Academic literature on the topic 'Phosphate solubilizer'
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Journal articles on the topic "Phosphate solubilizer"
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Nugroho, Sutopo Ghani, Dermiyati, Jamalam Lumbanraja, Sugeng Triyono, and Hanung Ismono. "Inoculation Effect of N2-Fixer and P-Solubilizer into a Mixture of Fresh Manure and Phosphate Rock Formulated as Organonitrofos Fertilizer on Bacterial and Fungal Populations." JOURNAL OF TROPICAL SOILS 18, no. 1 (2013): 75. http://dx.doi.org/10.5400/jts.2013.v18i1.75-80.
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Microbial N2-fixer and P-solubilizer were innoculated in a mixture of fresh manure and phosphate rock formulated as an Organonitrophos fertilizer. The population dynamics of bacteria and fungi growing during the composting process were observed. The inoculation treatments consisted of: K = mixture of 20% phosphate rock and 80% of fresh manure + decomposers (control), N = mixture of 20% phosphate rock and 80% of fresh manure + decomposers + N2-fixer (Azotobacter and Azospirillum sp.) , P = mixture of 20% phosphate rock and 80% of fresh manure + decomposers + P-solubilizer (A. niger and P. fluorescens), and NP = mixture of 20% phosphate rock and 80% of fresh manure + decomposers + N2-fixer + P-solubilizer. The results showed that inoculation of microbial N2-fixer and combination inoculation of N2-fixer and P-solubilizer increased the total bacterial population compared to that of the control as well as the only inoculation of microbial P-solubilizer on the 14th day of observation in which the bacteria reached the highest population. On all the observation days, the population of fungi in the inoculation of microbial P-solubilizer treatment increased significantly compared to that of the control. However, there was no difference between the populations of fungi in the inoculation of N2-fixer and combination inoculation of N2-fixer and Psolubilizer. The genus of fungy identified in the compost of the mixture of fresh manure and phosphate rock were Chytridium sp., Aspergillus sp., Rhizopus sp., and Fusarium sp.[How to Cite : Nugroho SG, Dermiyati, J Lumbanraja, S Triyono, H Ismono. 2013. Inoculation Effect of N2-Fixer and P-Solubilizer into a Mixture of Fresh Manure and Phosphate Rock Formulated as Organonitrofos Fertilizer on Bacterial and Fungal Populations. J Trop Soils, 18 (1): 75-80. doi: 10.5400/jts.2013.18.1.75][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.1.75]
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Narsian, Varsha, and H. H. Patel. "Aspergillus aculeatus as a rock phosphate solubilizer." Soil Biology and Biochemistry 32, no. 4 (2000): 559–65. http://dx.doi.org/10.1016/s0038-0717(99)00184-4.
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Baishya, Ratul, and Rhituporna Saikia. "Phosphate Solubilizing Bacteria in Certain Agricultural Crop Soils of Delhi." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 4, no. 01 (2018): 70–75. http://dx.doi.org/10.18811/ijpen.v4i01.11613.
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Phosphate solubilizing bacteria (PSB) helps in the solubilization of insoluble phosphates and thus lead to increase in crop yields. A study was conducted to isolate and characterize biochemically PSB from different agricultural crop soils of Delhi such as Garlic, Radish, Chilli, Onion and Cabbage. PSB were isolated in Pikovskaya solid medium and formation of solubilization (halo) zone was measured. 16 PSB were isolated and identified. The selected PSB differed in phosphate solubilizing efficiency, production of organic acids and phosphatases. Citrobacter sp. and Pseudomonas sp. were dominant in all the crop plants. Among all the isolates, Pseudomonas sp. proved to be an efficient phosphate solubilizer.
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Singh, Mahendra. "Isolation and characterization of insoluble inorganic phosphate solubilizer rice rhizosphere strain Enterobacter cloacae BAU3." Journal of Applied and Natural Science 10, no. 4 (2018): 1204–9. http://dx.doi.org/10.31018/jans.v10i4.1929.
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The objective of the present study was to isolate and characterize most efficient phosphate solubilizing bacteria (PSB) from rice rhizosphere. The study was carried out during the Kharif season’2018 at Department of Soil Science and Agricultural Chemistry, Bihar Agricultural University, Sabour, Bhagalpur, Bihar. The availability of phosphorous to plants for uptake and utilization is limited in soil due to fixation in the form of Fe-P, Al-P and Ca-P. The use of phosphate solubilizing bacteria can prove to be helpful measure to supply phosphorous to the crops to increase the productivity. In the present investigation, a total of 10 isolates were obtained from rice rhizosphere soil samples. All ten isolated isolates were shown phosphorus solubilization. Out of ten isolates BAU3 was found to be most potent phosphate solubilizers showing clear halo zone around its colony. The isolate BAU3 showed 20.00 mm phosphate solubilizing halo zone around its colony. The solubilization index (SI) of the isolate BAU3 was also calculated at the end of the incubation period and observed phosphate solubilization index (SI) of 3.22. The isolate BAU3 showed maximum insoluble phosphate solubilization of 450.24 ?g ml-1 and isolates BAU3 was selected for subsequent studies. The bacterial isolates BAU3 was gram negative, non-spore forming rods shaped. On the basis of the 16SrDNA sequencing, isolate BAU3 was identified as Enterobacter cloacae strain BAU3 (Genebank Accession No. MK033472). The isolated strain of bacterial has potential to solubilize insoluble phosphorus and it can be utilized for preparation of microbial inoculants or biofertilizers.
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YAÑEZ-OCAMPO, GUSTAVO, MARTHA E. MORA-HERRERA, ARNOLDO WONG-VILLARREAL, et al. "Isolated Phosphate-Solubilizing Soil Bacteria Promotes In vitro Growth of Solanum tuberosum L." Polish Journal of Microbiology 69, no. 3 (2020): 357–65. http://dx.doi.org/10.33073/pjm-2020-039.
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The capacity of four bacterial strains isolated from productive soil potato fields to solubilize tricalcium phosphate on Pikovskaya agar or in a liquid medium was evaluated. A bacterial strain was selected to evaluate in vitro capacity of plant-growth promotion on Solanum tuberosum L. culture. Bacterial strain A3 showed the highest value of phosphate solubilization, reaching a 20 mm-diameter halo and a concentration of 350 mg/l on agar and in a liquid medium, respectively. Bacterial strain A3 was identified by 16S rDNA analysis as Bacillus pumilus with 98% identity; therefore, it is the first report for Bacillus pumilus as phosphate solubilizer. Plant-growth promotion assayed by in vitro culture of potato microplants showed that the addition of bacterial strain A3 increased root and stems length after 28 days. It significantly increased stem length by 79.3%, and duplicated the fresh weight of control microplants. In this paper, results reported regarding phosphorus solubilization and growth promotion under in vitro conditions represent a step forward in the use of innocuous bacterial strain biofertilizer on potato field cultures.
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Mutalib, Asilah Abdul. "EFFECTS OF PHOSPHATE SOLUBILIZERS AND BIOCHAR ON GROWTH AND YIELD OF TOMATO (Solanum lycopersicum)." Journal of Vocational Education Studies 2, no. 2 (2019): 67. http://dx.doi.org/10.12928/joves.v2i2.1203.
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A study was conducted to determine the effects of phosphate solubilizers and biochar on growth and yield of tomato plants. For this purpose, a 4×3 factorial experiment based on Completely Randomized Block Design (RCBD) with 4 replications were conducted in the growth room. In this factorial experiment, 4 levels of phosphate solubilizer bacteria (B1, B2, Bmix and Control) and 3 levels of biochar (Powder, Large Size, and Control) were applied on tomato plants to identify changes in growth parameters and yield. Results were analysed using Genstat Statistical Package (16th Edition). Analysis of Variance (ANOVA) were conducted to test the effects of each factor and their interactions. Subsequently, Tukey test was used to compare the treatments means at P<0.05. The results showed that phosphate solubilizers had no significant effects on the growth parameters measured, while biochar treatments significantly affected the total fresh weight of the tomatoes and increased the speed of plant growth in tomatoes (Flowering and fruiting). In addition, there was no interaction between bacterial inoculation and biochar application on the parameters studied. The bacterial inoculation did not significantly affect any parameters studies. This is contrasted by the results from the treatments with biochar application which showed the enhancement of plant growth and yield. The sustainability of the bacterial strains used are suggested to be tested before starting the experiment.
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Lapeyrie, F., J. Ranger, and D. Vairelles. "Phosphate-solubilizing activity of ectomycorrhizal fungi in vitro." Canadian Journal of Botany 69, no. 2 (1991): 342–46. http://dx.doi.org/10.1139/b91-046.
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An easy to use method is presented here to compare and study the mineral phosphate-solubilizing activity of ectomycorrhizal fungi. This technique can discriminate between strains with differing phosphate-solubilizing activities. Synthetic mineral phosphates, crystalline or amorphous, were differentially solubilized by 10 ectomycorrhizal fungi. Natural crystalline phosphates studied do not seem to be solubilized by fungi under similar experimental conditions. Paxillus involutus 1 appears to be able to solubilize calcium phosphates using either ammonium or nitrate nitrogen, but the other isolates were able to effectively solubilize phosphate only in the presence of ammonium. This has implications regarding the possible mechanism used to solubilize phosphate by these isolates. Recrystallization can be seen in the culture medium if calcium ions are present. The type of crystals depends on the phosphate source and on the fungal strain. This technique is suitable for screening a large number of ectomycorrhizal strains. The significance of phosphate-solubilizing activity to plant growth stimulation needs to be determined by field trials. Key words: ectomycorrhizal fungus, phosphate, solubilization.
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Yadav, Anurag, Kusum Yadav, and Anupam Vashistha. "Phosphate solubilizing activity of Pseudomonas fluorescens PSM1 isolated from wheat rhizosphere." Journal of Applied and Natural Science 8, no. 1 (2016): 93–96. http://dx.doi.org/10.31018/jans.v8i1.754.
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A study was designed to screen and analyze the efficient phosphate solubilizing bacteria (PSBs) from wheat rhizosphere. Five biovars of Pseudomonas fluorescens (PSM1, PSM2, PSM3, PSM4 and PSM5) were isolated from wheat rhizosphere and Bacillus megaterium MTCC 8755 procured from microbial type culture collection (MTCC) Chandigarh, India. The P. fluorescens biovar PSM1 was observed to be most efficient phosphate solubilizer. Inoculation of P. fluorescens PSM1 and B. megaterium MTCC 8755, alone and in combination, caused the highest phosphate solubilization at pH 5. At this pH, maximum phosphate solubilization was observed with B. megaterium MTCC 8755 inoculation (8.2 mg mL-1) on sixth day of incubation with P. fluorescens PSM1 (8 mg mL-1) on seventh day of incubation and with dual bacterial treatment (10.5 mg mL-1) on the fourth day of incubation. A correlation coefficient of linear regression equation of phosphate solubilization with pH indicated that pH value of the medium was directly correlated with tricalcium phosphate solubilization. The study will help in choosing soil pH specific PSB inoculant for optimizing plant growth.
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Oktaviani, Eka, Arina Tri Lunggani, and Rejeki Siti Ferniah. "Karakter Rhizobakteri Pelarut Fosfat Potensial dari Rhizosfer Tumbuhan Mangrove Teluk Awur Kabupaten Jepara secara Mikrobiologi." Jurnal Ilmu Lingkungan 18, no. 1 (2020): 58–66. http://dx.doi.org/10.14710/jil.18.1.58-66.
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Ekosistem mangrove terdiri dari komponen penyusun komunitas mangrove yang berinteraksi dengan faktor lingkungan di habitat tersebut. Ekosistem ini mudah mengalami kerusakan akibat kondisi pasang naik dan pasang surut wilayah perairan laut maupun hilir sungai, yang bermuara ke perairan laut. Peran penting ekosistem ini adalah dalam perlindungan daerah pesisir karena mampu melawan kerusakan yang disebabkan oleh perairan laut, seperti tsunami dan badai siklon tropis. Akan tetapi, ekosistem ini sering tidak diperhatikan atas fungsi tersebut. Ekosistem ini dilindungi di dunia karena memiliki tingkat kerusakan komponen penyusun yang tinggi. Ekosistem mangrove Teluk Awur yang terletak di Kabupaten Jepara, Provinsi Jawa Tengah, merupakan salah satu ekosistem mangrove yang mengalami kerusakan cukup parah karena perluasan lahan budidaya ikan (tambak), sehingga mendorong terjadinya erosi pantai. Peremajaan kembali dan konservasi ekosistem mangrove dapat dilakukan dengan menggunakan bakteri tanah yang mampu mendukung pertumbuhan tanaman atau yang biasa disebut Plant Growth Promoting Rhizobacteria (PGPR). Salah satu mekanisme pendukung pertumbuhan tanaman oleh kelompok PGPR dapat dilakukan dengan aktivitas pelarutan fosfat karena fosfat dalam tanah berada dalam bentuk senyawa yang sulit diserap oleh tanaman. Penelitian ini bertujuan untuk memperoleh isolat Rhizobakteri pelarut fosfat yang unggul dalam melarutkan fosfat secara in-vitro dan mengetahui karakter isolat yang diperoleh. Isolasi dan penapisan rhizobakteri pelarut fosfat dilakukan menggunakan medium Pikovskaya agar. Karakterisasi isolat potensial secara mikrobiologi dan uji aktivitas biokimia. Hasil penelitian menunjukkan bahwa rhizobakteri pelarut fosfat potensial yang berhasil diisolasi, secara mikrobiologi teridentifikasi ke dalam genus Enterobacter. Mangrove ecosystems consist of constituent components of mangrove communities that interact with environmental factors in the habitat. This ecosystem is susceptible to damage due to the high tide and tidal conditions of the sea and downstream rivers that flow into the ocean waters. The important role of this ecosystem is in the protection of coastal areas because it is able to resist damage caused by marine waters such as tsunamis and tropical cyclone storms. However, this ecosystem is often overlooked to these functions. This ecosystem is protected in the world because it has a high level of damage to its constituent components. Mangrove ecosystem of Teluk Awur, which is located in Jepara Regency, is one of the mangrove ecosystems that suffered severe damage due to the expansion of fish farming land (ponds). Rejuvenation and conservation of mangrove ecosystems can be done by using bacteria that support mangrove plant growth, which commonly called Plant Growth Promoting Rhizobacteria (PGPR). One of the supporting mechanisms of plant growth by the PGPR group can be carried out with phosphate dissolving activity because phosphate in the soil is in the form of compounds that are difficult to absorb by plants. This study aims to obtain potential phosphate solubilizer Rhizobacteria in dissolving phosphate in-vitro and to determine character of the obtained isolate. Isolation and screening of potential phosphate solubilizer Rhizobacteria were carried out using Pikovskaya agar medium. Microbiological characterization of potential isolates was carried out based on microbiological and/or biochemical activity. The results showed that potential phosphate solubilizer Rhizobacteria, microbiologically identified to genus of Enterobacter.
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Schiavo, Jolimar Antonio, Jader Galba Busato, Marco Antonio Martins, and Luciano Pasqualoto Canellas. "Recovery of degraded areas revegeted with Acacia mangium and Eucalyptus with special reference to organic matter humification." Scientia Agricola 66, no. 3 (2009): 353–60. http://dx.doi.org/10.1590/s0103-90162009000300010.
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Humidified fractions of organic matter and soil microorganism populations are used as environmental quality indicators. This work aimed to study the changes in chemical and microbiological soil attributes, as well as in the humidified fractions, of the organic matter in a substrate from a clay extraction area cropped with Brachiaria mutica, Acacia mangium and Eucalyptus. In the Eucalyptus area, the P contents increased linearly with planting time. However, only at the twelfth year, differences between Eucalyptus and B. mutica areas have occurred. In the A. mangium area, such differences in the P content occurred at the third year with increment of 43%, at the 0-10 cm layer, in relation to B. mutica. Also, at the 0-10 cm layer, the total carbon contents were 98%, 78%, 70% and 40% higher than those found in Eucalyptus with three, five, twelve years of age and in the B. mutica area, respectively. Such increments also occurred in the humidified fractions, especially in the fulvic acids (C FA). The population of microorganisms was higher in the A. mangium area, mainly in the summer, where it was observed a positively correlation with total carbon (total bacteria, r = 0.96**, total fungi, r = 0.91*, and phosphate solubilizer microorganisms, r = 0.98**) and with the C FA fraction (total bacteria r = 0.96**, total fungi, r = 0.90*, and phosphate solubilizer microorganisms, r = 0.98**). The use of A. mangium led to improvements in the chemical and microbiological soil attributes in the substrate
Dissertations / Theses on the topic "Phosphate solubilizer"
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Oliveira, Jéssica Alves de. "Estabelecimento de bananeira (Musa sp.) em solo degradado /." Ilha Solteira, 2018. http://hdl.handle.net/11449/154511.
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Orientador: Ana Maria Rodrigues Cassiolato<br>Resumo: O solo degradado em estudo originou-se pela retirada da sua camada superficial, que compreende a vegetação, a matéria orgânica, os nutrientes e os microrganismos, cujos processos são essenciais para o solo ser considerado produtivo. Sua recuperação é importante para manter o equilíbrio da biodiversidade de flora e fauna e protege-lo contra a erosão, entre outros fatores. O objetivo deste trabalho foi o condicionamento do subsolo pela adição de cinza de biomassa de cana-de-açúcar, da inoculação de fungo micorrízico arbuscular (FMA – Glomus clarum) e da inoculação de fungos e de bactéria solubilizadores de fosfato, buscando uma técnica para a recuperação de solos degradados. A bananeira (Musa sp. cv. Mysore) foi utilizada como planta indicadora para as modificações que ocorreram no solo. O delineamento experimental foi em blocos casualizados, em esquema fatorial 2 x 2 x 3 + 2, com 4 repetições por tratamento, totalizando 12 tratamentos, com 4 repetições (bloco). O primeiro fator foi com e sem inoculação de FMA, o segundo foi com e sem adição de cinza proveniente da queima do bagaço de cana-de-açúcar, o terceiro foi com e sem inoculação de fungos e de bactéria solubilizadores de fosfato, e dois tratamentos adicionais, área conservada de Cerrado (SCRC) e área degradada sem intervenção (ADSI). Após 67 e 274 dias do plantio foram avaliados os atributos químicos e microbiológicos do solo na camada de 0,0 a 0,10 m, além da altura, do diâmetro, do índice relativo de clorofila e do P t... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: The degraded soil under study originated by the removal of the superficial layer, which includes vegetation, organic matter, nutrients and microorganisms, whose processes are essential for the soil to be considered productive. Its recovery is important to maintain the balance of biodiversity of flora and fauna and protect it against erosion, among other factors. The objective of this work was the subsoil conditioning by the addition of sugarcane biomass ash, the inoculation of arbuscular mycorrhizal fungi (AMF - Glomus clarum) and the inoculation of fungi and bacteria phosphate solubilizers, searching for a technique for recovery of degraded soils. The banana plants (Musa sp. cv. Mysore) was used as an indicator plant for the changes that occurred in the soil. The experimental design was a randomized complete block design, with a 2 x 2 x 3 +2 factorial scheme, with 4 replicates per treatment, in a total of 12 treatments, with 4 replicates (block). The first factor was with and without AMF inoculation, the second was with and without ash added from the burning of sugarcane bagasse, the third was with and without inoculation of fungi and bacteria phosphate solubilizers and two controls area, Cerrado conservation area (CCAR) and degraded area without intervention (DAWI). After 67 and 274 days of planting, the soil chemical and microbiological attributes were evaluated in the 0.0 to 0.10 m layer, as well as the height, diameter, relative chlorophyll index, and total leaf P. The a... (Complete abstract click electronic access below)<br>Mestre
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Silva, Ubiana de Cássia. "Seleção e caracterização de mutantes de Aspergillus niger eficientes em solubilizar fosfato na presença de fluoreto." Universidade Federal de Viçosa, 2013. http://locus.ufv.br/handle/123456789/5361.
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Previous issue date: 2013-08-30<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The use P solubilizing microorganisms are an alternative for a sustainable use of P against a backdrop of depletion of high-grade ores. Nevertheless, the chemical characteristics of rock phosphates (RP), particularly the level of toxic elements, can affect the efficiency of microbial solubilization. Fluoride (F-) released from fluorapatites has been shown to significantly inhibit P solubilization by Aspergillus niger, stimulating the search for alternatives to overcome F- toxicity. Thus, the aim of this study was to select A. niger mutants efficient at P solubilization in the presence of F-. Three selected mutants were also characterized as to their P solubilization mechanisms and the solubilization potential of different P sources. Twenty-nine mutants were obtained that presented changes in their phosphate solubilization activity in comparison to the wild type (WT). The mutant FS1-331 showed higher solubilization of Araxá RP, while FS1-555 promoted higher soluble P when grown in media with calcium phosphate supplemented with F- and in those with pure P sources. These mutants also showed higher tolerance to F- than the WT and displayed changes in the production of organic acids. The higher production of oxalic acid by FS1-331 and FS1-555 correlated with their improved capacity of Araxá RP solubilization. A mutant with decreased P solubilization capacity showed lower production of organic acids, corroborating the importance of these compounds for RP solubilization. FS1-331 was more efficient at solubilizing Araxá, Catalão, and Patos de Minas RPs than the WT and FS1-555. The variation in P solubilization capacity of the mutants obtained in this work may help clarify the RP solubilization mechanisms by A. niger. Moreover, the mutants with better performance selected in this work show potential for use in microbial RP solubilization systems with P sources rich in fluoride.<br>O uso dos micro-organismos solubilizadores de fosfato (P) é uma alternativa para o uso sustentável do P tendo em vista a diminuição das reservas fosfáticas de alta qualidade. As características químicas dos fosfatos de rocha (FR), em especial o nível de elementos tóxicos, podem influenciar a eficiência de solubilização microbiana. O fluoreto (F-) liberado de fluorapatitas inibi significativamente o processo de solubilização por Aspergillus niger FS1. Assim, o objetivo deste estudo foi selecionar mutantes de A. niger eficientes na solubilização de fosfato na presença de F-. Três mutantes selecionados foram também caracterizados quanto aos mecanismos de solubilização de fosfato e ao potencial de solubilização de diferentes fontes de P. Vinte e nove mutantes com alterações na solubilização de fosfato foram obtidos. O mutante FS1-331 demonstrou maior potencial de solubilização de fosfato de Araxá, enquanto que o mutante FS1-555 aumentou o P solubilizado em meio de cultura com fosfato de cálcio suplementado com F- e naqueles com fontes sintéticas de P. Os mutantes avaliados mostraram maior tolerância ao F- do que o tipo selavagem e tiveram a produção de ácidos orgânicos alterada. A maior produção de ácido oxálico pelos mutantes FS1-331 e FS1-555 relacionou-se com o melhor desempenho dessas estiprpes na solubilização de fosfato de Araxá. Um mutante com solubilização de P diminuída (FS1-375) apresentou menor produção de ácidos orgânicos quando comparado ao tipo selvagem, corroborando a importância desses compostos para o processo de solubilização de FRs. O mutante FS1-331 foi o mais eficiente na solubilização dos FRs de Araxá, Catalão e Patos de Minas. A variação na solubilização de P das estirpes fúngicas estudadas pode contribuir para a melhor compreensão do processo de solubilização de fosfatos por A. niger. Além disso, os mutantes mais eficientes obtidos apresentam alto potencial de aplicação em sistemas microbianos de solubilização de fosfatos com fontes de P ricas em fluoreto.
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Baglan, Nicolas. "Contribution a l'etude de la solubilite de l'orthophosphate de thorium dans des milieux proches de la neutralite, en presence ou non d'anions phosphates." Paris 11, 1992. http://www.theses.fr/1992PA112437.
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Une partie du travail a consiste a mettre au point la synthese de l'orthophosphate de thorium. Deux formes cristallographiques ont ete mises en evidence, suivant la temperature de fin de synthese. Pour faciliter le marquage de l'orthophosphate de thorium, forme haute temperature par le thorium 227, celui-ci a ete prepare par voie humide, a partir d'un melange de nitrate de thorium et d'acide phosphorique. La non evolution de sa structure, apres des mois d'immersion en solution, a ete verifiee aux rayons x. Le potentiel zeta de l'orthophosphate de thorium a ete mesure dans diverses solutions. Il depend essentiellement du ph. Les experiences effectuees avec des solutions marquees par le thorium 227 et un solide inactif, ont permis de maitriser un certain nombre de parametres intervenant dans l'equilibre solide-solution et de mettre au point la procedure experimentale pour separer correctement les phases. Les mesures ont montre que l'orthophosphate de thorium est trop insoluble dans les milieux neutres non complexants pour permettre de mesurer aisement la concentration du thorium de la solution en equilibre. Seule une valeur limite superieure de sa concentration dans l'eau peut etre proposee, soit 3. 10##1#1 m. Pour les milieux tres acides, un produit de solubilite a pu etre calcule. Sa valeur est tres inferieure a celle proposee habituellement, et si faible que l'on peut s'interroger sur sa signification. En milieux phosphoriques, il a ete possible de deduire la forme la plus probable du complexe predominant, a ph voisin de 7 et pour des concentrations en anions phosphates comprises entre 0,1 et 1 m
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Dias, Anelise. "Caracteriza??o e sele??o de bact?rias fluorescentes promotoras do crescimento de couve." Universidade Federal Rural do Rio de Janeiro, 2011. https://tede.ufrrj.br/jspui/handle/jspui/1162.
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Previous issue date: 2011-09-23<br>Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES<br>A collection of 189 bacterial isolates, obtained from organic system of vegetable production,
was characterized aiming to selection of cabbage growth promoting strains. Isolatings were
performed from rhizosphere of vegetables and soil after harvest of cultivated species. The vast
majority of the strains showed biochemical profiles identical to Pseudomonas (fluorescent
group). While profiles identical to Burkholderia, Stenotrophomonas and Acinetobacter
constituted other groups of representatives. Characterization of isolates took into account the
capacity for synthesis of compounds possibly involved in mechanisms to stimulate plant
growth, namely: biofilms, siderophores, acyl homoserine lactones (AHLs), indole-3-acetic
acid (IAA) and indole related compounds, as well as solubilization of calcium phosphate. A
total of 94 isolates were evaluated in the greenhouse for their ability to promote the growth of
cabbage (Brassica oleracea var. acephala) in pots containing unsterilized loam soil. In
addition, consortia were evaluated between components of the collection and rhizobia for the
same purpose. In addition, it compared the effects of application of certain producing IAA
isolates and the application of synthetic IAA on the growth of cabbage. Laboratory
experiments showed that in vitro, 100% of the isolates formed biofilm, 71% synthesized
siderophores, 31% produced AHLs, 95% produced IAA and 74% solubilized P. However,
bioassays in the greenhouse showed that only 12% of the isolates induced significant
responses to promote the growth of cabbage. Of these, a subgroup composed of 10 strains of
rhizobacteria given increments in growth parameters of both the biomass and the root system
of vegetables. Biofilms, siderophores, IAA and solubilization of P were common
characteristics of the subgroup. No synergistic effect was observed among isolates in
consortia, although it was found that the two strains of rhizobia consortium promoted the
growth of inoculated plants. The isolates R1132 and S311 were higher than the application of
synthetic IAA. The results suggested the potential of 12 bacteria to promote plant growth and
increase production of cabbage<br>Uma cole??o de 189 isolados bacterianos, provenientes de sistema org?nico de produ??o de
hortali?as, foi caracterizada com vistas ? sele??o de estirpes promotoras do crescimento de
couve. Os isolamentos foram realizados a partir da rizosfera de hortali?as e do solo ap?s
colheita das esp?cies cultivadas. A grande maioria das estirpes apresentou perfil bioqu?mico
id?ntico a Pseudomonas (grupo fluorescente). Enquanto perfis id?nticos a Burkholderia,
Acinetobacter e Stenotrophomonas constitu?ram outros grupos de representantes. A
caracteriza??o dos isolados levou em conta a capacidade de s?ntese de compostos
possivelmente envolvidos em mecanismos de est?mulo ao crescimento de plantas, a saber:
biofilmes, sider?foros, acil-lactonas homoserinas (ALHs), ?cido indol-3-ac?tico (AIA) e
compostos ind?licos relacionados, al?m da solubiliza??o de fosfato de c?lcio. Um total de 94
isolados foi testado na casa de vegeta??o quanto ? capacidade de promover o crescimento de
couve (Brassica oleraceae var. acephala) em vasos contendo solo distr?fico n?o esterilizado.
Paralelamente, foram avaliados cons?rcios entre componentes da cole??o e riz?bios para igual
finalidade. Em adendo, foram comparados os efeitos da aplica??o de determinados isolados
produtores de AIA e da aplica??o de AIA sint?tico sobre o crescimento da couve. Os
experimentos de laborat?rio indicaram que, in vitro, 100% dos isolados formaram biofilme,
71% sintetizaram sider?foros, 31% produziram AHLs, 95% produziram AIA e 74%
solubilizaram P. No entanto, os bioensaios na casa de vegeta??o evidenciaram que apenas
11% dos isolados bacterianos induziam respostas significativas de promo??o do crescimento
da couve. Desses, um subgrupo formado por 10 estirpes de rizobact?rias conferiu incrementos
nos par?metros de crescimento, tanto da biomassa a?rea quanto do sistema radicular da
hortali?a. Biofilmes, sider?foros, AIA e solubiliza??o do P foram caracter?sticas comuns do
subgrupo. N?o foi observado efeito sin?rgico entre os isolados em cons?rcios, embora tenha
sido verificado que as duas estirpes de riz?bios consorciadas promoveram o crescimento das
plantas inoculadas. Os isolados R1132 e S311 foram superiores ? aplica??o do AIA sint?tico.
Os resultados obtidos sinalizaram o potencial de 12 bact?rias que promover o crescimento das
plantas e incrementar a produ??o de couve.
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Dieudonne, Thibaud. "Functional and Structural Characterization of Lipid Flippases : The Yeast Drs2p/Cdc50p and the Disease-Related Human Atp8b1/Cdc50a Complexes Structure and Autoregulation of a P4-ATPase Lipid Flippase Screening of Detergents for Stabilization of Functional Membrane Proteins High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions Slow Phospholipid Exchange between a Detergent-Solubilized Membrane Protein and Lipid-Detergent Mixed Micelles: Brominated Phospholipids as Tools to Follow Its Kinetics." Thesis, université Paris-Saclay, 2020. http://www.theses.fr/2020UPASS023.
Full textAbstract:
Les cellules sont entourées de membranes lipidiques organisées en bicouche séparant ainsi le milieu intracellulaire du milieu extérieur. L’une des caractéristiques des cellules eucaryotes est de posséder une distribution asymétrique des lipides constituants les membranes de la voie sécrétoire. En effet, dans ces membranes, la phosphatidylcholine (PC) et les sphingolipides (SL) sont majoritairement retrouvés sur le feuillet externe alors que la phosphatidylsérine (PS) et la phosphatidyléthanolamine (PE) sont séquestrées sur le feuillet interne. Cette asymétrie est maintenue grâce à la présence de transporteurs de lipides. Parmi ces transporteurs, on retrouve les flippases, qui grâce à l’énergie apportée par la consommation d’ATP, transportent les lipides du feuillet interne vers le feuillet externe. Les flippases appartiennent à la superfamille des ATPases de type P et ont été reliées à différentes pathologies humaines lorsqu’elles sont mutées. Par exemple, des mutations du gène ATP8B1 sont responsables d’une forme de cholestase intrahépatique, une maladie hépatique sévère. Dans cette thèse, nous avons étudier le mécanisme de régulation de deux flippases : la flippase de levure PS spécifique Drs2p/Cdc50p ainsi que la flippase humaine ATP8B1/CDC50A. Les deux flippases ont été exprimées dans la levure de bière S. cerevisiae et purifiées afin de réaliser leur caractérisation fonctionnelle. Nos résultats montrent que les deux flippases sont régulées par des phosphoinositides et auto-inhibées par leurs extrémités N- et C-terminales<br>Living cells are surrounded by membranes organized in bilayers, separating the intracellular medium from the extracellular environment. A hallmark of eukaryotic membranes from the late secretory/endocytic pathways is the asymmetric distribution of phospholipids between the two leaflets. Indeed, phosphatidylcholine (PC) and sphingolipids (SL) are mainly found in the outer leaflet whereas phosphatidylserine (PS) and phosphatidylethanolamine (PE) are sequestered in the inner leaflet. This asymmetry is maintained thanks to different membrane lipid transporters. Among them, flippases, which are transporters fueled by ATP hydrolysis, translocate lipids from the outer to the inner leaflet. Flippases belong to the P4-ATPase family and have been linked to several diseases. For instance, mutated forms of a human P4-ATPase, ATP8B1, are responsible for intrahepatic cholestasis, a severe liver disease. In this thesis, we investigated the regulatory mechanism of two flippases, the yeast PS-specific flippase complex Drs2p/Cdc50p, and the human disease-related flippase complex ATP8B1/CDC50A. Both proteins were expressed in S. cerevisiae and purified for downstream functional characterization. Our results demonstrate that both flippases are tightly regulated by phosphoinositides and autoinhibited by their N- and C-terminal extensions
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Vann, Dustin Glen. "Evaluation of solubilized proteins as an alternative to phosphates for meat enhancement." 2006. http://digital.library.okstate.edu/etd/umi-okstate-1928.pdf.
Full textBook chapters on the topic "Phosphate solubilizer"
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Raghunandan, B. L. "Rhizobacterial Phosphate Solubilizers in Sustainable Agriculture: Concepts and Prospects." In Microorganisms for Sustainability. Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-6241-4_6.
Full textConference papers on the topic "Phosphate solubilizer"
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Moake, J. L., M. A. Harris, C. E. Whitley, and C. P. Alfrey. "RAPID, SENSITIVE N0N-RADI0ACTIVE QUANTIFICATION AND ANALYSIS OF PLASMA VON WILLEBRAND FACTOR (vWF) MULTIMERS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644085.
Full textAbstract:
Assessment of plasma vWF abnormalities by clinical coagulation laboratories is difficult because the available test systems for vWF antigen quantification and multimer analysis are expensive, laborious, and require days, radioactive anti-vWF antibodies and autoradiographic methods. We have devised simple, rapid, sensitive alternative techniques for vWF quantification and multimer analysis that can be readily installed in clinical laboratories. Plasma vWF antigen quantification is by a 2 hour enzyme immunoassay that accurately detects levels as low as 0.23% of normal. Plasma vWF to be quantified is bound to polyclonal monospecific antihuman vWF attached to small glass beads, and anti-human vWF conjugated with alkaline phosphatase is added to make an insoluble "sandwich." A substrate solution consisting of phenylphosphate and 4-amino-antipurine is added, followed by potassium ferricyanide. Optical density (at 490-510 nm) of the red color that develops is directly proportional to the plasma concentration of vWF antigen. Plasma vWF multimeric analysis is by a one-day electrophoretic immunobiot procedure. Plasma vWF multimer forms are solubilized in SDS-urea-Tris-EDTA, separated by horizontal 1% agarose gel electrophoresis, and transferred to a cationic membrane. Other protein binding sites on the membrane are blocked with milk proteins, and the membrane is overlaid with anti-vWF IgG linked to alkaline phosphatase. vWF multimers are then displayed as blue bands by soaking the membrane in an alkaline solution of the histochemical stain, fast blue RR (commonly used for leukocyte alkaline phosphatase scoring) dissolved in naphtol AS-MX phosphate. These simple, non-radioactive procedures performed together permit the rapid distinction of classical (Type I) von Willebrand's disease (vWD), characterized by low vWF antigen and normal multimers, from the Type II vWD syndromes, characterized by a relative deficiency of the largest plasma vWF forms. Unusually large vWF multimers, present in remission plasma of patients with chronic relapsing thrombotic thrombocytopenic purpura (TTP), are also easily detected using this rapid system of multimer analysis.
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PEERSCHKE, E. IB, and B. Ghebrehiwet. "CHARACTERIZATION OF HUMAN PLATELET ClQ BINDING SITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643503.
Full textAbstract:
We have recently shown that platelets possess specific binding sites for Clq, a subcomponent of the first component of complement, Cl, and that occupancy of these receptor sites correlates with the previously described inhibitory effect of Clq on collagen-induced platelet aggregation. To further characterize platelet Clq receptors, washed platelets were solubilized in 5 mM sodium phosphate buffer, pH 7.5 containing 10 mM EDTA, 150 mM NaCl, 10 mM EACA, 0.5 mM PMSF, and 1% Triton X-100. After dialysis against 5 mM phosphate buffer pH 7.5 containing 10 mM EDTA, 20 mM NaCl, 10 mM EACA,0.5 mM PMSF and 0.1% Triton X-100, the lysate was passed over Clq-Sepharose-4B affinity columns. A single protein peak eluted with buffer containing 300 mM NaCl. This peak was composed of two predominant molecular weight species (85-95K, 60-66K) as assessed by SDS-polyacrylamide gel electrophoresis under non-reducing conditions. When 125-iodine surface labeled platelets were solubilized and applied to Clq-Sepharose affinity resins, the same two molecular weight species eluted and could be visualized by autoradiography following SDS-polyacrylamide gel electrophoresis. Immunoabsorption studies performed under nondenaturing conditions using protein A and the IIl/Dl monoclonal antibody, which binds specifically to platelets and inhibits platelet-Clq interactions, revealed that the 85-95K molecular weight component was preferentially absorbed, but incomplete immunoabsorption of the 60-66K molecular weight constituent was also noted. Affinity purified Clq binding sites sedimented as a single peak during 5-40% sucrose density ultracentrifugation with an S value of approximately 2.4. In addition, both the 85-95K and the 60-66K molecular weight species coeluted in the void volume of Sephadex G-100 columns. The data suggest that the 85-95K and 60-66K molecular weight species represent platelet membrane Clq binding sites, and that these sites may form weak, noncovalently associated complexes.
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Van Haarlem, L. J. M., H. C. Hemker, B. A. M. Soute, and C. Vermeer. "GLA-CONTAINING PROTEINS FROM CALCIFIED HUMAN ATHEROSCLEROTIC PLAQUES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643747.
Full textAbstract:
Vitamin K-dependent carboxylase activity has been detected in human andbovine vessel wall. Studies comparingthe carboxylases from liver and vessel wall revealed that the enzyme systems may be regarded as isoenzymes withwidely different substrate specificities. The carboxylated product of vessel wall carboxylase has not yet been identified, but it seems plausible that it will be found amongst the Gla-containing proteins which are abundantly present in calcified atherosclerotic plaques (Gla= gammacarboxyglutamicacid, the abnormal amino acid formed by vitamin K-dependent carboxylase). Therefore we have started to characterize the protein constituents of hardened atherosclerotic plaques.The calcified areas from human aortae were solubilized in EDTA and the proteins extracted were partly purified by batch-wise adsorption onto QAE and elution with high salt. The crudeplaque-extract did not contain prothrombin, factor X or protein C. This excludes the possibility that Gla-containing coagulation factors are bound non-specifically from blood. Osteocalcin accounted for 20% of the total amount of protein-bound Gla-residues.Another Gla-containing protein waspurified from the crude plaque-extract by employing high performance liquid chromatography (HPLC). Gel filtration yielded a Gla-rich protein with anapparent Mr of 25 kD. In vitro boththe crude plaque-extract and the purified Gla-containing protein strongly inhibited the precipitation of calcium phosphate and calcium carbonate. A similar effect was not found with humanserum albumin nor with a thermallydecarboxylated plaque-extract. If also in vivo the Gla-containing proteinsproduced by vessel wall carboxylase prevent the precipitation of calcium salts remains to be investigated.