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Relevant bibliographies by topics / PGREEN plasmid

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Academic literature on the topic 'PGREEN plasmid'

Author: Grafiati

Published: 30 May 2022

Last updated: 31 July 2025

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Journal articles on the topic "PGREEN plasmid"

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Cao, Thi Thu Thuy, Thi Bich Ngoc Nguyen, Duc Quan Nguyen, Tat Thanh Le, Huy Hoang Nguyen, and Thi Thu Hue Huynh. "Producing a recombinant vector that carries the vascular class III peroxidase (\(\textit{PRX1}\)) gene from \(\textit{Catharanthus roseus}\)." Academia Journal of Biology 45, no. 4 (2023): 53–62. http://dx.doi.org/10.15625/2615-9023/18664.

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Terpenoid indole alkaloids (TIAs) vincristine and vinblastine are two essential anticancer drugs used in cancer treatments. These compounds are naturally available only in Catharanthus roseus in low accumulation, leading to efforts to upregulate TIAs substances through other expression organisms. The combination of vindoline and catharanthine into α-3∼,4∼-anhydrovinblastine (AVLB) is an important step for the biosynthesis of vinca alkaloids, which serves as a precursor to vinblastine and vincristine. Vascular class III peroxidase (PRX1) plays a crucial role in catalyzing the dimerization react

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Tomas, Christopher A., Neil E. Welker, and Eleftherios T. Papoutsakis. "Overexpression of groESL in Clostridium acetobutylicum Results in Increased Solvent Production and Tolerance, Prolonged Metabolism, and Changes in the Cell's Transcriptional Program." Applied and Environmental Microbiology 69, no. 8 (2003): 4951–65. http://dx.doi.org/10.1128/aem.69.8.4951-4965.2003.

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ABSTRACT DNA array and Western analyses were used to examine the effects of groESL overexpression and host-plasmid interactions on solvent production in Clostridium acetobutylicum ATCC 824. Strain 824(pGROE1) was created to overexpress the groESL operon genes from a clostridial thiolase promoter. The growth of 824(pGROE1) was inhibited up to 85% less by a butanol challenge than that of the control strain, 824(pSOS95del). Overexpression of groESL resulted in increased final solvent titers 40% and 33% higher than those of the wild type and plasmid control strains, respectively. Active metabolism

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Tomas, Christopher A., Jeffrey Beamish, and Eleftherios T. Papoutsakis. "Transcriptional Analysis of Butanol Stress and Tolerance in Clostridium acetobutylicum." Journal of Bacteriology 186, no. 7 (2004): 2006–18. http://dx.doi.org/10.1128/jb.186.7.2006-2018.2004.

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ABSTRACT The effects of challenges with low (0.25%, vol/vol) and high (0.75%) concentrations of butanol on the growth, glucose metabolism, product formation, and transcriptional program of the solvent-tolerant Clostridium acetobutylicum strain 824(pGROE1) and the plasmid control strain 824(pSOS95del) were used to study solvent tolerance and stress response. Strain 824(pGROE1) was generated by groESL overexpression. The growth of 824(pGROE1) was less inhibited than that of 824(pSOS95del), and 824(pGROE1) was able to metabolize glucose over the entire course of the culture (60 h postchallenge) w

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Yu, Tongya, Yingying Lin, Yuzhen Xu, et al. "Repressor Element 1 Silencing Transcription Factor (REST) Governs Microglia-Like BV2 Cell Migration via Progranulin (PGRN)." Neural Plasticity 2020 (November 25, 2020): 1–9. http://dx.doi.org/10.1155/2020/8855822.

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Microglia activation contributes to Alzheimer’s disease (AD) etiology, and microglia migration is a fundamental function during microglia activation. The repressor element-1 silencing transcription factor (REST), a powerful transcriptional factor, was found to play a neuroprotective role in AD. Despite its possible role in disease progression, little is known about whether REST participates in microglia migration. In this study, we aimed to explore the function of REST and its molecular basis during microglia migration under Aβ1-42-treated pathological conditions. When treated by Aβ1-42 REST w

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Kudryakova, Irina, Alexey Afoshin, Elena Leontyevskaya та Natalia Leontyevskaya (Vasilyeva). "The First Homologous Expression System for the β-Lytic Protease of Lysobacter capsici VKM B-2533T, a Promising Antimicrobial Agent". International Journal of Molecular Sciences 23, № 10 (2022): 5722. http://dx.doi.org/10.3390/ijms23105722.

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A successful homologous expression system based on Lysobacter capsici VKM B-2533T and the plasmid pBBR1-MCS5 was first developed for a promising bacteriolytic enzyme of this bacterium, β-lytic protease (Blp). In the expression strains, blp gene expression under the regulation of the GroEL(A) and T5 promoters increased by 247- and 667-fold, respectively, as compared with the wild-type strain. After the cultivation of the expression strains L. capsici PGroEL(A)-blp and L. capsici PT5-blp, the Blp yield increased by 6.7- and 8.5-fold, respectively, with respect to the wild-type strain. The cultiv

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Wang, Lili, Qiandong Hou, and Guang Qiao. "Genome-Wide Identification and Expression Analysis of the Sweet Cherry Whirly Gene Family." Current Issues in Molecular Biology 46, no. 8 (2024): 8015–30. http://dx.doi.org/10.3390/cimb46080474.

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Sweet cherry (Prunus avium) is one of the economically valuable horticultural fruit trees and it is widely cultivated throughout the world. Whirly (WHY) genes are a unique gene family with few members and have important biological functions in plant growth, development, and response to abiotic stress. This study utilized whole-genome identification to conduct a comprehensive analysis of the WHY genes in sweet cherry and examined their transcription levels in different tissues and under abiotic stress to explore their functions. Two WHY genes were identified in the sweet cherry genome and named

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Jääskeläinen, J., A. Deeb, J. W. Schwabe, N. P. Mongan, H. Martin, and I. A. Hughes. "Human androgen receptor gene ligand-binding-domain mutations leading to disrupted interaction between the N- and C-terminal domains." Journal of Molecular Endocrinology 36, no. 2 (2006): 361–68. http://dx.doi.org/10.1677/jme.1.01885.

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Most mutations in the androgen receptor (AR) ligand-binding domain (LBD) disrupt binding of the natural ligands: dihydrotestosterone and testosterone. Some AR LBD mutations do not affect ligand binding but they disrupt androgen-induced interaction of the N-terminal motif FXXLF and C-terminal activation function 2 (AF2). As N-/C-terminal interaction requires binding of agonists that have androgen activity in vivo, it correlates well with the phenotype. To study this further, we searched the Cambridge intersex database for patients with a detected missense mutation in the AR LBD presenting with

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Zoheir, Ahmed E., Morgan S. Sobol, Laura Meisch, et al. "A three-colour stress biosensor reveals multimodal response in single cells and spatiotemporal dynamics of biofilms." npj Biofilms and Microbiomes 9, no. 1 (2023). http://dx.doi.org/10.1038/s41522-023-00424-1.

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AbstractThe plethora of stress factors that can damage microbial cells has evolved sophisticated stress response mechanisms. While existing bioreporters can monitor individual responses, sensors for detecting multimodal stress responses in living microorganisms are still lacking. Orthogonally detectable red, green, and blue fluorescent proteins combined in a single plasmid, dubbed RGB-S reporter, enable simultaneous, independent, and real-time analysis of the transcriptional response of Escherichia coli using three promoters which report physiological stress (PosmY for RpoS), genotoxicity (Psu

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Zhi, Liqiang, Jianwu Zhao, Hongmou Zhao, Zhong Qing, Hongliang Liu та Jianbing Ma. "Downregulation of LncRNA OIP5-AS1 Induced by IL-1β Aggravates Osteoarthritis via Regulating miR-29b-3p/PGRN". CARTILAGE, 10 лютого 2020, 194760351990080. http://dx.doi.org/10.1177/1947603519900801.

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Background Long noncoding RNA (lncRNA) OIP5 antisense RNA 1 (OIP5-AS1) is an oncogenic lncRNA; however, its role in osteoarthritis (OA) pathology still remains unknown. Materials and Methods qRT-PCR was performed to measure the expressions of OIP5-AS1, miR-29b-3p and progranulin (PGRN) mRNA in OA cartilage tissues and normal cartilage tissues. Chondrocyte cell lines, CHON-001 and ATDC5, were treated with different doses of interleukin-1β (IL-1β) to induce the inflammatory response. Overexpression plasmids, microRNA mimics, microRNA inhibitors and small interfering RNAs were constructed and tra

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Coffin, Carleton H., Luke A. Fisher, Sara Crippen, Phoebe Demers, Douglas H. Bartlett, and Catherine A. Royer. "Response and adaptation of the transcriptional heat shock response to pressure." Frontiers in Microbiology 15 (November 18, 2024). http://dx.doi.org/10.3389/fmicb.2024.1470617.

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IntroductionThe molecular mechanisms underlying pressure adaptation remain largely unexplored, despite their significance for understanding biological adaptation and improving sterilization methods in the food and beverage industry. The heat shock response leads to a global stabilization of the proteome. Prior research suggested that the heat shock regulon may exhibit a transcriptional response to high-pressure stress.MethodsIn this study, we investigated the pressure-dependent heat shock response in E. coli strains using plasmid-borne green fluorescent protein (GFP) promoter fusions and fluor

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Dissertations / Theses on the topic "PGREEN plasmid"

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Chuk, Oleksii Andriiovych. "Study experience of bacterial transformation by pGREEN plasmid." Thesis, Національний авіаційний університет, 2021. https://er.nau.edu.ua/handle/NAU/50621.

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Das S., Dash H. R. Microbial Biotechnology – A laboratory Manual for Bacterial Systems. New Delhi: Springer India, 2015. P. 35-72. DOI 10.1007/978-81-322-2095-4_2<br>The acquisition of practical skills in the genetic transformation of microorganisms is an integral part of the training of biotechnology students. The most common study practice is the integration of the green fluorescent protein (GFP) gene from jellyfish and the gene of ampicillin resistance into bacterial cells. The aim of the work was to adapt the standard procedure of bacterial transformation for educational purposes at the D

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Conference papers on the topic "PGREEN plasmid"

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Mudiyanselage, Chandana S. K. Herath, Neil Mitra, Dasuni Niyagama Gamage, et al. "Abstract 1145: Assessing the diagnostic value of the combination of Matrix Metalloproteinase 2 (MMP2) and Progranulin (PGRN) preoperative plasma levels for colorectal cancer (CRC)." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-1145.

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