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Journal articles on the topic 'Phenylmethylsulfonyl fluoride; Serine protease'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Lopez-Llorca, L. V. "Purification and properties of extracellular proteases produced by the nematophagous fungus Verticillium suchlasporium." Canadian Journal of Microbiology 36, no. 8 (1990): 530–37. http://dx.doi.org/10.1139/m90-093.

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The fungal parasite of eggs of cyst nematodes, Verticillium suchlasporium, produced extracellular proteases when grown in semiliquid culture with gelatin as the only source of nitrogen and carbon. The proteolytic activity of culture filtrates was maximum 12–14 days after inoculation. Gel filtration chromatography in Sephadex G-100 resolved two peaks of proteolytic activity. The peak accounting for most of the activity was further purified by ion-exchange chromatography in SP-Sephadex C-25 as a single peak. This protease had a molecular mass of 32 kDa calculated by sodium dodecyl sulfate – poly

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2

Manavalan, Tamilvendan, Arulmani Manavalan, Shiyamsundar Ramachandran, and Klaus Heese. "Identification of a Novel Thermostable Alkaline Protease from Bacillus megaterium-TK1 for the Detergent and Leather Industry." Biology 9, no. 12 (2020): 472. http://dx.doi.org/10.3390/biology9120472.

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An increased need by the green industry for enzymes that can be exploited for eco-friendly industrial applications led us to isolate and identify a unique protease obtained from a proteolytic Bacillus megaterium-TK1 strain from a seawater source. The extracellular thermostable serine protease was processed by multiple chromatography steps. The isolated protease displayed a relative molecular weight (MW) of 33 kDa (confirmed by zymography), optimal enzyme performance at pH 8.0, and maximum enzyme performance at 70 °C with 100% substrate specificity towards casein. The proteolytic action was blo

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3

Zaqueo, Kayena D., Anderson M. Kayano, Rodrigo Simões-Silva, et al. "Isolation and Biochemical Characterization of a New Thrombin-Like Serine Protease fromBothrops pirajaiSnake Venom." BioMed Research International 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/595186.

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This paper presents a novel serine protease (SP) isolated fromBothrops pirajai, a venomous snake found solely in Brazil that belongs to the Viperidae family. The identified SP, named BpirSP-39, was isolated by three chromatographic steps (size exclusion, bioaffinity, and reverse phase chromatographies). The molecular mass of BpirSP-39 was estimated by SDS-PAGE and confirmed by mass spectrometry (39,408.32 Da). The protein was able to form fibrin networks, which was not observed in the presence of serine protease inhibitors, such as phenylmethylsulfonyl fluoride (PMSF). Furthermore, BpirSP-39 p

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4

Deane, D., L. Inglis, and D. Haig. "The 175 antigen expressed on myeloid and erythroid cells during differentiation is associated with serine protease activity." Blood 85, no. 5 (1995): 1215–19. http://dx.doi.org/10.1182/blood.v85.5.1215.bloodjournal8551215.

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Monoclonal antibody 175 recognizes a cell-surface antigen on more than 80% of nucleated ovine bone marrow cells (BMC). The distribution is unusual, as the majority of differentiated myeloid and erythroid cells express the antigen (175 antigen), whereas mast cells, basophils, and the majority of lymphocytes do not. The level of 175 antigen expression has been shown to increase as BMC differentiate during hematopoiesis. Previous attempts to identify the 175 antigen have been unsuccessful. In this study, the 175 antigen was affinity-purified and shown to contain serine protease activity. Immunobl

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Wang, Wei, Guoqing Peng, Jingjing Sun, Chengcheng Jiang, Jianhua Hao, and Xiu Zhang. "Screening and Characterization of Marine Bacillus atrophaeus G4 Protease and Its Application in the Enzymatic Hydrolysis of Sheep (Ovis aries) Placenta for the Preparation of Antioxidant Peptides." Molecules 30, no. 10 (2025): 2217. https://doi.org/10.3390/molecules30102217.

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Proteolytic enzymes, which play a crucial role in peptide bond cleavage, are widely applied in various industries. In this study, protease-producing bacteria were isolated and characterized from marine sediments collected from the Yellow Sea, China. Comprehensive screening and 16S rDNA sequencing identified a promising G4 strain as Bacillus atrophaeus. Following meticulous optimization of fermentation conditions and medium composition via response surface methodology, protease production using strain G4 was significantly enhanced by 64%, achieving a yield of 3258 U/mL. The G4 protease exhibite

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6

Buckmaster, M. J., J. A. Curci, P. R. Murray, et al. "Source of Elastin-Degrading Enzymes in Mycotic Aortic Aneurysms: Bacteria or Host Inflammatory Response?" Cardiovascular Surgery 7, no. 1 (1999): 16–26. http://dx.doi.org/10.1177/096721099900700106.

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Elastolytic matrix metalloproteinases play a central role in the development of chronic atherosclerotic aortic aneurysms, but mycotic aortic aneurysms are a distinct and unusual form of aneurysm disease caused by bacterial infection. Mycotic aortic aneurysms follow a more rapid and unpredictable course than chronic aneurysm disease and they exhibit a predilection for the suprarenal aorta, further implying unique pathophysiologic mechanisms. The purpose of this study was to examine the nature and source of elastin-degrading enzymes in mycotic aortic aneurysm. Bacterial isolates and aortic tissu

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7

Khan, Hidayatullah, Irshad Ali, Arif-ullah Khan, et al. "Purification and Biochemical Characterization of Alkaline Serine Protease from Caesalpinia bonducella." Natural Product Communications 5, no. 6 (2010): 1934578X1000500. http://dx.doi.org/10.1177/1934578x1000500625.

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A high molecular weight serine protease has been purified to electrophoretic homogeneity from the seeds of Caesalpinia bonducella Flem. (Caesalpiniaceae) by the combination of size exclusion and ion exchange chromatography. About 524 fold purification was achieved with an overall recovery of 6.8%. The specific activity was found to be 86 U/mg/min at pH 8.0. The calculated Km and Vmax were 1.66 mg/mL and 496.68 units/min per mg of protein, respectively. The molecular mass was estimated to be about 63 kDa by sodium dodecyl sulfate PAGE. The enzyme showed optimum activity at pH 8.0 and exhibited

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8

Khan, Naveed A., Edward L. Jarroll, Noorjahan Panjwani, Zhiyi Cao, and Timothy A. Paget. "Proteases as Markers for Differentiation of Pathogenic and Nonpathogenic Species ofAcanthamoeba." Journal of Clinical Microbiology 38, no. 8 (2000): 2858–61. http://dx.doi.org/10.1128/jcm.38.8.2858-2861.2000.

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Acanthamoeba keratitis is a vision-threatening infection caused by pathogenic species of the genusAcanthamoeba. Although not allAcanthamoeba spp. can cause keratitis, it is important to differentiate pathogenic species and isolates from nonpathogens. Since extracellular proteases may play a role in ocular pathology, we used colorimetric, cytopathic, and zymographic assays to assess extracellular protease activity in pathogenic and nonpathogenicAcanthamoeba. Colorimetric assays, using azo-linked protein as a substrate, showed extracellular protease activity in Acanthamoeba-conditioned medium an

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9

Ajamhassani, Maryam, Arash Zibaee, Jalal Sendi, Hassan Askary, and Nasser Farrar. "Proteolytic Activity in the Midgut of the Crimson Speckled Moth Utethesia Pulchella L. (Lepidoptera: Arctiidae)." Journal of Plant Protection Research 52, no. 3 (2012): 368–73. http://dx.doi.org/10.2478/v10045-012-0061-0.

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Proteolytic Activity in the Midgut of the Crimson Speckled MothUtethesia PulchellaL. (Lepidoptera: Arctiidae)Samples were prepared from the midgut of 4th instar larvae of the crimson speckled mothUtethesia pulchellaL. to find proteolytic activity and properties. Result revealed the presence of high proteolytic activity in the midgut when taking into account specific proteinases including trypsin-like, chymotrypsin-like, elastase and two exopeptidase (aminopeptidase and carboxipeptidase). The optimal pH of general protease was 8 and 7 when using azocasein and hemoglobin as general substrates, r

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10

Cui, Hongxia, Liping Wang, and Yang Yu. "Production and Characterization of Alkaline Protease from a High Yielding and Moderately Halophilic Strain of SD11 Marine Bacteria." Journal of Chemistry 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/798304.

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A marine bacterium SD11, which was isolated from sea muds (Geziwo Qinhuangdao Sea area, China), was used to produce thermostable alkaline serine nonmetal protease in the skim milk agar plate medium with 10% NaCl. The optimal temperature about the manufacture of the extracellular protease was ~60°C. The crude enzyme was stable at 20–50°C. The activity was retained to 60% and 45% after heating for 1 h at 60 and 70°C, respectively. The protease was highly active in a wide pH scope (8.0–10.0) and maximum protease activity exhibited at pH 10.0. The activity was restrained by phenylmethylsulfonyl fl

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11

Morimoto-Kamata, Riyo, Sei-ichiro Mizoguchi, Takeo Ichisugi, and Satoru Yui. "Cathepsin G Induces Cell Aggregation of Human Breast Cancer MCF-7 Cells via a 2-Step Mechanism: Catalytic Site-Independent Binding to the Cell Surface and Enzymatic Activity-Dependent Induction of the Cell Aggregation." Mediators of Inflammation 2012 (2012): 1–13. http://dx.doi.org/10.1155/2012/456462.

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Neutrophils often invade various tumor tissues and affect tumor progression and metastasis. Cathepsin G (CG) is a serine protease secreted from activated neutrophils. Previously, we have shown that CG induces the formation of E-cadherin-mediated multicellular spheroids of human breast cancer MCF-7 cells; however, the molecular mechanisms involved in this process are unknown. In this study, we investigated whether CG required its enzymatic activity to induce MCF-7 cell aggregation. The cell aggregation-inducing activity of CG was inhibited by pretreatment of CG with the serine protease inhibito

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12

Clarke, Simon R., and Simon J. Foster. "IsdA Protects Staphylococcus aureus against the Bactericidal Protease Activity of Apolactoferrin." Infection and Immunity 76, no. 4 (2008): 1518–26. http://dx.doi.org/10.1128/iai.01530-07.

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ABSTRACT An important facet of the Staphylococcus aureus host-pathogen interaction is the ability of the invading bacterium to evade host innate defenses, particularly the cocktail of host antimicrobial peptides. In this work, we showed that IsdA, a surface protein of S. aureus which is required for nasal colonization, binds to lactoferrin, the most abundant antistaphylococcal polypeptide in human nasal secretions. The presence of IsdA on the surface of S. aureus confers resistance to killing by lactoferrin. In addition, the bactericidal activity of lactoferrin was inhibited by addition of phe

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13

Pannkuk, Evan L., Thomas S. Risch, and Brett J. Savary. "Isolation and Identification of an Extracellular Subtilisin-Like Serine Protease Secreted by the Bat Pathogen Pseudogymnoascus destructans." PLoS ONE 10, no. 3 (2015): e0120508. https://doi.org/10.5281/zenodo.13527870.

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(Uploaded by Plazi for the Bat Literature Project) White nose syndrome (WNS) is a cutaneous fungal disease of bats. WNS is responsible for unprecedented mortalities in North American cave bat populations. There have been few descriptions of enzyme activities that may function in WNS host/pathogen interactions, while no study has isolated and described secreted proteases. To address the hypothesis that Pseudogymnoascus destructans secretes extracellular proteases that function in wing necrosis during WNS infection, the object of this study was to culture P. destructans on various media, then is

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14

Pannkuk, Evan L., Thomas S. Risch, and Brett J. Savary. "Isolation and Identification of an Extracellular Subtilisin-Like Serine Protease Secreted by the Bat Pathogen Pseudogymnoascus destructans." PLoS ONE 10, no. 3 (2015): e0120508. https://doi.org/10.5281/zenodo.13527870.

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(Uploaded by Plazi for the Bat Literature Project) White nose syndrome (WNS) is a cutaneous fungal disease of bats. WNS is responsible for unprecedented mortalities in North American cave bat populations. There have been few descriptions of enzyme activities that may function in WNS host/pathogen interactions, while no study has isolated and described secreted proteases. To address the hypothesis that Pseudogymnoascus destructans secretes extracellular proteases that function in wing necrosis during WNS infection, the object of this study was to culture P. destructans on various media, then is

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15

Pannkuk, Evan L., Thomas S. Risch, and Brett J. Savary. "Isolation and Identification of an Extracellular Subtilisin-Like Serine Protease Secreted by the Bat Pathogen Pseudogymnoascus destructans." PLoS ONE 10, no. 3 (2015): e0120508. https://doi.org/10.5281/zenodo.13527870.

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(Uploaded by Plazi for the Bat Literature Project) White nose syndrome (WNS) is a cutaneous fungal disease of bats. WNS is responsible for unprecedented mortalities in North American cave bat populations. There have been few descriptions of enzyme activities that may function in WNS host/pathogen interactions, while no study has isolated and described secreted proteases. To address the hypothesis that Pseudogymnoascus destructans secretes extracellular proteases that function in wing necrosis during WNS infection, the object of this study was to culture P. destructans on various media, then is

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16

Pannkuk, Evan L., Thomas S. Risch, and Brett J. Savary. "Isolation and Identification of an Extracellular Subtilisin-Like Serine Protease Secreted by the Bat Pathogen Pseudogymnoascus destructans." PLoS ONE 10, no. 3 (2015): e0120508. https://doi.org/10.5281/zenodo.13527870.

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(Uploaded by Plazi for the Bat Literature Project) White nose syndrome (WNS) is a cutaneous fungal disease of bats. WNS is responsible for unprecedented mortalities in North American cave bat populations. There have been few descriptions of enzyme activities that may function in WNS host/pathogen interactions, while no study has isolated and described secreted proteases. To address the hypothesis that Pseudogymnoascus destructans secretes extracellular proteases that function in wing necrosis during WNS infection, the object of this study was to culture P. destructans on various media, then is

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17

Kamekura, Masahiro, and Yukio Seno. "A halophilic extracellular protease from a halophilic archaebacterium strain 172 P1." Biochemistry and Cell Biology 68, no. 1 (1990): 352–59. http://dx.doi.org/10.1139/o90-048.

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An unidentified halophilic archaebacterium strain 172 P1 produced three extracellular proteases in media containing 15–27% salts. One component, F-II, was purified to homogeneity. It is a serine protease that can be inhibited by phenylmethylsulfonyl fluoride and chymostatin. A high concentration of NaCl was required for its stability; in the presence of 25% NaCl, only 4% of the activity was lost by incubating at 60 °C for 30 min, while complete inactivation occurred in the presence of 5% NaCl. F-II is a thermophilic and halophilic protease. High activity was obtained at 75–80 °C when F-II was

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18

Sarin, A., D. H. Adams, and P. A. Henkart. "Protease inhibitors selectively block T cell receptor-triggered programmed cell death in a murine T cell hybridoma and activated peripheral T cells." Journal of Experimental Medicine 178, no. 5 (1993): 1693–700. http://dx.doi.org/10.1084/jem.178.5.1693.

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The hypothesis that cytoplasmic proteases play a functional role in programmed cell death was tested by examining the effect of protease inhibitors on the T cell receptor-mediated death of the 2B4 murine T cell hybridoma and activated T cells. The cysteine protease inhibitors trans-epoxysuccininyl-L-leucylamido-(4-guanidino) butane (E-64) and leupeptin, the calpain selective inhibitor acetyl-leucyl-leucyl-normethional, and the serine protease inhibitors diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride, all showed dose-dependent blocking of the 2B4 death response triggered by the T

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19

Kirouac, Martin, Vincent Vachon, Delphine Quievy, Jean-Louis Schwartz, and Raynald Laprade. "Protease Inhibitors Fail To Prevent Pore Formation by the Activated Bacillus thuringiensis Toxin Cry1Aa in Insect Brush Border Membrane Vesicles." Applied and Environmental Microbiology 72, no. 1 (2006): 506–15. http://dx.doi.org/10.1128/aem.72.1.506-515.2006.

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ABSTRACT To investigate whether membrane proteases are involved in the activity of Bacillus thuringiensis insecticidal toxins, the rate of pore formation by trypsin-activated Cry1Aa was monitored in the presence of a variety of protease inhibitors with Manduca sexta midgut brush border membrane vesicles and by a light-scattering assay. Most of the inhibitors tested had no effect on the pore-forming ability of the toxin. However, phenylmethylsulfonyl fluoride, a serine protease inhibitor, promoted pore formation, although this stimulation only occurred at higher inhibitor concentrations than th

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20

Desautels, M., and R. A. Dulos. "Temperature and phenylmethylsulfonyl fluoride sensitive loss of uncoupling protein in isolated brown adipose tissue mitochondrial membranes." Biochemistry and Cell Biology 72, no. 1-2 (1994): 1–7. http://dx.doi.org/10.1139/o94-001.

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When a membrane suspension prepared from isolated rat brown fat mitochondria was incubated at 37 °C for 4 h, a loss of uncoupling protein (UCP) immunoreactivity was observed on Western blots. Analysis of [3H]GDP-binding characteristics to UCP in isolated membranes also showed a significant reduction in Bmax without significant effect on Kd. The loss of UCP was not due to protease contamination from lysosomes or mast cell granules, since loss of UCP was still observed when mitochondria were treated with digitonin to lyse lysosomes prior to membrane preparation and when mitochondria were isolate

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21

Kamada, T., C. E. Bracker, E. Lippman, and S. Bartnicki-Garcia. "Unexpected destruction of chitosomal chitin synthetase by an endogenous protease during sucrose density gradient purification." Journal of Cell Science 99, no. 3 (1991): 565–70. http://dx.doi.org/10.1242/jcs.99.3.565.

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Because of their intrinsic low buoyant density, chitosomes can be separated from crude cell homogenates (1000 g or 35,000 g supernatants) of Mucor rouxii by isopycnic sedimentation in sucrose density gradients. To accelerate and simplify the isolation of chitosomes, we centrifuged the cell-free extracts at ultrahigh speed (in a fixed-angle rotor at forces up to 311,000 g Rav) and found that the duration of centrifugation was critical. Prolonged centrifugation at ultrahigh speed caused severe distortion of the chitin synthetase profile in the gradient as the peak of chitosomal chitin synthetase

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22

Deng, Yilun, and Arco Y. Jeng. "Soluble endothelin degradation enzyme activities in various rat tissues." Biochemistry and Cell Biology 70, no. 12 (1992): 1385–89. http://dx.doi.org/10.1139/o92-187.

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From soluble extract of rat kidney we have previously identified an endothelin degradation enzyme that rapidly and specifically cleaves off the C-terminal tryptophan of endothelin-1, resulting in a peptide that is three orders of magnitude weaker in potency than endothelin-1 in causing smooh muscle contraction. The tissue distribution of this enzyme was examined, and the soluble extracts of rat kidney were found to contain the highest enzyme activity, followed by the spleen and the liver. In contrast, no enzyme activity was detected in the soluble extracts of brain, heart, and lung. The bioche

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23

Desautels, M. "ATP-stimulated protease activity in brown fat mitochondria: response to a 24-h fast in mice." Biochemistry and Cell Biology 70, no. 9 (1992): 765–69. http://dx.doi.org/10.1139/o92-116.

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Brown fat mitochondria have [3H]casein-hydrolyzing activity at pH 8.0 associated with both membrane and soluble fractions. An ATP-stimulated proteolytic activity inhibited by vanadate and N-ethylmaleimide was found in the soluble fraction. Membrane-associated proteolytic activity was inhibited by phenylmethylsulfonyl fluoride and trypsin inhibitor, suggesting that it is a serine protease. A 24-h fast in mice caused a significant loss of mitochondrial proteins from the tissue, but had no effect on protease activity of isolated mitochondria with or without ATP. The ATP-stimulated release of amin

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24

Ripert, Gabrielle, Silvia M. Racedo, Anne-Marie Elie, Claudine Jacquot, Philippe Bressollier, and Maria C. Urdaci. "Secreted Compounds of the Probiotic Bacillus clausii Strain O/C Inhibit the Cytotoxic Effects Induced by Clostridium difficile and Bacillus cereus Toxins." Antimicrobial Agents and Chemotherapy 60, no. 6 (2016): 3445–54. http://dx.doi.org/10.1128/aac.02815-15.

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Although the use of probiotics based onBacillusstrains to fight off intestinal pathogens and antibiotic-associated diarrhea is widespread, the mechanisms involved in producing their beneficial effects remain unclear. Here, we studied the ability of compounds secreted by the probioticBacillus clausiistrain O/C to counteract the cytotoxic effects induced by toxins of two pathogens,Clostridium difficileandBacillus cereus, by evaluating eukaryotic cell viability and expression of selected genes. Coincubation ofC. difficileandB. cereustoxic culture supernatants with theB. clausiisupernatant complet

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Lebuan, Urbanus Yustus, Roga Florida Kembaren, Merry Meryam Martgrita, and Cut Rizlani Kholibrina. "Thrombolytic protease characterization from leaves and fruit flesh of the jernang rattan plant (Daemonorops draco)." Indonesian Journal of Biotechnology 28, no. 4 (2023): 248. http://dx.doi.org/10.22146/ijbiotech.82390.

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Thrombolytic agents are used for thrombolytic therapy to dissolve blood clots that form in a blood vessel. All currently used thrombolytic agents have unfavorable shortcomings, such as gastrointestinal bleeding, allergic reactions, and thrombolytic agent resistance, treatment for some of which can be quite expensive. As a result, the search for thrombolytic agents derived from plants is currently taking place. Some plants have been discovered to contain protease enzymes with thrombolytic activity; pharmaceuticals derived from plants are believed to be safer. Jernang rattan (Daemonorops draco)

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Yui, Satoru, Yuuki Osawa, Takeo Ichisugi, and Riyo Morimoto-Kamata. "Neutrophil Cathepsin G, but Not Elastase, Induces Aggregation of MCF-7 Mammary Carcinoma Cells by a Protease Activity-Dependent Cell-Oriented Mechanism." Mediators of Inflammation 2014 (2014): 1–12. http://dx.doi.org/10.1155/2014/971409.

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We previously found that a neutrophil serine protease, cathepsin G, weakens adherence to culture substrates and induces E-cadherin-dependent aggregation of MCF-7 human breast cancer cells through its protease activity. In this study, we examined whether aggregation is caused by degradation of adhesion molecules on the culture substrates or through an unidentified mechanism. We compared the effect of treatment with cathepsin G and other proteases, including neutrophil elastase against fibronectin- (FN-) coated substrates. Cathepsin G and elastase potently degraded FN on the substrates and induc

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27

Shimamoto, Seiko, Ryuichi Moriyama, Kazuhiro Sugimoto, Shigeru Miyata, and Shio Makino. "Partial Characterization of an Enzyme Fraction with Protease Activity Which Converts the Spore Peptidoglycan Hydrolase (SleC) Precursor to an Active Enzyme during Germination of Clostridium perfringens S40 Spores and Analysis of a Gene Cluster Involved in the Activity." Journal of Bacteriology 183, no. 12 (2001): 3742–51. http://dx.doi.org/10.1128/jb.183.12.3742-3751.2001.

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ABSTRACT A spore cortex-lytic enzyme of Clostridium perfringensS40 which is encoded by sleC is synthesized at an early stage of sporulation as a precursor consisting of four domains. After cleavage of an N-terminal presequence and a C-terminal prosequence during spore maturation, inactive proenzyme is converted to active enzyme by processing of an N-terminal prosequence with germination-specific protease (GSP) during germination. The present study was undertaken to characterize GSP. In the presence of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid (CHAPS), a nondenaturing deterg

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Valenzuela, Bernardita, Francisco Solís-Cornejo, Rubén Araya, and Pedro Zamorano. "Isolation and Characterization of Thermus thermophilus Strain ET-1: An Extremely Thermophilic Bacterium with Extracellular Thermostable Proteolytic Activity Isolated from El Tatio Geothermal Field, Antofagasta, Chile." International Journal of Molecular Sciences 24, no. 19 (2023): 14512. http://dx.doi.org/10.3390/ijms241914512.

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The present study describes the isolation of an extremely thermophilic bacterium from El Tatio, a geyser field in the high planes of Northern Chile. The thermophile bacterium named Thermus thermophilus strain ET-1 showed 99% identity with T. thermophilus SGO.5JP 17-16 (GenBank accession No. CP002777) by 16S rDNA gene analysis. Morphologically, the cells were non-sporeforming Gram-negative rods that formed colonies with yellow pigmentation. This strain is able to proliferate between 55 and 80 °C with a pH range of 6–10, presenting an optimum growth rate at 80 °C and pH 8. The bacterium produces

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Afoshin, Alexey S., Mihail A. Konstantinov, Ilya Yu Toropygin, Irina V. Kudryakova та Natalia V. Vasilyeva. "β-Lytic Protease of Lysobacter capsici VKM B-2533T". Antibiotics 9, № 11 (2020): 744. http://dx.doi.org/10.3390/antibiotics9110744.

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Bacteriolytic enzymes are promising antimicrobial agents for developing new-generation drugs. Recently, we have isolated a β-lytic protease (BlpLc) from the culture liquid of Lysobacter capsici VKM B-2533T. This BlpLc possesses a valuable property, not described for β-lytic proteases (Blps) earlier, of hydrolyzing living cells of Staphylococcus aureus 55 MRSA clinical isolate. This work phylogenetically characterized the BlpLc and investigated its properties. Analysis revealed a variability of pre-/pro-parts of Blp precursors. The mature BlpLc is the closest to the earlier annotated but not is

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Juarez, Zaira E., and Murray W. Stinson. "An Extracellular Protease of Streptococcus gordonii Hydrolyzes Type IV Collagen and Collagen Analogues." Infection and Immunity 67, no. 1 (1999): 271–78. http://dx.doi.org/10.1128/iai.67.1.271-278.1999.

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ABSTRACT Streptococcus gordonii is a frequent cause of infective bacterial endocarditis, but its mechanisms of virulence are not well defined. In this study, streptococcal proteases were recovered from spent chemically defined medium (CDM) and fractionated by ammonium sulfate precipitation and by ion-exchange and gel filtration column chromatography. Three proteases were distinguished by their different solubilities in ammonium sulfate and their specificities for synthetic peptides. One of the enzymes cleaved collagen analogs Gly-Pro 4-methoxy-β-naphthylamide, 2-furanacryloyl-Leu-Gly-Pro-Ala (

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31

Kusuma, C. G., Vinod Gubbiveeranna, C. K. Sumachirayu, S. Bhavana, H. Ravikumar, and S. Nagaraju. "The hemostatic activity of Manilkara zapota (L.) P. Royen latex associated with fibrinogenolytic activity." Plant Science Today 7, no. 3 (2020): 469–75. http://dx.doi.org/10.14719/pst.2020.7.3.775.

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Manilkara zapota (L.) P. Royen (Sapotaceae), is widely used in traditional medicine for various ailments like, diarrhea, pulmonary diseases, piles, ulcers and to treat wounds. The present study evaluates the role of M. zapota latex in hemostasis. The processed latex named as M. zapota natant latex (MzNL), has proteins at the concentration of 8 mg/ml and showed protein bands in Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The proteolytic activity of MzNL was evaluated using casein in comparison with trypsin. The phenylmethylsulfonyl fluoride (PMSF) inhibited the proteas

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Zaman, Md Asad Uz, Taqiyah Akhtar, ATM Zafrul Azam, Md Arafat Al Mamun, Md Mozammel Hoq, and Md Abdul Mazid. "Thrombolytic Activity of Alkaline Protease Purified from a Mutant Strain Bacillus licheniformis MZK05M9." Bangladesh Pharmaceutical Journal 21, no. 1 (2018): 63–70. http://dx.doi.org/10.3329/bpj.v21i1.37908.

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Investigations were performed to find out new microbial enzymes as thrombolytics having better efficacy and specificity. Mutant strain of Bacillus species, B. licheniformis MZK05M9 was cultured in modified urea-glucose media followed by purification using ammonium sulphate precipitation and ultrafiltration through centricon tube of specific MWCO value. The production method yielded 823.42 units/mg of the crude enzyme from mutant strain MZK05M9 and after purification 37695.64 units/mg. The molecular weight of the purified enzyme was estimated as 27.2 kDa and purification increased its specific

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Henke, Markus O., Gerrit John, Christina Rheineck, Shashi Chillappagari, Lutz Naehrlich, and Bruce K. Rubin. "Serine Proteases Degrade Airway Mucins in Cystic Fibrosis." Infection and Immunity 79, no. 8 (2011): 3438–44. http://dx.doi.org/10.1128/iai.01252-10.

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ABSTRACTAirway mucins are the major molecular constituents of mucus. Mucus forms the first barrier to invading organisms in the airways and is an important defense mechanism of the lung. We confirm that mucin concentrations are significantly decreased in airway secretions of subjects with cystic fibrosis (CF) who have chronicPseudomonas aeruginosainfection. In sputum from CF subjects without a history ofP. aeruginosa, we found no significant difference in the mucin concentration compared to mucus from normal controls. We demonstrate that mucins can be degraded by synthetic human neutrophil ela

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Fakhfakh, Nahed, Safia Kanoun, Laila Manni, and Moncef Nasri. "Production and biochemical and molecular characterization of a keratinolytic serine protease from chicken feather-degradingBacillus licheniformisRPk." Canadian Journal of Microbiology 55, no. 4 (2009): 427–36. http://dx.doi.org/10.1139/w08-143.

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A novel feather-degrading bacterium was isolated from a polluted river and identified as Bacillus licheniformis RPk. The isolate exhibited high proteinase production when grown in chicken-feather media. Complete feather degradation was achieved during cultivation. Maximum protease activity (4150 U/mL with casein as a substrate and 37.35 U/mL with keratin as a substrate) was obtained when the strain was grown in a medium containing 7.5 g/L chicken feathers, 2 g/L yeast extract, 0.5 g/L NaCl, 0.1 g/L MgSO4·7H2O, 0.7 g/L KH2PO4, and 1.4 g/L K2HPO4for 48 h with agitation of 200 rev/min at 37 °C. T

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Fournier, Bénédicte, and David C. Hooper. "A New Two-Component Regulatory System Involved in Adhesion, Autolysis, and Extracellular Proteolytic Activity ofStaphylococcus aureus." Journal of Bacteriology 182, no. 14 (2000): 3955–64. http://dx.doi.org/10.1128/jb.182.14.3955-3964.2000.

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ABSTRACT A transposition mutant of Staphylococcus aureus was selected from the parent strain MT23142, a derivative of strain 8325. The site of transposition was near the 5′ terminus of the genearlS. ArlS exhibits strong similarities with histidine protein kinases. Sequence analysis suggested that arlSforms an operon with upstream gene arlR. The predicted product of arlR is a member of the OmpR-PhoB family of response regulators. The arlS mutant formed a biofilm on a polystyrene surface unlike the parent strain and the complemented mutant. Biofilm formation was associated with increased primary

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Zaman, Md Asad Uz, Md Arafat Al Mamun, Shakila Nargis Khan, Md Mozammel Hoq, and Md Abdul Mazid. "Partial Purification of Alkaline Protease as Thrombolytic Agent from Mutant Strain Bacillus licheniformis EMS250-O-1." Dhaka University Journal of Pharmaceutical Sciences 15, no. 2 (2017): 135–41. http://dx.doi.org/10.3329/dujps.v15i2.30926.

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Thrombosis leads to myocardial infarction, stroke and other cardiovascular complications. Microbial thrombolytic agents such as urokinase, streptokinase etc. are used to treat complications related to thrombosis. To search for new microbial enzymes as thrombolytics having better efficacy and specificity, Bacillus licheniformis EMS-O-1 mutant strain was cultured in modified urea-molasses media followed by purification using ammonium sulphate precipitation and ultrafiltration through centricon tube of 100 MWCO value. The yield of crude enzyme was 11129.14 U/mg and after purification 40180.46 U/m

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SILVA, Germana Michelle de Medeiros e., Raquel Pedrosa BEZERRA, José António TEIXEIRA, et al. "Screening, production and biochemical characterization of a new fibrinolytic enzyme produced by Streptomyces sp. (Streptomycetaceae) isolated from Amazonian lichens." Acta Amazonica 46, no. 3 (2016): 323–32. http://dx.doi.org/10.1590/1809-4392201600022.

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ABSTRACT Thrombosis is a pathophysiological disorder caused by accumulation of fibrin in the blood. Fibrinolytic proteases with potent thrombolytic activity have been produced by diverse microbial sources. Considering the microbial biodiversity of the Amazon region, this study aimed at the screening, production and biochemical characterization of a fibrinolytic enzyme produced by Streptomyces sp. isolated from Amazonian lichens. The strain Streptomyces DPUA1576 showed the highest fibrinolytic activity, which was 283 mm2. Three variables at two levels were used to assess their effects on the fi

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Villaseca, Jorge M., Fernando Navarro-García, Guillermo Mendoza-Hernández, James P. Nataro, Alejandro Cravioto, and Carlos Eslava. "Pet Toxin from Enteroaggregative Escherichia coli Produces Cellular Damage Associated with Fodrin Disruption." Infection and Immunity 68, no. 10 (2000): 5920–27. http://dx.doi.org/10.1128/iai.68.10.5920-5927.2000.

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ABSTRACT Pet toxin is a serine protease from enteroaggregativeEscherichia coli which has been described as causing enterotoxic and cytotoxic effects. In this paper we show that Pet produces spectrin and fodrin (nonerythroid spectrin) disruption. Using purified erythrocyte membranes treated with Pet toxin, we observed degradation of α- and β-spectrin chains; this effect was dose and time dependent, and a 120-kDa protein fraction was observed as a breakdown product. Spectrin degradation and production of the 120-kDa subproduct were confirmed using specific antibodies against the α- and β-spectri

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Navarro-García, Fernando, Cynthia Sears, Carlos Eslava, Alejandro Cravioto, and James P. Nataro. "Cytoskeletal Effects Induced by Pet, the Serine Protease Enterotoxin of Enteroaggregative Escherichia coli." Infection and Immunity 67, no. 5 (1999): 2184–92. http://dx.doi.org/10.1128/iai.67.5.2184-2192.1999.

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ABSTRACT We have previously described enteroaggregative Escherichia coli (EAEC) strains that induce cytotoxic effects on T84 cells, ligated rat ileal loops, and human intestine in culture. Such strains secrete a 104-kDa protein termed Pet (for plasmid-encoded toxin). We have also shown previously that the Pet toxin induces rises in short-circuit current and decreases the electrical resistance in rat jejunum mounted in an Ussing chamber. The nucleotide sequence of thepet gene revealed that Pet is a member of the autotransporter class of secreted proteins. Here we show that a concentrated supern

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Lee, Sun-og, Junichi Kato, Noboru Takiguchi, et al. "Involvement of an Extracellular Protease in Algicidal Activity of the Marine Bacterium Pseudoalteromonassp. Strain A28." Applied and Environmental Microbiology 66, no. 10 (2000): 4334–39. http://dx.doi.org/10.1128/aem.66.10.4334-4339.2000.

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ABSTRACT The marine bacterium Pseudoalteromonas sp. strain A28 was able to kill the diatom Skeletonema costatum strain NIES-324. The culture supernatant of strain A28 showed potent algicidal activity when it was applied to a paper disk placed on a lawn ofS. costatum NIES-324. The condensed supernatant, which was prepared by subjecting the A28 culture supernatant to ultrafiltration with a 10,000-M w-cutoff membrane, showed algicidal activity, suggesting that strain A28 produced extracellular substances capable of killing S. costatum cells. The condensed supernatant was then found to have protea

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El Ouriaghli, Frank, Hiroshi Fujiwara, J. Joseph Melenhorst, Giuseppe Sconocchia, Nancy Hensel, and A. John Barrett. "Neutrophil elastase enzymatically antagonizes the in vitro action of G-CSF: implications for the regulation of granulopoiesis." Blood 101, no. 5 (2003): 1752–58. http://dx.doi.org/10.1182/blood-2002-06-1734.

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There is evidence that neutrophil production is a balance between the proliferative action of granulocyte–colony-stimulating factor (G-CSF) and a negative feedback from mature neutrophils (the chalone). Two neutrophil serine proteases have been implicated in granulopoietic regulation: pro–proteinase 3 inhibits granulocyte macrophage–colony-forming unit (CFU-GM) growth, and elastase mutations cause cyclic and congenital neutropenia. We further studied the action of the neutrophil serine proteases (proteinase 3, elastase, azurocidin, and cathepsin G) on granulopoiesis in vitro. Elastase inhibite

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Kyriachenko, Y., O. Oskyrko, I. Udovychenko, and T. Halenova. "Hemolytic activity of skin secretions of amphibians that inhabit the Ukraine territory." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 80, no. 1 (2020): 6–10. http://dx.doi.org/10.17721/1728_2748.2020.80.6-10.

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Secretions derived from amphibian skin glands serve as a potential reservoir of various valuable active molecules. Currently, the multiple substances with diverse therapeutic activities among the components of glandular secretions of different species of amphibians have been found. It has been proven that they have antibacterial, antifungal, antiprotozoal, antidiabetic, antineoplastic, analgesic, and sleep-inducing properties. Taking this into consideration, to get the basic knowledge about the properties of the components of skin secretions of some Anura species that inhabit the territory of

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Leher, Henry, Robert Silvany, Hassan Alizadeh, Jing Huang, and Jerry Y. Niederkorn. "Mannose Induces the Release of Cytopathic Factors from Acanthamoeba castellanii." Infection and Immunity 66, no. 1 (1998): 5–10. http://dx.doi.org/10.1128/iai.66.1.5-10.1998.

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ABSTRACT Acanthamoeba keratitis is a chronic inflammatory disease of the cornea which is highly resistant to many antimicrobial agents. The pathogenic mechanisms of this disease are poorly understood. However, it is believed that the initial phases in the pathogenesis of Acanthamoeba keratitis involve parasite binding and lysis of the corneal epithelium. These processes were examined in vitro, usingAcanthamoeba castellanii trophozoites. Parasites readily adhered to Chinese hamster corneal epithelial cells in vitro; however, parasite binding was strongly inhibited by mannose but not by lactose.

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Raksha, Nataliia, Oleksandr Maievskyi, Iryna Dzevulska, et al. "PROTEOLYTIC ACTIVITY IN THE HEART OF RATS WITH HYPERHOMOCYSTEINEMIA." Wiadomości Lekarskie 75, no. 4 (2022): 831–35. http://dx.doi.org/10.36740/wlek202204115.

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The aim: To investigate the distribution of proteolytic activity and cytokine profile in the heart of rats with hyperhomocysteinemia. Materials and methods: A total of 60 albino non-linear male rats was used in the study. Hyperhomocysteinemia was induced by intragastric administration of DL-homocysteine thiolactone. Total proteolytic activity was measured using casein as a substrate. To determine the activity of metal-dependent and serine proteases, ethylenediaminetetraacetic acid, and phenylmethylsulfonyl fluoride were used. The level of matrix metalloproteinases, tissue inhibitor of metallop

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Mignino, Lorena A., Marcos Crupkin, and María E. Paredi. "Proteolytic Activity in Actomyosinfrom Mantle and Fin of Squid (Illexargentinus) Stored at 2-4°C. Influence on the Physicochemical and Functional Properties of the Protein." Journal of Food Research 2, no. 2 (2013): 55. http://dx.doi.org/10.5539/jfr.v2n2p55.

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<p>Actomyosin (AM) of mantle and fin from squid was stored at 2-4°C and the possible presence of proteolytic activity was investigated. Similar SDS-PAGE 10% patterns were obtained with both AM at zero time of storage. In absence of protease inhibitors, a decrease in the intensity of the band of the myosin heavy chain (MHC) and an increase in those of 155 kDa and 55 kDa bands of stored AM was observed. In presence of either PMSF (phenylmethylsulfonyl fluoride) or EDTA (ethylenediaminetetraacetic acid) both AM showed a minor degradation of the MHC, being the EDTA more effective. Proteolyti

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Saha, Kreesha, and Clark Gedney. "Impact of PETase’s Active Site Disulfide Bond on PET Biodegradation." Fine Focus 8, no. 1 (2022): 86–99. http://dx.doi.org/10.33043/ff.8.1.86-99.

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Plastic pollution is one of the largest problems globally, with polyethylene terephthalate (PET) plastic as one of the main sources. Effective depolymerization of PET to its monomers for upcycling is a challenge. PETase is reported to be an effective enzyme for biodegradation of PET via C-O bond cleavage of ester linkage. The role of the disulfide bond, present in PETase’s active site sequence, is unknown in the cleavage of PET’s ester linkage. To understand the role of this bond, two separate versions of PETase – one containing the disulfide bond, and the other without the disulfide bond - we

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Atasoy, Fikriye, та Naşit İğci. "Determination of the fibrinogenolytic activity of Montivipera raddei (Raddeʼs mountain viper) venom". Archives of Biological Sciences, № 00 (2022): 29. http://dx.doi.org/10.2298/abs220806029a.

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Snake venom fibrinogenolytic enzymes have diagnostic and therapeutic value and are important for snakebite pathology. In the present study, the fibrinogenolytic activity of Montivipera raddei venom was investigated. Crude venom was incubated with human fibrinogen for different times at 37?C. An inhibition study was carried out using different protease inhibitors. The fibrinogenolytic activity was assessed by SDS-PAGE and fibrinogen zymography. An HPLC-based method was used to obtain confirmatory data. Montivipera raddei venom predominantly cleaved the A? chain of fibrinogen in a time-dependent

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Pepperell, John R., Gabor Nemeth, Yuji Yamada, Frederick Naftolin, and Maricruz Merino. "Localized accumulation of angiotensin II and production of angiotensin-(1–7) in rat luteal cells and effects on steroidogenesis." American Journal of Physiology-Endocrinology and Metabolism 291, no. 2 (2006): E221—E233. http://dx.doi.org/10.1152/ajpendo.00205.2005.

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These studies aim to investigate subcellular distribution of angiotensin II (ANG II) in rat luteal cells, identify other bioactive angiotensin peptides, and investigate a role for angiotensin peptides in luteal steroidogenesis. Confocal microscopy showed ANG II distributed within the cytoplasm and nuclei of luteal cells. HPLC analysis showed peaks that eluted with the same retention times as ANG-(1–7), ANG II, and ANG III. Their relative concentrations were ANG II ≥ ANG-(1–7) > ANG III, and accumulation was modulated by quinapril, an inhibitor of angiotensin-converting enzyme (ACE), Z-propr

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Martínez, Virginia, Fernando de la Peña, Javier García-Hidalgo, Isabel de la Mata, José Luis García, and María Auxiliadora Prieto. "Identification and Biochemical Evidence of a Medium-Chain-Length Polyhydroxyalkanoate Depolymerase in the Bdellovibrio bacteriovorus Predatory Hydrolytic Arsenal." Applied and Environmental Microbiology 78, no. 17 (2012): 6017–26. http://dx.doi.org/10.1128/aem.01099-12.

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ABSTRACTThe obligate predatorBdellovibrio bacteriovorusHD100 shows a large set of proteases and other hydrolases as part of its hydrolytic arsenal needed for its predatory life cycle. We present genetic and biochemical evidence that open reading frame (ORF) Bd3709 ofB. bacteriovorusHD100 encodes a novel medium-chain-length polyhydroxyalkanoate (mcl-PHA) depolymerase (PhaZBd). The primary structure of PhaZBdsuggests that this enzyme belongs to the α/β-hydrolase fold family and has a typical serine hydrolase catalytic triad (serine-histidine-aspartic acid) in agreement with other PHA depolymeras

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Bamford, Caroline V., J. Christopher Fenno, Howard F. Jenkinson, and David Dymock. "The Chymotrypsin-Like Protease Complex of Treponema denticola ATCC 35405 Mediates Fibrinogen Adherence and Degradation." Infection and Immunity 75, no. 9 (2007): 4364–72. http://dx.doi.org/10.1128/iai.00258-07.

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ABSTRACT Treponema denticola is an anaerobic spirochete strongly associated with human periodontal disease. T. denticola bacteria interact with a range of host tissue proteins, including fibronectin, laminin, and fibrinogen. The latter localizes in the extracellular matrix where tissue damage has occurred, and interactions with fibrinogen may play a key role in T. denticola colonization of the damaged sites. T. denticola ATCC 35405 showed saturable binding of fluid-phase fibrinogen to the cell surface and saturable adherence to immobilized fibrinogen. Levels of fibrinogen binding were enhanced

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