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Journal articles on the topic 'Phenotyping and genotyping of serratia marcescens'

Author: Grafiati
Published: 3 June 2025
Last updated: 31 July 2025

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1

Zhang, Q., R. Weyant, A. G. Steigerwalt, et al. "Genotyping of Serratia marcescens Strains Associated with Cucurbit Yellow Vine Disease by Repetitive Elements-Based Polymerase Chain Reaction and DNA-DNA Hybridization." Phytopathology® 93, no. 10 (2003): 1240–46. http://dx.doi.org/10.1094/phyto.2003.93.10.1240.

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The bacterium that causes cucurbit yellow vine disease (CYVD) has been placed in the species Serratia marcescens based on 16S rDNA and groE sequence analysis. However, phenotypic comparison of the organism with S. marcescens strains isolated from a variety of ecological niches showed significant heterogeneity. In this study, we compared the genomic DNA of S. marcescens strains from different niches as well as type strains of other Serratia spp. through repetitive elements-based polymerase chain reaction (rep-PCR) and DNA-DNA hybridization. With the former, CYVD strains showed identical banding
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2

Garza-González, Elvira, Sandra Iveth Mendoza Ibarra, Jorge M. Llaca-Díaz та Gloria M. Gonzalez. "Molecular characterization and antimicrobial susceptibility of extended-spectrum β-lactamase-producing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico". Journal of Medical Microbiology 60, № 1 (2011): 84–90. http://dx.doi.org/10.1099/jmm.0.022970-0.

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Our objective was to analyse phenotypic and genetic data of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae, Enterobacter cloacae, Escherichia coli and Serratia marcescens that cause infections in our hospital. Over a 3 year period, 342 randomly selected clinical Enterobacteriaceae isolates were tested for ESBL production and evaluated for the presence of the β-lactamase genes bla SHV, bla TEM, bla CTX-M and bla TLA-1. The antibiotic susceptibilities of these isolates were also determined, and the clonality of the isolates was assessed by PFGE. Based on our analyses, 33/92
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3

Bhadra, Bhaskar, Pradosh Roy, and Ranadhir Chakraborty. "Serratia ureilytica sp. nov., a novel urea-utilizing species." International Journal of Systematic and Evolutionary Microbiology 55, no. 5 (2005): 2155–58. http://dx.doi.org/10.1099/ijs.0.63674-0.

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A Gram-negative, rod-shaped, urea-dissolving and non-spore-forming bacterium, designated strain NiVa 51T, was isolated from water of the River Torsa in Hasimara, Jalpaiguri district, West Bengal, India. On the basis of 16S rRNA gene sequence similarity, strain NiVa 51T was shown to belong to the γ-Proteobacteria and to be related to Serratia marcescens subsp. sakuensis (98·35 %) and S. marcescens subsp. marcescens (98·30 %); however, strain NiVa 51T exhibited only 43·7 % similarity to S. marcescens by DNA–DNA hybridization. The G+C content of the genomic DNA of the isolate was 60 mol%. Both bi
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4

Abid, Suhad Abbas, Israa M. S. Al-Kadmy, Sarah Naji Aziz, and Israa H. Hamzah. "The predominance of virulence genes associated with multidrug-resistant Serratia marcescens isolated from urinary tract infections." Reviews and Research in Medical Microbiology 35, no. 3 (2023): 160–66. http://dx.doi.org/10.1097/mrm.0000000000000343.

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Purpose: Serratia marcescens owns different virulence factors that contribute to their pathogenesis and result in bacterial invasion and resistance. Moreover, patients who suffer from urinary tract infections (UTIs) are at an increased risk of contracting different bacterial infections. This study aimed to detect and verify the occurrence of virulence genes in S. marcescens isolated from patients with UTIs in some hospitals in Iraq. Methodology: After bacterial collection, the identification was achieved by busing phenotypic and genotypic methods. The antibiotic susceptibility patterns were do
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5

Lima, Karla Valéria Batista, Raimundo Gladson Corrêa Carvalho, Irna Carla do Rosário Souza Carneiro, et al. "Outbreak of neonatal infection by an endemic clone of Serratia marcescens." Revista da Sociedade Brasileira de Medicina Tropical 44, no. 1 (2011): 106–9. http://dx.doi.org/10.1590/s0037-86822011000100024.

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INTRODUCTION: The outbreak occurred between February and June 2006 and included identification of the cases, analysis of medical records, cultures from environmental sources, resistance analyses and genotyping profile of Serratia marcescens. METHODS: The cultures were composed of 13 blood isolates, 17 rectal and hand swabs and air sampling. RESULTS: The data obtained by pulsed-field gel electrophoresis exhibited three strains that contaminated 24 patients. Systemic infection was the most common in neonates with lower weight, long periods of hospitalization, premature delivery and the use of me
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6

Morandi, Stefano, Valentina Pica, Fabio Masotti, et al. "Proteolytic Traits of Psychrotrophic Bacteria Potentially Causative of Sterilized Milk Instability: Genotypic, Phenotypic and Peptidomic Insight." Foods 10, no. 5 (2021): 934. http://dx.doi.org/10.3390/foods10050934.

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The proteolytic traits of the psychrotrophic strains Pseudomonas poae LP5, Pseudomonas fluorescens LPF3, Chryseobacterium joostei LPR1, Pseudomonas fulva PS1, Citrobacter freundii PS37, Hafnia alvei PS46, and Serratia marcescens PS92 were initially investigated by phenotypic and genotypic approaches. Six strains elicited extracellular proteolytic activity, and five expressed the thermostable AprX or (likely) Ser1 enzymes. Then, the strains were inoculated (104 CFU/mL) in microfiltered pasteurized milk and kept at 4 °C for five days. All of the strains reached 108 CFU/mL at the end of storage a
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7

Cruz, Thalita PPS, Francisco KSF Azevedo, Marco A. Pepato, et al. "Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil." Journal of Infection in Developing Countries 18, no. 05 (2024): 726–31. http://dx.doi.org/10.3855/jidc.18913.

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Introduction: Serratia marcescens is an opportunistic pathogen found ubiquitously in the environment and associated with a wide range of nosocomial infections. This multidrug-resistant bacterium has been a cause of concern for hospitals and healthcare facilities due to its ability to spread rapidly and cause outbreaks. Next generation sequencing genotyping of bacterial isolates has proven to be a valuable tool for tracking the spread and transmission of nosocomial infections. This has allowed for the identification of outbreaks and transmission chains, as well as determining whether cases are
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8

Prado, Gladys, Elisa Teixeira Mendes, Roberta Cristina Ruedas Martins, et al. "Phenotypic and genotypic characteristics of a carbapenem-resistant Serratia marcescens cohort and outbreak: describing an opportunistic pathogen." International Journal of Antimicrobial Agents 59, no. 1 (2022): 106463. http://dx.doi.org/10.1016/j.ijantimicag.2021.106463.

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9

Sarwar, Amna, Syeda Zahra Gillani, Rana Muhammad Shahbakht та ін. "Detection of Β-Lactamase OXA Genes and Biofilm Production in Carbapenem Resistant Gram-Negative Bacilli". Pakistan Journal of Medical and Health Sciences 17, № 4 (2023): 235–38. http://dx.doi.org/10.53350/pjmhs2023174235.

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Objectives: The emergence of carbapenem resistance in biofilm producer Gram-negative bacteria poses a substantial challenge to manage infections. This study aimed to evaluate biofilm production and molecular characterization of carbapenem resistance Gram-negative rods. Methods: Total of 46 bacteria were isolated from various clinical samples, including blood, CSF, urine, sputum, saliva, tracheal secretion, and pus. Following the standard guidelines for bacterial identification (CLSI), bacterial pathogens are isolated and identified. The antibiotic susceptibility profile was obtained against co
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10

Khalil, Nadim, Eleanor Powell, and Joel Mortensen. "663. Improved Detection of ESBL and AmpC Beta-Lactamase Producing Isolates of Enterobacteriaceae in Pediatric Patients with Bloodstream Infections Using Combined Genotypic and Phenotypic Antimicrobial Susceptibility Testing." Open Forum Infectious Diseases 7, Supplement_1 (2020): S387. http://dx.doi.org/10.1093/ofid/ofaa439.856.

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Abstract Background Infections secondary to pathogens resistant to third-generation cephalosporins (3GC), such as extended-spectrum (ESBL) and AmpC β-lactamase (AmpC) producing Enterobacteriaceae, are increasing. Currently, there are no recommendations regarding identification of AmpC in Citrobacter, Enterobacter, Morganella and Serratia spp. (CEMS organisms). This study’s aim was to increase the detection of AmpC and ESBL producing Enterobacteriaceae in blood cultures from pediatric population by combining genotypic with phenotypic antimicrobial susceptibility testing (AST). Methods All first
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Oluwajide, O. O., R. A. Olowe, O. J. Adefioye, et al. "Phenotypic and Genotypic Detection of UV-induced Bacteriocin Production in Enteric Gram-Negative Rods and their Activity against Escherichia coli O157: H7." International Journal of TROPICAL DISEASE & Health 45, no. 6 (2024): 21–31. http://dx.doi.org/10.9734/ijtdh/2024/v45i61536.

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Background: Virulence and antibiotic resistance of bacterial pathogens are one of the most universal threats to public health care. 
 Materials: In this study, a total of two hundred and fifty-seven (257) enteric bacteria isolated from human rectal swabs and cattle dung were screened for production of low-toxic, high target-specific bactericidal macromolecule peptides (Bacteriocin) against strains of E. coli 0157: H7 using agar well diffusion method. The producer isolates were exposed to ultra-violet (UV) rays (UV 302nm and 254nm) for induction.
 Results: Out of the 257 isolates, 21
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12

Bekenniche, Nahla, Ait Abdeslam Arezki, Nemmiche Saïd, Ait Saada Djamal, Cheriguenen Abderrahim, and Bekada Ahmed Mohammed Ali. "Molecular identification of crude oil-degrading bacteria isolated from polluted sites in Algeria." South Asian Journal of Experimental Biology 11, no. 2 (2021): 164–71. http://dx.doi.org/10.38150/sajeb.11(2).p164-171.

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The petroleum industry in Algeria takes a predominant place in the econo-my. This industry amplifies the environmental contamination and affects microbial diversity of soils. Bioremediation is a cost-effective and efficient process for removing hydrocarbon pollutants. The aim of the study was to isolate cultivable telluric bacteria and to assess their potential as bioremedi-ation agents against the contamination of petroleum hydrocarbons. Crude oil polluted soil samples were taken from different oil refinery in Algeria. Elemental analysis was determined by inductively coupled plasma optical em
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13

Barbosa, F. C. B., K. Irino, G. V. Carbonell, and M. P. A. Mayer. "Characterization of Serratia marcescens isolates from subgingival biofilm, extraoral infections and environment by prodigiosin production, serotyping, and genotyping." Oral Microbiology and Immunology 21, no. 1 (2006): 53–60. http://dx.doi.org/10.1111/j.1399-302x.2005.00254.x.

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14

Főldes, Annamária, Doina-Veronica Bilca, and Edit Székely. "Phenotypic and molecular identification of carbapenemase-producing Enterobacteriaceae - challenges in diagnosis and treatment." Revista Romana de Medicina de Laborator 26, no. 2 (2018): 221–30. http://dx.doi.org/10.2478/rrlm-2018-0018.

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Abstract Introduction: Infections due to carbapenemase-producing carbapenem-resistant Enterobacteriaceae (CPCRE) are an emerging global public health threat. The purpose of this study was to investigate phenotypic and genotypic features of CP-CRE strains isolated from hospitalized patients. Material and methods: Between 1st of January - 1st of July 2017, in the Department of Microbiology, “Dr. Constantin Opriş” County Emergency Hospital Baia Mare, Romania, 1110 strains of Enterobacteriaceae were isolated from bronchial secretions, urine, wounds and blood cultures. Bacterial identification and
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15

Barbosa, Thaís Alves, Maria Regina Bentlin, Lígia Maria Suppo de Souza Rugolo, et al. "Molecular Characterization of Gram-Negative Bacilli Isolated from a Neonatal Intensive Care Unit and Phenotypic and Molecular Detection of ESBL and Carbapenemase." Antibiotics 14, no. 4 (2025): 342. https://doi.org/10.3390/antibiotics14040342.

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Introduction: The increase in the rates of multidrug-resistant bacteria in healthcare environments has been recognized as a global public health problem. In view of the scarcity of data on the neonatal population, this study aimed to provide information on the genotypic and epidemiological characteristics of Gram-negative microorganisms isolated from colonization and infection sites in neonates admitted to a tertiary university center of high complexity. Methods: Enterobacterales and non-fermenting Gram-negative bacilli previously collected in a prospective cohort study were submitted to genot
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16

Amaretti, Alberto, Lucia Righini, Francesco Candeliere, et al. "Antibiotic Resistance, Virulence Factors, Phenotyping, and Genotyping of Non-Escherichia coli Enterobacterales from the Gut Microbiota of Healthy Subjects." International Journal of Molecular Sciences 21, no. 5 (2020): 1847. http://dx.doi.org/10.3390/ijms21051847.

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Non-Escherichia coli Enterobacterales (NECE) can colonize the human gut and may present virulence determinants and phenotypes that represent severe heath concerns. Most information is available for virulent NECE strains, isolated from patients with an ongoing infection, while the commensal NECE population of healthy subjects is understudied. In this study, 32 NECE strains were isolated from the feces of 20 healthy adults. 16S rRNA gene sequencing and mass spectrometry attributed the isolates to Klebsiella pneumoniae, Klebsiella oxytoca, Enterobacter cloacae, Enterobacter aerogenes, Enterobacte
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17

LI, LILI, RIKKE HEIDEMANN OLSEN, LEI YE, et al. "Antimicrobial Resistance and Resistance Genes in Aerobic Bacteria Isolated from Pork at Slaughter." Journal of Food Protection 79, no. 4 (2016): 589–97. http://dx.doi.org/10.4315/0362-028x.jfp-15-455.

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ABSTRACT The aim of this study was to investigate the phenotypic and genotypic antimicrobial resistance, integrons, and transferability of resistance markers in 243 aerobic bacteria recovered from pork at slaughter in the People's Republic of China. The organisms belonged to 22 genera of gram-negative bacteria (92.2%) and gram-positive bacteria (7.8%). High levels of resistance were detected to tetracycline, trimethoprim-sulfamethoxazole, and ampicillin (36.2 to 54.3%), and lower levels were detected to nitrofurantoin, cefotaxime, gentamicin, ciprofloxacin, and chloramphenicol (7.8 to 29.2%).
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18

Mohd Khalif, Saldatul As Wani, Nurul Zahidah Nordin, and Nadiawati Alias. "Identification and characterization of potential compost degrading bacteria from agro-waste." Malaysian Journal of Fundamental and Applied Sciences 17, no. 1 (2021): 26–32. http://dx.doi.org/10.11113/mjfas.v17n1.1924.

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Agricultural industry plays a significant role in the global economic growth. It is estimated more than 15% of total waste in Asia are contributed by agro waste. Bacteria is known as one of the useful organisms actively found to surround the waste industry. They are considered as chemical decomposers and act as driving agent of composting that changes the chemistry of organic waste to simple compounds. Thus, this study was conducted to isolate and identify potential compostdegrading bacteria from agriculture waste at several sampling areas in Besut district, Terengganu, Malaysia. A total of 49
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Herpich, Claudemar Helmuth, Leocir José Welter, Glória Regina Botelho, Fábio Nascimento da Silva, Ricardo Trezzi Casa, and Amauri Bogo. "Epiphytic and endophytic bacteria for the control of Botrytis cinerea in vitro and in grape berries of cv. sauvignon blanc in southern Brazil." Bioscience Journal 41 (February 12, 2025): e41005. https://doi.org/10.14393/bj-v41n0a2025-74859.

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Botrytis bunch rot (BBR) in grapevines caused by Botrytis cinerea is a pre- and post-harvest disease that leads to significant losses in southern Brazil. This study aimed to assess the potential of epiphytic and endophytic bacteria to control B. cinerea in vitro and in vivo in the cv. Sauvignon Blanc (SB). B. cinerea and epiphytic and endophytic bacterial isolates were obtained from the grape clusters and leaf phyllosphere (epiphytic and endophytic) of the SB variety, respectively. Four epiphytic and two endophytic isolates that showed the highest antibiosis indices in vitro and in vivo were i
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20

Parvaz, P., D. Tille, H. Meugnier, et al. "A rapid and easy PCR-RFLP method for genotyping Serratia marcescens strains isolated in different hospital outbreaks and patient environments in the Lyon area, France." Journal of Hospital Infection 51, no. 2 (2002): 96–105. http://dx.doi.org/10.1053/jhin.2002.1224.

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21

Loncaric, Igor, Dusan Misic, Michael P. Szostak, Frank Künzel, Sabine Schäfer-Somi, and Joachim Spergser. "Broad-Spectrum Cephalosporin-Resistant and/or Fluoroquinolone-Resistant Enterobacterales Associated with Canine and Feline Urogenital Infections." Antibiotics 9, no. 7 (2020): 387. http://dx.doi.org/10.3390/antibiotics9070387.

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The aim of the present study was to characterize Enterobacterales resistant to 3rd and 4th generation cephalosporins, carbapenems and/or fluoroquinolones, isolated from dogs and cats with urogenital infections. In total, 36 strains (Escherichia coli (n = 28), Klebsiella pneumoniae (n = 3), Serratia marcescens, Raoultella ornithinolytica, Proteus mirabilis, Citrobacter portucalensis and Enterobacter cloacae (each n = 1)) were included in the present study, 28 from Austria and 8 from Serbia. Isolates were characterized by a polyphasic approach including susceptibility pheno- and genotyping and m
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22

Trivedi, Mahendra Kumar, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, and Snehasis Jana. "Evaluation of Phenotyping and Genotyping Characterization of Serratia marcescens after Biofield Treatment." Journal of Molecular and Genetic Medicine 9, no. 3 (2015). https://doi.org/10.4172/1747-0862.1000179.

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Serratia marcescens (S. marcescens) is Gram-negative bacterium, associated with hospital-acquired infections (HAIs), especially urinary tract and wound infections. The present study was aimed to evaluate the impact of biofield treatment on phenotyping and genotyping characteristics such as antimicrobial susceptibility, biochemical reactions, biotype, DNA polymorphism, and phylogenetic relationship of S. marcescens (ATCC 13880). The lyophilized cells of S. marcescens were divided into three groups (G1, G2, and G3). Control group (G1) and treated groups (G2 and G3) of S. Marcescens cells assesse
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23

Trivedi, Mahendra. "Evaluation of Phenotyping and Genotyping Characterization of Serratia Marcescens after Biofield Treatment." OMICS International, August 31, 2015. https://doi.org/10.5281/zenodo.813384.

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Serratia marcescens (S. marcescens) is Gram-negative bacterium, associated with hospital-acquired infections (HAIs), especially urinary tract and wound infections. The present study was aimed to evaluate the impact of biofield treatment on phenotyping and genotyping characteristics such as antimicrobial susceptibility, biochemical reactions, biotype, DNA polymorphism, and phylogenetic relationship of S. marcescens (ATCC 13880). The lyophilized cells of S. marcescens were divided into three groups (G1, G2, and G3). Control group (G1) and treated groups (G2 and G3) of S. marcescens cells assesse
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24

Patil S, Trivedi MK, and Shettigar H. Bairwa K. "Evaluation of Phenotyping and Genotyping Characterization of Serratia marcescens after Biofield Treatment." Journal of Molecular and Genetic Medicine 09, no. 03 (2015). http://dx.doi.org/10.4172/1747-0862.1000179.

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25

Sadeq, Jenan Nadhim, and Ahmed Jasim Neamah. "Comparative Phenotypic and Genotypic Properties of Serratia marcescens Isolates from Clinical Cases from human in AL-Diwaniyah Province, Iraq." South Eastern European Journal of Public Health, October 24, 2024, 580–92. http://dx.doi.org/10.70135/seejph.vi.1790.

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Serratia marcescens is a Gram-negative, rod-shaped bacterium. It is an opportunistic and facultative anaerobe. This study aims to determine the occurrence rates of Serratia marcescens in human clinical samples, investigate the pigM gene, sequence the isolates, and compare them with global isolates documented in the NCBI database. Fifty burn samples were collected from the specialized center for burns in AL-Diwaniyah province, along with 35 urine samples from individuals suffering from UTIs from Al-Diwaniyah Teaching Hospital and various private laboratories. These samples were transferred in a
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26

N., Gayathri, and R. Subashkumar. "Phenotypic and Genotypic Characterization of Serratia marcescens from Clinical and Environmental Sources." International Journal of Pharmacognosy and Phytochemical Research 9, no. 11 (2018). http://dx.doi.org/10.25258/phyto.v9i11.11180.

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S.marcescens is opportunistic pathogens that cause nosocomical infections. There have been many reports concerning the identification, antibiotic susceptibility, pathogenicity, epidemiological investigations and typing of this organism. Accurate identification is important in defining outbreaks. In the present study, S. marcescens have been selected from the natural sources and were then confirmed with 16S rRNA identification method with cultures of MTCC. To investigate these S. marcescens isolates, random amplification of polymorphic DNA (RAPD) was used in conjunction with the specific primer
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27

KUSUMA, SRI AGUNG FITRI, VALENTINA YURINA, DEBBIE S. RETNONINGRUM, INDAH LAILY HILMI, SUSI AFRIANTI RAHAYU та YUNI NOER ANGGRAINI. "Β-LACTAMASES INHIBITOR-PRODUCING SOIL BACTERIA FOR AMPICILLIN-RESISTANT UROPATHOGENIC ESCHERICHIA COLI ISOLATE". International Journal of Applied Pharmaceutics, 27 грудня 2022, 40–44. http://dx.doi.org/10.22159/ijap.2022.v14s5.03.

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Objective: The goals of this investigation were to identify the species of the producers and ascertain the dose-dependent effect of extracellular products of Indonesian bacteria that generate β-lactamases inhibitors.
 Methods: An agar diffusion technique for the lactamase inhibitor activity assay was performed. Observation of bacteria using phenotypic analysis was performed by observing colony color and cell shape morphology, biochemical assays and a series of carbohydrate fermentation tests. Bacterial identification was performed by comparing the nucleotide sequence of the 16S rDNA gene
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28

Hopkins, Heather A., Christian Lopezguerra, Meng-Jia Lau, and Kasie Raymann. "Making a pathogen? Evaluating the impact of protist predation on the evolution of virulence in Serratia marcescens." Genome Biology and Evolution, July 4, 2024. http://dx.doi.org/10.1093/gbe/evae149.

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Abstract Opportunistic pathogens are environmental microbes that are generally harmless and only occasionally cause disease. Unlike obligate pathogens, the growth and survival of opportunistic pathogens does not rely on host infection or transmission. Their versatile lifestyles make it challenging to decipher how and why virulence has evolved in opportunistic pathogens. The Coincidental Evolution Hypothesis (CEH) postulates that virulence results from exaptation or pleiotropy, i.e., traits evolved for adaptation to living in one environment that have a different function in another. In particu
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Saduakhasova, Saule, Almagul Kushugulova, Gulnara Shakhabayeva, et al. "Lactobacillus for Vaginal Microflora Correction." Central Asian Journal of Global Health 3 (December 12, 2014). http://dx.doi.org/10.5195/cajgh.2014.171.

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Introduction. Despite the significant progress made in prevention, diagnosis, and treatment, there is still a high rate of vaginal dysbiosis in Kazakh women. The use of antibiotics in the treatment of vaginal dysbiosis contributes to the elimination of pathogens as well as microflora, which can lead to a decrease in local immunity and more favorable conditions for infection spread. The most physiologically safe and promising method for the restoration of vaginal biocenosis is the use of probiotics administered by a vaginal route.Methods. We have allocated 64 of cultures of Lactobacillus from t
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Parsons, Joshua B., Andrew Bock, Blake M. Hanson, et al. "152. Molecular Epidemiological Analysis of Relapsed Gram-negative Bloodstream Infection Isolates." Open Forum Infectious Diseases 9, Supplement_2 (2022). http://dx.doi.org/10.1093/ofid/ofac492.230.

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Abstract Background Gram-negative bacterial bloodstream infections (GNB-BSI) are common and frequently lethal. Many patients experience multiple episodes of GNB-BSI for unclear reasons. This study examines the genetic differences between initial and subsequent isolates of recurrent GNB-BSI. Methods We used a prospective cohort of patients with GNB-BSI at Duke Hospital to identify patients with >1 episode of GNB-BSI due to the same bacterial species. We performed pulsed field gel electrophoresis (PFGE) on paired isolates to determine if the events were Reinfection (paired isolates differ
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