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Baishya, Ratul, and Rhituporna Saikia. "Phosphate Solubilizing Bacteria in Certain Agricultural Crop Soils of Delhi." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 4, no. 01 (2018): 70–75. http://dx.doi.org/10.18811/ijpen.v4i01.11613.
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Phosphate solubilizing bacteria (PSB) helps in the solubilization of insoluble phosphates and thus lead to increase in crop yields. A study was conducted to isolate and characterize biochemically PSB from different agricultural crop soils of Delhi such as Garlic, Radish, Chilli, Onion and Cabbage. PSB were isolated in Pikovskaya solid medium and formation of solubilization (halo) zone was measured. 16 PSB were isolated and identified. The selected PSB differed in phosphate solubilizing efficiency, production of organic acids and phosphatases. Citrobacter sp. and Pseudomonas sp. were dominant in all the crop plants. Among all the isolates, Pseudomonas sp. proved to be an efficient phosphate solubilizer.
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Bandita Pati and Sanhita Padhi. "Isolation and characterization of phosphate solubilizing bacteria in saline soil from Costal Region of Odisha." GSC Biological and Pharmaceutical Sciences 16, no. 3 (2021): 109–19. http://dx.doi.org/10.30574/gscbps.2021.16.3.0273.
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The present study was conducted to isolate phosphate solubilizing bacteria (PSB) from rhizospheric saline soils of coastal Odisha, India and evaluated their phosphate solubilizing ability. Total four PSB were isolated based on the halo zone formation (solubilizing index 2.63-3.14) on PVK agar medium and were characterized based on biochemical and molecular characteristics as Bacillus subtilis (B1), B. megaterium (B2), Sphingomonas paucimobilis (P2) and Kocuria kristinae (P6). The inorganic phosphate released by PSB ranged from 18.532 to 38.250µg/ml with decreasing the pH PVK broth up to 3.9. Acid phosphatase activity for PSB were recorded 84.237-98.658µmol/min. Glucose was found to be the best carbon source for B. subtilis, Sphingomonas paucimobilis and Kocuria kristinae whereas mannitol for B. megaterium. Optimum acid phosphatase activity was observed for all the four PSB isolates in presence of ammonium sulphate as nitrogen source in PVK broth at 30oC and pH 7.0.
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Fuskhah, E., and E. D. Purbajanti. "Exploration and selection of salt tolerant phosphate solubilizing bacteria from the Indonesian saline soils." IOP Conference Series: Earth and Environmental Science 1246, no. 1 (2023): 012026. http://dx.doi.org/10.1088/1755-1315/1246/1/012026.
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Abstract The excessive salt concentration is what prevents saline land from being used. Salinity inhibits plant growth, however this can be remedied by investigating phosphate-solubilizing bacteria (PSB). Non-pathogenic bacteria that can dissolve unavailable P into a form that plants may absorb are known as phosphate solubilizing bacteria. The goal of this study is to find phosphate-solubilizing bacteria that can withstand extremely high salinities. In saline land on Indonesia’s North Coast of Central Java, soil sampling was done. Using pikovskaya selective media, phosphate solubilizing bacteria were isolated using the pour plate method. Eight pure PSB isolates, including BPFRBG2, BPFPT2, BPFDMK1, BPFPKL2, BPFSMG2, BPFBTG2, and BPFKDL2, were obtained as a result of the isolation process. The macro- and microscopic examination of PSB isolates. Following closely behind were the PSB isolates with the strongest tolerance to salt (10.000 ppm NaCl): BPFBTG2, BPFKDL2, BPFSMG2, and BPFPKL2.
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Bandita, Pati, and Padhi Sanhita. "Isolation and characterization of phosphate solubilizing bacteria in saline soil from Costal Region of Odisha." GSC Biological and Pharmaceutical Sciences 16, no. 3 (2021): 109–19. https://doi.org/10.5281/zenodo.5542913.
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The present study was conducted to isolate phosphate solubilizing bacteria (PSB) from rhizospheric saline soils of coastal Odisha, India and evaluated their phosphate solubilizing ability. Total four PSB were isolated based on the halo zone formation (solubilizing index 2.63-3.14) on PVK agar medium and were characterized based on biochemical and molecular characteristics as <em>Bacillus subtilis</em> (B1), <em>B. megaterium</em> (B2), <em>Sphingomonas paucimobilis</em> (P2) and <em>Kocuria kristinae</em> (P6). The inorganic phosphate released by PSB ranged from 18.532 to 38.250µg/ml with decreasing the pH PVK broth up to 3.9. Acid phosphatase activity for PSB were recorded 84.237-98.658µmol/min. Glucose was found to be the best carbon source for <em>B. subtilis</em>, <em>Sphingomonas paucimobilis</em> and <em>Kocuria kristinae</em> whereas mannitol for <em>B. megaterium</em>. Optimum acid phosphatase activity was observed for all the four PSB isolates in presence of ammonium sulphate as nitrogen source in PVK broth at 30oC and pH 7.0.
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Hnamte, Remruattluanga, and R. Lalfakzuala. "Phosphate Solubilizing Bacteria in Traditional Jhum Field of Mizoram." Science & Technology Journal 6, no. 1 (2018): 39–44. http://dx.doi.org/10.22232/stj.2018.06.01.05.
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Phosphorus is an essential plant nutrient; however its availability for plant uptake is limited. Phosphate Solubilizing Microbes (PSM) are a group of microbes capable of solubilization of inorganic phosphates thereby making it available for plant use. These microbes (PSM) play a vital role on the phosphorus cycle. Among the PSM, Phosphate Solubilizing Bacteria (PSB) are pre dominant and are crucial for maintaining available phosphorus in soil. However during slash and burning of jhum land, soil microbial community is altered to a great extend. The present study investigates the diversity of PSB in jhum field using 16SrRNA gene amplification of isolated potent bacteria. Seven species of PSB were identified, belonging to Burkholderia, Bacillus and Xanthomonas.
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Amri, Marwa, Mohamed Ridha Rjeibi, Marwa Gatrouni, et al. "Isolation, Identification, and Characterization of Phosphate-Solubilizing Bacteria from Tunisian Soils." Microorganisms 11, no. 3 (2023): 783. http://dx.doi.org/10.3390/microorganisms11030783.
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Soil microorganisms play an important role in maintaining natural ecological balance through active participation in carbon, nitrogen, sulfur, and phosphorous cycles. Phosphate-solubilizing bacteria (PSB) are of high importance in the rhizosphere, enhancing the solubilization of inorganic phosphorus complexes into soluble forms available for plant nutrition. The investigation of this species of bacteria is of major interest in agriculture, as they can be used as biofertilizers for crops. In the present study, 28 isolates of PSB were obtained after the phosphate enrichment of soil samples from five Tunisian regions. Five PSB species were identified by 16S rRNA gene sequencing including Pseudomonas fluorescens, P. putida, and P. taiwanensis, Stenotrophomonas maltophilia, and Pantoea agglomerans. Solid and liquid Pikovskaya’s (PVK) and National Botanical Research Institute’s (NBRIP) media containing insoluble tricalcium phosphate were used for the evaluation of the phosphate solubilization ability of the bacterial isolates by two methods: visual evaluation of the solubilization zone around colonies (halo) and determination of solubilized phosphates in liquid medium by the colorimetric method of the vanado-molybdate yellow. Based on the results of the halo method, the isolate of each species that showed the higher phosphate solubilization index was selected for evaluation of phosphate solubilization by the colorimetric method. In the liquid media, the bacterial isolates showed phosphate solubilization ranging from 535.70 to 618.57 µg mL−1 in the NBRIP medium, and 374.20 to 544.28 µg mL−1 in the PVK medium, with the highest values produced by P. fluorescens. The best phosphate solubilization ability and higher reduction in broth pH, which indicates higher organic acid production, were achieved in NBRIP broth for most of the PSB. Strong correlations were observed between the average capability of PSB to solubilize phosphates and both the pH and total phosphorous content in the soil. The production of the hormone indole acetic acid (IAA), which can promote plant growth, was observed for all five PSB species. Among them, P. fluorescens obtained from the forest soil of northern Tunisia showed the highest production of IAA (50.4 ± 0.9 µg mL−1).
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Asril, Muhammad, Erma Suryanti, and Ika Agus Rini. "In Vitro Evaluation of Phosphate Solubilizing Bacteria as Antagonists for Bacterial Leaf Blight Disease (Xanthomonas oryzae pv. oryzae)." Jurnal Riset Biologi dan Aplikasinya 6, no. 1 (2024): 34–40. http://dx.doi.org/10.26740/jrba.v6n1.p34-40.
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Rice is an important food crop in Indonesia, where more than 50% of the Indonesian population consumes rice as a staple food. Rice has decreased productivity by 50-80% due to bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo). The rapid spread and damaging effects of pesticide use on the environment are the main obstacles in the treatment of these bacteria. Alternative control is to use biological agents in the form of phosphate solubilizing bacteria (PSB). Phosphate solubilizing bacteria are part of the growth-promoting bacteria that have other abilities, namely biocontrol. The purpose of this study was to develop the ability of acid soil phosphate solubilizing bacteria to inhibit growth in vitro. The test was carried out using the dual culture technique with the zone of inhibition method. The results showed that ten isolates of PSB had inhibition against Xoo with various diameters of inhibition. The four highest inhibitory isolates were EF.NAP 3 (5.267 ± 0.189 mm), Pseudomonas GSP 6 (4533 ± 0.772 mm), EF.NAP 4 (3,424 ± 0.161 mm) and EF. NAP 9 (3.167±0.136 mm). The ability of PSB to inhibit Xoo can be used as an alternative in controlling bacterial leaf blight in rice plants. This indicates that the PSB isolate acts as a biostimulant in the dissolution of phosphate in the soil and as a bioprotectant.
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ALFIAH, LUFITANUR, DELITA ZUL, and NELVIA NELVIA. "The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean." Nusantara Bioscience 10, no. 3 (2018): 146–50. http://dx.doi.org/10.13057/nusbiosci/n100303.
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Alfiah L, Zul D, Nelvia N. 2018. The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean. Nusantara Bioscience 10: 146-150. Despite the abundant amount of phosphorus (P) in the soil, P uptake by the plants is very limited. In acidic soil, phosphorus (P) is bound to aluminum (Al) and iron (Fe), whereas in the alkaline soil, phosphorus (P) is bound to calcium (Ca). The improvement of efficiency and availability of P to plants can be made by utilizing a group of solubilizing phosphate microorganisms. Potency test to investigate the P solubility by Phosphate Solubilizing Bacteria (PSB) has been conducted by isolating the bacteria from peat soil at Biosphere Reserves of Giam Siak Kecil Bukit Batu, Riau, Indonesia. The semi-quantitative test revealed that the PSB were able to dissolve Ca3 (PO4)2, FePo4 and phosphate rock. However, the adaptation ability and potency of PSB from indigenous Riau peat soil inoculated into soybean (Glycine max L. Merr) plants in the mineral soil have not yet been investigated. The present study was carried out from March to June 2015 on the alluvial soil in Babussalam Village, Rambah Sub-district, Rokan Hulu District, Riau. The aim of this study was to determine the effect of PSB inoculation on bacterial population and phosphatase activity. The study also aimed to determine the available P and P uptake and their impact on soybean growth and production. The study employed a factorial experiment laid out in a completely randomized design (CRD) consisted of two factors, i.e., soil treatment and PSB. The first factor comprised of two levels, i.e., T0: non-sterilized soil, T1: sterilized soil. The second factor consisted of 4 levels, i.e., B0: without PSB inoculation, B1: inoculation using 2 isolates of PSB (BB_UB6 and BB_K9), B2: inoculation using 3 isolates of PSB (BB_UB6, BB_K9 and BB_K2), and B3: inoculation using 4 isolates of PSB (BB_UB6, BB_K9, BB_K2, and BB_HS13). The results showed that inoculation of starter 3 had the highest phosphatase activity rate of 12.43 μg p NP g-1 hour-1. The highest available P was produced by starter 2, while the P uptake on non-sterilized soil was higher than that on the sterilized soil at 2.63 mg plant-1. PSB inoculation and soil sterilization did not significantly affect the population of phosphate solubilizing bacteria.
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Susilowati, L. E., and S. Syekhfani. "Characterization of phosphate solubilizing bacteria isolated from Pb contaminated soils and their potential for dissolving tricalcium phosphate." Journal of Degraded and Mining Lands Management 1, no. 2 (2014): 57–62. http://dx.doi.org/10.15243/jdmlm.2014.012.057.
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Phosphorus solubilizing bacteria (PSB) enhances P availability in soils through dissolving inorganic P pools. The characteristics of PSB and the potential of three Pb tolerant phosphate solubilizing rhizobacteria, i.e. Pseudomonassp, Bacillus sp., and Actinomycites sp. were evaluated. PSB were isolated from soil samples contaminated with 300 and 500 mg Pb/kg after incubation for 30 days. Phosphate solubilizing bacteria were screened on for phosphate solubilisation ability in Pikovskaya agar medium (PA). In addition, two of the three indentified PSB strains (Pseudomonas sp. and Bacillus sp.) were characterized for their ability to solubilize tricalcium phosphate in Pikoskaya broth (PB) and also were examined their growth during culture medium incubation. The isolates exhibited different phosphate solubilization index, ranging from 1.87 to 2.98. Pseudomonas sp. had the highest ability to solubilize tricalcium phosphate: 9.82 mg P/ L and 12.23 mg P/ Lin Pikoskaya broth following the addition of 4 mg Pb/L and 2 mg /Pb L, respectively.Â
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Yanti Ningtyas, Dewi Nurma, Betty Natalie Fitriatin, Mieke Rochimi Setiawati, and Tualar Simarmata. "Pengaruh Pemupukan Bakteri Pelarut Fosfat Dan Dosis Batuan Fosfat Terhadap Ketersediaan Fosfor Pada Tanah Budidaya Tanaman Jagung (Zea Mays L.) Di Ultisol Kentrong." Jurnal Ekonomi Pertanian dan Agribisnis 2, no. 1 (2024): 35–40. https://doi.org/10.62379/jepag.v2i1.1941.
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The use of Ultisols for plant cultivation has constraints, namely the nature of the soil is acidic and the availability of phosphate for plants is low. The use of Rocks Phosphate (RP) and Phosphate Solubilizing Bacteria (PSB) can be used as an alternative to increasing the availability of phosphate for environmentally friendly plants. PSB has the potential to increase the solubility and availability of phosphate in plants. The increase in the availability of phosphate for plants can be through the enzyme phosphatase and organic acids produced by PSB. This study aims to examine the effect of the type of PSB consortium and the dose of Rocks Phosphate on P uptake and growth of maize in Ultisols from Kentrong. The research was conducted at the Ciparanje Experimental Garden, Faculty of Agriculture, Padjadjaran University, Jatinangor. The design used was a randomized block design with factorial pattern consisting of phosphate solubilizing bacteria (without the PSB consortium, exogenous PSB consortium, indigenous PSB consortium, and mixed consortium) and Rocks Phosphate dosage (100% SP-36, 100% RP, 80% RP, 60% RP) with three replications. The results showed no interaction between the type of PSB consortium and the dose of Rocks Phosphate. The results of the independent effect showed that the treatment of using SP-36 was the best treatment in available P.
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Kirui, Charles Kibet, Ezekiel Mugendi Njeru, and Steven Runo. "Diversity and Phosphate Solubilization Efficiency of Phosphate Solubilizing Bacteria Isolated from Semi-Arid Agroecosystems of Eastern Kenya." Microbiology Insights 15 (January 2022): 117863612210889. http://dx.doi.org/10.1177/11786361221088991.
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Phosphorus (P) is a major nutrient required for plant growth but it forms complexes with other elements in soil upon application. A cost-effective way of availing P to plants is by use of Phosphate Solubilizing Bacteria (PSB). There is a wide range of PSB suited for diverse agro-ecologies. A large part of Eastern Kenya especially the lower altitude regions are semi-arid with nutrient depleted soils and predominated by low-income smallholders farmers who do not afford costly inorganic fertilizers. To alleviate poor soil nutrition in this agroecosystem, we sought to study the diversity of phosphate solubilizing bacteria and their phosphate solubilization efficiency. The bacteria were selectively isolated in Pikovskaya’s agar media. Bacterial colonies were enumerated as Colony Forming Units and morphological characterization determined by analyzing morphological characteristics. Genetic characterization was determined based on sequencing of 16S rRNA. A total of 71 PSB were isolated and they were placed into 23 morphological groups. Correlation analysis showed a negative correlation between phosphate solubilizing bacteria and the levels of phosphorus, iron, calcium, magnesium and soil pH. Analysis of 16S rRNA sequences revealed that the genetic sequences of the isolates matched the strains from the genera Burkholderia, Pseudomonas, Bacillus, Enterobacter, Pantoea, Paraburkholderia, Cronobacter, Ralstonia, Curtobacterium, and Massilia deposited in NCBI Database. Analysis of Molecular Variance showed that variation within populations was higher than that of among populations. Phosphate solubilization index values ranged between 1.143 and 5.883. Findings on biodiversity of phosphate solubilizing bacteria led to identification of 10 candidate isolates for plant growth improvement and subsequently, bio-fertilizer development.
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Noor, Aidi. "Improvement of Soil Chemistry Characteristic of Upland with Rock Phosphate, Phosphate-Solubilizing Bacteria and Farm Yard Manure For Increase of Soybean Yield." Journal of Tropical Soils 13, no. 1 (2008): 49–58. http://dx.doi.org/10.5400/jts.2008.v13i1.49-58.
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The experiment aims to know the role of rock phosphate, phosphate-solubilizing bacteria and farm yard manure application in improve quality of acid upland and its influence to soybean yield. Experiment was conducted in the greenhouse using Ultisols from Kentrong at 2001 year. Factorial experiment design with two factors was used in randomized complete block design with three replications. The first factors was rock phosphate dosages i.e. : 0; 30; 60; 90 kg P ha-1, and the second factor was combination of phosphate solubilizing bacteria (PSB) and farm yard manure (FYM) i.e. : without PSB and FYM (control); PSB (Pseudomonas fluorescens); FYM 10 t ha-1, and PSB+FYM. Results indicated that rock phosphate and combination of PSB and FYM application increased available P and decrase exchangeable Al of soils, increase nutrient uptake N, P, nodulation and grain yield of soybean. Application of phosphate solubilizing bacteria and farm yard manure combination with rock phosphate 30 kg P ha-1 dosages average increase grain yield soybean 23.5% compared with control. Grain yield of soybean 87% affected by variables of available P soils (X1), nutrient uptake N (X4) and P (X5), with multiple regression equation : Y = 0.241 + 0.03900 X1 + 0.00470 X4 + 0.04263 X5.
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Aliyat, Fatima Zahra, Mohamed Maldani, Mohammed El Guilli, Laila Nassiri, and Jamal Ibijbijen. "Phosphate-Solubilizing Bacteria Isolated from Phosphate Solid Sludge and Their Ability to Solubilize Three Inorganic Phosphate Forms: Calcium, Iron, and Aluminum Phosphates." Microorganisms 10, no. 5 (2022): 980. http://dx.doi.org/10.3390/microorganisms10050980.
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Biofertilizers are a key component of organic agriculture. Bacterial biofertilizers enhance plant growth through a variety of mechanisms, including soil compound mobilization and phosphate solubilizing bacteria (PSB), which convert insoluble phosphorus to plant-available forms. This specificity of PSB allows them to be used as biofertilizers in order to increase P availability, which is an immobile element in the soil. The objective of our study is to assess the capacity of PSB strains isolated from phosphate solid sludge to solubilize three forms of inorganic phosphates: tricalcium phosphate (Ca3(PO4)2), aluminum phosphate (AlPO4), and iron phosphate (FePO4), in order to select efficient solubilization strains and use them as biofertilizers in any type of soil, either acidic or calcareous soil. Nine strains were selected and they were evaluated for their ability to dissolve phosphate in the National Botanical Research Institute’s Phosphate (NBRIP) medium with each form of phosphate (Ca3(PO4)2, AlPO4, and FePO4) as the sole source of phosphorus. The phosphate solubilizing activity was assessed by the vanadate-molybdate method. All the strains tested showed significantly (p ≤ 0.05) the ability to solubilize the three different forms of phosphates, with a variation between strains, and all strains solubilized Ca3(PO4)2 more than FePO4 and AlPO4.
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Fontaine, Laurent, Nelson Thiffault, David Paré, J. André Fortin, and Yves Piché. "Phosphate-solubilizing bacteria isolated from ectomycorrhizal mycelium ofPicea glaucaare highly efficient at fluorapatite weathering." Botany 94, no. 12 (2016): 1183–93. http://dx.doi.org/10.1139/cjb-2016-0089.
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Fluorapatite-solubilizing bacteria were isolated from the hyphosphere of the ectomycorrhizal (ECM) fungus Wilcoxina sp., a dominant species in the Picea glauca (Moench) Voss rhizosphere. Hundreds of strains isolated from the ascomycete Wilcoxina sp. could dissolve tricalcium phosphate, while only 27 of them could produce clarification halos on fluorapatite-amended solid medium. Most of the fluorapatite-solubilizing strains belonged to the Burkholderia genus. Scanning electron microscopy observations have shown that these efficient phosphate-solubilizing bacteria (PSB) were able to completely solubilize fluorapatite crystals within 22 h. The efficient PSB Burkholderia sp. strain 205 and Curtobacterium sp. strain 168 were tested for their ability to associate with a genetically distant fungal host while fulfilling their phosphate-solubilizing function. Burkholderia sp. strain 205 successfully associated with the basidiomycete Laccaria bicolor (Maire) P.D.Orton when hydroxyapatite was the only phosphorus source available to the fungus, while there was no bacterial development when Laccaria bicolor could access soluble phosphorus as well. Optical microscopic observation of Laccaria bicolor associated with Burkholderia sp. revealed extensive colonization of fungal hyphae by the bacterium. These results suggest an important role of bacteria – ECM fungi associations in white spruce phosphate nutrition.
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Hazra, Fahrizal, and Etty Pratiwi. "Isolation, Characterization, and Molecular Identification of Phosphate Solubilizing Bacteria from Several Tropical Soils." JOURNAL OF TROPICAL SOILS 18, no. 1 (2013): 67. http://dx.doi.org/10.5400/jts.2013.v18i1.67-74.
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The objectives of the research were: (i) to isolate and characterize of phosphate solubilizing bacteria (PSB) and (ii) to identify PSB based on molecular amplification of 16S rRNA gene. Soil samples were collected from rhizosphere in Bogor, West Nusa Tenggara, and East Nusa Tenggara. Several stages in this research were: (i) isolation PSB in Pikovskaya agar, (ii) morphological and biochemical characterization of PSB, (iii) measurement of phosphatase enzymes, and (iv) measurement of secreting indole acetic acid phytohormone. As many as 29 isolates of PSB have been collected and three isolates of them, namely: P 3.5 (East Nusa Tenggara), P 6.2 (West Nusa Tenggara), and P 10.1 (Citeureup, West Java) were chosen for further study. There were many characteristics of isolate P 10.1: (i) it had capable to solubilize P with the value of highest solubilization index (1.80), (ii) it had the highest phosphatase enzyme (120.40 mg kg-1), and (iii) it had the highest pH decrease at each observation for six days. Isolates P 3.5 and P 10.1 were the Gram-negative bacteria with coccus shapes and isolate P 6.2 was a Gram-negative bacteria with bacillus shape. Deoxiribonucleat Acid (DNA) amplification of these bacteria employing 16S rRNA primers generated the 1,300bp-PCR product. The results of the analysis of 16S rRNA gene sequences showed that isolates P 3.5 and P 10.1 has 98% similarity with Gluconacetobacter sp. strains Rg1-MS-CO and isolate P 6.2 has 97% similarity with Enterobacter sp. pp9c strains.Keywords: 16S rRNA, indole acetic acid, isolation, phosphatase enzymes, phosphate solubilizing bacteria[How to Cite : Hazra F and E Pratiwi. 2013. Isolation, Characterization, and Molecular Identification of Phosphate Solubilizing Bacteria from Several Tropical Soils. J Trop Soils, 18 (1): 67-74. doi: 10.5400/jts.2013.18.1.67][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.1.67]
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Suliasih and S. Widawati. "Inorganic and organic phosphate solubilization potential of Stenotrophomonas maltophilia." IOP Conference Series: Earth and Environmental Science 948, no. 1 (2021): 012054. http://dx.doi.org/10.1088/1755-1315/948/1/012054.
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Abstract Most soils contain Phosphorus (P) in insoluble compounds as organic and inorganic forms which is unavailable to plants. Furthermore, phosphate solubilizing bacteria (PSB) play an important role in converting insoluble P to a bioavailable form through solubilizing and mineralizing inorganic or organic P. Therefore, this study aims to determine the ability of isolate a phosphate solubilizing bacterium (PSB), Stenotrophomonas maltophilia to solubilize inorganic and organic P. The bacterium was isolated from peat soil of West Kalimantan using Pikovskaya medium added with Ca3 (PO4)2 as the P source. The activity of organic P (acid phosphatase and phytase) was measured using p-nitrophenyl phosphate disodium (pNPP 0.115 M) and sodium phytate as the substrate of bacterial broth culture. Also, the optimization for inorganic and organic P solubilization by the bacterium was conducted. The maximum values of inorganic and organic phosphate solubilization was recorded to be 52.26 μg/mL and 44.51 U/mL (acid phosphatase), 0.13 U/mL (phytase) respectively. Optimum conditions found were temperature at 30 °C, pH 6.0 and in the presence of sucrose and beef extract, which serve as carbon and nitrogen sources, respectively. Therefore, it can probably be used as a biological fertilizer for plants.
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Lovitna, Gertruda, Yulia Nuraini, and Nurul Istiqomah. "PENGARUH APLIKASI BAKTERI PELARUT FOSFAT DAN PUPUK ANORGANIK FOSFAT TERHADAP POPULASI BAKTERI PELARUT FOSFAT, P-TERSEDIA, DAN HASIL TANAMAN JAGUNG PADA ALFISOL." Jurnal Tanah dan Sumberdaya Lahan 8, no. 2 (2021): 437–49. http://dx.doi.org/10.21776/ub.jtsl.2021.008.2.15.
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Fulfilment of nutrients for plants can be maximized when fertilization, but the application of inorganic fertilizers can cause damage to the soil if the dosage is excessive. The utilization of phosphate solubilizing bacteria is an effort to increase the efficiency of phosphate fertilization that can extract phosphate from an insoluble form that becomes available. This study aimed to obtain a combination of inorganic fertilization doses (SP-36 and NPK) and the application of biological fertilizers that utilize phosphate solubilizing bacteria (PSB) to increase the population of phosphate solubilizing bacteria and P-available in the soil, as well as corn crop yields. This study used a randomized block design with nine treatments and three replications. The result showed that the application of PSB and inorganic phosphate fertilizer shows a significant effect on the population of PSB, P-available, plant height, stover dry weight, cob weight with husk and cob weight without husk, but didn’t show a significant effect on 100 seeds weight and number of plant leaves. Based on the correlation analysis, the results show a positive correlation between soil chemical properties, P-available, and corn yield.
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Dermiyati, D., N. A. Fitri, and R. Suharjo. "Effect of phosphate solubilizing bacteria and phosphate fertilizer on soil bacterial population and some soil chemical properties." IOP Conference Series: Earth and Environmental Science 1482, no. 1 (2025): 012049. https://doi.org/10.1088/1755-1315/1482/1/012049.
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Abstract The agricultural waste from the agricultural processing industry can generate environmental pollution. On the other hand, the organic waste, especially from the Empty Oil Palm Fruit Bunches (EFB) and Pineapple Rhizomes (PR), can be used as bio fertilizer, such as phosphate solubilizing bacteria (PSB). This study examined the effect of applying PSB and the type of phosphate fertilizer (PF) on the soil bacterial population and some soil chemical properties. The study was designed in a 4×3 factorial in a randomized block design (RBD) with three replications. The first factor was the type of PSB (without PSB, PSB from EFB, PSB from PR, and a mixture of PSB from EFB+PR). The second factor was the type of phosphate fertilizer (without PF, SP-36 PF, and Moroccan Natural Rock Phosphate (NRP) Fertilizer. The research was conducted in two parallel experiments, one on incubated soil without plants and one on soil planted with cucumbers. The results showed that the PSB population in the PSB PR treatment gave the best results in soil conditions for incubated soil without plants. Soil incubation with PSB and PF had a higher soil pH and organic C percentage than the control treatment. The PSB population in the PSB PR treatment was higher than in the PSB EFB treatment and the PSB EFB+PR mixture on cucumber-planted soil. The PSB populations, available P, and soil organic C with PSB gave the best results on cucumber-planted soil. PSB population was significantly correlated with the number of cucumber fruit, and soil available P was significantly correlated with root dry weight. Therefore, agro-industry organic waste, especially FEB and PR, can be used as bio fertilizers to improve soil fertility.
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Meena, Deshraj, Ram Hari Meena, Devendra Jain, et al. "Effect of Different Phosphorus Sources on Soil Physico-Chemical Property Dynamics under Chickpea Crop." International Journal of Plant & Soil Science 35, no. 19 (2023): 240–49. http://dx.doi.org/10.9734/ijpss/2023/v35i193549.
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A field experiment was conducted during Rabi season of 2021-2022 to study the effect of rock phosphate (RP), poultry manure (PM) and phosphate solubilizing bacteria (PSB) on soil properties. The experiment was laid out in three replications with 9 treatments in Randomized Block design (RBD). Results revealed that the combined application of 50% P through RP + 50% P through PM + PSB (T9) significantly decrease the bulk density but, higher soil porosity was observed with application of 100% P through PM (T5) and 100% P through PM + PSB (T8) were at par. Whereas, improved in soil pH and organic carbon were reported with the application of T8. Also, the maximum available nitrogen (294.95 kg ha-1) was acquired under T8. However, the highest available phosphorus, potassium and available Fe, Mn, Cu and Zn content in soil were observed with T9. Similarly, higher PSB population and acid phosphatase activity was recorded with application of T9. Dehydrogenase activity was observed to be higher underT8 at harvest of chickpea crop. It was concluded that application of phosphate, poultry manure and phosphate solubilizing bacteria improve the soil properties.
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C., Gnana Sankar, Ajaz Haja Mohideen R., R. Narayanan K., and G. Murugesan A. "INVITRO SELECTION OF PHOSPHATE SOLUBILIZING BACTERIA AND THEIR ROLE IN PLANT GROWTH PROMOTION." International Journal of Computational Research and Development 2, no. 1 (2017): 24–28. https://doi.org/10.5281/zenodo.266599.
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Phosphate solubilizing bacteria (PSB) are known to be able to solubilize different forms of inorganic phosphates. A total of twenty four phosphate Solublizing bacterial colonies were isolated on the Pikovskaya’s (PKV) agar medium, containing insoluble tri-calcium phosphate (TCP), from different pond water sediment sample in and around Tirunelveli district, Tamil Nadu, India. The colonies showing clear halo zones around the bacterial growth were considered as phosphate solubilizers. Out of 24 bacterial isolates, 6 isolates showing highest phosphate solubilisation index (SI) ranged from 1.6-2.5 were selected for the further study as qualitative as well as quantitative estimation of phosphate. Among these 6 potent isolates, <em>Bacillus </em>sp PSB6 showed the maximum phosphate solubilization index of 2.5 in Pikovskaya’s agar plates. A pot culture experiment was conducted to investigate the effects of isolated PSB on early development of plants. Seeds were treated with bacterial strain, and seedlings were harvested 5weeks after inoculation. Pots treated with strains showed a positive effect on plant growth. A significant increment in plant shoot, Root height (21.49, 08.64 cm) and dry weight of Shoot and Root (2.39, 2.21 cm) were determined in plants treated with <em>Bacillus licheniformis (</em>PSB6) has remarkably increased compared to the uninoculated control. This growth promoting action was confirmed by the molecular analysis of genomic DNA by RFLP technique.
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Beralex, BOUEUNAN Wongbe, BONGOUA-DEVISME Affi Jeanne, YOBOUE Kouadio Emile, and BAHAN Lemonou Franck Michaël. "Utilization of Crop Residues and Solubilizing Bacteria to Enhance the Dissolution of Moroccan Phosphate Rock." Asian Journal of Applied Science and Technology 08, no. 03 (2024): 75–88. http://dx.doi.org/10.38177/ajast.2024.8308.
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Phosphorus is essential for plant growth, but its availability in soils is limited due to low solubility of phosphate compounds. This study evaluated the combined effects of crop residues (CR) and phosphate-solubilizing bacteria (PSB) on the dissolution of Moroccan phosphate rock. Pot experiments were conducted for 60 days using different doses of MPR (450 kg/ha, 300 kg/ha, and 150 kg/ha), rice and maize crop residues, and a PSB consortium from a rice paddy field. Parameters such as pH, bacterial count, and phosphorus content were measured. The pH increase was significantly higher under the T3 treatment (CR + PSB) with pH 8.26 to 8.58, followed by T1 (CR alone) with pH 7.73 to 8.1, T2 (PSB alone) with pH 7.4 to 7.91, and T0 (control) with pH 7.27 to 7.67. The number of PSB was highest under the T3 treatment (1238.5 x 10-6 to 2081.1 x 10-6 bacteria/g dry matter), followed by T1 (127.2 x 10-6 to 249.03 x 10-6 bacteria/g dry matter) and T2 with 1.7 x 10-6 to 44.6 x 10-6 bacteria/g dry matter, regardless of the MPR dose. Phosphorus solubilization was 35% to 62% with PSB alone (T2), 72% to 88.5% with CR alone (T1), and over 83.98% when both CR and PSB were combined (T3). This increase was more pronounced with higher MPR doses (300 to 450 kg/ha). Incorporating PSB and crop residues as biofertilizers is a viable strategy for enhancing phosphate rock dissolution and improving soil fertility in agricultural systems.
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GÜLER, Murat. "Isolation and Identification of Phosphate Solubilizing Bacteria (PSB) from the Rhizosphere of Thymus vulgaris L." ISPEC Journal of Agricultural Sciences 8, no. 4 (2024): 978–91. https://doi.org/10.5281/zenodo.13589137.
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Phosphate-solubilizing bacteria dissolve insoluble form of phosphate in the soil with different mechanisms, converting them into a form that can be used, and replaced by chemical fertilizers providing beneficial use of a natural reserve. Although research on phosphate solubilizing bacteria has increased recently, research on the thyme rhizosphere is still limited. It is necessary to investigate different rhizospheric local bacteria that can solubilize phosphate and replace chemical fertilizers. 42 bacterial isolates were obtained from the rhizosphere of Thyme (<em>Thymus</em> <em>vulgaris</em> L.) in this study. Among these isolates, 13 phosphate-solubilizing bacterial isolates were selected which formed a transparent (halo) region around the colonies on Pikovskaya’s Agar (PKA) plates. Isolates were identified using the MALDI-TOF MS method. The morphological, biochemical and IAA production of these isolates as well as quantitative measurements of phosphate solubilization of the isolates in NBRIP broth medium was evaluated. The highest efficiency was noted from <em>Bacillus</em> <em>pumilus</em> PCB-6 with solubilization value of 326.8 ppm. This was followed by <em>Acinetobacter</em> <em>calcoaceticus</em> PCB-3 with solubilization value of 313.8 ppm and <em>Pantoeae</em> <em>agglomerans</em> PCB-4 with solubilization value of 307.4 ppm, respectively. Among the Phosphate solubilizing bacterial isolates, P solubilization index was defined as between 1.6 and 4.2 on PKA agar medium. Additionally, the highest IAA production, at 18.43 µg ml<sup>-1</sup>, was obtained from <em>Pantoeae</em> <em>agglomerans</em> PCB-4. This was followed by <em>Bacillus</em> <em>pumilus</em> PCB-6 with 17.40 µg ml<sup>-1</sup> and <em>Acinetobacter</em> <em>calcoaceticus</em> PCB-3 with 16.71 µg ml<sup>-1</sup>. This study clearly shows that selected local isolates can be used as effective phosphate-based microbial fertilizers.
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Yadav, Anurag, Ravikumar Jadav, Kavin Soni, and Kusum Yadav. "Multifaceted Proficiencies of Thermotolerant Phosphate Solubilizing Bacteria from the Kutch Desert, Gujarat, India." International Journal of Plant & Soil Science 35, no. 20 (2023): 43–55. http://dx.doi.org/10.9734/ijpss/2023/v35i203784.
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Aims: This study aimed to isolate and characterize thermotolerant phosphate solubilizing bacteria (PSB) for potential agricultural applications. We aimed to identify promising PSB isolates and evaluate their traits.
 Study Design: Conducted a comprehensive screening of PSB isolates and selected three top candidates based on their phosphate-solubilizing index. These isolates underwent various tests, including thermotolerance assessment, biochemical profiling, and enzymatic assays. Soluble phosphorus and pH levels were also monitored over time.
 Methodology: Screened multiple PSB isolates, selecting PT26, PT31, and PT35. Conducted growth measurements under elevated temperatures, assessed biochemical profiles, and tested the antibiotic susceptibility. Also monitored soluble phosphorus and pH of PSB isolates over 96 hours and measured acid and alkaline phosphatase activities. Molecular identification was done through 16S rRNA gene sequencing.
 Results: Among the selected PSB isolates, PT35 showed superior thermotolerance and potassium solubilization capacity, while PT26 exhibited indole-3-acetic acid synthesis ability. Soluble phosphorus levels varied among isolates, affecting pH. Acid and alkaline phosphatase activities differed significantly. Molecular identification confirmed PT26, PT31, and PT35 as Enterobacter cloacae, Klebsiella quasipneumoniae, and Enterobacter bugandensis, respectively.
 Conclusion: This study highlights the potential significance of PT35, PT26, and PT31 in agriculture. Their diverse traits and metabolic activities offer adaptability to varying conditions, and thus can benefit soil health and nutrient cycling. These findings contribute to agricultural microbiology research, potentially enhancing sustainable farming practices and soil fertility.
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Sembiring, Albert, and Natalia Lusianingsih Sumanto. "Identification of Potential Isolate Phosphate Solubilizing Bacteria Used 16S rRNA Gene." Bioscientist : Jurnal Ilmiah Biologi 10, no. 2 (2022): 941. http://dx.doi.org/10.33394/bioscientist.v10i2.6218.
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Plants always interact with microorganisms either negatively or positively during their life cycle. The Phosphate Solubilizing Bacteria (PSB) group is one example that has some beneficial effects on the plant. PSB can transform insoluble soil inorganic phosphates into soluble phosphorous so plants can absorb them. This research aimed to test the isolate's ability to solubilize phosphorous and use the 16S rRNA marker gene to identify it. Testing the ability of bacteria to solubilize phosphorous on Pikovskaya's agar medium used a qualitative method by observing the halo zone in the colony around it. Then the bacterial isolate was identified by analyzing the result of sequencing the 16S rRNA gene. The RZ02 bacterial isolate showed the highest performance in solubilizing phosphorous, with a 9.1 mm solubilization index. The gene of 16S rRNA was carried out by using primers 27F and 1492R with an amplicon size of 1500 base pairs. Sequencing analysis and construction of a phylogeny tree were conducted by the Mega X neighbor-joining method and showed 100% similarity with Pseudomonas aeruginosa OIS 481. The solubilization index indicated that the bacteria had a potential candidate to be one of the inoculant components for biofertilizer.
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Song, Juan, LiJing Min, JunRong Wu, Qingfang He, FengMao Chen, and Yang Wang. "Response of the microbial community to phosphate-solubilizing bacterial inoculants on Ulmus chenmoui Cheng in Eastern China." PLOS ONE 16, no. 2 (2021): e0247309. http://dx.doi.org/10.1371/journal.pone.0247309.
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Phosphate-solubilizing bacteria (PSB) have beneficial effects on plant health and soil composition. To date, studies of PSB in soil have largely been performed under field or greenhouse conditions. However, less is known about the impact of introducing indigenous PSB in the field, including their effects on the local microbial community. In this study, we conducted greenhouse and field experiments to explore the effects of the addition of indigenous PSB on the growth of Chenmou elm (Ulmus chenmoui) and on the diversity and composition of the bacterial community in the soil. We obtained four bacterial isolates with the highest phosphate-solubilizing activity: UC_1 (Pseudomonas sp.), UC_M (Klebsiella sp.), UC_J (Burkholderia sp.), and UC_3 (Chryseobacterium sp.). Sequencing on the Illumina MiSeq platform showed that the inoculated PSB did not become the dominant strains in the U. chenmoui rhizosphere. However, the soil bacterial community structure was altered by the addition of these PSB. The relative abundance of Chloroflexi decreased significantly in response to PSB application in all treatment groups, whereas the populations of several bacteria, including Proteobacteria and Bacteroidetes, increased. Network analysis indicated that Chloroflexi was the most strongly negatively correlated with Proteobacteria, whereas Proteobacteria was strongly positively correlated with Bacteroidetes. Our findings indicate that inoculation with PSB (UC_1, UC_M, UC_J, and UC_3) can improve the growth of U. chenmoui and regulate its rhizosphere microbial community. Therefore, inoculation with these bacterial strains could promote the efficient cultivation and production of high-quality plant materials.
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Mussa, EL-Shaymaa E., Sohad F. S. Al-Sharnouby, Amel I. Ramadan, and Walaa H. Ismael. "Exploring Halotolerant Phosphate-Solubilizing Bacteria Isolated from Mangrove Soil for Agricultural and Ecological Benefits." Asian Soil Research Journal 8, no. 4 (2024): 124–41. https://doi.org/10.9734/asrj/2024/v8i4168.
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Phosphorus is a vital element for all life forms. Phosphate-solubilizing bacteria transform phosphate into a bioavailable form through solubilization and mineralization processes. The aim of this study is evaluating the role of halotolerant, phosphate-solubilizing bacteria (PSB). having different PGPR traits as biofertilizers. Thirteen bacterial strains were isolated from soil samples of Ras Muhammad mangrove forest and screened for production of different PGPR traits. The most potent microbial candidates were identified by rRNA gene sequencing analysis and uploaded in GenBank as Bacillus safensis [OR341132] and Cytobacillus firmus [OR431197] these showed ability to tolerate salt concentration up to 10%, can fix nitrogen by growing on nitrogen free media with excellent growth and characterized qualitatively and quantitative solubilize phosphorous more than 100µg/ml of insoluble phosphate, hydrolyse phytate and produced IAA showing the highest value in the range of 94.54, 220,38 μg/mL respectively. In pot trials performed in a greenhouse the isolated strains Bacillus safensis [OR341132] and Cytobacillus firmus [OR431197] were able to significantly improve levels of assimilated phosphate, compared to non-inoculated plants. Moreover, mixed treatment (Bacillus safensis +Cytobacillus firmus ) increased soil fertility and consequently improve the growth of parley plant . The study has overall concluded that bacteria isolated from mangrove soil, Bacillus safensis [OR341132] and Cytobacillus firmus [OR431197] could be used as the halotolerant plant growth-promoting rhizobacteria [HT-PGPR] and phosphate-solubilizing bacteria [PSB] and have a good influence on the health of barley [genotype Giza123] plants under salinity conditions during thirty days.
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Haile, Dereje, Bizuayehu Tesfaye, and Fassil Assefa. "Tomato Production under Synergistic Application of Phosphate Solubilizing Bacteria and Phosphate Amendments." Advances in Agriculture 2023 (March 1, 2023): 1–14. http://dx.doi.org/10.1155/2023/4717693.
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Phosphate solubilizing bacteria have multi-dimensional benefits in broad host range interaction, accessing nutrients, phytohormone induction, stress alleviation, biocontrol activity, and eco-friend approach. This study aimed to evaluate the efficacy of PSB isolates coinoculated with compost, bone meal, and DAP fertilizer on tomato growth response. Tomato seeds were treated with 10 selected PSB isolates separately and grown for 20 days on seedbed, then transplanted to field that was treated with external P-sources and enriched by PSB inoculum. PSB isolates showed positive interaction and achieved significant plant assays including plant height, leaves, branches, flowers, and fruit development. Isolate K-10-41 significantly promoted tomato plant height, floral development, and fruit yield, Mk-20-7 enhanced height and fruit weight whereas K-10-27 induced tomato fruit numbers. Compost application promoted tomato-PSB interaction and induced tomato vegetative growth whereas bone meal was least promotor for most of tomato plant assays. Bone meal was however, one of the top fruit development inducers (harvested 20.94 fruits/plant weighing 881.97 gm). Mixing 50% of recommended compost and DAP fertilizer application enhanced tomato vegetative growth and fruit yield (21 fruits/plant harvested that weighed 872.46 gm). Based on the overwhelming performance, K-10-41 and Mk-20-7 application together with compost and fertilizer mixture were found effective. Therefore, the results of this study imply that application of competent PSB isolates together with nutrient supplements improved symbiotic effectiveness, sustainable production, and environmental health. Consequently, these promising isolates would be recommended for tomato production of higher yield and sustainability after verifying their efficacy at different agroecology and taxonomic identification. Screening potential strains and evaluating their competence under different conditions would be the future perspectives to develop efficient inoculants. Moreover, synergetic application of organic supplements (compost, farmyard, bone meal, or other biowastes), bioinoculants, and proper agrochemicals maximize production and environmental health and is feasible for the economic, social, and ecological sense of balance.
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Javadi Nobandegani, Mohammad Bagher, Halimi Mohd Saud, and Wong Mui Yun. "Phylogenetic Relationship of Phosphate Solubilizing Bacteria according to 16S rRNA Genes." BioMed Research International 2015 (2015): 1–5. http://dx.doi.org/10.1155/2015/201379.
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Phosphate solubilizing bacteria (PSB) can convert insoluble form of phosphorous to an available form. Applications of PSB as inoculants increase the phosphorus uptake by plant in the field. In this study, isolation and precise identification of PSB were carried out in Malaysian (Serdang) oil palm field (University Putra Malaysia). Identification and phylogenetic analysis of 8 better isolates were carried out by 16S rRNA gene sequencing in which as a result five isolates belong to the Beta subdivision ofProteobacteria, one isolate was related to the Gama subdivision ofProteobacteria, and two isolates were related to theFirmicutes. Bacterial isolates of 6upmr, 2upmr, 19upmnr, 10upmr, and 24upmr were identified asAlcaligenes faecalis. Also, bacterial isolates of 20upmnr and 17upmnr were identified asBacillus cereusandVagococcus carniphilus, respectively, and bacterial isolates of 31upmr were identified asSerratia plymuthica. Molecular identification and characterization of oil palm strains as the specific phosphate solubilizer can reduce the time and cost of producing effective inoculate (biofertilizer) in an oil palm field.
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Han, H. S., Supanjani, and K. D. Lee. "Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptáme and growth of pepper and cucumber." Plant, Soil and Environment 52, No. 3 (2011): 130–37. http://dx.doi.org/10.17221/3356-pse.
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Biofertilizers have been used as sources to improve plant nutrients in sustainable agriculture. Experiments were conducted to evaluate the potential of phosphate solubilizing bacteria (PSB) Bacillus megaterium var. phosphaticum and potassium solubilizing bacteria (KSB) Bacillus mucilaginosus inoculated in nutrient limited soil planted with pepper and cucumber. Results showed that rock P and K applied either singly or in combination did not significantly enhance soil availability of P and K, indicating their unsuitability for direct application. PSB was a more potent P-solubilizer than KSB, and co-inoculation of PSB and KSB resulted in consistently higher P and K availability than in the control without bacterial inoculum and without rock material fertilizer. Integrated rock P with inoculation of PSB increased the availability of P and K in soil, the uptake of N, P and K by shoot and root, and the growth of pepper and cucumber. Similar but less pronounced results were obtained when rock K and KSB were added concomitantly. Combined together, rock materials and both bacterial strains consistently increased further mineral availability, uptake and plant growth of pepper and cucumber, suggesting its potential use as fertilizer.
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Panhwar, Qurban Ali, Shamshuddin Jusop, Umme Aminun Naher, Radziah Othman, and Mohd Ismail Razi. "Application of Potential Phosphate-Solubilizing Bacteria and Organic Acids on Phosphate Solubilization from Phosphate Rock in Aerobic Rice." Scientific World Journal 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/272409.
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A study was conducted at Universiti Putra Malaysia to determine the effect of phosphate-solubilizing bacteria (PSB) and organic acids (oxalic & malic) on phosphate (P) solubilization from phosphate rock (PR) and growth of aerobic rice. Four rates of each organic acid (0, 10, 20, and 30 mM), and PSB strain (Bacillussp.) were applied to aerobic rice. Total bacterial populations, amount of P solubilization, P uptake, soil pH, and root morphology were determined. The results of the study showed significantly high P solubilization in PSB with organic acid treatments. Among the two organic acids, oxalic acid was found more effective compared to malic acid. Application of oxalic acid at 20 mM along with PSB16 significantly increased soluble soil P (28.39 mg kg−1), plant P uptake (0.78 P pot−1), and plant biomass (33.26 mg). Addition of organic acids with PSB and PR had no influence on soil pH during the planting period. A higher bacterial population was found in rhizosphere (8.78 log10 cfu g−1) compared to the nonrhizosphere and endosphere regions. The application of organic acids along with PSB enhanced soluble P in the soil solution, improved root growth, and increased plant biomass of aerobic rice seedlings without affecting soil pH.
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Purnomo, Budi, Novi Rahmawati Sutopo, and Yulia Nuraini. "Utilization of indigenous phosphate-solubilizing bacteria to optimize the use of coal fly ash for increasing available-P in an Ultisol." Journal of Degraded and Mining Lands Management 8, no. 4 (2021): 2937–46. http://dx.doi.org/10.15243/jdmlm.2021.084.2937.
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Coal fly ash (CFA) is a coal-burning by-product containing macro and micronutrients, and it is the potential material for improving available P in Ultisols. Phosphate-solubilizing bacteria (PSB) play a role in phosphorus solubilization. This study aimed at elucidating the potential use of phosphate-solubilizing bacteria to optimize the use of coal fly ash for increasing soil available P. This study was conducted in two stages, namely isolation of indigenous PSB from an Ultisol and application of the PSB and CFA to improve soil available P. Five indigenous PSB isolated from the soil had the ability to dissolve phosphate. Isolate B5 could dissolve 9.89 ppm P and had a 99.57% closeness to Pseudomonas stutzeri. The application of 20 and 40 t CFA ha-1 increased the soil pH by 4.2% and 7.2%, respectively. Increasing the dose of CFA decreased the content of available P by 50.6%. However, the combination of PSB and 20 t CFA ha-1 increased soil available P, plant growth, plant dry biomass, and P-uptake by plant.
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Situmorang, Elizabeth Caroline, Andriessa Prameswara, Hana Christine Sinthya, Nurita Toruan-Mathius, and Tony Liwang. "Indigenous Phosphate Solubilizing Bacteria from Peat Soil for an Eco-friendly Biofertilizer in Oil Palm Plantation." KnE Energy 1, no. 1 (2015): 65. http://dx.doi.org/10.18502/ken.v1i1.324.
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<p>Degradation in soil fertility is an enormous problem in agricultural intensification system. Availability in phosphate is one of among encountered problems. Peat soils have large areas and great potential for agriculture and plantations, but have many obstacles in their management. One of the issues is the low soil fertility and low availability of nutrients, especially phosphate which exists in many organic forms that are not available to plants. Phosphorous is an essential element after nitrogen, which plays an important role in plant growth and metabolism and the process of soil microbiology. Introduction of phosphate- solubilizing bacteria (PSB) as biofertilizer, is a renewable energy based which can increase the availability of phosphate to plants can overcome this problem and at the same time reducing the use of inorganic P fertilizer. Indigenous PSB, isolated from palm oil plantation could be used as a better and friendly bio-agent to improve soil fertility, specifically for phosphate availability. Eight PSB have been isolated from peat soil in oil palm platation. Morphological, biochemical characterization and molecular identifications showed that the 8 isolates belonged to Flavobacterium and Enterobacter genera, more precisely the species of Burkholderia sp and Novosphingobium nitrogenifigens. Characterization of isolates of the highest PSB activity consisted of the ability to dissolve phosphate in Pikovskaya solid medium, temperature resistance optimization and the ability to produce phosphatase enzyme. Results showed that isolate with the highest potentiality of dissolving phosphate formed 3.5 cm of clear zone diameter and the phosphatase activity at 9 days of incubation 5.992 units (μmol / ml / min). The peak of enzyme’s activity was reached at 6 days , which corresponded to 54.782 ppm of phosphorus solubilised. The lowest pH (3.2) was reached at 1st day of incubation. These isolates showed the ability to grow optimally at temperature range of 25 to 300C. Indigenous PSB could be used as biofertilizer agents which are eco friendly.</p><p><strong>Keywords</strong>: indigenous; biofertilizer; renewable energy, phosphate solubilizing bacteria;phosphatase; oil palm</p>
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Kautsar, Eva Sofia El, Kusuma Handayani, Salman Alfarisi, and Sumardi Sumardi. "Isolation and Characterization of Cellulolytic Bacteria and Phosphate Solubilizing Bacteria from the Rhizosphere of Muli Banana Plants (Musa acuminata) in Labuhan Ratu District, Bandar Lampung." South Asian Research Journal of Biology and Applied Biosciences 7, no. 03 (2025): 242–48. https://doi.org/10.36346/sarjbab.2025.v07i03.012.
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Muli banana (Musa acuminata) from Lampung requires attention to the availability of phosphate in the soil, as phosphate is one of the most important elements for banana plant growth. To provide dissolved phosphate in the soil, phosphate-solubilizing bacteria (PSB) are needed. Cellulolytic bacteria can provide glucose as a carbon source for PSB. This research aims to isolate and characterize PSB and cellulolytic bacteria from the rhizosphere of Muli banana in Labuhan Ratu district, Bandar Lampung. This research was carried out using soil sampling, bacterial isolation, the selection of potential bacteria, and morphological, physiological, and biochemical characterization. The results showed that out of 5 isolated PSB colonies, BPF4 and BPF8 had the highest phosphate solubility index, reaching a value of 3. The characteristics of the BPF4 and BPF8 are different from each other. Among the 3 colonies with cellulolytic ability, the isolates with the highest cellulolytic activity index were isolates BS1 and BS7, which had an SAI value of 0.58. The morphological results of each cellulolytic bacterial colony varied. The cellulolytic bacteria showed coccus-shaped cells and were Gram-positive in BS1, whereas BS7 was Gram-negative. There was no spreading growth in the PSB motility test, whereas all cellulolytic bacteria exhibited motility. The glucose fermentation test only showed color change in isolates BPF2, BPF5, and BPF8, with no appearance of bubbles. Almost all isolates tested positive in the catalase test, with only isolate BS8 not showing any foam.
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Fitriatin, Betty Natalie, Dita Fauziah, Fabira Nur Fitriani, et al. "Biochemical activity and bioassay on maize seedling of selected indigenous phosphate-solubilizing bacteria isolated from the acid soil ecosystem." Open Agriculture 5, no. 1 (2020): 300–304. http://dx.doi.org/10.1515/opag-2020-0036.
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AbstractPhosphorus availability is the major constraint for plant growth in the acid soil ecosystem, due to high fixation by Al and Fe. Microbial fertilizers such as phosphate-solubilizing bacteria (PSB) can increase P availability in soils for root uptake. The objective of the research was to verify the ability of four isolates of PSB isolated from acid soil to solubilize unavailable inorganic phosphate, produce phosphatase, malic acid and indole acetic acid (IAA), as well as increase plant height of maize seedling. The bioassay by growing maize seedling in liquid nutrients has been performed to study the response of seedling to PSB inoculation. The experimental design of bioassay was a randomized block design with five replications. The results showed that the isolates RR 1 and SPR 4 had a relatively high solubilizing index. Moreover, all the PSB isolates had the ability to produce phosphatase and IAA and dissolve P. The performance of PSB-inoculated seedling was better visually and the root length was increased by 66.7–74.5% compared to the control. This result concludes that the species of four isolates needs to be identified by a biomolecular method and formulated as biofertilizers for increasing the maize productivity in the acid soil ecosystem.
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Panjaitan, Fany Juliarti. "ISOLATION AND CHARACTERISTICS OF PHOSPHATE SOLUBILZING BACTERIA (PSB) FROM VEGETATIVE AND GENERATIVE PHASE OF MAIZE RHIZOSPHERE." JURNAL AGROPLASMA 7, no. 2 (2020): 53–60. http://dx.doi.org/10.36987/agroplasma.v7i2.1834.
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The plants acquire phosporus from soil solution as phosphate anion. The availability of nutrients is very low in soil and crops compared to the other macronutriens. It precipitates in soil as orthophosphate or absorbed by Al and Fe so that inhibiting the plant growth. Phosphate solubilizing bacteria are able to release the P bond of clay minerals and provide it for crops. The research aimed to get phosphate solubilzing microbes from maize (Zea mays L.) rhizosphere. The soil samples were taken from the maize rhizosphere in both the vegetative and generative phases in the Cikabayan Bogor experimental farm. The phosphate solubilzing bacteria were determined for its ability to dissolve phosphate in liquid Pikovskaya media. The results of research were obtained 16 phosphate solubilizing bacteria, each of the 12 isolates derived from maize rhizosphere in vegetative phase (JM FIO) and 4 isolates in generative phase (JT FIO). The phosphate solubiliton index of each phosphate solubilizing bacteria was varied, namely 2,2-4, the largest dissolution index obtained at JM FIO 1. The largest phosphate dissolving ability in liquid Pikovskaya media was showed by JM FIO 3 isolate, P value was 0,60 ppm or increased 300% of control then followed by JM FIO 9 with 0,43 ppm P. The research also showed that JM FIO 3 and JM FIO 9 were not pathogenic and potentially could be used as biological fertilizer with number of cells at each 4.2 x 109 and 1.2 x 109 CFU/g of carrier.Key Words : Phosphate Solubilizing Microbe, Maize, Rhizosphere
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Gandhi, A., G. Muralidharan, and E. Sudhakar. "Isolation and Identification of Elite Phosphate Solubilizing Bacteria from Soil under Paddy Cultivation." International Letters of Natural Sciences 16 (May 2014): 62–68. http://dx.doi.org/10.18052/www.scipress.com/ilns.16.62.
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A considerable number of bacterial species are able to exert a beneficial effect upon plant growth. Mostly they are associated with the plant rhizosphere, so they are called as rhizobacteria. Phosphorus is an essential element for plant development and growth making up about 0.2 % of plant dry weight. Several scientists have reported the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate. Detection and estimation of the phosphate solublization ability of microorganisms have been possible using plate screening methods. Phosphate solubilizers produce clearing zones around the microbial colonies in growth media. In the present investigation a total number of fifteen phosphate solubilizing bacterial colonies isolated from different paddy soils in Cuddalore district of Tamilnadu, India. The isolated PSB were identified and characterized for effective use in the field. All the PSB isolates were identified as Bacillus species and designated as P with serial number from 1 to 15. Among the fifteen isolates, the PSB isolate P6 showed highest amount of phosphate solubilization. The quantity of available phosphorus estimated in the P6 grown Sperber broth culture medium on 7th day was maximum of 321.7 μg/ml which was the highest value compared to other PSB isolates.
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Gandhi, A., G. Muralidharan, and E. Sudhakar. "Isolation and Identification of Elite Phosphate Solubilizing Bacteria from Soil under Paddy Cultivation." International Letters of Natural Sciences 16 (May 30, 2014): 62–68. http://dx.doi.org/10.56431/p-j28hvx.
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A considerable number of bacterial species are able to exert a beneficial effect upon plant growth. Mostly they are associated with the plant rhizosphere, so they are called as rhizobacteria. Phosphorus is an essential element for plant development and growth making up about 0.2 % of plant dry weight. Several scientists have reported the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate. Detection and estimation of the phosphate solublization ability of microorganisms have been possible using plate screening methods. Phosphate solubilizers produce clearing zones around the microbial colonies in growth media. In the present investigation a total number of fifteen phosphate solubilizing bacterial colonies isolated from different paddy soils in Cuddalore district of Tamilnadu, India. The isolated PSB were identified and characterized for effective use in the field. All the PSB isolates were identified as Bacillus species and designated as P with serial number from 1 to 15. Among the fifteen isolates, the PSB isolate P6 showed highest amount of phosphate solubilization. The quantity of available phosphorus estimated in the P6 grown Sperber broth culture medium on 7th day was maximum of 321.7 μg/ml which was the highest value compared to other PSB isolates.
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Joshi, Chinmay, and Manish Soni. "Efficacy of Acinetobacter rudis as Phosphate Solubilizing Bacteria (PSB) on potato growth and nutrient availability." Indian Journal of Science and Technology 16, no. 45 (2023): 4195–99. https://doi.org/10.17485/IJST/v16i45.2755.
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Abstract <strong>Objective:</strong> To determine the effect of Acinetobacter rudis-S23 as a Phosphorus-Solubilizing Bacterium (PSB) on potato growth. <strong>Methods:</strong> A field experiment was conducted in two growing seasons, 2019/2020 and 2020/2021, using a Randomized Complete Block Design (RCBD) with three replications. The treatment group applied A. rudis-S23 as a Phosphate-Solubilizing Bacterium (PSB) to the soil, whereas the control group was untreated. <strong>Findings:</strong> The application of A. rudis-S23 resulted in a significant improvement in various plant growth metrics compared to untreated plants. Specifically, PSB-treated plants exhibited an 18% increase in plant height, a 38% increase in branch number, and a 29% increase in shoot dry weight. The availability of phosphorus in the soil was significantly enhanced by the application of PSB, showing an increase of up to 57% compared to the untreated. Potato tuber absorption of nutrients also saw a substantial improvement, with a 74% increase in tuber nutrient uptake noted in the PSB-treated plants. <strong>Novelty:</strong> This report signifies the pioneering field study of potato plants employing the mangrove endophytic bacterium A. rudis-S23 as a phosphate solubilizer. This bacterium was originally isolated from an unexplored region within the Kutch, Gujarat, India, marking a significant contribution to the scientific understanding of this unique microbial resource. <strong>Keywords:</strong> Acinetobacter rudis, Biofertilizer, Phosphorus Solubilizing Bacteria, Potato
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MATTOS, BIANCA BRAZ, IVANILDO EVÓDIO MARRIEL, SYLVIA MORAIS DE SOUSA, et al. "SORGHUM GENOTYPES RESPONSE TO INOCULATION WITH PHOSPHATE SOLUBILIZING BACTERIA." Revista Brasileira de Milho e Sorgo 19 (December 22, 2020): 14. http://dx.doi.org/10.18512/rbms2020v19e1177.
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Sorghum bicolor adapts to phosphorus (P) deficient soils through mechanisms that contribute to its absorption and solubilization, including the association with microorganisms. The direct application of rock phosphate (RP) and the inoculation with phosphate solubilizing bacteria (PSB) is a sustainable alternative for P supply to the crops. The aim of this study was to evaluate the effect of PSB inoculation of two sorghum genotypes with different P responses (BR007 - efficient and responsive and SC283 - efficient and non-responsive), cultivated in soil fertilized with RP and triple superphosphate (TSP), in greenhouse and field experiments. The sorghum genotypes were inoculated separately with the Bacillus strains that are efficient in P solubilization, B116 and B70, and cultivated under different P fertilization sources (TSP, RP, ½TSP + ½RP). The results suggest that the inoculation response was dependent on sorghum genotype, P source and microbial strain. Inoculation of the genotype BR007 significantly increased root biomass and grain P content under greenhouse conditions, as well as yield and grain P content in field experiments, but no effect was observed on genotype SC283. The use of PSB as bioinoculants, in combination with RP, is a promising alternative to reduce the use of synthetic fertilizers, contributing to the sustainable sorghum production.
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Kartika, Kartika, Abdul Munif, Endah Retno Palupi, Satriyas Ilyas, and Muhamad Rahmad Suhartanto. "Isolation and Characterization of Phosphate Solubilizing Bacteria from Upland Rice Cultivation Areas in Bangka Regency." Journal of Tropical Biodiversity and Biotechnology 9, no. 1 (2024): 84500. http://dx.doi.org/10.22146/jtbb.84500.
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The availability of phosphorus (P) in ultisol acid soils presents a significant challenge due to its attachment to aluminum (Al) or iron (Fe) compounds. A potential solution to address this issue is the utilization of phosphate solubilizing bacteria (PSB). Therefore, this study aimed to analyze the potential of PSB originating from upland rice cultivation on ultisol soils. The bacterial isolates were obtained from soil samples taken from the rhizosphere area and root tissue of upland rice plants cultivated in Payabenua and Saing Villages, Bangka Regency. The pathogenicity testing encompassed hypersensitivity and hemolysis tests, while the P solubilization included the evaluation of the phosphate solubilizing index (PSI) and P dissolution. Subsequently, the selected isolates were subjected to phosphatase enzyme and organic acid content assessment. The results showed a total of 120 isolates, predominantly distributed in the Payabenua area and primarily consisting of endophytic bacteria. Among the six selected isolates, genus Burkholderia dominated four isolates, while the remaining isolates belonged to genus Serratia. Furthermore, in Burkholderia vietnamiensis, the solubility value of P in AlPO4 and Ca3(PO4)2 liquid media exhibited a range of 0.0013 to 0.0344% and 0.0008 to 0.1842%, respectively.
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Kiprotich, Kelvin, John Muoma, Dennis O. Omayio, Tavasi S. Ndombi, and Clabe Wekesa. "Molecular Characterization and Mineralizing Potential of Phosphorus Solubilizing Bacteria Colonizing Common Bean (Phaseolus vulgaris L.) Rhizosphere in Western Kenya." International Journal of Microbiology 2023 (March 1, 2023): 1–10. http://dx.doi.org/10.1155/2023/6668097.
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Phosphorus solubilizing bacteria (PSB) are a category of microbes that transform insoluble phosphates in soil into soluble forms that crops can utilize. Phosphorus in natural soils is abundant but poorly soluble. Hence, introducing PSB is a safer way of improving its solubility. The aim of this study was to genetically characterize and determine the mineralization capability of selected PSB colonizing rhizospheres of common beans in Western Kenya. Seven potential phosphorus solubilizing bacteria (PSB) were isolated from various subregions of Western Kenya. 16S ribosomal RNA gene sequencing and National Center for Biotechnology Information (NCBI), Basic Local Alignment Search Tool (BLAST) identified the isolates. The phosphate solubilization potential of the isolates was evaluated under agar and broth medium of National Botanical Research Institute’s phosphate (NBRIP) supplemented with tricalcium calcium phosphate (TCP). Identified isolates were as follows: KK3 as Enterobacter mori, B5 (KB5) as Pseudomonas kribbensis, KV1 as Enterobacter asburiae, KB3 as Enterobacter mori, KK1 as Enterobacter cloacae, KBU as Enterobacter tabaci, and KB2 as Enterobacter bugandensis. The strains B5 and KV1 were the most effective phosphorus solubilizers with 4.16 and 3.64 indices, respectively. The microbes converted total soluble phosphate concentration in broth medium which was 1395 and 1471 P μg/mL, respectively. The least performing isolate was KBU with a 2.34 solubility index. Significant ( p ≤ 0.05) differences in plant biomass for Rose coco and Mwitemania bean varieties were observed under inoculation with isolates B5 and KV1. PSB isolates found in common bean rhizospheres exhibited molecular variations and isolates B5 and KV1 are the potential in solving the insufficiency of phosphorus for sustainable crop production.
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Luo, Dan, Ju Shi, Mei Li, et al. "Consortium of Phosphorus-Solubilizing Bacteria Promotes Maize Growth and Changes the Microbial Community Composition of Rhizosphere Soil." Agronomy 14, no. 7 (2024): 1535. http://dx.doi.org/10.3390/agronomy14071535.
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Phosphorus deficiency severely limits crop yields and hinders sustainable agricultural development. Phosphate-solubilizing bacteria (PSB) are beneficial for crop growth because they enhance the uptake and utilization of phosphorus. This study explored the phosphorus-solubilizing, IAA-producing, nitrogen-fixing, potassium-solubilizing, and siderophore-producing abilities of three bacterial strains (Pantoea sp. J-1, Burkholderia cepacia Z-7, and Acinetobacter baumannii B-6) screened from the maize rhizosphere. A pot experiment was also conducted to explore the role of screened PSB in the growth of maize. Finally, the effects of the PSB on the physicochemical properties, enzyme activities, and microbial community structure of maize rhizosphere soil were analyzed. The results showed that strain Z-7 had the strongest abilities phosphorus solubilization, nitrogen fixation, potassium solubilization, and siderophore production, while strain J-1 exhibited the highest yield of IAA. The application of PSB promoted the growth of maize plants to different extents. Among the different treatments, the mixed bacterial treatment (J-1 + Z-7 + B-6) had the most potent growth promotion effect, and the consortium treatment significantly enhanced the activity of soil phosphatase. Soil pH, total phosphorus (TP), total potassium (TK), available phosphorus (AP), NH4+-N, and NO3−-N are key factors for the growth of maize plants. In addition, PSB significantly altered the microbial community structure in the maize rhizosphere soil, and the relative abundance of Proteobacteria increased by 16.07–69.10% compared to the control. These PSB have obvious growth-promoting abilities, with the potential to enhance crop productivity as excellent candidate strains for the development of biological fertilizers.
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Lee, Kang-Kook, In-Kyu Mok, Min-Ho Yoon, Hye-Jin Kim, and Doug-Young Chung. "Mechanisms of Phosphate Solubilization by PSB (Phosphate-solubilizing Bacteria) in Soil." Korean Journal of Soil Science and Fertilizer 45, no. 2 (2012): 169–76. http://dx.doi.org/10.7745/kjssf.2012.45.2.169.
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Espinosa-Victoria, David, Lucía López-Reyes, and Aldo De La Cruz-Benítez. "USE OF 16S rRNA GENE FOR CHARACTERIZATION OF PHOSPHATE-SOLUBILIZING BACTERIA ASSOCIATED WITH CORN." Revista Fitotecnia Mexicana 32, no. 1 (2009): 31–37. http://dx.doi.org/10.35196/rfm.2009.1.31-37.
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Thirty-six strains of phosphate-solubilizing bacteria (PSB) isolated from the rhizosphere and rhizoplane of corn (Zea mays L.) crops in different states of México were subjected to phenotypic and genotypic characterization. The phosphate-solubilizing activity of each strain was first evaluated using tricalcium phosphate as the phosphorus source in the NBRIP-BPB culture medium. Phosphatesolubilizing capacity was also evaluated by adding the pH buffering agent MES (2-[Morpholine] ethanosulfonic acid) to the growth medium. Amplified ribosomal RNA restriction pattern analysis (ARDRA) was used to evaluate the genetic diversity. From the data matrix obtained, a dendrogram was built using the UPGMA method. The 16S rRNA gene of the BUAP29, BUAP36 and CP08 strains was amplified, cloned and sequenced for taxonomic identification. The 36 bacterial strains exhibited different levels of tricalcium phosphate solubilizing activity. Only BUAP33, BUAP17 and BUAP21 strains did not show the typical solubilization halo when the MES buffering agent was added to the growth medium. The analysis of ARDRA patterns as well as the dendrogram exhibited a large genetic diversity among the 36 PSB analyzed, with BUAP36 and BUAP15 strains showing 100 % similarity. The 16S rRNA gene sequence alignment of CP08, BUAP29 and BUAP36 strains showed 99 % identity with the sequences of Advenella incenata strain R-16599 (NCBI accession number AY569458.1), Burkholderia sp (NCBI accession number AY353696) and Burkholderia gladioli strain 223-1 (NCBI accession number DQ355168.1), respectively. In this study the A. incenata strain is reported as a PSB for the first time.
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Cahyani, V. R., A. H. N. Fadhilah, A. R. Nabawi, and F. P. B. Krisdhiarto. "Comparing the population density, colony diversity, and phosphate solubilization ability between rice root endophytic and rice rhizospheric soil bacteria from organic wetland." IOP Conference Series: Earth and Environmental Science 1362, no. 1 (2024): 012028. http://dx.doi.org/10.1088/1755-1315/1362/1/012028.
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Abstract Phosphate-solubilizing bacteria (PSB) play important roles in the agricultural systems. The present study aims to compare the population density, colony diversity, and phosphate solubilization ability between rice root endophytic bacteria (RRoEB) and rice rhizospheric soil bacteria (RRzSB) from organic wetland. By spread plate method, bacteria were isolated directly using Pikovskaya’s agar and indirectly through other media, namely Nutrient Agar (NA), Starch Casein Agar (SCA), Jensen, and Yeast Extract Mannitol Agar (YEMA). Each distinct colony based on the morphology on the respective media was screened for phosphate solubilization ability by subculturing on Pikovskaya’s agar. The phosphate solubilizing index (PSI) was measured from the halo zone formation. The results showed that by direct isolation, 6 RRoEB and 5 RRzSB were able to phosphate solubilization. By indirect isolation from NA, SCA, Jensen, and YEMA, 2, 3, 1, and 3 isolates of RRoEB and 4, 3, 1, and 3 isolates of RRzSB showed the capability as phosphate solubilizer. Although the highest colony diversity of PSB was obtained from direct isolation, however the highest PSI was yielded from indirect isolation, namely isolates J1R1 (3.53) and Y1S1 (2.48). The highest PSI from direct isolation was obtained by P1R5 (1.90) and P1S1 (1.51).
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T. S., Koli, Karande R. A., Deshmukh D. P., Waghmare S. J., and Mahajan S. B. "Isolation and Characterization of Azotobacter and Phosphate Solubilizing Bacterial Isolates." Journal of Scientific Research and Reports 30, no. 8 (2024): 755–60. http://dx.doi.org/10.9734/jsrr/2024/v30i82296.
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Isolation from 20 soil samples collected from rhizosphere of watermelon was done on Ashby's medium and Pikovskaya's agar media.8 Azotobacter and 6 Phosphate solubilizing bacteria PSB isolates were obtained from all samples. All the obtained isolates of Azotobacter sp and PSB were gram negative. On morphological study it was observed that, all Azotobacter isolates were motile and positive for KOH test. In case of PSB, the colony shapes of 4 obtained isolates were circular and irregular in 2. All the isolates showed smooth appearance on the media with white colony colour. The biochemical results for both Azotobacter spp and PSB revealed that, most of them were positive for methyl red test, catalase test, starch hydrolysis, gelatine hydrolase test, oxidase test and indol test. Out of eight Azotobacter isolates, Isolate 4 (Azob-1) showed highest (14.25 mg/ml) N fixing ability whereas, from of six PSB isolates, Isolate1 (PSB-1) showed highest solubilising index (4.13) therefore these two isolates were found most efficient in their roles and therefore recommended for use for field experiments.
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Shrivastava, Manoj, Yogendra S. Rajpurohit, Hari S. Misra, and S. F. D’Souza. "Survival of phosphate-solubilizing bacteria against DNA damaging agents." Canadian Journal of Microbiology 56, no. 10 (2010): 822–30. http://dx.doi.org/10.1139/w10-067.
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Phosphate-solubilizing bacteria (PSBs) were isolated from different plant rhizosphere soils of various agroecological regions of India. These isolates showed synthesis of pyrroloquinoline quinone (PQQ), production of gluconic acid, and release of phosphorus from insoluble tricalcium phosphate. The bacterial isolates synthesizing PQQ also showed higher tolerance to ultraviolet C radiation and mitomycin C as compared to Escherichia coli but were less tolerant than Deinococcus radiodurans . Unlike E. coli, PSB isolates showed higher tolerance to DNA damage when grown in the absence of inorganic phosphate. Higher tolerance to ultraviolet C radiation and oxidative stress in these PSBs grown under PQQ synthesis inducible conditions, namely phosphate starvation, might suggest the possible additional role of this redox cofactor in the survival of these isolates under extreme abiotic stress conditions.
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Pathak, Animesh, S. K. Chakrabarti, Rajib Das, and M. K. Mandal. "Response of different wheat varieties towards Azospirillum and phosphate solubilizing bacteria (PSB) seed inoculation." Journal of Applied and Natural Science 8, no. 1 (2016): 213–17. http://dx.doi.org/10.31018/jans.v8i1.775.
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Present investigation was undertaken to utilize Azospirillum and Phosphate Solubilizing Bacteria (PSB) to avail atmospheric nitrogen and soluble phosphate in soil for the wheat crop. The sonalika variety (V1) responded best towards seed inoculation by Azospirillum and Phosphate Solubilizing Bacteria (PSB) for the character speed of germination which is regarded as very important indicator of seed vigour. Germination percentage for untreated control was 86.6% after one month of storage and found to be reduced to 76.67% after three month of storage but for treated Azospirillum in case of sonalika it was retained at 83.33%. V1T1, V2T1 and V3T1 recorded improved germination even after six month of storage. The seedling characters like shoot and root length was greatly improved with T1 and T2 for almost the varieties included in this programme. The character vigour index greatly enhanced in case of V1T1, V1T2, V3T1 and V3T2 after one month and six months of storage. This work reported utilization of Azospirillum and PSB as seed inoculation for wheat varieties to enhance seed storability and seedling growth parameter. It can be concluded that, Azospirillum and PSB seed inoculation can be recommended for wheat for better seedling growth storability of seed.
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R. Saikia and L.R. Saikia. "Effect of pH and salinity on mineral phosphate solubilization by bacterial strains isolated from the rhizosphere of Lablab purpureus (L.) Sweet." Ecology, Environment and Conservation 30, Suppl. (2024): S95—S100. https://doi.org/10.53550/eec.2024.v30i07s.018.
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Phosphate Solubilizing Bacteria (PSB) solubilizes insoluble phosphates. Two strains of PSB, 2b3 and 2b5 were isolated from Lablab purpureus (L.) Sweet rhizosphere soil based on the formation of halozone in Pikovskaya agar plates. Phosphate solubilization in Pikovskaya broth containing either aluminium, ferric or tricalcium phosphates in different pH conditions was estimated. Both the strains were subjected to salinity stress. Morphology and biochemical characters of the bacterial isolates were studied and identified by 16s rRNA gene sequencing. The two bacterial strains 2b3 and 2b5 were identified as Burkholderia sp. and Burkholderia cepacia respectively. The Phosphate Solubilization Index of Burkholderia sp. and Burkholderia cepacia were 2.43±0.057 and 2.36 ± 0.200 respectively. Both the strains showed higher phosphate solubilization in broth containing tricalcium phosphates in comparison to aluminium and ferric phosphates. Burkholderia cepacia (2b5) showed higher phosphate solubilization than 2b3 (Burkholderia sp.) after 144 hours in all the three media. Phosphate solubilized in broth containing aluminium phosphate, ferric phosphate and tricalcium phosphate by 2b5 after 144 hours were 117.53±9.13, 109.15±5.43 and 260.17±11.81 respectively. Both the isolates showed ammonia production and Burkholderia cepacia (2b5) showed better growth in agar plates with NaCl than Burkholderia sp. (2b3). The results indicate that the isolated bacterial strains are potent mineral phosphate solubilizers and further studies will help in the use of these two strains as biofertilizers.
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Khunt, M. D., H. D. Bhimani, Lalit Mahatma, V. A. Solanki, M. M. Patel, and Viral Patel. "Investigation of Halo-tolerant Phosphate Solubilizing Microbes on Wheat (Triticum aestivum L.) under Saline Conditions." Asian Journal of Soil Science and Plant Nutrition 11, no. 2 (2025): 101–10. https://doi.org/10.9734/ajsspn/2025/v11i2514.
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Phosphorus deficiency is the most common problem in the salinity affected areas as the water soluble phosphate rapidly converts into insoluble and plant un-utilizable form. Among different remediation strategies of this problem, use of plant beneficial phosphate solubilizing microbes (PSMs) is cost effect and eco-friendly measure. Two most potent halo-tolerant PSMs i.e. one phosphate solubilizing bacteria (PSB) and one phosphate solubilizing fungi (PSF) were screened on Pikovskaya’s agar amended with 5% NaCl. Bacterial isolate Bacillus subtilis PSB-S showed positive ACC deaminase activity, potash mobilization, IAA production, siderophore activity and Cladosporium herbarum PSF-S found positive for potash mobilization and antagonistic potential against plant pathogenic Fusarium oxysporum on the basis of different in vitro plant growth promoting traits. Field experimental data suggested that application PSB+PSF in combination, along with 100% recommended dose of chemical phosphatic fertilizer (RDP) in wheat under saline stress conditions showed increase in root length (28.30%), shoot length (41.16%), no. of tillers/plant (14.96%), no. of spikes/plant (6.86%), available phosphorus (27.54%) kg/ha, phosphorus uptake (29.03%), seed weight/plant (15.50%), seed (10.52%) and straw yield (6.40%) over full RDP un-inoculated control. Therefore, it is evident from field experimental data that application of these halo-tolerant PSMs under salinity conditions significantly improved phosphorus bioavailabilty and could save 50% of chemical phosphatic chemical fertilizers without compromising yield.