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Journal articles on the topic 'Phosphate buffered saline (PBS)'

Author: Grafiati

Published: 5 June 2025

Last updated: 16 July 2025

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1

Wieczorowska–Tobis, Katarzyna, Arkadiusz Styszynski, Andrzej Breborowicz, and Dimitrios G. Oreopoulos. "Comparison of the Biocompatibility of Phosphate-Buffered Saline Alone, Phosphate-Buffered Saline Supplemented with Glucose, and Dianeal 3.86%." Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis 21, no. 3_suppl (2001): 362–64. http://dx.doi.org/10.1177/089686080102103s68.

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Objective We compared the effects of intraperitoneal infusion of phosphate-buffered saline (PBS, pH 7.4), of PBS supplemented with 3.86% glucose (G), and of standard dialysis solution [Dianeal 3.86%: Baxter Healthcare Corporation, Deerfield, IL, U.S.A. (D)] on intraperitoneal inflammation in dialyzed rats. Methods After catheter implantation, rats were infused on day 1 with PBS, on day 3 with PBS+G, on day 5 with D, and on day 7 again with PBS (PBS-2). After a 4-hour dwell, dialysate samples were collected and analyzed. Results All dialysate parameters studied [dialysate cell count, neutrophil

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Ye, Qianyu, Matthew Biviano, Srinivas Mettu, Meifang Zhou, Raymond Dagastine, and Muthupandian Ashokkumar. "Modification of pea protein isolate for ultrasonic encapsulation of functional liquids." RSC Advances 6, no. 108 (2016): 106130–40. http://dx.doi.org/10.1039/c6ra17585f.

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This study reports on the ultrasonic processing of pea protein isolate (PPI) in phosphate-buffered saline (PBS, pH 7.4) and Tris/HCl (pH 8) buffer systems in order to modify its properties for use in the encapsulation of functional liquids.

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3

Jumper, T. M., M. Thoresen, E. H. King, D. R. Smith, and D. Loy. "Comparing 0.9% sterile saline to phosphate buffered saline as a transport media for Tritrichomonas foetus RT-qPCR testing." American Association of Bovine Practitioners Conference Proceedings 58, no. 58 (2025): 201. https://doi.org/10.21423/aabppro20249150.

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The limit of detection for T. foetus RT-qPCR is 1 organism/extraction in phosphate buffered saline (PBS) without prior culture. The objective of this study was to determine if 0.9% sterile saline (SAL), a readily available media, was not inferior to PBS as a transport media for T. foetus RT-qPCR testing at that concentration.

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McDaniel, Jennifer S., Barbara Wirostko, Cassie Sprague, Anthony J. Johnson, and Gina L. Griffith. "The Effects of Phosphate Buffered Saline on Corneal Chemical Alkali Burns." Journal of Ocular Diseases and Therapeutics 5 (February 27, 2017): 28–34. http://dx.doi.org/10.12974/2309-6136.2017.05.05.

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Background: Treatments administered after a corneal burn injury can have long term implications on wound healing. Initial treatments can include the administration of lubricants, anti inflammatories, and antibiotic eye drops, many of which contain phosphate buffered saline (PBS) as a vehicle. As a result, the purpose of this study was to observe the effects of the commonly used PBS ophthalmic drop vehicle on corneal alkali burns over 30 days when treated 4X daily for the first 14 days. Materials and Methods: Corneal alkali burns 5.5 mm in diameter were created on 10 anesthetized, male New Zeal

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Wang, Quan Xiang, Xie Ping Dong, Xiao Feng Tang, et al. "Magnesium Phosphate/Polybutylene Succinate Composite for Bone Repair." Advanced Materials Research 1102 (May 2015): 95–98. http://dx.doi.org/10.4028/www.scientific.net/amr.1102.95.

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MP/PBS composite was fabricated by melt blending method. The weight loss ratio of MP/PBS composite reached 35.43 wt.% after immersion into phosphate buffered saline solution for 12 weeks, and the pH change from 7.40 to 7.72. The relative proliferation rate of cells on the materials increased with time and the proliferation of the cells on the MP/PBS composite was significantly higher than PBS. The results reveal MP/PBS composite has good biocompatibility, which satisfied the relevant criterion on biomaterials.

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Aavik, A., R. T. Kibur, J. Lieberg, U. Lepner, M. Aunapuu, and A. Arend. "Cold-Stored Venous Allografts In Different Preserving Solutions: A Study On Changes In Vein Wall Morphology." Scandinavian Journal of Surgery 108, no. 1 (2018): 67–75. http://dx.doi.org/10.1177/1457496918783728.

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Background: The saphenous vein is the most frequently used bypass conduit for vascular reconstructions, which may need to be stored for a prolonged time. The aim of this study was to compare the effect of different preservation solutions on the morphology of saphenous veins during the long-term cold storage. Design: An in vitro study Material and Methods: Saphenous vein samples, collected from 29 patients undergoing varicose vein surgery, were stored at +4°C in (1) 10% formalin, (2) isotonic saline with heparin and antibiotic, (3) phosphate-buffered saline, (4) 2.5% glutaraldehyde + phosphate-

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Lin, Lin, Ashraf S. Ibrahim, Valentina Avanesian, et al. "Considerable Differences in Vaccine Immunogenicities and Efficacies Related to the Diluent Used for Aluminum Hydroxide Adjuvant." Clinical and Vaccine Immunology 15, no. 3 (2008): 582–84. http://dx.doi.org/10.1128/cvi.00427-07.

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ABSTRACT We are developing an anticandidal vaccine using the recombinant N terminus of Als3p (rAls3p-N). We report that although more rAls3p-N was bound by aluminum hydroxide diluted in saline than by aluminum hydroxide diluted in phosphate-buffered saline (PBS), its immunogenicity and efficacy were superior in PBS. Thus, protein binding, by itself, may not predict the efficacy of some vaccines with aluminum adjuvants.

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Nugraha, Gilang, Diyan Wahyu Kurniasari, Mita Rahayu, Aulia Sabrina, Alya Salsabila, and Muhammad Rizqi Cahyadi. "UJI LARUTAN SAFRANIN – PHOSPHATE BUFFERED SALINE SEBAGAI REAGEN ALTERNATIF HITUNG ERITROSIT DAN TROMBOIT." Jurnal Medika 9, no. 2 (2024): 51–55. https://doi.org/10.53861/jmed.v9i2.464.

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Manual examination of RBC and PLT was carried out using a microscope using different reagents, namely Hayem and Rees Ecker. We formulated a diluent solution consisting of PBS and Safranin for RBC and PLT examination at the same time. Therefore, we tried to test our Safranin – PBS solution formula so that it could be used to determine RBC and PLT simultaneously. The research involved 10 respondents with blood testing units. RBC and PLT were measured using a hematology analyzer (control) and Safranin – PBS pH 6.8 and 7.0 (treatment). The results of microscopic observations of PLT appear to be mo

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Ondaral, Sedat, Elif Çelik, and Orçun Çağlar Kurtuluş. "The adsorption of phosphate-buffered saline to model films composed of nanofibrillated cellulose and gelatin." Journal of Applied Biomaterials & Functional Materials 17, no. 1 (2019): 228080001982651. http://dx.doi.org/10.1177/2280800019826513.

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Phosphate-buffered saline (PBS) adsorption on model films composed of gelatin and nanofibrillated celluloses (NFCs) with different aldehyde and carboxyl contents was studied by means of the quartz crystal microbalance with dissipation (QCM-D) technique in this study. The results showed that frequency shift (Δ f) due to PBS adsorption increased with increasing gelatin content to 50% in NFC-containing films. The dissipation shift and adsorption rate of PBS followed the same trend. Model films with NFC-1 having 1.22 mmol/g aldehyde and 0.6 mmol/g carboxyl groups adsorbed more PBS than those consi

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Sultan, S. M., M. R. R. de Planque, P. Ashburn, and H. M. H. Chong. "Effect of Phosphate Buffered Saline Solutions on Top-Down Fabricated ZnO Nanowire Field Effect Transistor." Journal of Nanomaterials 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/5413705.

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ZnO based nanowire FETs have been fabricated by implementing a top-down approach, which uses optical photolithography, atomic layer deposition (ALD) of ZnO thin film, and anisotropic plasma etching. The effects of Phosphate Buffered Saline (PBS) solution on the surface of ZnO nanowire were investigated by measuring the FET characteristics at different PBS dilutions. The drain current, ION, exhibited an increase of 39 times in the highest PBS solution concentration compared to measurement in air. From the measured transfer characteristics and output characteristics in various PBS dilutions, the

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Cabeza, Sandra, Pablo Pérez Zubiaur, Gerardo Garcés, Carmen Andrade, and Paloma Adeva. "Corrosion Behaviour of Mg98.5Nd1Zn0.5 (at. %) Alloy in Phosphate Buffered Saline Solution." Metals 10, no. 1 (2020): 148. http://dx.doi.org/10.3390/met10010148.

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The corrosion behaviour of Mg98.5-Nd1-Zn0.5 (at. %) alloy was studied in phosphate buffered saline (PBS) solution to evaluate its degradation performance as a potential candidate for biomedical applications. The alloy, produced by casting and hot extrusion, consists of a fine-grained magnesium matrix with an average grain size of 3.8 μm embedding a high volume fraction of (Mg, Zn)12Nd precipitates. Hydrogen release tests revealed a stable low corrosion rate of 0.6 mm/year after 24 h of immersion. Electrochemical testing data proved good correlation with the data from hydrogen evolution, with t

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Yilmazer, Hakan, Ihsan Caha, Burak Dikici, et al. "Investigation of the Influence of High-Pressure Torsion and Solution Treatment on Corrosion and Tribocorrosion Behavior of CoCrMo Alloys for Biomedical Applications." Crystals 13, no. 4 (2023): 590. http://dx.doi.org/10.3390/cryst13040590.

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In this study, the influence of the high-pressure torsion (HPT) processing parameters and solution treatment (ST) on the corrosion and tribocorrosion behavior of CoCrMo (CCM) alloys was investigated for possible usage in biomedical applications. The corrosion behavior of the CCM alloys was investigated by using potentiodynamic scanning (PDS) and electrochemical impedance spectroscopy (EIS) tests. Tribocorrosion tests were carried out in a reciprocating ball-on-plate tribometer at 1 Hz, 1 N load, and 3 mm stroke length for 2 h. All electrochemical measurements were performed using a potentiosta

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Botelho, Paula Mauro, Marina Carvalho Prado, Nancy Kudsi Carvalho, Edson Jorge Lima Moreira, and Emmanuel João Nogueira Leal da Silva. "Influence of MTA powder handled with distilled water or phosphate buffered saline in push-out performance." Revista Brasileira de Odontologia 75 (August 6, 2018): 1. http://dx.doi.org/10.18363/rbo.v75.2018.e1104.

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Objetivo: investigar a resistência de união do pó de MTA manipulado com água destilada (DW) ou solução salina tamponada com fosfato (PBS). Material e Métodos: dois cortes transversais horizontais foram preparados na raiz de quinze incisivos, seguido de perfuração de dois orifícios em forma de canal na sua superfície axial. Os buracos foram preenchidos com MTA com DW ou PBS. A carga foi aplicada até o deslocamento do MTA. Testes de Mann-Whitney e Friedman foram selecionados para estabelecer a influência de variáveis independentes na força de push-out. Resultados: o MTA manipulado com DW e PBS a

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Silva-Neto, A. V., W. G. Santos, A. F. M. Botelho, G. M. L. Diamantino, B. Soto-Blanco, and M. M. Melo. "Use of EDTA in the treatment of local tissue damage caused by the Bothrops alternatus venom." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 70, no. 5 (2018): 1529–38. http://dx.doi.org/10.1590/1678-4162-10158.

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ABSTRACT Twelve adult rabbits were distributed in three groups and received on the femoral biceps region, via intradermal injection (ID), 25µg of Bothrops alternatus venom dissolved in NaCl 0.9% and diluted in 0.25mL of phosphate buffered saline (PBS). Thirty minutes later, the group G1 received 0.25mL of phosphate buffered saline (PBS) ID while to G2 and G3 25mg of ethylenediamine tetraacetic acid (EDTA) dissolved in 0.25mL of PBS were administered via intramuscular (IM) and intravenous (IV) injection, respectively. Evaluations included local lesion and blood profile of all animals, before (t

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15

Gudić, Senka, Ladislav Vrsalović, Dario Kvrgić, and Aleš Nagode. "Electrochemical Behaviour of Ti and Ti-6Al-4V Alloy in Phosphate Buffered Saline Solution." Materials 14, no. 24 (2021): 7495. http://dx.doi.org/10.3390/ma14247495.

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The electrochemical behavior of commercially pure titanium (CP Ti) and Ti-6Al-4V (Grade 5) alloy in phosphate buffered saline solution (PBS, pH = 7.4) at 37 °C (i.e., in simulated physiological solution in the human body) was examined using open circuit potential measurements, linear and potentiodynamic polarization and electrochemical impedance spectroscopy methods. After the impedance measurements and after potentiodynamic polarization measurements, the surface of the samples was investigated by scanning electron microscopy, while the elemental composition of oxide film on the surface of eac

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Asadi, Ali. "Design and fabrication of passive micromixer for synthesis of Phosphate-Buffered Saline (PBS)." JOURNAL OF CHARACTERIZATION 1, no. 1 (2025): 27–37. https://doi.org/10.29228/jchar.80393.

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Griseti, Elena, Nofel Merbahi, and Muriel Golzio. "Anti-Cancer Potential of Two Plasma-Activated Liquids: Implication of Long-Lived Reactive Oxygen and Nitrogen Species." Cancers 12, no. 3 (2020): 721. http://dx.doi.org/10.3390/cancers12030721.

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Cold atmospheric plasma-exposed culture medium may efficiently kill cancer cells in vitro. Due to the complexity of the medium obtained after plasma exposure, less complex physiological liquids, such as saline solutions and saline buffers, are gathering momentum. Among the plethora of reactive oxygen and nitrogen species (RONS) that are produced in these plasma-activated liquids, hydrogen peroxide, nitrite and nitrate appear to be mainly responsible for cytotoxic and genotoxic effects. Here, we evaluated the anti-cancer potential of plasma-activated phosphate-buffered saline (P-A PBS) and sodi

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Teng, Juan, Da Yong Gu, Yun Qing Xu, et al. "Screening Method for Real-Time Detection of Influenza-A Virus in Human Throat Swabs by Surface Plasmon Resonance Biosensor." Applied Mechanics and Materials 140 (November 2011): 210–19. http://dx.doi.org/10.4028/www.scientific.net/amm.140.210.

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A surface plasmon resonance (SPR) biosensor was applied to detect influenza-A virus in human. The detection scheme was based on the measurement of SPR response unit resulting from the hybridization of biotinylated DNA probe immobilized on the SPR chip modified with streptavidin and the product of polymerase chain reaction reversed from the influenza-A virus RNA segment (AB514942). The prepared biosensor demonstrated optimum performance in 200 mM phosphate buffered saline (PBS) with a pH value of 7.5 and exhibited good sensitivity with a detection limit of 0.5 pM for perfect complementary hybri

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Yu, Zai, Shi Chuan Tang, Peng Zhao, Ru Gang Zhong, and Bing Chang. "Dispersion and Stability of Nanoalumina in Solution Prior to Exposure." Advanced Materials Research 850-851 (December 2013): 16–19. http://dx.doi.org/10.4028/www.scientific.net/amr.850-851.16.

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Dispersion of nanoalumina in normal saline (NS) and phosphate buffered saline (PBS) was studied. The effects of different dispersing methods were investigated. The results showed nanoalumina was better dispersed in NS than PBS. And sonication was a more effective method that helped deaggregation compared with vortex oscillation. Finally, the influence of sonication time on the particle size and the stability of the sonicated NS suspension were studied using dynamic light scattering. The aggregation size was decreased to about 130 nm after ten-minute sonication and maintained when increasing so

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Mohammad, Nur Farahiyah, Koo Li Mei, Mohd Riza Mohd Roslan, Siti Shuhadah Md Saleh, and Farah Diana Mohd Daud. "Synthesis and Characterization of Nanoporous Biphasic Calcium Phosphate." Journal of Physics: Conference Series 2071, no. 1 (2021): 012009. http://dx.doi.org/10.1088/1742-6596/2071/1/012009.

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Abstract For the past few years, many researchers are focusing on biomaterials fabrication in porous form. The research on porous calcium phosphate has been investigated due to its excellent biocompatibility and better osseointegration. This research paper presented nanoporous biphasic calcium phosphate (BCP) synthesised using chemical precipitation method. Triblock co-polymer F127 was used as pore directing agent. The chemical compositions of pure BCP samples were examined using X-ray diffraction (XRD) analysis which shows common peak of BCP. The pore size distribution (PSD) on the other hand

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Wang, Mei Ping, Jin Hui Lin, and Xiao Li Hao. "Preparation and Characterization of CdTe/SiO₂ Composite." Advanced Materials Research 233-235 (May 2011): 1946–49. http://dx.doi.org/10.4028/www.scientific.net/amr.233-235.1946.

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TGA- stabilized CdTe nanocrystals (NCs) were prepared using sodium tellurite as tellurium source, which avoids the cumbersome processes associated with H2Te or NaHTe sources. Fluorescent CdTe/SiO2composites were prepared by a sol–gel method without the exchange of surface ligands. The composites were characterized with TEM and fluorescence spectrophotometer and investigated their stability in phosphate-buffered saline (PBS) buffer. CdTe NCs were loaded into the core of silica spheres during the formation of composite spheres. In contrast to CdTe NCs in aqueous solutions, CdTe NCs in the compos

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Wang, D., P. Clement, J. Smitz, and M. P. Derde. "Relevance of histamine and tryptase concentrations in nasal secretions after nasal challenges with phosphate buffered saline and allergen." Mediators of Inflammation 4, no. 2 (1995): 98–102. http://dx.doi.org/10.1155/s0962935195000160.

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In this prospective study, a quantitative determination of histamine and tryptase in nasal secretions after nasal phosphate buffered saline (PBS) and allergen challenge was performed in 18 atopic patients who were compared with ten non-allergic healthy volunteers. The aim of the study was to determine the normal and pathological concentrations of these important mediators in nasal secretions. The second objective was to test the relevance of these two mast cell secreted mediators after nasal challenge. Results showed that the concentrations of tryptase in almost all samples were under the mini

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Huang, Weidong, Xiufeng Xie, Xinyue Liang, Xingmin Wang, and Xiaosheng Chen. "Effects of Different Pretreatments of DNA Extraction from Dried Specimens of Ladybird Beetles (Coleoptera: Coccinellidae)." Insects 10, no. 4 (2019): 91. http://dx.doi.org/10.3390/insects10040091.

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Obtaining genetic information from museum specimens is a fundamental component of many fields of research, including DNA barcoding, population genetics, conservation genetics, and phylogenetic analysis. However, acquiring genetic information from museum specimens is challenging because of the difficulty in amplifying the target sequences due to DNA damage and degradation. Different pretreatments can significantly impact the purity and concentration of genomic DNA from museum specimens. Here, we assessed four pretreatment methods—use of 0.9% NaCl buffer, phosphate-buffered saline (PBS), Saline

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Ross, Kristen R., and Michael E. Berens. "Hold-'Em In Place." Microscopy Today 9, no. 2 (2001): 18–21. http://dx.doi.org/10.1017/s1551929500056418.

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We've used gelled agarose to cover & hold monolayer cells in place when treatment leads to cell lifting. Low-gelling temperature agarose (Type VII) (Sigma A 4018 or A 0701) can be made as a 2% stock solution in “phosphate-buffered saline” (PBS) (boiled then kept at 42° C to remain liquid). The agarose can be stored at room temperature at this time, but must be boiled and melted again before use.

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Ueda, Kyosuke, Takayuki Narushima, Tomoyuki Katsube, Hiroshi Kawamura, and Takashi Goto. "In Vitro Evaluation of RF Magnetron-Sputtered Calcium Phosphate Films on Titanium." Key Engineering Materials 352 (August 2007): 305–9. http://dx.doi.org/10.4028/www.scientific.net/kem.352.305.

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Calcium phosphate coating films were fabricated on mirror-polished or blast-treated titanium substrates using radio-frequency (RF) magnetron sputtering and they were evaluated in vitro. Immersion tests for the films were conducted using phosphate-buffered saline (PBS(-)), and apatite formation and the elution of calcium ions from the films were investigated. The bonding strengths between the calcium phosphate films and titanium substrates before and after the immersion tests were evaluated. After the immersion tests, a decrease in the bonding strength was observed for the coating films on the

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Banu, Melania, Monica Simion, Mihaela Kusko, and Ileana C. Farcasanu. "Enhancing the Microarray Signal Detection of Single Nucleotide Polymorphisms (SNPs) by Using Homemade Immobilisation Buffers." Revista de Chimie 70, no. 2 (2019): 730–35. http://dx.doi.org/10.37358/rc.19.2.6995.

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Our research is focused on improving the detection capabilities of single nucleotide polymorphisms (SNPs) with pathological significance within breast cancer 1, early onset gene (BRCA1). We employed the BRCA1 canonical probe sequence containing the common C allele and probes including the mismatches caused by C replacement with any of the other nucleotides (A, G or T). Two immobilization buffers with distinct pH and salt concentration were involved in the experimental and statistical analyses in order to assess the detection specificity and stability over time of the tethered probes in oligonu

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Kubiak, Robert J., Nancy Lee, Yuan Zhu, et al. "Storage Conditions of Conjugated Reagents Can Impact Results of Immunogenicity Assays." Journal of Immunology Research 2016 (2016): 1–10. http://dx.doi.org/10.1155/2016/1485615.

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Consistent performance of anti-drug antibody (ADA) assays through all stages of clinical development is critical for the assessment of immunogenicity and interpretation of PK, PD, safety, and efficacy. The electrochemiluminescent assays commonly employed for ADA measurement use drug conjugated with ruthenium and biotin to bind ADA in samples. Here we report an association between high nonspecific ADA responses in certain drug-naïve individuals and the storage buffer of the conjugated reagents used in a monoclonal antibody ADA assay. Ruthenylated reagents stored in phosphate-buffered saline (PB

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Filipič, Bratko, Klemen Rihar, Dunja Exel Gregorič та ін. "Enhancing Effect of 100.414-kHz Electromagnetic Field Produced by Defender’s Pulse Generator on the ChIFN γ-Like Molecule Inducing Capacity of Lens culinaris Agglutinin and 10% PBS Washouts of Different Holocene Minerals". Technology in Cancer Research & Treatment 18 (1 січня 2019): 153303381882109. http://dx.doi.org/10.1177/1533033818821093.

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Macrophages play key role in host defense and tissue repair, and thus understanding regulation of their function is important. For instance, our previous results have shown that in chicken macrophage system (CoMA cell line), application of a pulse of electromagnetic fields of frequencies 0.618, 1.054, 5.229, and 100.414 kHz induces production of interferon γ-like molecules. In this study, we have shown that the electromagnetic field of 100.414 kHz is the most effective in inducing synthesis of chicken interferon γ and chicken interferon γ-like molecules in CoMA cells, especially when combined

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Ross, Christopher, Basir Syed, Joanna Pak, Vishal Jhanji, Jason Yamaki, and Ajay Sharma. "Stability Evaluation of Extemporaneously Compounded Vancomycin Ophthalmic Drops: Effect of Solvents and Storage Conditions." Pharmaceutics 13, no. 2 (2021): 289. http://dx.doi.org/10.3390/pharmaceutics13020289.

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Vancomycin is the drug of choice for methicillin-resistant Staphylococcus aureus keratitis and other ocular infections. Vancomycin ophthalmic drops are not commercially available and require compounding. The present study was designed to investigate the stability of vancomycin ophthalmic drops in normal saline, phosphate-buffered saline (PBS), and balanced salt solution (BSS) while stored at room temperature or under refrigeration. Vancomycin ophthalmic drops (50 mg/mL) were aseptically prepared from commercially available intravenous powder using PBS, BSS, and saline. Solutions were stored at

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Bekeschus, Sander, André Käding, Tim Schröder, et al. "Cold Physical Plasma-Treated Buffered Saline Solution as Effective Agent Against Pancreatic Cancer Cells." Anti-Cancer Agents in Medicinal Chemistry 18, no. 6 (2018): 824–31. http://dx.doi.org/10.2174/1871520618666180507130243.

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Background: Cold physical plasma has been suggested as a new anticancer tool recently. However, direct use of plasma is limited to visible tumors and in some clinical situations, is not feasible. This includes repetitive treatment of peritoneal metastases, which commonly occur in advanced gastrointestinal cancer and in pancreatic cancer in particular. In case of diffuse intraperitoneal metastatic spread, Hyperthermic Intraperitoneal Intraoperative Chemotherapy (HIPEC) is used as a therapeutic approach. Plasma-treated solutions may combine non-toxic characteristics with the anticancer effects o

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BOGUŃ, MACIEJ, KATARZYNA ŚLEDZIŃSKA, MAŁGORZATA GIEŁDOWSKA, PAULINA KRÓL, IRENA KAMIŃSKA, and SYLWIA MAGDZIARZ. "POLYACRYLONITRILE NONWOVENS FOR THE PRODUCTION OF CARBON MATERIALS SUPPORTING THE REGENERATION OF BONE AND CARTILAGE TISSUES." Fibres and Textiles 30, no. 1 (2023): 131–33. http://dx.doi.org/10.15240/tul/008/2023-1-023.

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The influence of the change in surface weight on the physical properties of oxidized polyacrylonitrile precursor nonwovens intended for the production of carbon materials used in tissue engineering was studied. Thermal insulation properties of the nonwovens and their behavior during incubation in phosphate buffered saline (PBS) were investigated. Initial carbonization tests showed that from the point of view of carbonization and further application of carbon materials, the most effective was the use of a surface weight of about 120 g/m2. At the same time, for the research conducted on the incu

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Ditshego, Nonofo M. J. "Highly Sensitive ZnO NWFET Biosensor Fabricated Using Top-Down Processes." Journal of Nano Research 55 (November 2018): 66–74. http://dx.doi.org/10.4028/www.scientific.net/jnanor.55.66.

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A highly sensitive low-doped ZnO nanowire field effect transistor (NWFET) biosensor has been fabricated and measured. The low doped biosensor with NWFET transducer was used to sense charge of the following substances: lysozyme (LYSO), phosphate buffered saline (PBS), bovine serum albumin (BSA). It achieved maximum sensitivity of -543.2 % for the PBS-LYSO protein and 13,069 % for the PBS-BSA protein. These results were achieved because the electrical measurement and characterisation was focused on the charge effect of the LYSO and BSA acting on the ZnO nanowire subthreshold region. The nano-fab

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Gong, Yan, Wentai Liu, Runyu Wang, Matthew Harris Brauer, Kristine Zheng, and Wen Li. "Stability Performance Analysis of Various Packaging Materials and Coating Strategies for Chronic Neural Implants under Accelerated, Reactive Aging Tests." Micromachines 11, no. 9 (2020): 810. http://dx.doi.org/10.3390/mi11090810.

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Reliable packaging for implantable neural prosthetic devices in body fluids is a long-standing challenge for devices’ chronic applications. This work studied the stability of Parylene C (PA), SiO2, and Si3N4 packages and coating strategies on tungsten wires using accelerated, reactive aging tests in three solutions: pH 7.4 phosphate-buffered saline (PBS), PBS + 30 mM H2O2, and PBS + 150 mM H2O2. Different combinations of coating thicknesses and deposition methods were studied at various testing temperatures. Analysis of the preliminary data shows that the pinholes/defects, cracks, and interfac

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Diederichs, Stella, Dayan Nugmanov, Yulia Ivanisenko, and Eberhard Kerscher. "Corrosion Activity of Ultrafine-Grained Pure Magnesium and ZK60 Magnesium Alloy in Phosphate Buffered Saline Solution." Materials 17, no. 11 (2024): 2726. http://dx.doi.org/10.3390/ma17112726.

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The magnesium alloy ZK60 is a promising candidate as a material for biodegradable implants. One of the most important factors for biodegradable implants is the modification of their corrosion behavior to match the requirements for the healing bone or tissue. The corrosion behavior can be influenced by different factors, among them the grain size, which can be changed by severe plastic deformation processes such as High Pressure Torsion Extrusion (HPTE). This study focuses on the corrosion behavior of samples of pure magnesium and ZK60 before and after HPTE, and the influence of the microstruct

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Ferwana, O. S., S. C. Pirie, and D. J. Potts. "Effect of Phosphate-Buffered Sucrose Flush Solution upon the Initial Phase of Ischaemic Acute Renal Failure in the Rat." Clinical Science 77, no. 1 (1989): 77–84. http://dx.doi.org/10.1042/cs0770077.

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1. Experiments were performed upon anaesthetized rats to investigate the effect upon the initial 4 h of ischaemic acute renal failure of intrarenal flush with phosphate buffer (PB), sucrose alone and phosphate-buffered sucrose (PBS) solutions of differing compositions. 2. Kidneys flushed with PB immediately before 45 min pedicle clamp ischaemia exhibited a post-ischaemic function similar to that of control, non-flushed kidneys: they were isosthenuric and non-oliguric with a clearance of inulin (Cin) depressed to 10% of the pre-ischaemic value. 3. Kidneys flushed with 50 mmol/l sucrose in salin

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Gu, W. Y., and M. A. Justiz. "Apparatus for Measuring the Swelling Dependent Electrical Conductivity of Charged Hydrated Soft Tissues." Journal of Biomechanical Engineering 124, no. 6 (2002): 790–93. http://dx.doi.org/10.1115/1.1516571.

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This paper describes a new apparatus and method for measuring swelling dependent electrical conductivity of charged hydrated soft tissues. The apparatus was calibrated using a conductivity standard. Swelling dependent specific conductivity of porcine annulus fibrosis (AF) samples was determined. The conductivity values for porcine AF were similar to those for human and bovine articular cartilage found in the literature. Results revealed a significant linear correlation between specific conductivity and water content for porcine AF tissues tested in phosphate buffered saline (PBS).

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KOO, JAHEON, MICHAEL L. JAHNCKE, PAUL W. RENO, XIAOPEI HU, and PARAMESWARAKUMAR MALLIKARJUNAN. "Inactivation of Vibrio parahaemolyticus and Vibrio vulnificus in Phosphate-Buffered Saline and in Inoculated Whole Oysters by High-Pressure Processing." Journal of Food Protection 69, no. 3 (2006): 596–601. http://dx.doi.org/10.4315/0362-028x-69.3.596.

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Inactivation studies for Vibrio parahaemolyticus TX-2103 (serotype O3:K6) and Vibrio vulnificus MO-624 (clinical isolate) were conducted in phosphate-buffered saline (PBS) and in inoculated oysters under high-pressure processing conditions. V. parahaemolyticus was more resistant than V. vulnificus in PBS at all pressures and times. A 6-log reduction of V. parahaemolyticus and V. vulnificus in PBS at 241 MPa required 11 and 5 min, respectively, which included a 3-min pressure come-up time. A 4.5-log reduction of V. parahaemolyticus in oysters at 345 MPa required 7.7 min, which included a 6.7-mi

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Jacob, J., and M. F. M. Ismail. "Cell surface changes during aging of human bladder epithelium examined by lectin probes and SEM." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 3 (1990): 966–67. http://dx.doi.org/10.1017/s0424820100162429.

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Ultrastructural changes have been shown to occur in the urinary bladder epithelium (urothelium) during the life span of humans. With increasing age, the luminal surface becomes more flexible and develops simple microvilli-like processes. Furthermore, the specialised asymmetric structure of the luminal plasma membrane is relatively more prominent in the young than in the elderly. The nature of the changes at the luminal surface is now explored by lectin-mediated adsorption visualised by scanning electron microscopy (SEM).Samples of young adult (21-31 y old) and elderly (58-82 y old) urothelia w

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Liu, Keyang, Zhanbo Wen, Na Li, et al. "Impact of Relative Humidity and Collection Media on Mycobacteriophage D29 Aerosol." Applied and Environmental Microbiology 78, no. 5 (2011): 1466–72. http://dx.doi.org/10.1128/aem.06610-11.

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ABSTRACTThis study was conducted to evaluate the effect of aerosol generation, methods of sampling, storage conditions, and relative humidity on the culturability of the mycobacteriophage D29. The lytic phage D29 can killMycobacterium tuberculosis, and the phage aerosol can be treated as a potential tool for tuberculosis treatment. The culturability of D29 was tested using a test chamber designed for the bioaerosols research against three spray liquids (deionized water, phosphate-buffered saline [PBS], and normal saline), four collection media (suspension medium [SM], nutrient broth, PBS, and

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Râpă, Maria, Cătălin Zaharia, Paul Octavian Stănescu, et al. "In Vitro Degradation of PHB/Bacterial Cellulose Biocomposite Scaffolds." International Journal of Polymer Science 2021 (October 6, 2021): 1–8. http://dx.doi.org/10.1155/2021/3820364.

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The present work reported the preparation of biocomposites based on poly(3-hydroxybutyrate) (PHB), plasticizer, and bacterial cellulose (BC) by melt processing and their testing by means of thermal properties (DSC), water absorption, and in vitro degradation. The surface of the biocomposites was analyzed via atomic force microscopy (AFM). In vitro degradation of the biocomposites was evaluated by weight loss and thermal properties (DSC) assessment after the immersion of the specimens in phosphate-buffered saline solution (PBS of pH 7.4) over 20 days. The results showed that the BC can reduce t

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Nemzer, Louis R., Marisa McCaffrey, and Arthur J. Epstein. "Enzyme entrapment in polyaniline films observed via fluorescence anisotropy and antiquenching." Modern Physics Letters B 28, no. 11 (2014): 1430004. http://dx.doi.org/10.1142/s021798491430004x.

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The facile entrapment of oxidoreductase enzymes within polyaniline polymer films by inducing hydrophobic collapse using phosphate buffered saline (PBS) has been shown to be a cost-effective method for fabricating organic biosensors. Here, we use fluorescence anisotropy measurements to verify enzyme immobilization and subsequent electron donation to the polymer matrix, both prerequisites for an effective biosensor. Specifically, we measure a three order of magnitude decrease in the ratio of the fluorescence to rotational lifetimes. In addition, the observed fluorescence antiquenching supports t

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LIU, PENGBO, LEE-ANN JAYKUS, ESTHER WONG, and CHRISTINE MOE. "Persistence of Norwalk Virus, Male-Specific Coliphage, and Escherichia coli on Stainless Steel Coupons and in Phosphate-Buffered Saline." Journal of Food Protection 75, no. 12 (2012): 2151–57. http://dx.doi.org/10.4315/0362-028x.jfp-12-197.

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Human noroviruses (NoVs) are a leading cause of acute gastroenteritis and are frequently transmitted by contaminated food, water, hands, and environmental surfaces. Little is known about their environmental stability and/or which alternative microorganisms can serve as effective surrogates. To examine whether Escherichia coli and male-specific coliphage MS2 can be appropriate surrogates for NoVs, approximately 6.8 log genomic equivalent copies of Norwalk virus (NV), and 6.0 to 6.5 log PFU or CFU of MS2 and E. coli, respectively, were inoculated onto stainless steel coupons and held at 4°C, roo

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Khatun, F., N. Begum, S. Akter, and MMH Mondal. "In vitro study of environmental and nutritional factors on the hatching and development of eggs of Haemonchus contortus." Bangladesh Veterinarian 30, no. 1 (2013): 1–9. http://dx.doi.org/10.3329/bvet.v30i1.16279.

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To determine the in vitro effects of environmental and nutritional factors on the development and hatching of eggs of Haemonchus contortus, eggs were cultivated at different temperatures, humidity and pH, in phosphate buffered saline (PBS), tap water, or normal saline, with and without faecal material and soil. No development of eggs occurred at 4ºC up to 15 days. Hatching was maximum (40.7%) at 30ºC on Day 5. Maximum hatching (39.5%) was observed on Day 6 at pH 6 and hatching was minimum (3.3%) on Day 4 at pH 3. Hatching did not occur at pH 2. At a relative humidity of 80 - 90%, maximum hatch

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Strapáč, I., Z. Bedlovičová, and M. Baranová. "Proteolytic Activity of Edible Spruce Morchella esculenta." Folia Veterinaria 63, no. 2 (2019): 60–64. http://dx.doi.org/10.2478/fv-2019-0020.

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Abstract This study focused on the determination of non-specific proteolytic activity of edible spruce Morchella esculenta in water extract, phosphate-buffered saline (PBS) solution (pH = 7.5) extract and a suspension prepared from 200 mg DW (dry weight) of edible spruce in PBS solution (pH 7.5). A clear casein solution was used as a substrate. The absorbances were measured in quartz cuvettes at the wavelength of 280 nm against a blank with zero concentration of trypsin. Non-specific proteolytic activity was expressed as trypsin equivalents per kilogram of mushroom dry weight (mg.kg−1 DW). All

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Moraes, Thais Gomes de, Alan Silva de Menezes, Renata Grazziotin-Soares, et al. "Impact of Immersion Media on Physical Properties and Bioactivity of Epoxy Resin-Based and Bioceramic Endodontic Sealers." Polymers 14, no. 4 (2022): 729. http://dx.doi.org/10.3390/polym14040729.

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This study assessed the effects of immersion media [distilled water (dw), phosphate buffered saline (pbs) and simulated body fluid (sbf)] in the physical properties [fluid uptake/sorption/solubility and alkalinization activity (pH)] and bioactivity of a bioceramic sealer: the BioRoot RCS (BioRoot) (Septodont). The epoxy-resin sealer AH Plus (Dentsply) was used as comparison. Sealers were immersed in dw, pbs and sbf to evaluate the fluid uptake/sorption/solubility and pH’s media. Bioactivity was assessed with SEM/EDS, FTIR-ATR and XRD. BioRoot solubility was as follows: sbf > pbs = dw. BioRo

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Skvortsov, Alexey, Ekaterina Babich, Alexey Redkov, Andrey Lipovskii, and Valentina Zhurikhina. "Stable in Biocompatible Buffers Silver Nanoisland Films for SERS." Biosensors 11, no. 11 (2021): 448. http://dx.doi.org/10.3390/bios11110448.

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We investigated the stability of silver nanoisland films, which were formed on glass surface by the method of out-diffusion, in biocompatible buffers and the applicability of the films in surface enhanced Raman scattering (SERS). We have shown that silver nanoisland films are stable in one of the most widespread in biological studies buffer—phosphate buffer saline (PBS), and in 1:100 water-diluted PBS, in the PBS-based buffer, in which NaCl is replaced by the same amount of NaClO4, and in acidic phosphate buffer. At the same time, the replacement of NaCl in PBS by N(CH3)4Cl leads to the degrad

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Szathmáry, Susan, Nandani Rajapakse, Ibolya Székely, et al. "Binding of mycoplasmas to solid phase adsorbents." Acta Veterinaria Hungarica 53, no. 3 (2005): 299–307. http://dx.doi.org/10.1556/avet.53.2005.3.3.

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The capture of mycoplasmas (M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticum and M. arthritidis) based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, a

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Al balawi, Aisha Nawaf, Nor Azah Yusof, Sazlinda Kamaruzaman, Faruq Mohammad, Helmi Wasoh, and Hamad A. Al-Lohedan. "High-Efficiency DNA Extraction Using Poly(4,4′-Cyclohexylidene Bisphenol Oxalate)-Modified Microcrystalline Cellulose-Magnetite Composite." International Journal of Polymer Science 2019 (June 4, 2019): 1–10. http://dx.doi.org/10.1155/2019/5738613.

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In this study, we studied the DNA extraction capability of poly(4,4′-cyclohexylidene bisphenol oxalate) following the surface modification and composite formation with that of microcrystalline cellulose (MCC) and magnetic iron oxide nanoparticles (NPs). The physical characterization techniques like scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, energy-dispersive X-ray analysis (EDX), and thermogravimetric analysis (TGA) were employed for the poly(bisphenol Z oxalate)-MCC-magnetite composite during different stages of its formation. The results confirmed the

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Ren, Long You, Wan Qian Guo, and Nan Qi Ren. "Influence Factors Analysis of Chloronitrobenzene Degradation in Biocatalyzed Electrolysis Systems." Applied Mechanics and Materials 448-453 (October 2013): 513–16. http://dx.doi.org/10.4028/www.scientific.net/amm.448-453.513.

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Chlorinated nitroaromatie compounds, which are widely used for synthesis industry of chemicals, pharmacy and pesticide, are listed on the priority pollutants by EPA and Chinese MEP. In this study, we investigated biocatalyzed electrolysis systems (BESs) with a bio-cathode seeding with enriched inoculum for the degradation of a typical chlorinated nitroaromatie compound: para-Chloronitrobenzene (4-CNB). Batch test results showed that the 4-CNB (30mg/L) would be completely degraded within HRT of 30 hours with 0.5V power supplied and NaAc (1g/L) as sole carbon source. The optimum operation condit

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Lau, Bernhard, Dietmar Becher, and Martin Hessling. "High Intensity Violet Light (405 nm) Inactivates Coronaviruses in Phosphate Buffered Saline (PBS) and on Surfaces." Photonics 8, no. 10 (2021): 414. http://dx.doi.org/10.3390/photonics8100414.

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It has been proven that visible light with a wavelength of about 405 nm exhibits an antimicrobial effect on bacteria and fungi if the irradiation doses are high enough. Hence, the question arises as to whether this violet light would also be suitable to inactivate SARS-CoV-2 coronaviruses. Therefore, a high-intensity light source was developed and applied to irradiate bovine coronaviruses (BCoV), which are employed as SARS-CoV-2 surrogates for safety reasons. Irradiation is performed in virus solutions diluted with phosphate buffered saline and on steel surfaces. Significant virus reduction by

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