Relevant bibliographies by topics / PhaC synthase genes

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Academic literature on the topic 'PhaC synthase genes'

Author: Grafiati
Published: 4 June 2025
Last updated: 1 August 2025

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Journal articles on the topic "PhaC synthase genes"

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Ulbrich, P., H. Strnad, V. Hejkalová, J. Pačes, and Václav Pačes. "Genetic Determination of Polyhydroxyalkanoate Metabolism in Rhodobacter capsulatus SB1003." Folia Biologica 48, no. 4 (2002): 157–59. https://doi.org/10.14712/fb2002048040157.

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A cluster of four genes was identified in the Rhodobacter capsulatus genome that is involved in PHA metabolism. These genes encode the PHA granule-associated protein (pha2), the regulator for granule formation (pha1), the PHA synthase (phaC) and the PHA depolymerase (orfX). Two other genes, namely those encoding β-ketothiolase (phaA) and acetoacetyl-CoA reductase (phaB), are not linked to this cluster.
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McCool, Gabriel J., and Maura C. Cannon. "PhaC and PhaR Are Required for Polyhydroxyalkanoic Acid Synthase Activity in Bacillus megaterium." Journal of Bacteriology 183, no. 14 (2001): 4235–43. http://dx.doi.org/10.1128/jb.183.14.4235-4243.2001.

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ABSTRACT Polyhydroxyalkanoic acids (PHAs) are a class of polyesters stored in inclusion bodies and found in many bacteria and in some archaea. The terminal step in the synthesis of PHA is catalyzed by PHA synthase. Genes encoding this enzyme have been cloned, and the primary sequence of the protein, PhaC, is deduced from the nucleotide sequences of more than 30 organisms. PHA synthases are grouped into three classes based on substrate range, molecular mass, and whether or not there is a requirement for phaE in addition to thephaC gene product. Here we report the results of an analysis of a PHA
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Han, Jing, Qiuhe Lu, Ligang Zhou, Jian Zhou, and Hua Xiang. "Molecular Characterization of the phaECHm Genes, Required for Biosynthesis of Poly(3-Hydroxybutyrate) in the Extremely Halophilic Archaeon Haloarcula marismortui." Applied and Environmental Microbiology 73, no. 19 (2007): 6058–65. http://dx.doi.org/10.1128/aem.00953-07.

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ABSTRACT Although many haloarchaea produce biodegradable polyhydroxyalkanoates (PHAs), the genes involved in PHA synthesis in the domain of Archaea have not yet been experimentally investigated yet. In this study, we revealed that Haloarcula marismortui was able to accumulate poly(3-hydroxybutyrate) (PHB) up to 21% of cellular dry weight when cultured in a minimal medium with excessive glucose and identified the phaE Hm and phaC Hm genes, probably encoding two subunits of a class III PHA synthase. These two genes were adjacent and directed by a single promoter located 26 bp upstream of the tra
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York, Gregory M., JoAnne Stubbe, and Anthony J. Sinskey. "The Ralstonia eutropha PhaR Protein Couples Synthesis of the PhaP Phasin to the Presence of Polyhydroxybutyrate in Cells and Promotes Polyhydroxybutyrate Production." Journal of Bacteriology 184, no. 1 (2002): 59–66. http://dx.doi.org/10.1128/jb.184.1.59-66.2002.

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ABSTRACT Polyhydroxyalkanoates (PHAs) are polyoxoesters that are produced by many bacteria and that accumulate as intracellular granules. Phasins (PhaP) are proteins that accumulate during PHA synthesis, bind PHA granules, and promote further PHA synthesis. Interestingly, PhaP accumulation seems to be strictly dependent on PHA synthesis, which is catalyzed by the PhaC PHA synthase. Here we have tested the effect of the Ralstonia eutropha PhaR protein on the regulation of PhaP accumulation. R. eutropha strains with phaR, phaC, and/or phaP deletions were constructed, and PhaP accumulation was me
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Korotkova, Natalia, та Mary E. Lidstrom. "Connection between Poly-β-Hydroxybutyrate Biosynthesis and Growth on C1 and C2 Compounds in the Methylotroph Methylobacterium extorquensAM1". Journal of Bacteriology 183, № 3 (2001): 1038–46. http://dx.doi.org/10.1128/jb.183.3.1038-1046.2001.

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ABSTRACT Several DNA regions containing genes involved in poly-β-hydroxybutyrate (PHB) biosynthesis and degradation and also in fatty acid degradation were identified from genomic sequence data and have been characterized in the serine cycle facultative methylotrophMethylobacterium extorquens AM1. Genes involved in PHB biosynthesis include those encoding β-ketothiolase (phaA), NADPH-linked acetoacetyl coenzyme A (acetyl-CoA) reductase (phaB), and PHB synthase (phaC).phaA and phaB are closely linked on the chromosome together with a third gene with identity to a regulator of PHB granule-associa
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Niamsiri, Nuttawee, Soazig C. Delamarre, Young-Rok Kim, and Carl A. Batt. "Engineering of Chimeric Class II Polyhydroxyalkanoate Synthases." Applied and Environmental Microbiology 70, no. 11 (2004): 6789–99. http://dx.doi.org/10.1128/aem.70.11.6789-6799.2004.

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ABSTRACT PHA synthase is a key enzyme involved in the biosynthesis of polyhydroxyalkanoates (PHAs). Using a combinatorial genetic strategy to create unique chimeric class II PHA synthases, we have obtained a number of novel chimeras which display improved catalytic properties. To engineer the chimeric PHA synthases, we constructed a synthetic phaC gene from Pseudomonas oleovorans (phaC1 Po ) that was devoid of an internal 540-bp fragment. Randomly amplified PCR products (created with primers based on conserved phaC sequences flanking the deleted internal fragment) were generated using genomic
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Lu, Qiuhe, Jing Han, Ligang Zhou, Jian Zhou, and Hua Xiang. "Genetic and Biochemical Characterization of the Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Synthase in Haloferax mediterranei." Journal of Bacteriology 190, no. 12 (2008): 4173–80. http://dx.doi.org/10.1128/jb.00134-08.

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ABSTRACT The haloarchaeon Haloferax mediterranei has shown promise for the economical production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a desirable bioplastic. However, little is known at present about the genes involved in PHBV synthesis in the domain Archaea. In this study, we cloned the gene cluster (phaEC Hme) encoding a polyhydroxyalkanoate (PHA) synthase in H. mediterranei CGMCC 1.2087 via thermal asymmetric interlaced PCR. Western blotting revealed that the phaE Hme and phaC Hme genes were constitutively expressed, and both the PhaEHme and PhaCHme proteins were strongly
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Hai, Tran, Daniela Lange, Ralf Rabus, and Alexander Steinbüchel. "Polyhydroxyalkanoate (PHA) Accumulation in Sulfate-Reducing Bacteria and Identification of a Class III PHA Synthase (PhaEC) in Desulfococcus multivorans." Applied and Environmental Microbiology 70, no. 8 (2004): 4440–48. http://dx.doi.org/10.1128/aem.70.8.4440-4448.2004.

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ABSTRACT Seven strains of sulfate-reducing bacteria (SRB) were tested for the accumulation of polyhydroxyalkanoates (PHAs). During growth with benzoate Desulfonema magnum accumulated large amounts of poly(3-hydroxybutyrate) [poly(3HB)]. Desulfosarcina variabilis (during growth with benzoate), Desulfobotulus sapovorans (during growth with caproate), and Desulfobacterium autotrophicum (during growth with caproate) accumulated poly(3HB) that accounted for 20 to 43% of cell dry matter. Desulfobotulus sapovorans and Desulfobacterium autotrophicum also synthesized copolyesters consisting of 3-hydrox
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Han, Jing, Jing Hou, Hailong Liu, et al. "Wide Distribution among Halophilic Archaea of a Novel Polyhydroxyalkanoate Synthase Subtype with Homology to Bacterial Type III Synthases." Applied and Environmental Microbiology 76, no. 23 (2010): 7811–19. http://dx.doi.org/10.1128/aem.01117-10.

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ABSTRACT Polyhydroxyalkanoates (PHAs) are accumulated as intracellular carbon and energy storage polymers by various bacteria and a few haloarchaea. In this study, 28 strains belonging to 15 genera in the family Halobacteriaceae were investigated with respect to their ability to synthesize PHAs and the types of their PHA synthases. Fermentation results showed that 18 strains from 12 genera could synthesize polyhydroxybutyrate (PHB) or poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). For most of these haloarchaea, selected regions of the phaE and phaC genes encoding PHA synthases (type III)
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Sharma, Parveen, Riffat Munir, Jocelyn Plouffe, Nidhi Shah, Teresa Kievit, and David Levin. "Polyhydroxyalkanoate (PHA) Polymer Accumulation and pha Gene Expression in Phenazine (phz-) and Pyrrolnitrin (prn-) Defective Mutants of Pseudomonas chlororaphis PA23." Polymers 10, no. 11 (2018): 1203. http://dx.doi.org/10.3390/polym10111203.

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Pseudomonas chlororaphis PA23 was isolated from the rhizosphere of soybeans and identified as a biocontrol bacterium against Sclerotinia sclerotiorum, a fungal plant pathogen. This bacterium produces a number of secondary metabolites, including phenazine-1-carboxylic acid, 2-hydroxyphenazine, pyrrolnitrin (PRN), hydrogen cyanide, proteases, lipases and siderophores. It also synthesizes and accumulates polyhydroxyalkanoate (PHA) polymers as carbon and energy storage compounds under nutrient-limited conditions. Pseudomonads like P. chlororaphis metabolize glucose via the Entner-Doudoroff and Pen
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Dissertations / Theses on the topic "PhaC synthase genes"

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Brondová, Zuzana. "Bioinformatická analýza PHA syntáz u termofilních bakterií." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2021. http://www.nusl.cz/ntk/nusl-449376.

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The thesis deals with bioinformatics analysis, the aim of which was to find a suitable producer of PHA for new generation industrial biotechnologies from the collection of found thermophilic bacteria. Part of experiments was the finding of several thermophilic bacteria based on the similarity of the protein sequence of the phaC gene of the bacterium Cupriavidus necator. The next part of thesis was a literature search of the abilities of these thermophilic bacteria focused on culture conditions and the spectrum of usable substrates. Subsequently, five bacteria were selected for use in NGBI base
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Ravelli, Thandara Garcia. "Seleção de genes codificadores de PHA sintases para a construção de recombinantes em Burkholderia sacchari e Pseudomonas sp e avaliação da produção de polihidroxialcanoatos com diferentes composições monoméricas." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-11062015-103546/.

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Polihidroxialcanoatos (PHA) são poliésteres acumulados por diversas bactérias a partir de fontes renováveis e são termoplásticos, biodegradáveis e biocompatíveis. A variabilidade da composição monomérica de PHA determina suas propriedades mecânicas e permite seu uso em diversas aplicações. A PHA sintase é a enzima responsável pela polimerização do PHA. O objetivo deste trabalho foi à busca por genes codificadores desta enzima, construção e avaliação de recombinantes portando tais genes. Inicialmente buscaram-se novos genes de PHA sintase a partir de clones de uma biblioteca metagenômica previa
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Dimitrov, Mauricio Rocha. "Construção de biblioteca metagenômica e prospecção de genes para a síntese de polihidroxalcanoatos." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-29042010-092836/.

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Os microrganismos constituem dois terços da diversidade biológica na Terra, no entanto, muitos deles não podem ser cultivados por técnicas tradicionais. Portanto, o acesso a esta diversidade tem sido feita através da utilização de técnicas independentes de cultivo. Diante deste panorama, a metagenômica apresenta-se como uma alternativa, pois dispensa a necessidade de cultivo. Tal técnica possibilita inclusive a identificação e utilização do potencial metabólico destes organismos para o desenvolvimento de novos processos e produtos. Os polihidroxialcanoatos (PHAs) são poliésteres bacterianos, a
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Pinzón, Diana Carolina Tusso. "Caracterização do gene PHA sintase de bactérias isoladas a partir de amostras de solo." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-10122015-085901/.

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Os polihidroxialcanoatos (PHA) são poliésteres bacterianos. Na sua biossíntese, a PHA sintase incorpora monômeros 3HA à cadeia polimérica. O objetivo deste trabalho foi estudar o potencial da PHA sintase de 2 isolados do gênero Burkholderia sp. na produção de copolímeros. Construiram-se linhagens recombinantes que abrigavam os genes da PHA sintase classe I, em mutantes de Pseudomonas sp. e Burkholderia sacchari, que não acumulam PHA. Foram realizados ensaios de acúmulo de PHA usando glicose como fonte de carbono, apresentando a produção de unidades de 3HB, 3HO e 3HD nas linhagens recombinantes
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Caulkins, Juliana Carvalho de Arruda. "Identificação de genes envolvidos na síntese de polihidroxialcanoatos em Burkholderia cepacia linhagem IPT64." Universidade de São Paulo, 2008. http://www.teses.usp.br/teses/disponiveis/87/87131/tde-06082009-111247/.

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Os polihidroxialcanoatos (PHAs) são poliésteres acumulados por microrganismos como material de reserva. O conhecimento das vias bioquímicas e enzimas envolvidas na biossíntese e degradação dos PHAs é uma importante ferramenta para auxiliar na produção industrial. A linhagem Burkholderia cepacia IPT64 é capaz de acumular uma blenda composta de P(3HB) e P(3H4PE) a partir de sacarose. Este trabalho está focado em duas das principais enzimas envolvidas na biossíntese de PHAs: a b-cetotiolase (phaA) e a PHA sintase (phaC). A primeira está associada à especificidade pelo substrato, e a segunda é con
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Dartiailh, Christopher Neil Bergman. "Analyzing polyhydroxyalkanoate accumulation and identifying the PHA synthase gene, phaC, in Thermus sp." 2013. http://hdl.handle.net/1993/22013.

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Polyhydroxyalkanoate (PHA) is a cellular storage polymer with similar properties to some plastics when extracted from the cell and acts as a renewable and biodegradable plastic alternative. The objective of this research was to identify thermophilic PHA producing microorganisms and characterize their PHA accumulation, by which the process may become more competitive to current petroleum-based plastic production. This study characterizes growth and PHA production of Thermus thermophilus HB8 and three strains of T. filiformis (MOK, NGM and WRT), while simultaneously performing a genetic analysi
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Lin, Tzu-Line, and 林姿伶. "Cloning and analysis of Polyhydroxyallkanoate synthase gene (phaC) from the extreme haloarchaea-Haloarcula sp. strain HLR2." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/57645891097508472778.

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碩士<br>國立中興大學<br>生命科學系<br>94<br>Many microorganisms have been demonstrated to transform and accumulate the excess carbon source into the polyhydroxyalkanoate (PHA) granules as energy storage materials while encountering the nutrient-limiting stress. When the supply of the limiting nutrient is restored, the PHA can be depolymerized and subsequently metabolized as carbon and energy source with acetyl-CoA as intermediate. Extreme halophilic archaeon- Haloarcula strain HLR2 was isolated from the salt crystals of the salt mountain at Miao-Li, Taiwan. The unique triangle shape of strain HLR2 grew at
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Hsieh, Shu-Ping, and 謝舒萍. "The study of polyhydroxyalkanoate accumulation in marine bacteria Paracoccus stylophorae KTW-16 and the cloning of the PHA synthase gene." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/44999840767248177832.

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碩士<br>國立高雄海洋科技大學<br>海洋生物技術研究所<br>100<br>The marine bacterium, Paracoccus stylophorae KTW-16, was isolated from the coral Stylophora pistillata, collected from the Kenting sea waters southern Taiwan. In the marine broth (MB) without extra carbon source, P. stylophorae KTW-16 was capable of synthesizing 80 mol% 3-hydroxybutyrate (3-HB) and 20 mol% 3-hydroxyvalerate (3-HV) of PHA copolymer. At 25oC, P. stylophorae KTW-16 accumulated 30% PHA with 75 mol% 3-HB and 25 mol% 3-HV. The 3-HV monomer composition of PHA was decreased with the increase of tryptone in MB. According to this result, the effec
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Scholz, Anja [Verfasser]. "Expression bakterieller Gene zur PHA-Synthese in Arxula adeninivorans und Untersuchungen zum Stoffwechsel der Hefe / vorgelegt von Anja Scholz." 2008. http://d-nb.info/996777385/34.

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Book chapters on the topic "PhaC synthase genes"

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Schubert, Peter, Andreas Pries, Niels Krüger, and Alexander Steinbüchel. "Molecular analysis of the Alcaligenes eutrophus PHB-biosynthetic genes: identification of the NH2-terminus of PHB synthase and identification of the transcription start site of phbC." In Novel Biodegradable Microbial Polymers. Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-2129-0_42.

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S. Alwuhaib, Aisha, Vitaly Zinkevich, Tamar Kartvelishvili, Nino Asatiani, and Nelly Sapojnikova. "Bioplastics against Microplastics: Screening of Environmental Bacteria for Bioplastics Production." In Environmental Sciences. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.109756.

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Polyhydroxyalkanoates (PHAs) are biopolymers produced by numerous bacteria and can be used in the production of bioplastics. PHAs are synthesized by microorganisms by fermentation of carbon sources. Due to the different monomer structures of PHAs, there are many kinds of PHAs, and their corresponding material properties are also very different. Thus, the search for bacteria producing the PHAs is of great interest. In this study, the bacteria isolated from the environment were analyzed for the presence of PHA. PHA production was tested with staining methods Sudan Black B, Nile Blue, and Nile Re
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Malik, Javid A., and Monika Bhadauria. "Polyhydroxyalkanoates." In Handbook of Research on Environmental and Human Health Impacts of Plastic Pollution. IGI Global, 2020. http://dx.doi.org/10.4018/978-1-5225-9452-9.ch018.

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Human dependence on number of chemicals or chemical derivatives has increased alarmingly. Among the commodity chemicals, plastics are becoming independent for our modern lifestyle, as the usage of plastics is increasing worryingly. However, these synthetic plastics are extremely persistent in nature and accumulate in the environment, thereby leading to serious ecological problems. So, to build our economy sustainably, a need of replacement is necessary. Biomaterials in terms of bioplastics are an anticipated option, being synthesized and catabolized by different organisms with myriad biotechno
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Vlachopapadopoulou, Elpis-Athina, and Myrto Bonataki. "Diagnosis of Hypoaldosteronism in Infancy." In Renin-Angiotensin Aldosterone System [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.97448.

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Hypoaldosteronism is associated with either insufficient aldosterone production or lack of responsiveness to aldosterone and can be isolated or in the context of primary adrenal failure. Τhe severity of clinical manifestations is inversely correlated to age, with the neonatal period being the most vulnerable time for a patient to present with mineralocorticoid insufficiency. Salt-wasting forms of congenital adrenal hyperplasia (CAH), adrenal hypoplasia congenita (AHC), aldosterone synthase deficiency (ASD) and pseudohypoaldosteronism (PHA) are all causes of hypoaldosteronism in infancy. Affect
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Reports on the topic "PhaC synthase genes"

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Dudareva, Natalia, Alexander Vainstein, Eran Pichersky, and David Weiss. Integrating biochemical and genomic approaches to elucidate C6-C2 volatile production: improvement of floral scent and fruit aroma. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7696514.bard.

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The specific objectives of approved proposal include to: 1. Elucidate the C6-C2 biochemical pathways leading to the biosynthesis of phenylacetaldehyde, phenylethyl alcohol and phenylethyl acetate in floral tissues of ornamentally important plants, pefunia and roses. 2. Isolate and characterrze genes responsible for the production of these C6-C2 compounds and those involved in the regulation of the pathway using genomic and transcriptomic tools. 3. Determine whether altering the expression of key genes of this pathway can result in changing the aroma characteristics of flowers. Aldehydes are in
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