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Dissertations / Theses on the topic 'PHA synthases'

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Published: 4 June 2021

Last updated: 17 February 2022

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1

Thomas, Tatiana. "Étude du potentiel biotechnologique de Halomonas sp. SF2003 : application à la production de polyhydroxyalcanoates (PHA) Complete genome sequence of the halophilic PHA-producing bacterium Halomonas sp. SF2003: insights into its biotechnological potential PHA Production and PHA Synthases of the Halophilic Bacterium Halomonas sp. SF2003." Thesis, Lorient, 2019. http://www.theses.fr/2019LORIS542.

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L’amenuisement des ressources pétrochimiques couplé à la pollution engendrée par l’exploitation des plastiques posent de nombreuses questions et conduisent à un besoin urgent de solutions alternatives. Les polyhydroxyalcanoates (PHA) sont des polymères qui ont su se démarquer et naturellement s’imposer comme matériaux de remplacement, de par leurs caractères à la fois biosourcé et biodégradable. Leur synthèse par un grand nombre d’organismes procaryotes et eucaryotes, à partir d’une large gamme de substrats carbonés, rend leur production quasi illimitée et conduit à l’obtention de polymères au

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2

Ravelli, Thandara Garcia. "Seleção de genes codificadores de PHA sintases para a construção de recombinantes em Burkholderia sacchari e Pseudomonas sp e avaliação da produção de polihidroxialcanoatos com diferentes composições monoméricas." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-11062015-103546/.

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Polihidroxialcanoatos (PHA) são poliésteres acumulados por diversas bactérias a partir de fontes renováveis e são termoplásticos, biodegradáveis e biocompatíveis. A variabilidade da composição monomérica de PHA determina suas propriedades mecânicas e permite seu uso em diversas aplicações. A PHA sintase é a enzima responsável pela polimerização do PHA. O objetivo deste trabalho foi à busca por genes codificadores desta enzima, construção e avaliação de recombinantes portando tais genes. Inicialmente buscaram-se novos genes de PHA sintase a partir de clones de uma biblioteca metagenômica previa

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3

Gajdová, Barbora. "Identifikace PHA produkujících bakterií pomocí nástrojů molekulární biologie." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2017. http://www.nusl.cz/ntk/nusl-316230.

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This diploma thesis deals with identification of bacteria which are capable of producing polyhydroxyalkanoates (PHAs). Work included testing variety of genera including Pseudomonas, Lactobacillus, Bifidobacterium, thermophilic cultures and samples gathered from natural sources. Bacteria were investigated by molecular technique polymerase chain reaction – PCR. An amplification of the PHA synthase gene (phaC) was analyzed. In the first reaction phaC and 16S rRNA genes were tested at the same time. 16S rRNA gene is used as control for bacterial DNA and as an identification tool for natural source

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4

Kubáčková, Eliška. "Molekulárně biologická charakterizace vybraných producentů PHA." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2020. http://www.nusl.cz/ntk/nusl-414162.

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This diploma thesis focuses on the molecular characterization of selected PHA producers. Within this work, the PHA producing thermophilic isolates originating from the samples of activated sludge and compost were identified and characterized using molecular biological methods. By sequencing the 16S rRNA gene, the thermophilic isolates were identified and taxonomically classified into the Firmicutes bacterial phylum. In these bacterial isolates, the ability to produce PHA at the genotype level was determined by conventional PCR detection of the phaC gene encoding PHA synthase, which is a key en

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5

Caulkins, Juliana Carvalho de Arruda. "Identificação de genes envolvidos na síntese de polihidroxialcanoatos em Burkholderia cepacia linhagem IPT64." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-06082009-111247/.

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Os polihidroxialcanoatos (PHAs) são poliésteres acumulados por microrganismos como material de reserva. O conhecimento das vias bioquímicas e enzimas envolvidas na biossíntese e degradação dos PHAs é uma importante ferramenta para auxiliar na produção industrial. A linhagem Burkholderia cepacia IPT64 é capaz de acumular uma blenda composta de P(3HB) e P(3H4PE) a partir de sacarose. Este trabalho está focado em duas das principais enzimas envolvidas na biossíntese de PHAs: a b-cetotiolase (phaA) e a PHA sintase (phaC). A primeira está associada à especificidade pelo substrato, e a segunda é con

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6

Brondová, Zuzana. "Bioinformatická analýza PHA syntáz u termofilních bakterií." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-449376.

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The thesis deals with bioinformatics analysis, the aim of which was to find a suitable producer of PHA for new generation industrial biotechnologies from the collection of found thermophilic bacteria. Part of experiments was the finding of several thermophilic bacteria based on the similarity of the protein sequence of the phaC gene of the bacterium Cupriavidus necator. The next part of thesis was a literature search of the abilities of these thermophilic bacteria focused on culture conditions and the spectrum of usable substrates. Subsequently, five bacteria were selected for use in NGBI base

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7

Vlasáková, Terézia. "Isolace, identifikace a charakterizace extremofilů schopných produkce PHA." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2018. http://www.nusl.cz/ntk/nusl-376875.

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This diploma thesis is focused on isolation and identification of thermophilic microorganisms capable of production of polyhydroxyalkanoates (PHA) in the sample of activated sludge from wastewater treatment. 6 culture samples were isolated from activated sludge by means of cultivation technics and methods of molecular biology. They were closer specified by comparing nucleotide sequences of 16S-rRNA gene and assigned to bacterial genus Anoxybacillus. The production of PHA by this genus was not reported in literature so far. Samples were confirmed to contain phaC gene that codes the enzyme PHA-s

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8

Huttly, A. K. "Genes for ATP synthase subunits in pea chloroplast DNA." Thesis, University of Cambridge, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356654.

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9

Tomlinson, Kim Louise. "Starch synthesis in leaves of pea (Pisum sativum L.)." Thesis, University of East Anglia, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297009.

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10

Tian, Jiamin 1974. "Mechanistic investigation of polyhydroxybutyrate (PHB) synthases and elucidation of PHB biosynthesis and degradation process in Wautersia eutropha H16." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/30240.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 2005.<br>Includes bibliographical references.<br>Polyhydroxyalkanoate (PHA) synthase from various bacterial organisms is able to catalyze the polymerization of (R)-hydroxyalkanoate-CoAs into high molecular weight PHAs under nutrient- limited conditions in the presence of a carbon source. PHA synthases are representative of enzymes involved in polymerizations in which a soluble substrate is transformed into an insoluble inclusion during the polymerization process. The initiation, elongation, and termination phases of th

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11

Pinzón, Diana Carolina Tusso. "Caracterização do gene PHA sintase de bactérias isoladas a partir de amostras de solo." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-10122015-085901/.

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Os polihidroxialcanoatos (PHA) são poliésteres bacterianos. Na sua biossíntese, a PHA sintase incorpora monômeros 3HA à cadeia polimérica. O objetivo deste trabalho foi estudar o potencial da PHA sintase de 2 isolados do gênero Burkholderia sp. na produção de copolímeros. Construiram-se linhagens recombinantes que abrigavam os genes da PHA sintase classe I, em mutantes de Pseudomonas sp. e Burkholderia sacchari, que não acumulam PHA. Foram realizados ensaios de acúmulo de PHA usando glicose como fonte de carbono, apresentando a produção de unidades de 3HB, 3HO e 3HD nas linhagens recombinantes

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12

Dimitrov, Mauricio Rocha. "Construção de biblioteca metagenômica e prospecção de genes para a síntese de polihidroxalcanoatos." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-29042010-092836/.

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Os microrganismos constituem dois terços da diversidade biológica na Terra, no entanto, muitos deles não podem ser cultivados por técnicas tradicionais. Portanto, o acesso a esta diversidade tem sido feita através da utilização de técnicas independentes de cultivo. Diante deste panorama, a metagenômica apresenta-se como uma alternativa, pois dispensa a necessidade de cultivo. Tal técnica possibilita inclusive a identificação e utilização do potencial metabólico destes organismos para o desenvolvimento de novos processos e produtos. Os polihidroxialcanoatos (PHAs) são poliésteres bacterianos, a

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13

Jüngert, Janina Rachel [Verfasser], and Dieter [Akademischer Betreuer] Jendrossek. "Stringent response and phosphorylations of PHB synthase and PHB depolymerase influence the PHB content of Ralstonia eutropha H16 / Janina Rachel Jüngert ; Betreuer: Dieter Jendrossek." Stuttgart : Universitätsbibliothek der Universität Stuttgart, 2018. http://d-nb.info/1164444506/34.

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14

Krauser, Philipp Tibor [Verfasser]. "Effekt des Urämietoxin Phenylacetessigsäure (PAA) auf die Expression der induzierbaren NO-Synthase / Philipp Tibor Krauser." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2008. http://d-nb.info/1023261979/34.

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15

Wong, Mui-Yun. "Physiological and Molecular Analysis of Nitric Oxide Synthase During Bacterial Infection of Pea (Pisum sativum L.)." NCSU, 2004. http://www.lib.ncsu.edu/theses/available/etd-05202004-173922/.

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Nitric oxide (NO) and reactive oxygen species are two key components in the induction of the hypersensitive response (HR) during plant defense against pathogen infection. In animal cells, the production of NO is catalyzed by nitric oxide synthase (NOS). Although NOS activity has been documented in plants, the process of NO synthesis in plants is not well understood. Isolation of the NOS protein and/or cloning of the corresponding gene will greatly facilitate the understanding of NO synthesis and its role in plant defense. The objectives of this research were to analyze the physiological and bi

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16

Gomes, Rogério de Sousa. "Obtenção de mutantes deficientes no acúmulo de PHA e construção de linhagens recombinantes para o controle da composição monomérica." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-29042010-102817/.

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Pseudomonas putida produz PHA de cadeia média (PHAMCL) a partir de carboidratos e óleos vegetais. Genes de biossíntese de P3HB (poli-3-hidroxibutirato) de Ralstonia eutropha foram inseridos em P. putida IPT046 selvagem e mutantes PHA-. A expressão de phaC, codificador da PHA sintase, permitiu o acumulo de PHA com alto teor de 3HB, indicando que P. putida possui enzimas geradoras de 3HB. A expressão de phaB mostrou que seu produto canaliza 3HB e 3-hidroxihexanoato (3HHx) da <font face=\"Symbol\">&#946-oxidação para a PHA sintase. Ao expressar phaC em IPT461, afetado na PHA sintase, 30% de P3HB-

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Silva, Amanda Lys dos Santos. "Prospecção de microorganismos produtores de polihidroxialcanoatos e biosurfactantes em solo florestal e em lodo ativado." Universidade Federal de Alagoas, 2016. http://www.repositorio.ufal.br/handle/riufal/2140.

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Polyhydroxyalkanoates (PHAs) are polyesters produced and degradable by prokaryotes, while biosurfactants/bioemulsifiers (BS/BE) are metabolites that reduces surface tension and synthesized by this group of organisms. Interest in the potential industrial applications for PHA and BS/BE has increased due to its eco-friendly appeal. In this study, 24 bacterial colonies were isolated from Atlantic forest soil and agro-industrial sludge (Coruripe-AL, Brazil) in minimum mineral medium, as indicative of the possible ability to synthesize PHA. All strains were submitted to biochemical characterization,

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Costa, Tha??s Duarte. "Produ????o heter??loga de polihidroxialcanoato sintase (PhaC), biocatalisador da s??ntese de Poli (??cido l??tico) (PLA) em Komagataella phaffii." Universidade Cat??lica de Bras??lia, 2018. https://bdtd.ucb.br:8443/jspui/handle/tede/2406.

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Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-06-06T14:01:03Z No. of bitstreams: 1 ThaisDuarteCostaDissertacao2018.pdf: 3076865 bytes, checksum: 13af7d694f07d7e2dcc9281907285b62 (MD5)<br>Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2018-06-06T14:01:33Z (GMT) No. of bitstreams: 1 ThaisDuarteCostaDissertacao2018.pdf: 3076865 bytes, checksum: 13af7d694f07d7e2dcc9281907285b62 (MD5)<br>Made available in DSpace on 2018-06-06T14:01:33Z (GMT). No. of bitstreams: 1 ThaisDuarteCostaDissertacao2018.pdf: 3076865 bytes, checksum: 13af7d694f07d7e2dcc9281907285b62 (M

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Peters, Verena. "Polyester synthases and polyester granule assembly : a thesis presented to Massey University in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Microbiology." 2008. http://hdl.handle.net/10179/808.

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PHAs are a class of biopolymers consisting of (R)-3-hydroxy-fatty acids and are produced by the majority of eubacteria and some archaeal bacteria as carbon storage material. In general, PHA is synthesised when a carbon source is available in excess while another essential nutrient is limited. The key enzyme of PHA biosynthesis, the PHA synthase, catalyses the polymerisation of the substrate (R)-3-hydroxyacyl-CoA to PHA accompanied by the release of coenzyme A. PHA is stored intracellularly as inclusions, the so-called PHA granules. When the external carbon source becomes exhausted, bacteria ca

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Dartiailh, Christopher Neil Bergman. "Analyzing polyhydroxyalkanoate accumulation and identifying the PHA synthase gene, phaC, in Thermus sp." 2013. http://hdl.handle.net/1993/22013.

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Polyhydroxyalkanoate (PHA) is a cellular storage polymer with similar properties to some plastics when extracted from the cell and acts as a renewable and biodegradable plastic alternative. The objective of this research was to identify thermophilic PHA producing microorganisms and characterize their PHA accumulation, by which the process may become more competitive to current petroleum-based plastic production. This study characterizes growth and PHA production of Thermus thermophilus HB8 and three strains of T. filiformis (MOK, NGM and WRT), while simultaneously performing a genetic analysi

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21

Lai, Yung-Wei, and 賴勇維. "Protein engineering study on the thermostability of PHA synthase from Cupriavidus sp. S6." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/49838669018257747601.

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碩士<br>國立高雄海洋科技大學<br>海洋生物技術研究所<br>98<br>Thermophile Cupriavidus sp. S-6 accumulates polyhydroxybutyrate from glucose at its optimal growth temperature 50oC. The PHA synthase enzymes of Cupriavidus sp. S-6 (PhaCCsp) and C. necator H16 (PhaCH16) shared overall 81% identities. In this study, domain swapping, site-directed mutagenesis and DNA shuffling were used to investigate the key amino acid, which improve the thermal stability of PHA synthase. Domain swapping experiments confirmed that the  region of thermophile PHA synthase enhanced the thermal stability of mesophile PHA synthase. Site-direc

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Huang, Yan-Jia, and 黃筵嘉. "Screening polyhydroxyalkanoate producer with unique composition and analyzing substrate specificity of the PHA synthase." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/92631143180947412232.

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碩士<br>國立臺灣大學<br>農業化學研究所<br>93<br>We screened the poly(3-hydroxyalkanoates) (PHAs) producers by cultivated in MSM containing 0.5 µg/ml Nile red from environment. Nine PHA producers had been isolated and verified for their PHA accumulation ability by flask fermentation and gas chromatography analysis. Among the wild type strains, TO7, PO6, PO21, TS18, and TF1-1 possess high PHA accumulation ability. The monomer compositions of PHAs produced by PO6 and PO21 possessed short-chain-length (scl) and medium- chain-length (mcl) monomers. The mcl monomer mol% of PHA produced by PO6 and PO21 could be reg

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Ho, Meng Fan, and 何夢凡. "The study of the key amino acid residues simultaneously enhances the thermostability and enzyme activity of PHA synthase." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/33msa4.

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24

LIU, CHUNG-HSIEN, and 劉崇賢. "The Study of Pseudomonas putida H9 Biosynthesized Hompolymer Poly(3-hydroxyhexanoate) and the Substrate Specificity of PHA Synthase." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/cnfgv8.

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碩士<br>國立高雄海洋科技大學<br>海洋生物技術研究所<br>106<br>Pseudomonas putida H9, a wild type bacteria isolated from soil sampling from Southern Taiwan, is capable of accumulating 48 dry wt% polyhydroxyalkanoate with 92 mol% 3-hydroxyhexanoate using sodium hexanoate as sole carbon source. P. putida H9 grew and accumulated PHA on a broad range of pH and without an obviously optimal pH. Medium with a C/N 30 was the best condition for PHA accumulation. P. putida H9 had three PHA synthases, phaC1H9, phaC2H9, and phbCH9. Deletion of phaC1 gene led to P. pudtida H9 losing the PHA accumulation ability completely. Delet

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Chung, Ting, and 鍾婷. "Cloning and studying genes encoded hydrolase and PHB synthase from microorganisms." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/89738071577014203118.

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碩士<br>元智大學<br>生物科技與工程研究所<br>96<br>Polyhydroxybutyrate (PHB) is polyester accumulated by various bacteria under unbalanced growth conditions when the carbon substrate is in excess to other nutrients such as nitrogen, sulfur, phosphorus or oxygen. Of the variety of bacteria that synthesize PHB, Cupriavidus necator H16 (formally known as Ralstonia eutropha H16) is the most intensively studied, and the genes involved in PHA metabolism in this bacterium have been well characterized. PHB operon of C. necator H16 has been cloned and introduced into Escherichia coli as PHB-producing recombinant strai

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26

Tsai, Pei-Chien, and 蔡佩倩. "Identification of the amino acid residues that change the substrate specificity of PHA synthase 1 from Pseudomonas putida GPo1." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/05311529025531561421.

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碩士<br>國立臺灣大學<br>農業化學研究所<br>92<br>In our previously study, the substrate specificity of polyhydroxyalkanoate (PHA) synthase 1 (class II) from Pseudomonas putida GPo1 was successfully altered by localized semi-random mutagenesis. The evolved enzyme PS-E1, in which 14 amino acids had been changed exhibited broad substrate specificity. The promising amino acid positions which change the substrate specificity of PhaC1Pp were investigated by site directed mutagenesis. Mutations at L484V or S325C were remarkably enhanced the short-chain-length monomer composition up to 40 mol% in PHA accumulation exp

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Hsieh, Shu-Ping, and 謝舒萍. "The study of polyhydroxyalkanoate accumulation in marine bacteria Paracoccus stylophorae KTW-16 and the cloning of the PHA synthase gene." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/44999840767248177832.

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碩士<br>國立高雄海洋科技大學<br>海洋生物技術研究所<br>100<br>The marine bacterium, Paracoccus stylophorae KTW-16, was isolated from the coral Stylophora pistillata, collected from the Kenting sea waters southern Taiwan. In the marine broth (MB) without extra carbon source, P. stylophorae KTW-16 was capable of synthesizing 80 mol% 3-hydroxybutyrate (3-HB) and 20 mol% 3-hydroxyvalerate (3-HV) of PHA copolymer. At 25oC, P. stylophorae KTW-16 accumulated 30% PHA with 75 mol% 3-HB and 25 mol% 3-HV. The 3-HV monomer composition of PHA was decreased with the increase of tryptone in MB. According to this result, the effec

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Lin, Tzu-Line, and 林姿伶. "Cloning and analysis of Polyhydroxyallkanoate synthase gene (phaC) from the extreme haloarchaea-Haloarcula sp. strain HLR2." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/57645891097508472778.

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碩士<br>國立中興大學<br>生命科學系<br>94<br>Many microorganisms have been demonstrated to transform and accumulate the excess carbon source into the polyhydroxyalkanoate (PHA) granules as energy storage materials while encountering the nutrient-limiting stress. When the supply of the limiting nutrient is restored, the PHA can be depolymerized and subsequently metabolized as carbon and energy source with acetyl-CoA as intermediate. Extreme halophilic archaeon- Haloarcula strain HLR2 was isolated from the salt crystals of the salt mountain at Miao-Li, Taiwan. The unique triangle shape of strain HLR2 grew at

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Jahns, Anika Carolin. "Towards a better understanding of the polyhydroxyalkanoate synthase from Ralstonia eutropha : protein engineering and molecular biometrics : a thesis presented to Massey University in partial fulfilment of the requirement for the degree of Doctor of Philosophy in Microbiology." 2009. http://hdl.handle.net/10179/1081.

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Polyhydroxyalkanoates (PHAs) are polyesters composed of (R)-3-hydroxy-fatty acids. A variety of gram-positive as well as gram-negative bacteria and some archaea are able to produce these biopolymers as energy and carbon storage materials. In times of unbalanced growth, when carbon is available in excess but other nutrients are limited, PHA inclusions are formed. These granules are water-insoluble, stored intracellularly and can be maintained outside the cell as beads. The key enzyme for the formation of PHA inclusions is the PHA synthase PhaC, which catalyses the polymerization of (R)- 3-hydro

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Sherteel, Rajaa. "Metabolic Engineering to Improve Biohydrogen Production by Rhodobacter capsulatus JP91." Thèse, 2017. http://hdl.handle.net/1866/20525.

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