ability of five kinds of embedding immobilization methods on soil pollutant was investigated. The experimental results showed that the immobilized microbial granules, which used polyvinyl alcohols (PVA) and sodium alginate as coagulant, activated carbon as coagulant-support, exhibited good mechanical strength, operated easily, be not breakable and low cost. Experiments results showed that after treatment using this kind of immobilized microbial granules, the HPAM concentration declined from 500 mg•L-1 to 102 mg•L-1 in 48 hours. The degradation rate of HPAM reached 79.6%. At the same time crude oil content decreased from 733.21 mg•L-1 to 9.5 mg•L-1. These immobilized microbial granules can remove 98.7% oil from the petroleum-contaminated soil in 48 hours.
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13

Marshall, Timothy R., and Joseph S. Devinny. "The Microbial Ecosystem in Petroleum Waste Land Treatment." Water Science and Technology 20, no. 11-12 (November 1, 1988): 285–91. http://dx.doi.org/10.2166/wst.1988.0297.

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Microbial populations, microbial activity and environmental conditions in an operating petroleum waste land treatment facility were monitored for eighteen months. Seasonal influences are apparent for both bacterial and fungal populations. During the cooler, wetter seasons, microbe populations were smaller, less variable and inhibited by the adverse environmental conditions. The hotter, drier months supported large, active populations which experienced large swings in numbers and respiratory output. Microenvironments within aggregates were investigated. Analysis of various aggregate sizes revealed differences in population, activity and distribution of microorganisms. Optimization of waste biodegradation in treatment soils requires monitoring the factors affecting the microbial community at the system level and an awareness of the microenvironmental influences.
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14

Van Hamme, Jonathan D., Ajay Singh, and Owen P. Ward. "Recent Advances in Petroleum Microbiology." Microbiology and Molecular Biology Reviews 67, no. 4 (December 2003): 503–49. http://dx.doi.org/10.1128/mmbr.67.4.503-549.2003.

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SUMMARY Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments.
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15

Cruz, Georgiana F. da, Célio F. F. Angolini, Eugênio V. dos Santos Neto, Watson Loh, and Anita J. Marsaioli. "Exopolymeric substances (EPS) produced by petroleum microbial consortia." Journal of the Brazilian Chemical Society 21, no. 8 (2010): 1517–23. http://dx.doi.org/10.1590/s0103-50532010000800016.

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16

Das, Nilanjana, and Preethy Chandran. "Microbial Degradation of Petroleum Hydrocarbon Contaminants: An Overview." Biotechnology Research International 2011 (September 13, 2011): 1–13. http://dx.doi.org/10.4061/2011/941810.

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One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products are of particular concern in the environment. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. Currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. Mechanical and chemical methods generally used to remove hydrocarbons from contaminated sites have limited effectiveness and can be expensive. Bioremediation is the promising technology for the treatment of these contaminated sites since it is cost-effective and will lead to complete mineralization. Bioremediation functions basically on biodegradation, which may refer to complete mineralization of organic contaminants into carbon dioxide, water, inorganic compounds, and cell protein or transformation of complex organic contaminants to other simpler organic compounds by biological agents like microorganisms. Many indigenous microorganisms in water and soil are capable of degrading hydrocarbon contaminants. This paper presents an updated overview of petroleum hydrocarbon degradation by microorganisms under different ecosystems.
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17

Rosenberg, Eugene, Gili Bittan-Banin, Gil Sharon, Avital Shon, Galit Hershko, Itzik Levy, and Eliora Z. Ron. "The phage-driven microbial loop in petroleum bioremediation." Microbial Biotechnology 3, no. 4 (June 24, 2010): 467–72. http://dx.doi.org/10.1111/j.1751-7915.2010.00182.x.

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18

Li, Hong, Xiaokang Li, Tao Yu, Feifei Wang, and Chengtun Qu. "Study on Extreme Microbial Degradation of Petroleum Hydrocarbons." IOP Conference Series: Materials Science and Engineering 484 (March 19, 2019): 012040. http://dx.doi.org/10.1088/1757-899x/484/1/012040.

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19

OHSHIRO, Takashi, and Yoshikazu IZUMI. "Microbial Desulfurization of Organic Sulfur Compounds in Petroleum." Bioscience, Biotechnology, and Biochemistry 63, no. 1 (January 1999): 1–9. http://dx.doi.org/10.1271/bbb.63.1.

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20

Unciano, N. M. "Developing microbial isolates for the biodesulfurization of petroleum." Journal of Biotechnology 150 (November 2010): 90–91. http://dx.doi.org/10.1016/j.jbiotec.2010.08.233.

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21

Tripathi, Nimisha, Raj S. Singh, and Colin D. Hills. "Microbial removal of sulphur from petroleum coke (petcoke)." Fuel 235 (January 2019): 1501–5. http://dx.doi.org/10.1016/j.fuel.2018.08.072.

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22

Bruckberger, Melanie, Deirdre Gleeson, Trevor Bastow, Matthew Morgan, Tom Walsh, John Rayner, Greg Davis, and Geoffrey Puzon. "Unravelling Microbial Communities Associated with Different Light Non-Aqueous Phase Liquid Types Undergoing Natural Source Zone Depletion Processes at a Legacy Petroleum Site." Water 13, no. 7 (March 25, 2021): 898. http://dx.doi.org/10.3390/w13070898.

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Petroleum contaminants are exposed to weathering when released into environment, resulting in the alteration of their chemical composition. Here, we investigated microbial communities through the soil profile at an industrial site, which was exposed to various petroleum products for over 50 years. The petroleum is present as light non-aqueous phase liquid (LNAPL) and is undergoing natural source zone depletion (NSZD). Microbial community composition was compared to the contaminant type, concentration, and its depth of obtained soil cores. A large population of Archaea, particularly Methanomicrobia and Methanobacteria and indication of complex syntrophic relationships of methanogens, methanotrophs and bacteria were found in the contaminated cores. Different families were enriched across the LNAPL types. Results indicate methanogenic or anoxic conditions in the deeper and highly contaminated sections of the soil cores investigated. The contaminant was highly weathered, likely resulting in the formation of recalcitrant polar compounds. This research provides insight into the microorganisms fundamentally associated with LNAPL, throughout a soil depth profile above and below the water table, undergoing NSZD processes at a legacy petroleum site. It advances the potential for integration of microbial community effects on bioremediation and in response to physicochemical partitioning of LNAPL components from different petroleum types.
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23

Ye, Leiping, Andrew J. Manning, Tian-Jian Hsu, Steve Morey, Eric P. Chassignet, and Tracy A. Ippolito. "Novel Application of Laboratory Instrumentation Characterizes Mass Settling Dynamics of Oil-Mineral Aggregates (OMAs) and Oil-Mineral-Microbial Interactions." Marine Technology Society Journal 52, no. 6 (November 1, 2018): 87–90. http://dx.doi.org/10.4031/mtsj.52.6.14.

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AbstractIt is reasonable to assume that microbes played an important role in determining the eventual fate of oil spilled during the 2010 Deepwater Horizon disaster, given that microbial activities in the Gulf of Mexico are significant and diverse. However, critical gaps exist in our knowledge of how microbes influence the biodegradation and accumulation of petroleum in the water column and in marine sediments of the deep ocean and the shelf. Ultimately, this limited understanding impedes the ability to forecast the fate of future oil spills, specifically the capacity of numerical models to simulate the transport and fate of petroleum under a variety of conditions and regimes.By synthesizing recent model developments and results from field- and laboratory-based microbial studies, the Consortium for Simulation of Oil-Microbial Interactions in the Ocean (CSOMIO) investigates (a) how microbial biodegradation influences accumulation of petroleum in the water column and in marine sediments and (b) how biodegradation can be influenced by environmental conditions and impact forecasts of potential future oil spills.
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24

Atlas, Ronald M. "Fate of Petroleum Pollutants in Arctic Ecosystems." Water Science and Technology 18, no. 2 (February 1, 1986): 59–67. http://dx.doi.org/10.2166/wst.1986.0016.

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Both experimental oil release field studies, in Arctic tundra, freshwater, and marine ecosystems, and follow-up studies after Arctic and subarctic oil spillages indicate long persistence times for hydrocarbon contaminants and slow rates of microbial biodegradation. The slow rates of petroleum biodegradation in Arctic ecosystems are not due to a lack of indigenous hydrocarbon-degrading microorganisms since virtually all Arctic ecosystems contain numbers of naturally occurring populations of hydrocarbon-degrading microorganisms, and generally numbers of hydrocarbon degraders increase following addition of oil. Low temperatures alone also can not explain the limited rates of hydrocarbon biodegradation. Rather the limitation to microbial degradation of petroleum hydrocarbons in Arctic ecosystems appears to be due to the combination of several factors, including the availability of nitrogen, phosphorus, and oxygen. Although the potential for hydrocarbon degradation exists, the actual rates of hydrocarbon biodegradation in Arctic ecosystems are slow; microbial hydrocarbon degradation can decontaminate Arctic ecosystems but the time frame after a major spillage will be decades rather than years.
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25

Wang, L. Y., R. Y. Duan, J. F. Liu, S. Z. Yang, J. D. Gu, and B. Z. Mu. "Molecular analysis of the microbial community structures in water-flooding petroleum reservoirs with different temperatures." Biogeosciences 9, no. 11 (November 20, 2012): 4645–59. http://dx.doi.org/10.5194/bg-9-4645-2012.

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Abstract. Analyses of microbial communities from six water-flooding petroleum reservoirs at temperatures from 21 to 63 °C by 16S rRNA gene clone libraries indicates the presence of physiologically diverse and temperature-dependent microorganisms in these subterrestrial ecosystems. In samples originating from high-temperature petroleum reservoirs, most of the archaeal sequences belong to thermophiles affiliated with members of the genera Thermococcus, Methanothermobacter and the order Thermoplasmatales, whereas bacterial sequences predominantly belong to the phyla Firmicutes, Thermotogae and Thermodesulfobacteria. In contrast to high-temperature petroleum reservoirs, microorganisms belonging to the Proteobacteria, Methanobacteriales and Methanomicrobiales were the most encountered in samples collected from low-temperature petroleum reservoirs. Canonical correspondence analysis (CCA) revealed that temperature, mineralization, ionic type as well as volatile fatty acids showed correlation with the microbial community structures, in particular members of the Firmicutes and the genus Methanothermobacter showed positive correlation with temperature and the concentration of acetate. Overall, these data indicate the large occurrence of hydrogenotrophic methanogens in petroleum reservoirs and imply that acetate metabolism via syntrophic oxidation may represent the main methanogenic pathway in high-temperature petroleum reservoirs.
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26

Nebeská, Diana, Hana Auer Malinská, Anna Erol, Valentina Pidlisnyuk, Pavel Kuráň, Andrea Medžová, Martin Smaha, and Josef Trögl. "Stress Response of Miscanthus Plants and Soil Microbial Communities: A Case Study in Metals and Hydrocarbons Contaminated Soils." Applied Sciences 11, no. 4 (February 20, 2021): 1866. http://dx.doi.org/10.3390/app11041866.

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Second-generation biofuel crop miscanthus is one of the most promising plants tested for phytomanagement of contaminated sites. In this preliminary pot case study, the most used hybrid Miscanthus x giganteus was cultivated in three different real contaminated soils: agricultural soil contaminated with Cd; post-military soil slightly contaminated with Zn, Pb and Cd; and soil contaminated by petroleum industry with metals and hydrocarbons. The stress response of plants and soil microbial communities was monitored to receive data that are important for successful phytomanagement application. With metals only, the plant grew well, and chlorophyll fluorescence measurement proved their good vitality. Changes in leaf anatomy (leaf thickness and sclerenchyma cells area) were additionally determined in post-military soil compared to agricultural. On the contrary, in petroleum-contaminated soil, the biomass yield was too reduced and also physiological parameters were significantly decreased. The response of microbial communities also differed. In agricultural soil, no microbial stress was determined. In post-military soil, it became reduced during the experiment, and in petroleum contamination, it increased year-on-year. It could be concluded that miscanthus is suitable for cultivation in metals contaminated soils with potential for microbial communities support, but in soil contaminated by the petroleum industry, its application did not seem meaningful.
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Wang, L. Y., R. Y. Duan, J. F. Liu, S. Z. Yang, J. D. Gu, and B. Z. Mu. "Molecular analysis of the microbial community structures in water-flooding petroleum reservoirs with different temperatures." Biogeosciences Discussions 9, no. 4 (April 27, 2012): 5177–203. http://dx.doi.org/10.5194/bgd-9-5177-2012.

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Abstract. Temperature is one of the most important environmental factors regulating the activity and determining the composition of the microbial community. Analysis of microbial communities from six water-flooding petroleum reservoirs at temperatures from 20 to 63 °C by 16S rRNA gene clone libraries indicates the presence of physiologically diverse and temperature-dependent microorganisms in these subterrestrial ecosystems. In high-temperature petroleum reservoirs, most of the archaeal sequences belong to the thermophilic archaea including the genera Thermococcus, Methanothermobacter and Thermoplasmatales, most of the bacterial sequences belong to the phyla Firmicutes, Thermotogae and Thermodesulfobacteria; in low-temperature petroleum reservoirs, most of the archaeal sequences are affiliated with the genera Methanobacterium, Methanoculleus and Methanocalculus, most of the bacterial sequences to the phyla Proteobacteria, Bacteroidetes and Actinobacteria. Canonical correspondence analysis (CCA) revealed that temperature, mineralization, ionic type as well as volatile fatty acids showed correlation with the microbial community structures. These organisms may be adapted to the environmental conditions of these petroleum reservoirs over geologic time by metabolizing buried organic matter from the original deep subsurface environment and became the common inhabitants in subsurface environments.
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Li, Xingchun, Wei He, Meijin Du, Jin Zheng, Xianyuan Du, and Yu Li. "Design of a Microbial Remediation Inoculation Program for Petroleum Hydrocarbon Contaminated Sites Based on Degradation Pathways." International Journal of Environmental Research and Public Health 18, no. 16 (August 20, 2021): 8794. http://dx.doi.org/10.3390/ijerph18168794.

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This paper analyzed the degradation pathways of petroleum hydrocarbon degradation bacteria, screened the main degradation pathways, and found the petroleum hydrocarbon degradation enzymes corresponding to each step of the degradation pathway. Through the Copeland method, the best inoculation program of petroleum hydrocarbon degradation bacteria in a polluted site was selected as follows: single oxygenation path was dominated by Streptomyces avermitilis, hydroxylation path was dominated by Methylosinus trichosporium OB3b, secondary oxygenation path was dominated by Pseudomonas aeruginosa, secondary hydroxylation path was dominated by Methylococcus capsulatus, double oxygenation path was dominated by Acinetobacter baylyi ADP1, hydrolysis path was dominated by Rhodococcus erythropolis, and CoA path was dominated by Geobacter metallireducens GS-15 to repair petroleum hydrocarbon contaminated sites. The Copeland method score for this solution is 22, which is the highest among the 375 solutions designed in this paper, indicating that it has the best degradation effect. Meanwhile, we verified its effect by the Cdocker method, and the Cdocker energy of this solution is −285.811 kcal/mol, which has the highest absolute value. Among the inoculation programs of the top 13 petroleum hydrocarbon degradation bacteria, the effect of the best inoculation program of petroleum hydrocarbon degradation bacteria was 18% higher than that of the 13th group, verifying that this solution has the best overall degradation effect. The inoculation program of petroleum hydrocarbon degradation bacteria designed in this paper considered the main pathways of petroleum hydrocarbon pollutant degradation, especially highlighting the degradability of petroleum hydrocarbon intermediate degradation products, and enriching the theoretical program of microbial remediation of petroleum hydrocarbon contaminated sites.
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Abed, Raeid M. M., Nimer M. D. Safi, Jürgen Köster, Dirk de Beer, Yasser El-Nahhal, Jürgen Rullkötter, and Ferran Garcia-Pichel. "Microbial Diversity of a Heavily Polluted Microbial Mat and Its Community Changes following Degradation of Petroleum Compounds." Applied and Environmental Microbiology 68, no. 4 (April 2002): 1674–83. http://dx.doi.org/10.1128/aem.68.4.1674-1683.2002.

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ABSTRACT We studied the microbial diversity of benthic cyanobacterial mats inhabiting a heavily polluted site in a coastal stream (Wadi Gaza) and monitored the microbial community response induced by exposure to and degradation of four model petroleum compounds in the laboratory. Phormidium- and Oscillatoria-like cyanobacterial morphotypes were dominant in the field. Bacteria belonging to different groups, mainly the Cytophaga-Flavobacterium-Bacteriodes group, the γ and β subclasses of the class Proteobacteria, and the green nonsulfur bacteria, were also detected. In slurry experiments, these communities efficiently degraded phenanthrene and dibenzothiophene completely in 7 days both in the light and in the dark. n-Octadecane and pristane were degraded to 25 and 34% of their original levels, respectively, within 7 days, but there was no further degradation until 40 days. Both cyanobacterial and bacterial communities exhibited noticeable changes concomitant with degradation of the compounds. The populations enriched by exposure to petroleum compounds included a cyanobacterium affiliated phylogenetically with Halomicronema. Bacteria enriched both in the light and in the dark, but not bacteria enriched in any of the controls, belonged to the newly described Holophaga-Geothrix-Acidobacterium phylum. In addition, another bacterial population, found to be a member of green nonsulfur bacteria, was detected only in the bacteria treated in the light. All or some of the populations may play a significant role in metabolizing the petroleum compounds. We concluded that the microbial mats from Wadi Gaza are rich in microorganisms with high biodegradative potential.
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Kumari, Babita, Manvi Singh, Pankaj Kumar Srivastava, and S. N. Singh. "Degradation of Petroleum Sludge in Soil by Bacterial-Fungal Co-Culture in Presence of Organic and Inorganic Stimulants." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 5, no. 03 (July 31, 2019): 155–64. http://dx.doi.org/10.18811/ijpen.v5i03.3.

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A microcosmic study was carried out for degradation of petroleum sludge [4% (w/w) in soil] in presence of constructed microbial consortium of three bacterial strains i.e., Pseudomonas sp. BP10, Acinetobacter sp. PSM11 and Rhodococcus sp. NJ2 and two fungal strains Panicillium oxalicum PS10 and Curvularia verruculosa PS8, isolated from different petroleum hydrocarbon contaminated sites) supplemented with vermicompost and inorganic fertilizer as biostimulants. After six months of incubation, the maximum degradation of TPH from petroleum sludge was recorded as high as 80% in the presence of combination of inorganic and organic fertilizer and microbial consortium while only 33% degradation was attributed by native organisms and abiotic factors. Enhancement (%) in degradation rate of TPH due to addition of vermicompost, inorganic fertilizer and microbial consortium in separation and combination was recorded as 57%, 13%, 35% and 139%, respectively. Besides the enhancement in specific growth rate of soil microbes due to addition of nutrient, bioaugmentation of this constructed microbial consortium also boost the total bacterial and fungal strains present in petroleum sludge contaminated soil. Catabolic enzymes played an important role in degradation and maximum induction of enzymes likes catechol 1, 2 dioxygenase, catechol 2, 3 dioxygenase, catalase, laccase and dehydrogenase activity were recorded 223.89 μ mol g-1, 323.83 μ mol g-1, 0.714 μ mol H2O2 g-1, 0.623 μ mol g-1 and 3.4 μg g-1 h-1, respectively.
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31

Zhang, Z. Z., S. M. Su, Y. J. Luo, and M. Lu. "Improvement of natural microbial remediation of petroleum-polluted soil using graminaceous plants." Water Science and Technology 59, no. 5 (March 1, 2009): 1025–35. http://dx.doi.org/10.2166/wst.2009.081.

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A 150-day pot experiment was conducted with graminaceous plants grown in natural soil contaminated with petroleum. The relationships among microbial activity, dehydrogenase activity, catalase activity, soil moisture, and the petroleum degradation rate were analyzed. All three plants accelerated the degradation of petroleum compared with unplanted soil. Plant roots improved the soil moisture by about 5% (from 15% in unplanted soil to 20% in soil containing plant roots), and the number of microorganisms in the rhizosphere increased by more than three orders of magnitude. The induction of the rhizosphere environment and the intimidation of the petroleum changed the abundance and activity of the microorganisms. Dehydrogenase activity in the rhizosphere was 1.54 to 1.87 times the value in the unplanted soil, but catalase activity was 0.90 to 0.93 times the value in unplanted soil. The petroleum degradation rates in the rhizosphere were 2.33 to 3.19 times higher than in the unplanted soil. The effect of rhizosphere degradation clearly changed the hydrocarbon composition, increasing the degradation of alkane hydrocarbons with low and moderate carbon contents. The rhizosphere environment promoted degradation of the high-carbon-content hydrocarbons into low-carbon-content hydrocarbons. At the same time, the Pr/nC17, Ph/nC18, and Pr/Ph values increased by 0.99 and 2.69 units, and decreased by 1.25 units, respectively, compared with the undegraded oil. The plants also accelerated the isomerization of alkane hydrocarbons.
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32

Wang, Yonggang, Shengcai Dou, Qingfang Zhang, Abdolghaffar Ebadi, Jixiang Chen, and Mohsen Toughani. "Bacterial Separation and Community Diversity Analysis of Petroleum Contaminated Soil in Yumen Oilfield." Revista de Chimie 71, no. 3 (April 3, 2020): 595–607. http://dx.doi.org/10.37358/rc.20.3.8035.

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The problem of environmental pollution caused by the development and use of petroleum is increasingly obvious, which is a serious threat to human health. The use of microbial degradation to treat oil pollution is one of the environmentally effective, economical and practical methods.In order to explore the soil microbial diversity in the desert area of Northwest China, this paper analyzes the soil bacterial diversity of soil samples collected from different oil-contaminated areas in Yumen Oilfield for the oil pollution problem in the Yumen Oilfield in the northwest desert area, and selects the high efficiency through pure culture technology. Petroleum degradation bacteria, and research on the biological characteristics of degrading bacteria. The composition, abundance and diversity of bacterial communities in oil-contaminated soil in Yumen Oilfield were analyzed. The culturable bacteria in western oil-contaminated desert soil were separated by coating plate method. The bacterial morphology and 16S rRNA gene system development analysis were studied. The structure and diversity of bacterial community could be cultured, and the oil utilization and degradation ability of the strain could be analyzed. The microbial diversity of Yumen oil-contaminated desert soil was analyzed by Illumina Miseq high-throughput sequencing. Through research, it is found that there are abundant bacterial groups in the oil-contaminated desert soil, and there are obvious diversity. The genetic material in the variable regions of the six soil samples detected a total of 3943 0TU at 97% similarity level, and obtained the soil microbial community. Doors, 48 classes, 78 orders, 179 families and 471 genera, including most common high-efficiency petroleum-degrading bacteria. Petroleum hydrocarbon pollution can change the microbial diversity and community structure of the original soil. The size of microbial diversity in the six soil samples is B2]A1]B1]A2]C1]C2, the diversity of B2 is the highest, the diversity of C2 is the lowest, and the microbial diversity differed greatly between groups, and there was no difference in the group. Among the dominant bacteria isolated from contaminated soil, 8 strains of oil have a degradation rate of more than 30%, including the species of the genus Rhodococcus and Pseudomonas. Soil desertification in western China has a great impact on the local ecological environment. Studying the microbial diversity of desert soils and separating high-efficiency petroleum-degrading strains is of great significance for strengthening the ecological restoration of oil-contaminated environment in desert areas.
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33

Chen, Huan, Nabanita Bhattacharyya, Rui Zhang, Aixin Hou, Ryan Rodgers, and Amy M. McKenna. "Characterization of the BP petroleum residuals in the sediment of the Salt Marshes in the Northern Gulf of Mexico by Fourier Transform Ion Cyclotron Resonance mass spectrometry." International Oil Spill Conference Proceedings 2014, no. 1 (May 1, 2014): 299941. http://dx.doi.org/10.7901/2169-3358-2014-1-299941.1.

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Of the estimated 5 million barrels of crude oil released into the Gulf of Mexico from the BP Deepwater Horizon event, a fraction heavily oiled Louisiana's coastal salt marshes. Oil inputs may significantly alter the abundance, structure and diversity of the microbial communities inhabited in the sediments, and subsequently affect essential microbial services. In this study, detailed analysis was conducted to investigate the possible impact of petroleum residuals on soil microbial communities of salt marsh in northern Barataria Bay of the Gulf of Mexico after the oil spill. Sediment samples from heavily, moderately, non-oiled sites were collected after 7 months, 16 months and 29 months of the spill and Total Petroleum Hydrocarbons (TPH) were measured. Since traditional gas chromatography (GC) analysis cannot identify heavy fractions of the oil containments, we incorporated ultrahigh resolving power Fourier Transform Ion Cyclotron Resonance mass spectrometry (FT-ICR-MS) to address the compositional complexity of high molecular weight, nonvolatile petroleum fractions of the oil containments that are not readily degraded by the indigenous microbial community. The petrogentic material was extracted with methylene chloride followed by positive and negative electrospray (ESI) FT-ICR-MS characterization. These data can be correlated with the analysis of the diversity and structure of the microbial community to elucidate how the oil contamination perturbed the microbial community and how the microbes responded to the perturbation. Mass spectrometry analysis of these samples display a 1.5 to 2.5 fold increase in the molecular complexity, particularly oxygen compounds relative to the original Macondo well oil and ketone species were abundantly present in the oiled sediment extracts. The comprehensive analysis on the petroleum residues will help us better understand the fate of oil released into the environment and the long-term impact of BP oil spill.
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Shen, Tiantian, Yongrui Pi, Mutai Bao, Nana Xu, Yiming Li, and Jinren Lu. "Biodegradation of different petroleum hydrocarbons by free and immobilized microbial consortia." Environmental Science: Processes & Impacts 17, no. 12 (2015): 2022–33. http://dx.doi.org/10.1039/c5em00318k.

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35

Fedorak, P. M., J. D. Payzant, D. S. Montgomery, and D. W. Westlake. "Microbial degradation of n-alkyl tetrahydrothiophenes found in petroleum." Applied and Environmental Microbiology 54, no. 5 (1988): 1243–48. http://dx.doi.org/10.1128/aem.54.5.1243-1248.1988.

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36

Xia, Longfei. "Status of Microbial Remediation Technology in Petroleum Contaminated Land." IOP Conference Series: Earth and Environmental Science 300 (August 9, 2019): 052050. http://dx.doi.org/10.1088/1755-1315/300/5/052050.

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37

Fedorak, Phillip M., and Torren M. Peakman. "Aerobic microbial metabolism of some alkylthiophenes found in petroleum." Biodegradation 2, no. 4 (1992): 223–36. http://dx.doi.org/10.1007/bf00114554.

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38

Unimke, A. A., O. A. Mmuoegbulam, and O. C. Anika. "Microbial Degradation of Petroleum Hydrocarbons: Realities, Challenges and Prospects." Biotechnology Journal International 22, no. 2 (November 14, 2018): 1–10. http://dx.doi.org/10.9734/bji/2018/43957.

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39

Allen, Jonathan P., Estella A. Atekwana, Eliot A. Atekwana, Joseph W. Duris, D. Dale Werkema, and Silvia Rossbach. "The Microbial Community Structure in Petroleum-Contaminated Sediments Corresponds to Geophysical Signatures." Applied and Environmental Microbiology 73, no. 9 (March 9, 2007): 2860–70. http://dx.doi.org/10.1128/aem.01752-06.

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ABSTRACT The interdependence between geoelectrical signatures at underground petroleum plumes and the structures of subsurface microbial communities was investigated. For sediments contaminated with light non-aqueous-phase liquids, anomalous high conductivity values have been observed. Vertical changes in the geoelectrical properties of the sediments were concomitant with significant changes in the microbial community structures as determined by the construction and evaluation of 16S rRNA gene libraries. DNA sequencing of clones from four 16S rRNA gene libraries from different depths of a contaminated field site and two libraries from an uncontaminated background site revealed spatial heterogeneity in the microbial community structures. Correspondence analysis showed that the presence of distinct microbial populations, including the various hydrocarbon-degrading, syntrophic, sulfate-reducing, and dissimilatory-iron-reducing populations, was a contributing factor to the elevated geoelectrical measurements. Thus, through their growth and metabolic activities, microbial populations that have adapted to the use of petroleum as a carbon source can strongly influence their geophysical surroundings. Since changes in the geophysical properties of contaminated sediments parallel changes in the microbial community compositions, it is suggested that geoelectrical measurements can be a cost-efficient tool to guide microbiological sampling for microbial ecology studies during the monitoring of natural or engineered bioremediation processes.
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40

Varjani, Sunita J., and Edgard Gnansounou. "Microbial dynamics in petroleum oilfields and their relationship with physiological properties of petroleum oil reservoirs." Bioresource Technology 245 (December 2017): 1258–65. http://dx.doi.org/10.1016/j.biortech.2017.08.028.

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41

Thi Quynh Hoa, Kieu, Nguyen Vu Giang, Nguyen Thi Yen, Mai Duc Huynh, Nguyen Huu Dat, Vuong Thi Nga, Nguyen Thi Thu Ha, and Pham Thi Phuong. "Enhanced bioremediation of crude oil polluted water by a hydrocarbon-degrading Bacillus strain immobilized on polyurethane foam." Vietnam Journal of Biotechnology 18, no. 3 (November 28, 2020): 581–88. http://dx.doi.org/10.15625/1811-4989/18/3/15714.

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During the production and transportation of petroleum hydrocarbons, unsuitable operation and leakage may result in contamination of water and soil with petroleum hydrocarbons. Petroleum contamination causes significant marine environmental impacts and presents substantial hazards to human health. Bioremediation of contaminated water and soil is currently the effective and least harmful method of removing petroleum hydrocarbons from the environment. To improve the survival and retention of the bioremediation agents in the contaminated sites, microbial cells must be immobilized. It was demonstrated that immobilized microbial cells present advantages for degrading petroleum hydrocarbon pollutants compared to free suspended cells. In this study, the ability of a Bacillus strain (designed as Bacillus sp. VTVK15) to immobilize on PUF and to degrade crude oil was investigated. The immobilized Bacilllus strain had the highest number (5.38 ± 0.12 Í 108 CFU/g PUF) and a maximum attachment efficiency of 92% on PUF after 8 days. Analysis by GC-MS revealed that both free and immobilized cells of Bacillus sp. VTVK15 were able to degrade 65 and 90% of the hydrocarbons in 2% (v/v) crude oil tested after 14 days, respectively. The results suggest the potential of using PUF-immobilized Bacillus sp. VTVK15 to bioremediate petroleum hydrocarbons in an open marine environment.
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42

Shah, Raj, Richard Ashby, and Nathan Aragon. "Advancements and further research trends for microbial biosurfactants in the petroleum industry." INFORM International News on Fats, Oils, and Related Materials 32, no. 5 (May 1, 2021): 12–16. http://dx.doi.org/10.21748/inform.05.2021.12.

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Surfactants are widely used in the petroleum industry during many stages of oil recovery, refining and spill cleanup. Because these processes release surfactants directly into the environment, much research has been done on the potential for replacing the more commonly used synthetic surfactants with more eco-friendly biosurfactants.This article highlights some recent studies of the effectiveness of biosurfactants applied to various aspects of the petroleum industry.
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43

Gaylarde, Christine C., Fátima M. Bento, and Joan Kelley. "Microbial contamination of stored hydrocarbon fuels and its control." Revista de Microbiologia 30, no. 1 (1999): 01–10. http://dx.doi.org/10.1590/s0001-37141999000100001.

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The major microbial problem in the petroleum refining industry is contamination of stored products, which can lead to loss of product quality, formation of sludge and deterioration of pipework and storage tanks, both in the refinery and at the end-user. Three major classes of fuel are discussed in this article - gasoline, aviation kerosene and diesel, corresponding to increasingly heavy petroleum fractions. The fuel that presents the most serious microbiological problems is diesel. The many microorganisms that have been isolated from hydrocarbon fuel systems are listed. The conditions required for microbial growth and the methods used to monitor and to control this activity are discussed. The effects of various fuel additives, including biocides, are considered.
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44

Mohanakrishna, Gunda, Riyadh I. Al-Raoush, Ibrahim M. Abu-Reesh, and Deepak Pant. "A microbial fuel cell configured for the remediation of recalcitrant pollutants in soil environment." RSC Advances 9, no. 71 (2019): 41409–18. http://dx.doi.org/10.1039/c9ra06957g.

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45

Samim, Abdul, and Sumit Das. "PRELIMINARY PHYTOCHEMICAL SCREENING AND TO EVALUATE THE ANTI-MICROBIAL ACTIVITY OF HYDRO-ALCOHOLIC and PETROLEUM ETHER EXTRACT OF JASMINE ROOT (NYCTANTHES ARBOUR-TRISTIS). (FAMILY-NYCTAGINACEAE)." International Journal of Current Pharmaceutical Research 10, no. 4 (July 16, 2018): 47. http://dx.doi.org/10.22159/ijcpr.2018v10i4.28462.

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Objective: To estimate the anti-microbial activity of hydro-alcoholic (methanol) and petroleum ether extract of Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.Methods: The hydro-alcoholic and petroleum ether extract of the whole root part of the plant Nyctanthes arbour-tristis (family-Nyctaginaceae) was prepared and studied for phytochemical constituents by using various standard methods. The antimicrobial activity of plant extract was performed on two bacterial strains and one fungal strain using disc diffusion method.Results: The present study shows the phytochemical analysis, antimicrobial activity of the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis. Various phytochemical analyses revealed the presence of alkaloids, carbohydrates, flavonoids, tannin, phenol, terpenoids, glycosides, saponins respectively. The anti-microbial activity of the plant extract showed significant results against all three of the test organisms.Conclusion: The present study concluded that the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis (night flowering jasmine) contains the highly presence of Phytochemical constituents. The hydro-alcoholic and petroleum ether extract of the plant was found to possess promising antimicrobial activity when compared with the standards.
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46

Su, Hong, Shuofu Mi, Xiaowei Peng, and Yejun Han. "The mutual influence between corrosion and the surrounding soil microbial communities of buried petroleum pipelines." RSC Advances 9, no. 33 (2019): 18930–40. http://dx.doi.org/10.1039/c9ra03386f.

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47

Simoneit, Bernd R. T. "Hydrothermal petroleum: genesis, migration, and deposition in Guaymas Basin, Gulf of California." Canadian Journal of Earth Sciences 22, no. 12 (December 1, 1985): 1919–29. http://dx.doi.org/10.1139/e85-208.

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Hydrothermal activity and seabed mounds have been explored in Guaymas Basin by the Deep Sea Drilling Project (DSDP), piston coring, dredging, and diving with the Deep Submersible Research Vessel (DSRV) Alvin. Sedimentary organic matter, derived primarily from immature, degraded microbial detritus, is easily converted to petroleum under the hydrothermal regime. These petroleums are mature and migrate in the fluids and by diffusion to the seabed. The fluid migration is aided by near-critical aqueous solution and supercritical carbon dioxide and hydrocarbons. Petroleum compositions vary from condensates to naphthenic to waxy, all with significant amounts of asphaltenes and hydrothermal products such as olefins and toxic polynuclear aromatic hydrocarbons (PAH). The heavy ends condense at the seabed, depositing mainly as a cement with the sulfides and other minerals and to a lesser extent as entrapped oil and crystalline wax in vugs and conduits of the mounds. The PAH are high-temperature resynthesis-aromatization products from residual organic matter, and they are present in all oils but also deposit as discrete trace fractions in the hottest regions of the vent systems. Preliminary estimates of total hydrocarbon generation during hydrothermal alteration indicate that this process has a significant petroleum potential.
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48

Zhang, Fan, Yue Hui She, Sha Sha Ma, Ji Ming Hu, Ibrahim M. Banat, and Du Jie Hou. "Response of microbial community structure to microbial plugging in a mesothermic petroleum reservoir in China." Applied Microbiology and Biotechnology 88, no. 6 (August 28, 2010): 1413–22. http://dx.doi.org/10.1007/s00253-010-2841-7.

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49

Xu, Jin Lan, Jie Zhang, Ting Lin Huang, and An Long Xi. "Comparative Bioremediation of Oil Contaminated Soil by Natural Attenuation, Biostimulation and Bioaugmentation." Advanced Materials Research 777 (September 2013): 258–62. http://dx.doi.org/10.4028/www.scientific.net/amr.777.258.

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In most field studies, enhancing biodegradation of petroleum hydrocarbons depends on the specific microbial population present. It is a dispute whether inoculation microbial consortium improved the degradation of petroleum because indigenous microorganism can easily adapt to surroundings and contend for inoculation microbial consortium. Therefore, all of three technologies (natural attenuation, biostimulation and bioaugmentation) were evaluated. After 8 weeks of bioremediation, it was observed that bioaugmentation most effectively removed 53% of oil under inoculation condition. Poor oil removal of below 4% was observed under natural attenuation without inoculation. In addition, it was found that the degradation of oil in oil-polluted soil followed second-order model and acquired the dynamics equations. The half-life of natural attenuation, biostimulation and bioaugmentation was 833 days, 75days, 25days, respectively. The results indicated bioaugmentation could improve efficiently the degradation of TPH and shorten the bioremediation period.
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50

Kirkbride, K. P., Sook Miun Yap, S. Andrews, P. E. Pigou, G. Klass, A. C. Dinan, and F. L. Peddie. "Microbial Degradation of Petroleum Hydrocarbons: Implications for Arson Residue Analysis." Journal of Forensic Sciences 37, no. 6 (November 1, 1992): 13349J. http://dx.doi.org/10.1520/jfs13349j.

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