Relevant bibliographies by topics / Phaeodactylum tricornutum

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Academic literature on the topic 'Phaeodactylum tricornutum'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 May 2024

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Journal articles on the topic "Phaeodactylum tricornutum"

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Bowler, Chris, and Angela Falciatore. "Phaeodactylum tricornutum." Trends in Genetics 35, no. 9 (September 2019): 706–7. http://dx.doi.org/10.1016/j.tig.2019.05.007.

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Veloso, V., A. Reis, L. Gouveia, H. L. Fernandes, J. A. Empis, and J. M. Novais. "Lipid production by Phaeodactylum tricornutum." Bioresource Technology 38, no. 2-3 (January 1991): 115–19. http://dx.doi.org/10.1016/0960-8524(91)90141-6.

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Sudo, Yoshitaka, Hajime Okawara, and Eiji O'Shima. "Growth Rate of Phaeodactylum tricornutum." KAGAKU KOGAKU RONBUNSHU 17, no. 3 (1991): 484–90. http://dx.doi.org/10.1252/kakoronbunshu.17.484.

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Burch, Andrew R., Cody W. Yothers, Michelle R. Salemi, Brett S. Phinney, Pramod Pandey, and Annaliese K. Franz. "Quantitative label-free proteomics and biochemical analysis of Phaeodactylum tricornutum cultivation on dairy manure wastewater." Journal of Applied Phycology 33, no. 4 (May 27, 2021): 2105–21. http://dx.doi.org/10.1007/s10811-021-02483-3.

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AbstractMicroalgae cultivation on wastewater offers the dual benefit of lowering costs for feedstock production with simultaneous wastewater remediation. This study utilized biochemical and quantitative label-free proteomic approaches to evaluate the growth and proteomic response for diatom Phaeodactylum tricornutum cultivated on flushed dairy manure wastewater (DMW). Comparing several DMW dilutions (up to 60% DMW diluted in seawater) with a synthetic seawater medium indicates that biomass and lipid yields correlate with the starting nitrogen content of the DMW dilution. Phaeodactylum tricornutum cultivated on DMW exhibits elevated levels of polyunsaturated fatty acids (PUFAs), particularly docosapentaenoic acid (DPA, 22:5 n-3). Proteomic analysis revealed alterations in the regulations of proteins associated with protein metabolism, cellular signaling, transcription and translation, protein trafficking, and oxidative stress management pathways when comparing P. tricornutum cultivation on diluted DMW versus synthetic media, thus providing insights into how P. tricornutum reorganizes its proteome in response to a complex wastewater source. Graphical abstract
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Ak, Burcu, Gökhan Tamer Kayaalp, Oya Işık, and Melis Çelik Güney. "Phaeodactylum Trıcornutum Kültürlerinde Biyomas Tahmini." Turkish Journal of Agriculture - Food Science and Technology 5, no. 2 (February 28, 2017): 182. http://dx.doi.org/10.24925/turjaf.v5i2.182-184.1145.

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Phaeodactylum tricornutum is the microalgae that is known to produce lipid. In this study, it was aimed to estimate the microalgae Phaeodactylum tricornutum dry matter cultured in the conditions of nitrogen deficiency as a stress factor, in outdoor, in photo bioreactors, by using multiple regression analysis method. In this study, diatom Phaeodactylum tricornutum (Bohlin) was cultured medium of which N was reduced by 50% and with 20% inoculation ratio. The light intensity, temperature, optical density and chlorophyll a, were measured daily. The mathematical model was formed for control group and 50 % of the N applied to the group using optical density, temperature, light intensity, chlorophyll a and without chlorophyll a. The regression equation of control group was estimated. The R2 value of control group was found 95.1% and statistically significant. Then, the regression equation was estimated for control group without chlorophyll a. The R2 value of this equation was found 94.0% and statistically significant. And then, the regression equation was estimated in 50% nitrogen. The R2 value of control group was found 92.4% and statistically significant. Finally, the regression equation was estimated in 50% nitrogen deficiency without chlorophyll a. The R2 value of this equation was found 91.7% and statistically significant.
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Li, Si, and Stefanie M. H. Ismar. "Transcriptome, Biochemical and Growth Responses of the Marine Phytoplankter Phaeodactylum Tricornutum Bohlin (Bacillariophyta) to Copepod Grazer Presence." Cellular Physiology and Biochemistry 46, no. 3 (2018): 1091–111. http://dx.doi.org/10.1159/000488839.

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Background/Aims: As a model organism for a pleiomorphic marine planktonic primary producer, Phaeodactylum tricornutum has been studied on a molecular level under diverse cultural conditions. But little is known about its morphological, nutritional or transcriptomic responses under grazing stress. Methods: To assess microalgal molecular and cellular responses to grazer presence, we conducted transcriptome profiling in combination with growth rate, biovolume, fatty acid content, carbon and nitrogen content measurements in the model diatom Phaeodactylum tricornutum. RNA-sequencing was used to evaluate the transcriptomic response to grazing stress for P. tricornutum strain CCAP 1055/1. Results: Among the differentially expressed genes, we found down-regulation of genes involved in pathogen resistance, and in fatty acid biosynthesis pathways, while mitosis-involved genes were up-regulated. Experimentally testing morphological and biochemical responses in five strains of the species, we detected strain-specific significant effects of simulated grazing pressure in altered growth rates, biovolume and nutritional composition. Conclusion: Our research reveals the associated molecular and cellular responses to grazing effects in P. tricornutum and extends the understanding of co-evolutionary roles in regulating grazing defence between P. tricornutum and its grazer.
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Butler, Thomas, Rahul Vijay Kapoore, and Seetharaman Vaidyanathan. "Phaeodactylum tricornutum: A Diatom Cell Factory." Trends in Biotechnology 38, no. 6 (June 2020): 606–22. http://dx.doi.org/10.1016/j.tibtech.2019.12.023.

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Schmid-Staiger, U., C. Groeger, A. Seibert, U. Schliessmann, and T. Hirth. "Untersuchung des Zellaufschlusses von Phaeodactylum tricornutum." Chemie Ingenieur Technik 84, no. 8 (July 25, 2012): 1179. http://dx.doi.org/10.1002/cite.201250416.

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Zhang, Wenlei, Wenjun Zhou, Si Jiang, Yanyan Wang, Lin Chen, Guanpin Yang, and Tianzhong Liu. "Heterotrophic modification of Phaeodactylum tricornutum Bohlin." Algal Research 72 (May 2023): 103137. http://dx.doi.org/10.1016/j.algal.2023.103137.

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Zhang, Sheng-Hui, Juan Yu, Qiong-Yao Ding, Gui-Peng Yang, Kun-Shan Gao, Hong-Hai Zhang, and Da-Wei Pan. "Effect of elevated <i>p</i>CO<sub>2</sub> on trace gas production during an ocean acidification mesocosm experiment." Biogeosciences 15, no. 21 (November 9, 2018): 6649–58. http://dx.doi.org/10.5194/bg-15-6649-2018.

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Abstract. A mesocosm experiment was conducted in Wuyuan Bay (Xiamen), China, to investigate the effects of elevated pCO2 on the phytoplankton species Phaeodactylum tricornutum (P. tricornutum), Thalassiosira weissflogii (T. weissflogii) and Emiliania huxleyi (E. huxleyi) and their production ability of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), as well as four halocarbon compounds, bromodichloromethane (CHBrCl2), methyl bromide (CH3Br), dibromomethane (CH2Br2) and iodomethane (CH3I). Over a period of 5 weeks, P. tricornuntum outcompeted T. weissflogii and E. huxleyi, comprising more than 99 % of the final biomass. During the logarithmic growth phase (phase I), mean DMS concentration in high pCO2 mesocosms (1000 µatm) was 28 % lower than that in low pCO2 mesocosms (400 µatm). Elevated pCO2 led to a delay in DMSP-consuming bacteria concentrations attached to T. weissflogii and P. tricornutum and finally resulted in the delay of DMS concentration in the high pCO2 treatment. Unlike DMS, the elevated pCO2 did not affect DMSP production ability of T. weissflogii or P. tricornuntum throughout the 5-week culture. A positive relationship was detected between CH3I and T. weissflogii and P. tricornuntum during the experiment, and there was a 40 % reduction in mean CH3I concentration in the high pCO2 mesocosms. CHBrCl2, CH3Br, and CH2Br2 concentrations did not increase with elevated chlorophyll a (Chl a) concentrations compared with DMS(P) and CH3I, and there were no major peaks both in the high pCO2 or low pCO2 mesocosms. In addition, no effect of elevated pCO2 was identified for any of the three bromocarbons.
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Dissertations / Theses on the topic "Phaeodactylum tricornutum"

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Lin, Xin. "Charaterization of the Phaeodactylum tricornutum epigenome." Thesis, Paris 11, 2012. http://www.theses.fr/2012PA112235/document.

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La méthylation de l'ADN est l’une des marques épigénétiques les plus étudiées et est largement conservée. Mes travaux de thèse présentent le premier méthylome d'une diatomée marine P. tricornutum qui appartient à la famille des Stramenopiles. P. tricornutum présente une méthylation d’environ 6% qui est présente en mosaïque sur l’ensemble du génome. Une méthylation importante a été retrouvé chez les éléments transposables, en particulier les éléments amplifiés récemment de type Copia. L’analyse met en évidence plus de 320 gènes méthylés dans trois contextes génomiques différents : à proximité des éléments transposables, en grappes de gènes méthylés, et dans des gènes uniques. En outre, les gènes largement et complètement méthylés ont été trouvé fortement corrélés avec le silencing transcriptionnel et l'expression différentielle dans des conditions spécifiques. Enfin, il a été constaté que les gènes susceptibles d’avoir été acquis par transfert horizontal de gènes bactériens étaient préférentiellement insérés dans des régions riches en éléments transposables, ce qui suggère un mécanisme par lequel l'expression de gènes étrangers peut être tamponnée à la suite de leur insertion dans le génome. En résumé, P. tricornutum a une faible méthylation de l'ADN et une methylation relativement importante des éléments transposables et seulement quelques gènes méthylés. Ce premier méthylome d’une diatomée Stramenopile ajoute de manière significative à notre compréhension de l'évolution de la méthylation de l'ADN chez les eucaryotes. En ce qui concerne les modifications des histones, la distribution des marques H3K4me2, H3K9me2 et H3K27me3 a été examinée chez P. tricornutum. H3K4me2 est principalement associée à des gènes alors que les deux marques H3K9me2 et H3K27me3 ciblent principalement des éléments transposables. La répartition de H3K27me3 est inhabituelle et différente de ce qui a été observé chez les espèces modèles étudiées à ce jour. Les gènes marqués par H3K27me3 ont tendance à être faiblement exprimés et de façon différentielle. H3K27me3 et H3K9me2 ont tendance à co-marquer non seulement les éléments transposables méthylés, mais aussi des gènes fortement méthylés, ce qui semble être important pour le maintien du silencing des gènes différentiellement exprimés. L'analyse combinatoire de différentes marques d’histones et la méthylation de l'ADN nous a donné un aperçu du paysage de la chromatine chez les diatomées, et aidera à définir les caractéristiques structurales et fonctionnelles conservées
DNA methylation is the most extensively studied and widely conserved epigenetic mark. Here the first whole genome methylome from a stramenopile, the marine model diatom P. tricornutum is reported. In P. tricornutum, around 6% of the genome was methylated in a mosaic landscape. Extensive methylation in transposable elements (TEs), especially in recently amplified Copia-like elements was found. Over 320 genes were found methylated occurring in three different genomic contexts: in the proximity of TEs, in clusters of methylated genes, and in single genes. Furthermore, genes extensively and completely methylated correlated strongly with transcriptional silencing and differential expression under specific conditions. Finally, it was found that genes likely acquired by horizontal gene transfer from bacteria were preferentially inserted within TE-rich regions, suggesting a mechanism whereby the expression of foreign genes can be buffered following their insertion in the genome. In general, P. tricornutum has low DNA methylation with relatively extensive DNA methylation on TEs and a few methylated genes. This first Stramenopile methylome adds significantly to our understanding of the evolution of DNA methylation in eukaryotes. As for the histone modifications, genome wide distribution of H3K4me2, H3K9me2 and H3K27me3 were examined in P. tricornutum. H3K4me2 is mainly associated with genes while both H3K9me2 and H3K27me3 marks target mainly transposable elements (TEs). The distribution of H3K27me3 is unusual and different from what have been profiled in model species so far. The genes marked by H3K27me3 tend to be lowly and differentially expressed. H3K27me3 and H3K9me2 tend to co-mark not only methylated TEs but also heavily methylated genes, which appears to be important for maintaining the silencing of differentially expressed genes. The combinatorial analysis of different histone marks and DNA methylation gave us an overview of diatom chromatin landscapes, and will help to define conserved structural and functional features
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Singh, Dipali. "Genome scale metabolic modelling of Phaeodactylum tricornutum." Thesis, Oxford Brookes University, 2017. https://radar.brookes.ac.uk/radar/items/25847ffb-0605-4572-9165-a07c849f83c7/1/.

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Diatoms are photoautotrophic unicellular algae and are among the most abundant, adaptable and diverse marine phytoplankton. Their ability to synthesise lipid as a storage compound (20%-50% dry cell weight) makes them a potential sources of biofuel and high-value commodities such as ω fatty acids. However, diatoms have unique features in their biochemistry as compared to higher plants and hence, there is a prior need to understand diatom metabolism to enable physiological and genetic manipulation, and improve their strains. The present work involves construction and analysis of genome scale metabolic models (GSMs) of Phaeodactylum tricornutum, a model diatom, the characterisation of physiological properties and the identification of the potential strategies to optimise the lipid production. GSMs were constructed based on a previously published model and metabolic databases, and were analysed using structural modelling techniques to understand the metabolic responses at different environmental and physiological conditions. The model results suggest change in metabolic responses, mainly associated with the Calvin cycle, reductant transfer, photorespiration, TCA cycle, glyoxylate cycle, lipid metabolism, carbohydrate metabolism and energy dissipation mechanisms under changing environmental and physiological conditions. Carbon xation and triose-phosphate production can take place solely in the chloroplast, despite of differences in the localisation and regulation of the Calvin cycle enzymes as compared to higher plants. Further, model analysis suggests that lipid production in P. tricornutum increases both when exposed to high light and with the availability of glycerol. The potential metabolic routes for lipid production involves phosphoketolase pathway, threonine metabolism, recycling of glycolate and HCO3 fixation. Based on the model analysis, experiments were designed where cultures were exposed to high light, supplemented with HCO3 , under phototrophic and mixotrophic conditions. This resulted to an increase in biomass and lipid productivity. In addition, by revealing the potential metabolic routes involved with lipid production, our work also suggests possible targets for metabolic engineering that could divert carbon towards lipid production.
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Figueiredo, Bruna Flávia Henriques Tavares. "Carotenoids extraction from the microalga Phaeodactylum tricornutum." Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/19136.

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Mestrado em Biotecnologia, ramo de Biotecnologia Industrial e Ambiental
Microalgae have been attracting a crescent attention regarding either the potential use of their biomass or the wide range of compounds of interest that they have in their constitution, which can be extracted and applied in several fields of industry, namely carotenoids and in particular fucoxanthin.. Fucoxanthin is a photosynthetic pigment, with numerous applications and benefits, namely in the human healthrelated domains, due to its high antioxidant properties, essential in the prevention and treatment of several diseases, such as cancer and cardiovascular diseases. Hereupon, the development of a feasible route for the production of carotenoids rich in fucoxanthin which could allow its commercialization is of utmost scientific, industrial and even social relevance. The Phaeodactylum tricornutum species is being reported as a relevant producer of fucoxanthin. Still, there are no economic feasible extraction methods for the carotenoids, and in particular fucoxanthin, considering their efficient obtainment with high purity levels. Thus, this work is aiming at the development of a sustainable and feasible production and extraction platform for the extraction of carotenoids (and fucoxanthin) from Phaeodactylum tricornutum. In this sense, a conventional methodology was optimized, as well as a novel extraction methodology based on alternative tensioactive solvents, exclusively in aqueous solution, namely common surfactants, copolymers and tensioactive ionic liquids. Regarding the alternative method developed, it was possible to achieve a final extraction of 32.67 mgcarotenoids/gbiomass with the ionic liquid [C18mim]Cl and 10.69 mgfucoxanthin/gbiomass with [C14mim]Cl, similar values or even superior when compared with those found for the conventional methodology. At the end, the achievement of fucoxanthin in its purest form is foreseen for further industrial application.
As microalgas têm vindo a despertar cada vez mais interesse pelas potencialidades de utilização da biomassa como um todo, quer pela diversidade de compostos de interesse que as constituem, com aplicações variadas, como é o caso dos carotenóides e, em particular, da fucoxantina. Fucoxantina é um pigmento fotossintético com inúmeras aplicações e benefícios, nomeadamente na área da saúde, pelasua elevada atividade antioxidante, importante na prevenção e tratamento de várias doenças, como o cancro e doenças cardiovasculares. Desta forma, é de interesse científico, industrial e mesmo social, o desenvolvimento de uma plataforma economicamente eficiente de produção e extração destes carotenóides que permitam o seu uso em larga escala, visando assim a sua aplicação industrial. Existem diversos estudos que apontam as microalgas como grandes produtoras deste pigmento, nomeadamente a espécie Phaeodactylum tricornutum. No entanto, os métodos de extração e purificação de carotenóides e em particular de fucoxantina que permitam a recuperação destes compostos com elevado grau de pureza são ainda algo deficitários, no que diz respeito ao seu elevado impacto económico e ambiental. Assim, o objetivo deste trabalho recai no desenvolvimento de uma plataforma rentável para produção e extração de carotenóides (e fucoxantina) a partir da microalga Phaeodactylum tricornutum. Para tal, o método de extração convencional foi otimizado, assim como foi desenvolvido um método de extração alternativo recorrendo ao uso de solventes alternativos com natureza tensioativa exclusivamente em solução aquosa, nomeadamente surfactantes comuns, copolímeros e líquidos iónicos tensioativos. A partir deste método alternativo foi possível extrair um total de 32.67 mgcarotenoides/gbiomassa com o [C18mim]Cl e 10.69 mgfucoxantina/gbiomassa com [C14mim]Cl, valores semelhantes ou mesmo superiores aos obtidos pelo método convencional.
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Kilian, Oliver. "Investigations on functional aspects of secondary endocytobiosis using the diatom Phaeodactylum tricornutum as a model organism." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=973266724.

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Dolch, Lina-Juana. "Glycerolipid metabolism and regulation in Phaeodactylum tricornutum and Nannochloropsis gaditana." Thesis, Université Grenoble Alpes (ComUE), 2016. http://www.theses.fr/2016GREAV026/document.

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Phaeodactylum et Nannochloropsis sont des espèces photosynthétiques modèles pour le métabolisme des glycérolipides, se distinguant par un enrichissement en acides gras polyinsaturés à très longues chaînes (VLC-PUFA) et de grandes quantités en triacylglycérol (TAG). Les proportions des différents lipides sont influencées par des facteurs environnementaux. Nous avons caractérisé le remodelage lipidique chez Phaeodactylum en réponse à la carence en azote et en phosphate. Ces limitations en nutriments induisent une accumulation de TAG, exploitable comme biocarburant. Nous avons identifié de nouveaux composés induisant l'accumulation de TAG et étudié le rôle potentiel du monoxyde d’azote (NO•) dans la régulation du métabolisme lipidique. Nous avons montré qu’en fonction du site de production, le NO• était un signal émis lorsque les conditions de vie étaient critiques, déclenchant l'accumulation de TAG.Les VLC-PUFAs sont produits par des élongases et des désaturases localisées dans le RE. Nous avons identifié une nouvelle classe d’élongases d’acides gras saturés, agissant sur le 16:0, et appelées Δ0-ELO. Le knock out de Δ0-ELO1 de Nannochloropsis réduit le niveau du monogalactosyldiacylglycérol (MGDG), principal lipide des chloroplastes. Ce phénotype met en évidence le rôle de Δ0-ELO1 dans la «voie oméga» qui contrôle le trafic des VLC-PUFAs. Nous avons débuté une dissection de la «voie oméga» par des approches de génétique et des analyses du remodelage lipidique à basse température chez Nannochloropsis. Le diacylglycéryl hydroxyméthyltriméthyl-β-sérine (DGTS) apparaît comme le précurseur de base pour importer des VLC-PUFAs vers le chloroplaste, suivant une voie très régulée du DGTS au MGDG. De plus nous avons montré des fonctions possibles du MGDG et des VLC-PUFAs dans la photoprotection et la régulation de la fluidité membranaire latérale
Phaeodactylum and Nannochloropsis are photosynthetic model species for glycerolipid metabolism, standing out by an enrichment of very-long-chain polyunsaturated fatty acids (VLC-PUFAs) and high contents of neutral lipids such as triacylglycerol (TAG). Lipid profiles are influenced by environmental factors. We characterized the lipid remodelling occurring in Phaeodactylum in response to nitrogen and phosphate starvation. Nutrient limitations induce neutral lipid accumulation, which may be exploited as biofuels. We identified new triggers of TAG accumulation and investigated a potential role of nitric oxide (NO•) as second messenger in the regulation of neutral lipid levels. We conclude that in dependence of the production site, NO• serves as a signalling molecule for critical life conditions and thereby triggers TAG accumulation.VLC-PUFAs are produced by ER-located elongases and desaturases. We identified a novel class of elongases, called Δ0-ELOs, acting on saturated fatty acids, most importantly 16:0. Knock out of Δ0-ELO1 in Nannochloropsis resulted in reduced monogalactosyldiacylglycerol (MGDG) levels. MGDG is the major chloroplast lipid. This indicated a role of this initial elongase in fatty acid fate determination and thus in the elusive “omega pathway” for VLC-PUFA trafficking. We have started to investigate the “omega pathway” by reverse genetic approaches and analyses of low-temperature induced lipid remodelling in Nannochloropsis. Diacylglyceryl hydroxymethyltrimethyl-β-serine (DGTS) appears most likely at the base for the chloroplast import of VLC-PUFA, following a dynamically regulated DGTS-to-MGDG pathway. Additionally, we gave insights into possible functions of MGDG and VLC-PUFA in photoprotection and regulation of membrane fluidity
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Materna, Arne Christian. "Development of molecular tools in the diatom Phaeodactylum tricornutum." [S.l. : s.n.], 2006. http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-52997.

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Villanova, Valeria. "Identification du mécanisme de la mixotrophie chez Phaeodactylum tricornutum." Thesis, Université Grenoble Alpes (ComUE), 2016. http://www.theses.fr/2016GREAV053/document.

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Les diatomées jouent un rôle primordial dans l'écologie de la planète, car elles sont responsables du 20-40% de la productivite mondial d’oxygène. Elles figurent parmi les organismes à fort potentiel biotechnologique pour des applications biocarburant. Les diatomées sont des organismes symbiotiques issus de la fusion d'un ancêtre hétérotrophe avec une ou plusieurs micro-algues photosynthétiques. Grace à cette histoire évolutive complexe, les diatomées ont un métabolisme très flexible. Comme la plus part des microalgues elles peuvent utiliser la photosynthèse pour leur croissance, mais aussi la mixotrophie, i.e. la capacité de croître en présence de lumière et d’une source de carbone réduit. L'utilisation simultanée de la photosynthèse et de la respiration peut augmenter la productivité de la biomasse des microalgues et réduire ainsi le coût de leur exploitation industrielle. Dans cette thèse j’ai étudié le mécanisme et les conséquences du métabolisme mixotrophique chez la diatomée modèle Phaeodactylum tricornutum. J’ai contribué à étudier le mécanisme moléculaire à la base des interactions énérgétiques entre chloroplaste et mitochondrie. Dans ce travail, nous avons démontré que le NADPH généré dans le chloroplaste est exporté vers la mitochondrie pour générer de l’ATP requis pour la fixation du CO2 dans le chloroplaste. Cette interaction entre les deux organites cellulaires augmente la croissance de diatomées, et suggère que l'utilisation simultanée d’une source de carbone et de l'énergie lumineuse (mixotrophie) devrait augmenter la productivité de la biomasse chez les diatomées. Cette hypothèse a été testée dans la deuxième partie de ma thèse, où j’ai etudié les conséquences de la mixotrophie sur le métabolisme de Phaeodactylum. Grace à une approche métabolomique, transcriptomique, lipidomiques et de physiologie j’ai contribué à éclaircir les principales voies métaboliques (métabolisme centrale, métabolisme des lipides, métabolisme des polymères de réserve) concerné la mixotrophie. Dans la dernière partie de ce travail j’ai optimisé les conditions de culture et la composition du milieu afin d’améliorer la productivité en croissance mixotrophe chez Phaeodactylum. Ce résultat a été validé dans des photobioréacteurs à l'échelle labo pour tester le potentiel de l'exploitation industrielle de cet organisme
Diatoms are photosynthetic organisms with a strong influence on the global biogeochemistry. Moreover, they are extremely interesting as potential feedstocks for the production of high-value molecules and biofuel. They are endosymbiotic organisms originated by the fusion of a heterotrophic ancestor with one or more photosynthetic microalgae. This has led to an extremely flexible cell metabolism. Like other microalgae, diatoms are able to grow in the presence of both light and of a reduced carbon source. The simultaneous use of photosynthesis and respiration can increase biomass productivity and reduce the energy cost of the industrial exploitation of diatoms.In this project, the mechanism and the consequences of mixotrophic metabolism have been studied in the model diatom Phaeodactylum tricornutum. In the first part, I have studied the molecular mechanism governing the interactions between chloroplast and mitochondrion. We have demonstrated that the NADPH generated in the plastid is exported to the mitochondrion to generate additional ATP, which, once back to the plastid, is used for carbon fixation. Overall, this work shows that the interaction between these two organelles increases carbon fixation and growth in diatoms. We hence suggest that the simultaneous use of carbon and light energy sources (i.e. mixotrophy) should enhance biomass productivity in diatoms. This hypothesis has been tested in the second part of my thesis, where I focused on the consequences of mixotrophy on metabolism. By combining metabolomic, transcriptomic, lipidomic and physiology approaches, I have contributed to elucidate the main pathways targeted by mixotrophy (central carbon, lipid and storage carbon metabolism). In the last part of this work, I have worked on improving the culture conditions and medium composition to boost microalgal productivity by mixotrophy. These conditions have been scaled-up in lab scale photobioreactors, revealing the industrial exploitation potential of Phaeodactylum
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Rastogi, Achal. "Phaeodactylum tricornutum genome and epigenome : characterization of natural variants." Thesis, Paris Sciences et Lettres (ComUE), 2016. http://www.theses.fr/2016PSLEE048/document.

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Depuis la découverte de Phaeodactylum tricornutum par Bohlin en 1897, sa classification au sein de l'arbre de la vie a été controversée. En utilisant des morphotypes ovales et fusiformes Lewin a décrit en 1958 plusieurs traits caractéristiques de cette espèce rappelant la structure des diatomées mettant ainsi fin à la controverse sur la classification de P. tricornutum au sein des Bacillariophycées. Pour se faire, trois morphotypes (ovale, fusiforme et triradié) de Phaeodactylum tricornutum ont été observés. Au cours d’une centaine d’années environ, de 1908 à 2000, 10 souches de Phaeodactylum tricornutum (appelées écotypes) ont été collectées et stockées soit de manière axénique ou en l’état avec leur populations naturelles de bactéries dans les centres des ressources génétiques pour algues, cryo-préservées quand cela est possible. Divers outils cellulaires et moléculaires ont été établis pour disséquer et comprendre la physiologie et l'évolution de P. tricornutum, et/ou les diatomées en général. Grâce à des décennies de recherche et les efforts déployés par de nombreux laboratoires que P. tricornutum est aujourd’hui considérée comme une espèce modèle des diatomées. Le sujet de ma thèse traite majoritairement de la composition génétique et épigénétique du génome de P. tricornutum ainsi que de la diversité morphologique et physiologique sousjacente au sein des populations naturelles prospectées à différents endroits du globe. Pour se faire, j’ai généré les profils chromatiniens en utilisant différentes marques des modifications post-traductionnelles des histones (chapitres 1 et 2) et a également comparé la variation naturelle dans la distribution de certaines marques clés entre deux populations d’écotypes (chapitre 4). Nous avons également généré une carte de la diversité génétique à l’échelle du génome chez 10 écotypes de P. tricornutum révélant ainsi la présence d'un complexe d'espèces dans le genre Phaeodactylum comme la conséquence d’une hybridation ancienne (chapitre 3). Sur la base de nombreux rapports antérieurs et des observations similaires au sein de P. tricornutum, nous proposons l’hybridation naturelle comme une base solide et une possibilité plausible pour expliquer la diversité des espèces chez lest diatomées. De plus, nous avons mis à jour les annotations fonctionnelles et structurelles du génome de P. tricornutum (Phatr3, chapitre 2) et mis au point un algorithme de logiciel convivial pour aller chercher les cibles CRISPR du système d’édition du génome CRISPR / cas9 chez 13 génomes de phytoplancton incluant P. tricornutum (chapitre 5). Pour accomplir tout cela, j'ai utilisé diverses méthodes à la pointe de l’état de l’art comme la spectrométrie de masse, l’immunoprécipitation de la chromatine suivie de séquençage à haut débit ainsi que les séquençages du génome entier, de l'ARN et des protocoles d'édition du génome CRISPR et plusieurs logiciels / pipelines de calcul. Ainsi, le travail de thèse fournit une plate-forme complète qui pourra être utilisée à l’avenir pour des études épigénétiques, de génétiques moléculaires et fonctionnelles chez les diatomées en utilisant comme espèce modèle Phaeodactylum tricornutum. Ce travail est pionnier et représente une valeur ajoutée importante dans le domaine de la recherche sur les diatomées en répondant à des questions nouvelles ouvrant ainsi de nouveaux horizons à la recherche en particulier en épigénétique qui joue un rôle important mais pas encore assez apprécié dans le succès écologique des diatomées dans les océans actuels
Since the discovery of Phaeodactylum tricornutum by Bohlin in 1897, its classification within the tree of life has been controversial. It was in 1958 when Lewin, using oval and fusiform morphotypes, described multiple characteristic features of this species that resemble diatoms structure, the debate to whether classify P. tricornutum as a member of Bacillariophyceae was ended. To this point three morphotypes (oval, fusiform and triradiate) of Phaeodactylum tricornutum have been observed. Over the course of approximately 100 years, from 1908 till 2000, 10 strains of Phaeodactylum tricornutum (referred to asecotypes) have been collected and stored axenically as cryopreserved stocks at various stock centers. Various cellular and molecular tools have been established to dissect and understand the physiology and evolution of P. tricornutum, and/or diatoms in general. It is because of decades of research and efforts by many laboratories that now P. tricornutum is considered to be a model diatom species. My thesis majorly focuses in understanding the genetic and epigenetic makeup of P. tricornutum genome to decipher the underlying morphological and physiological diversity within different ecotype populations. To do so, I established the epigenetic landscape within P. tricornutum genome using various histone post-translational modification marks (chapter 1 and chapter 2) and also compared the natural variation in the distribution of some key histone PTMs between two ecotype populations (chapter 4). We also generated a genome-wide genetic diversity map across 10 ecotypes of P. tricornutum revealing the presence of a species-complex within the genus Phaeodactylum as aconsequence of ancient hybridization (Chapter 3). Based on the evidences from many previous reports and similar observations within P. tricornutum, we propose natural hybridization as a strong and potential foundation for explaining unprecedented species diversity within the diatom clade. Moreover, we updated the functional and structural annotations of P. tricornutum genome (Phatr3, chapter 2) and developed a user-friendly software algorithm to fetch CRISPR/Cas9 targets, which is a basis to perform knockout studies using CRISPR/Cas9 genome editing protocol, in 13 phytoplankton genomes including P. tricornutum (chapter 5). To accomplish all this, I used various state-of-the-art technologies like Mass-Spectrometry, ChIPsequencing, Whole genome sequencing, RNA sequencing, CRISPR genome editing protocols and several computational softwares/pipelines. In brief, the thesis work provides a comprehensive platform for future epigenetic, genetic and functional molecular studies in diatoms using Phaeodactylum tricornutum as a model. The work is an addon value to the current state of diatom research by answering questions that have never been asked before and opens a completely new horizon and demand of epigenetics research that underlie the ecological success of diatoms in modern-day ocean
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Rastogi, Achal. "Phaeodactylum tricornutum genome and epigenome : characterization of natural variants." Electronic Thesis or Diss., Paris Sciences et Lettres (ComUE), 2016. http://www.theses.fr/2016PSLEE048.

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Depuis la découverte de Phaeodactylum tricornutum par Bohlin en 1897, sa classification au sein de l'arbre de la vie a été controversée. En utilisant des morphotypes ovales et fusiformes Lewin a décrit en 1958 plusieurs traits caractéristiques de cette espèce rappelant la structure des diatomées mettant ainsi fin à la controverse sur la classification de P. tricornutum au sein des Bacillariophycées. Pour se faire, trois morphotypes (ovale, fusiforme et triradié) de Phaeodactylum tricornutum ont été observés. Au cours d’une centaine d’années environ, de 1908 à 2000, 10 souches de Phaeodactylum tricornutum (appelées écotypes) ont été collectées et stockées soit de manière axénique ou en l’état avec leur populations naturelles de bactéries dans les centres des ressources génétiques pour algues, cryo-préservées quand cela est possible. Divers outils cellulaires et moléculaires ont été établis pour disséquer et comprendre la physiologie et l'évolution de P. tricornutum, et/ou les diatomées en général. Grâce à des décennies de recherche et les efforts déployés par de nombreux laboratoires que P. tricornutum est aujourd’hui considérée comme une espèce modèle des diatomées. Le sujet de ma thèse traite majoritairement de la composition génétique et épigénétique du génome de P. tricornutum ainsi que de la diversité morphologique et physiologique sousjacente au sein des populations naturelles prospectées à différents endroits du globe. Pour se faire, j’ai généré les profils chromatiniens en utilisant différentes marques des modifications post-traductionnelles des histones (chapitres 1 et 2) et a également comparé la variation naturelle dans la distribution de certaines marques clés entre deux populations d’écotypes (chapitre 4). Nous avons également généré une carte de la diversité génétique à l’échelle du génome chez 10 écotypes de P. tricornutum révélant ainsi la présence d'un complexe d'espèces dans le genre Phaeodactylum comme la conséquence d’une hybridation ancienne (chapitre 3). Sur la base de nombreux rapports antérieurs et des observations similaires au sein de P. tricornutum, nous proposons l’hybridation naturelle comme une base solide et une possibilité plausible pour expliquer la diversité des espèces chez lest diatomées. De plus, nous avons mis à jour les annotations fonctionnelles et structurelles du génome de P. tricornutum (Phatr3, chapitre 2) et mis au point un algorithme de logiciel convivial pour aller chercher les cibles CRISPR du système d’édition du génome CRISPR / cas9 chez 13 génomes de phytoplancton incluant P. tricornutum (chapitre 5). Pour accomplir tout cela, j'ai utilisé diverses méthodes à la pointe de l’état de l’art comme la spectrométrie de masse, l’immunoprécipitation de la chromatine suivie de séquençage à haut débit ainsi que les séquençages du génome entier, de l'ARN et des protocoles d'édition du génome CRISPR et plusieurs logiciels / pipelines de calcul. Ainsi, le travail de thèse fournit une plate-forme complète qui pourra être utilisée à l’avenir pour des études épigénétiques, de génétiques moléculaires et fonctionnelles chez les diatomées en utilisant comme espèce modèle Phaeodactylum tricornutum. Ce travail est pionnier et représente une valeur ajoutée importante dans le domaine de la recherche sur les diatomées en répondant à des questions nouvelles ouvrant ainsi de nouveaux horizons à la recherche en particulier en épigénétique qui joue un rôle important mais pas encore assez apprécié dans le succès écologique des diatomées dans les océans actuels
Since the discovery of Phaeodactylum tricornutum by Bohlin in 1897, its classification within the tree of life has been controversial. It was in 1958 when Lewin, using oval and fusiform morphotypes, described multiple characteristic features of this species that resemble diatoms structure, the debate to whether classify P. tricornutum as a member of Bacillariophyceae was ended. To this point three morphotypes (oval, fusiform and triradiate) of Phaeodactylum tricornutum have been observed. Over the course of approximately 100 years, from 1908 till 2000, 10 strains of Phaeodactylum tricornutum (referred to asecotypes) have been collected and stored axenically as cryopreserved stocks at various stock centers. Various cellular and molecular tools have been established to dissect and understand the physiology and evolution of P. tricornutum, and/or diatoms in general. It is because of decades of research and efforts by many laboratories that now P. tricornutum is considered to be a model diatom species. My thesis majorly focuses in understanding the genetic and epigenetic makeup of P. tricornutum genome to decipher the underlying morphological and physiological diversity within different ecotype populations. To do so, I established the epigenetic landscape within P. tricornutum genome using various histone post-translational modification marks (chapter 1 and chapter 2) and also compared the natural variation in the distribution of some key histone PTMs between two ecotype populations (chapter 4). We also generated a genome-wide genetic diversity map across 10 ecotypes of P. tricornutum revealing the presence of a species-complex within the genus Phaeodactylum as aconsequence of ancient hybridization (Chapter 3). Based on the evidences from many previous reports and similar observations within P. tricornutum, we propose natural hybridization as a strong and potential foundation for explaining unprecedented species diversity within the diatom clade. Moreover, we updated the functional and structural annotations of P. tricornutum genome (Phatr3, chapter 2) and developed a user-friendly software algorithm to fetch CRISPR/Cas9 targets, which is a basis to perform knockout studies using CRISPR/Cas9 genome editing protocol, in 13 phytoplankton genomes including P. tricornutum (chapter 5). To accomplish all this, I used various state-of-the-art technologies like Mass-Spectrometry, ChIPsequencing, Whole genome sequencing, RNA sequencing, CRISPR genome editing protocols and several computational softwares/pipelines. In brief, the thesis work provides a comprehensive platform for future epigenetic, genetic and functional molecular studies in diatoms using Phaeodactylum tricornutum as a model. The work is an addon value to the current state of diatom research by answering questions that have never been asked before and opens a completely new horizon and demand of epigenetics research that underlie the ecological success of diatoms in modern-day ocean
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Gaeta, Salvador Airton. "COMPARACAO DAS RESPOSTAS DE CRESCIMENTO E FOTOSSINTESE DE TRES CLONES DE PHAEODACTYLUM TRICORNUTUM." Universidade de São Paulo, 1985. http://www.teses.usp.br/teses/disponiveis/21/21131/tde-16062010-145335/.

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Através de bioensaios estáticos fo-ram comparadas as taxas máximas de crescimento, razão de fotossintese em termos de clorofila-a e de célula, bem como o rendimento final de tres clones de Phaeodacty-tum tricornutum visando verificar uma possível existência de diferenças intraespe-cificas entre eles. Dois desses clones foram isolados a partir de amostras de Uba-tuba (U3, isolado em 1972 e U5 isolado em 1979) e um de Cananéia (Cl, isolado em 1980). Todos os resultados foram submeti-dos a uma análise de variância trifatorial e a uma anállse de agrupamento com a finlidade de discriminar as condições experimentais nas quais os tres clones diferi-ram significativamente em suas respostas fisiológicas. Durante a fase exponencial de crescimento os tres clones se apresen-taram com morfótipos fusiformes raramente se encontrando morfótipos ovóides e trir-radiados. O padrão das curvas de cresci -mento dos tres clones em diferentes temperaturas (16, 20, 240 C) e meios de cultu-ra (ASP 2, Erd-Schreiber, Guillard fl) não diferiu. Os resultados obtidos indicaram a existência de diferenças significativas nas taxas de crescimento e produti-vidade primária. Morfótipo ovóides for-mando grumos foram observados nos tres clones após a fase exponencial e de modo conspícuo no clone U5. Nas mesmas condi-ções, o clone Cl apresentou uma grande quantidade de grumos, com a forma de uma estrela, constituidos por morfótipos fusiformes unidos convergentemente por uma das extremidades de cada célula. Por outro lado, morfótipos trirradiados e cru-ciformes bem como formação de cadeias, só foram observados no clone U3.
A comparison of the maximum growth rates, primary productivity on chlorophyll -a and cell basis, and maximum yield achieved in batch culture of three clones of Phaeodactylum tricornutum has been carried out in order to cheek intraespecifie differences among them. Two of the clones were isolated from samples of Uba-tuba (U3, isolated in 1972 and U5, in 1979 ), while the third one was isolated from samples of Cananéia (C1, isolated in 1980). Three-way analysis of variance was accom-plished and cluster analysis was carried out to distinguish the experimental conditions in which the clones would show distinct physiological responses. During the log phase of growth the three clones usually presented fusiform type of cells. Never theless, rare oval and triradiate cells have been observed. The pattern of the growth curves submitted todifferent temperatures (16,20,24º C) and media (Guillard f1. Erd-Schreiber, ASP2) was the same for the three clones. The results indieated significant differenees in growth rates and primary productivity. Oval morphotypes in clusters have been observed in all clones after the log phase under exausted media. The clone U5 has exhibited this be haviour in a prominent way. Many star-shape clusters, constituted of fusiform morphotypes linked by one of the extremities of each cell were observed in clone Cl. Un the other hand, triradiate and cruciform cells, and the formation of chains, were observed only in clone U3.
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Books on the topic "Phaeodactylum tricornutum"

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Heywood, Steven D. Effects of ultraviolet-B radiation on the biochemical composition of a marine diatom, Phaeodactylum tricornutum. 1997.

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Book chapters on the topic "Phaeodactylum tricornutum"

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Hu, Hanhua, and Yufang Pan. "Electroporation Transformation Protocol for Phaeodactylum tricornutum." In Methods in Molecular Biology, 163–67. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9740-4_17.

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Domergue, F., T. K. Zank, A. Abbadi, P. Sperling, A. Meyer, J. Lerchl, and E. Heinz. "Fatty Acid Desaturases from the Diatom Phaeodactylum Tricornutum." In Advanced Research on Plant Lipids, 121–24. Dordrecht: Springer Netherlands, 2003. http://dx.doi.org/10.1007/978-94-017-0159-4_27.

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Popko, Jennifer. "Lipid Composition of the Model Diatom Phaeodactylum tricornutum." In Encyclopedia of Lipidomics, 1–7. Dordrecht: Springer Netherlands, 2016. http://dx.doi.org/10.1007/978-94-007-7864-1_127-1.

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Ifuku, Kentaro, and Dongyi Yan. "Efficient Transformation of the Diatoms Phaeodactylum tricornutum by Multipulse Electroporation." In Methods in Molecular Biology, 169–74. New York, NY: Springer US, 2019. http://dx.doi.org/10.1007/978-1-4939-9740-4_18.

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Hu, Fan, Wenxiu Yin, Teng Huang, and Hanhua Hu. "Isolation of High-Quality Plastids from the Diatom Phaeodactylum tricornutum." In Methods in Molecular Biology, 177–83. New York, NY: Springer US, 2024. http://dx.doi.org/10.1007/978-1-0716-3726-5_10.

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Bauer, Claudia M., Paulo Vilaça, Fernanda Ramlov, Eva Regina de Oliveira, Débora Q. Cabral, Caroline Schmitz, Rafaela Gordo Corrêa, Miguel Rocha, and Marcelo Maraschin. "In Silico Predictions for Fucoxanthin Production by the Diatom Phaeodactylum Tricornutum." In Practical Applications of Computational Biology and Bioinformatics, 12th International Conference, 139–48. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-98702-6_17.

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Geel, C., W. Groen-Versluis, and J. F. H. Snel. "Inhibition of Chlororespiration does not Affect the F0 in Phaeodactylum Tricornutum." In Photosynthesis: Mechanisms and Effects, 4329–32. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-3953-3_1001.

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Schober, Alexander F., Serena Flori, Giovanni Finazzi, Peter G. Kroth, and Carolina Río Bártulos. "Isolation of Plastid Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum." In Methods in Molecular Biology, 189–203. New York, NY: Springer US, 2018. http://dx.doi.org/10.1007/978-1-4939-8654-5_13.

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Manodori, Annamaria, and Arthur R. Grossman. "Sequence Homology Between Light Harvesting Polypeptides of Plants and the Diatom Phaeodactylum tricornutum." In Current Research in Photosynthesis, 2447–50. Dordrecht: Springer Netherlands, 1990. http://dx.doi.org/10.1007/978-94-009-0511-5_551.

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Mewes, Heiko, Michael Richter, Reimund Goss, and Christian Wilhelm. "Multiple Short Term Effects of UV-B Radiation on the Diatom Phaeodactylum Tricornutum." In Photosynthesis: Mechanisms and Effects, 2373–76. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-3953-3_557.

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Conference papers on the topic "Phaeodactylum tricornutum"

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Bedina Zavec, Apolonija, Darja Božič, Matej Hočevar, Aleš Iglič, Marko Jeran, Veronika Kralj Iglič, and Anna Romolo. "Scanning electron microscope images of Phaeodactylum tricornutum culture." In Socratic Lectures 9. University of Lubljana Press, 2024. http://dx.doi.org/10.55295/psl.2024.d12.

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Abstract:Scanning electron microscope images of small cellular particles isolated from conditioned media of microalgae Phaeodactylum tricornutum are presented Each image is supplemented by description of the preparation of the sample and the data on the imaging technique and equipment. Keywords: Extracellular vesicles, Extracellular particles, Nanoalgosomes, Exosomes
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Wishkerman, Asher, and Shoshana Arad (Malis). "Production of silver nanoparticles by the diatom Phaeodactylum tricornutum." In SPIE Microtechnologies, edited by Ion M. Tiginyanu. SPIE, 2017. http://dx.doi.org/10.1117/12.2264706.

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Listwan, Stanisław, Wiktor Tokarek, Krzysztof Kleszcz, Magdalena Chowaniec, Zofia Porębska, Katarzyna Krawczyk, Monika Bojko, and Dariusz Latowski. "Phaeodactylum Tricornutum as a Potential Phytoremediator of Sea and Fresh Waters." In The 4th World Congress on New Technologies. Avestia Publishing, 2018. http://dx.doi.org/10.11159/icepr18.147.

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Romolo, Anna, Matej Hočevar, Aleš Iglič, Tjaša Griessler Bulc, and Veronika Kralj-Iglič. "Short Term Effect of Plant Hybridosomes on Growth of Phaeodactylum Tricornutum Culture." In Socratic Lectures 8. University of Lubljana Press, 2023. http://dx.doi.org/10.55295/psl.2023.ii12.

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Microalgae are in focus of extensive study due to their abundance and important role in equilibration of the global ecosystem. Living organisms communicate through nano-sized membrane-enclosed particles which are continuously shed by cells but can also be fabri-cated artificially. In this work we examined the effect of hybridosomes composed from soyabean lecithin, aqueous solution containing substances from spruce needles and glyc-erol and hybridosomes composed from soyabean lecithin, ultraclean water and hemp oil on the number density of microalgae Phaeodactylum tricornutum in culture. We measured the number density of microalgae by flow cytometry and the number density and hydro-dynamic diameter of small particles in the samples by interferometric light microscopy. We observed considerable increase of the number density of microalgae with respect to control (untreated) samples after three days, which was connected to the amount of the material added. Addition of membrane-enclosed particles had a favourable effect on the microalgae growth. Microalgae proved a convenient system for in vitro studies of the ef-fects of substances. Keywords: Phaeodactylum tricornutum; Hybridosomes; Liposomes; Nanoalgosomes; Ex-tracellular vesicles; Small cellular particles; Microalgae
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Holden, Todd, P. Marchese, G. Tremberger, Jr., E. Cheung, R. Subramaniam, R. Sullivan, P. Schneider, et al. "Phaeodactylum tricornutum photosynthesis and Thalassiosira pseudonana bio-silica formation genes nucleotide fluctuations." In Optical Engineering + Applications, edited by Richard B. Hoover, Gilbert V. Levin, Alexei Y. Rozanov, and Paul C. Davies. SPIE, 2008. http://dx.doi.org/10.1117/12.794414.

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Bedina Zavec, Apolonija, Darja Božič, Aleš Iglič, Marko Jeran, Matic Kisovec, Veronika Kralj Iglič, and Anna Romolo. "C ryogenic transmission electron images of Phaeodactylum tricornutum isolates of small cellular particles." In Socratic Lectures 9. University of Lubljana Press, 2024. http://dx.doi.org/10.55295/psl.2024.d9.

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Abstract: Cryogenic transmission electron images of small cellular particles isolated from conditioned media of microalgae Phaeodactylum tricornutun are presented . Each image is identified by the name and number that constitutes the title of the Figure, and supplemented by description of the preparation of the sample and the data on the imaging technique and equipment. Keywords: Extracellular vesicles, Extracellular particles, Nanoalgosomes, Exosomes
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Bedina Zavec, Apolonija, Darja Božič, Matej Hočevar, Aleš Iglič, Marko Jeran, Veronika Kralj Iglič, and Anna Romolo. "Scanning electron microscope images of small cellular particles isolated from Phaeodactylum tricornutum conditioned media enriched with Guillard’s (F/2) and MW BG11 . Light and dark phases." In Socratic Lectures 9. University of Lubljana Press, 2024. http://dx.doi.org/10.55295/psl.2024.d14.

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Abstract : Scanning electron microscope images of small cellular particles isolated from Phaeodactylum tricornutun conditoned media , light and dark phases are presented . Each image is supplemented by description of the preparation of the sample and the data on the imaging technique and equipment. Keywords: Extracellular vesicles, Extracellular particles, Nanoalgosomes, Exosomes
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Warkiewicz, Edyta, Agnieszka Strączek, Dariusz Latowski, and Monika Bojko. "Acclimatization of Diatom Phaeodactylum Tricornutum to Long-Term Environmental Temperature and Light Intensity Changes." In The 4th World Congress on New Technologies. Avestia Publishing, 2018. http://dx.doi.org/10.11159/icepr18.146.

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Bedina Zavec, Apolonija, Darja Božič, Matej Hočevar, Aleš Iglič, Marko Jeran, Veronika Kralj Iglič, and Anna Romolo. "Scanning electron microscope images of isolates of small cellular particles Phaeodactylum tricornutum grown in media supplemented with Guillard’s (F/2) marine water enrichment solution, BG11 broth and Lennox LB broth." In Socratic Lectures 9. University of Lubljana Press, 2024. http://dx.doi.org/10.55295/psl.2024.d13.

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Abstract : Scanning electron microscope images of small cellular particles isolated from conditioned media of microalgae Phaeodactylum tricornutun are presented . Each image is supplemented by description of the preparation of the sample and the data on the imaging technique and equipment. Keywords: Extracellular vesicles, Extracellular particles, Nanoalgosomes, Exosomes
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Morais, K. C. C., J. V. C. Vargas, A. B. Mariano, J. C. Ordonez, and V. Kava. "Sustainable energy via biodiesel production from autotrophic and mixotrophic growth of the microalga Phaeodactylum tricornutum in compact photobioreactors." In 2016 IEEE Conference on Technologies for Sustainability (SusTech). IEEE, 2016. http://dx.doi.org/10.1109/sustech.2016.7897177.

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Reports on the topic "Phaeodactylum tricornutum"

1

Chorazyczewski, Adam M., Paul V. Zimba, Xavier Mayali, and Joe Fox. Do Phycosphere associated bacteria affect the growth and lipid production of Phaeodactylum tricornutum Bohlin? Office of Scientific and Technical Information (OSTI), February 2019. http://dx.doi.org/10.2172/1544511.

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