Relevant bibliographies by topics / Phosphates de calcium amorphes

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Academic literature on the topic 'Phosphates de calcium amorphes'

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Published: 4 June 2021
Last updated: 7 February 2022

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Journal articles on the topic "Phosphates de calcium amorphes"

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Dorozhkin, Sergey V. "Amorphous Calcium Phosphates." Journal of Biomimetics, Biomaterials and Tissue Engineering 7 (October 2010): 27–53. http://dx.doi.org/10.4028/www.scientific.net/jbbte.7.27.

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Amorphous calcium phosphates (ACPs) represent a unique class of biomedically relevant calcium orthophosphate salts, in which there are neither translational nor orientational long-range orders of the atomic positions. Nevertheless, the constancy in their chemical composition over a relatively wide range of preparation conditions suggests the presence of a well-defined local structural unit, presumably, with the structure of Ca9(PO4)6 – so-called Posner’s cluster. ACPs have variable chemical but rather identical glass-like physicochemical properties. Furthermore, all ACPs are thermodynamically unstable compounds and, unless stored in dry conditions or doped by stabilizers, spontaneously they tend to transform to crystalline calcium orthophosphates. Although some order within general disorder is the most distinguishing feature of ACPs, the solution instability of ACPs and their easy transformation to crystalline phases might be of a great biological relevance. Namely, the initiating role ACPs play in matrix vesicle biomineralization raises the importance of this phase from a mere laboratory curiosity to that of a key intermediate in skeletal calcification. Furthermore, ACPs are very promising candidates to manufacture artificial bone grafts.
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Vecbiskena, Linda, Luigi de Nardo, and Roberto Chiesa. "Nanostructured Calcium Phosphates for Biomedical Applications." Key Engineering Materials 604 (March 2014): 212–15. http://dx.doi.org/10.4028/www.scientific.net/kem.604.212.

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This work is focused on the phase transformation from amorphous calcium phosphate (ACP) to nanostructured hydroxyapatite (HA) or tricalcium phosphate (TCP). Amorphous calcium phosphates with Ca/P molar ratio near 1.67 and 1.5 were synthesized by wet-chemical precipitation method and treated with ethanol. Upon thermal treatment, ACP clusters about 50 nm create a nanostructured HA or TCP. The highlights of this research: The precipitate treatment with ethanol provided a pure α-TCP that was found to be stable up to 1000 °C. HA is obtained from the ACP precursor synthesized using also ammonium dihydrogen phosphate.
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Dorozhkin, Sergey V. "Amorphous calcium (ortho)phosphates." Acta Biomaterialia 6, no. 12 (December 2010): 4457–75. http://dx.doi.org/10.1016/j.actbio.2010.06.031.

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Holt, Carl, Mathea J. J. M. van Kemenade, Lowell S. Nelson, Lindsay Sawyer, John E. Harries, Raymond T. Bailey, and David W. L. Hukins. "Composition and structure of micellar calcium phosphate." Journal of Dairy Research 56, no. 3 (May 1989): 411–16. http://dx.doi.org/10.1017/s0022029900028880.

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SummaryMicellar calcium phosphate has the chemical composition and physicochemical properties that are consistent with it being a complex of the phosphate centres of casein with an acidic amorphous calcium phosphate. Similar acidic amorphous calcium phosphates have been prepared in the laboratory and for these, as well as for micellar calcium phosphate, the most appropriate crystalline model compound from which the short-range structure may be derived is brushite, CaHPO4.2H2O. The predicted secondary structures around sites of phosphorylation in the Ca2+-sensitive caseins often comprise an α-hclix-loop-α-helix motif with the sites of phosphorylation in the loop region. This motif may be important in linking the colloidal calcium phosphate with casein in native casein micelles.
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Gross, Kārlis. "Towards Biotechnological Processing of Calcium Phosphates." Advanced Materials Research 1117 (July 2015): 193–200. http://dx.doi.org/10.4028/www.scientific.net/amr.1117.193.

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Transient or metastable phases offer an intermediate phase with additional flexibility for creating the end product. The processing pathway remains unknown when the final product is viewed. Biological processes frequently employ the amorphous phase as the transition phase. This is shown in mineralized tissues: invertebrates, pathological calcified deposits and murine fetal teeth. After a fast transition from an amorphous calcium phosphate to crystalline material, smaller changes occur over time. For an appreciation of the transition state, crystallinity is defined and measurement methods outlined. Biotechnology using transition material states offers fast, low temperature access to nanosized high temperature phases. Alfa tricalcium phosphate and apatite is made by a phase transition, but peroxyapatite requires long-range diffusion within the same structure. Tetracalcium phosphate is also possible, but this requires multiple transition states and phase decomposition. The pathway via the amorphous state offers an alternative route to biologically important materials.
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Brangule, Agnese, Līga Avotiņa, Artūrs Zariņš, Mihails Haļitovs, Kārlis Agris Gross, and Gunta Ķizāne. "Thermokinetic Investigation of the Drying Conditions on Amorphous Calcium Phosphate." Key Engineering Materials 758 (November 2017): 204–9. http://dx.doi.org/10.4028/www.scientific.net/kem.758.204.

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The present work investigated dried calcium phosphate powders which still retain an amorphous or poorly crystalline structure under a variety of conditions. In previous studies, freeze-drying was found to be the optimum drying condition. However, several publications, as well as our previous studies, have shown that calcium phosphate amorphous, or a poorly crystalline structure, can retain their structure even if the samples are dried immediately after synthesis up to 200°C. In our study, we used the thermokinetic studies FTIR and XRD and showed that the samples are amorphous, or poorly crystalline, but were unable to answer the questions: Is there a difference between the differently dried amorphous calcium phosphates? What are the optimum drying conditions under which the amorphous calcium phosphate (ACP) structure loses the physically bounded water, but still retains the chemically bounded water?
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Tas, A. Cuneyt. "Calcium metal to synthesize amorphous or cryptocrystalline calcium phosphates." Materials Science and Engineering: C 32, no. 5 (July 2012): 1097–106. http://dx.doi.org/10.1016/j.msec.2012.01.024.

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Lapeyrie, F., J. Ranger, and D. Vairelles. "Phosphate-solubilizing activity of ectomycorrhizal fungi in vitro." Canadian Journal of Botany 69, no. 2 (February 1, 1991): 342–46. http://dx.doi.org/10.1139/b91-046.

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An easy to use method is presented here to compare and study the mineral phosphate-solubilizing activity of ectomycorrhizal fungi. This technique can discriminate between strains with differing phosphate-solubilizing activities. Synthetic mineral phosphates, crystalline or amorphous, were differentially solubilized by 10 ectomycorrhizal fungi. Natural crystalline phosphates studied do not seem to be solubilized by fungi under similar experimental conditions. Paxillus involutus 1 appears to be able to solubilize calcium phosphates using either ammonium or nitrate nitrogen, but the other isolates were able to effectively solubilize phosphate only in the presence of ammonium. This has implications regarding the possible mechanism used to solubilize phosphate by these isolates. Recrystallization can be seen in the culture medium if calcium ions are present. The type of crystals depends on the phosphate source and on the fungal strain. This technique is suitable for screening a large number of ectomycorrhizal strains. The significance of phosphate-solubilizing activity to plant growth stimulation needs to be determined by field trials. Key words: ectomycorrhizal fungus, phosphate, solubilization.
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Xu, J. L., Khiam Aik Khor, R. Kumar, and P. Cheang. "RF Induction Plasma Synthesized Calcium Phosphate Nanoparticles." Key Engineering Materials 309-311 (May 2006): 511–14. http://dx.doi.org/10.4028/www.scientific.net/kem.309-311.511.

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It was a normal phenomena that hydroxyapatite (HA) decomposes into tricalcium phosphate (TCP), tetracalcium phosphate (TTCP), calcium oxide (CaO) and amorphous calcium phosphate (ACP) during the plasma processing step. The present study characterized the phase evolution of calcium phosphates (CaPs) in the nanoparticles synthesized using a radio frequency (RF) induction plasma processing technique. The morphology and microstructure of the CaP nanoparticles were investigated by XRD, SEM, TEM, Raman spectroscopy, FTIR spectroscopy and thermal analysis. It was found that ACP, α-TCP, TTCP and CaO were the main decomposed phases in the nanoparticle. After heat treatment at dicalcium pyrophosphate (β-Ca2P2O7) due to the extreme decomposition of the starting HA during the RF plasma processing step which rapidly solidified into amorphous phase.
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LeGeros, Racquel Z., Dindo Q. Mijares, J. Park, X. F. Chang, I. Khairoun, Regina Kijkowska, Renata Dias, and John P. LeGeros. "Amorphous Calcium Phosphates (ACP): Formation and Stability." Key Engineering Materials 284-286 (April 2005): 7–10. http://dx.doi.org/10.4028/www.scientific.net/kem.284-286.7.

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Our earlier studies showed that several ions inhibit the crystal growth of apatite and promote the formation of amorphous calcium phosphates (ACP). These ions include: magnesium (Mg), zinc (Zn), stannous (Sn), ferrous (Fe), carbonate (CO3), pyrophosphate (P2O7). The purpose of this study was to investigate the effect of combination of these ions (e.g., Mg & CO3, Mg & P2O7, Mg & Zn, etc) on the formation and stability of ACP. ACP compounds containing the different ions were prepared at 25 and 37oC according to the method we previously described. Chemical stability was investigated by suspending the different ACP preparations in solutions with or without inhibitory ions. Thermal stability was determined by sintering the ACP at different temperatures. Dissolution properties were determined in acidic buffer. The ACP before and after chemical or thermal treatment were analyzed using X-ray diffraction, infrared spectroscopy, and thermogravimetry. Results showed synergistic effects of inhibitory ions on the formation of ACP. ACP materials, regardless of their composition, remained amorphous even after heat treatment at 400oC. Transformation of ACP to other calcium phosphate phases depended on the pH and on the solution composition.
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Dissertations / Theses on the topic "Phosphates de calcium amorphes"

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Mayen, Laëtitia. "Etude physico-chimique d'une nouvelle famille de matériaux mixtes ortho- et pyro-phosphates de calcium à visée biomédicale." Thesis, Toulouse 3, 2019. http://www.theses.fr/2019TOU30222.

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Ce mémoire porte sur l'étude de nouveaux matériaux amorphes mixtes orthophosphate et pyrophosphate de calcium et de sodium qui présentent un intérêt pour des applications en tant que biomatériaux de régénération osseuse du fait de la présence des ions ortho- (briques élémentaires pour la formation d'apatite biologique) et d'ions pyrophosphate (hydrolysables en orthophosphates à pH acide ou par action d'enzymes présentes dans le tissu osseux). Ces matériaux sont élaborés par chimie douce incluant le passage d'une solution à un gel puis à un solide. L'objectif de cette thèse est d'étudier l'effet de différents paramètres de synthèse en vue de déterminer les mécanismes de formation de ces matériaux originaux et de décrire leur structure. En particulier, nous avons étudié l'effet de la composition relative de la solution initiale en précurseurs phosphates (différents ratios molaires en ions ortho- et pyrophosphates) et de la température de séchage lors de la dernière étape du protocole d'élaboration sur la composition et la structure du matériau final en mettant en œuvre différentes techniques de caractérisation complémentaires et à différentes échelles (DRX, MEB, MET, spectroscopie Raman, ATG/ATD, RMN du solide, analyses chimiques). Dans un premier temps, nous montrons qu'il est possible de contrôler la composition du matériau final à partir de celle de la composition de la solution initiale de précurseurs phosphates. Les compositions extrêmes étudiées (100% pyro- et 100% ortho-) conduisent à une phase plus ou moins bien cristallisée : la canaphite a et l'apatite nanocristalline, respectivement. En revanche, tous les matériaux élaborés à partir d'une solution de composition intermédiaire (mixte ortho- et pyro-) sont amorphes et leur composition a été déterminée ce qui a permis de proposer une formule chimique générale pour ces matériaux amorphes mixtes ortho et pyrophosphates de calcium et sodium hydratés. Il a été possible de préciser certaines hypothèses, comme l'effet inhibiteur de l'ion pyrophosphate sur la cristallisation de l'orthophosphate de calcium (et vice-versa), qui contrôle la structure du matériau final (amorphe ou cristallin) et ce pour une grande gamme de compositions de matériaux. La caractérisation fine par RMN du solide et par MET ont permis de mettre en évidence une formation de type colloïdale de ces types de matériaux et pouvant s'appuyer sur le modèle de cluster de type Posner. Dans un second temps, nous montrons que la température de séchage, est un paramètre contrôlant l'état d'hydratation de ces matériaux mais aussi la réaction d'hydrolyse interne des ions pyrophosphate en ions orthophosphate au sein du matériau ainsi que la réaction inverse de condensation des ions ortho- en ions pyrophosphate vers 400°C.[...]
The present work concerns the study of an amorphous hydrated calcium and sodium mixed ortho- and pyrophosphate materials which present an interest for biomaterial application in the case of bone regeneration due to the presence of orthophosphate ions (elementary brick of biological apatite formation) and pyrophosphate ions (hydrolysis phenomenon at low pH or by enzymatic activities in bone tissue). These materials are synthesis using soft chemistry protocol including a solution-gel transition and then to a solid state. The objective of this thesis is to study the effect of different synthesis parameters in order to determine the formation mechanism of the original materials and to describe their structure. In fact, we studied the effect of relative composition of initial solution on phosphate precursor (various ranges of molar ratios of ortho- and pyrophosphates) and the drying temperature effect during the last step of the synthesis protocol on the composition of the composition and the structure of the final material by using several complementary characterizations and at various scales (XRD, SEM, TEM, Raman spectroscopy, GTA/DTA, solid-state NMR and chemical analysis). Firstly, we showed that it is possible to control the composition of the final material using the composition of the initial phosphate precursor solution. The extreme compositions (100% pyro- and 100% ortho-) lead to a more or less crystallized phase such as a-canaphite and nanocrystalline apatite, respectively. On the other hand, all materials synthesised with an intermediate composition of phosphate precursor solution (mixed ortho- and pyro-) are amorphous and their compositions were used to propose a general chemical formula for theses amorphous hydrated calcium and sodium mixed ortho- and pyrophosphate materials. It has been possible to clarify certain hypotheses, such as the inhibitory effect of the pyrophosphate ions on the calcium orthophosphate crystallization (and vice-versa), which control the final structure of the material (amorphous or crystalline) and this control can be extended to a large range of composition. The solid-state NMR and the TEM highlight the colloidal formation of the materials and it can be relevant to compare them with Posner cluster model. In a second part, we showed that the drying temperature is a key parameter to control the hydration state of these materials but we can also use this parameter to understanding the internal hydrolysis reaction of the pyrophosphate ions into orthophosphate ions in the final material.[...]
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Dutilleul, Olivier. "Mécanisme de corrosion des verres P2O5-Na2O-CaO-M2O3 (M:Fe3+, Al3+) amorphes et dévitrifiés." Compiègne, 1992. http://www.theses.fr/1992COMPD491.

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Nous avons étudié la corrosion des verres de phosphate du système CaO-Na2O-P205 - MIII7O3 avecMIII = Fei+, AI3+. Après avoir effectué la préparation de ces verres nous nous sommes intéressés, dans une première étape, à l'étude structurale. L'étude du verre CaO-Na2O-P2O5, appelé PCN, montre que les chaînes de phosphate sont sous la forme de longues chaînes appelées métaphosphates. Si on augmente la teneur en Fe203, celui-ci devient modificateur de verre. Le fer dépolymérise alors le verre. Nous avons étudié ensuite la dévitrification des verres PCN xFe et PCNA xFe. Lorsqu'on ajoute de l'oxyde de Fer (Fe203) au verre PCN, la cristallisation passe alors par deux étapes avec la formation de phosphates doubles de sodium fer (orthophosphates) puis avec la formation des métaphosphates observés lors de la cristallisation des verres sans fer. La deuxième partie concerne la corrosion à 100°C en solution aqueuse des verres CaONa2O-P205-M203 avec M= Fe et/ou Al. Le profil de corrosion du verre PCN est identique à celui proposé par Bunker. Celui des verres dans lesquels on a ajouté du fer est différent. Le diffractogramme X du "gel" observé à la surface du verre PCN corrodé a montré que le phosphate de calcium était sous forme d'hydroxyapatite. Enfin nous avons proposé des modèles de corrosion différents pour PCN et PCN xFe. Le premier concerne PCN et fait intervenir une étape gel de métaphosphate puis une hydrolyse et une décondensation. Le deuxième mécanisme pour PCN xFe est plus complexe et varie suivant le taux de fer introduit. Pour des faibles pourcentages de fer, le mécanisme retenu est celui des chaînes formant un gel ; mais pour un taux élevé de fer, nous n'aurions pas au départ un gel de métaphosphate mais des hydrogels d'hydrogénophosphates puis ultérieurement des hydroxydes de fer colloïdaux qui en séchant ont donné de l'hématite.
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Rühl, Ralf. "Zur Calciumphosphatprazipitation mit Phosphoserin, Fetuin, Osteocalcin, Kollagen und in Vesikeln." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2011. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-78417.

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Der hierarchisch strukturierte und hoch geordnete Aufbau von Calciumphosphat und Kollagen in Knochen und Zähnen wird von den Zellen mit Hilfe bestimmter Moleküle erreicht. Diese organischen Moleküle, zumeist Proteine, beeinflussen durch die räumliche Anordnung ihrer Ladung das Präzipitations- und Wachstumsverhalten der mineralischen Phase. Die in dieser Arbeit beschriebenen Computersimulationen zeigen, dass ein Calciumphosphatkomplex mit deprotoniertem Phosphat am stabilsten ist. Vermutlich nimmt die Bindungsenergie pro Oberfläche des Komplexes mit wachsender Größe bis zu einem Ca9(PO4)6 -Komplex (Posner Klaster) linear zu. Die Präzipitation von Calciumphosphat aus wässriger Lösung führt häufig zu amorphen Kugeln mit 50-500 nm Durchmesser, die sphärische Unterstrukturen von ca. 5 nm Durchmesser zeigen und bei großer Dichte zu einer amorphen Schicht verschmelzen. Geringe Unterschiede in der Präparation können aber schon zu stäbchenförmigen oder plättchenartigen Kristalliten führen. Phosphoserin ist eine der wichtigsten Aminosäuren bei der Anbindung von Proteinen an Calciumphosphat. Das Computermodell zeigt an der gesamten Oberfläche dieser Aminosäure ein deutliches elektrisches Potential, dies begünstigt die Wechselwirkung mit Ionen. FT-IR- und NMR-Untersuchungen zeigen, dass Phosphoserin bei Kopräzipitation mit Calciumphosphat höchstwahrscheinlich in die mineralische Phase eingebaut wird. Serin zeigt bei der Kopräzipitation ab 1 mM einen Einfluss auf die Morphologie von Calciumphosphat, während Phosphoserin schon bei 0,01 mM einen deutlichen Einfluss zeigt. Elektronenspray-Ionisations-Massenspektroskopie (ESI-MS) bestätigt die relativ zum Serin intensivere Wechselwirkung von Phosphoserin mit Calciumphosphat. Das wichtigste Protein zur Vermeidung ektopischer Mineralisierung ist Fetuin. Dieses Protein stabilisiert die transient auftretenden amorphen Calciumphosphatkugeln (ACP-Kugeln) und erlaubt so dem Körper deren Entsorgung. Fetuin verhindert das Verschmelzen von ACP-Kugeln, wenn diese in großer Dichte auftreten, wobei deren feine Unterstruktur erhalten bleibt. Trotz des starken inhibitorischen Verhaltens wird das Auflösen von Brushit durch die Anwesenheit von Fetuin praktisch nicht beschleunigt. Auch auf die Kinetik der Assemblierung von Kollagen zeigt Fetuin praktisch keinen Einfluss. Des Weiteren wurde das Nukleationsverhalten des häufigsten, nichtkollagenen Knochenproteins, dem Osteocalcin (OC), mittels ESI-MS beobachtet. Die Untersuchungen von Osteocalcin in Calciumphosphatlösung zeigten Komplexe mit bis zu 8 Ca2+, der größte identifizierbare Komplex bestand aus [OC Ca2 (PO4 )2 Na4 ]+. Um die Mineralisierung von Kollagen genauer zu untersuchen, wurden assemblierte Kollagenfibrillen in der Flüssigzelle eines Atomkraftmikroskops (AFM) mit Calciumphosphat nachmineralisiert. Hierbei wurde eine gleichmäßige Anlagerung der offenbar amorphen mineralischen Phase beobachtet. Die Inkubation der Fibrillen mit Phospholipidvesikeln führte zu einem Aufweichen der Fibrillen. Des Weiteren wurden Phospholipidvesikel hergestellt, um den Calciumphosphatniederschlag in einem räumlich stark begrenzten Abschnitt zu untersuchen. Die Vesikel wurden mit REM und AFM abgebildet und so verschiedene Präparationsmethoden verglichen. Es konnten plättchenförmige Kristallite an der Vesikelmembran gezüchtet werden, während bei Anwesenheit von Phosphoserin globuläre Objekte auftraten. Eine Arbeitshypothese wurde entwickelt, die das unterschiedliche Wachstumsverhalten von Calciumphosphat in wässriger Lösung mit einer positiv geladenen Hydrathülle um den Calciumphosphatkeim erklärt. Die Protonen stammen vom deprotonierten Phosphat des Mineralkeims und können sich auf Grund der adsorbierten Wassermoleküle nicht sofort in der Lösung verteilen. Diese Hülle aus H3O+ verhindert das beliebige Anlagern von Ionen an den Mineralkeim und lenkt so dessen Morphologie.
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Wetherall, Kate M. "The Structure of Amorphous Calcium Phosphate and othe phosphate materials." Thesis, University of Kent, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.520863.

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Al-Lami, Mohammed Sabar. "Amorphous calcium phosphate nanocomplexes in dental film formulations." Thesis, University of Strathclyde, 2012. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=23885.

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The present thesis concerns the exploration of novel amorphous calcium phosphate nanocomplexes stabilised by food grade additives. The concept is to generate a formulation to prevent dental caries through enamel remineralisation, supplementing the calcium and phosphate concentrations by delivery from an adhesive dental film dosage form. A formulation containing calcium chloride, dibasic sodium phosphate and stabiliser in hydrochloric acid solution at a pH of 5 was complexed by pH adjustment to 7 in the presence of fractioned hydrolysed casein or negatively charged peptides. This allowed the formation of stabilised amorphous calcium phosphate nanocomplexes. An alternative method in which carboxymethyl cellulose with short chain length with measured molecular weight and degree of substitution, produced by acid hydrolysis, was similarly able to stabilise the amorphous calcium phosphate nanocomplex. The prepared amorphous calcium phosphate nanocomplexes were suitable for formulation in to dental film dosage forms. The prepared nanocomplexes were formulated into dental films based on hydroxy propyl methyl cellulose (HPMC) of various grades which formed hydrogels with good adhesive properties. The physical properties of the prepared films were characterised by texture analysis, tensile strength and structure and the calcium release was measured using film strips in a specially designed dissolution cell. The formulated dental films containing a mixture of 2% w/v E10M and 1% w/v F4M hydroxy propyl methylcellulose, loaded with 2 % w/v 4 hours acidic hydrolysed carboxy methylcellulose and hydrolysed casein, had a suitable release of calcium. The release pattern of the calcium was characterised using different diffusion/erosion models and was best described by a Higuchi mode with Fickian, non-Fickian and super case II transport kinetics depending on the type of ACP and the grade of HPMC used. The effect of a biofilm generated on the tooth surface to make the substrate more hydrophobic was examined. The adhesion of 15% (w/v) HPMC-E10M hydrogel to enamel slabs was studied, and was found to be highly variable. The effect of ACP on the culture of the biofilm using confocal laser scanning microscopy was investigated and a substantial inhibitory effect was obtained with 8% w/v ACP. The remineralisation effect of preparations containing 2% w/v NGP-ACP, HC-ACP and acid hydrolysed CMC-ACP loaded into films formulated with a mixture of 2% w/v E10M and 1% w/v F4M hydroxy propyl methylcellulose was investigated. The method employed used scanning electron probe microanalysis to determine the elemental deposition of the calcium and phosphorus up to a depth of 400μm in enamel samples. Both 2% w/v 4h ahCMC-ACP and 2% w/v HC-ACP containing dental films showed an uptake of calcium and phosphate with uniform and constant deposition of the elements to a depth of 400μm into the enamel samples.
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Simmons, Jane. "The structure and function of amorphous calcium phosphate biominerals." Thesis, University of Reading, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339520.

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Whited, Bryce Matthew. "Osteoblast Response to Zirconia-Hybridized Pyrophosphate Stabilized Amorphous Calcium Phosphate." Thesis, Virginia Tech, 2005. http://hdl.handle.net/10919/32699.

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Biodegradable polyesters, such as poly(DL-lactic-co-glycolic acid) (PLGA), have been used to fabricate porous bone scaffolds to support bone tissue development. These scaffolds allow for cell seeding, attachment, growth and extracellular matrix production in vitro and are replaced by new bone tissue when implanted into bone sites in vivo. Hydroxyapatite (HAP) and Æ Ã -tricalcium phosphate (Æ Ã -TCP) ceramics have been incorporated into PLGA bone scaffolds and have been shown to increase their osteoconductivity (support cell attachment). Although HAP, Æ Ã -TCP, and biodegradable polyesters are osteoconductive, there is no evidence that these scaffold materials are osteoinductive (support cell differentiation). Calcium and phosphate ions, in contrast, have been postulated to be osteogenic factors that enhance osteoblast differentiation and mineralization. Recently, a zirconia-hybridized pyrophosphate stabilized amorphous calcium phosphate (Zr-ACP) has been synthesized which permits controlled release of calcium and phosphate ions and thus is hypothesized to be osteoinductive. Incorporation of Zr-ACP into a highly porous poly(DL lactic-co-glycolic acid) (PLGA) scaffold could potentially increase the osteoinductivity of the scaffold and therefore promote osteogenesis when implanted in vivo. To determine the osteoinductivity of Zr-ACP, a MC3T3-E1 mouse calvarial-derived osteoprogenitor cell line was used to measure cell response to Zr-ACP. To accomplish this objective, Zr-ACP was added to cell culture at different stages in cell maturation (days 0, 4 and 11). DNA synthesis, alkaline phosphatase (ALP) activity, osteopontin synthesis and collagen synthesis were determined. Results indicate that culture in the presence of Zr-ACP significantly increased cell proliferation, ALP activity and osteopontin synthesis but not collagen synthesis. To determine the feasibility of incorporating Zr-ACP into a PLGA scaffold, PLGA/Zr-ACP composite foams (5% or 10% (w/v) polymer:solvent with 25 wt% or 50 wt% Zr-ACP) were fabricated using a thermal phase inversion technique. Scanning electron microscopy revealed a highly porous structure with pores ranging in size from a few microns to about 100 Æ Ã m. The amorphous structure of the Zr-ACP was maintained during composite fabrication as confirmed by X-ray diffraction measurements. Composite scaffolds also showed significantly greater compressive yield strengths and moduli as compared to pure polymer scaffolds. The results of this study indicate that Zr-ACP enhances the osteoblastic phenotype of MC3T3-E1 cells in vitro and can be incorporated into a porous PLGA scaffold. Porous PLGA/Zr-ACP composites are promising for use as bone scaffolds to heal bone defects.
Master of Science
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Popp, Jenni Rebecca. "Bioactive Poly(Lactic-co-Glycolic Acid)-Calcium Phosphate Scaffolds for Bone Tissue Regeneration." Diss., Virginia Tech, 2009. http://hdl.handle.net/10919/37558.

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Bone is currently the second most transplanted tissue, second only to blood. However, significant hurdles including graft supply and implant failure continue to plague researchers and clinicians. Currently, standard clinical procedures include autologous and allogeneic grafting. Autologous grafts may achieve functional repair; yet, they are available in limited supply and are associated with donor site morbidity. Allogeneic grafts are available in greater supply, but have a higher risk of infection. To overcome the disadvantages of current grafts, tissue engineering has become a major focus for the regeneration of bone. The goal of tissue engineering is to use a multidisciplinary approach to create biomimetic constructs that stimulate osteogenic regeneration to heal bone defects and restore tissue function. Biodegradable scaffolds are used in tissue engineering strategies as an interim template for tissue regeneration. The scaffold architecture provides mechanical support for cell attachment and tissue regeneration. Biocompatible poly(lactic-co-glycolic acid) (PLGA) has been processed through a number of techniques to create porous 3D architectures. Hydroxyapatite (HAP) and tricalcium phosphate have been used in conjunction with polymer scaffolds due to their osteoconductivity and biocompatibility, but they often lack osteoinductivity and are resistant to biodegradation. Conversely, amorphous calcium phosphate (ACP) is a mineral that solubilizes under aqueous conditions, releasing calcium and phosphate ions, which have been postulated to enhance osteoblast differentiation and mineralization. Controlled dissolution can be achieved by stabilizing ACP with divalent cations such as zinc or copper. Furthermore, incorporation of such osteogenic ACPs within a biodegradable PLGA scaffold could enhance the osteoconductivity of the scaffold while providing calcium and phosphate ions to differentiating osteoprogenitor cells, thereby stimulating osteogenesis when implanted in vivo. In this research, the effect of zinc on the differentiation of osteoprogenitor cells was investigated. Zinc supplementation of the culture media had no stimulatory effect on cell proliferation or differentiation. ACPs were synthesized using zirconium (ZrACP) and zinc (ZnACP) as stabilizers to achieve sustained ion release. Elevated concentrations suggested sustained ion release over the course of 96 hours and enhanced solubility of ZrACP and ZnACP. X-ray diffraction analysis showed a conversion of ZrACP to a semi-crystalline material after 96 hours, but ZnACP showed no conversion after 96 hours. Composite scaffolds were fabricated by incorporating HAP, zirconium-stabilized ACP (ZrACP), or zinc-stabilized ACP (ZnACP) into a sintered PLGA microsphere matrix and then characterized to determine the effect of the minerals on the in vitro differentiation of MC3T3-E1 cells. Scanning electron microscopy revealed a porous microsphere matrix with calcium phosphate powders distributed on the surface of the microspheres. Measurements of mechanical properties indicated that incorporation of 0.5 wt% calcium phosphates resulted in a 30% decrease in compressive modulus. When cells were cultured in the scaffolds, composite ACP scaffolds stimulated proliferation and ALP activity, while HAP scaffolds stimulated osteoblast gene expression. Overall, the results of this work indicate the addition of calcium phosphate minerals to PLGA scaffolds supported cell growth and stimulated osteogenic differentiation, making the scaffolds a promising alternative for bone tissue regeneration.
Ph. D.
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9

Mathieson, Robert. "Femtosecond laser modification of Fe3+ doped calcium phosphates." Thesis, University of Leeds, 2018. http://etheses.whiterose.ac.uk/22412/.

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Calcium phosphate based biominerals are the main inorganic constituent of bone and teeth. Therefore, the processing of these minerals provides a unique solution for a bioactive, structural material which can be used inside the body instead of typical bioinert materials. Dental enamel, a highly crystalline calcium phosphate, is prone to acid erosion and wear, which affects all ages, an early sign of which is hypersensitivity. Calcium phosphate minerals also play an important role in bone restoration and growth, and with an increased ageing population the number of hip and knee replacement revision surgeries are becoming more common. In this study, the main objective was to investigate the use of femtosecond pulsed lasers for sintering and processing of modified calcium phosphate minerals in order to analyse the efficacy of these methods for interfacial bonding and adhesion to titanium implant materials and dental enamel. Calcium phosphate minerals, specifically hydroxyapatite (Ca10(PO4)6OH2), were synthesised through the wet chemistry synthesis method, and were modified with the substitution of Fe3+ ions in various doping regimes. These materials were characterised via XRD, SEM, FTIR, Raman spectroscopy, UV-vis-NIR spectroscopy, particle size analysis and TGA/DSC. The following materials where then irradiated with near infrared sources in a variety of pulse repetition rates ranging from single shot to 1GHz. Ablation thresholds of the materials were found to remain relatively constant despite the addition of Fe3+ impurities into the structure. And working in lower repetition rate regimes, did not allow for successful sintering of the materials, however, it could be bonded to the titanium substrates relatively in this regime. Higher repetition rates regimes allowed for reasonable heat accumulation in the material which then generated more successful sintering and bonding onto dental enamel surfaces.
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Layrolle, Pierre. "Synthèse de phosphates de calcium en milieu organique." Toulouse, INPT, 1994. http://www.theses.fr/1994INPT041G.

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Les phosphates de calcium presentent un grand interet tant fondamental qu'applique puisqu'ils constituent la partie minerale des tissus calcifies des vertebres. Les principales methodes de synthese par precipitation en milieu aqueux ainsi que la nature chimique et cristallographique de ces composes sont presentees dans un premier chapitre bibliographique. L'accent est mis sur les recentes recherches visant a precipiter ces solides dans un milieu mixte eau-solvant organique. Dans le but de synthetiser des phosphates de calcium en milieu totalement organique, le second chapitre est consacre au choix du solvant et des reactifs les plus appropries. Le choix du diethylate de calcium comme precurseur organometallique et de l'ethanol comme solvant est defini. Dans le troisieme chapitre les precipites obtenus lors de la neutralisation de l'acide orthophosphorique par le diethylate de calcium dans l'ethanol sont etudies. Suivant les proportions de ces reactifs, leur rapport ca/p varie de 0,50 a 1,66. Ils sont amorphes et l'analyse de leur evolution thermique a montre la cristallisation de phases metastables des 700c comme le beta pyrophosphate, l'apha tcp, ou l'oxyapatite. Leur microstructure est etudiee par meb et par diffusion des rx. L'addition d'ions hydroxyde, fluorure, chlorure ou carbonate permet la coprecipitation de phosphates de calcium substitues. La synthese de ces nouveaux solides amorphes et leur cristallisation thermique en apatite stoechiometrique ca10(po4)6(x)2 avec x=oh, f, cl et o, fait l'objet d'un quatrieme chapitre. Enfin, la potentialite de la methode de synthese est particulierement bien illustree par la coprecipitation de composes mineral-organiques, etudiee dans le dernier chapitre. Ces materiaux hybrides sont obtenus par addition dans le milieu, de monoethyl phosphate pur. Leur etude par spectroscopie ir, rmn du solide et meb confirme l'existence d'une solution solide entre un phosphate mineral et organique
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Books on the topic "Phosphates de calcium amorphes"

1

Calcium phosphates in oral biology and medicine. Basel: Karger, 1991.

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Amjad, Zahid, ed. Calcium Phosphates in Biological and Industrial Systems. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-5517-9.

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Structure and chemistry of the apatites and other calcium orthophosphates. Amsterdam [The Netherlands]: Elsevier, 1994.

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Dorozhkin, Sergey V. Calcium orthophosphate-based biocomposites and hybrid biomaterials. Hauppauge, N.Y: Nova Science Publishers, 2009.

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Calcium orthophosphates: Applications in nature, biology, and medicine. Singapore: Pan Stanford, 2012.

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International Workshop on Phosphate and Other Minerals (7th 1985 Marseille, France). Phosphate and mineral homeostasis. New York: Plenum Press, 1986.

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Thin calcium phosphate coatings for medical implants. New York: Springer, 2009.

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Felix, Bronner, and Peterlik Meinrad, eds. Cellular calcium and phosphate transport in health and disease: Proceedings of the Third International Workshop on Calcium and Phosphate Transport Across Biomembranes, held in Vienna, Austria, March 1-4, 1987. New York: Liss, 1988.

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Meinrad, Peterlik, and Bronner Felix, eds. Molecular and cellular regulation of calcium and phosphate metabolism: Proceedings of the Symposium on Molecular and Cellular Regulation of Calcium and Phosphate Metabolism, held in Vienna, November 17, 1988. New York: Wiley-Liss, 1990.

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Dorozhkin, Sergey V. Hydroxyapatite and Other Calcium Orthophosphates: Nanodimensional, Multiphasic and Amorphous Formulations. Nova Science Publishers, Incorporated, 2017.

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Book chapters on the topic "Phosphates de calcium amorphes"

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Eanes, Edward D. "Amorphous Calcium Phosphate: Thermodynamic and Kinetic Considerations." In Calcium Phosphates in Biological and Industrial Systems, 21–39. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-5517-9_2.

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LeGeros, Racquel Z., Dindo Mijares, J. Park, X. F. Chang, I. Khairoun, Regina Kijkowska, Renata Dias, and John P. LeGeros. "Amorphous Calcium Phosphates (ACP): Formation and Stability." In Bioceramics 17, 7–10. Stafa: Trans Tech Publications Ltd., 2005. http://dx.doi.org/10.4028/0-87849-961-x.7.

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Aimanova, O. J., Racquel Z. LeGeros, and V. A. Sinyayev. "Antimicrobiologic Property Hydrated Amorphous Calcium Phosphates Containing Silver." In Bioceramics 17, 439–44. Stafa: Trans Tech Publications Ltd., 2005. http://dx.doi.org/10.4028/0-87849-961-x.439.

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Eanes, E. D. "Amorphous Calcium Phosphate." In Monographs in Oral Science, 130–47. Basel: KARGER, 2001. http://dx.doi.org/10.1159/000061652.

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Dorozhkin, Sergey V. "Calcium Phosphates." In Handbook of Bioceramics and Biocomposites, 91–118. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-12460-5_9.

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Dorozhkin, Sergey V. "Calcium Phosphates." In Handbook of Bioceramics and Biocomposites, 1–22. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-09230-0_9-1.

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Elliott, James C. "11. Calcium Phosphate Biominerals." In Phosphates, edited by Matthew J. Kohn, John Rakovan, and John M. Hughes, 427–54. Berlin, Boston: De Gruyter, 2002. http://dx.doi.org/10.1515/9781501509636-014.

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Chow, L. C. "Solubility of Calcium Phosphates." In Monographs in Oral Science, 94–111. Basel: KARGER, 2001. http://dx.doi.org/10.1159/000061650.

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Okano, K., H. Ohtake, M. Kunisada, H. Takano, and M. Toda. "Phosphate Recovery Using Amorphous Calcium Silicate Hydrates." In Phosphorus Recovery and Recycling, 435–47. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-8031-9_30.

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Berridge, Michael J. "Inositol phosphates and calcium signaling." In Peptides, 297–301. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-010-9595-2_90.

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Conference papers on the topic "Phosphates de calcium amorphes"

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Terebilenko, A., K. Terebilenko, N. Slobodyanik, and E. Zubar. "Synthesis and luminescence properties of calcium phosphates." In 2014 IEEE International Conference on Oxide Materials for Electronic Engineering (OMEE). IEEE, 2014. http://dx.doi.org/10.1109/omee.2014.6912394.

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Sun, L., C. C. Berndt, R. S. Lima, A. Kucuk, and K. A. Khor. "Effects of Spraying Parameters on Phase Formation and Distribution in Plasma-Sprayed Hydroxyapatite Coatings." In ITSC 2000, edited by Christopher C. Berndt. ASM International, 2000. http://dx.doi.org/10.31399/asm.cp.itsc2000p0803.

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Abstract Calcined spray-dried hydroxyapatite (Ca10(PO4)(OH)6; i.e., HA) powders were atmospherically plasma sprayed (APS) using various process parameters. The resulting phases within the coating surface and the interface between the coating and the substrate were analyzed using X-ray diffraction (XRD) methods. This XRD revealed the presence of both amorphous (i.e., amorphous calcium phosphate: ACP) and crystalline phases. The crystalline phases included both HA and some impurity phases from the decomposition of HA, such as tricalcium phosphate (α-TCP and β-TCP), tetracalcium phosphate (TTCP) and calcium oxide (CaO). The crystallinity of HA decreased with increasing spray power and stand-off distance (SOD). The percentage of all impurity phases increased with the spray power. The percentage of both TCP and TTCP decreased with the SOD while the CaO percentage increased. In addition, the percentage of ACP and CaO were higher in the interface than at the surface of the coating while the percentage of TCP and TTCP exhibited the opposite effect.
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Setijanto, Darmawan, Nilna Nur Putri, Taufan Bramantoro, Titiek Berniyanti, Agung Sosiawan, Retno Palupi, and Gilang Rasuna Sabdho Wening. "Casein Phosphopeptide-Amorphous Calcium Phosphate Fluoride (CPP-ACPF) as an Enamel Remineralization." In The 7th International Meeting and The 4th Joint Scientific Meeting in Dentistry. SCITEPRESS - Science and Technology Publications, 2017. http://dx.doi.org/10.5220/0007291200170021.

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Rizzi, Rosanna, Angela Altomare, Nicola Corriero, Aurelia Falcicchio, and Corrado Cuocci. "Physico-Chemical Characterization of New Rare-Earth Doped Calcium Phosphates." In The 2nd International Online Conference on Crystals. Basel, Switzerland: MDPI, 2020. http://dx.doi.org/10.3390/iocc_2020-07737.

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Asadi-Eydivand, Mitra, Amir Hossein Hakami Vala, Arghavan Farzadi, Soheila Ali Akbari Ghavimi, and Mehran Solati-Hashjin. "Using an artificial intelligence technique to optimize calcium phosphates synthesis conditions." In 2012 IEEE EMBS Conference on Biomedical Engineering and Sciences (IECBES 2012). IEEE, 2012. http://dx.doi.org/10.1109/iecbes.2012.6498107.

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Sedelnikova, M. B., E. G. Komarova, Yu P. Sharkeev, and T. V. Tolkacheva. "Formation and properties of biocoatings based on wollastonite and calcium phosphates." In ADVANCED MATERIALS WITH HIERARCHICAL STRUCTURE FOR NEW TECHNOLOGIES AND RELIABLE STRUCTURES. AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4932892.

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Chakraborty, Rajib, Susmita Datta, Mohammad Shahid Raza, and Partha Saha. "Improvement of Ionic Bonding Strength and Electrochemical Corrosion Resistance of Hydroxyapatite- Calcium Phosphate Pulsed Electrochemically Deposited In-Situ Coating Through Hydroxyl Ion Treatment." In ASME 2018 13th International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/msec2018-6582.

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Hydroxyl ion treatment of different hydroxyapatite-calcium hydrogen phosphate composite in-situ coatings synthesized through pulsed electro-deposition with varying amount of hydroxyapatite phase and degree of crystallinity were carried out with the help of highly basic solution in order to achieve a more chemically stable and corrosion resistance performance under contact with body fluid. The coatings exhibit altogether completely different behaviour in terms of bond formation, surface topography generation, phase transformation and corrosion behaviour. Detailed characterizations of formed top surface layer were carried out with the help of XRD, SEM and FTIR in order to correlate the results with their base surface characteristics. Transformation of <020> and <121> surface parallel planes of calcium hydrogen phosphate in to <002> and <112> planes of hydroxyapatite took place in all the coatings along with formation of nano-crystalline structure. Calcium-rich porous hydroxyapatite scaffold formation takes place in low current density coating which in general exhibits low stability in terms of chemical bonding strength vis-à-vis corrosion protection performance. 10 mA/cm2 coating, which come with optimum presence of hydroxyapatite phase and crystallinity post electro-deposition, showed significant improvement in terms of increasing hydroxyl and phosphate bond polarization strength of hydroxyapatite phase and the same lead to improvement in the overall corrosion resistance performance of the coating by two times. Despite of formation of highest amount of hydroxyapatite phase during hydroxyl ion treatment in 20 mA/cm2 coatings, the corrosion protection performance results are negative on account of dilution of mostly low bonding amorphous phases with high internal residual stress.
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Jha, A., E. Elmadani, G. Jose, C. G. Leburn, C. T. A. Brown, W. Sibbett, M. S. Duggal, and J. Toumba. "Pulsed laser sintering of photo-active calcium phosphates for dental enamel restoration." In 12th European Quantum Electronics Conference CLEO EUROPE/EQEC. IEEE, 2011. http://dx.doi.org/10.1109/cleoe.2011.5943233.

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"COLLIMATION OF A SPUTTERED FLUX DURING THE GLANCING ANGLE DEPOSITION OF CALCIUM PHOSPHATES." In Fizicheskaya mezomekhanika. Materialy s mnogourovnevoy ierarkhicheski organizovannoy strukturoy i intellektual'nye proizvodstvennye tekhnologii. Tomsk State University, 2020. http://dx.doi.org/10.17223/9785946219242/286.

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Ranz, X., T. Aslanian, L. Pawlowski, L. Sabatier, and R. Fabbro. "Phases Transformations in Laser Treated Hydroxyapatite Coatings." In ITSC 1998, edited by Christian Coddet. ASM International, 1998. http://dx.doi.org/10.31399/asm.cp.itsc1998p1343.

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Abstract The hydroxyapatite Ca10(PO4)6OH2 (HAP) was plasma sprayed onto titanium alloy substrate. The samples having thickness of about 150 µm ware sprayed in a way to obtain two different content of crystalline HAP: 25 an 30 %. The coatings ware subsequently submitted to laser treatment with the CO2 laser. The treatment was carried out with different laser powers and scanning velocities and resulting thereof sample surface temperatures and the kinetics of the thermal fields ware monitored with a pyrometer. The XRD method enabled verification of the crystallinity state of HAP, content of amorphous calcium phosphate and the content of foreign phases. Optical microscope was used to check the microstructure and the depth of laser modified zone.
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