Relevant bibliographies by topics / Petroleum – Microbiology

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Petroleum – Microbiology'

Author: Grafiati
Published: 4 June 2021
Last updated: 30 July 2024

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Journal articles on the topic "Petroleum – Microbiology"

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Wackett, Lawrence P. "Petroleum microbiology." Microbial Biotechnology 5, no. 4 (June 7, 2012): 579–80. http://dx.doi.org/10.1111/j.1751-7915.2012.00350.x.

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Mu, Bo-Zhong, and Tamara N. Nazina. "Recent Advances in Petroleum Microbiology." Microorganisms 10, no. 9 (August 24, 2022): 1706. http://dx.doi.org/10.3390/microorganisms10091706.

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Van Hamme, Jonathan D., Ajay Singh, and Owen P. Ward. "Recent Advances in Petroleum Microbiology." Microbiology and Molecular Biology Reviews 67, no. 4 (December 2003): 503–49. http://dx.doi.org/10.1128/mmbr.67.4.503-549.2003.

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SUMMARY Recent advances in molecular biology have extended our understanding of the metabolic processes related to microbial transformation of petroleum hydrocarbons. The physiological responses of microorganisms to the presence of hydrocarbons, including cell surface alterations and adaptive mechanisms for uptake and efflux of these substrates, have been characterized. New molecular techniques have enhanced our ability to investigate the dynamics of microbial communities in petroleum-impacted ecosystems. By establishing conditions which maximize rates and extents of microbial growth, hydrocarbon access, and transformation, highly accelerated and bioreactor-based petroleum waste degradation processes have been implemented. Biofilters capable of removing and biodegrading volatile petroleum contaminants in air streams with short substrate-microbe contact times (<60 s) are being used effectively. Microbes are being injected into partially spent petroleum reservoirs to enhance oil recovery. However, these microbial processes have not exhibited consistent and effective performance, primarily because of our inability to control conditions in the subsurface environment. Microbes may be exploited to break stable oilfield emulsions to produce pipeline quality oil. There is interest in replacing physical oil desulfurization processes with biodesulfurization methods through promotion of selective sulfur removal without degradation of associated carbon moieties. However, since microbes require an environment containing some water, a two-phase oil-water system must be established to optimize contact between the microbes and the hydrocarbon, and such an emulsion is not easily created with viscous crude oil. This challenge may be circumvented by application of the technology to more refined gasoline and diesel substrates, where aqueous-hydrocarbon emulsions are more easily generated. Molecular approaches are being used to broaden the substrate specificity and increase the rates and extents of desulfurization. Bacterial processes are being commercialized for removal of H2S and sulfoxides from petrochemical waste streams. Microbes also have potential for use in removal of nitrogen from crude oil leading to reduced nitric oxide emissions provided that technical problems similar to those experienced in biodesulfurization can be solved. Enzymes are being exploited to produce added-value products from petroleum substrates, and bacterial biosensors are being used to analyze petroleum-contaminated environments.
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Voordouw, Gerrit. "Production-related petroleum microbiology: progress and prospects." Current Opinion in Biotechnology 22, no. 3 (June 2011): 401–5. http://dx.doi.org/10.1016/j.copbio.2010.12.005.

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Rellegadla, Sandeep, Shikha Jain, and Akhil Agrawal. "Oil reservoir simulating bioreactors: tools for understanding petroleum microbiology." Applied Microbiology and Biotechnology 104, no. 3 (December 20, 2019): 1035–53. http://dx.doi.org/10.1007/s00253-019-10311-5.

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Donets, Dmytro M., Ivan S. Suruzhiu, and Petro B. Pryima. "Analyzing the Challenges and Threats to the Petroleum Products Market in the Context of National Security." Business Inform 1, no. 552 (2024): 235–40. http://dx.doi.org/10.32983/2222-4459-2024-1-235-240.

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In this work, based on the analysis of sources of information that are in the public domain, the major threats and challenges to the petroleum products market in the context of national security are identified. The concept of national security, its connection with other types of security and the place of the market of petroleum products in these relations are considered. A model of relations between the petroleum products market in the circle of national security has been built. For the petroleum products market of Ukraine, an analysis of factors that are threats and challenges both common to all countries and specific to Ukraine is carried out. The study identifies the main threats to the petroleum products market in Ukraine, including: the possibility of losing supplies due to the lack of medium-term and long-term contracts; intersection of interests of exporting and importing countries, which is reflected in their use of specific means of solving problems; blockade of ports, destruction of existing petroleum depots; the difficulty of creating a stock of petroleum products due to the vulnerability of warehouses or petroleum depots; problems with own production of petroleum products due to the destruction or damage of existing facilities for production of petroleum products and the vulnerability of places of extraction of raw materials for petroleum products; almost complete dependence on imports; political pressure on the petroleum products market both from exporting countries and domestic political pressure. The challenges to the market include the growth of the logistics leverage, the change in the preferences of buyers from gasoline in favor of diesel fuel and the rupture of previous logistic chains with the need to create new, as well as unplanned changes in transportation due to changes in the situation at the State borders. There is also a growing demand for the use of rail and sea transport for the supply of petroleum products to the markets of Ukraine. In this regard, an additional challenge is to pursue a reasonable pricing policy in these conditions in order to prevent a sharp increase in prices in the petroleum products market.
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Wang, Ji Hua, and Shan Shan Zhang. "The Application of Microbes in Petroleum Industry." Advanced Materials Research 868 (December 2013): 542–46. http://dx.doi.org/10.4028/www.scientific.net/amr.868.542.

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With the advances in biological sciences, microbiology techniques to be applied to people in all areas of production and life, this paper introduces the microorganisms in the oil industry in all sectors such as oil and gas exploration microorganisms, microbial enhanced oil recovery and microbial degradation of the oil pollution and other aspects of the application. By summarizing the impact of microbial technology for the various aspects of oil industry, make the foundation of the microbial creative application in the field of oil industry.
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Fan, Li, Xianhe Gong, Quanwei Lv, Denghui Bin, and Li’Ao Wang. "Construction of Shale Gas Oil-Based Drilling Cuttings Degrading Bacterial Consortium and Their Degradation Characteristics." Microorganisms 12, no. 2 (February 2, 2024): 318. http://dx.doi.org/10.3390/microorganisms12020318.

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Oil-based drilling cuttings (OBDCs) contain petroleum hydrocarbons with complex compositions and high concentrations, which have highly carcinogenic, teratogenic, and mutagenic properties. In this study, three highly efficient petroleum hydrocarbon-degrading bacteria were screened from OBDCs of different shale gas wells in Chongqing, China, and identified as Rhodococcus sp. and Dietzia sp. Because of their ability to degrade hydrocarbons of various chain lengths, a new method was proposed for degrading petroleum hydrocarbons in shale gas OBDCs by combining different bacterial species. Results showed that the bacterial consortium, consisting of the three strains, exhibited the highest degradation rate for petroleum hydrocarbons, capable of degrading 74.38% of long-chain alkanes and 93.57% of short-chain alkanes, respectively. Moreover, the petroleum hydrocarbon degradation performance of the bacterial consortium in actual OBDCs could reach 90.60% in the optimal conditions, and the degradation kinetic process followed a first-order kinetic model. This study provides a certain technical reserve for the bioremediation of shale gas OBDCs.
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Robbins, Eleanora I., Mark R. Stanton, and Cheryl D. Young. "Geochemistry and Microbiology of Atacamite-Paratacamite Biofilms Floating on Underground Brine and Petroleum Pools in the White Pine Copper Mine, Michigan (USA)." Micro 3, no. 3 (August 28, 2023): 728–38. http://dx.doi.org/10.3390/micro3030051.

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At depth in an abandoned tunnel of the White Pine Copper Mine, green films of the Cu-OH-Cl minerals atacamite and paratacamite were found on standing pools of brine. Some pools were also coated with a thin layer of petroleum. Green films of atacamite were composed of individual blebs that averaged 20 μm in diameter and enclosed mixed colonies of Gram-negative, short rod-shaped, and sheathed filamentous bacteria. Carbon δ13C values in the atacamite–paratacamite mixtures reflect the isotopic values of bacteria and minor amounts of petroleum mixed with the minerals. Heterotrophic bacteria are interpreted to be using petroleum as a carbon source and may be catalyzing the precipitation of the copper hydroxy chloride minerals or acting as a template.
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Röling, Wilfred F. M., Ian M. Head, and Steve R. Larter. "The microbiology of hydrocarbon degradation in subsurface petroleum reservoirs: perspectives and prospects." Research in Microbiology 154, no. 5 (June 2003): 321–28. http://dx.doi.org/10.1016/s0923-2508(03)00086-x.

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Dissertations / Theses on the topic "Petroleum – Microbiology"

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Dyen, Michael. "Culture-dependent and independent microbial analyses of petroleum hydrocarbon contaminated Arctic soil in a mesocosm system." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=19249.

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Microbial-based strategies were investigated for eventual bioremediation of petroleum hydrocarbon-contaminated, acidic soils from Resolution Island (RI), Nunavut. A biotreatability assessment phase one study determined that supplementation of soil with commercial fertilizer and lime enhanced hydrocarbon mineralization. Phase two applied these conditions to large scale mesocosm trials, containing ~150 kg soil, incubated in a temperature cycle that represented the ambient summer conditions on RI (10 d of 1°C - 10°C for 60 d). Culture-dependent and –independent analyses of RI soil microbial communities showed the mesocosm treatment enhanced hexadecane mineralization, increased the enumerations of total microbes and viable, cold-adapted hydrocarbon-degrading microorganisms. DGGE analyses indicated emergence of a hydrocarbon-degrading community and 16S rRNA gene clone libraries showed bacterial population shift in mesocosm soils. Potentially novel isolated strains included those able to grow on hydrocarbons alone while under acidic or sub-zero conditions. This microbiological study addressed RI site conditions and presents a potential bioremediation.
Des techniques s'appuyant sur la microbiologie ont été utilisée pour évaluer la biorestauration future de sols acides, contaminés par des hydocarbures pétroliers, à Resolution Island (RI), Nunavut. Premièrement, une étude de biotraitabilité a permis de determiner que l'amendement du sol avec des fertilisants de type commercial et de la chaux améliore la dégradation des hydrocarbures. La phase deux a consisté en l'application de ces conditions à des essais de mesocosmes à grande échelle incubés à des températures représentant les conditions estivales de RI, i.e. cycle de 10 jrs (1°C-10°C) pendant 60 jrs. Des analyses de microbiologie classique et de biologie moléculaire des communatés microbiennes du sol de RI ont démontré que l'amendement des mésocosmes a permis une augmentaion de la minéralisation de l'hexadécane et un accroîssement du dénombrement de total de microorganismes ainsi que des microorganismes viables, adaptés au froid et dégradant les hydrocarbures. Des analyses par DGGE ont démontré l'apparition d'un communauté microbienne dégradant les hydrocarbures et une librairie de clones d'ARNr 16S a souligné un réarrangement des populations microbiennes présentes dans les sols de mesocosmes. Des nouvelles souches ont été isolées, incluant certaines pouvant croître sur une source unique d'hydrocarbures sous des conditions acides ou sous-zéro. Cet étude microbiologique a été faite sous des conditions respectant celles présente à RI et présente des procédés pouvant être utilisées pour la bioremediation du site.
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Belleau, Francine. "Demulsification of an industrial emulsion using microorganisms." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=66043.

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Wright, Jonathan David. "The degradation of 4-hydroxybenzoate and related aromatic compounds by Rhodotorula rubra and Penicillium citrinum isolated from diesel oil contaminated soil." Thesis, University of Hull, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.259737.

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Verde, Leandro Costa Lima 1979. "Avaliação da diversidade filogenética e funcional da microbiota envolvida na biodegradação de hidrocarbonetos em amostras de petróleo de reservatórios brasileiros = Evaluation of the phylogenetic and functional diversity of the microbiota involved in hydrocarbon biodegradation in petroleum samples from Brazilian reservoirs." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317327.

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Orientador: Valéria Maia Merzel
Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: O processo de biodegradação do petróleo em reservatórios pode resultar em mudanças na composição e propriedades físico-químicas de óleos brutos e gases naturais, as quais levam à diminuição do teor de hidrocarbonetos saturados, produzindo óleos mais pesados e com baixo valor econômico. O uso combinado de técnicas dependentes e independentes de cultivo pode nos permitir um melhor entendimento acerca da comunidade de micro-organismos que habita os reservatórios de petróleo, incluindo aqueles responsáveis por esta biodegradação. O conhecimento sobre a composição microbiana, suas funções e interações com outros micro-organismos e com o ambiente pode levar à definição de estratégias de monitoramento e/ou controle da biodegradação em reservatórios. Este estudo teve como finalidade avaliar a diversidade de micro-organismos e genes envolvidos na degradação de hidrocarbonetos presentes em amostras de petróleo provenientes de dois poços terrestres da Bacia Potiguar (RN), identificados como GMR75 (poço biodegradado) e PTS1 (poço não-biodegradado), através da construção de bibliotecas de genes catabólicos (alcano monooxigenases - alk, dioxigenases que hidroxilam anéis aromáticos ¿ ARHDs e 6-oxocyclohex-1-ene-1-carbonyl-CoA hidroxilase - bamA) e sequenciamento em larga escala de metagenoma e metatranscriptoma de enriquecimentos microbianos aeróbios. Os resultados obervados mostraram uma distribuição diferencial dos genes catabólicos entre os reservatórios, sendo o óleo biodegradado mais diverso para os genes alk e bamA. As sequências foram semelhantes aos genes alkB dos gêneros Geobacillus, Acinetobacter e Streptomyces, aos genes ARHD dos gêneros Pseudomonas e Burkholderia, e aos genes bamA do gênero Syntrophus. A análise quantitativa dos genes catabólicos de degradação de hidrocarbonetos presentes e expressos nos enriquecimentos microbianos em diferentes etapas da biodegradação do óleo, através de PCR Tempo Real, demonstrou maior atividade do gene que codifica a enzima dioxigenase nas comunidades microbianas enriquecidas, e os resultados obtidos pela técnica de microarray sugeriram a existência de novas sequências dos genes alk e ARHD provindas do reservatório de petróleo. As análises das sequências obtidas a partir do metagenoma e metatranscriptoma mostraram que a comunidade bacteriana recuperada no enriquecimento aeróbio é bastante diversa, com predominância do Filo Actinobacteria, seguido de Proteobacteria. As sequências com maior abundância e níveis de expressão foram relacionadas aos genes que codificam as proteínas ligase CoA de ácido graxo de cadeia longa, envolvida na degradação de compostos aromáticos; descarboxilase, envolvida com o ciclo do glioxilato, e o fator sigma da RNA polimerase, envolvida com a regulação da resposta ao estresse oxidativo, sugerindo uma adaptação da comunidade microbiana às condições do enriquecimento e um processo inicial de biodegradação dos hidrocarbonetos. Os resultados obtidos neste trabalho fornecem dados inéditos sobre a diversidade de genes catabólicos e de membros da comunidade microbiana potencialmente envolvidos com a degradação do óleo em reservatórios de petróleo
Abstract: The process of oil biodegradation in reservoirs may result in changes in the composition and physico-chemical properties of crude oils and natural gases, which lead to the decrease of the content of saturated hydrocarbons, producing heavy oils and with low economic value. The combined use of both dependent and independet cultivation techniques may allow us to better understand the microbial community inhabiting oil reservoirs, including those microorganisms responsible for oil degradation. The knowledge about the microorganisms, ther functions and interactions with other microorganisms and the environment may lead to the definition of monitoring and/or control strategies of biodegradation in oil reservoirs. This study aimed at evaluating the diversity of microorganisms and genes involved in the degradation of hydrocarbons present in oil samples from two onshore reservoirs at Potiguar Basin (RN), identified as GMR75 (biodegraded) and PTS1 (non- biodegraded), through the construction of catabolic gene libraries (alkane monooxygenases - alk, aromatic ring hydroxylating dioxygenases ¿ ARHD and 6-oxocyclohex-1-ene-1-carbonyl-CoA hydroxylase - bamA) and highthroughput sequencing of metagenome and metatranscriptome from aerobic microbial enrichments. Results observed showed a differential distribution of catabolic genes between the reservoirs, being the biodegraded oil more diverse for the alk and bamA genes. The sequences were similar to alkB genes from Geobacillus, Acinetobacter and Streptomyces genera, to the ARHD genes from Pseudomonas and Burkholderia genera, and to the bamA genes from Syntrophus genus. Quantitative analysis of the hydrocarbon degradation genes present and expressed in the microbial enrichments during the different phases of oil biodegradation by Real-Time PCR showed that there was a higher activity of dioxygenase enzymes in the enriched microbial communities and results from microarray assays suggested the existence of new alk and ARHD gene sequences originated from the oil reservoir. Metagenomic and metatranscriptomic analyses showed a highly diverse bacterial community, dominated by the Phylum Actinobacteria, followed by Proteobacteria. The most abundant and active sequences were affiliated to the Long-chain-fatty-acid-CoA ligase protein, involved in the degradation of aromatic compounds; decarboxylase, which is involved with the glyoxylate cycle, and RNA polymerase sigma factor, which is involved in regulating the oxidative stress response, suggesting an adaptation of the microbial community to the enrichment conditions and an initial process of biodegradation of hydrocarbon compounds. The results obtained in this work bring innovative data on the diversity of catabolic genes and microbial community members potentially involved with oil degradation in petroleum reservoirs
Doutorado
Genetica de Microorganismos
Doutor em Genetica e Biologia Molecular
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Uzukwu, Chukwuemeka. "The biodegradation of hydrocarbons using open mixed culture for microbial enhanced oil recovery and bioremediation." Thesis, University of Aberdeen, 2017. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=231857.

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This research has investigated the biodegradation of hydrocarbons particularly n-alkanes using open mixed culture which is relevant for both microbial enhanced oil recovery (MEOR) and the bioremediation of hydrocarbon contaminated soils. Biodegradation of n-C12, C14, C16, C18, C20 and some readily biodegradable substrates (glucose, acetic acid and ethanol) was studied using a respirometric method developed to assess the biodegradability of these compounds. Laboratory batch and semi-continuous experiments were performed in small-scale bioreactors at room temperature and 40oC under various conditions i.e. aerobic, anoxic with nitrate, sulfate reducing and completely anaerobic conditions using two different sources of open mixed microbial cultures obtained from an agricultural site and anaerobic digestion plant. Glucose, acetic acid, ethanol, C12, C14 and C16 were degraded microbially under aerobic batch conditions to nondetectable levels at room temperature and 40oC using the two sources of inoculum whereas C18 and C20 were degraded partially under room temperature and to nondetectable levels at 40oC with the two inocula sources. Under aerobic semi-continuous, glucose and the n-alkane substrate were biodegraded even at low hydraulic retention time (HRT). Under anaerobic conditions, the n-alkanes were utilized by the soil inoculum at room temperature and at 40oC with nitrate as the electron acceptor but no microbial activity was observed under sulfate reducing and completely anaerobic conditions. The open mixed cultures require an initial acclimation period before utilizing the substrates. The acclimation period was significantly shorter under aerobic conditions than anaerobic conditions for the n-alkanes. Acclimation periods of approximately 1-2 days under aerobic conditions was observed for the readily biodegradable substrates and 2 days for glucose under anoxic conditions. The acclimation periods for the nalkanes was between 3-5 days under aerobic conditions and approximately 2 weeks under anoxic conditions. The acclimation period was not affected by the substrate concentration and inoculum type however, for the n-alkanes, the acclimation period was reduced by 1-2days under aerobic conditions at 40oC. The biodegradation of the liquid hydrocarbons was more significant than the solids at room temperature but in general higher temperature increased the degree of biodegradation. The electron acceptor consumption data i.e. dissolved oxygen and nitrate consumption data obtained was mathematically modelled using Monod kinetics to obtain biokinetic parameters. Good fittings between the model solution and the experimental data was obtained. The biokinetic parameters obtained were within the range of values reported in literature. The use of respirometric data for the estimation of biodegradation kinetic parameters can be very reliable. The consistency of the data obtained show that the approach is very reproducible and quality information can be obtained. The results of this study showed that the open mixed microbial cultures from soil and AD inocula contained diverse microorganisms capable of utilizing both liquid and solid n-alkanes at room temperature and 40oC under aerobic and anoxic conditions.
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Nevárez-Moorillón, Guadalupe Virginia. "Biodegradation of Certain Petroleum Product Contaminants in Soil and Water By Selected Bacteria." Thesis, University of North Texas, 1995. https://digital.library.unt.edu/ark:/67531/metadc332474/.

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Soil contamination by gasoline underground storage tanks is a critical environmental problem. The results herein show that in situ bioremediation using indigenous soil microorganisms is the method of choice. Five sites were selected for bioremediation based on the levels of benzene, toluene, ethylbenzene and xylene and the amount of total petroleum hydrocarbons in the soil. Bacteria capable of degrading these contaminants were selected from the contaminated sites and grown in 1,200 I mass cultures. These were added to the soil together with nutrients, water and air via PVC pipes.
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Jones, Cleveland Maximino. "Avaliação do possível impacto das técnicas de MEOR (Microbial Enhanced Oil Recovery) no fator de recuperação das reservas de petróleo e gás do Brasil." Universidade do Estado do Rio de Janeiro, 2014. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=7103.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Os métodos tradicionais de estimular a produção de petróleo, envolvendo a injeção de água, vapor, gás ou outros produtos, estabeleceram a base conceitual para novos métodos de extração de óleo, utilizando micro-organismos e processos biológicos. As tecnologias que empregam os processos de bioestimulação e bioaumentação já são amplamente utilizadas em inúmeras aplicações industriais, farmacêuticas e agroindustriais, e mais recentemente, na indústria do petróleo. Dada a enorme dimensão econômica da indústria do petróleo, qualquer tecnologia que possa aumentar a produção ou o fator de recuperação de um campo petrolífero gera a expectativa de grandes benefícios técnicos, econômicos e estratégicos. Buscando avaliar o possível impacto de MEOR (microbial enhanced oil recovery) no fator de recuperação das reservas de óleo e gás no Brasil, e quais técnicas poderiam ser mais indicadas, foi feito um amplo estudo dessas técnicas e de diversos aspectos da geologia no Brasil. Também foram realizados estudos preliminares de uma técnica de MEOR (bioacidificação) com possível aplicabilidade em reservatórios brasileiros. Os resultados demonstram que as técnicas de MEOR podem ser eficazes na produção, solubilização, emulsificação ou transformação de diversos compostos, e que podem promover outros efeitos físicos no óleo ou na matriz da rocha reservatório. Também foram identificadas bacias petrolíferas brasileiras e recursos não convencionais com maior potencial para utilização de determinadas técnicas de MEOR. Finalmente, foram identificadas algumas técnicas de MEOR que merecem maiores estudos, entre as técnicas mais consolidadas (como a produção de biossurfatantes e biopolímeros, e o controle da biocorrosão), e as que ainda não foram completamente viabilizadas (como a gaseificação de carvão, óleo e matéria orgânica; a dissociação microbiana de hidratos de gás; a bioconversão de CO2 em metano; e a bioacidificação). Apesar de seu potencial ainda não ser amplamente reconhecido, as técnicas de MEOR representam o limiar de uma nova era na estimulação da produção de recursos petrolíferos existentes, e até mesmo para os planos de desenvolvimento de novas áreas petrolíferas e recursos energéticos. Este trabalho fornece o embasamento técnico para sugerir novas iniciativas, reconhecer o potencial estratégico de MEOR, e para ajudar a realizar seu pleno potencial e seus benefícios.
The traditional methods of stimulating production, involving the injection of water, steam, gas or other products, have established the conceptual basis for new methods of oil extraction, utilizing microorganisms and biological processes. Technologies that employ biostimulation and bioaugmentation processes are widely utilized in numerous industrial, pharmaceutical and agroindustrial applications, and, more recently, in the oil industry. Given the enormous economic dimension of the oil industry, any technology that can increase production or recovery of an oil field creates the expectation of large technical, economic and strategic benefits. In order to assess the possible impact of MEOR (Microbial Enhanced Oil Recovery) on the recovery factor of oil and gas reserves in Brazil, and which techniques might be most indicated, a wide ranging study of those techniques and of various aspects of the geology of Brazil was carried out. Preliminary studies of a MEOR technique (bioacidification) with possible application in Brazilian reservoirs were also carried out. The results demonstrate that MEOR techniques can be effective in the production, solubilization, emulsification or transformation of several compounds, and that they can promote other physical effects in the oil or the reservoir rock matrix. Brazilian oil basins and unconventional resources with potential for utilization of certain MEOR techniques were also identified. Finally, certain MEOR techniques that deserve further studies were identified, involving both more consolidated techniques (such as biosurfactant and biopolymer production, and the control of microbially induced corrosion), as well as those that have not yet fully proven their viability (such as coal, oil and organic matter gasification; microbial dissociation of gas hydrates; bioconversion of CO2 into methane; and bioacidification). Despite the fact that their potential is not yet fully recognized, MEOR techniques represent the dawn of a new era in the stimulation of production of existing oil resources, and even in the production development plans of new oil and other energy resources. This work furnishes the technical basis for suggesting new initiatives, for recognizing the strategic potential of MEOR, and for helping to realize the full potential of MEOR and its benefits.
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Silva, Tiago Rodrigues e. "Caracterização polifásica da microbiota presente em amostras de petróleo de reservatórios brasileiros." [s.n.], 2010. http://repositorio.unicamp.br/jspui/handle/REPOSIP/317328.

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Orientador: Valéria Maia Merzel
Dissertação (mestrado) - Universidade Estadual de Campinas, Insituto de Biologia
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Resumo: Estudos realizados em reservatórios de petróleo têm evidenciado que parte da microbiota associada a este tipo de ambiente é representada por bactérias e arqueias de distribuição geográfica bastante ampla e que diversos destes organismos têm potencial para transformar compostos orgânicos e inorgânicos, atuando na interface óleo-água dos reservatórios. A investigação de micro-organismos com potencial para biodeterioração, biodegradação e biocorrosão encontrados em depósitos petrolíferos é de grande importância, uma vez que estes organismos podem estar relacionados com a perda da qualidade do petróleo nos reservatórios e etapas subseqüentes de exploração. Este estudo teve como finalidade comparar a microbiota presente em amostras de óleo de dois poços de petróleo terrestres da Bacia Potiguar (RN), identificados como GMR75 (poço biodegradado) e PTS1 (poço não-biodegradado). As comunidades microbianas foram estudadas usando técnicas de cultivo (enriquecimentos microbianos e isolamento) e independentes de cultivo (construção de bibliotecas de genes RNAr 16S). Os micro-organismos cultivados de ambos os poços mostraram-se afiliados aos filos Actinobacteria, Firmicutes e Proteobacteria. As bibliotecas de gene RNAr 16S foram construídas a partir de DNA total extraído do petróleo bruto. Ambas as bibliotecas de bactérias revelaram uma grande diversidade, com 8 filos diferentes para o poço GMR75, Actinobacteria, Bacteroidetes, Deferribacteres, Spirochaetes, Firmicutes, Proteobacteria, Thermotoga e Synergistetes, e 5 filos para o poço PTS1, Actinobacteria, Chloroflexi, Firmicutes, Proteobacteria e Thermotogae. A biblioteca de genes RNAr 16S de arqueias só foi obtida para o poço GMR75 e todos os clones encontrados mostraram-se relacionados a membros da ordem Methanobacteriales. Os resultados de diversidade sugerem que a metanogênese é o processo terminal dominante no poço, o que indica uma biodegradação anaeróbia. A comparação dos estudos dependente e independente de cultivo mostrou que alguns gêneros, como Janibacter, Georgenia, Saccharopolyspora, Tessaracoccus, Brevundimonas e Brachymonas não foram encontradas na abordagem independente de cultivo, sugerindo que mais clones devam ser seqüenciados para cobrir toda a diversidade presente na amostra. Nossa hipótese de que poderia haver algum agente antimicrobiano inibindo o crescimento de bactérias degradadoras de hidrocarbonetos no poço não-biodegradado não foi confirmada. No entanto, durante os testes realizados, uma bactéria, Bacillus pumilus, isolada em estudos anteriores de reservatórios da Bacia de Campos, apresentou resultados positivos de inibição para todas as linhagens testadas como indicadoras, e os testes de caracterização do composto revelaram ser este um diterpeno da classe das Ciatinas.
Abstract: Recent studies from oil fields have shown that microbial diversity is represented by bacteria and archaea of wide distribution, and that many of these organisms have potential to metabolize organic and inorganic compounds. The potential of biodeterioration, biodegradation and biocorrosion by microorganisms in oil industry is of great relevance, since these organisms may be related with the loss of petroleum quality and further exploration steps. The aim of the present study was to compare the microbial communities present in two samples from terrestrial oil fields from Potiguar basin (RN - Brazil), identified as GMR75 (biodegraded oil) and PTS1 (non-biodegraded oil). Microbial communities were investigated using cultivation (microbial enrichments and isolation) and molecular approaches (16S rRNA gene clone libraries). The cultivated microorganisms recovered from both oil-fields were affiliated with the phyla Actinobacteria, Firmicutes and Proteobacteria. The 16S rRNA gene clone libraries were constructed from metagenomic DNA obtained from crudeoil. Both bacterial libraries revealed a great diversity, encompassing representatives of 8 different phyla for GMR75, Actinobacteria, Bacteroidetes, Deferribacteres, Spirochaetes, Firmicutes, Proteobacteria, Thermotogae and Synergistetes, and of 5 different phyla, Actinobacteria, Chloroflexi, Firmicutes, Proteobacteria and Thermotoga, for PTS1. The archaeal 16S rRNA clone library was obtained only for GMR75 oil and all phylotypes were affiliated with order Methanobacteriales. Diversity resuts suggest that methanogenesis is the dominant terminal process in GMR75 reservoir, driven by anaerobic biodegradation. The cross-evaluation of culture-dependent and independent techniques indicates that some bacterial genera, such as Janibacter, Georgenia, Saccharopolyspora, Tessaracoccus, Brevundimonas and Brachymonas, were not found using the the 16S rRNA clone library approach, suggesting that additional clones should be sequenced in order to cover diversity present in the sample. Our hypothesis that biodegrading bacterial populations could be inhibited by antimicrobialproducing microorganisms in the non biodegraded oil field (PTS1) was not confirmed. However, one Bacillus pumilus strain, previously isolated from Campos Basin reservoirs, showed positive results in inhibitory tests for all indicator strains. Chemical analyses allowed us to identify the compound as a diterpen from the Cyathin class.
Mestrado
Genetica de Microorganismos
Mestre em Genética e Biologia Molecular
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Moliva, Juan Ignacio. "The Lung Mucosa and its Impact on Mycobacterium tuberculosis Pathogenesis and Bacillus Calmette-Guerin Vaccine Efficacy." The Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1497602977755499.

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Oliveira, Carla Gabriela Braga de. "Utilização de fontes oleofílicas de nitrogênio e fósforo para biorremediação de petróleo em areia de praia." Universidade Federal de Viçosa, 2011. http://locus.ufv.br/handle/123456789/5346.

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Degradation of petroleum hydrocarbons by micro-organisms in coastal environments is a very interesting strategy for remediation of these compounds. However, microbial degradation is often limited by nutrients avaiability, particularly nitrogen and phosphorus. In this work, we compared the effect of addition of water-soluble (urea and KH2PO4) or oleophilic (urea-formaldehyde resin and soy lecithin) nitrogen and phosphorus sources on oil degradation by indigenous micro-organisms and bacteria in consortia in microcosms composed by beach sand contaminated with crude oil. The microcosms were submitted to daily water exchanges representing tides activities. We also analysed the retention capability of both nutrient sources in contaminated sediment. For this, we performed UFC.mL-1 counts and quantification of nitrogen and phosphorus concentrations in the water during the experiment. The remaining oil in the sand was extracted with hexane and quantified by spectrophotometry, in which the degradation corresponds to the difference between the initial and final amounts of oil. Water-soluble nutrients have been shown to be gradually washed away during the water exchange and not remained in the sediment for bacterial supply. However, an opposite pattern was verified for oleophilic nutrients, which is a desirable feature for application in environments with intense action of waves and tides. Despite the increased retention of oleophilic nutrients, there were no significant differences in microbial counts between both treatments. In addition, oil degradation occurred mainly in those microcosms supplied with water-soluble nutrients. Although the inoculated micro-organisms are able to use the oleophilic nutrient sources, more detailed studies on the concentration of nutrients to be applied and the availability of nutrients for micro-organisms are necessary in order to maximize the stimulus generated.
A degradação de hidrocarbonetos de petróleo por micro-organismos em ambientes costeiros é uma estratégia extremamente interessante de remediação desses compostos enquanto contaminantes ambientais. No entanto, essa biodegradação é frequentemente limitada pela disponibilidade de nutrientes, principalmente nitrogênio e fósforo. Neste trabalho foi comparado o efeito da adição de fontes de nitrogênio e fósforo solúveis em água (uréia e KH2PO4) e oleofílicas (resina uréia-formaldeído e lecitina de soja) sobre a degradação de petróleo cru, em microcosmos de areia de praia, por micro-organismos nativos ou adicionados em consórcios. As fontes de nutrientes foram ainda comparadas quanto a sua retenção no sedimento contaminado após trocas diárias de água. Para isso, realizou-se contagens de UFC.mL-1 e análises da concentração de nitrogênio e de fósforo na água. O óleo remanescente na areia foi extraído com hexano e quantificado por espectrofotometria, sendo a degradação correspondente a diferença entre as quantidades inicial e final de petróleo. Verificou-se que os nutrientes hidrossolúveis não permaneceram no sedimento, mas foram gradativamente lavados pela remoção diária de água. Um comportamento inverso ocorreu para os nutrientes oleofílicos, sendo essa uma característica desejável para sua aplicação em ambientes com intensa ação de ondas e marés. Apesar da maior retenção de nutrientes quando esses são oleofílicos, não se verificou diferenças consideráveis nas contagens microbianas entre os dois tratamentos. Além disso, a degradação do óleo ocorreu principalmente naqueles microcosmos supridos com os nutrientes solúveis em água. Embora os micro-organismos inoculados sejam capazes de utilizar as fontes oleofílicas como nutrientes, estudos mais detalhados da concentração a ser aplicada e da disponibilização desses nutrientes para os micro-organismos são necessários, a fim de se maximizar o estímulo gerado.
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Books on the topic "Petroleum – Microbiology"

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Ollivier, Bernard, and Michel Magot, eds. Petroleum Microbiology. Washington, DC, USA: ASM Press, 2005. http://dx.doi.org/10.1128/9781555817589.

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An Stepec, Biwen Annie, Kenneth Wunch, and Torben Lund Skovhus. Petroleum Microbiology. Boca Raton: CRC Press, 2024. http://dx.doi.org/10.1201/9781003287056.

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Timmis, K. N. Handbook of hydrocarbon and lipid microbiology. Berlin: Springer, 2010.

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Rafael, Vazquez-Duhalt, and Quintero Ramírez Rodolfo 1947-, eds. Petroleum biotechnology: Developments and perspectives. Amsterdam: Elsevier, 2004.

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S, Blenkinsopp, and Alaska. Dept. of Environmental Conservation., eds. Assessment of the freshwater biodegradation potential of oils commonly transported in Alaska: Final report. [Edmonton, AB, Canada]: Environment Canada, Emergencies Science Division, 1996.

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International Symposium on Microbiology of the Deep Subsurface (1st 1990 Orlando, Florida). Proceedings of the first International symposium on microbiology of the deep subsurface. Aiken, S. C: Westinghouse Savannah River Company, 1990.

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R, Engelhardt F., ed. Petroleum effects in the arctic environment. London: Elsevier Applied Science Publishers, 1985.

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Britain), Energy Institute (Great, ed. Guidelines for the investigation of the microbial content of petroleum fuels and for the implementation of avoidance and remedial strategies. 2nd ed. London: Energy Institute, 2008.

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Britain), Energy Institute (Great, ed. Guidelines for the investigation of the microbial content of petroleum fuels and for the implementation of avoidance and remedial strategies. 2nd ed. London: Energy Institute, 2008.

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Vandecasteele, Jean-Paul. Microbiologie pétrolière: Concepts, implications environnementales, applications industrielles. Paris: Technip, 2005.

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Book chapters on the topic "Petroleum – Microbiology"

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Singh, Ajay, Jonathan D. Van Hamme, Ramesh C. Kuhad, Nagina Parmar, and Owen P. Ward. "Subsurface Petroleum Microbiology." In Geomicrobiology and Biogeochemistry, 153–73. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-41837-2_9.

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An Stepec, Biwen Annie, Kenneth Wunch, Torben Lund Skovhus, Julia R. de Rezende, Markus Pichler, Susmitha Purnima Kotu, Sarah E. Gasda, and Nicole Dopffel. "Petroleum Microbiology's Metamorphosis." In Petroleum Microbiology, 3–17. Boca Raton: CRC Press, 2024. http://dx.doi.org/10.1201/9781003287056-2.

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El-Gendy, Nour Shafik, Hussein Nabil Nassar, and James G. Speight. "Petroleum Microbiology and Nanotechnology." In Petroleum Nanobiotechnology, 1–67. Boca Raton: Apple Academic Press, 2021. http://dx.doi.org/10.1201/9781003160564-1.

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Planckaert, Marie. "Oil Reservoirs and Oil Production." In Petroleum Microbiology, 1–19. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817589.ch1.

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Sunde, Egil, and Terje Torsvik. "Microbial Control of Hydrogen Sulfide Production in Oil Reservoirs." In Petroleum Microbiology, 199–213. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817589.ch10.

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McInerney, Michael J., David P. Nagle, and Roy M. Knapp. "Microbially Enhanced Oil Recovery: Past, Present, and Future." In Petroleum Microbiology, 215–37. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817589.ch11.

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Kilbane, John J. "Biotechnological Upgrading of Petroleum." In Petroleum Microbiology, 239–55. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817589.ch12.

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van Beilen, Jan B., and Berhard Witholt. "Diversity, Function, and Biocatalytic Applications of Alkane Oxygenases." In Petroleum Microbiology, 257–75. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817589.ch13.

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Rabus, Ralf. "Biodegradation of Hydrocarbons Under Anoxic Conditions." In Petroleum Microbiology, 277–99. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817589.ch14.

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Fayolle, Françoise, and Frédéric Monot. "Biodegradation of Fuel Ethers." In Petroleum Microbiology, 301–16. Washington, DC, USA: ASM Press, 2014. http://dx.doi.org/10.1128/9781555817589.ch15.

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Conference papers on the topic "Petroleum – Microbiology"

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Horacek, G. L. "The Oilfield Microbiology Assistance System (OMAS)." In Petroleum Computer Conference. Society of Petroleum Engineers, 1988. http://dx.doi.org/10.2118/17783-ms.

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Poulsen, Morten, Peter Frank Sanders, Uffe Sognstrup Thomsen, and Thomas Lundgaard. "Use of Advanced Molecular Microbiology Methods to Manage Microbial Corrosion Issues in Topside Facilities." In Abu Dhabi International Petroleum Exhibition & Conference. Society of Petroleum Engineers, 2016. http://dx.doi.org/10.2118/183526-ms.

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Sengupta, Debanjan. "Application of Biotechnology in Petroleum Industry - Microbial Enhanced Oil Recovery." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0088.

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Bauer, J. F., M. M. Amro, T. Nassan, and H. Alkan. "Reservoir Engineering Aspects of Geologic Hydrogen Storage." In International Petroleum Technology Conference. IPTC, 2024. http://dx.doi.org/10.2523/iptc-23943-ms.

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Abstract Safe and effective large-scale storage of hydrogen (H2) is one of the biggest challenges of the global energy transition. The only way to realize this is storage in geological formations. The aim of this study is to address and discuss the reservoir engineering (RE) aspects of geological H2 storage (GHS). The study is based on two sources: first, a comprehensive literature review, and second, experimental and numerical work performed by our institute. The current state of the art regarding the principles of reservoir engineering on the application of GHS is reviewed and summarized. Atypical properties of H2, with its lower density, viscosity and compressibility factor higher than one, increase uncertainties in the definition of capacity, injectivity, and confinement. In addition, the abiotic and biotic reactivity of H2 should be considered in the associated changes in petrophysical properties and molecular mass transfer in subsurface storage formations. Therefore, both geochemistry and reservoir microbiology are inseparable components of reservoir engineering of GHS. The sealing of H2 storage in a porous reservoir with caprock is due to the interplay between potentially higher capillary threshold pressure but higher diffusivity of H2, while the technically impermeable assumption of most deep salt formations can be considered as valid for H2 storage in caverns. Such changes can also affect the injectivity of H2 through plugging or dissolution. Well integrity is of particular concern when abandoned-old gas wells are reused. Especially at higher temperatures, hydrogen can behave more actively to support metal oxidation processes at the casing-cement contact and microbiological activity can promote these reactions. In addition, the permeability of the hardened cement samples to H2 is highly dependent on the effective pressure. An overview of the reservoir engineering aspects of GHS is compiled from recent publications. We integrate key findings with our experimental results to provide essential guidance for front-end engineering and challenges to be addressed in future work. Monitoring of the reservoir pressure, as an indicator of microbial activity, is of great importance. Therefore, measures to control microbial activity have to be drawn, taking into account the site-specific characteristics.
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