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Relevant bibliographies by topics / Phosphopeptide enrichment / Dissertations / Theses

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Dissertations / Theses on the topic 'Phosphopeptide enrichment'

Author: Grafiati

Published: 4 June 2021

Last updated: 17 February 2022

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1

Batalha, Íris Cristina da Luz. "Engineered structures for the profiling and enrichment of the phosphoproteome." Doctoral thesis, Faculdade de Ciências e Tecnologia, 2014. http://hdl.handle.net/10362/13107.

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Dissertação para obtenção do Grau de Doutor em Bioengenharia (MIT-Portugal)<br>O capítulo 1 foi parcialmente reproduzido de um artigo previamente publicado sob permissão dos editores originais e sujeito às restrições de cópia impostos pelos mesmos.<br>Fundação para a Ciência e a Tecnologia - (SFRH/BD/64427/2009) and the project PTDC/EBB-BIO/102163/2008 assigned to Prof. Cecília Roque, and also to the Associate Laboratory REQUIMTE (PEst-C/EQB/LA0006/2013)

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2

Ewen-Al-Kashi, Sally. "Functional group imprinted polymers : towards a novel method of phosphopeptide enrichment using molecular imprinting technology." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/18943.

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A range of techniques exists for the characterisation of the phosphoproteome, the most effective of which is mass spectrometry (MS); however, enrichment of phosphorylated peptides is a necessary prerequisite. Current enrichment strategies are inadequate, either due to low selectivity or because they involve chemical modification of the sample. Adapting molecular imprinting technology, we have designed and synthesised a series of prototype synthetic phospho-receptors, termed "Functional Group Imprinted Polymers" (FIPs). We define 'FIPs' as imprinted polymers that selectively recognise molecules

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3

Haaf, Erik. "Qualitative und quantitative Analyse der Phosphorylierung von Proteinen der circadianen Uhr." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2012. http://dx.doi.org/10.18452/16578.

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Die Phosphorylierung als posttranslationale Modifikation von Proteinen spielt bei Signalwegen in Zellen eine große Rolle. Für die Entschlüsselung des molekularen Mechanismus der circadianen Uhr ist es daher von Interesse, die Phosphorylierungsstellen beteiligter Proteine zu identifizieren. Im Rahmen dieser Arbeit wurden die Proteine Period I und II mittels Flüssigkeitschromatographie und Tandemmassenspektrometrie (LC-MS/MS) auf Phosphorylierungsstellen untersucht. Hierbei lag der Fokus auf der Verbesserung bestehender Methoden, um eine bessere und umfassendere Identifizierung der Phosphoryli

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4

Dunn, Jamie D. "Polymer-modified plates for enrichment of phosphopeptides prior to analysis by matrix-assisted laser desorption/ionization mass spectrometry." Diss., Connect to online resource - MSU authorized users, 2007.

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5

Subedi, Prabal [Verfasser], Katrin [Gutachter] Marcus, and Dirk [Gutachter] Wolters. "Use of molecularly imprinted polymers for enrichment of phosphopeptides and alpha-Synuclein, and the mass spectrometric analyses of alpha-Synuclein in \(\it post-mortem\) human brains / Prabal Subedi ; Gutachter: Katrin Marcus, Dirk Wolters." Bochum : Ruhr-Universität Bochum, 2016. http://d-nb.info/1123283117/34.

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6

Ke, Juin-Yi, and 柯鈞翊. "Facile Preparation of Metal Oxide/Polyarginine Hybrids and their Application in Phosphopeptide Enrichment." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/78643919483293346814.

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碩士<br>國立暨南國際大學<br>應用化學系<br>102<br>Here we report a rapid and facile approach for synthesizing metal oxide hybrids material. Polyarginine-coated Nanodiamonds (PAND) were utilized as a multiplex loading platform to accommodate various metal oxides (MOx) and developing them into new materials containing two different metals. We have estimated the absorption of MOx for PAND by measuring the particle size in different ratio. MOx/PA-ND was feasible to compositing two or more different metal oxide onto PAND, and it can also controlled unequal contents of different metal oxides and confirm by EDS anal

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7

Fíla, Jan. "Úloha fosforylace proteinů v progamické fázi vývoje samčího gametofytu tabáku." Doctoral thesis, 2016. http://www.nusl.cz/ntk/nusl-341981.

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v angličtině (English abstract) Tobacco male gametophyte has a strongly dehydrated cytoplasm and represents a metabolically inactive stage. Upon cytoplasm rehydration, pollen grain becomes metabolically active and after the activation is finished, the pollen tube growth through a selected pollen aperture starts. The rehydration together with metabolic activation are accompanied by the regulation of translation and post-translational modifications (mainly phosphorylation) of the existing proteins. In this Ph.D. thesis, there were identified phosphopeptides from tobacco (Nicotiana tabacum) matur

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8

Zeng, Yi-You, and 曾乙祐. "Mesoporous TiO2 Nanowires for Selective and Reproducible Enrichment of Phosphopeptides." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/28519707295374520955.

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碩士<br>國立暨南國際大學<br>應用化學系<br>100<br>Abstract Titanium dioxide (TiO2) affinity enrichment has been widely used in phosphoproteomic studies. Although many attempts have been made to fabricate TiO2-based materials for selective enrichment of phosphopeptides, the effects of structural properties, such as crytsallinity, pore diameter, porosity, and specific surface area of TiO2, on selectivity has not been widely explored. Herein, we synthesized TiO2 nanowires with well-defined nanopores and high specific surface area for selective and reproducible enrichment of phosphopeptides from complex peptide m

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9

YE, YU-YANG, and 葉羽揚. "Preparation of Zirconia Hollow Nanocage for Specific Enrichment of Phosphopeptides." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/947887.

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碩士<br>國立暨南國際大學<br>應用化學系<br>107<br>In this work, we have developed a novel synthesis strategy for the production of ZrO2 hollow nanocage(ZrO2 HC)for highly efficient enrichment of phosphopeptides via a Nanodiamond(ND)-assisted template method. We coated the silica dioxide and zirconium dioxide on the ND by sol-gel method, and the ND template was removed by calcination. The nanostructure of the obtained ZrO2 hollow nanocage was characterized by nitrogen adsorption-desorption isotherms, energy-dispersive X-ray spectroscopy(EDS),transmission electron microscopy (TEM). The obtained ZrO2 HC exhibite

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10

Lu, Jing-Wun, and 盧敬文. "Facile Synthesis of Titanium Sulfonate-Functionalized Nanodiamonds for Selective Enrichment of Phosphopeptides." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/3889xh.

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博士<br>國立暨南國際大學<br>應用化學系<br>105<br>In this work, we have developed a novel synthesis route for the production of titanium (IV) sulfonate-functionalized nanodiamonds (Ti4+-SND) as Ti4+-IMAC (Immobilized metal-ion affinity chromatography)adsorbents for selective enrichment of phosphopeptides. Nanodiamonds were first functionalized with polyarginine via EDC-mediated coupling reaction and then the titanium-sulfonate functionalization was performed by mixing with polystyrene sulfate and TiCl4 solutions. The obtained Ti4+-SNDs were used to specific capture of phosphopeptides from standard protein dig

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11

Hung, Shain-Un, and 洪翔文. "Selective Enrichment of Phosphopeptides from HeLa cell lysate using Titanium Dioxide-coated Nanodiamonds." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/86913670871509553250.

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碩士<br>國立暨南國際大學<br>應用化學系<br>101<br>Selective isolation and concentration of phosphopeptides is a prerequisite step prior to MS analysis. Currently titanium oxide affinity chromatography has gained more and more popularity among the common phosphopeptide isolation methods. Here, we present a new hybrid material, TiO2-coated nanodiamonds (TiO2-NDs), for selective enrichment of phosphopeptides from complex peptide mixtures. The results showed that TiO2-NDs outperformed the commercial TiO2 beads for higher enrichment capacity and specificity toward phosphopeptides. We identified 932 unique phos

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12

Chen, Huang-juo, and 陳煌焯. "Functionalized carbon encapsulated iron nanoparticles as affinity probes for enrichment of phosphoproteins /phosphopeptides." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/93544464646933115029.

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碩士<br>國立暨南國際大學<br>應用化學系<br>97<br>Abstract Carbon encapsulated magnetic nanoparticles have wide applications such as, serving as contrast reagents for magnetic resonance imaging (MRI), magnetic data storage medium, and magnetic hyperthermia. However, compared to the intense studies in the synthetic strategies for fabricating such core/shell materials, little effort has been devoted to the surface functionalziation strategies. In the report, we produced polyarginine-immobilized and titanium dioxide-immobilized carbon encapsulated magnetic iron nanoparticles (Fe@CNPs) as high affinity probes to e

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13

Chen, Yu-Wei, and 陳昱瑋. "Sequential Enrichment of Glycopeptides and Phosphopeptides by Zwitterionic-HILIC (ZIC-HILIC) Magnetic Nanoparticles." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/njd454.

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碩士<br>國立中央大學<br>化學學系<br>106<br>Among various protein post-translational modification (PTM), N-linked glycosylation and phosphorylation are among the most ubiquitous PTMs and play important roles in many biological functions, such as signal transduction, protein stability, and mobility. However, due to inherent heterogeneity of modification site and structure and ion suppression effect from free peptides during mass spectrometry analysis, it is necessary to develop enrichment strategy prior to mass spectrometry (MS) analysis. Though there are many enrichment methods developed for these two PTMs

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14

HUANG, CHIEN-YU, and 黃千瑜. "Selection enrichment and detection of phosphopeptides using zirconium ion and polyarginine functionalized nanodiamonds." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/92641123990999522148.

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碩士<br>國立暨南國際大學<br>應用化學系<br>104<br>In this work, we have developed a facile method for efficient production of metal-ion and polyarginine co-functionalized nanodiamonds （denotes as PA-ND@PSS-Mn+） as a novel IMAC platform for phosphoproteomic analysis. Polyarginine-coated nanodiamonds （PA-ND） were mixed with poly-styrenesulfonate（PSS） via multivalent electrostatic interactions. Immobilization of zirconium ion on the surface of poly-arginine/poly-styrenesulfonate（PSS） functionalized nanodiamonds was done by a 30-min incubation in ZrCl4 solution at room temperature. The PA-ND@PSS-Zr4+　was furth

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15

HSIAO, CHEN-FANG, and 蕭争芳. "Preparation of TiO2 Hollow Nanocages for Highly Selective Enrichment of Phosphopeptides by Nanodiamond-based Template Method." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/b3q3f8.

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碩士<br>國立暨南國際大學<br>應用化學系<br>106<br>In this work, we have developed a novel synthesis strategy for the production of TiO2 hollow nanocage for highly efficient enrichment of phosphopeptides via a Nanodiamond(ND)-assisted template method. We reduced the ND surface to OH groups and then coated the silica dioxide and titanium dioxide on the ND by sol-gel method, and the ND template was removed by calcination at 650 degrees for eight hours. The nanostructure of the obtained TiO2 hollow nanocage was characterized by nitrogen adsorption-desorption isotherms, X-ray powder diffraction (XRD), transmission

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