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1

Amri, Muhammad Faiz, Edi Husen, Aris Tjahjoleksono, and Aris Tri Wahyudi. "Alkaline Phosphatase Activity of Plant Growth-Promoting Actinomycetes and Their Genetic Diversity Based on the phoD Gene." HAYATI Journal of Biosciences 29, no. 3 (2022): 360–69. http://dx.doi.org/10.4308/hjb.29.3.360-369.

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Actinomycete is one of the beneficial bacteria groups inhabiting rhizosphere soil. They can promote plant growth through various mechanisms. In the previous study we have isolated rhizosphere actinomycetes from maize rhizosphere with direct plant growth promotion characters. The aims of the present study were to analyze the ability of maize rhizosphere actinomycetes to solubilize phosphate, determine alkaline phosphatase activity, and study their genetic diversity based on phoD gene. Thirteen rhizosphere actinomycete isolates were able to solubilize phosphate at concertation range 55.84±2.27 m
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2

Balabanova, Larissa, Svetlana Bakholdina, Nina Buinovskaya, et al. "LPS-Dephosphorylating Cobetia amphilecti Alkaline Phosphatase of PhoA Family Divergent from the Multiple Homologues of Cobetia spp." Microorganisms 12, no. 3 (2024): 631. http://dx.doi.org/10.3390/microorganisms12030631.

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A highly active alkaline phosphatase (ALP) of the protein structural family PhoA, from a mussel gut-associated strain of the marine bacterium Cobetia amphilecti KMM 296 (CmAP), was found to effectively dephosphorylate lipopolysaccharides (LPS). Therefore, the aim of this work was to perform a comprehensive bioinformatics analysis of the structure, and to suggest the physiological role of this enzyme in marine bacteria of the genus Cobetia. A scrutiny of the CmAP-like sequences in 36 available Cobetia genomes revealed nine homologues intrinsic to the subspecies C. amphilecti, whereas PhoA of a
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3

Antelmann, Haike, Christian Scharf, and Michael Hecker. "Phosphate Starvation-Inducible Proteins ofBacillus subtilis: Proteomics and Transcriptional Analysis." Journal of Bacteriology 182, no. 16 (2000): 4478–90. http://dx.doi.org/10.1128/jb.182.16.4478-4490.2000.

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ABSTRACT The phosphate starvation response in Bacillus subtiliswas analyzed using two-dimensional (2D) polyacrylamide gel electrophoresis of cell extracts and supernatants from phosphate-starved cells. Most of the phosphate starvation-induced proteins are under the control of ςB, the activity of which is increased by energy depletion. In order to define the proteins belonging to the Pho regulon, which is regulated by the two-component regulatory proteins PhoP and PhoR, the 2D protein pattern of the wild type was compared with those of a sigB mutant and aphoR mutant. By matrix-assisted laser de
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4

Kageyama, Hakuto, Keshawanand Tripathi, Ashwani K. Rai, Suriyan Cha-um, Rungaroon Waditee-Sirisattha, and Teruhiro Takabe. "An Alkaline Phosphatase/Phosphodiesterase, PhoD, Induced by Salt Stress and Secreted Out of the Cells of Aphanothece halophytica, a Halotolerant Cyanobacterium." Applied and Environmental Microbiology 77, no. 15 (2011): 5178–83. http://dx.doi.org/10.1128/aem.00667-11.

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ABSTRACTAlkaline phosphatases (APases) are important enzymes in organophosphate utilization. Three prokaryotic APase gene families, PhoA, PhoX, and PhoD, are known; however, their functional characterization in cyanobacteria largely remains to be clarified. In this study, we cloned thephoDgene from a halotolerant cyanobacterium,Aphanothece halophytica(phoDAp). The deduced protein, PhoDAp, contains Tat consensus motifs and a peptidase cleavage site at the N terminus. The PhoDApenzyme was activated by Ca2+and exhibited APase and phosphodiesterase (APDase) activities. Subcellular localization exp
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5

Neha, Neha, Yashpal Bhardwaj, Bhaskar Reddy, and Suresh Kumar Dubey. "Organic Farming Favors phoD-Harboring Rhizospheric Bacterial Community and Alkaline Phosphatase Activity in Tropical Agroecosystem." Plants 12, no. 5 (2023): 1068. http://dx.doi.org/10.3390/plants12051068.

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The bacteria harboring phoD encodes alkaline phosphatase (ALP), a secretory enzyme that hydrolyzes organic phosphorous (P) to a usable form in the soil. The impact of farming practices and crop types on phoD bacterial abundance and diversity in tropical agroecosystems is largely unknown. In this research, the aim was to study the effect of farming practices (organic vs. conventional) and crop types on the phoD-harboring bacterial community. A high-throughput amplicon (phoD gene) sequencing method was employed for the assessment of bacterial diversity and qPCR for phoD gene abundance. Outcomes
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6

Wang, Fei, Ying Zhang, Yong Xia, Zhenbo Cui, and Chengyou Cao. "Soil Microbial Community Succession Based on PhoD and Gcd Genes along a Chronosequence of Sand-Fixation Forest." Forests 12, no. 12 (2021): 1707. http://dx.doi.org/10.3390/f12121707.

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Revegetation by planting shrubs on moving sand dunes is widely used to control desertification in arid/semi-arid areas. The soil including microbial community can gradually be improved along with plantation development. The purposes of this study were (1) to investigate the responses of microbial communities involved in the mineralization of soil organic phosphorus (OP) and dissolution of inorganic P (IOP) in the development of sand-fixating plantation and (2) to discuss the interactions between P turnover microbial communities and soil properties. We assessed the compositions of soil phoD gen
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7

Zhong, Feng, Naling Bai, Xiangqian Chu, Yu He, Hanlin Zhang, and Haibo Li. "Effects of Lake Sediment on Soil Properties, Crop Growth, and the phoD-Harboring Microbial Community." Agriculture 12, no. 12 (2022): 2065. http://dx.doi.org/10.3390/agriculture12122065.

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Removal of lake sediment has been shown to be an effective method for lake restoration. High phosphorus (P) content makes it possible for lake sediment to provide fertility for agricultural production. However, little research has focused on the responses of the soil-phosphorus-related microbial community to the sediment-derived fertilizer enriched in phosphorus content. The phoD-harboring gene, important to the global phosphorus cycle, encodes alkaline phosphatase hydrolyzing organic P in soil. Accordingly, a plot experiment was performed to compare the effects of four different fertilization
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8

Yu, Li, Ying Zhang, Zhenbo Cui, and Chengyou Cao. "Responses of Soil Microbial Communities Associated with Phosphorus Transformation to Land-Use Alternations in a Meadow Grassland, Northeast China." Microorganisms 13, no. 3 (2025): 624. https://doi.org/10.3390/microorganisms13030624.

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Land-use changes in meadow grasslands in semi-arid areas usually significantly affect soil environment and microbiota. However, studies on the response of soil P-cycle-related microbial communities to land-use conversions are still limited. In this study, a series of land-use types including upland field, paddy field, poplar plantation, and their adjacent natural meadow grassland in the Horqin Sandy Land of Northeast China were selected, and the diversities and structures of soil microbial communities involved in organic P mineralization (phoD-harboring community) and inorganic phosphate solub
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9

Zhou, Yanwen, Tingxi Zhang, Shengyan Jin, Siyu Chen, and Yinlong Zhang. "Effects of Escherichia coli Alkaline Phosphatase PhoA on the Mineralization of Dissolved Organic Phosphorus." Water 13, no. 23 (2021): 3315. http://dx.doi.org/10.3390/w13233315.

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Alkaline phosphatases, which play the key role in the mineralization of organic phosphorus, have been grouped into three distinct families, PhoA, PhoX, and PhoD. PhoA is still an important component of the Pho regulon for many microbes although its distribution is not as wide as that of PhoX and PhoD. However, several questions remain unclear about the effect of PhoA mineralization of dissolved organic phosphorus. In this study, the role of Escherichia coli alkaline phosphatase PhoA (hereinafter referred to as PhoA) in the mineralization of different organic phosphorus including phosphate mono
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10

Schoebitz, Mauricio, Dalma Castillo, Milko Jorquera, and Antonio Roldan. "Responses of Microbiological Soil Properties to Intercropping at Different Planting Densities in an Acidic Andisol." Agronomy 10, no. 6 (2020): 781. http://dx.doi.org/10.3390/agronomy10060781.

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Intercropping could increase the capacity of crops to use soil resources. The aim of this study was to investigate the effects of lupin/wheat intercropping on soil properties, grain yield and nutrient uptake at different plant densities. Lupin and wheat were grown under field conditions as monocrops and intercrops. Soil nutrient availability and relative abundance of functional genes (acdS, phoD, phoC and nifH) were evaluated. The results obtained indicate that the cropping system had a significant effect (p < 0.001) on N and P availability. Lupin monocrop led to significantly higher N avai
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11

Apel, Alexander K., Alberto Sola-Landa, Antonio Rodríguez-García, and Juan F. Martín. "Phosphate control of phoA, phoC and phoD gene expression in Streptomyces coelicolor reveals significant differences in binding of PhoP to their promoter regions." Microbiology 153, no. 10 (2007): 3527–37. http://dx.doi.org/10.1099/mic.0.2007/007070-0.

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12

Lu, Peng, Yamei Zhang, Bingjie Ji, et al. "PhoD Harboring Microbial Community and Alkaline Phosphatase as Affected by Long Term Fertilization Regimes on a Calcareous Soil." Agronomy 13, no. 2 (2023): 363. http://dx.doi.org/10.3390/agronomy13020363.

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Organic phosphorus (Po) may play a vital role in phosphorus availability via its mineralization by alkaline phosphatase (ALP), being encoded by phoD gene, in calcareous soil. Understanding the effects of long-term fertilization on the community of phoD harboring bacteria and the related alteration of the P availability owing to the changes in ALP secretion may offer a chance to elucidate the Po contribution to soil available P. Based on a long-term experiment, we analyzed the phoD gene harboring microbial diversity, abundance and composition, ALP and Po forms, and their relationship. The treat
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13

Chen, Min, Hanlian Qin, Yueming Liang, et al. "The phoD-Harboring Microorganism Communities and Networks in Karst and Non-Karst Forests in Southwest China." Forests 15, no. 2 (2024): 341. http://dx.doi.org/10.3390/f15020341.

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Phosphorous (P) limitation is common not only in tropical rainforest and savanna ecosystems, but also in karst forest ecosystems. Soil phoD-harboring microorganisms are essential in soil P cycles, but very little information is available about them in karst ecosystems. A total of 36 soil samples were collected from two types of forest ecosystems (karst and non-karst) over two seasons (rainy and dry), and the diversity and community structure of soil phoD-harboring microorganisms were measured. The contents of available P (AP), soil total P (TP), microbial biomass P (MBP) and the activity of al
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14

Zhang, Yang, Caidi Yang, Jun Wang, and Shenggao Lu. "Biochar Co-Applied with Lime Enhances Soil Phosphorus Availability via Microbial and Enzymatic Modulation of Paddy Soil." Microorganisms 13, no. 3 (2025): 582. https://doi.org/10.3390/microorganisms13030582.

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Soil microorganisms play a crucial role in improving soil phosphorus (P) availability. However, few studies have explored the changes in microbial community structure and their underlying mechanisms for improving soil P availability with the application of biochar and lime. Three kinds of biochar, made from rice straw (SB), Chinese fir wood sawdust (WB), and pig manure (MB), alone and with lime (SBL, WBL, and MBL), were applied to paddy soil to reveal the biochemical mechanisms for enhancing soil P availability. High-throughput sequencing and real-time PCR were used to investigate soil microbi
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15

Vogel, K., W. Hörz, and A. Hinnen. "The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions." Molecular and Cellular Biology 9, no. 5 (1989): 2050–57. http://dx.doi.org/10.1128/mcb.9.5.2050-2057.1989.

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The repressible acid phosphatase gene PHO5 of Saccharomyces cerevisiae requires the two positively acting regulatory proteins PHO2 and PHO4 for expression. pho2 or pho4 mutants are not able to derepress the PHO5 gene under low-Pi conditions. Here we show that both PHO2 and PHO4 bind specifically to the PHO5 promoter in vitro. Gel retardation assays using promoter deletions revealed two regions involved in PHO4 binding. Further characterization by DNase I footprinting showed two protected areas, one located at -347 to -373 (relative to the ATG initiator codon) (UASp1) and the other located at -
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16

Vogel, K., W. Hörz, and A. Hinnen. "The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions." Molecular and Cellular Biology 9, no. 5 (1989): 2050–57. http://dx.doi.org/10.1128/mcb.9.5.2050.

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The repressible acid phosphatase gene PHO5 of Saccharomyces cerevisiae requires the two positively acting regulatory proteins PHO2 and PHO4 for expression. pho2 or pho4 mutants are not able to derepress the PHO5 gene under low-Pi conditions. Here we show that both PHO2 and PHO4 bind specifically to the PHO5 promoter in vitro. Gel retardation assays using promoter deletions revealed two regions involved in PHO4 binding. Further characterization by DNase I footprinting showed two protected areas, one located at -347 to -373 (relative to the ATG initiator codon) (UASp1) and the other located at -
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17

Adkins, Melissa W., Stephanie K. Williams, Jeffrey Linger, and Jessica K. Tyler. "Chromatin Disassembly from the PHO5 Promoter Is Essential for the Recruitment of the General Transcription Machinery and Coactivators." Molecular and Cellular Biology 27, no. 18 (2007): 6372–82. http://dx.doi.org/10.1128/mcb.00981-07.

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ABSTRACT The disassembly of promoter nucleosomes appears to be a general property of highly transcribed eukaryotic genes. We have previously shown that the disassembly of chromatin from the promoters of the Saccharomyces cerevisiae PHO5 and PHO8 genes, mediated by the histone chaperone anti-silencing function 1 (Asf1), is essential for transcriptional activation upon phosphate depletion. This mechanism of transcriptional regulation is shared with the ADY2 and ADH2 genes upon glucose removal. Promoter chromatin disassembly by Asf1 is required for recruitment of TBP and RNA polymerase II, but no
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18

Monds, Russell D., Peter D. Newell, Julia A. Schwartzman, and George A. O'Toole. "Conservation of the Pho regulon in Pseudomonas fluorescens Pf0-1." Applied and Environmental Microbiology 72, no. 3 (2006): 1910–24. http://dx.doi.org/10.1128/aem.72.3.1910-1924.2006.

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ABSTRACT The Pho regulon integrates the sensing of environmental inorganic phosphate (Pi) availability with coregulation of gene expression, mediating an adaptive response to Pi limitation. Many aspects of the Pho regulon have been addressed in studies of Escherichia coli; however, it is unclear how transferable this knowledge is to other bacterial systems. Here, we report work to discern the conservation of the Pho regulon in Pseudomonas fluorescens Pf0-1. We demonstrate by mutational studies that PhoB/PhoR and the Pst system have conserved functions in the regulation of Pi-induced phosphatas
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19

Yu, Xuan, Lulu Feng, Yuan Huang, et al. "Planted Citrus Regulates the Community and Networks of phoD-Harboring Bacteria to Drive Phosphorus Availability Between Karst and Non-Karst Soils." Microorganisms 12, no. 12 (2024): 2582. https://doi.org/10.3390/microorganisms12122582.

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The phosphorus (P) availability in soils is influenced by microbes, particularly those containing the gene responsible for phosphate solubilization. The present study investigated the community structure, diversity, and co-occurrence networks of phoD-harboring bacteria in karst and non-karst citrus orchard soils across a planting duration gradient, natural forests, and abandoned land, as well as the soil total P (TP) and available P (AP) contents and enzyme activities. The soil AP contents were lower in the karst regions than in the non-karst regions, while the soil organic carbon (C; SOC), ex
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20

Zhang, Ying, Zhenbo Cui, and Chengyou Cao. "Effects of Secondary Salinization on Soil Phosphorus Fractions and Microbial Communities Related to Phosphorus Transformation in a Meadow Grassland, Northeast China." Agronomy 15, no. 4 (2025): 960. https://doi.org/10.3390/agronomy15040960.

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Soil microorganisms play key roles in soil phosphorus (P) mobilization in grassland ecosystems. However, little is known about how bacterial communities involved in P transformation respond to soil secondary salinization. In this study, a meadow grassland with a gradient of secondary salinization in the semi-arid Horqin Sandy Land, Northeast China was selected. Soil properties, P fractions, the P transformation potentials, the community structures, and the abundance of a phosphorus (P)-mineralizing gene (phoD) and a P-solubilizing gene (gcd) were determined. NaHCO3-Pi and NaOH-Pi declined with
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21

Bian, Ting, Zhen Wang, Shuang Wang, et al. "Effect of P Reduction on phoD-Harboring Bacteria Community in Solar Greenhouse Soil." Agriculture 14, no. 11 (2024): 1919. http://dx.doi.org/10.3390/agriculture14111919.

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Phosphorus (P) enrichment frequently occurs in the soil used in greenhouse vegetable production (GVP). Minimizing the application of P fertilizer represents a crucial approach to mitigating the accumulation of P in the soil and enhancing its utilization efficiency. However, the changes in bacterial communities and the turnover mechanism of soil P fractions related to soil P cycling after P fertilizer reduction are still unclear. To unravel these complexities, we devised three experimental treatments: conventional nitrogen (N), P, and potassium (K) fertilizer (N1P1K1); conventional N and K fert
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22

Kaneko, Y., Y. Tamai, A. Toh-e, and Y. Oshima. "Transcriptional and post-transcriptional control of PHO8 expression by PHO regulatory genes in Saccharomyces cerevisiae." Molecular and Cellular Biology 5, no. 1 (1985): 248–52. http://dx.doi.org/10.1128/mcb.5.1.248-252.1985.

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A DNA fragment bearing the PHO8 gene, which encodes repressible alkaline phosphatase of Saccharomyces cerevisiae, was cloned. Northern hybridizations with the PHO8 DNA as probe indicated that the PHO8 transcript is 1.8 kilobases in length and is more abundant in cells grown in low-phosphate medium than in high-phosphate medium. The pho9 mutant, whose phenotype is defective in the activity of repressible alkaline phosphatase, produced as much of the PHO8 transcript as did the PHO9+ cells. Hence, the PHO9 product should act at the post-transcriptional level. The pho4 mutant could not derepress t
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23

Kaneko, Y., Y. Tamai, A. Toh-e, and Y. Oshima. "Transcriptional and post-transcriptional control of PHO8 expression by PHO regulatory genes in Saccharomyces cerevisiae." Molecular and Cellular Biology 5, no. 1 (1985): 248–52. http://dx.doi.org/10.1128/mcb.5.1.248.

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A DNA fragment bearing the PHO8 gene, which encodes repressible alkaline phosphatase of Saccharomyces cerevisiae, was cloned. Northern hybridizations with the PHO8 DNA as probe indicated that the PHO8 transcript is 1.8 kilobases in length and is more abundant in cells grown in low-phosphate medium than in high-phosphate medium. The pho9 mutant, whose phenotype is defective in the activity of repressible alkaline phosphatase, produced as much of the PHO8 transcript as did the PHO9+ cells. Hence, the PHO9 product should act at the post-transcriptional level. The pho4 mutant could not derepress t
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24

Masrahi, Abdurrahman, Anil Somenahally, and Terry Gentry. "Interactions of Arbuscular Mycorrhizal Fungi with Hyphosphere Microbial Communities in a Saline Soil: Impacts on Phosphorus Availability and Alkaline Phosphatase Gene Abundance." Soil Systems 4, no. 4 (2020): 63. http://dx.doi.org/10.3390/soilsystems4040063.

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The limited availability of soil phosphorus to plants under salinity stress is a major constraint for crop production in saline soils, which could be alleviated by improving mycorrhizal and soil microbial interactions. This study investigated the effects of Funneliformis mosseae (Fm) inoculation on phosphorus (P) availability to Sorghum bicolor, and alkaline phosphatase (ALP) activity and gene abundance (phoD) in a P-deficient naturally saline soil. A greenhouse study was conducted in order to compare the experimental treatments of Fm inoculated vs. control plants grown in saline soil with and
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25

Barbaric, Slobodan, Martin Münsterkötter, Colin Goding, and Wolfram Hörz. "Cooperative Pho2-Pho4 Interactions at thePHO5 Promoter Are Critical for Binding of Pho4 to UASp1 and for Efficient Transactivation by Pho4 at UASp2." Molecular and Cellular Biology 18, no. 5 (1998): 2629–39. http://dx.doi.org/10.1128/mcb.18.5.2629.

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ABSTRACT The activation of the PHO5 gene in Saccharomyces cerevisiae in response to phosphate starvation critically depends on two transcriptional activators, the basic helix-loop-helix protein Pho4 and the homeodomain protein Pho2. Pho4 acts through two essential binding sites corresponding to the regulatory elements UASp1 and UASp2. Mutation of either of them results in a 10-fold decrease in promoter activity, and mutation of both sites renders the promoter totally uninducible. The role of Pho4 appears relatively straightforward, but the mechanism of action of Pho2 had remained elusive. By i
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26

Ertel, Franziska, A. Barbara Dirac-Svejstrup, Christina Bech Hertel, Dorothea Blaschke, Jesper Q. Svejstrup, and Philipp Korber. "In Vitro Reconstitution of PHO5 Promoter Chromatin Remodeling Points to a Role for Activator-Nucleosome Competition In Vivo." Molecular and Cellular Biology 30, no. 16 (2010): 4060–76. http://dx.doi.org/10.1128/mcb.01399-09.

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ABSTRACT The yeast PHO5 promoter is a classical model for studying the role of chromatin in gene regulation. To enable biochemical dissection of the mechanism leading to PHO5 activation, we reconstituted the process in vitro. Positioned nucleosomes corresponding to the repressed PHO5 promoter state were assembled using a yeast extract-based in vitro system. Addition of the transactivator Pho4 yielded an extensive DNase I-hypersensitive site resembling induced PHO5 promoter chromatin. Importantly, this remodeling was energy dependent. In contrast, little or no chromatin remodeling was detected
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27

Hegyi, Anna, Tran Bao Khuyen Nguyen, and Katalin Posta. "Metagenomic Analysis of Bacterial Communities in Agricultural Soils from Vietnam with Special Attention to Phosphate Solubilizing Bacteria." Microorganisms 9, no. 9 (2021): 1796. http://dx.doi.org/10.3390/microorganisms9091796.

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Bacterial communities can promote increased phosphorus (P) availability for plants and microbes in soil via various mechanisms of phosphate solubilization. The production of extracellular phosphatases releases available P through the hydrolysis of organic P. Examining the abundance and diversity of the bacterial community, including phosphate solubilizing bacteria in soil, may provide valuable information to overcome P scarcity in soil ecosystems. Here, the diversity and relative abundance of bacterial phyla and genera of six agricultural soil samples from Vietnam were analysed by next generat
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28

Wu, Qihua, Wenling Zhou, Diwen Chen, Jiang Tian, and Junhua Ao. "Biochar Mitigates the Negative Effects of Microplastics on Sugarcane Growth by Altering Soil Nutrients and Microbial Community Structure and Function." Plants 13, no. 1 (2023): 83. http://dx.doi.org/10.3390/plants13010083.

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Microplastic pollution in sugarcane areas of China is severe, and reducing the ecological risks is critical. Biochar has been widely used in soil remediation. This study aims to explore the effects and mechanisms of microplastics combined with or without biochar on sugarcane biomass, soil biochemical properties in red soil through a potted experiment. The results show that, compared with control (CK), treatments with microplastics alone reduced the dry biomass of sugarcane, soil pH, and nitrogen (N) and phosphorus (P) contents by an average of 8.8%, 2.1%, 1.1%, and 2.0%, respectively. Interest
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29

Deinert, Lea, and Achim Schmalenberger. "Reuse of Soils Fertilized with Ash as Recycling Derived Fertilizer Revealed Strong Stimulation of Microbial Communities Involved in P Mobilization in Lolium perenne Rhizospheres." Environments 11, no. 3 (2024): 49. http://dx.doi.org/10.3390/environments11030049.

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Circular economy recycling-derived fertilizers (RDF) have the potential to replace linear economy fertilizers such as unsustainable superphosphates. Here, effects of ash RDF treatments in Irish grassland cultivation were investigated in a simulated second growing season. Soil fertilized in a preceding pot trial with superphosphate (SP), poultry-litter ash (PLA) and sewage-sludge ash (SSA) at P concentration of 60 kg P ha−1 and a P-free control (SP0) was reused in a microcosm trial. Lolium perenne was cultivated for 54 days in six replicates with a full complement of micro- and macro-nutrients
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30

Müller, Jörg P., and Manfred Wagner. "Localisation of the cell wall-associated phosphodiesterase PhoD ofBacillus subtilis." FEMS Microbiology Letters 180, no. 2 (1999): 287–96. http://dx.doi.org/10.1111/j.1574-6968.1999.tb08808.x.

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31

Zhang, Kaidian, Jiashun Li, Jie Cheng, and Senjie Lin. "Alkaline Phosphatase PhoD Mutation Induces Fatty Acid and Long-Chain Polyunsaturated Fatty Acid (LC-PUFA)-Bound Phospholipid Production in the Model Diatom Phaeodactylum tricornutum." Marine Drugs 21, no. 11 (2023): 560. http://dx.doi.org/10.3390/md21110560.

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With rapid growth and high lipid contents, microalgae have become promising environmentally friendly candidates for renewable biodiesel and health supplements in our era of global warming and energy depletion. Various pathways have been explored to enhance algal lipid production, especially gene editing. Previously, we found that the functional loss of PhoD-type alkaline phosphatase (AP), a phosphorus-stress indicator in phytoplankton, could lead to increased lipid contents in the model diatom Phaeodactylum tricornutum, but how the AP mutation may change lipid composition remains unexplored. T
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32

Luo, Gongwen, Bo Sun, Ling Li, et al. "Understanding how long-term organic amendments increase soil phosphatase activities: Insight into phoD- and phoC-harboring functional microbial populations." Soil Biology and Biochemistry 139 (December 2019): 107632. http://dx.doi.org/10.1016/j.soilbio.2019.107632.

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33

Li, Jiashun, Kaidian Zhang, Ling Li, Yujie Wang, and Senjie Lin. "Unsuspected functions of alkaline phosphatase PhoD in the diatom Phaeodactylum tricornutum." Algal Research 68 (November 2022): 102873. http://dx.doi.org/10.1016/j.algal.2022.102873.

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34

Xu, Lin, Yongping Kou, Qian Mao, et al. "Climate outweighs fertiliser effects on soil phoD-harbouring communities in agroecosystems." Soil Biology and Biochemistry 202 (March 2025): 109697. https://doi.org/10.1016/j.soilbio.2024.109697.

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35

Liu, Wenbo, Ning Ling, Gongwen Luo, et al. "Active phoD-harboring bacteria are enriched by long-term organic fertilization." Soil Biology and Biochemistry 152 (January 2021): 108071. http://dx.doi.org/10.1016/j.soilbio.2020.108071.

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36

Müller, J. "Localisation of the cell wall-associated phosphodiesterase PhoD of Bacillus subtilis." FEMS Microbiology Letters 180, no. 2 (1999): 287–96. http://dx.doi.org/10.1016/s0378-1097(99)00483-8.

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37

Eder, Steve, Wei Liu, and F. Marion Hulett. "Mutational Analysis of the phoD Promoter in Bacillus subtilis: Implications for PhoP Binding and Promoter Activation of Pho Regulon Promoters." Journal of Bacteriology 181, no. 7 (1999): 2017–25. http://dx.doi.org/10.1128/jb.181.7.2017-2025.1999.

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ABSTRACT The PhoP-PhoR two-component regulatory system controls the phosphate deficiency response in B. subtilis. A number of Pho regulon genes which require PhoP∼P for activation or repression have been identified. The studies reported here were initiated to understand the PhoP-DNA interaction necessary for Pho promoter regulation. The regulatory region of phoD was characterized in detail using oligo-directed mutagenesis, DNase I footprinting, and in vivo transcription assays. These data reveal basic principles of PhoP binding relevant to PhoP’s interaction with other Pho regulon promoters. O
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38

Deinert, Lea, Israel Ikoyi, Bastian Egeter, Patrick Forrestal, and Achim Schmalenberger. "Short-Term Impact of Recycling-Derived Fertilizers on Their P Supply for Perennial Ryegrass (Lolium perenne)." Plants 12, no. 15 (2023): 2762. http://dx.doi.org/10.3390/plants12152762.

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Various nutrient recycling technologies are currently under development in order to alleviate the dependency of non-renewable raw material for the production of mineral phosphorus fertilizers commonly used in agriculture. The resulting products, such as struvites and ashes, need to be assessed for their application as so-called recycling-derived fertilizers (RDFs) in the agricultural sector prior to commercialization. Here, we conducted a short-term (54 days) trial to investigate the impact of different phosphorus fertilizers on plant growth and the soil P cycling microbiota. Lolium perenne wa
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39

Gomez, Peter F., and L. O. Ingram. "Cloning, sequencing and characterization of the alkaline phosphatase gene (phoD) fromZymomonas mobilis." FEMS Microbiology Letters 125, no. 2-3 (1995): 237–45. http://dx.doi.org/10.1111/j.1574-6968.1995.tb07364.x.

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40

Haswell, Elizabeth S., and Erin K. O’Shea. "An In Vitro System Recapitulates Chromatin Remodeling at the PHO5 Promoter." Molecular and Cellular Biology 19, no. 4 (1999): 2817–27. http://dx.doi.org/10.1128/mcb.19.4.2817.

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ABSTRACT The Saccharomyces cerevisiae gene PHO5 is an excellent system with which to study regulated changes in chromatin structure. The PHO5 promoter is packaged into four positioned nucleosomes under repressing conditions; upon induction, the structure of these nucleosomes is altered such that the promoter DNA becomes accessible to nucleases. We report here the development and characterization of an in vitro system in which partially purified PHO5 minichromosomes undergo promoter chromatin remodeling. Several hallmarks of thePHO5 chromatin transition in vivo were reproduced in this system. C
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41

Noskova, Yulia, Galina Likhatskaya, Natalia Terentieva, Oksana Son, Liudmila Tekutyeva, and Larissa Balabanova. "A Novel Alkaline Phosphatase/Phosphodiesterase, CamPhoD, from Marine Bacterium Cobetia amphilecti KMM 296." Marine Drugs 17, no. 12 (2019): 657. http://dx.doi.org/10.3390/md17120657.

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A novel extracellular alkaline phosphatase/phosphodiesterase from the structural protein family PhoD that encoded by the genome sequence of the marine bacterium Cobetia amphilecti KMM 296 (CamPhoD) has been expressed in Escherichia coli cells. The calculated molecular weight, the number of amino acids, and the isoelectric point (pI) of the mature protein’s subunit are equal to 54832.98 Da, 492, and 5.08, respectively. The salt-tolerant, bimetal-dependent enzyme CamPhoD has a molecular weight of approximately 110 kDa in its native state. CamPhoD is activated by Co2+, Mg2+, Ca2+, or Fe3+ at a co
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42

Bai, Shanshan, Yifei Ge, Dongtan Yao, et al. "Effect of straw retention and mineral fertilization on P speciation and P-transformation microorganisms in water- extractable colloids of a Vertisol." Biogeosciences 22, no. 1 (2025): 135–51. https://doi.org/10.5194/bg-22-135-2025.

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Abstract. Water-extractable colloids (WECs) serve as crucial micro-particulate components in soils, playing a vital role in the cycling and potential bioavailability of soil phosphorus (P). Yet, the underlying information regarding soil P species and P-transformation microorganisms at the microparticle scale under long-term straw retention and mineral fertilization is barely known. Here, a fixed field experiment (∼ 13 years) in a Vertisol was performed to explore the impacts of straw retention and mineral fertilization on inorganic P, organic P, and P-transformation microorganisms in bulk soil
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43

Wang, Chaoqun, Lin Xue, and Ruzhen Jiao. "Soil phosphorus fractions, phosphatase activity, and the abundance of phoC and phoD genes vary with planting density in subtropical Chinese fir plantations." Soil and Tillage Research 209 (May 2021): 104946. http://dx.doi.org/10.1016/j.still.2021.104946.

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Fraser, Tandra D., Derek H. Lynch, Jonathan Gaiero, Kamini Khosla, and Kari E. Dunfield. "Quantification of bacterial non-specific acid ( phoC) and alkaline ( phoD ) phosphatase genes in bulk and rhizosphere soil from organically managed soybean fields." Applied Soil Ecology 111 (March 2017): 48–56. http://dx.doi.org/10.1016/j.apsoil.2016.11.013.

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45

Wei, Xiaomeng, Yajun Hu, Guan Cai, et al. "Organic phosphorus availability shapes the diversity of phoD-harboring bacteria in agricultural soil." Soil Biology and Biochemistry 161 (October 2021): 108364. http://dx.doi.org/10.1016/j.soilbio.2021.108364.

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Hu, Yajun, Yinhang Xia, Qi Sun, et al. "Effects of long-term fertilization on phoD-harboring bacterial community in Karst soils." Science of The Total Environment 628-629 (July 2018): 53–63. http://dx.doi.org/10.1016/j.scitotenv.2018.01.314.

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Zhang, Tingxi, Mengyao Qin, Chao Wei, Defang Li, Xiaoran Lu, and Limin Zhang. "Suspended particles phoD alkaline phosphatase gene diversity in large shallow eutrophic Lake Taihu." Science of The Total Environment 728 (August 2020): 138615. http://dx.doi.org/10.1016/j.scitotenv.2020.138615.

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48

Tait-Kamradt, A. G., K. J. Turner, R. A. Kramer, et al. "Reciprocal regulation of the tandemly duplicated PHO5/PHO3 gene cluster within the acid phosphatase multigene family of Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 6 (1986): 1855–65. http://dx.doi.org/10.1128/mcb.6.6.1855-1865.1986.

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We characterized the organization and expression of PHO5 and PHO3, the tightly linked repressible and constitutive acid phosphatase genes of Saccharomyces cerevisiae. The "constitutive" gene, PHO3, is expressed only when PHO5 is not. Altering PHO5 expression, either through promoter deletions or through mutations in trans-acting regulatory genes, showed that PHO5 expression is sufficient to block transcription of PHO3. An active genomic copy of PHO5 was able to block expression of PHO3 from a high-copy-number plasmid, showing that some trans-acting product of PHO5 is involved. This is probably
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49

Tait-Kamradt, A. G., K. J. Turner, R. A. Kramer, et al. "Reciprocal regulation of the tandemly duplicated PHO5/PHO3 gene cluster within the acid phosphatase multigene family of Saccharomyces cerevisiae." Molecular and Cellular Biology 6, no. 6 (1986): 1855–65. http://dx.doi.org/10.1128/mcb.6.6.1855.

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We characterized the organization and expression of PHO5 and PHO3, the tightly linked repressible and constitutive acid phosphatase genes of Saccharomyces cerevisiae. The "constitutive" gene, PHO3, is expressed only when PHO5 is not. Altering PHO5 expression, either through promoter deletions or through mutations in trans-acting regulatory genes, showed that PHO5 expression is sufficient to block transcription of PHO3. An active genomic copy of PHO5 was able to block expression of PHO3 from a high-copy-number plasmid, showing that some trans-acting product of PHO5 is involved. This is probably
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50

Neef, Daniel W., and Michael P. Kladde. "Polyphosphate Loss Promotes SNF/SWI- and Gcn5-Dependent Mitotic Induction of PHO5." Molecular and Cellular Biology 23, no. 11 (2003): 3788–97. http://dx.doi.org/10.1128/mcb.23.11.3788-3797.2003.

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ABSTRACT Approximately 800 transcripts in Saccharomyces cerevisiae are cell cycle regulated. The oscillation of ∼40% of these genes, including a prominent subclass involved in nutrient acquisition, is not understood. To address this problem, we focus on the mitosis-specific activation of the phosphate-responsive promoter, PHO5. We show that the unexpected mitotic induction of the PHO5 acid phosphatase in rich medium requires the transcriptional activators Pho4 and Pho2, the cyclin-dependent kinase inhibitor Pho81, and the chromatin-associated enzymes Gcn5 and Snf2/Swi2. PHO5 mitotic activation
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