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Dissertations / Theses on the topic 'PhD domain'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Deeves, Sian Elizabeth. "Novel functions of the MOZ double PHD finger domain." Thesis, University of Nottingham, 2012. http://eprints.nottingham.ac.uk/12503/.

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Monocytic leukaemia zinc-finger protein (MOZ) is a histone acetyltransferase (HAT) implicated in haematopoiesis and acute myeloid leukaemia, as well as embryonic and postnatal development. MOZ contains multiple domains, including a MYST HAT domain and a double PHD finger domain (DPF) suggesting it interacts with histones. This work has established for the first time that the MOZ DPF exhibits dual functionality in establishing and sensing post-translational modifications (PTMs) of histones. Firstly, our data detected the direct interaction of MOZ with the N-terminal tails of histones H3 and H4 and shows that the MOZ DPF domain mediates such binding. Both PHD fingers are required and functionally cooperate to establish the DPF histone binding preference in terms of PTMs. We demonstrate that H3K4me3 prevents MOZ DPF association with H3, although H3K4me2 is tolerated. Similarly, H4Kac acts as a dominant exit signal that excludes MOZ from chromatin. This ability to sense H3K4 PTM status was confirmed in a collaborative effort establishing the crystal structure of MOZ DPF in complex with an unmodified H3 peptide. The H3 peptide adopted an α-helical conformation in the complex, which has not previously been observed. Secondly, we present novel data showing that the MOZ DPF domain exhibits a mild histone H3-specific acetyltransferase activity. This provides the first report of a possible enzymatic role in chromatin modification attributed to a PHD finger. Furthermore, the combined DPF and MYST domains were found to influence the reaction rate and substrate specificity of MOZ-induced histone acetylation. Our studies revealed that the MOZ DPF could associate with heterochromatic PTMs, namely H3K9me3. We report here that both the H3K9-specific methyltransferase SUV39H1 and heterochromatin protein 1 (HP1) form interactions with MOZ, implicating its function in both corepressor and coactivator complexes. Thus, our data suggest that like several other chromatin-associated proteins, MOZ is a multi-functional regulator of chromatin modification and gene expression.

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2

Delvecchio, Manuela. "Mécanisme de régulation de l'acétyltransférase p300/CBP." Phd thesis, Université de Grenoble, 2011. http://tel.archives-ouvertes.fr/tel-00631344.

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Le p300/CBP acétyltransférase est un co-activateur transcriptionnel très important qui est impliqué dans la régulation d'un grand nombre de processus biologiques, comme la transcription d'ADN, le développement et l'immunité innée. Jusqu'à présent, le rôle de p300/CBP dans la régulation de l'expression des gènes a été largement étudiée, mais les mécanismes qui régulent son activité enzymatique sont encore peu connus. Des études ont montré que le dysfonctionnement de p300/CBP est associé à plusieurs formes de cancer et de maladies neurodégénératives. Dés lors, chaque progrès concernant les mécanismes de régulation de p300/CBP est devenu primordial pour le développement de nouvelles thérapies. Le 'noyau' de p300/CBP contient deux domaines pour la reconnaissance des modifications post-traductionnelles (MPTs), un bromodomaine et un PHD finger (le module BP), adjacent à un domaine HAT (ou domaine histone acétyltransférase). Plusieurs enzymes, modifiant la chromatine, contiennent des domaines de reconnaissance des MPTs. Fréquemment des groupements particuliers de ces domaines sont très conservés et liés, au sein de la même protéine ou du même complexe protéique, suggérant qu'ils réalisent des fonctions coordonnées. Ces domaines adjacents peuvent agir en concertation dans la reconnaissance simultanée de différents MPTs ou peuvent exercer des fonctions différentes de celles qui sont effectuées par ces deux domaines particuliers, tels que les fonctions de régulation enzymatique. Plusieurs études suggèrent que les cycles acétylation/désacétylation dans la boucle d'auto-inhibition, à l'intérieur du domaine HAT, jouent un rôle important dans la régulation de l'activité enzymatique de p300/CBP. La proximité du module BP et du domaine HAT suggère que la spécificité de liaison, appartenant au module BP, peut être intrinsèquement liée à la régulation de l'activité du domaine HAT. L'objectif de ma thèse est de déterminer le rôle du module BP dans la régulation de l'activité du domaine HAT. Je propose que le module BP soit impliqué dans la régulation de p300/CBP de deux façons. La première consiste à établir un lien avec le domaine HAT qui stabilise la conformation auto-inhibée de l'enzyme. La deuxième exige que le module BP joue un rôle dans le choix des substrats de p300/CBP. J'ai été en mesure de montrer que BP peut se lier au domaine HAT et à la chromatine modifiée et qu'il peut reconnaître les modifications effectuées par p300/CBP lui-même. Les données obtenues indiquent que le module BP peut être impliqué dans la régulation de l'activité de p300/CBP et dans son ciblage à la chromatine.

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3

Wijeyekoon, Jananath Bhathiya. "Tissue expression and functional insights into HIF prolyl hydroxylase domain enzymes." Thesis, University of Oxford, 2013. http://ora.ox.ac.uk/objects/uuid:6330e4d5-0802-427b-a7b3-8895d94e02f2.

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This research programme investigated the expression of prolyl hydroxylase (PHD) proteins in rodent tissues. The importance of PHD enzymes lies in their ability to render oxygen sensitivity to Hypoxia inducible factor (HIF), the principal mediator of intracellular oxygen homeostasis. The first part of this study focused on developing and validating anti-sera capable of detecting PHD proteins in rodent tissues. With these reagents, it was possible to assess the relative expression of each PHD protein in a number of different rat tissues. PHD2 was the most abundant isoform in all tissues studied. In contrast, an abundance of PHD1 was observed only in testis and skeletal muscle. A number of different tissue species of PHD3 were identified and their abundance was found to vary between different tissues. These observations provide further evidence of the principal role of PHD2 in regulating HIF in vivo, but also point towards additional roles for PHD1 and PHD3 in selected tissues. They highlight the potential for there being a complex interplay between different PHD enzymes which could, in the future, prove potential targets for therapeutic manipulation. This study also provides additional insights into the mechanisms underlying the phenotypes observed in PHD deletional mouse models which appear, in many cases, to be directly related to the abundance of a given PHD isoform. The emerging role of PHD3 as a promoter of sympathetic lineage apoptosis prompted further study of PHD3 expression in rat neuronal tissues. An abundance of PHD3 was demonstrated throughout the rat sympathetic nervous system, a finding which appeared at odds with its known role as a promoter of neuronal apoptosis and resulted in a series of collaborative studies which demonstrated a sympatho-adrenal phenotype in wild type compared to PHD3-/- mice. Further collaborative studies utilising wild type mice and those deleted of specific PHD isoforms, were carried out to assess the significance of the abundance of PHD3 and PHD1 noted here in rat hippocampus and testis respectively. While neither study demonstrated statistically significant phenotypes, these observations remain of interest and areas for future research.

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4

Jiang, Yugang. "Large scale semantic concept detection, fusion, and selection for domain adaptive video search /." access full-text access abstract and table of contents, 2009. http://libweb.cityu.edu.hk/cgi-bin/ezdb/thesis.pl?phd-cs-b23749957f.pdf.

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Thesis (Ph.D.)--City University of Hong Kong, 2009.<br>"Submitted to Department of Computer Science in partial fulfillment of the requirements for the degree of Doctor of Philosophy." Includes bibliographical references (leaves 145-161)

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5

Mitra, Sayantan. "Arabidopsis Cohesin proteins: WAPL, CTF7 and PHD finger proteins: MMDL1, MMDL2 are essential for proper meiosis, gamete development and plant growth." Miami University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1517605898967702.

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6

Dhamodharan, Neelamegan. "Characterisation of PhdB, a pleckstrin homology domain containing protein in Dictyostelium discoideum." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972300252.

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7

Wang, Suqin. "Magnetization dynamics of single domain nanomagnets /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2007. http://uclibs.org/PID/11984.

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8

Hansen, Marc D. "An analysis of the diagrammatic visual data querying domain /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2005. http://uclibs.org/PID/11984.

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9

Holt-Martyn, James. "Novel and selective small molecule inhibitors and activators for the prolyl hydroxylase domain enzyme." Thesis, University of Oxford, 2018. http://ora.ox.ac.uk/objects/uuid:4b3613c5-5ff3-43b0-a07e-cf3116a37c1b.

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Hypoxia Inducible Factors (HIF) functions are master regulators of oxygen homeostasis and have a key role in the physiological responses to hypoxia including angiogenesis and erythropoiesis. Under hypoxia, levels of HIF-α subunits increase, they hetereodimerise with HIF-1β sub unit and promote the initiation of transcription of target genes. Under normoxia, oxygen dependent HIF-α degradation is promoted by hydroxylation of either of two proline residues (Pro402 and Pro564). The interaction of prolylhydroxylated HIF-α with the Von Hippel-Lindau protein (pVHL) promotes hydrolytic degradation of HIFα through an E3 ubiquitin ligase proteasomal pathway. HIF prolyl hydroxylation is catalysed by three 2-oxoglutarate (2OG)-dependent oxygenases known as prolyl hydroxylase domain (PHD 1-3) proteins, through an Fe(II) mediated catalytic process using 2OG, and oxygen. The PHDs are part of the family of Fe(II) bound 2OG dependent oxygenases. There are approximately 70 human 2OG oxygenases many of which have biologically important roles. Small-molecule inhibitors have reached advanced clinical trials; however, many clinical candidates inhibit other structurally similar 2OG oxygenases (OGFOD1 and vCPH) potentially altering the therapeutic effect. This thesis describes the design and synthesis of potent and 2OG oxygenase selective inhibitors for the PHDs. The 1,3,8-triazaspiro[4.5]decane-2,4-dione and 4-hydroxy-2-(pyrazole)pyrimidine-5-amide series were chosen as initial 'hits' (reported in the patent literature). The main analogues of the series were characterised in vitro and in cells as potent and selective PHD inhibitors over structurally similar 2OG oxygenases (Chapter 2). Broad structure activity relationship (SAR) of both initial series demonstrated the sensitivity for PHD2 inhibition (Chapter 3). Combination of SAR work described in Chapters 2 and 3 lead to the development of the novel 4-hydroxy pyridine series. In-depth SAR resulted in optimised analogues including 1 (IC50 69 nM) and highly selective over structurally similar 2OG oxygenases including OGFOD1. The completed SAR work led to the development of two novel pharmacophores 2 and 3. Both pharmacophores displayed potent PHD inhibition and selectivity over OGFOD1. Analogues including 1 and 3 displayed on target cellular activity stabilising HIF-1α at 20 μM (Chapter 4). The 4-dimethylamine pyridine analogue displayed an increase in substrate hydroxylation on PHD2 in contrast to the DMSO control (Chapter 3). SAR and cellular characterisation indicated that the effect observed was not an assay artifact (Chapter 5). Fenofibrate was used as a starting point for the development of novel inhibitors of the oxygen consumption rate (OCR) via mitochondrial inhibition (Chapter 6). Analogues were synthesised in order to conduct broad SAR and on-target cellular activity was observed in a Seahorse XF assay (50% reduction in the OCR at 1 μM). A selection of amino and amide analogues warrant further investigation.

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10

Słowiński, Witold. "Autonomous learning of domain models from probability distribution clusters." Thesis, University of Aberdeen, 2014. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=211059.

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Nontrivial domains can be difficult to understand and the task of encoding a model of such a domain can be difficult for a human expert, which is one of the fundamental problems of knowledge acquisition. Model learning provides a way to address this problem by allowing a predictive model of the domain's dynamics to be learnt algorithmically, without human supervision. Such models can provide insight about the domain to a human or aid in automated planning or reinforcement learning. This dissertation addresses the problem of how to learn a model of a continuous, dynamic domain, from sensory observations, through the discretisation of its continuous state space. The learning process is unsupervised in that there are no predefined goals, and it assumes no prior knowledge of the environment. Its outcome is a model consisting of a set of predictive cause-and-effect rules which describe changes in related variables over brief periods of time. We present a novel method for learning such a model, which is centred around the idea of discretising the state space by identifying clusters of uniform density in the probability density function of variables, which correspond to meaningful features of the state space. We show that using this method it is possible to learn models exhibiting predictive power. Secondly, we show that applying this discretisation process to two-dimensional vector variables in addition to scalar variables yields a better model than only applying it to scalar variables and we describe novel algorithms and data structures for discretising one- and two-dimensional spaces from observations. Finally, we demonstrate that this method can be useful for planning or decision making in some domains where the state space exhibits stable regions of high probability and transitional regions of lesser probability. We provide evidence for these claims by evaluating the model learning algorithm in two dynamic, continuous domains involving simulated physics: the OpenArena computer game and a two-dimensional simulation of a bouncing ball falling onto uneven terrain.

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11

Ren, Beibei. "A domain-specific cell based asic design methodology for digital signal processing applications /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2005. http://uclibs.org/PID/11984.

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12

Leidy, Chad. "Thermotropic behavior of lipid domains in model membranes /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2002. http://uclibs.org/PID/11984.

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13

Chen, Lili. "Elucidation of functional domains of ryanodine receptor complexes /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2002. http://uclibs.org/PID/11984.

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14

Wilkinson, Debbie Isabelle. "Visualisation of osteoclast membrane domains." Thesis, University of Aberdeen, 2010. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158808.

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Osteoclasts polarise upon activation and form four distinct membrane domains; the basolateral domain, the sealing zone, the functional secretory domain and the ruffled border. The ruffled border is the resorptive organelle of the cell and provides a large surface area for the release of protons and enzymes into the space beneath the osteoclast. Defects in osteoclast formation or function can lead to diseases such as osteopetrosis. Ruffled border formation is a critical event in osteoclast function but the process by which it and other membrane domains form is only partially understood. Vesicular trafficking is essential for the tight regulation of the osteoclast membrane domains and it has been shown previously that treatment with pharmacological inhibitors causes disruption of trafficking. The aims of this PhD were to increase our understanding of vesicular trafficking in osteoclasts and to optimise ways of visualising osteoclast membrane domains. My studies of patients with osteoclast-poor osteopetrosis identified defects in RANKL as a cause of the defect. This in turn has identified a potential therapy of recombinant RANKL for patients with this form of the disease. Although purification of wild type or mutant RANKL was not completely successful, it did suggest that the mutant forms of RANKL were not functional. I have used pharmacological inhibitors to study osteoclast membrane domains, and found that transmission electron microscopy is an essential tool for studying membrane changes following pharmacological inhibition at the ultrastructural level. I also established that the study of vesicular trafficking to analyse formation of membrane domains can make excellent use of immuno-electron methods. Furthermore, genetic diseases associated with defective ruffled border formation such as XLA and osteopetrosis provide useful tools to further analyse the dynamics involved in the formation and maintenance of the ruffled border, as well as revealing more about the diseases themselves.

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15

Chen, Qin. "Time domain dielectric microwave detection of biomolecular surface interactions with a coplanar transmission line probe /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.

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16

Torcedo, Jojit Camama. "Time-domain Terahertz Spectroscopy of water." Diss., [Riverside, Calif.] : University of California, Riverside, 2010. http://proquest.umi.com/pqdweb?index=0&did=2019861181&SrchMode=2&sid=1&Fmt=2&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1274284155&clientId=48051.

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Thesis (Ph. D.)--University of California, Riverside, 2010.<br>Includes abstract. Title from first page of PDF file (viewed May 18, 2010). Includes bibliographical references. Issued in print and online. Available via ProQuest Digital Dissertations.

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17

Rieben, Robert N. "A novel high order time domain vector finite element method for the simulation of electromagnetic devices /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2004. http://uclibs.org/PID/11984.

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18

Kang, Meghan. "Eminent domain a case study of Coatesville /." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 80 p, 2007. http://proquest.umi.com/pqdweb?did=1253511381&sid=1&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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19

Purkayastha, Pratik. "Diagnostics and Prognostics of safety critical systems using machine learning, time and frequency domain analysis." Thesis, Blekinge Tekniska Högskola, Institutionen för tillämpad signalbehandling, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:bth-17603.

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The prime focus of this thesis was to develop a robust Prognostic and Diagnostic Health Management module (PDHM), capable of detecting faults, classifying faults, fault progression tracking and estimating time to failure. Priority was to obtain as much accuracy as possible with the bare minimum amount of sensors as possible. Algorithms like k-Nearest Neighbors (k-NN), Linear and Non- Linear regression and development of rule engine to identify safe operating limits were deployed. The entire solution was developed using R (v 3.5.0). The accuracy of around 98% was obtained in diagnostics. For Prognostics, our ability to predict time to failure more accurately increases with time. Some balance must be there between learning horizon and predicting horizon in order to get good predictions with reasonable time left to hit catastrophic failure. In conclusion, the PDHM module works just as desired and makes Predictive maintenance, smart replacement and crisis prediction possible ensuring the safety and security of people on board and assets.

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20

Li, Jian. "Theory and application methods of time domain reflectometry/time domain transmission computed tomography (TDR/TDT CT)." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, p, 2007. http://proquest.umi.com/pqdweb?did=1397912601&sid=5&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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21

Lowenthal, Andrew Charles. "Analysis of ancillary homology domains within the Heliobacter pylori chemotaxis pathway /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2007. http://uclibs.org/PID/11984.

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22

Sun, Xiantang. "Domain independent generation from RDF instance date." Thesis, Available from the University of Aberdeen Library and Historic Collections Digital Resources, 2008. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24972.

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23

Fuller, Patrick Michael. "Time domains of the neurovestibular system : from reflex circuits to circadian rhythms /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2004. http://uclibs.org/PID/11984.

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Ekkerman, Silvia. "The role of KTN domains in potassium homeostasis." Thesis, University of Aberdeen, 2016. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=229706.

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Potassium ions are the most abundant cation and potassium transport is essential in maintaining cellular homeostasis through the regulation of cell turgor and cytoplasmic pH. It allows bacteria to grow and survive, therefore, the potassium pool needs to be strictly controlled, which is mainly performed by transport systems that contain a KTN domain. The potassium efflux system, Kef, is such a KTN-bearing system and it is widespread among Gram negative bacteria. The system provides protection against harmful electrophiles through cytoplasmic acidification. Kef is a glutathione-regulated protein: it is inhibited by glutathione (GSH), but it becomes activated by binding glutathione-S-conjugates (GSX), that are formed in the presence of electrophiles. GSH or GSX are bound in the same pocket that is located in a cytosolic regulatory domain which controls the K+ flux. Previous studies already showed that bacterial growth is inhibited when the gating of Kef is manipulated, which makes Kef a potential target for developing novel antibacterial drugs. Structure-Function studies have already lead to a better understanding of the regulation of potassium efflux activity, but no quantitative analyses had been performed until now. A simplified model Kef system (SdKef) is presented and a novel assay was developed that provided new insights into the structural components necessary for the gating of Kef. This assay makes the search for modulators of Kef, and therefore potential novel antibacterial drugs, more easily accessible. Another objective was to identify the nucleotide(s) bound and to determine its role in controlling the Kef system. This nucleotide was identified as AMP which is essential for stability of the Kef system.

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Pasko, Jaroslaw Piotr. "RCK domains of potassium uptake systems, Trk and CglK." Thesis, University of Aberdeen, 2013. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=210109.

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RCK (regulating the conductance of K+) domains are ubiquitous among a wide variety of cation translocation systems or channels. Whether it is inward or outward transport, RCK domains share high sequence similarity from protein to protein, suggesting that they perform important functions in these systems. Although specific functions are not yet fully understood, RCK domains bind nucleotides, a characteristic that has been suggested to be important for the open/closed transition. In some cases RCK domains can bind additional ligands, e.g. KefC binding to glutathione. This project provides an in-depth study of two equally important bacterial potassium uptake systems, Trk (from E. coli) and CglK (from C. glutamicum). In these systems, RCK domains form octamers that either are anchored (CglK) or are separate and bind to transmembrane partners (Trk). The overall objective of this study was to examine the ligand control of the Trk potassium uptake system, including ligand identification and binding effects on its conformation, and therefore activity control. A crystal structure of the Trk potassium uptake system from Vibrio parahaemolyticus, was published recently [1] and provided evidence of ADP/ATP switch as a control mechanism. In accordance with the aforementioned study, the work presented in this thesis provides strong evidence that both ADP and ATP can bind to E. coli TrkA. Furthermore, it was shown that NADH bind strongly to one of the two RCK domains present in TrkA protein. The data presented here suggest a more complex control mechanism of the E. coli Trk system. CglK is a major potassium uptake system of C. glutamicum, but little is known about its control mechanism. The mutagenesis approach was undertaken to learn more about the system and its underlying processes/mechanisms. The work shown in this thesis indicates a similarity of CglK to other potassium channels, such as MthK and GsuK. The functional mechanisms proposed for those two systems were proven to be similar to that of CglK, although the specific CglK activation ligands are still to be found.

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Toniolo, Alice. "Models of argument for deliberative dialogue in complex domains." Thesis, University of Aberdeen, 2013. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=201686.

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In dynamic multiagent systems, self-motivated agents pursuing individual goals may interfere with each other's plans. Agents must, therefore, coordinate their plans to resolve dependencies among them. This drives the need for agents to engage in dialogue to decide what to do in collaboration. Agreeing what to do is a complex activity, however, when agents come to an encounter with different objectives and norm expectations (i.e. societal norms that constrain acceptable behaviour). Argumentation-based models of dialogue support agents in deciding what to do analysing pros/cons for decisions, and enable conflict resolution by revealing structured background information that facilitates the identification of acceptable solutions. Existing models of deliberative dialogue, however, commonly assume that agents have a shared goal, and to date their effectiveness has been shown only through the use of extended examples. In this research, we propose a novel model of argumentation schemes to be integrated in a dialogue for the identification of plan, goal and norm conflicts when agents have individual but interdependent objectives. We empirically evaluate our model within a dynamic system to establish how the information shared with argumentation schemes influence dialogue outcomes. We show that by employing our model of arguments in dialogue, agents achieve more successful agreements. The resolution of conflicts and identification of more feasible interdependent plans is achieved through the sharing of focussed information driven by argumentation schemes. Agents may also consider more important conflicts, or conflicts that cause higher loss of utility if unresolved. We explore the use of strategies for agents to select arguments that are more likely to solve important conflicts.

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Dooley, Helen. "Characterisation of single domain antibody fragments from the nurse shark Ginglymostoma cirratum, using phage display." Thesis, University of Aberdeen, 2001. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=219953.

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Silvagni, Paul Anthony. "Comparative pathology and diagnosis of domoic acid toxicity /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2003. http://uclibs.org/PID/11984.

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AbuGhazaleh, Shereen N. A. "The resolution of domain name disputes : a comparison of Jordan, United Kingdom, United States, and ICANN rules." Thesis, University of Aberdeen, 2010. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158341.

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Domain names constitute a valuable key element in electronic commerce. However, some intend to benefit from this fact by registering identical or similar trademarks as domain names, thereby depriving the legitimate owners from obtaining the domain name. This thesis aims to evaluate the protection provided for domain names, by illustrating several models that are under civil, common and international jurisdiction, namely, Jordan, the United Kingdom, the United States and by ICANN. In the first model there are no special regulations. In the second model alternative regulations are applied while, in the third model, a special act is provided; the fourth model constitutes a global protection for specific types of domain name disputes. It is concluded that the absence of a unified legal identity for domain names is the reason why diverse positions are required to protect them; concomitantly, domain name disputes have not been sufficiently addressed, and there is a necessity to provide domain names with a globally comprehensive protection mechanism.

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Koester, Oliver. "Building domain and information source models for information agents /." Available to subscribers only, 2007. http://proquest.umi.com/pqdweb?did=1328068731&sid=13&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Donziger, Alan J. "Property rights the issue of eminent domain, a legal and constitutional analysis /." Click here for download, 2007. http://proquest.umi.com/pqdweb?did=1276419901&sid=1&Fmt=2&clientId=3260&RQT=309&VName=PQD.

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Monaghan, Amy Elizabeth. "The amino terminal domain of steroid hormone receptors as a novel drug target : identification of small molecule inhibitors." Thesis, University of Aberdeen, 2016. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230709.

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Steroid hormone receptors (SHRs) are well validated therapeutic targets in a number of diseases. Current therapies competitively antagonise the ligand binding domain (LBD), blocking activation of the receptor and downstream signalling pathways. However cross-reactivity can be seen amongst the antagonists of different SHRs eliciting unwanted side effects. Additionally the acquisition of resistance to current therapies in diseases such as prostate cancer limits their use. The amino-terminal domain (NTD) of SHRs provides an alternative target for antagonism by allowing potential therapies to block receptor transactivation and inhibit interactions with co-activator proteins. Reduced homology between different SHR NTDs also increases the specificity of drug interactions. However development of targeted therapies using rational drug design has been hindered by its intrinsically disordered structure. Establishing cell lines which stably express a SHR responsive reporter gene alongside variants of SHRs lacking the LBD provides a method by which small molecules specifically targeting the NTD of each receptor can be identified. This assay has been designed to overcome the barriers to drug discovery that are presented by an intrinsically disordered protein. The project follows the design, development, optimisation and implementation of a high throughput screening assay with the potential to identify novel small molecule inhibitors of SHRs. The applications of these inhibitors are highlighted throughout, with specific reference to their potential to inhibit the androgen receptor in prostate cancer.

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Ricker, Josette Valerie. "Effects of trehalose on phase behavior of dry lipid mixtures : implications for stability of domains in dried membranes /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2003. http://uclibs.org/PID/11984.

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Wang, Zhongxiao. "Parallel computation for reservoir thermal simulation An overlapping domain decomposition approach /." Ann Arbor, Mich. : Proquest, 2005. http://proquest.umi.com/pqdweb?index=0&did=954046251&SrchMode=1&sid=1&Fmt=2&VInst=PROD&VType=PQD&RQT=309&VName=PQD&TS=1187901937&clientId=57025.

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Syed, Azeemuddin. "Permalloy magnetic domain control for radio frequency integrated circuit applications /." Available to subscribers only, 2005. http://proquest.umi.com/pqdweb?did=1079666501&sid=10&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Kim, Ikjin. "Roles of UBL domain containing proteins in proteolysis : a dissertation /." San Antonio : UTHSC, 2007. http://proquest.umi.com.libproxy.uthscsa.edu/pqdweb?did=1490071661&sid=4&Fmt=2&clientId=70986&RQT=309&VName=PQD.

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Orafidiya, Folake. "Disrupting protein-protein interactions in the amino-terminal domain of the androgen receptor : design, characterization and effects of peptide inhibitors." Thesis, University of Aberdeen, 2015. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=228208.

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Mallia, Joseph. "Business Transformation Enablement Program : understanding enterprise architecture adoption in the European National Banking Domain through the Business Transformation Enablement Program." Thesis, University of Aberdeen, 2016. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=230158.

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This research examines WHY and HOW a new approach to Enterprise Strategy and IT planning can be adopted in the European national financial sector by using Enterprise Architecture (EA). EA has been promoted as a key tool for transformation and modernisation of the enterprise. By following best practices from successful case studies and the European Central Bank (ECB), the claim is that the adoption of EA will ensure that IT resources and business processes are planned, leveraged, and coordinated better in national financial sectors. In our five supporting papers, several qualitative case studies in Europe were investigated by applying an interpretive perspective. According to common belief EA connects business and IT; it is an important tool for survival and growth. Many EA endeavours, however, fail. The question is how can risks of EA implementation failure be reduced at an early stage? Underlying questions concern: why would a particular organisation need EA, can implementation risks be identified and are solutions available to reduce the failure rate? In order to answer research questions a literature study is performed to build the Business Transformation Enablement Program framework (BTEP). It advocates deliberate motivation, active risk management and an iterative and incremental implementation of EA. Next to the literature, the framework is validated through the Small National Bank (SNB) implementation. Data was collected in both structured, semi-structured and ad-hoc methods. Grounded theory techniques were used to analyse the data logically, using existing theory only as prior constructs. The theoretical abstractions, generalisations and experience generated in the research process have been published and availed to specialised groups.

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Wagner, Jennifer Ann. "The S. cerevisiae dynamin related GTPase, Mgm1p, is required to maintain inner mitochondrial membrane structure and mutations in Mgm1p's predicted GTPase domain and GED result in abnormal mitochondrial morphology /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2002. http://uclibs.org/PID/11984.

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Armstrong, Howard Meredith Dana Armstrong Howard Meredith Dana. "Harmful algal blooms on the U.S. west coast : new insights into domoic acid production and identification of yessotoxin, a new marine toxin detected in California coastal waters /." Digital Dissertations Database. Restricted to UC campuses, 2007. http://uclibs.org/PID/11984.

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Buschhaus, Magdalena J. "Isolation of highly selective VNAR domains to SEED bispecific antibody scaffold for bioprocessing applications." Thesis, University of Aberdeen, 2018. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=236928.

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Glancy, Paul Michael. "Terahertz time domain spectroscopy (THz-TDS) of hydrated biomolecular polymers and monomers." Diss., UC access only, 2009. http://proquest.umi.com/pqdweb?index=153&did=1906549291&SrchMode=1&sid=1&Fmt=7&retrieveGroup=0&VType=PQD&VInst=PROD&RQT=309&VName=PQD&TS=1270496277&clientId=48051.

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Thesis (Ph. D.)--University of California, Riverside, 2009.<br>Vita. Includes abstract. Includes bibliographical references (leaves 148-155). Issued in print and online. Available via ProQuest Digital Dissertations.

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Plotkowski, Megan Lynn. "Characterization and modulation of transmembrane domain interactions in membrane protein drug-targets." Diss., Restricted to subscribing institutions, 2008. http://proquest.umi.com/pqdweb?did=1666392021&sid=2&Fmt=2&clientId=1564&RQT=309&VName=PQD.

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Fala, Angela Maria 1983. "Expressão e purificação heteróloga do fator de transcrição induzido por hipóxia HIF-1 humano visando estudos estruturais e bioquímicos e estudos estruturais das prolil-hidroxilases (PHDs) humanas, isoformas 1 e 3, em complexo com inibidores." [s.n.], 2013. http://repositorio.unicamp.br/jspui/handle/REPOSIP/308129.

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Orientador: Andre Luís Berteli Ambrósio<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas<br>Made available in DSpace on 2018-08-23T16:57:05Z (GMT). No. of bitstreams: 1 Fala_AngelaMaria_M.pdf: 4666864 bytes, checksum: 3bcb145c1dc353b15b43b75e5723a310 (MD5) Previous issue date: 2013<br>Resumo: A adaptação das células cancerosas ao microambiente é o ponto central que leva ao fenótipo invasivo e metastático, e é garantida principalmente através do controle preciso da expressão gênica. A resposta às necessidades energéticas e biossintéticas e principalmente à disponibilidade de oxigênio intracelular, por exemplo, é em grande parte mediada pelo fator de transcrição induzido por hipóxia 1 (HIF-1). HIF-1 é um heterodímero composto pelas subunidades ? e ?, que respondem a sequência consenso (5'-RCGTG-3') e ativam a transcrição de mais de 100 genes envolvidos em diversos aspectos cruciais da biologia tumoral, incluindo angiogênese, metabolismo de glicose, diferenciação celular, apoptose e resistência a radio e quimioterapias. São conhecidas três isoformas da subunidade ? (1 a 3) e todas se heterodimerizam com a subunidade ?. No geral, HIFs são constituídas de diferentes domínios funcionais, como de ligação ao DNA, de heterodimerização, transativação e degradação. Atualmente, pouco se sabe sobre os mecanismos estruturais e funcionais dos domínios da HIF- 1, deste modo este trabalho objetivou o estudo estrutural destes domínios. Os domínios bHLH, Pas-1 e Pac de HIF-1? e HIF-? em diferentes combinações entre si e o domínio Pac da HIF-3? foram clonados, as proteínas foram expressas em sistema bacteriano e purificadas por diferentes técnicas cromatográficas. Diversas destas construções se mostraram insolúveis ou suscetíveis a degradação, enquanto outras foram purificadas com sucesso. As construções Pac, um exemplo de sucesso na produção, foram submetidas a ensaios de anisotropia de fluorescência e ressonância magnética nuclear, o que nos permitiu a caracterização dos perfis de interação entre as várias combinações de heterodimerização. Neste contexto, os resultados mostram que o equilíbrio dinâmico da interação entre Pac-1? com a subunidade -1? é alcançado imediatamente, enquanto que para a interação entre Pac-3? e -1?, são necessários pelo menos 30 horas de incubação. O mesmo pode ser extraído da caracterização da interação direta entre Pac-1? e Pac-3?. Nos experimentos de RMN, foi possível identificar a região de interação entre as subunidades -1? e -3? com a subunidade ?, separadamente. Ambas as subunidades ? interagem com a Pac-1? na região das fitas-beta 1 e 5 e no loop entre as fitas 4 e 5. Em conjunto, estes resultados impactam no mecanismo de antagonização de HIF-3? na atividade transcricional de HIF-1?. Houve ainda a formação de monocristais da subunidade Pac-3?, que foram submetidos a experimentos preliminares de difração de raios X, que apesar de resultar em dados anisotrópicos e insuficientes para resolução estrutural, permitiram a caracterização dos parâmetros cristalinos, incluindo a presença de um alto conteúdo de solvente. Adicionalmente, são também apresentados os resultados obtidos visando a expressão e cristalização das Prolilhidroxilases (PHDs) isoformas 1 a 4, durante estágio de seis meses no Structural Genomics Consortium (SGC), da Universidade de Oxford, na Inglaterra. Foram expressas de maneira solúvel e purificadas, diversas construções das isoformas 1 e 3 das PHDs humanas. Cristais foram obtidos, porém estes foram determinados como sendo de compostos inorgânicos presentes na condição de cristalização. Como resultado final, está sendo estabelecida uma colaboração entre o nosso grupo e o SGC para que os estudos estruturais com PHDs se estendam e sejam realizados em nosso laboratório aqui no Brasil<br>Abstract: The adaptation process of cancer cells to the microenvironment is the central point leading to the invasive and metastatic phenotypes, and is guaranteed mainly through the precise control of gene expression. The cell response to the energetic and biosynthetic needs and especially to the availability of intracellular oxygen is mediated by the hypoxia inducible transcription factor 1, or HIF-1. HIF-1 functions as a heterodimer composed by subunits ? and ?, binding to responsive elements with the consensus sequence 5'-RCGTG-3 ', thus activating the transcription of more than 100 genes involved in many crucial aspects of tumor biology, including angiogenesis, metabolism glucose, cell differentiation, apoptosis, and resistance to radiotherapy and chemotherapy. There are three known isoforms of the ? subunit (1, 2 and 3) and all heterodimerize with the ? subunit. HIFs are composed of different functional domains, such as the DNA binding domain, the heterodimerization, transactivation and the oxygen-dependent degradation domains. Currently, little is known about the mechanisms of structural and functional domains of HIF-1, thus this work was to study these structural domains. The domain (bHLH, Pas-1 and Pac) of HIF-1? and HIF-? in different combinations with each other and Pac domain of HIF-3? were cloned, the proteins were expressed in bacterial system and purified by various chromatographic techniques. Several of these constructs proved insoluble or susceptible to degradation, while others were purified successfully. The constructs Pac, an example of success in production, were tested for fluorescence anisotropy and nuclear magnetic resonance, which allowed us to characterize the profiles of the interaction between the various combinations of heterodimers. In this context, the results show that the dynamic equilibrium of the interaction between the Pac-1? and -1? subunits is achieved immediately, whereas for the interaction between Pac-3? and -1?, it takes at least 30 hours of incubation. The same can be observed from the characterization of direct interaction between Pac-1? and -3?. From the NMR experiments, it was possible to identify the region of interaction between the subunits -1? and -3? with the -1? subunit. Both ? subunits interact with Pac-1? via betastrands 1 and 5 and the loop between the strands 4 and 5. Overall, these results impact in the mechanism of HIF-3? antagonizing the transcriptional activity of HIF-1?. We also obtained single crystals for Pac-3? subunit, which were subjected to preliminary experiments of X-ray diffraction. Although resulting in anisotropic, insufficient data for and structural resolution, it has allowed the characterization of crystalline parameters, including the presence of a high solvent content. Additionally, we also present the results targeting the expression and crystallization of Prolyl-hydroxylases (PHDs) human isoforms 1-4, during the six-month period at the Structural Genomics Consortium (SGC), the University of Oxford in England. Several construct from of isoforms 1 and 3 were successfully expressed and purified in the soluble form. Likewise, crystals were obtained, but these were determined to be composed by inorganic compounds present in the crystallization conditions. At the end, a collaboration was established between our and the SGC group for the structural studies with the PHDs to extend and carry out the experiments in our lab here in Brazil<br>Mestrado<br>Clinica Medica<br>Mestra em Clínica Médica

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45

Xu, Chao. "NMR structural studies of apolipoprotein E amino-terminal domain and amyloid beta peptide /." Available to subscribers only, 2006. http://proquest.umi.com/pqdweb?did=1136079871&sid=15&Fmt=2&clientId=1509&RQT=309&VName=PQD.

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Thesis (M.S.)--Southern Illinois University Carbondale, 2006.<br>"Department of Molecular Biology, Microbiology and Biochemisty." Includes bibliographical references (leaves 105-113). Also available online.

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Price, Daniel Kenneth. "Development of an accelerated finite-difference time-domain solver using modern graphics processors." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 111 p, 2009. http://proquest.umi.com/pqdweb?did=1654487621&sid=4&Fmt=2&clientId=8331&RQT=309&VName=PQD.

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Hoch, Tomáš. "Přístupy a praktiky k vývoji současných webových aplikací - realizace webové platformy pro tvorbu online vzdělávacích kurzů." Master's thesis, Vysoká škola ekonomická v Praze, 2016. http://www.nusl.cz/ntk/nusl-203985.

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This thesis deals with all web application development phases, analyses key activities and common issues of each phase and shows suitable principals and practices used to solve those issues. Described principals and practices do not only solve partial development problems just as isolated segments, but also takes into account overall web application solution. This thesis targets mostly junior developers, who through it might connect to their theoretical knowledge gained during studies and also gain knowledge representing the base knowledge of each developer. This thesis includes examples, findings and benefits resulting from an application of stated principals and practices during Leduvio platform development.

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Fischer, Katharina. "The mineralocorticoid receptor amino terminal transactivation domain investigation of structural plasticity and protein-protein interactions /." Thesis, Available from the University of Aberdeen Library & Historic Collections Digital Resources, 2008. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=24694.

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Thesis (Ph.D.)--Aberdeen University, 2008.<br>Title from web page (viewed on Feb. 23, 2009). With: Natural disordered sequences in the amino terminal domain of nuclear receptors : lessons from the androgen and glucocorticoid receptors / Iain J. McEwan ... et al. Nuclear Receptor Signalling. 2007: 5. Includes bibliographical references.

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Gardner, Stewart G. "Studies of PhoU in Escherichia coli: Metal Binding, Dimerization,Protein/Protein Interactions, and a Signaling Complex Model." BYU ScholarsArchive, 2014. https://scholarsarchive.byu.edu/etd/5685.

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Phosphate is an essential nutrient for all forms of life. Escherichia coli has a PhoR/PhoB two component regulatory system that controls the expression of various genes whose products allow the cell to thrive in low phosphate environments. The signaling mechanism of the PhoR/PhoB system has been studied and the phosphorylation cascade that controls gene expression is well understood. What is still unknown is how PhoR senses the phosphate level of the environment. The PstS, PstC, PstA, PstB, and PhoU proteins play a role in this signal sensing. This work confirms the hypothesis that the PstSCAB complex senses the environmental phosphate and that phosphate signal is passed through PhoU to PhoR. Further, this work characterizes residues important for interaction on PhoU and PhoR and identifies a structural model for interaction. This model points to a potential mechanism for PhoU mediated signaling to PhoR. We tested this model with direct coupling analysis and obtained further confirmation. Further use of these techniques may elucidate more of the interactions necessary for proper phosphate signaling.

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Qian, Yi. "Flipping a MAGUK switch : complex domain interactions regulating ligand binding to the tumor suppressor Dlg /." view abstract or download file of text, 2006. http://proquest.umi.com/pqdweb?did=1251819311&sid=1&Fmt=2&clientId=11238&RQT=309&VName=PQD.

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Thesis (Ph. D.)--University of Oregon, 2006.<br>Typescript. Includes vita and abstract. Includes bibliographical references (leaves 68-71). Also available for download via the World Wide Web; free to University of Oregon users.

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