Academic literature on the topic 'PHA productivity'

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Journal articles on the topic "PHA productivity"

1

Jin, Huanan, and Basil J. Nikolau. "Evaluating PHA Productivity of Bioengineered Rhodosprillum rubrum." PLoS ONE 9, no. 5 (2014): e96621. http://dx.doi.org/10.1371/journal.pone.0096621.

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2

Ruiz, Carolina, Shane T. Kenny, Ramesh Babu P, Meg Walsh, Tanja Narancic, and Kevin E. O’Connor. "High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440." Catalysts 9, no. 5 (2019): 468. http://dx.doi.org/10.3390/catal9050468.

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Waste cooking oil (WCO) is a major pollutant, primarily managed through incineration. The high cell density bioprocess developed here allows for better use of this valuable resource since it allows the conversion of WCO into biodegradable polymer polyhydroxyalkanoate (PHA). WCO was chemically hydrolysed to give rise to a mixture of fatty acids identical to the fatty acid composition of waste cooking oil. A feed strategy was developed to delay the stationary phase, and therefore achieve higher final biomass and biopolymer (PHA) productivity. In fed batch (pulse feeding) experiments Pseudomonas
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3

Blunt, Warren, David Levin, and Nazim Cicek. "Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity." Polymers 10, no. 11 (2018): 1197. http://dx.doi.org/10.3390/polym10111197.

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Microbial polyhydroxyalkanoates (PHAs) are promising biodegradable polymers that may alleviate some of the environmental burden of petroleum-derived polymers. The requirements for carbon substrates and energy for bioreactor operations are major factors contributing to the high production costs and environmental impact of PHAs. Improving the process productivity is an important aspect of cost reduction, which has been attempted using a variety of fed-batch, continuous, and semi-continuous bioreactor systems, with variable results. The purpose of this review is to summarize the bioreactor operat
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4

Maclean, Heather, Zhiyong Sun, Juliana Ramsay, and Bruce Ramsay. "Decaying exponential feeding of nonanoic acid for the production of medium-chain-length poly(3-hydroxyalkanoates) by Pseudomonas putida KT2440." Canadian Journal of Chemistry 86, no. 6 (2008): 564–69. http://dx.doi.org/10.1139/v08-062.

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Decaying exponential feeding strategies for the production of medium-chain-length poly(3-hydroxyalkanoates) by Pseudomonas putida KT2440 have been developed as a method of delaying oxygen limitation that is typically responsible for the termination of high cell density aerobic fermentations. Two feeding strategies were evaluated to control the biomass generation rate and consequently control the oxygen uptake rate of the cells. A linear decaying strategy allowed the production of 90 g L–1 dry biomass containing 65% PHA in 31 h with a cumulative productivity of 1.9 g L–1 h–1, while a quadratic
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5

Choi, Jong-il, Sang Yup Lee, and Kyuboem Han. "Cloning of the Alcaligenes latus Polyhydroxyalkanoate Biosynthesis Genes and Use of These Genes for Enhanced Production of Poly(3-hydroxybutyrate) in Escherichia coli." Applied and Environmental Microbiology 64, no. 12 (1998): 4897–903. http://dx.doi.org/10.1128/aem.64.12.4897-4903.1998.

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ABSTRACT Polyhydroxyalkanoates (PHAs) are microbial polyesters that can be used as completely biodegradable polymers, but the high production cost prevents their use in a wide range of applications. RecombinantEscherichia coli strains harboring the Ralstonia eutropha PHA biosynthesis genes have been reported to have several advantages as PHA producers compared with wild-type PHA-producing bacteria. However, the PHA productivity (amount of PHA produced per unit volume per unit time) obtained with these recombinantE. coli strains has been lower than that obtained with the wild-type bacterium Alc
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6

Takabatake, H., H. Satoh, T. Mino, and T. Matsuo. "Recovery of biodegradable plastics from activated sludge process." Water Science and Technology 42, no. 3-4 (2000): 351–56. http://dx.doi.org/10.2166/wst.2000.0402.

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In this research, PHA (polyhydroxyalkanoate) production system by activated sludge was studied. PHA behaves as carbon and energy storage material in bacteria. And PHA is a biodegradable plastic when extracted from bacteria. In this paper, the investigations from 3 aspects were reported; control of PHA composition, PHA production under coexistence of nitrogenous compounds, and influence of enrichment condition on PHA productivity. As results, it was possible to regulate PHA composition by utilizing acetate and propionate as carbon source and by regulating its composition. Nitrogenous compounds
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7

Chakraborty, Panchali, Kasiviswanathan Muthukumarappan, and William R. Gibbons. "PHA Productivity and Yield ofRalstonia eutrophaWhen Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids." Journal of Biomedicine and Biotechnology 2012 (2012): 1–8. http://dx.doi.org/10.1155/2012/506153.

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The research described in this present study was part of a larger effort focused on developing a dual substrate, dual fermentation process to produce Polyhydroxyalkanoate (PHA). The focus of this study was developing and optimizing a strategy for feeding a mixture of SCFAs (simulated ARF) and maximizing PHA production in a cost-effective way. Three different feeding strategies were examined in this study. The substrate evaluated in this study for the growth phase ofR. eutrophawas condensed corn solubles, a low-value byproduct of the dry-mill, corn ethanol industry. The culture was grown to hig
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8

Blunt, Warren, Christopher Dartiailh, Richard Sparling, Daniel J. Gapes, David B. Levin, and Nazim Cicek. "Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46." Bioengineering 6, no. 4 (2019): 89. http://dx.doi.org/10.3390/bioengineering6040089.

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High cell density (HCD) fed-batch cultures are widely perceived as a requisite for high-productivity polyhydroxyalkanoate (PHA) cultivation processes. In this work, a reactive pulse feed strategy (based on real-time CO2 or dissolved oxygen (DO) measurements as feedback variables) was used to control an oxygen-limited fed-batch process for improved productivity of medium chain length (mcl-) PHAs synthesized by Pseudomonas putida LS46. Despite the onset of oxygen limitation half-way through the process (14 h post inoculation), 28.8 ± 3.9 g L−1 total biomass (with PHA content up to 61 ± 8% cell d
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9

Carvalho, João M., Bruno C. Marreiros, and Maria A. M. Reis. "Polyhydroxyalkanoates Production by Mixed Microbial Culture under High Salinity." Sustainability 14, no. 3 (2022): 1346. http://dx.doi.org/10.3390/su14031346.

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The fishing industry produces vast amounts of saline organic side streams that require adequate treatment and disposal. The bioconversion of saline resources into value-added products, such as biodegradable polyhydroxyalkanoates (PHAs), has not yet been fully explored. This study investigated PHA production by mixed microbial cultures under 30 gNaCl/L, the highest NaCl concentration reported for the acclimatization of a PHA-accumulating mixed microbial culture (MMC). The operational conditions used during the culture-selection stage resulted in an enriched PHA-accumulating culture dominated by
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10

Palmeiro-Sánchez, Tania, José Luis Campos, and Anuska Mosquera-Corral. "Bioconversion of Organic Pollutants in Fish-Canning Wastewater into Volatile Fatty Acids and Polyhydroxyalkanoate." International Journal of Environmental Research and Public Health 18, no. 19 (2021): 10176. http://dx.doi.org/10.3390/ijerph181910176.

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The wastewater from the cookers of a tuna-canning plant was used as feedstock for the process. It was acidified in a continuous stirred tank reactor (CSTR) of 1.5 L to produce a mixture of volatile fatty acids (VFAs). The effluent contained 28.3 ± 8.7 g CODS/L and 25.0 ± 4.6 g CODVFA/L, 4.4 ± 1.6 g NH4+/L, and 10.9 ± 4.0 g Na+/L, which corresponds to about 28 g NaCl/L approximately. This was used to feed a PHA production system. The enriched MMC presented a capacity to accumulate PHAs from the fermented tuna wastewater. The maximum PHA content of the biomass in the fed-batch (8.35 wt% PHA) see
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