Academic literature on the topic 'PHB volumetric productivity'

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Journal articles on the topic "PHB volumetric productivity"

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Nikel, Pablo I., Alejandra de Almeida, Evelia C. Melillo, Miguel A. Galvagno, and M. Julia Pettinari. "New Recombinant Escherichia coli Strain Tailored for the Production of Poly(3-Hydroxybutyrate) from Agroindustrial By-Products." Applied and Environmental Microbiology 72, no. 6 (2006): 3949–54. http://dx.doi.org/10.1128/aem.00044-06.

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ABSTRACT A recombinant E. coli strain (K24K) was constructed and evaluated for poly(3-hydroxybutyrate) (PHB) production from whey and corn steep liquor as main carbon and nitrogen sources. This strain bears the pha biosynthetic genes from Azotobacter sp. strain FA8 expressed from a T5 promoter under the control of the lactose operator. K24K does not produce the lactose repressor, ensuring constitutive expression of genes involved in lactose transport and utilization. PHB was efficiently produced by the recombinant strain grown aerobically in fed-batch cultures in a laboratory scale bioreactor
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Wang, J., and H. Q. Yu. "Cultivation of polyhydroxybutyrate-rich aerobic granular sludge in a sequencing batch reactor." Water Supply 6, no. 6 (2006): 81–87. http://dx.doi.org/10.2166/ws.2006.966.

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In this study a two-step strategy was adopted to cultivate Polyhydroxybutyrate (PHB)-rich aerobic granular sludge in a sequencing batch reactor (SBR) fed with a synthetic wastewater. In the first step both oxygen and ammonia were initially limited, in order to enhance the PHB-storage ability of sludge. In the second step granular sludge was cultivated to get a high PHB volumetric productivity. The PHB content of sludge increased to 43.1±2.0% in the first step. During the sludge granulation, the PHB content was constant at 40±4.6%. With the granulation, the settling ability of the PHB-rich slud
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Hermann-Krauss, Carmen, Martin Koller, Alexander Muhr, Hubert Fasl, Franz Stelzer, and Gerhart Braunegg. "Archaeal Production of Polyhydroxyalkanoate (PHA) Co- and Terpolyesters from Biodiesel Industry-Derived By-Products." Archaea 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/129268.

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The archaeonHaloferax mediterraneiwas selected for production of PHA co- and terpolyesters using inexpensive crude glycerol phase (CGP) from biodiesel production as carbon source. CGP was assessed by comparison with the application of pure glycerol. Applying pure glycerol, a copolyester with a molar fraction of 3-hydroxybutyrate (3HB) of 0.90 mol/mol and 3-hydroxyvalerate (3HV) of 0.10 mol/mol, was produced at a volumetric productivity of 0.12 g/Lh and an intracellular PHA content of 75.4 wt.-% in the sum of biomass protein plus PHA. Application of CGP resulted in the same polyester compositio
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Blunt, Warren, David Levin, and Nazim Cicek. "Bioreactor Operating Strategies for Improved Polyhydroxyalkanoate (PHA) Productivity." Polymers 10, no. 11 (2018): 1197. http://dx.doi.org/10.3390/polym10111197.

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Microbial polyhydroxyalkanoates (PHAs) are promising biodegradable polymers that may alleviate some of the environmental burden of petroleum-derived polymers. The requirements for carbon substrates and energy for bioreactor operations are major factors contributing to the high production costs and environmental impact of PHAs. Improving the process productivity is an important aspect of cost reduction, which has been attempted using a variety of fed-batch, continuous, and semi-continuous bioreactor systems, with variable results. The purpose of this review is to summarize the bioreactor operat
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Miranda, Diego A., Katherine Marín, Ola Sundman, et al. "Production and Characterization of Poly(3-hydroxybutyrate) from Halomonas boliviensis LC1 Cultivated in Hydrolysates of Quinoa Stalks." Fermentation 9, no. 6 (2023): 556. http://dx.doi.org/10.3390/fermentation9060556.

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The global production of fossil-based plastics has reached critical levels, and their substitution with bio-based polymers is an urgent requirement. Poly(3-hydroxybutyrate) (PHB) is a biopolymer that can be produced via microbial cultivation, but efficient microorganisms and low-cost substrates are required. Halomonas boliviensis LC1, a moderately halophilic bacterium, is an effective PHB producer, and hydrolysates of the residual stalks of quinoa (Chenopodium quinoa Willd.) can be considered a cheap source of sugars for microbial fermentation processes in quinoa-producing countries. In this s
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Blunt, Warren, Christopher Dartiailh, Richard Sparling, Daniel J. Gapes, David B. Levin, and Nazim Cicek. "Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46." Bioengineering 6, no. 4 (2019): 89. http://dx.doi.org/10.3390/bioengineering6040089.

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High cell density (HCD) fed-batch cultures are widely perceived as a requisite for high-productivity polyhydroxyalkanoate (PHA) cultivation processes. In this work, a reactive pulse feed strategy (based on real-time CO2 or dissolved oxygen (DO) measurements as feedback variables) was used to control an oxygen-limited fed-batch process for improved productivity of medium chain length (mcl-) PHAs synthesized by Pseudomonas putida LS46. Despite the onset of oxygen limitation half-way through the process (14 h post inoculation), 28.8 ± 3.9 g L−1 total biomass (with PHA content up to 61 ± 8% cell d
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Ruiz, Carolina, Shane T. Kenny, Ramesh Babu P, Meg Walsh, Tanja Narancic, and Kevin E. O’Connor. "High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440." Catalysts 9, no. 5 (2019): 468. http://dx.doi.org/10.3390/catal9050468.

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Waste cooking oil (WCO) is a major pollutant, primarily managed through incineration. The high cell density bioprocess developed here allows for better use of this valuable resource since it allows the conversion of WCO into biodegradable polymer polyhydroxyalkanoate (PHA). WCO was chemically hydrolysed to give rise to a mixture of fatty acids identical to the fatty acid composition of waste cooking oil. A feed strategy was developed to delay the stationary phase, and therefore achieve higher final biomass and biopolymer (PHA) productivity. In fed batch (pulse feeding) experiments Pseudomonas
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Gao, Ze-Liang, and You-Wei Cui. "Static Magnetic Field Increases Polyhydroxyalkanoates Biosynthesis in Haloferax mediterranei: Parameter Optimization and Mechanistic Insights from Metabolomics." Polymers 17, no. 9 (2025): 1190. https://doi.org/10.3390/polym17091190.

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Polyhydroxyalkanoates (PHAs), as biosynthetic and biodegradable polymers, serve as alternatives to petroleum-based plastics, yet face critical cost barriers in large-scale production. While magnetic field (MF) stimulation enhances microbial activity, the optimal MF parameters and metabolic mechanisms for PHA biosynthesis remain unexplored. This study optimized magnetic field parameters to increase PHA biosynthesis in Haloferax mediterranei. A custom-engineered electromagnetic system identified 110 mT of static magnetic field (SMF) as the optimal level for biosynthesis, reaching 77.97 mg/(L·h)
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Carvalho, João M., Bruno C. Marreiros, and Maria A. M. Reis. "Polyhydroxyalkanoates Production by Mixed Microbial Culture under High Salinity." Sustainability 14, no. 3 (2022): 1346. http://dx.doi.org/10.3390/su14031346.

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The fishing industry produces vast amounts of saline organic side streams that require adequate treatment and disposal. The bioconversion of saline resources into value-added products, such as biodegradable polyhydroxyalkanoates (PHAs), has not yet been fully explored. This study investigated PHA production by mixed microbial cultures under 30 gNaCl/L, the highest NaCl concentration reported for the acclimatization of a PHA-accumulating mixed microbial culture (MMC). The operational conditions used during the culture-selection stage resulted in an enriched PHA-accumulating culture dominated by
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Oehmen, Adrian, Fátima V. Pinto, Vera Silva, Maria G. E. Albuquerque, and Maria A. M. Reis. "The impact of pH control on the volumetric productivity of mixed culture PHA production from fermented molasses." Engineering in Life Sciences 14, no. 2 (2013): 143–52. http://dx.doi.org/10.1002/elsc.201200220.

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Dissertations / Theses on the topic "PHB volumetric productivity"

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MONGILI, BEATRICE. "Biotechnological approches for green-based bioplastic production." Doctoral thesis, Politecnico di Torino, 2020. http://hdl.handle.net/11583/2836776.

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