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Journal articles on the topic 'Phenolic phytochemicals'

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1

Taulavuori, Kari, Riitta Julkunen-Tiitto, Valtteri Hyöky, and Erja Taulavuori. "Blue Mood for Superfood." Natural Product Communications 8, no. 6 (June 2013): 1934578X1300800. http://dx.doi.org/10.1177/1934578x1300800627.

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The term superfood refers to food with high levels of either nutrient or bioactive phytochemicals with human health benefits. Phytochemicals are naturally occurring compounds in plants that provide their color, flavor and odor. Phenolic compounds form the major constituents of phytochemicals. Plant traits in phytochemical production are tightly bound with the genome while modified markedly by the environmental conditions. Here, we studied the effect of supplemented blue light on the production of several phenolics in the leaves of tomato, basil and parsley, which are widely cultivated food plant species. The results indicated doubled or higher increases in the accumulation of several species-specific phenolic acids or flavonoids. In conclusion, we showed for the first time, that supplemented blue light results in high yield improvement of phytochemicals related to superfood products.
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Mascarenhas, Maria Emilia, Cibani Ramesh Mandrekar, Pratiksha Bharat Marathe, and Luena Joey Morais. "PHYTOCHEMICAL SCREENING OF SELECTED SPECIES FROM CONVOLVULACEAE." International Journal of Current Pharmaceutical Research 9, no. 6 (November 14, 2017): 94. http://dx.doi.org/10.22159/ijcpr.2017v9i6.23438.

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Objective: To screen phytochemicals from stems and leaves of 23 selected taxa of Convolvulaceae.Methods: Stem and leaves of the selected 23 taxa were collected and shade dried. Methanol extract was used for preliminary screening of phytochemicals such as alkaloids, carbohydrates, glycosides, saponins, proteins, phytosterols, tepenoids, fixed oils, phenolic compounds, flavonoids and tannins.Results: Phytochemicals were present in all selected taxa of Convolvulaceae. Leaves showed most of the phytochemicals than stems. The important phytochemicals in leaves were flavonoids, carbohydrates, alkaloids, saponins, tannins and phenolic compounds while those in stems were carbohydrates, saponins and phenolic compounds. Proteins and fixed oils were absent in the taxa studied.Conclusion: Phytochemical screening in the present study, revealed that maximum phytochemicals were present in leaves than in stems.
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3

Sharma, Swati. "PHYTOCHEMICAL SCREENING AND QUANTITATIVE ESTIMATION OF TOTAL PHENOLIC CONTENT AND TOTAL FLAVONOID CONTENT OF GRAINS OF PASPALUM SCROBICULATUM." Asian Journal of Pharmaceutical and Clinical Research 9, no. 6 (November 1, 2016): 73. http://dx.doi.org/10.22159/ajpcr.2016.v9i6.13552.

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Paspalum scrobiculatum, commonly known as ‘Kodo’ is a millet variety found in India having medicinal and pharmaceutical importance. Crude extracts of chloroform, ethyl acetate, ethanol and methanol were evaluated for the presence of total phenolic content and total flavonoid content of the millet grains. Phytochemical screening was done for a qualitative basis of the study. Phytochemical tests showed the presence of phenolics and flavonoids in more polar solvent, methanol and ethanol. Carbohydrates and fats and oils were present in all four extracts. The result of total phenolic content by Folin- ciocalteu method with gallic acid as standard at 765 nm ranged from 40.15 ± 0.17 to 175.94 ± 1.018 µg/100µg extract. Ethanol extract showed maximum phenolic content. The total flavonoid content at 510 nm using rutin as standard ranged from 4.88 ± 0.50 to 116.48 ± 1.57 µg/100µg extract. Highest flavonoid content was found in ethanol extract. Presence of good amount of phenolic and flavonoid content indicates presence of phytochemicals in kodo millet having good antioxidant potential.Keywords: Paspalum scrobiculatum, Phytochemical, TPC, TFC, Ethanol, etc.
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4

Habauzit, Véronique, and Marie-Noëlle Horcajada. "Phenolic phytochemicals and bone." Phytochemistry Reviews 7, no. 2 (November 21, 2007): 313–44. http://dx.doi.org/10.1007/s11101-007-9078-9.

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5

Bolling, Bradley W., C. Y. Oliver Chen, Diane L. McKay, and Jeffrey B. Blumberg. "Tree nut phytochemicals: composition, antioxidant capacity, bioactivity, impact factors. A systematic review of almonds, Brazils, cashews, hazelnuts, macadamias, pecans, pine nuts, pistachios and walnuts." Nutrition Research Reviews 24, no. 2 (December 2011): 244–75. http://dx.doi.org/10.1017/s095442241100014x.

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Tree nuts contain an array of phytochemicals including carotenoids, phenolic acids, phytosterols and polyphenolic compounds such as flavonoids, proanthocyanidins (PAC) and stilbenes, all of which are included in nutrient databases, as well as phytates, sphingolipids, alkylphenols and lignans, which are not. The phytochemical content of tree nuts can vary considerably by nut type, genotype, pre- and post-harvest conditions, as well as storage conditions. Genotype affects phenolic acids, flavonoids, stilbenes and phytosterols, but data are lacking for many other phytochemical classes. During the roasting process, tree nut isoflavones, flavanols and flavonols were found to be more resistant to heat than the anthocyanins, PAC and trans-resveratrol. The choice of solvents used for extracting polyphenols and phytosterols significantly affects their quantification, and studies validating these methods for tree nut phytochemicals are lacking. The phytochemicals found in tree nuts have been associated with antioxidant, anti-inflammatory, anti-proliferative, antiviral, chemopreventive and hypocholesterolaemic actions, all of which are known to affect the initiation and progression of several pathogenic processes. While tree nut phytochemicals are bioaccessible and bioavailable in humans, the number of intervention trials conducted to date is limited. The objectives of the present review are to summarise tree nut: (1) phytochemicals; (2) phytochemical content included in nutrient databases and current publications; (3) phytochemicals affected by pre- and post-harvest conditions and analytical methodology; and (4) bioactivity and health benefits in humans.
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Fang, Haitian, Huiling Zhang, Xiaobo Wei, Xingqian Ye, and Jinhu Tian. "Phytochemicals and Antioxidant Capacities of Young Citrus Fruits Cultivated in China." Molecules 27, no. 16 (August 15, 2022): 5185. http://dx.doi.org/10.3390/molecules27165185.

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Fruits of six varieties of young citrus cultivated in China were collected for phytochemical composition analysis and antioxidant activity determination. The phenolic acids, synephrine, flavone, and flavanone were analyzed using HPLC, and the total phenolic content and antioxidant capacity were determined by Folin-Ciocalteu, Ferric ion reducing antioxidant power (FRAP), 2,2- 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) analysis. The results indicated that Ougan variety had the highest total phenolic content (125.18 GAE mg/g DW), followed by the Huyou variety (107.33 mg/g DW), while Wanshuwenzhoumigan variety had the lowest (35.91 mg/g DW). Ferulic acid was the most dominant soluble phenolic acid in the selected young citrus, followed by p-coumaric acid and p-hydroxybenzoic acid, whereas nobiletin and tangeretin were the most abundant flavones in the Ponkan, Ougan, and Wanshuwenzhoumigan varieties. Antioxidant capacity that measured by ABTS, FRAP, and DPPH showed similar trends and was positively correlated with the total phenolic and total flavonoid contents (p < 0.05). Considering the high content of phenolics in the young fruits of Ougan and Huyou variety, those two varieties might be potential resources for extracting phytochemicals for health promotion.
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Amiot, Marie-Josèphe, Christian Latgé, Laurence Plumey, and Sylvie Raynal. "Intake Estimation of Phytochemicals in a French Well-Balanced Diet." Nutrients 13, no. 10 (October 16, 2021): 3628. http://dx.doi.org/10.3390/nu13103628.

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Phytochemicals contribute to the health benefits of plant-rich diets, notably through their antioxidant and anti-inflammatory effects. However, recommended daily amounts of the main dietary phytochemicals remain undetermined. We aimed to estimate the amounts of phytochemicals in a well-balanced diet. A modelled diet was created, containing dietary reference intakes for adults in France. Two one-week menus (summer and winter) were devised to reflect typical intakes of plant-based foods. Existing databases were used to estimate daily phytochemical content for seven phytochemical families: phenolic acids, flavonoids (except anthocyanins), anthocyanins, tannins, organosulfur compounds, carotenoids, and caffeine. The summer and winter menus provided 1607 and 1441 mg/day, respectively, of total polyphenols (phenolic acids, flavonoids, anthocyanins, and tannins), the difference being driven by reduced anthocyanin intake in winter. Phenolic acids, flavonoids (including anthocyanins), and tannins accounted for approximately 50%, 25%, and 25% of total polyphenols, respectively. Dietary carotenoid and organosulfur compound content was estimated to be approximately 17 and 70 mg/day, respectively, in both seasons. Finally, both menus provided approximately 110 mg/day of caffeine, exclusively from tea and coffee. Our work supports ongoing efforts to define phytochemical insufficiency states that may occur in individuals with unbalanced diets and related disease risk factors.
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8

Srivastava, Neeharika, Aishwarya Singh Chauhan, and Bechan Sharma. "Isolation and Characterization of Some Phytochemicals from Indian Traditional Plants." Biotechnology Research International 2012 (December 11, 2012): 1–8. http://dx.doi.org/10.1155/2012/549850.

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The present study was designed to evaluate relative contribution of different polyphenols (total phenolics, flavonoids, flavonols) and their antioxidants activities in aqueous extracts of different parts of some plants; Argemone mexicana, Datura metel, Calotropis procera, Thevetia peruviana, and Cannabis sativa. The antioxidants (total phenolics, flavonoids, flavones) were determined by chemical methods. The antioxidant capacities of these extracts were evaluated by FRAP assay. The results demonstrated that phenolic content was maximally present in leaves of T. peruviana. This plant exhibited minimum phenolic content in its flower as compared to other plants. The flower of D. metel contained maximum phenolic content. The flavonoids were present in highest quantity in leaves of C. procera while T. peruviana flowers showed maximum flavonoid content. The fruits of C. sativa contained maximum quantity of flavonoid as compared to other plants tested. The flower extract of C. sativa possessed highest FRAP value followed by A. mexicana and fruit of C. procera. The values of ratios of different polyphenolic compounds present in plant extracts indicated that flower of D. metel contained maximum total flavonoids and minimum phenolics. These results suggested that levels of total phenolics, flavonoids and their FRAP indices exhibited specificity to different plants and their parts.
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9

Prasad, Nagendra, Bao Yang, Kin Weng Kong, Hock Eng Khoo, Jian Sun, Azrina Azlan, Amin Ismail, and Zulfiki Bin Romli. "Phytochemicals and Antioxidant Capacity fromNypa fruticansWurmb. Fruit." Evidence-Based Complementary and Alternative Medicine 2013 (2013): 1–9. http://dx.doi.org/10.1155/2013/154606.

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Nypa fruticansWurmb. is one of the important underutilized fruit of Malaysia, which lacks scientific attention. Total phenolics, flavonoid content, and antioxidant capacities from endosperm extracts ofNypa fruticans(unripe and ripe fruits) were evaluated. Endosperm extract of unripe fruits (EEU) exhibited the highest phenolics (135.6 ± 4.5 mg GAE/g), flavonoid content (68.6 ± 3.1 RE/g), and antioxidant capacity. Free radical scavenging capacity of EEU as assessed by 2-2′-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picryl hydrazyl (DPPH) radicals showed inhibitory activity of78±1.2% and85±2.6%, respectively. Beta carotene bleaching coefficient of EEU was higher (2550±123), when compared to endosperm extract of ripe fruits (1729±172). Additionally, EEU exhibited high antioxidant capacity by phosphomolybdenum method and ferric reducing antioxidant power values. Eight phenolic compounds fromNypa fruticansendosperm extracts were identified and quantified by ultra-high-performance liquid chromatography. Chlorogenic acid, protocatechuic acid, and kaempferol were the major phenolic compounds. Thus this fruit could be used as a potential source of natural antioxidant.
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Gebashe, Fikisiwe, Adeyemi O. Aremu, Jiri Gruz, Jeffrey F. Finnie, and Johannes Van Staden. "Phytochemical Profiles and Antioxidant Activity of Grasses Used in South African Traditional Medicine." Plants 9, no. 3 (March 17, 2020): 371. http://dx.doi.org/10.3390/plants9030371.

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Grasses are a valuable group of monocotyledonous plants, used as nourishing foods and as remedies against diseases for both humans and livestock. Phytochemical profiles of 13 medicinal grasses were quantified, using spectrophotometric methods and ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS), while the antioxidant activity was done using DPPH and ferric-reducing-power assays. The phytochemical analysis included the total soluble phenolic content, flavonoids, proanthocyanidins, iridoids and phenolic acids. Among the 13 grasses, the root methanolic extracts of Cymbopogon spp., Cymbopogon nardus and Cenchrus ciliaris contained the highest concentrations of total soluble phenolics (27–31 mg GAE/g DW) and flavonoids (4–13 mg CE/g DW). Condensed tannins and total iridoid content were highest (2.3 mg CCE/g DW and 3.2 mg HE/g DW, respectively) in Cymbopogon nardus. The most common phenolic compounds in the grass species included ρ-coumaric, ferulic, salicylic and vanillic acids. In the DPPH radical scavenging assay, the EC50 values ranged from 0.02 to 0.11 mg/mL for the different grasses. The best EC50 activity (lowest) was exhibited by Cymbopogon nardus roots (0.02 mg/mL) and inflorescences (0.04 mg/mL), Cymbopogon spp. roots (0.04 mg/mL) and Vetiveria zizanioides leaves (0.06 mg/mL). The highest ferric-reducing power was detected in the whole plant extract of Cynodon dactylon (0.085 ± 0.45; r2 = 0.898). The observed antioxidant activity in the various parts of the grasses may be due to their rich pool of phytochemicals. Thus, some of these grasses provide a source of natural antioxidants and phytochemicals that can be explored for their therapeutic purposes.
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11

Hoang, Hai Ly, and Hafeezur Rehman. "Unravelling the Morphological, Physiological, and Phytochemical Responses in Centella asiatica L. Urban to Incremental Salinity Stress." Life 13, no. 1 (December 25, 2022): 61. http://dx.doi.org/10.3390/life13010061.

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Centella asiatica L. as a traditional medicinal plant is popular in several Asian countries and characterized by the presence of phytochemicals, such as phenolics and flavonoids. Soil salinity can affect the growth and phytochemical composition in this plant species. In this study, the effects of incremental soil salinity (0, 25, 50, 75, and 100 mM NaCl) on growth, physiological characteristics, total phenolic and total flavonoid contents, including the antioxidant activity of Centella asiatica L., were evaluated under greenhouse conditions. Salinity stress reduced growth, biomass production, and total chlorophyll contents, while increasing electrolyte leakage, Na+ and Cl− contents in the shoots and roots. With the increase of salt concentration, total phenolic, total flavonoid content and antioxidant activities were increased. The results showed that centella can tolerate saline conditions up to 100 mM NaCl. Na+ exclusion from the roots, and that increases of phytochemical content in the shoots were related to the salt tolerance of this species.
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12

Makwana, Hiren V., Priyanka G. Pandey, and Binita A. Desai. "Phytochemical Analysis and Evaluation of Total Phenolic Content of Algal Biomass Found in Tapi River in Surat." International Journal for Research in Applied Science and Engineering Technology 10, no. 4 (April 30, 2022): 2783–87. http://dx.doi.org/10.22214/ijraset.2022.41897.

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Abstract: The present study aims to assess the phytochemicals of algal biomass which has seven species identified microscopically. The qualitative phytochemical analysis was done on all the biomass in the Tapi River, Surat in Gujarat. For the qualitative phytochemical analysis total 12 different parameters were analysed and used on algal biomass. Extract prepared in water. Amongst the water extract showed the presence of a maximum number of phytochemical compounds. Next to that, water extract showed alkaloids, glycosides, flavonoids, saponins, terpenoids, phytosterol, coumarins, tannins, diterpenes, and quinones, carbohydrates, The protein present in algal biomass. The evaluation of total phenolic content presence in algal biomass. Keywords: Algal biomass, Phytochemical analysis, Algae, Phytochemicals, Total phenol content, Qualitative analysis of biomass, Tapi, Biomass Extract, Algae extract.
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13

Zhang, Fangyuan, Fengyuan Liu, Arshad Abbasi, Xiaoxiao Chang, and Xinbo Guo. "Effect of Steaming Processing on Phenolic Profiles and Cellular Antioxidant Activities of Castanea mollissima." Molecules 24, no. 4 (February 15, 2019): 703. http://dx.doi.org/10.3390/molecules24040703.

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The intention of this study was to investigate the effect of steaming processing on phenolic profiles and antioxidant activities in chestnuts. Steaming processing at different times and temperatures depicted diverse impacts on free and bound fractions. Though, bound phenolics were stable but long time steaming at higher temperatures tended to improve the levels of phenolics, flavonoids as well as antioxidant activities in chestnut kernels, by up to 60.11% of the original value. Seven phenolic compounds including ferulic acid, chlorogenic acid, gallic acid, vanillic acid, syringate, p-coumaric acid and quercetin were found to change during thermal processes. Significant relationships (p < 0.05) were identified between total phenolics and total antioxidant activities. However, the consistency of chlorogenic acid (p < 0.01) only with cellular antioxidant activity indicated poor bio-accessibility of the phytochemicals in chestnuts. However, this situation could be partly improved by steaming. Steaming could improve the cellular accessibility of free phytochemicals, particularly, increasing the bio-accessibility by 41.96%. This study provided valuable information on dynamic changes of phenolic profiles and antioxidant activity of chestnuts under a steaming process, which could offer possible guidance for the chestnut processing industry in the future.
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El-Ghorab, Ahmed H., Fathy A. Behery, Mohamed A. Abdelgawad, Ibrahim Hotan Alsohaimi, Arafa Musa, Ehab M. Mostafa, Hamud A. Altaleb, et al. "LC/MS Profiling and Gold Nanoparticle Formulation of Major Metabolites from Origanum majorana as Antibacterial and Antioxidant Potentialities." Plants 11, no. 14 (July 18, 2022): 1871. http://dx.doi.org/10.3390/plants11141871.

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Origanum majoranum L. is a Lamiaceae medicinal plant with culinary and ethnomedical applications. Its biological and phytochemical profiles have been extensively researched. Accordingly, this study aimed to investigate the chemical composition and the antibacterial and antioxidant properties of O. majoranum high features, as well as to search for techniques for activity optimization. A metabolomics study of the crude extract of O. majoranum using liquid chromatography-high-resolution electrospray ionization mass spectrometry (LC ± HR ± ESI ± MS) was conducted. Five fractions (petroleum ether, dichloromethane, ethyl acetate, n-butanol, and aqueous) were derived from the total extract of the aerial parts. Different chromatographic methods and NMR analysis were utilized to purify and identify the isolated phenolics (high features). Moreover, the antimicrobial, antibiofilm, and antioxidant activity of phenolics were performed. Results showed that metabolomic profiling of the crude extract of O. majoranum aerial parts revealed the presence of a variety of phytochemicals, predominantly phenolics, resulting in the isolation and identification of seven high-feature compounds comprising two phenolic acids, rosmarinic and caffeic acids, one phenolic diterpene, 7-methoxyepirosmanol, in addition to four flavonoids, quercetin, hesperitin, hesperidin, and luteolin. On the other hand, 7-methoxyepirosmanol (OM1) displayed the most antimicrobial and antioxidant potential. Such a phenolic principal activity improvement seems to be established after loading on gold nanoparticles.
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15

Polyium, Udomdeja. "Phytochemicals Investigation and Antioxidant Activities of the Xylocarpus granatum Extracts." Applied Mechanics and Materials 901 (August 2020): 17–21. http://dx.doi.org/10.4028/www.scientific.net/amm.901.17.

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This study aimed to investigate the phytochemicals and antioxidant activities of the Xylocarpus granatum Koen fruits extract. The phytochemical analysis was performed for alkaloid, tannin, terpenoid, flavonoid, anthraquinone, and cardiac glycoside. The fruits of X. granatum was separated by maceration and sequential extraction method with hexane, ethyl acetate, and methanol to crude extracts. Crude extracts were assessed for their total phenolic contents tested using the Folin–Ciocalteu method and DPPH radical scavenging assay using DPPH radical scavenging assay. The results showed that phytochemical screening of X. granatum fruits have the presence of alkaloid, tannin, terpenoid, flavonoid, anthraquinone, and cardiac glycoside. The crude methanol extract had the highest phenolic content and reduced DPPH; it supports traditional uses in folk medicine.
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16

Kerdchan, K., and P. Srihanam. "Fractionation of Sugarcane Extracts and Determination of Phenolic Compounds and their Antioxidant Activity." Asian Journal of Chemistry 34, no. 2 (2022): 284–88. http://dx.doi.org/10.14233/ajchem.2022.23474.

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Crude ethanolic extracts of four cultivars of sugarcane (Saccharum officinarum L.) were firstly prepared and then fractionated using silica gel column chromatography before the determination of their oxidative substances. The fractionated extracts were then tested for antioxidant activity by various assays. Finally, high-performance liquid chromatography (HPLC) was applied for the quantitative determination of the individual phenolic compounds. Sub-fraction 2 has the highest phytochemical contents as well as antioxidant activity. All tested phytochemicals had positively correlated to antioxidant activity. HPLC analysis showed that the phytochemicals in the fractionated extracts varied by the sugarcane cultivars. The main flavonoid substances found in the fractionated extracts were epicatechin, catechin, quercetin, resveratrol, myricetin and rutin while gallic acid was the main phenolic substance. The obtained information is useful for further studies and applications.
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17

KING, AMY, and GLORIA YOUNG. "Characteristics and Occurrence of Phenolic Phytochemicals." Journal of the American Dietetic Association 99, no. 2 (February 1999): 213–18. http://dx.doi.org/10.1016/s0002-8223(99)00051-6.

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Ahmed, Fouad A., and Rehab F. M. Ali. "Bioactive Compounds and Antioxidant Activity of Fresh and Processed White Cauliflower." BioMed Research International 2013 (2013): 1–9. http://dx.doi.org/10.1155/2013/367819.

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Brassicaspecies are very rich in health-promoting phytochemicals, including phenolic compounds, vitamin C, and minerals. The objective of this study was to investigate the effect of different blanching (i.e., water and steam) and cooking (i.e., water boiling, steam boiling, microwaving, and stir-frying) methods on the nutrient components, phytochemical contents (i.e., polyphenols, carotenoids, flavonoid, and ascorbic acid), antioxidant activity measured by DPPH assay, and phenolic profiles of white cauliflower. Results showed that water boiling and water blanching processes had a great effect on the nutrient components and caused significant losses of dry matter, protein, and mineral and phytochemical contents. However, steam treatments (blanching and cooking), stir-frying, and microwaving presented the lowest reductions. Methanolic extract of fresh cauliflower had significantly the highest antioxidant activity (68.91%) followed by the extracts of steam-blanched, steam-boiled, stir-fried, and microwaved cauliflower 61.83%, 59.15%, 58.93%, and 58.24%, respectively. HPLC analysis revealed that the predominant phenolics of raw cauliflower were protocatechuic acid (192.45), quercetin (202.4), pyrogallol (18.9), vanillic acid (11.90), coumaric acid (6.94), and kaempferol (25.91) mg/100 g DW, respectively.
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Duangpapeng, Prakasit, Danupol Ketthaisong, Khomsorn Lomthaisong, Kamol Lertrat, Marvin Scott, and Bhalang Suriharn. "Corn Tassel: A New Source of Phytochemicals and Antioxidant Potential for Value-Added Product Development in the Agro-Industry." Agronomy 8, no. 11 (October 31, 2018): 242. http://dx.doi.org/10.3390/agronomy8110242.

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Corn tassel is a byproduct from hybrid corn seed production. It is also a new source of phytochemicals, including compounds with antioxidant activity. The aim of this study was to evaluate the phytochemical content and antioxidant potential of different corn tassel development stages. A 4 × 8 factorial experiment consisting of four tassel development stages and eight commercial corn varieties was carried out with a randomized complete block design with three replications. Data were collected for tassel weight, concentration and yield of total phenolics, total anthocyanin concentration, total carotenoid concentration, and antioxidant activity, as determined by the 2,2-diphenyl-1-picrylhydrazyl(DPPH•) radical scavenging assay and Trolox equivalent antioxidant capacity(TEAC) assays. Corn varieties and tassel developmental stages showed significant variations (p ≤ 0.01) in all parameters. P4546 presented the best variety for the total phenolic content (15.8 mg GAE/g DW sample), whereas Hibrix3 had the highest phenolic yield (6.78 kg GAE/ha). KGW1, a purple waxy variety, had the highest anthocyanin content (1528.0 µg CGE/g DW sample), anthocyanin yield (753.0 g CGE/ha), carotenoid content (74.9 µg/g DW sample), and carotenoid yield (53.7 g/ha). P4546 had the highest antioxidant activity: 92.4% for DPPH• and 76.1 µmol TE/g DW sample for the TEAC assay, respectively. The most appropriate time for tassel harvest to ensure the production of phytochemicals with high antioxidant activity should be from the 1st day of pollen shed until 50% of pollen shed. Phytochemicals and antioxidants that are extracted from corn tassel can be used as a functional food supplement, as natural pharmaceuticals, and in cosmetic products.
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Srihanam, Prasong, and Patthraporn Siripipatthana. "Phytochemical Content and Antioxidant Activity in Wild Grape (Ampelocissus martini Planch.) Root Extracts." Oriental Journal of Chemistry 35, no. 6 (November 22, 2019): 1690–94. http://dx.doi.org/10.13005/ojc/350609.

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The factionated extracts of wild grape (Ampelocissus martini Planch.) roots by silica gel chromatography eluting with different solvent systems were firstly prepared, and then were assayed for their phytochemical contents and antioxidant activity. A high level of total phenolic and flavonoid content were found in both crude extract and chromatographic fractions. The ethyl acetate: methanol (75:25 %v/v) fraction had the highest of total phenolic and flavonoid as well as antioxidant activity assessed all methods, except ABTS assay. This indicated that eluting solvents directly affected phytochemical profile and activity. Moreover, the phenolic and flanonoid substances showed highly possitive correlation coefficient (r) to antioxidant activity. This suggested that the wild grape root is a natural source containing high phytochemicals with antioxidant activity which might be used as active ingredient for health supporting.
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Generalić Mekinić, Ivana, Danijela Skroza, Vida Šimat, Imen Hamed, Martina Čagalj, and Zvjezdana Popović Perković. "Phenolic Content of Brown Algae (Pheophyceae) Species: Extraction, Identification, and Quantification." Biomolecules 9, no. 6 (June 22, 2019): 244. http://dx.doi.org/10.3390/biom9060244.

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Over the last few decades, isolations and chemical characterizations of secondary metabolites with proved biological activities have been of interest for numerous research groups across the world. Phenolics, as one of the largest and most widely distributed group of phytochemicals, have gained special attention due to their pharmacological activity and array of health-promoting benefits. Reports on phenolic potentials of marine algae, especially brown algae (Pheophyceae) that are characterized by the presence of phlorotannins, are still scarce. The aim of this review paper is to provide an overview of current knowledge about phenolic potential of different brown algae species (74 species from 7 different orders). Studies on brown algae phenolics usually involve few species, thus the focus of this review is to provide information about the phenolic potential of reported algae species and to get an insight into some issues related to the applied extraction procedures and determination/quantification methods to facilitate the comparison of results from different studies. The information provided through this review should be useful for the design and interpretation of studies investigating the brown algae as a source of valuable phytochemicals.
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Mohammed, Messaoudi, and Merah Maroua. "Phytochemicals Levels and Antioxidant Capacities of Figs Flowers Fruits." Nepal Journal of Biotechnology 8, no. 1 (July 31, 2020): 29–35. http://dx.doi.org/10.3126/njb.v8i1.30207.

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Since antiquity, phenolic compounds produced by plants were known as free radical scavengers and as powerful antioxidants. Huge interest has been made by researchers to the traditional uses of medicinal plants against illnesses related to oxidative stress. This study measures the correlation that can be existed between the antioxidant capacity and phytochemical s levels of four varieties of Ficus carica fruits, figs flowers or "Bakor" as called locally in Algeria. Therefore, extracts were assessed for determining their antioxidative potentials using both test of total antioxidant capacity and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging test followed by quantitative phytochemical analysis to estimate the total flavonoid level (TFL), the total phenolic level (TPL), the total anthocyanin level (TAL) and the condensed tannins level of plants methanolic extracts. A positive correlation was observed between phenolics content and the antioxidant capacity of figs flowers methanol extracts. The methanolic extract of Bechar (MeOH Var.2) chelated 87,9± 1,23 % of the DPPH free radical with IC50 value equal to 0,185 mg/g DW. A high antioxidant ability of almost all extracts is, probably, related to the appreciable rates of flavonoids, phenolics and tannins showed by those fig extracts. The highest value of phenolics level was detected among the variety 1 methanolic extract of Bechar (MeOH Var.1) of 10,4 mg GAE/g DW.
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Borges, Josileide Gonçalves, Jackson Roberto Guedes da Silva Almeida, Juliana Anielle Ribeiro De Sá, Kaline Stela Pires Bezerra, Patricia Kauanna Fonseca Damasceno, Isabela Mary Alves Reis, and Marigilson Pontes De Siqueira Moura. "Inhibition of Pancreatic Lipase and Characterization by Extracts From Fruit Pomaces Cultivars Grown in Brazilian Semiarid." Journal of Agricultural Studies 7, no. 2 (November 10, 2019): 343. http://dx.doi.org/10.5296/jas.v7i4.15410.

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Investigate action of pancreatic lipase inhibition and characterization of extracts obtained from fruit pomace cultivars grown in Brazilian semiarid. Phytochemicals of Vitis labrusca, Passiflora edulis flavicarpa Deg., Citrus sinensis L Osbeck, and Punica granatum L. were extracted by maceration in a hydroethanolic solution. Inhibition activity of pancreatic lipase was performed by ELISA reader. To identify which compounds would probably be responsible for inhibitory activity, extracts were characterized by method of total phenolics using folin ciocalteau, phenolic acids and flavonoids were identified by HPLC, antioxidant potential was quantified by DPPH. With exception of pomegranate seed, all other pomace showed potential for inhibition of lipase. However, V. labrusca had higher percentage of inhibition (89.74%), this percentage similar to that obtained by commercial orlistat inhibitor, which would indicate its use as an efficient inhibitor. For all extracts were found several phytochemical compounds with phenolic characteristics, however, pomaces of P. granatum peel and V. labrusca showed best results in total phenolic and antioxidant activity. P. granatum peel had a high content of total phenolics (82.50 EAG g-1) and the best antioxidant activity (IC50 = 1.47 µg mL-1). All pomace extracts showed varied secondary metabolites, although antioxidant activity from grape and passion fruit pomaces showed potential for inhibition of lipids absorption.
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24

Ghimire, Bimal Kumar, Ji-Won Seo, Seung-Hyun Kim, Balkrishna Ghimire, Jae-Geun Lee, Chang-Yeon Yu, and Ill-Min Chung. "Influence of Harvesting Time on Phenolic and Mineral Profiles and Their Association with the Antioxidant and Cytotoxic Effects of Atractylodes japonica Koidz." Agronomy 11, no. 7 (June 29, 2021): 1327. http://dx.doi.org/10.3390/agronomy11071327.

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Plant phytochemical accumulation is influenced by various external factors that change with the seasons (e.g., harvesting time). Atractylodes japonica, an important medicinal plant rich in bioactive compounds, is used to treat several human diseases. We analyzed the influence of harvesting time on phenolic compound concentration and antioxidant activity of A. japonica roots. We investigated the correlation between phenolic compound and minerals contents and antioxidant activity in different harvests. Total phenolic and flavonoid contents varied significantly with the harvesting time. Liquid chromatography–mass spectrometry/mass spectrometry (LC–MS/MS) analysis revealed significant changes in the concentration of various phenolics between harvests. The content of different types of phenolics were significantly higher in the samples collected in October. Among them, chlorogenic acids (133,833.30 µg/g dry weight of root extract) were the most dominant phytochemical compounds detected. Samples harvested in October had higher concentrations of flavonoids, including rutin, orientin, vitexin, and apigenin. Roots harvested in October had a significantly higher (p < 0.05) antioxidant activity than that of those harvested later. Root mineral concentrations also varied with the harvest time. The analysis revealed that macro elements such as Ca ad Mg contents were significantly increased with delaying harvesting time, whereas a different trend was observed for the microelements including Fe, Cu, Al, and As contents in the October harvest. We also found a significant relationship between antioxidant activity and phenolic compound content. The most abundant minerals (Ca, Mg, Mn, Fe, and Al) correlated positively with the antioxidant activity indicating that these elements and compounds may be associated with the A. japonica antioxidant potential. Furthermore, A. japonica root extracts inhibited NIH/3T3 cellular proliferation in a season- and dose-dependent manner. Hence, harvesting time influenced the antioxidant properties and phenolic compound accumulation of A. japonica roots. These results indicate that the harvesting time is essential for obtaining the specific phytochemicals.
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25

Mahto, Harishankar, Dipa Mahato, and Hanuman Prasad Sharma. "Phytoconstituent estimation and LC-MS studies of field grown Withania somnifera (L.) Dunal root extract in Jharkhand and Bihar." Research Journal of Chemistry and Environment 26, no. 11 (October 25, 2022): 135–41. http://dx.doi.org/10.25303/2611rjce1350141.

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Plants in either raw form or their root extracts are utilized as complementary and alternative medicine in various disorders. The present study was undertaken for phytoconstituents estimation and to evaluate chemical compositions of methanolic extract of Withania somnifera (L.) Dunal (Ashwagandha) roots collected from BAU, Ranchi, Jharkhand and Sabour, Bhagalpur, Bihar. Phytochemicals through crude methanolic extract were analyzed in Ashwagandha roots. Alcoholic extract was prepared using Soxhlet extraction apparatus. On phytochemical evaluation, total phenolic, flavonoid, total tannin and alkaloids were estimated. Presence of bioactive components in extract was determined by Liquid Chromatography-Mass Spectrophotometry (ESI positive ion) technique. Qualitative screening of extract ensured the presence of alkaloids, phenols, tannins and flavonoids. Quantitatively these phytochemicals showed TPC (66.06 mg/gm), TFC (650.0 mg/gm), TAC (102 mg/gm) and TTC (5.89 mg/gm). However, the phytochemicals and yield did not accumulate in various fractions on polarity. This result was supported by LC-MS analysis which showed 17 definite peaks in their chromatogram but only 8 peaks offer a suitable approximation. Extract revealed forty five bioactive compounds where most of them were phenolic compounds, aryl propionic acid and cinnamide derivatives. Quantitative analysis of present phytochemical showed maximum quantity of TFC followed by TAC, TPC and TTC. On the other hand, presence of various bioactive compounds in root extract showed that plant is having very high medicinal value.
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26

Guo, Huina, Arpamas Chariyakornkul, Warunyoo Phannasorn, Sugunya Mahatheeranont, and Rawiwan Wongpoomchai. "Phytochemical Profile and Chemopreventive Properties of Cooked Glutinous Purple Rice Extracts Using Cell-Based Assays and Rat Model." Foods 11, no. 15 (August 4, 2022): 2333. http://dx.doi.org/10.3390/foods11152333.

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Purple rice has gained attention for its health promoting potential due to a high content of bioactive phytochemicals. The heat generated during cooking alters the quality and quantity of nutrients and phytochemicals in food. This study aimed to investigate the phytochemical profile and chemopreventive properties of cooked glutinous purple rice using cell-based assays and a rat model. Purple rice was cooked in a rice cooker and was then further extracted with solvents to obtain dichloromethane and methanol extracts. The methanol extracts of glutinous purple rice contained great amounts of phenolics, flavonoids, and anthocyanins. Protocatechuic acid (2.26–5.40 mg/g extract) and cyanidin 3-glucoside (34.3–65.7 mg/g extract) were the major phenolic acid and anthocyanin contents, respectively. After cooking, the content of anthocyanins, γ-oryzanols, and phytosterols decreased, while the amount of some phenolic acid and tocol contents increased. Methanol extracts of glutinous purple rice inhibited reactive oxygen species production about 60% in PMA-treated peripheral blood mononuclear cells, reduced nitric oxide formation in LPS-induced RAW 264.7 cells (26–39% inhibition), and exhibited antimutagenicity against several mutagens using the Ames test, but dichloromethane extracts presented only mild anti-inflammatory activities. Although methanol extracts induced mild mutagenicity (mutagenic index 2.0–2.5), they did not induce micronucleated hepatocyte formation and certain hepatic CYP450 isozyme activities in rats. However, the mutagenicity of the methanol extract significantly declined after cooking. In summary, the methanol extract of the cooked glutinous purple rice might be a promising cancer chemopreventive fraction, which was neither genotoxic nor posing adverse effects on phytochemical–drug interaction in rats.
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27

Kandylis, Panagiotis. "Phytochemicals and Antioxidant Properties of Edible Flowers." Applied Sciences 12, no. 19 (October 2, 2022): 9937. http://dx.doi.org/10.3390/app12199937.

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Nowadays there is a growing interest among consumers for functional food products, and edible flowers could be a solution to fulfill this demand. Edible flowers have been used throughout the centuries for their pharmaceutical properties, but also in some areas for culinary purposes. There is a great variety of edible flowers, and numerous studies are available regarding their chemical composition and potential antioxidant and functional characteristics. Therefore, the present work focuses on gathering a vast amount of data regarding edible flowers. Phytochemical content, total phenolic content, total flavonoid content and antioxidant activity (DPPH, FRAP, ABTS, etc.) of more than 200 edible flowers are presented. The main phytochemicals belong to the groups of phenolic acids, flavonoids, carotenoids and tocols, while great variability is reported in their content. The present study could be a useful tool to select the edible flowers that can be served as sources of specific phytochemicals with increased antioxidant activity and evaluate them for their safety and potential application in food industry, during processing and storage.
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28

Nyau, V., S. Prakash, J. Rodrigues, and J. Farrant. "Domestic Cooking Effects of Bambara Groundnuts and Common Beans in the Antioxidant Properties and Polyphenol Profiles." Journal of Food Research 6, no. 2 (February 7, 2017): 24. http://dx.doi.org/10.5539/jfr.v6n2p24.

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Processing of legumes before consumption has several effects on micronutrients, macronutrients and phytonutrients. This study was undertaken to investigate the effect of domestic processing on antioxidant activities and phenolic phytochemicals of the red bambara groundnuts and red beans. The study employed in vitro antioxidant assays (DPPH and FRAP) to screen for antioxidant properties, HPLC-PDA-ESI-MS and Folin Ciocalteu assay to screen for phenolic phytochemical profiles. Domestic cooking displayed positive effects on the antioxidant activity and phenolic phytochemical profiles of the two legumes. The free radical scavenging speed increased 10-fold in the methanolic extract from cooked red bambara groundnuts compared to uncooked. By contrast, the free radical scavenging speed increased 20-fold in the methanolic extract from cooked red beans compared to uncooked. HPLC-PDA-ESI-MS profiles of the cooked red bambara groundnuts and red beans revealed a number of emergent phenolic compounds, mainly flavonoids. These data indicate that cooking appear to enhance the nutraceutical profiles of the legumes investigated.
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29

Saakian, Alexander. "Effect of mineral fertilizers on the variability of phytochemicals in kale." АгроЭкоИнфо 1, no. 43 (February 28, 2021): 8. http://dx.doi.org/10.51419/202111080.

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The effect of five mineral nutrients on the variability of the content of biochemical compounds, such as flavonoids, phenolic compounds, vitamin C, carotenoids and the antioxidant activity of kale (Brassica Oleracea var. sabellica) collected at the edible maturity stage. Manipulation of the phytochemical composition of the kale cabbage using mineral fertilizers was evident in the results achieved. The various mineral nutrients used had different effects on the content of the studied phytochemicals. The content of flavonoids and carotenoids did not decrease with any of the various mineral nutrients used. Contrary to recent research suggesting that rare earth elements are good stimulators of secondary metabolite accumulation, our results showed a decrease in the content of phenolic compounds in plants fertilized with neodymium. The total antioxidant activity increased under the influence of NPK and humate, which correlated with the highest content of soluble phenolic compounds and flavonoids in kale leaves. Low doses of N have a positive effect on these indicators. Mineral supplements of trace elements also had a stimulating effect on the accumulation of the studied substances. However, in addition to trace elements, a significant role is played by the presence of humic acids (in humate), which together with trace elements have the maximum stimulating effect on the content of the substances under study. Keywords: MINERAL FERTILIZERS, ANTIOXIDANT PHYTOCHEMICALS, PHENOLIC COMPOUNDS, CAROTENOID, ANTIOXIDATIVE ACTIVITY, VITAMIN C, FLAVONOIDS, BRASSICA VEGETABLES
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30

Saakian, Alexander. "Effect of mineral fertilizers on the variability of phytochemicals in kale." АгроЭкоИнфо 1, no. 43 (February 28, 2021): 8. http://dx.doi.org/10.51419/20211108.

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The effect of five mineral nutrients on the variability of the content of biochemical compounds, such as flavonoids, phenolic compounds, vitamin C, carotenoids and the antioxidant activity of kale (Brassica Oleracea var. sabellica) collected at the edible maturity stage. Manipulation of the phytochemical composition of the kale cabbage using mineral fertilizers was evident in the results achieved. The various mineral nutrients used had different effects on the content of the studied phytochemicals. The content of flavonoids and carotenoids did not decrease with any of the various mineral nutrients used. Contrary to recent research suggesting that rare earth elements are good stimulators of secondary metabolite accumulation, our results showed a decrease in the content of phenolic compounds in plants fertilized with neodymium. The total antioxidant activity increased under the influence of NPK and humate, which correlated with the highest content of soluble phenolic compounds and flavonoids in kale leaves. Low doses of N have a positive effect on these indicators. Mineral supplements of trace elements also had a stimulating effect on the accumulation of the studied substances. However, in addition to trace elements, a significant role is played by the presence of humic acids (in humate), which together with trace elements have the maximum stimulating effect on the content of the substances under study. Keywords: MINERAL FERTILIZERS, ANTIOXIDANT PHYTOCHEMICALS, PHENOLIC COMPOUNDS, CAROTENOID, ANTIOXIDATIVE ACTIVITY, VITAMIN C, FLAVONOIDS, BRASSICA VEGETABLES
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31

Osman Adam, Osman Adam, Ragaa Satti Mohmmed Abadi, and Saad Mohamed Hussein Ayoub. "Antioxidant activity, Total Phenolic and Flavonoid Contents and Cytotoxic activity of Euphorbia aegyptiaca." Journal of Drug Delivery and Therapeutics 10, no. 2 (March 15, 2020): 37–41. http://dx.doi.org/10.22270/jddt.v10i2.3911.

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Euphorbia aegyptiaca is a herbaceous plant traditionally used in Sudan for treatment of various diseases, and the study of this plant is still limited. The aim of the present study was to screen the phytochemicals and to assess the Antioxidant activity, total phenolic, flavonoid contents and cytotoxic activity of Euphorbia aegyptiaca. The plant material was extracted successively by Soxhlet apparatus using n-hexane, chloroform and methanol. The chemical constituents of the extracts were carried out using the standard procedures. The Folin- Ciocalteu and Aluminium chloride method was employed to calculate the total phenolic and flavonoid content, respectively. The antioxidant activity, was assessed by measuring the scavenging activity of the DPPH (2.2Di (4-tert-octylphenyl)-1-picryl-hydrazyl) and Propyl Gallate as standard antioxidants. While cytotoxic activities were screened using brine shrimp. Phytochemical screening studies revealed that flavonoids, tannins, coumarins, saponins, sterols, terpenes, anthraquinones and alkaloids were the main phytochemicals present in extracts of E. aegyptiaca. The methanol extract showed the highest level of total phenolic contents (173.49±2.427 mg GAE/g) and flavonoid content (239.53±7.90 mg QE/g), and the highest antioxidant activity 89% with least (IC50 0.0449µg/ml), and the no toxicity against brine shrimp (LD50 3423.156). Furthermore, no toxicity in all extracts was observed. The present study is the first evaluation regarding the characterization of E. aegyptiaca and its safety, and the results demonstrate its antioxidant potential and suggest its safe therapeutic use. The results suggest that methanol extract is a rich source of phytochemicals and exhibits highest amount of and total phenolic, flavonoid content and significant antioxidant activity and it has no cytotoxic activity. E. aegyptiaca plant can be regarded as a promising Source of naturally occurring potential antioxidants. Keywords: Euphorbia aegyptiaca, Sudan, Antioxidant, Total phenolic, total flavonoid, Cytotoxicity.
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Moongngarm, Anuchita, Amaraphon Homduang, and Wariya Hochin. "Changes of Phytochemical Contents in Sweet and Waxy Corn (Zea mays L.) as Affected by Cultivars and Growth Stages." Current Nutrition & Food Science 16, no. 2 (February 14, 2020): 162–69. http://dx.doi.org/10.2174/1573401315666181218125559.

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Background: Sweet and waxy corn (Zea mays L.) are high in phytochemicals which vary depending on several factors including corn cultivars and the maturation stage. Methods: Five commercial sweet and waxy corn cultivars were grown and their major phytochemical compositions were investigated at the milk, late milk, and soft dough stages. Phytochemicals in corn samples harvested from each kernel maturation stage were determined as total phenolic compounds, anthocyanins, tocopherols, and carotenoids. Results: Significant interactions between the corn cultivars and maturation stage were detected for all phytochemical compositions, with changes in their concentration during seed development. Total phenolic compounds and total anthocyanins were highest in the dark purple waxy variety (316.74 mg GAE/100 g of DW and 522.21 mg CGE/100 g of DW, respectively) in which cyanidin was the major anthocyanin. Carotenoid compositions were also affected by corn type and harvesting stage. The yellow sweet cultivars indicated higher levels of carotenoids than other colors, with lutein as the major carotenoid at concentrations from 0.05 to 8.46 µg/g of DW. Contents of γ-tocotrienol, γ-tocopherol, and α-tocopherol were observed in all five corn cultivars with similar influences of the maturation stage. Conclusion: Results suggested that the phytochemical contents of all five corn types showed diverse reliance regarding cultivars and maturity stages. The purple waxy and yellow sweet corn cultivars contained a high level of phytochemicals. This would open up a useful opportunity for the functional food industry as consumption of these corn cultivars would afford health benefits to consumers.
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33

Pungot, Noor Hidayah. "Potential of Malaysian Cherry Leaves (Muntingia calabura) as an Antioxidant Agent." Science Letters 14, no. 2 (June 1, 2020): 106. http://dx.doi.org/10.24191/sl.v14i2.9547.

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Muntingia calabura has a high phytochemical content, especially the phenolic group that can act as antioxidant. In Malaysia country, this M. calabura also known as ‘kerukup siam’ or ‘Ceri Kampung’ and it belongs to Muntingiaceae family. This research was conducted to determine the potential of antioxidant activity application of cherry leaves (M. calabura) from various solvent extracts (methanol, ethyl acetate, and n-hexane). The phytochemical contents were screening by using the established standard procedure. Total phenolic content (TPC) was determined according to the Folin-Ciocalteau colorimetric method, while the antioxidant activity was carried out using 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging assay. Phytochemical screening on the leaves part methanolic extracts revealed that the presence of various biochemicals like flavonoids, phenols, steroids, triterpenes, tannins, reducing sugars, and saponins except the alkaloids. Among the three extracts, the methanol leaf extract gave the highest content of phenolics (8.20 mg GAE/g extract). Analyses of antioxidant activity with DPPH method showed that cherry leaf methanolic extracts produced high antioxidant activity with IC50 value of 167.70 mg/mL. The present study confirms that the presence of various phytochemicals which shows good antioxidant activity of M. calabura leaves. Therefore, it has the potential as a therapeutic antioxidant agent and can be used in cosmeceutical and food products.
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Cañas, Silvia, Miguel Rebollo-Hernanz, Cheyenne Braojos, Vanesa Benítez, Rebeca Ferreras-Charro, Montserrat Dueñas, Yolanda Aguilera, and María A. Martín-Cabrejas. "Understanding the Gastrointestinal Behavior of the Coffee Pulp Phenolic Compounds under Simulated Conditions." Antioxidants 11, no. 9 (September 15, 2022): 1818. http://dx.doi.org/10.3390/antiox11091818.

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Numerous residues, such as the coffee pulp, are generated throughout coffee processing. This by-product is a source of antioxidant phytochemicals, including phenolic compounds and caffeine. However, the antioxidant properties of the phenolic compounds from the coffee pulp are physiologically limited to their bioaccessibility, bioavailability, and biotransformation occurring during gastrointestinal digestion. Hence, this study explored the phenolic and caffeine profile in the coffee pulp flour (CPF) and extract (CPE), their intestinal bioaccessibility through in vitro digestion, and their potential bioavailability and colonic metabolism using in silico models. The CPE exhibited a higher concentration of phenolic compounds than the CPF, mainly phenolic acids (protocatechuic, chlorogenic, and gallic acids), followed by flavonoids, particularly quercetin derivatives. Caffeine was found in higher concentrations than phenolic compounds. The antioxidant capacity was increased throughout the digestive process. The coffee pulp matrix influenced phytochemicals’ behavior during gastrointestinal digestion. Whereas individual phenolic compounds generally decreased during digestion, caffeine remained stable. Then, phenolic acids and caffeine were highly bioaccessible, while flavonoids were mainly degraded. As a result, caffeine and protocatechuic acid were the main compounds absorbed in the intestine after digestion. Non-absorbed phenolic compounds might undergo colonic biotransformation yielding small and potentially more adsorbable phenolic metabolites. These results contribute to establishing the coffee pulp as an antioxidant food ingredient since it contains bioaccessible and potentially bioavailable phytochemicals with potential health-promoting properties.
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35

Lima, Nerilson M., Thais O. Falcoski, Romena S. Silveira, Haustin S. Vieira, Vanessa N. C. Santos, Rhianne R. Ramos, Julio Cesar de P. Silva, et al. "Effectiveness of different methods for the extraction of principle actives and phytochemicals content in medicinal herbals." Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas 20, no. 3 (May 30, 2021): 324–38. http://dx.doi.org/10.37360/blacpma.21.20.3.24.

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In this present study, we investigated the influence of various extraction methods including maceration, sonication, infusion, decoction, and microwave extraction, on the chemical and biological potential of phytochemicals extracted from three medicinal plants (Ageratum conyzoides, Plantago major and Arctium lappa L). The results were subsequently analyzed by variance analysis. Our results suggested that sonication is the most effective extraction method among the five methods tested herein, for the extraction of phytochemicals that have a high antioxidant potential and high phenolic content. The three plants employed for this study had a high concentration of flavonoids and phenolics which was compatible with the chemosystematics of the species. All the samples possessed a Sun Protection Factor (SPF) of less than 6. Interestingly, a maximum reaction time of approximately 20 min was noted for the complexation of AlCl3with the flavonoids present in the phytochemical extract during analyses of the kinetic parameters. We finally identified that the Ageratum conyzoides extract, prepared by sonication, possessed a significant pharmacological potential against hepatocarcinoma tumour cells, whose result can guide further studies for its therapeutic efficacy.
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Waterland, Nicole L., Youyoun Moon, Janet C. Tou, Dean A. Kopsell, Moo Jung Kim, and Suejin Park. "Differences in Leaf Color and Stage of Development at Harvest Influenced Phytochemical Content in Three Cultivars of Kale (Brassica oleracea L. and B. napus)." Journal of Agricultural Science 11, no. 3 (February 15, 2019): 14. http://dx.doi.org/10.5539/jas.v11n3p14.

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Microgreens and red colored plants have been suggested to contain higher level of health promoting phytochemicals. Kale (Brassica oleracea L. and other species) is regarded as a &lsquo;superfood&rsquo; due to its antioxidant properties. In our study, three kale cultivars (B. oleracea L. &lsquo;Dwarf Blue Curled&rsquo; and &lsquo;Scarlet&rsquo;, and B. napus &lsquo;Red Russian&rsquo;) differing in leaf pigmentation were harvested at five different leaf developmental stages and the concentrations of bioactive antioxidants compounds were evaluated. Carotenoids were measured by high performance liquid chromatography, and spectrometry analyses were used for total phenolics and anthocyanin measurements. Red leaf kale (&lsquo;Scarlet&rsquo;) was generally higher in total carotenoids, phenolics, and anthocyanins than green leaf kales (&lsquo;Dwarf Blue Curled&rsquo; and &lsquo;Red Russian&rsquo;). As kale matured, water content decreased and dry mass increased. On a dry weight basis, total carotenoids were more abundant in microgreens and young seedlings with 4 to 6 true leaves (baby greens) than adult stage. In contrast, on a fresh weight basis, baby greens or adult kales generally contained more carotenoids and total phenolic compounds than microgreens, more likely due to the lower dry mass and phytochemical accumulation at microgreen stages. Although some microgreens vegetables may contain more health promoting phytochemicals, based on our study, higher phytochemicals were detected in young seedlings or mature leaves of kale.
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37

Abdullah, Fuad O., Faiq H. S. Hussain, Lucia Cucca, and Giovanni Vidari. "Phytochemical Investigation and Antioxidant Effects of Different Solvent Extracts of Pterocephalus Nestorianus Nab. Growing in Kurdistan Region-Iraq." Science Journal of University of Zakho 6, no. 1 (March 30, 2018): 21. http://dx.doi.org/10.25271/2018.6.1.439.

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The medicinal plants have been used as a herbal tradition in Kurdistan. Most of the medicinal plants are still not investigated from a phytochemical and biological activity. Therefore, different solvent extraction carried out to study phytochemicals and antioxidant activity of Pterocephalus nestorianus Nab. Through this research, it is reported for the first time that the phytochemicals and antioxidant activity on P. nestorianus Nab. collected at Safeen Mountain in Erbil-Iraq, which was known as a Lawa in Kurdistan. It is used against inflammation and oral diseases. The phytochemical study of four extracts (Hexane, ethyl acetate, methanol and methanol 70%) of P. nestorianus flowers, leaves and roots revealed that it contains rich phytochemical composition. C4H8O2 and CH3OH extracts of P. nestorianus showed a high radical scavenging activity compared with trihydroxy benzoic acid as a standard. The flavonoid and phenolic presence showed strong correlation with the antioxidant activity.
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38

Pan, Zhibin, Weiqing Cheng, Zhibin Liu, Weibin Wu, Bin Yang, and Junhan Lin. "Comparative Study of the Phytochemical Profiles of the Rhizomes of Cultivated and Wild-Grown Polygonatum sibiricum." Separations 9, no. 12 (November 30, 2022): 398. http://dx.doi.org/10.3390/separations9120398.

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The rhizome of Polygonatum sibiricum is a traditional Chinese medicine material and also a popular functional food consumed in China. Due to the increasing demand and overexploitation, the use of the cultivated plant is growing rapidly. However, the difference in phytochemical profile and health benefit between the cultivated and wild-grown P. sibiricum has not been revealed yet. The objectives of this study are to compare the phytochemical profiles of two types of P. sibiricum, i.e., the cultivated and the wild-grown types, by using UHPLC-Q-Orbitrap-MS based untargeted metabolomics approach. We tentatively identified 190 phytochemicals belonging to alkaloids, flavonoids, phenolic acids, and terpenoids from both two types of samples. In general, there is distinctive difference in phytochemical profiles between these two types of samples. Specifically, 33 phytochemicals showed significant differences. Of these phytochemicals, 22 compounds, such as laetanine, p-coumaroyl-beta-D-glucose, geniposide, medicagenic acid, were significantly higher in cultivated type; 11 compounds, such as vicenin-2, kaempferol 7-neohesperidoside, vanillic acid, and obacunone, were significantly higher in wild-grown type samples. This study will expand our knowledge regarding the cultivated of P. sibiricum and facilitate its further application in pharmaceutical and food industries.
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39

Stevens-Barrón, Jazmín C., Laura A. de la Rosa, Abraham Wall-Medrano, Emilio Álvarez-Parrilla, Roberto Rodríguez-Ramirez, Ramón E. Robles-Zepeda, and Humberto Astiazaran-García. "Chemical Composition and In Vitro Bioaccessibility of Antioxidant Phytochemicals from Selected Edible Nuts." Nutrients 11, no. 10 (September 27, 2019): 2303. http://dx.doi.org/10.3390/nu11102303.

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The ultimate health benefits of peanuts and tree nuts partially depend on the effective gastrointestinal delivery of their phytochemicals. The chemical composition and in vitro bioaccessibility of tocopherols, tocotrienols and phenolic compounds from peanuts and seven tree nuts were evaluated by analytical and chemometric methods. Total fat and dietary fiber (g 100 g−1) ranged from 34.2 (Emory oak acorn) to 72.5 (pink pine nut; PPN) and from 1.2 (PPN) to 22.5 (pistachio). Samples were rich in oleic and linoleic acids (56–87 g 100 g−1 oil). Tocopherols and tocotrienols (mg·kg−1) ranged from 48.1 (peanut) to 156.3 (almond) and 0 (almond, pecan) to 22.1 (PPN) and hydrophilic phenolics from 533 (PPN) to 12,896 (Emory oak acorn); flavonoids and condensed tannins (mg CE.100 g−1) ranged from 142 (white pine nut) to 1833 (Emory oak acorn) and 14 (PPN) to 460 (Emory oak acorn). Three principal components explained 90% of the variance associated with the diversity of antioxidant phytochemicals in samples. In vitro bioaccessibility of tocopherols, tocotrienols, hydrophilic phenolics, flavonoids, and condensed tannins ranged from 11–51%, 16–79%, 25–55%, 0–100%, and 0–94%, respectively. Multiple regression analyses revealed a potential influence of dietary fiber, fats and/or unsaturated fatty acids on phytochemical bioaccessibility, in a structure-specific manner.
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40

Zreen, Zohra, Amjad Hameed, Shumaila Kiran, Tahir Farooq, and Mohammed Suleiman Zaroog. "A Comparative Study of Diospyros malabarica (Gaub) Extracts in Various Polarity-Dependent Solvents for Evaluation of Phytoconstituents and Biological Activities." BioMed Research International 2022 (June 25, 2022): 1–16. http://dx.doi.org/10.1155/2022/4746223.

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Keeping in mind the ascribed repute of Diospyros malabarica (D. malabarica), this investigation was commenced to assess the effect of diverse solvents on extraction yields, phytochemical components and antioxidant capability, and in vitro biological activities of D. malabarica for pharmaceutically active constituents to combat various infections. To screen phytochemicals both qualitatively (flavonoids, terpenoid, saponins, tannins) and quantitatively like total phenolic and flavonoid contents, Diospyros malabarica parts include the following: root, leaves, bark, stem, ripe, and unripe fruit were sequentially extracted with organic solvents such as petroleum ether, dichloromethane, ethyl acetate, ethanol, methanol, and water in increasing order of polarity from less polar to more polar solvents. Furthermore, biological activities such as antibacterial, antifungal, anticancer, antidiabetic, and anti-inflammatory were explored. The results revealed that all the tested solvents displayed a vital role in the extraction yield, the content of phytochemicals, and the studied biological activities. Methanol was found as the best solvent followed by the ethanol for the extraction, representing the highest extraction yield (18.3%), rich diversity of phytochemicals, and the highest total phenolic contents ( 602 ± 0.001 μ g EAG/mg of extract) and total flavonoid contents ( 455 ± 0.6 μ g EQ/mg of extract) in bark extract. Furthermore, methanol bark extract showed high in vitro antibacterial activity ( 30.25 mm ± 0.9 ), antifungal activity ( 18.25 mm ± 0.2 ), anticancer activity (48%), antidiabetic activity (68%) and anti-inflammatory activity (62%) followed by ethanol amongst other extracts of D. malabarica. Accordingly, methanol might be as an ideal solvent to get maximum content of phytochemicals, promising antioxidants, and in vitro biological activities from bark extract amongst other extracts of D. malabarica compared to pet ether, ethyl acetate, and dichloromethane and may act as free radical rummager because phytochemical constituents exhibit antioxidant capability. Our findings suggest that phytochemical compounds (flavonoids, tannins, phenols, saponins, and terpenoids) found in the bark extract of D. malabarica may be attributed to evaluate potent anti-inflammatory, anticancer, antidiabetic, antibacterial, and antifungal activities.
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41

Dienaitė, Lijana, Milda Pukalskienė, Carolina V. Pereira, Ana A. Matias, and Petras Rimantas Venskutonis. "Valorization of European Cranberry Bush (Viburnum opulus L.) Berry Pomace Extracts Isolated with Pressurized Ethanol and Water by Assessing Their Phytochemical Composition, Antioxidant, and Antiproliferative Activities." Foods 9, no. 10 (October 6, 2020): 1413. http://dx.doi.org/10.3390/foods9101413.

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Defatted by supercritical CO2, Viburnum opulus berry pomace (VOP) was subjected to consecutive extraction with pressurized ethanol (E) and water (W) and yielded 23% of VOP-E and 8% of VOP-W, respectively. The major phytochemical groups covering 42 identified and quantified constituents in VOP extracts were organic and phenolic acids, iridoids, quercetin and (epi)catechin derivatives, flavalignans, procyanidins, and anthocyanins. The on-line HPLC-DPPH•-scavenging assay revealed the presence of numerous antioxidants. VOP-E had a higher total phenolic content, was a stronger antioxidant (equivalent to 0.77, 0.42, and 0.17 g trolox/g in oxygen radical absorbance capacity (ORAC), ABTS, and DPPH assays, respectively), and recovered the major part of phenolics from the pomace; however, both extracts demonstrated similar antioxidant activity in the cellular assay. VOP-E inhibited HT29 cancer cells at non-cytotoxic concentrations. The results of this study revealed that VOP contains valuable phytochemicals possessing antioxidant and antiproliferative activities. Consequently, extracts from VOP substances may be of interest in developing functional ingredients for healthy foods, nutraceuticals, and cosmeceuticals.
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42

Rawal, Pooja, Nitisha Negi, Archana N. Sah, and Anchala Guglani. "A Comparative Study of Antioxidant Potential and Phytochemical Contents of different Extracts of Wild Nasturtium Officinale W T Aiton Collected from Kumaun Region of Uttarakhand." Defence Life Science Journal 6, no. 4 (October 5, 2021): 298–304. http://dx.doi.org/10.14429/dlsj.6.16281.

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Nasturtium officinale W. T Aiton or “watercress” (Brassicaceae) is a nutritionally valuable plant that is the richest source of carotenoids, polyphenols, iron, calcium, iodine, folic acid, essential vitamins and minerals. It is traditionally used for their appetiser, anti-scorbutic, stimulant, diuretic and detoxifying properties. The present study was conducted to investigate the antioxidant potential, phytochemical contents (total phenolic, flavonoids and tannin contents) as well as a preliminary phytochemical screening of different extract of aerial parts of Nasturtium officinale collected from the Kumaun region. The different extracts showed significant antioxidant activity as well as total phenolic, flavonoids and tannin contents. The preliminary phytochemical screening showed the presence of several phytochemical constituents such as carbohydrates, proteins, amino acids, glycosides, alkaloids and others. The hydroalcoholic extract possess significantly (P<0.05) higher antioxidant potential with IC50 value (0.333 mg/ml, 0.509mg/ml)and EC50 value (3.537 mg/ml) by 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)(ABTS), and ferric-reducing antioxidant power (FRAP) methods, respectively, as well as highest total tannin, flavonoid and phenolic contents among all the extracts. These results provide substantial evidence that the plant Nasturtium officinalehas antioxidant potential and valuable sources of phytochemicals.
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Castro-Enríquez, Daniela D., Beatriz Montaño-Leyva, Carmen L. Del Toro-Sánchez, Josué E. Juárez-Onofre, Elizabeth Carvajal-Millán, Guadalupe A. López-Ahumada, Carlos G. Barreras-Urbina, José A. Tapia-Hernández, and Francisco Rodríguez-Félix. "Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile." Molecules 25, no. 2 (January 9, 2020): 281. http://dx.doi.org/10.3390/molecules25020281.

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Stenocereus thurberi is an endemic species in northwestern Mexico. It produces colorful fruits called pitayas that have an edible pulp. They have phytochemical compounds associated with biological activities. Ultrafiltration is a widely used method for the clarification of fruit juices and the recovery of phytochemicals. However, its effect has not been extensively studied in extracts. Therefore, the objective of this work is to study the effect of the ultrafiltration of pitaya extract (Stenocereus thurberi) on its phytochemical content, antioxidant capacity, and identification of phenolic compounds by UPLC-DAD-MS, providing greater knowledge about the pitaya. In this study, two extracts were analyzed, the unclarified extract (UE) and the clarified extract (CE). The antioxidant capacity was higher in the CE with 15.93 ± 0.42 mM TE/g, DPPH and 18.37 ± 0.016 mM TE/g, ABTS. The UPLC-MS analysis indicated the decrease in phenolic compounds in the CE and the presence of gallic acid and resorcinol, compounds that had not been identified in other species of Stenocereus spp. The correlation analysis indicated that all the phytochemicals present in the pitaya contribute significantly to the antioxidant capacity. The ultrafiltration process could be a viable option to improve the biological activity of the natural extracts.
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Kiani, Hafiza Sehrish, Akhtar Ali, Shama Zahra, Zain Ul Hassan, Khadija Tul Kubra, Muhammad Azam, and Hafza Fasiha Zahid. "Phytochemical Composition and Pharmacological Potential of Lemongrass (Cymbopogon) and Impact on Gut Microbiota." AppliedChem 2, no. 4 (November 24, 2022): 229–46. http://dx.doi.org/10.3390/appliedchem2040016.

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Phytochemicals are versatile plant secondary metabolites with therapeutic properties. In this review, we explore lemongrass’s phytochemistry and pharmacological potential (Cymbopogon) as well as its impact on gut microbiota. Lemongrass is well-known for its antioxidant, anti-microbial, anti-inflammatory, anti-hypertensive, anti-diabetic, anti-mutagenicity, anxiolytic properties, and for its hypoglycemic and hypolipidemic activities. Therefore, it is widely used in pharmaceuticals, food, feed, and the cosmetics industry. Lemongrass contains phenolic metabolites (including phenolic acids, flavonoids, stilbenes, and lignans), terpenoids, and alkaloids, which are potent bioactive ingredients. Lemongrass is a precious medicinal plant. Furthermore, lemongrass phytochemicals are considered potential agents to improve health by establishing a balanced gut ecosystem. Lemongrass is considered a quintessential food and feed additive at the industrial level, since there are no issues with residue or toxins. Lemongrass powder and essential oils are used to modulate the gut ecosystem by generating anti-microbial, anti-inflammatory, and antioxidant responses, increasing the optimum nutrient absorption in the gut system. This review will further explore lemongrass’s phytochemical, pharmacological, and therapeutic potential.
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45

Gololo, Sechene S., Denga Nthai, and Motetelo A. Mogale. "Phytochemical Contents and Antioxidant Capacity of the Leaves of Aloe greatheadii var. davyana from Four South African Provinces." Asian Journal of Chemistry 31, no. 8 (June 28, 2019): 1830–34. http://dx.doi.org/10.14233/ajchem.2019.22019.

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The current study was aimed at the determination of the phytochemical contents and antioxidant capacity of the leaves of A. greatheadii from four provinces of South Africa. The leaves samples of A. greatheadii were collected at four different locations in South Africa; namely, Limpopo province (Polokwane), Gauteng province (Ga-Rankuwa), Mpumalanga province (Siyabuswa) and North-West province (Brits) during the summer season of the year. The ground samples were then extracted in a serial sequential procedure with different organic solvents using cold maceration extraction method. The extracts were then subjected to quantitative phytochemical analysis for the determination of total phenolic, total tannin, total flavonoid and total saponin content. In addition, the antioxidant capacity of the methanol extracts were evaluated through the DPPH assay. Quantitative phytochemical analysis showed variation in the total phenolic, total tannin, total flavonoid and total saponin contents of the samples from different locations, with specific phytochemicals found in higher amounts at different locations. However, upon calculation of the total phytochemical content the high amount was recorded with the Limpopo province sample. Variation in the antioxidant capacity (EC50 based) of the samples from different locations was demonstrated, with the Limpopo and Mpumalanga provinces’ samples showing high capacity. The results demonstrated that the variation in geographical locations has an effect on the phytochemical contents and antioxidant capacity of the leaves of A. greatheadii. Furthermore, the Limpopo province area with a high altitude appeared to be providing suitable growing conditions for high accumulation of phytochemicals and higher antioxidant capacity.
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46

D. Wadankar, Gajanan, Bharati R. Mahale, Mayuri V. Kute, and Pranali D. Khadse. "Investigation of Phytochemical in Euphorbia heterophylla and Euphorbia rothiana." International Journal of Medical, Pharmacy and Drug Research 6, no. 3 (2022): 16–19. http://dx.doi.org/10.22161/ijmpd.6.3.2.

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The genus Euphorbia attracted the attention of many researchers as it is poorly studied. Euphorbia heterophyla and Euphorbia rothiana were selected and aimed to investigate the presence of phytochemicals screenings in leaf and stem. Aqueous, Acetone and Methanol Solvents were used for phytochemical analysis. The phytochemical analysis reveals the presence of phenolic compound in leaf and stem of the plants in all extracts and flavonoid using aqueous and methanol extracts. Our findings provides evidence, that aqueous and organic solvent extracts of these plants contain medicinally important bioactive compounds.
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Md. Saddam Hussain, Mohammad Salim Hossain, and Md. Mamun Or Rashid. "Antiobesity and Lipid Lowering Activitiy of Vigna unguiculata (L) Walp. Seed in High Fat Diet Induced Obese Mice." Journal of Pharmacy and Nutrition Sciences 9, no. 4 (August 5, 2019): 233–38. http://dx.doi.org/10.29169/1927-5951.2019.09.04.6.

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Seed of Vigna unguiculata is commonly used as culinary in Bangladesh and many parts of the world. Besides this, seed of V. unguiculata possesses some medicinal values like being an antioxidant etc. In this study, seeds of V. unguiculata were used to determine the link between its total phenolic content and lipid lowering activity. Phytochemicals presents in the seeds were evaluated. Total phenolic content (TPC) was determined by using folin-ciocalteu method. High fat diet induced obese mice were used for anti-obesity and antihyperlipidemic test. Body weight gain, total cholesterol and triglyceride levels were analyzed. Preliminary phytochemical screening showed that amino acid, phenol, alkaloid and terpenes were very strongly present in cowpea seed, while carbohydrate, cardiac glycoside, was moderately present. The V. unguiculata seeds showed significant total phenolic content with a value of 119.716mg of GAE/gm equivalent. Cowpea seeds, with a dose of 400mg/kg body-weight, showed significant reduction in body weight gain when treated with fat diet compared with normal diet. Cowpea seeds are also capable of lowering the serum cholesterol (p < 0.01) and serum triglyceride level (p < 0.01) more significantly in high fat diet induced obesity. V. unguiculata seeds have a significantly high total phenolic content while the other phytochemicals are moderately present in them. It has anti-obesity activity that can interfere with serum cholesterol and triglyceride metabolism. So, it seems like a healthy diet for the management of obesity and other heart related disease.
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48

Parasram, Kathyani. "Flavonoids and Diarylheptanoids: Neuroprotective Activities of Phytochemicals." International Journal of Pharmacology, Phytochemistry and Ethnomedicine 6 (January 2017): 82–86. http://dx.doi.org/10.18052/www.scipress.com/ijppe.6.82.

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Plants are often used as sources of lead compounds with phenolic compounds frequently attributed to physiological effects. Flavonoids and diarylheptanoids are important groups of phenolic compounds that impart antioxidant, antitumorgenic, antinflammatory, and neuroprotective effects. These neuroprotective effects can be harnessed to develop treatments for neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease. Recent discoveries have characterized new neuroprotective compounds and/or sources and tested treatments on cell lines and model animals to improve treatments for future persons with neurodegenerative disorders.
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Chang, Xiaoxiao, Yutong Ye, Jianping Pan, Zhixiong Lin, Jishui Qiu, Cheng Peng, Xinbo Guo, and Yusheng Lu. "Comparative Analysis of Phytochemical Profiles and Antioxidant Activities between Sweet and Sour Wampee (Clausena lansium) Fruits." Foods 11, no. 9 (April 25, 2022): 1230. http://dx.doi.org/10.3390/foods11091230.

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As a local medicine and food, wampee fruit, with abundant bioactive compounds, is loved by local residents in Southern China. Titratable acid (TA), total sugar (TS), and total phenolic and flavonoid contents were detected, and phytochemical profiles and cellular antioxidant activities were analyzed by the HPLC and CAA (cellular antioxidant activity) assay in five sweet wampee varieties and five sour wampee varieties. Results showed that the average TS/TA ratio of sweet wampee varieties was 29 times higher than sour wampee varieties, while TA content was 19 times lower than sour wampee varieties. There were much lower levels of total phenolics, flavonoids, and antioxidant activities in sweet wampee varieties than those in sour wampee varieties. Eight phytochemicals were detected in sour wampee varieties, including syringin, rutin, benzoic acid, 2-methoxycinnamic acid, kaempferol, hesperetin, nobiletin, and tangeretin, while just four of them were detected in sweet wampee varieties. Syringin was the only one that was detected in all the sour wampee varieties and was not detected in all sweet wampee varieties. Correlation analysis showed significant positive correlations between TA with phenolics, flavonoids, and total and cellular (PBS wash) antioxidant activities, while there were significant negative correlations between TS/TA with phenolic and cellular (no PBS wash) antioxidant activities. This suggested that the content of titratable acid in wampee fruit might have some relationship with the contents of phenolics and flavonoids. Sour wampee varieties should be paid much attention by breeders for their high phytochemical contents and antioxidant activities for cultivating germplasms with high health care efficacy.
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50

Chang, Xiaoxiao, Yutong Ye, Jianping Pan, Zhixiong Lin, Jishui Qiu, Cheng Peng, Xinbo Guo, and Yusheng Lu. "Comparative Analysis of Phytochemical Profiles and Antioxidant Activities between Sweet and Sour Wampee (Clausena lansium) Fruits." Foods 11, no. 9 (April 25, 2022): 1230. http://dx.doi.org/10.3390/foods11091230.

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Abstract:
As a local medicine and food, wampee fruit, with abundant bioactive compounds, is loved by local residents in Southern China. Titratable acid (TA), total sugar (TS), and total phenolic and flavonoid contents were detected, and phytochemical profiles and cellular antioxidant activities were analyzed by the HPLC and CAA (cellular antioxidant activity) assay in five sweet wampee varieties and five sour wampee varieties. Results showed that the average TS/TA ratio of sweet wampee varieties was 29 times higher than sour wampee varieties, while TA content was 19 times lower than sour wampee varieties. There were much lower levels of total phenolics, flavonoids, and antioxidant activities in sweet wampee varieties than those in sour wampee varieties. Eight phytochemicals were detected in sour wampee varieties, including syringin, rutin, benzoic acid, 2-methoxycinnamic acid, kaempferol, hesperetin, nobiletin, and tangeretin, while just four of them were detected in sweet wampee varieties. Syringin was the only one that was detected in all the sour wampee varieties and was not detected in all sweet wampee varieties. Correlation analysis showed significant positive correlations between TA with phenolics, flavonoids, and total and cellular (PBS wash) antioxidant activities, while there were significant negative correlations between TS/TA with phenolic and cellular (no PBS wash) antioxidant activities. This suggested that the content of titratable acid in wampee fruit might have some relationship with the contents of phenolics and flavonoids. Sour wampee varieties should be paid much attention by breeders for their high phytochemical contents and antioxidant activities for cultivating germplasms with high health care efficacy.
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