Journal articles: 'Phaeodactylum tricornutum'

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Bowler, Chris, and Angela Falciatore. "Phaeodactylum tricornutum." Trends in Genetics 35, no. 9 (September 2019): 706–7. http://dx.doi.org/10.1016/j.tig.2019.05.007.

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Veloso, V., A. Reis, L. Gouveia, H. L. Fernandes, J. A. Empis, and J. M. Novais. "Lipid production by Phaeodactylum tricornutum." Bioresource Technology 38, no. 2-3 (January 1991): 115–19. http://dx.doi.org/10.1016/0960-8524(91)90141-6.

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Sudo, Yoshitaka, Hajime Okawara, and Eiji O'Shima. "Growth Rate of Phaeodactylum tricornutum." KAGAKU KOGAKU RONBUNSHU 17, no. 3 (1991): 484–90. http://dx.doi.org/10.1252/kakoronbunshu.17.484.

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Burch, Andrew R., Cody W. Yothers, Michelle R. Salemi, Brett S. Phinney, Pramod Pandey, and Annaliese K. Franz. "Quantitative label-free proteomics and biochemical analysis of Phaeodactylum tricornutum cultivation on dairy manure wastewater." Journal of Applied Phycology 33, no. 4 (May 27, 2021): 2105–21. http://dx.doi.org/10.1007/s10811-021-02483-3.

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AbstractMicroalgae cultivation on wastewater offers the dual benefit of lowering costs for feedstock production with simultaneous wastewater remediation. This study utilized biochemical and quantitative label-free proteomic approaches to evaluate the growth and proteomic response for diatom Phaeodactylum tricornutum cultivated on flushed dairy manure wastewater (DMW). Comparing several DMW dilutions (up to 60% DMW diluted in seawater) with a synthetic seawater medium indicates that biomass and lipid yields correlate with the starting nitrogen content of the DMW dilution. Phaeodactylum tricornutum cultivated on DMW exhibits elevated levels of polyunsaturated fatty acids (PUFAs), particularly docosapentaenoic acid (DPA, 22:5 n-3). Proteomic analysis revealed alterations in the regulations of proteins associated with protein metabolism, cellular signaling, transcription and translation, protein trafficking, and oxidative stress management pathways when comparing P. tricornutum cultivation on diluted DMW versus synthetic media, thus providing insights into how P. tricornutum reorganizes its proteome in response to a complex wastewater source. Graphical abstract

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Ak, Burcu, Gökhan Tamer Kayaalp, Oya Işık, and Melis Çelik Güney. "Phaeodactylum Trıcornutum Kültürlerinde Biyomas Tahmini." Turkish Journal of Agriculture - Food Science and Technology 5, no. 2 (February 28, 2017): 182. http://dx.doi.org/10.24925/turjaf.v5i2.182-184.1145.

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Phaeodactylum tricornutum is the microalgae that is known to produce lipid. In this study, it was aimed to estimate the microalgae Phaeodactylum tricornutum dry matter cultured in the conditions of nitrogen deficiency as a stress factor, in outdoor, in photo bioreactors, by using multiple regression analysis method. In this study, diatom Phaeodactylum tricornutum (Bohlin) was cultured medium of which N was reduced by 50% and with 20% inoculation ratio. The light intensity, temperature, optical density and chlorophyll a, were measured daily. The mathematical model was formed for control group and 50 % of the N applied to the group using optical density, temperature, light intensity, chlorophyll a and without chlorophyll a. The regression equation of control group was estimated. The R2 value of control group was found 95.1% and statistically significant. Then, the regression equation was estimated for control group without chlorophyll a. The R2 value of this equation was found 94.0% and statistically significant. And then, the regression equation was estimated in 50% nitrogen. The R2 value of control group was found 92.4% and statistically significant. Finally, the regression equation was estimated in 50% nitrogen deficiency without chlorophyll a. The R2 value of this equation was found 91.7% and statistically significant.

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Li, Si, and Stefanie M. H. Ismar. "Transcriptome, Biochemical and Growth Responses of the Marine Phytoplankter Phaeodactylum Tricornutum Bohlin (Bacillariophyta) to Copepod Grazer Presence." Cellular Physiology and Biochemistry 46, no. 3 (2018): 1091–111. http://dx.doi.org/10.1159/000488839.

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Background/Aims: As a model organism for a pleiomorphic marine planktonic primary producer, Phaeodactylum tricornutum has been studied on a molecular level under diverse cultural conditions. But little is known about its morphological, nutritional or transcriptomic responses under grazing stress. Methods: To assess microalgal molecular and cellular responses to grazer presence, we conducted transcriptome profiling in combination with growth rate, biovolume, fatty acid content, carbon and nitrogen content measurements in the model diatom Phaeodactylum tricornutum. RNA-sequencing was used to evaluate the transcriptomic response to grazing stress for P. tricornutum strain CCAP 1055/1. Results: Among the differentially expressed genes, we found down-regulation of genes involved in pathogen resistance, and in fatty acid biosynthesis pathways, while mitosis-involved genes were up-regulated. Experimentally testing morphological and biochemical responses in five strains of the species, we detected strain-specific significant effects of simulated grazing pressure in altered growth rates, biovolume and nutritional composition. Conclusion: Our research reveals the associated molecular and cellular responses to grazing effects in P. tricornutum and extends the understanding of co-evolutionary roles in regulating grazing defence between P. tricornutum and its grazer.

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Butler, Thomas, Rahul Vijay Kapoore, and Seetharaman Vaidyanathan. "Phaeodactylum tricornutum: A Diatom Cell Factory." Trends in Biotechnology 38, no. 6 (June 2020): 606–22. http://dx.doi.org/10.1016/j.tibtech.2019.12.023.

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Schmid-Staiger, U., C. Groeger, A. Seibert, U. Schliessmann, and T. Hirth. "Untersuchung des Zellaufschlusses von Phaeodactylum tricornutum." Chemie Ingenieur Technik 84, no. 8 (July 25, 2012): 1179. http://dx.doi.org/10.1002/cite.201250416.

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Zhang, Wenlei, Wenjun Zhou, Si Jiang, Yanyan Wang, Lin Chen, Guanpin Yang, and Tianzhong Liu. "Heterotrophic modification of Phaeodactylum tricornutum Bohlin." Algal Research 72 (May 2023): 103137. http://dx.doi.org/10.1016/j.algal.2023.103137.

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Zhang, Sheng-Hui, Juan Yu, Qiong-Yao Ding, Gui-Peng Yang, Kun-Shan Gao, Hong-Hai Zhang, and Da-Wei Pan. "Effect of elevated <i>p</i>CO<sub>2</sub> on trace gas production during an ocean acidification mesocosm experiment." Biogeosciences 15, no. 21 (November 9, 2018): 6649–58. http://dx.doi.org/10.5194/bg-15-6649-2018.

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Abstract. A mesocosm experiment was conducted in Wuyuan Bay (Xiamen), China, to investigate the effects of elevated pCO2 on the phytoplankton species Phaeodactylum tricornutum (P. tricornutum), Thalassiosira weissflogii (T. weissflogii) and Emiliania huxleyi (E. huxleyi) and their production ability of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), as well as four halocarbon compounds, bromodichloromethane (CHBrCl2), methyl bromide (CH3Br), dibromomethane (CH2Br2) and iodomethane (CH3I). Over a period of 5 weeks, P. tricornuntum outcompeted T. weissflogii and E. huxleyi, comprising more than 99 % of the final biomass. During the logarithmic growth phase (phase I), mean DMS concentration in high pCO2 mesocosms (1000 µatm) was 28 % lower than that in low pCO2 mesocosms (400 µatm). Elevated pCO2 led to a delay in DMSP-consuming bacteria concentrations attached to T. weissflogii and P. tricornutum and finally resulted in the delay of DMS concentration in the high pCO2 treatment. Unlike DMS, the elevated pCO2 did not affect DMSP production ability of T. weissflogii or P. tricornuntum throughout the 5-week culture. A positive relationship was detected between CH3I and T. weissflogii and P. tricornuntum during the experiment, and there was a 40 % reduction in mean CH3I concentration in the high pCO2 mesocosms. CHBrCl2, CH3Br, and CH2Br2 concentrations did not increase with elevated chlorophyll a (Chl a) concentrations compared with DMS(P) and CH3I, and there were no major peaks both in the high pCO2 or low pCO2 mesocosms. In addition, no effect of elevated pCO2 was identified for any of the three bromocarbons.

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Bartual, Ana, and J. Angel Gálvez. "Short- and long-term effects of irradiance and CO2 availability on carbon fixation by two marine diatoms." Canadian Journal of Botany 81, no. 3 (March 1, 2003): 191–200. http://dx.doi.org/10.1139/b03-013.

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Unbuffered and nutrient-replete dilute batch cultures of Skeletonema costatum Cleve and Phaeodactylum tricornutum Bohlin were grown at high and low CO2 availability conditions and two incident irradiances, 150 and 30 µmol photons·m2·s1. Long-term combined effects of such light and CO2 availability conditions on carbon fixation rates of both diatoms were compared. At saturating light, P. tricornutum showed higher photosynthetic rates than S. costatum at both CO2 conditions. However, under subsaturating light, carbon fixation rates of P. tricornutum were higher than observed for S. costatum only at low CO2. Skeletonema costatum showed a strong reduction in photosynthetic rates only when both resources, irradiance and CO2, were low. Short-term alterations of light and CO2 availability on carbon fixation showed that the response of S. costatum differed considerably from long-term trends: the short-term reduction in CO2 availability at both light levels resulted in a considerable decrease in the maximum photosynthetic rates. This effect was much less noticeable in P. tricornutum. The results show that, at saturating light, both diatoms maintain maximum photosynthetic rates under low CO2 levels, but only P. tricornutum is well adapted to rapid changes in this resource. This capacity of adaptation seems to be light dependent, since light limitation altered the responses of both diatoms to low CO2 availability conditions.Key words: CO2, 14C fixation, irradiance, Phaeodactylum tricornutum, Skeletonema costatum.

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Uslu, Leyla, Gökhan Tamer Kayaalp, Oya Işık, Burcu Ak Çimen, and Melis Çelik Güney. "Isochrysis affinis galbana ve Phaeodactylum tricornutum Kültürlerinde Kuru Madde Tahminlerinin Karşılaştırılması." Turkish Journal of Agriculture - Food Science and Technology 6, no. 8 (August 21, 2018): 1088. http://dx.doi.org/10.24925/turjaf.v6i8.1088-1091.2039.

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Isochrysis affinis galbana is a single-cell marine species. Isochrysis affinis galbana is used as a live feed source in aquaculture because it contains high amount of long chain unsaturated fatty acids (PUFA), especially in the feeding of larvae of bivalves, Phaeodactylum tricornutum is a diatom species of pennate with oval and fusiform cell forms. Phaeodactylum tricornutum contains 30-45% long chain unsaturated fatty acids (PUFA) of which 20-40% is eicosapentaenoic acid (EPA). And the diatom which is thought to be sources of essential fatty acids and carotenoids (fucoxanthin) is used in the feeding of animal in aquaculture and human. The aim of this study was to estimate the dry matter content of cultured Isochrysis affinis galbana and Phaeodactylum tricornutum by using multiple regression analysis method. Species were cultured in the laboratory conditions with a control group with 20% inoculation rate in F/2 medium and 50% nitrogen deficient medium. During the experiment, optical density, dry matter and chlorophyll a were measured daily. A mathematical model was developed using optic density and chlorophyll a data of the control group and in the group where 50% of the nitrogen was eliminated. Significance test of these models, R2 and MSE were found.

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Kovalev, N. N., S. E. Leskova, E. V. Mikheev, Yu M. Pozdnyakova, and R. V. Esipenko. "CULTURAL AND BIOCHEMICAL PARAMETERS OF TWO MICROALGAE, PHAEODACTYLUM TRICORNUTUM AND TETRASELMIS SUECIACA, IN CUMULATIVE CULTURES." Bulletin оf Kamchatka State Technical University, no. 53 (2020): 54–65. http://dx.doi.org/10.17217/2079-0333-2020-53-54-65.

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A comparative study of cultural and biochemical parameters of two microalgal species, Tetraselmis sueciaca and Phaeodactylum tricornutum, was performed. Cell density in the storage culture was 2.88 million cells per one ml for T. sueciaca and 20.37 million cells per one ml for Ph. tricornutum. As shown, determination of op-tical density of the culture medium is a more objective characteristic of the cumulative culture growth. For T. sueciaca, cell growth rate during the course of experiment made 400% and for Ph. tricornutum – 700%. Dif-ference in the chemical composition of microalgal biomass was revealed. The microalgae did not differ in their energy value. The prospects of cumulative culture of microalgae in mariculture farms are discussed.

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Zhao, Xinjie, Liwei Gao, and Xiangzhong Zhao. "Rapid Purification of Fucoxanthin from Phaeodactylum tricornutum." Molecules 27, no. 10 (May 17, 2022): 3189. http://dx.doi.org/10.3390/molecules27103189.

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Fucoxanthin is a natural marine xanthophyll and exhibits a broad range of biological activities. In the present study, a simple and efficient two-step method was used to purify fucoxanthin from the diatom, Phaeodactylum tricornutum. The crude pigment extract of fucoxanthin was separated by silica gel column chromatography (SGCC). Then, the fucoxanthin-rich fraction was purified using a hydrophile–lipophile balance (HLB) solid-phase extraction column. The identification and quantification of fucoxanthin were determined by high-performance liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS). This two-step method can obtain 92.03% pure fucoxanthin and a 76.67% recovery rate. In addition, 1H and 13C NMR spectrums were adopted to confirm the identity of fucoxanthin. Finally, the purified fucoxanthin exhibited strong antioxidant properties in vitro with the effective concentration for 50% of maximal scavenging (EC50) of 1,1-Dihpenyl-2-picrylhydrazyl (DPPH) and 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radicals being 0.14 mg·mL−1 and 0.05 mg·mL−1, respectively.

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Singh, Dipali, Ross Carlson, David Fell, and Mark Poolman. "Modelling metabolism of the diatom Phaeodactylum tricornutum." Biochemical Society Transactions 43, no. 6 (November 27, 2015): 1182–86. http://dx.doi.org/10.1042/bst20150152.

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Marine diatoms have potential as a biotechnological production platform, especially for lipid-derived products, including biofuels. Here we introduce some features of diatom metabolism, particularly with respect to photosynthesis, photorespiration and lipid synthesis and their differences relative to other photosynthetic eukaryotes. Since structural metabolic modelling of other photosynthetic organisms has been shown to be capable of representing their metabolic capabilities realistically, we briefly review the main approaches to this type of modelling. We then propose that genome-scale modelling of the diatom Phaeodactylum tricornutum, in response to varying light intensity, could uncover the novel aspects of the metabolic potential of this organism.

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Scala, Simona, Nicolas Carels, Angela Falciatore, Maria Luisa Chiusano, and Chris Bowler. "Genome Properties of the Diatom Phaeodactylum tricornutum." Plant Physiology 129, no. 3 (June 14, 2002): 993–1002. http://dx.doi.org/10.1104/pp.010713.

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Cerón Garcı́a, M. C., A. Sánchez Mirón, J. M. Fernández Sevilla, E. Molina Grima, and F. Garcı́a Camacho. "Mixotrophic growth of the microalga Phaeodactylum tricornutum." Process Biochemistry 40, no. 1 (January 2005): 297–305. http://dx.doi.org/10.1016/j.procbio.2004.01.016.

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Ozcan, Ova D., A. Ata, and B. Ovez. "Identification of Photosynthetic Pigments extracted from Phaeodactylum tricornutum as High-Value Bioactive Compounds." Research Journal of Biotechnology 17, no. 12 (November 25, 2022): 91–99. http://dx.doi.org/10.25303/1712rjbt91099.

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The present study intends to provide information about a promising source of unique bioactive compounds and for choosing the favorable growth conditions of the diatom “Phaeodactylum tricornutum” suited to different applications. P. tricornutum was cultivated in the temperature range of 20-35°C, light intensity range of 275-538 μmolm-2s-1 and aeration rate range of 2.5- 3.5 Lmin-1. P. tricornutum extracts were analyzed in terms of photosynthetic pigment composition in comparison with a well-known green microalgae Chlorella miniata using the C18 column. Lutein, canthaxanthin, β-carotene and chlorophyll a were detected in C. miniata whereas in P. tricornutum extracts, chlorophyll a, zeaxanthin and β-carotene were identified. Quantitatively, P. tricornutum was distinguished to have greater amount of chlorophyll-a (113.66 mgmL-1) while C. miniata was predominated with a β-carotene content (14.20 mgmL-1). Considerable amounts of pigments were encountered in all of the P. tricornutum extracts obtained under the varying growth conditions. As the temperature was increased and the light intensity was decreased, the accumulation of chlorophyll a and β-carotene increased at the highest aeration rate. Conversely, at the highest light intensity of 538 μmolm-2s-1 and an aeration rate of 3.0 to 3.5 Lmin-1, the zeaxanthin content was maximized and limited at the temperature values over 27.5°C.

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Fitria, Murni, Anggi Saputra, Rahmat Hidayat, and Niken Feladita. "Analisis Protein Gen LCYB pada Mikroalga Laut Phaeodactylum tricornutum menggunakan Pendekatan Bioinformatika." IJCA (Indonesian Journal of Chemical Analysis) 5, no. 2 (September 23, 2022): 138–44. http://dx.doi.org/10.20885/ijca.vol5.iss2.art8.

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LCYB merupakan gen yang memproduksi enzim Lycopene Beta Cyclase. Enzim Lycopene Beta Cyclase beperan dalam mengubah likopen menjadi ß-karoten. Gen LCYB terdapat pada diatom maupun mikroalga hijau. Berbeda dengan mikroalga hijau, penelitian mengenai struktur protein Lycopene Beta Cyclase selaku produk gen LCYB pada diatom masih minim publikasi. Dalam penelitian ini dilakukan prediksi struktur produk gen LCYB pada diatom P. tricornutum secara bioinformatika. Untuk menganalisis perbedaan produk gen LCYB pada diatom dan mikroalga hijau, produk gen LCYB pada diatom P. tricornutum dibandingkan dengan produk gen LCYB pada mikroalga hijau M. zofingiensis. Konstruksi pohon filogenetik dilakukan menggunakan perangkat lunak MEGA7. Analisis gen LCYB dan struktur produk gen LCYB dilakukan menggunakan beberapa perangkat bioinformatika. Analisis docking dilakukan untuk memperkuat prediksi adanya interaksi antara produk gen LCYB hasil prediksi dengan ligannya menggunakan AutoDocksTools-1.5.6. M. zofingiensis memiliki hubungan kekerabatan yang jauh dengan P. Tricornutum sehingga kemiripan gen dan produk gen LCYB hasil prediksi juga jauh berbeda. Mikroalga hijau M. zofingiensis mengandung protein transmembran yang tidak dimiliki pada diatom P. Tricornutum. Hasil docking menunjukkan adanya interaksi antara produk gen LCYB hasil prediksi dengan ligannya yaitu likopen.

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Huang, Aiyou, Yujue Wang, Jiawen Duan, Shiyi Guo, and Zhenyu Xie. "Differential Response of Phaeodactylum tricornutum and Cylindrotheca fusiformis to High Concentrations of Cu2+ and Zn2+." Water 14, no. 20 (October 19, 2022): 3305. http://dx.doi.org/10.3390/w14203305.

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Diatoms can be used as biosensors to assess aquatic environment quality, because they are widely distributed in almost all aquatic environments and show varied sensitivities toward heavy metal ions. The marine planktonic diatoms Phaeodactylum tricornutum (P. tricornutum) and Cylindrotheca fusiformis (C. fusiformis) are typical representatives of planktonic diatoms and benthic diatoms, respectively. C. fusiformis is very sensitive to changes in the concentration of heavy metal ions, and can be used as an indicator of the quality of the sedimental environment, while P. tricornutum can tolerate higher concentrations of heavy metal ions. To explore the potential difference in responses to heavy metal ions between planktonic and benthic diatoms, we compared the transcriptome of P. tricornutum and C. fusiformis under Cu2+ and Zn2+ treatment. The results indicated that P. tricornutum has several genes involved in ion transmembrane transport and ion homeostasis, which are significantly downregulated under Cu2+ and Zn2+ treatment. However, this enrichment of ion transmembrane transport- and ion homeostasis-related genes was not observed in C. fusiformis under Cu2+ and Zn2+ treatment. Additionally, genes related to heavy metal ion stress response such as peroxiredoxin, peroxidase, catalase, glutathione metabolism, phytochelatin, oxidative stress and disulfide reductase, were upregulated in P. tricornutum under Cu2+ and Zn2+ treatment, whereas most of them were downregulated in C. fusiformis under Cu2+ and Zn2+ treatment. This difference in gene expression may be responsible for the difference in sensitivity to heavy metals between P. tricornutum and C. fusiformis.

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Giguere, Daniel J., Alexander T. Bahcheli, Samuel S. Slattery, Rushali R. Patel, Tyler S. Browne, Martin Flatley, Bogumil J. Karas, David R. Edgell, and Gregory B. Gloor. "Telomere-to-telomere genome assembly of Phaeodactylum tricornutum." PeerJ 10 (July 5, 2022): e13607. http://dx.doi.org/10.7717/peerj.13607.

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Phaeodactylum tricornutum is a marine diatom with a growing genetic toolbox available and is being used in many synthetic biology applications. While most of the genome has been assembled, the currently available genome assembly is not a completed telomere-to-telomere assembly. Here, we used Oxford Nanopore long reads to build a telomere-to-telomere genome for Phaeodactylum tricornutum. We developed a graph-based approach to extract all unique telomeres, and used this information to manually correct assembly errors. In total, we found 25 nuclear chromosomes that comprise all previously assembled fragments, in addition to the chloroplast and mitochondrial genomes. We found that chromosome 19 has filtered long-read coverage and a quality estimate that suggests significantly less haplotype sequence variation than the other chromosomes. This work improves upon the previous genome assembly and provides new opportunities for genetic engineering of this species, including creating designer synthetic chromosomes.

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Harada, Hisashi, and Yusuke Matsuda. "Identification and characterization of a new carbonic anhydrase in the marine diatom Phaeodactylum tricornutum." Canadian Journal of Botany 83, no. 7 (July 1, 2005): 909–16. http://dx.doi.org/10.1139/b05-078.

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A cDNA encoding a new isoenzyme of β-type carbonic anhydrase (CA; EC 4.2.1.1) in the marine diatom Phaeodactylum tricornutum Bohlin has been cloned. The cDNA contained an open reading frame of 819 bp, which encodes a polypeptide of 273 amino acids. This gene, which is designated as ptca2, was found to be highly homologous (83% at the nucleotide level) to the previously isolated intracellular β-CA gene from Phaeodactylum tricornutum (ptca1). Comparison of the deduced amino acid sequence of ptca2 with β-CAs from other sources demonstrated that PtCA2 possesses the completely conserved zinc coordination residues of β-CA. The N-terminus 19 amino acid sequence of PtCA2 was predicted to be an endoplasmic reticulum-targeting signal, suggesting localization of the protein in an organelle or in the periplasmic space. Quantitative analysis of mRNA accumulation of ptca2 using real-time polymerase chain reaction revealed a significant level of mRNA accumulation even under 5% CO2 and a 3.5-fold increase in accumulation upon acclimation of the diatom to air. This indicates that ptca2 belongs to a constitutive class of enzyme that responds only weakly to the ambient CO2 concentration. The sequences of both ptca1 and ptca2 were shown to be grouped into a phylogeny that is composed of mixture of sequences from the eucarya and procarya domains, including sequences from the red alga Porphyridium purpureum, the green alga Coccomyxa, the red mold Neurospora crassa, and the yeast Saccharomyces cerevisiae.Key words: carbonic anhydrase, marine diatom, inorganic carbon concentrating mechanism (CCM), Phaeodactylum tricornutum.

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Coesel, Sacha N. "More than a photoreceptor: aureochromes are intrinsic to the diatom light-regulated transcriptional network." Journal of Experimental Botany 75, no. 7 (March 27, 2024): 1786–90. http://dx.doi.org/10.1093/jxb/erae004.

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This article comments on: Im SH, Lepetit B, Mosesso N, Shrestha S, Weiss L, Nymark M, Roellig R, Wilhelm C, Isono E, Kroth PG. 2024. Identification of promoter targets by Aureochrome 1a in the diatom Phaeodactylum tricornutum. Journal of Experimental Botany 75, 1834–1851.

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Felizardo, Jéssica Reis, Vinícius José Coelho, Ângelo P. Matos, and Elisa Helena Siegel Moecke. "INFLUÊNCIA DA SALINIDADE NA PRODUÇÃO DE BIOMASSA E DE LIPÍDIOS DURANTE O CULTIVO DAS MICROALGAS Tetraselmis gracilis E Phaeodactylum tricornutum." Revista Gestão & Sustentabilidade Ambiental 9, no. 0I (August 18, 2020): 140. http://dx.doi.org/10.19177/rgsa.v9e0i2020140-155.

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As microalgas são organismos fotossintetizantes que apresentam diversas aplicações econômicas, como utilização na alimentação humana, animal e na produção de biocombustíveis devido a sua biomassa rica em subprodutos. O presente estudo teve como objetivo avaliar a produtividade das espécies Tetraselmis gracilis e Phaeodactylum tricornutum frente as salinidades de 17o/oo, 26 o/oo e 35o/oo, aplicadas nos meios de cultivo. Os cultivos foram realizados em meio F/2 Guillard modificado utilizando água do mar filtrada com salinidade natural de 17o/oo. A densidade celular dos cultivos foi monitorada através da contagem de células, a biomassa e o teor de lipídios foram obtidos na fase estacionária dos cultivos. Os cultivos com maior produtividade de biomassa foram os que apresentaram menor teor de lipídios da biomassa seca. Desta forma, visando a produção de lipídios a salinidade ideal para espécie Tetraselmis gracilis é de 17o/oo com teor de lipídios de 27,88% e para a espécie Phaeodactylum tricornutum seria aplicável 26o/oo pois apresentou 12,26%.

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Mercado, Borja, Nuria Valero, Luis Roca-Pérez, Elena Bernabeu-Berni, and Oscar Andreu-Sánchez. "Investigation of Metal Toxicity on Microalgae Phaeodactylum tricornutum, Hipersaline Zooplankter Artemia salina, and Jellyfish Aurelia aurita." Toxics 11, no. 8 (August 20, 2023): 716. http://dx.doi.org/10.3390/toxics11080716.

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The escalating global anthropogenic activities associated with industrial development have led to the increased introduction of heavy metals (HMs) into marine environments through effluents. This study aimed to assess the toxicity of three HMs (Cr, Cu, and Cd) on organisms spanning different trophic levels: Phaeodactylum tricornutum (a primary producer), Artemia salina (a primary consumer), and Aurelia aurita (a secondary consumer). The EC50 values obtained revealed varying relative toxicities for the tested organisms. Phaeodactylum tricornutum exhibited the highest sensitivity to Cu, followed by Cd and Cr, while Artemia salina displayed the highest sensitivity to Cr, followed by Cu and Cd. A. aurita, on the other hand, demonstrated the highest sensitivity to Cu, followed by Cr and Cd. This experimental investigation further supported previous studies that have suggested A. aurita as a suitable model organism for ecotoxicity testing. Our experiments encompassed sublethal endpoints, such as pulsation frequency, acute effects, and mortality, highlighting different levels of sensitivity among the organisms.

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Buck, Jochen M., Carolina Río Bártulos, Ansgar Gruber, and Peter G. Kroth. "Blasticidin-S deaminase, a new selection marker for genetic transformation of the diatom Phaeodactylum tricornutum." PeerJ 6 (November 14, 2018): e5884. http://dx.doi.org/10.7717/peerj.5884.

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Most genetic transformation protocols for the model diatom Phaeodactylum tricornutum rely on one of two available antibiotics as selection markers: Zeocin (a formulation of phleomycin D1) or nourseothricin. This limits the number of possible consecutive genetic transformations that can be performed. In order to expand the biotechnological possibilities for P. tricornutum, we searched for additional antibiotics and corresponding resistance genes that might be suitable for use with this diatom. Among the three different antibiotics tested in this study, blasticidin-S and tunicamycin turned out to be lethal to wild-type cells at low concentrations, while voriconazole had no detectable effect on P. tricornutum. Testing the respective resistance genes, we found that the blasticidin-S deaminase gene (bsr) effectively conferred resistance against blasticidin-S to P. tricornutum. Furthermore, we could show that expression of bsr did not lead to cross-resistances against Zeocin or nourseothricin, and that genetically transformed cell lines with resistance against Zeocin or nourseothricin were not resistant against blasticidin-S. In a proof of concept, we also successfully generated double resistant (against blasticidin-S and nourseothricin) P. tricornutum cell lines by co-delivering the bsr vector with a vector conferring nourseothricin resistance to wild-type cells.

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Kovalev, Nikolai Nikolayevich, Svetlana Evgenyevna Leskova, and Evgeny Valerevich Mikheev. "Effect of auxin plant hormones on growth and biochemical composition of microalgae Phaeodactylum tricornutum Bohlin, 1897." Vestnik of Astrakhan State Technical University. Series: Fishing industry 2023, no. 3 (October 13, 2023): 97–105. http://dx.doi.org/10.24143/2073-5529-2023-3-97-105.

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Regulation of the growth rate of Phaeodactylum tricornutum opens up prospects for the organization of efficient industrial production of live feed biomass. We studied the effect of two plant hormones, indole-3-butyric acid (3-IBA) and indole-3-acetic acid (3-IAA), on the growth and biochemical composition of Ph. tricornutum in an enrichment culture. The effective 3-IBA concentration of 0.2 ∙ 10–5 M increased the density of the culture by 373% on day 14of cultivation compared to the control. High concentrations of 3-IAA either inhibited the development of the culture or did not have a significant effect on its growth. A stimulating effect of exposure to 3-IAA at 0.1 ∙ 10–5 M on the growth was recorded on day 12 of cultivation and amounted to 328.2% compared to the control. A stimulating effect of 3-IBA exposure on protein accumulation was recorded on day 4 of cultivation and amounted to 27.9% compared to the control. A stimulating effect of 3-IAA on protein synthesis was recorded on day 12 of cultivation of Ph. tricornutum. The protein concentration during this period was 1.2-fold higher than in the control. Indole-3-butyric acid did not have any effect on lipid accumulation, compared to the control, throughout the cultivation time. On day 14 of cultivation under the exposure to 3-IAA, the concentration of lipids was 1.5-fold higher than in the control and in the group exposed to 3-IBA. Indole-3-butyric acid did not show any effect on the accumulation of carbohydrates in the Ph. tricornutum culture throughout the cultivation period. On day 4 of cultivation, the carbohydrate concentration in the culture exposed to 3-IAA increased by 240% compared to the control. At the end of the experiment (14 days), no significant differences between the experi-mental cultures in the carbohydrate concentration were observed. For 8 days of Ph. tricornutum cultivation, the chlorophyll concentration increased by 927.4 and 1 178.6% as a result of exposure to 3-IBA and 3-IAA, respec-tively.

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De Riso, Valentina, Raffaella Raniello, Florian Maumus, Alessandra Rogato, Chris Bowler, and Angela Falciatore. "Gene silencing in the marine diatom Phaeodactylum tricornutum." Nucleic Acids Research 37, no. 14 (May 31, 2009): e96-e96. http://dx.doi.org/10.1093/nar/gkp448.

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Owens, Thomas G., and Evarina R. Wold. "Light-Harvesting Function in the Diatom Phaeodactylum tricornutum." Plant Physiology 80, no. 3 (March 1, 1986): 732–38. http://dx.doi.org/10.1104/pp.80.3.732.

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Owens, Thomas G. "Light-Harvesting Function in the Diatom Phaeodactylum tricornutum." Plant Physiology 80, no. 3 (March 1, 1986): 739–46. http://dx.doi.org/10.1104/pp.80.3.739.

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Tesson, Benoit, C. Gaillard, and V. Martin-Jézéquel. "Brucite formation mediated by the diatom Phaeodactylum tricornutum." Marine Chemistry 109, no. 1-2 (February 2008): 60–76. http://dx.doi.org/10.1016/j.marchem.2007.12.005.

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Branco-Vieira, Monique, Sergio San Martin, Cristian Agurto, Marco A. Santos, Marcos A. V. Freitas, and Nídia S. Caetano. "Analyzing Phaeodactylum tricornutum lipid profile for biodiesel production." Energy Procedia 136 (October 2017): 369–73. http://dx.doi.org/10.1016/j.egypro.2017.10.251.

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Branco-Vieira, Monique, Sergio San Martin, Cristian Agurto, Marcos A. V. Freitas, Teresa M. Mata, António A. Martins, and Nídia Caetano. "Phaeodactylum tricornutum derived biosilica purification for energy applications." Energy Procedia 153 (October 2018): 279–83. http://dx.doi.org/10.1016/j.egypro.2018.10.020.

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Montsant, Anton, Kamel Jabbari, Uma Maheswari, and Chris Bowler. "Comparative Genomics of the Pennate Diatom Phaeodactylum tricornutum." Plant Physiology 137, no. 2 (January 21, 2005): 500–513. http://dx.doi.org/10.1104/pp.104.052829.

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REBOLLOSO-FUENTES, M. M., A. NAVARRO-PÉREZ, J. J. RAMOS-MIRAS, and J. L. GUIL-GUERRERO. "BIOMASS NUTRIENT PROFILES OF THE MICROALGA PHAEODACTYLUM TRICORNUTUM." Journal of Food Biochemistry 25, no. 1 (February 2001): 57–76. http://dx.doi.org/10.1111/j.1745-4514.2001.tb00724.x.

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Francius, Grégory, Benoît Tesson, Etienne Dague, Véronique Martin-Jézéquel, and Yves F. Dufrêne. "Nanostructure and nanomechanics of live Phaeodactylum tricornutum morphotypes." Environmental Microbiology 10, no. 5 (May 2008): 1344–56. http://dx.doi.org/10.1111/j.1462-2920.2007.01551.x.

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Branco-Vieira, Monique, Sergio San Martin, Cristian Agurto, Marcos A. V. Freitas, António A. Martins, Teresa M. Mata, and Nídia S. Caetano. "Biotechnological potential of Phaeodactylum tricornutum for biorefinery processes." Fuel 268 (May 2020): 117357. http://dx.doi.org/10.1016/j.fuel.2020.117357.

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Si, DongFang, LiMin Yang, Hua Yan, and QiuQuan Wang. "Bioaccumulation and transformation of cadmium by Phaeodactylum tricornutum." Science in China Series B: Chemistry 52, no. 12 (December 2009): 2373–80. http://dx.doi.org/10.1007/s11426-010-0015-1.

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Oroszi, Steffen, Torsten Jakob, Christian Wilhelm, Hauke Harms, and Thomas Maskow. "Photosynthetic energy conversion in the diatom Phaeodactylum tricornutum." Journal of Thermal Analysis and Calorimetry 104, no. 1 (February 15, 2011): 223–31. http://dx.doi.org/10.1007/s10973-011-1358-3.

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Libralato, Giovanni, Francesco Avezzù, and Annamaria Volpi Ghirardini. "Lignin and tannin toxicity to Phaeodactylum tricornutum (Bohlin)." Journal of Hazardous Materials 194 (October 2011): 435–39. http://dx.doi.org/10.1016/j.jhazmat.2011.07.103.

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Liu, Hao, Yawen Chen, Heyu Wang, Yaxuan Huang, Ying Hu, Yuxiang Zhao, and Yifu Gong. "Identification of Potential Factors for the Promotion of Fucoxanthin Synthesis by Methyl Jasmonic Acid Treatment of Phaeodactylum tricornutum." Marine Drugs 22, no. 1 (December 20, 2023): 7. http://dx.doi.org/10.3390/md22010007.

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Fucoxanthin, a vital secondary metabolite produced by marine diatoms, has great economic value and research potential. However, its popularization and application have been greatly restricted due to its low content, difficult extraction, and high production cost. Methyl jasmonic acid (MeJA) exerts similar inductive hormones in the growth and development as well as metabolic processes of plants. In Phaeodactylum tricornutum (P. tricornutum), MeJA treatment can increase fucoxanthin content. In this study, the effects of different concentrations of MeJA on the cell growth and the fucoxanthin content of P. tricornutum were explored. Meanwhile, this study used high-throughput sequencing technology for transcriptome sequencing of P. tricornutum and subsequently performed differential gene expression analysis, gene ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, and weighted gene co-expression network analysis (WGCNA) for screening the hub genes for the promotion of fucoxanthin synthesis with MeJA-treated P. tricornutum. On this basis, the functions of the hub genes for the promotion of fucoxanthin synthesis with MeJA-treated P. tricornutum were further analyzed. The results revealed that the carotenoid synthesis-related genes PHATRDRAFT_54800 and PHATRDRAFT_20677 were the hub genes for the promotion of fucoxanthin synthesis with MeJA-treated P. tricornutum. PHATRDRAFT_54800 may be a carotenoid isomerase, while PHATRDRAFT_20677 may be involved in the MeJA-stimulated synthesis of fucoxanthin by exerting the role of SDR family NAD(P)-dependent oxidoreductases.

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Curcuraci, Eleonora, Simona Manuguerra, Concetta Maria Messina, Rosaria Arena, Giuseppe Renda, Theodora Ioannou, Vito Amato, Claire Hellio, Francisco J. Barba, and Andrea Santulli. "Culture Conditions Affect Antioxidant Production, Metabolism and Related Biomarkers of the Microalgae Phaeodactylum tricornutum." Antioxidants 11, no. 2 (February 17, 2022): 411. http://dx.doi.org/10.3390/antiox11020411.

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Phaeodactylum tricornutum (Bacillariophyta) is a worldwide-distributed diatom with the ability to adapt and survive in different environmental habitats and nutrient-limited conditions. In this research, we investigated the growth performance, the total lipids productivity, the major categories of fatty acids, and the antioxidant content in P. tricornutum subjected for 15 days to nitrogen deprivation (N−) compared to standard culture conditions (N+). Furthermore, genes and pathways related to lipid biosynthesis (i.e., glucose-6-phosphate dehydrogenase, acetyl-coenzyme A carboxylase, citrate synthase, and isocitrate dehydrogenase) and photosynthetic activity (i.e., ribulose-1,5-bisphospate carboxylase/oxygenase and fucoxanthin-chlorophyll a/c binding protein B) were investigated through molecular approaches. P. tricornutum grown under starvation condition (N−) increased lipids production (42.5 ± 0.19 g/100 g) and decreased secondary metabolites productivity (phenolic content: 3.071 ± 0.17 mg GAE g−1; carotenoids: 0.35 ± 0.01 mg g−1) when compared to standard culture conditions (N+). Moreover, N deprivation led to an increase in the expression of genes involved in fatty acid biosynthesis and a decrease in genes related to photosynthesis. These results could be used as indicators of nitrogen limitation for environmental or industrial monitoring of P. tricornutum.

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German-Báez, LJ, MA Valdez-Flores, JV Félix-Medina, CD Norzagaray-Valenzuela, DU Santos-Ballardo, C. Reyes-Moreno, LM Shelton, and A. Valdez-Ortiz. "Chemical composition and physicochemical properties of Phaeodactylum tricornutum microalgal residual biomass." Food Science and Technology International 23, no. 8 (June 28, 2017): 681–89. http://dx.doi.org/10.1177/1082013217717611.

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The production of photosynthetic biofuels using microalgae is a promising strategy to combat the use of non-renewable energy sources. The microalgae residual biomass is a waste by-product of biofuel production; however, it could prove to have utility in the development of sustainable nutraceuticals and functional foods. In this study, a comprehensive characterisation of the under-utilised Phaeodactylum tricornutum microalgae residual biomass is presented. Proximal composition, antioxidant capacity (using three different antioxidant assays; oxygen radical absorbance capacity; radical cation activity, ABTS; and radical scavenging activity, DPPH), and total phenolic content of free and bound polyphenols were determined. Additionally, the physicochemical properties of water activity, pH, water absorption index, water solubility index, and dispersibility were evaluated. Results revealed that P. tricornutum microalgae residual biomass exhibits a relatively high protein and carbohydrate content, with values of 36.67% and 46.78%, respectively; and most carbohydrates were found as total dietary fibre (45.57%), of which insoluble dietary fibre was the most predominant (43.54%). Antioxidant capacity values for total phytochemicals of 106.22, 67.93, 9.54 µM TE g−1 dw were determined by oxygen radical absorbance capacity, ABTS, and DPPH assays, respectively. Total phenolic content was found to be 2.90 mg GAE g−1 dw. Interestingly, antioxidant capacity and total phenolic content were higher in bound than in free phytochemical extracts. The physicochemical analysis showed P. tricornutum microalgae residual biomass to have suitable properties for the generation of a beverage with Aw, pH, water absorption index, water solubility index, and dispersibility values of 0.45, 7.12, 3.40 g gel g−1 dw, 2.5 g solids 100 g−1 dw, and 90%, respectively. Hence, P. tricornutum microalgae residual biomass could be considered a potential source of bioactive compounds suitable for the production of functional food exhibiting antioxidant capacity and high dietary fibre content.

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Sittmann, John, Munhyung Bae, Emily Mevers, Muzi Li, Andrew Quinn, Ganesh Sriram, Jon Clardy, and Zhongchi Liu. "Bacterial diketopiperazines stimulate diatom growth and lipid accumulation." Plant Physiology 186, no. 2 (February 23, 2021): 1159–70. http://dx.doi.org/10.1093/plphys/kiab080.

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Abstract Diatoms are photosynthetic microalgae that fix a significant fraction of the world’s carbon. Because of their photosynthetic efficiency and high-lipid content, diatoms are priority candidates for biofuel production. Here, we report that sporulating Bacillus thuringiensis and other members of the Bacillus cereus group, when in co-culture with the marine diatom Phaeodactylum tricornutum, significantly increase diatom cell count. Bioassay-guided purification of the mother cell lysate of B. thuringiensis led to the identification of two diketopiperazines (DKPs) that stimulate both P. tricornutum growth and increase its lipid content. These findings may be exploited to enhance P. tricornutum growth and microalgae-based biofuel production. As increasing numbers of DKPs are isolated from marine microbes, the work gives potential clues to bacterial-produced growth factors for marine microalgae.

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Borodina, A. V. "The cultivating conditions effect on accumulating the carotenoids in Phaeodactylum tricornutum Bohl. (Bacillariophyta)." Algologia 23, no. 2 (June 24, 2013): 138–49. http://dx.doi.org/10.15407/alg23.02.138.

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Cabrera, Joaquin, Susana Puntarulo, and Paula Mariela González. "Domoic Acid Oxidative Effects on the Microalgae Phaeodactylum tricornutum." Life 13, no. 3 (March 2, 2023): 676. http://dx.doi.org/10.3390/life13030676.

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Domoic acid (DA) is a natural occurring marine biotoxin. Oxidative stress generation due to DA exposure was reported in animals, but little is known on the phytoplankton community. The aim of this work was to verify whether exposure to DA in the marine diatom Phaeodactylum tricornutum favors reactive oxygen species (ROS) generation in the intracellular environment modifying its antioxidant capacity. Active species production, non-enzymatic antioxidant content, and antioxidant enzyme activities over the three growth phases of P. tricornutum exposed to 64 µM DA were evaluated. Results obtained in exponential growing cells showed a time-depending seven-fold increase in the 2′,7′ dichlorofluorescein diacetate dye oxidation rate. Superoxide dismutase and catalase activities showed a two-fold increase, and glutathione related enzymes activities were also significantly increased in treated diatoms as compared to controls. However, glutathione and ascorbate contents significantly decreased after incubation of the cells with DA. Similar effects were observed in latent and stationary phases of cell development. These results showed that DA could cause a severe oxidant-dependent impact on a non-toxic algae.

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Sharma, Nikunj, Gabriel Fleurent, Fatima Awwad, Michael Cheng, Fatma Meddeb-Mouelhi, Suzanne M. Budge, Hugo Germain, and Isabel Desgagné-Penix. "Red Light Variation an Effective Alternative to Regulate Biomass and Lipid Profiles in Phaeodactylum tricornutum." Applied Sciences 10, no. 7 (April 7, 2020): 2531. http://dx.doi.org/10.3390/app10072531.

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Marine water diatom Phaeodactylum tricornutum is a photosynthetic organism that is known to respond to the changing light environment and adapt to different temperatures to prevent photoinhibition and maintain its metabolic functions. The objective of the present study was to test whether light shift variations in different growth phases impact the growth and lipid metabolism of P. tricornutum. Thus, we investigated R exposure in different growth phases to find the most effective light shift condition. The results showed that substituting white light (W) by red light (R) under autotrophic conditions, a condition called red shift (RS), increased biomass and lipid content compared to levels found under continuous W or R exposure alone. We observed an increase by 2-fold biomass and 2.3-fold lipid content in RS as compared to W. No significant change was observed in the morphology of lipid droplets, but the fatty acid (FA) composition was altered. Specifically, polyunsaturated FAs were increased, whereas monounsaturated FAs decreased in P. tricornutum grown in RS compared to W control. Therefore, we propose that a light shift during the beginning of the stationary phase is a low-cost cultivation strategy to boost the total biomass and lipids in P. tricornutum.

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Li, Na, Shimeng Chen, Jun Yang, Jun Song, and Yongxin Song. "Impacts of Chlorine on the Change of Chlorophyll Fluorescence Spectrum to Phaeodactylum tricornutum." Analytica 4, no. 2 (April 11, 2023): 102–12. http://dx.doi.org/10.3390/analytica4020009.

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Chlorine-containing disinfectants have been widely used all over the world to prevent COVID-19. However, little is known about the potential risk of chlorine-containing disinfectants in the marine environment. Phaeodactylum tricornutum (P. tricornutum) is a typical marine economic diatom, often used as an effective biomarker in ecotoxicology research. Here, the present study has investigated the effect of different effective chlorine concentrations on photosynthesis of P. tricornutum by chlorophyll fluorescence spectroscopy. Results have demonstrated that chlorine exposure promoted the chlorophyll fluorescence intensity at initial stage (24 h), suggesting that a large amount of energy is emitted in the form of fluorescence. However, the chlorophyll fluorescence intensity could not be detected under the high effective chlorine concentrations (6.7 × 10−3, 1.0 × 10−2, 1.3 × 10−2 and 1.7 × 10−2 mg L−1) after 48 h, indicating that the chlorine had high toxicity leading to the death of microalgae. In addition, the emission spectra of P. tricornutum were determined to contain two distinct fluorescence peaks representing the core antenna of photosystem II (685 nm) and the photosystem I complexes (710 nm) in the control group. The fluorescence emission peak value at 685 nm is significantly lower than the peak value at 710 nm in the control group, whereas chlorine treatments were opposite. It can be concluded that microalgae can regulate the distribution of excitation energy between the two photosystems to ensure that algae can utilize light energy. The result also found that the peak position of fluorescence emission spectra has a blue shift in all of NaClO treatments. The fluorescence intensity of microalgae excited at 467 nm was lower than that at 439 nm in chlorine treatments, illustrating chlorophyll b antenna was more easily damaged than chlorophyll a antenna. Our findings are providing new insights into the changing mechanism of chlorophyll fluorescence on P. tricornutum under chlorine stress and valuable data for risk assessment of marine environments.

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Sigaud-Kutner, Teresa Cristina S., and Elizabeth Aidar. "The influence of osmotic shocks on the growth rate and chlorophyll-a content of planktonic algae species." Boletim do Instituto Oceanográfico 43, no. 1 (1995): 89–98. http://dx.doi.org/10.1590/s0373-55241995000100007.

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The effect of salinity changes on the growth responses and chlorophyll-α content of four species of planktonic algae (Phaeodactylum tricornutum, Tetraselmisgracilis, Minutocelluspotymorphus, Chaetoceros sp) was evaluated using a batch culture technique. P. tricornutum, showing high values of maximum growth rate (div d-1) over the entire salinity range, revealed a great capability of metabolic adjustment when subjected to osmotic shocks. T. gracilis, M. polymorphus and Chaetoceros sp were able to displace the salinity optimum related to the maximum growth rate, depending on the preconditioning salinity. The four studied species showed oscillations of specific growth rate (div d-1) and chorophyll-α content (pg cell-1) related to light-dark cycles.

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Trigub, A. G., and V. I. Ipatova. "INFLUENCE OF NANOCOMPOSITE Ag/AgCl ON THE CULTURE OF MICROALGAE SCENEDESMUS QUADRICAUDA AND PHAEODACTYLUM TRICORNUTUM." Toxicological Review, no. 2 (April 28, 2018): 44–50. http://dx.doi.org/10.36946/0869-7922-2018-2-44-50.

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The toxicity of the Ag/AgCl nanocomposite was evaluated at different concentrations in chronic experiments for 41 days using standard freshwater and marine plant test organisms of Scenedesmus quadricauda (0.05, 0.1, 0.5 and 1.0 mg/l) and Phaeodactylum tricornutum (0.25, 0.5, 1.0, and 2.0 mg/L). Comparative sensitivity of test organisms in acute experiments (72 hours) in terms of LC50 was carried out. It was established that the green alga of S. quadricauda is more sensitive to the Ag/AgCl nanocomposite (LC50 = 0.02 mg/l) than the marine diatomea P. tricornutum (LC50 = 0.3 mg/l). The greatest algicidal effect on the growth of S. quadricauda culture was provided by the nanocomposite in concentrations of 1 and 0.5 mg/l, at which the culture did not grow during the experiment. And at concentrations of 0.1 and 0.05 mg/l the algostatic effect was observed for 10 and 1 days, respectively, after which the culture resumed growth. In the culture of P. tricornutum at concentrations of 1.0 and 2.0 mg/l there was a prolonged inhibition of growth, but after 25 days at 1.0 mg/l the number of cells began to increase. In the presence of 0.5 mg/l the culture resumed growth after 4 days of lag phase and overtook the number of control. At the concentration of 0.25 mg/l the growth of P. tricornutum was either at or above the control level. The difference in the response of the two species of algae can be explained both by the individual feature of the species and by the more complex composition of the marine nutrient medium, which reduces the toxicity of the nanocomposite. According to analytical electron microscopy silver from Ag/AgCl nanocomposites within a day falls inside the cells of S. quadricauda and P. tricornutum algae, passing unimpeded both through the cell wall and the cell membrane.

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Journal articles on the topic 'Phaeodactylum tricornutum'

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