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Wibowo, Sulistiyo. "‘OVERLOAD’ POTRET TRANSPORTASI ANGKUTAN BARANG DALAM KARYA STREET PHOTOGRAPHY." Jurnal Ilmiah Publipreneur 9, no. 1 (July 1, 2021): 16–29. http://dx.doi.org/10.46961/jip.v9i1.183.
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Humans are creatures that are able to move and move from one place to another. Long before the wheel was discovered as part of the means of human transportation, animals and livestock such as horses, cows, camels and even elephants were used as a means of transportation. Apart from being a tool to speed up and lighten the travel time of these animals, sometimes the burden is attached to the rider. After the wheels were found, humans made transport vehicles that functioned as places for passengers and goods which were then pulled by animals so that the loads carried became more efficient and more efficient. Along with the development of time from time to time until finally motorized vehicles were found, the distance between the movement of people and goods was getting farther, more numerous and faster. Humans can move freely with current road facilities and adequate facilities. Almost everyone today can afford a vehicle from a bicycle to a plane depending on the abilities and needs of each individual. Movement or mobilization is not only human but also goods. Movable goods clearly require transportation assistance and humans as the perpetrators and are supported by the ease of road facilities and infrastructure. Each vehicle created has its own specifications and specifications, be it designation, capacity and recommended load. For example, passenger vehicles are not allowed to carry goods and vice versa. Especially if it is related to the recommended load capacity, whether it is a passenger vehicle or goods, it will clearly affect the safety of both the driver, passengers and luggage. Seeing the tendency in Indonesia, things related to the transportation function and discipline are often violated, the writer and photographer in this study is interested in the theme of 'overload', namely the portrait of transportation, especially freight transport in Indonesia, which is often seen carrying excess burden or over capacity in street / Street photography works. Photography Keywords: Street Photography, Transportation, Street Photography
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HAYASHI, Takashi, and Dai HANAJIMA. "An Analysis on Socio-Spatial Behavior of Dairy Cows Herd by Oblique Aero-Photography Method." Nihon Chikusan Gakkaiho 69, no. 3 (1998): 299–305. http://dx.doi.org/10.2508/chikusan.69.299.
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Kwaśnicki, R., A. Dobicki, A. Zachwieja, and P. Nowakowski. "Interrelationship between thermal imaging data and dairy traits in red-and-white cows." Biotehnologija u stocarstvu 23, no. 5-6-1 (2007): 277–82. http://dx.doi.org/10.2298/bah0701277k.
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The aim of the research was the thermal imaging comparative analysis of milk production traits such as: CFU (number of micro-organism forming colonies ?1 cm-3 of milk), somatic cells count - SCC, content (%) of fat, protein, lactose, DM, no fat dry matter - NFDM and urea (mg?100 cm-3) and the skin temperature at the selected sites on a cow?s body. The research was conducted on 32 cows of Red-and-White breed, with an average milk yield 6797 kg per cow. On two sides of the body (left and right) two fixed temperature measurement sites on the joints (ankle joint, carpal joint) and 3 sites on the mammary glands (fore quarter teat cistern, hind quarter teat cistern and central ligament) were chosen as well as isotherm was drawn for the temperature distribution along the oblique body axis to record the minimal and maximal skin temperatures. Two sessions of thermal imaging photography 4 to 5 hours after morning milking were completed. An analysis demonstrated that the minimum temperatures along oblique body axis of clinically healthy cows measured on left body side relate with SCC (r = - .788; P? 0.05) and milk fat and DM contents (r = +.428 and r = +.450; P? 0.05). Temperature of the skin covering udder central ligament was related negatively with % content of milk fat and protein (r = -.438 and r = -.442; P?0.05 - for the left body side).
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Banerjee, Sandeep. "“NOT ALTOGETHER UNPICTURESQUE”: SAMUEL BOURNE AND THE LANDSCAPING OF THE VICTORIAN HIMALAYA." Victorian Literature and Culture 42, no. 3 (June 6, 2014): 351–68. http://dx.doi.org/10.1017/s1060150314000035.
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During his third expedition into the higher Himalaya in 1866, the most ambitious of his three journeys into the mountains, Samuel Bourne trekked to the Gangotri glacier, the source of the Ganges. At that site he took “two or three negatives of this holy and not altogether unpicturesque object,” the first photographs ever made of the glacier and the ice cave called Gomukh, meaning the cow's mouth, from which the river emerges (Bourne 96). These words of Victorian India's pre-eminent landscape photographer, importantly, highlight the coming together of the picturesque mode and the landscape form through the medium of photography. In this essay, I focus on Samuel Bourne's images of the Himalaya, produced between 1863 and 1870, to query the ideological power of this triangulation to produce a specific image of the mountains in late nineteenth-century Victorian India. Situating Bourne's images in relation to contemporaneous material practices of the British within the space of the Himalaya, namely, the establishment of hill stations as picturesque locales in the higher altitudes of the Indian subcontinent, I argue that the landscape form, the picturesque mode, and the photographic medium, inflect each other to tame the sublimity of the mountains by representing them as similar to the Alps.
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Sett, Alisha, and Kajri Jain. "What Renders the Master’s House Unrecognizable? An Interview with Kajri Jain." Master, Vol. 5, no. 2 (2020): 96–105. http://dx.doi.org/10.47659/m9.096.int.
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I spoke with Kajri Jain over Zoom during the early days of the pandemic in 2020. Our conversation began with a discussion of her early fieldwork in the bazaars in India, probing into Jain’s own education and formative experiences. It then detoured into a critical unpacking of art history’s “sacred cows’, the need to fundamentally rethink the discipline’s deep intertwining with colonialism, and the many forms of baggage that non-Western art historians must carry on their shoulders. Jain’s suspicion of medium specific approaches led to a productive dialogue about anthropologist Michael Taussig’s work, theory fetishism, and several facets of contemporary photography in South Asia. We agreed about the need to continue to critique an elitist discourse that misunderstands the importance of religion, and the embedded nature of caste, in any reading of aesthetics and mass culture in the subcontinent. Ending with the question of how to decolonize, provincialize and globalize when engaged in pedagogy, Jain left us with much to contemplate. Keywords: visual anthropology, art history and decolonialization, Indian aesthetics, secularism and religion
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Linkman, A. E. "The Workshy Camera : Photography and the Labouring Classes in the Nineteenth Century." Costume 25, no. 1 (January 1, 1991): 36–52. http://dx.doi.org/10.1179/cos.1991.25.1.36.
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Pinto, Severino, Gundula Hoffmann, Christian Ammon, Wolfgang Heuwieser, Harel Levit, Ilan Halachmi, and Thomas Amon. "Effect of Two Cooling Frequencies on Respiration Rate in Lactating Dairy Cows Under Hot and Humid Climate Conditions." Annals of Animal Science 19, no. 3 (July 1, 2019): 821–34. http://dx.doi.org/10.2478/aoas-2019-0026.
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AbstractThe aim of this study was to evaluate the effects of evaporative cooling at two different frequencies per day on the respiration rate (RR) of lactating dairy cows, considering cow-related factors. Twenty multiparous Israeli Holstein dairy cows housed in a naturally ventilated cowshed were divided randomly into two treatment groups. The cows of both groups were exposed to 3 or 8 cooling sessions per day (3xcool vs. 8xcool, respectively). The RR was observed hourly, with a maximum of 12 measurements per day. Body posture (standing vs. lying) was simultaneously documented. Milk yield was recorded daily. Coat color was determined from a digital photograph. The RR of standing and lying cows was lower in the 8xcool group (60.2 and 51.6 breaths per min (bpm), respectively) than in the 3xcool group (73.1 and 65.6 bpm, respectively). For each increment of five kilograms of milk produced, RR increased by one bpm, and the RR of cows in early days in milk (DIM) was 12.3 bpm higher than that of cows in late DIM. In conclusion, eight cooling sessions per day instead of three lead to a RR abatement in heat-stressed cows under hot conditions, and cow-related factors directly impact the RR during heat stress assessment.
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Battini, Monica, Anna Agostini, and Silvana Mattiello. "Understanding Cows’ Emotions on Farm: Are Eye White and Ear Posture Reliable Indicators?" Animals 9, no. 8 (July 24, 2019): 477. http://dx.doi.org/10.3390/ani9080477.
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Understanding the emotions of dairy cows is primarily important in enhancing the level of welfare and provide a better life on farm. This study explored whether eye white and ear posture can reliably contribute to interpret valence and arousal of emotions in dairy cows. The research was conducted in five Italian dairy farms. Four hundred and thirty-six photographs of cows’ heads were scored (four-level), according to the eye white and ear posture during feeding, resting, pasture, and an avoidance distance test at the feeding rack (ADF test). Eye white and ear posture were significantly correlated and influenced by the context (P = 0.001). Pasture was the most relaxing context for cows (67.8% of half-closed eyes; 77.3% ears hung down or backwards). The excitement during ADF test was high, with 44.8% of eye white being clearly visible and ears directed forwards to the approaching assessor (95.5%). Housing and management mostly influenced emotions during feeding and resting (P = 0.002 and P = 0.001, respectively): where competition for feeding places and cubicles was low, the cows showed the highest percentages of half-closed eyes and ears backwards or hung down. This research supports the use of eye white and ear posture as reliable indicators of emotions in dairy cows.
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Harris, H. S. "The Cows in the Dark Night." Dialogue 26, no. 4 (1987): 627–44. http://dx.doi.org/10.1017/s0012217300018217.
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In the far-off days before the first World War, the British journal Mind was full of articles by writers who thought of themselves as “Neoidealists”. So when the enfant terrible of the groves of Academic Oxford in that generation—a “pragmatic Humanist” by the name of Ferdinand Canning Scott Schiller—played his most notorious practical joke upon his colleagues by publishing a mock-issue of the journal (under the title Mind!) he offered as a frontispiece “A portrait of the Absolute in the pink of condition”. Beneath a pale-pink semi-transparent tissue (which, except for its colour, was quite normal for the photographic plates in Victorian memoirs) one found a printed frame that embraced a perfectly blank white sheet of paper.
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Bready, Jennifer, and Sharon Sterken. "Polynomials on Pictures with Different Platforms." Mathematics Teacher 111, no. 2 (October 2017): 144–48. http://dx.doi.org/10.5951/mathteacher.111.2.0144.
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When students are taught to graph functions, they typically are taught using a graphing calculator. But two free online programs, GeoGebra and Desmos, allow students to do much more. This article will show the steps needed to insert a photograph into each program and to create a polynomial to fit the image in the photograph. We will compare the pros and cons of these programs and discuss extensions for their use in a mathematics classroom.
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Edmondson, Amanda Jane, and Simon Pini. "The pros and cons of using photographs in nursing research." Nurse Researcher 27, no. 2 (June 12, 2019): 8–13. http://dx.doi.org/10.7748/nr.2019.e1620.
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Urbahs, Aleksandrs, Sudip Das, Shravan Koundinya Vutukuru, and Kristīne Carjova. "Investigation of Flow Field Around the Pointed Cowl Air Intake at Mach 2.0." Transport and Aerospace Engineering 5, no. 1 (December 1, 2017): 75–82. http://dx.doi.org/10.1515/tae-2017-0021.
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Abstract Experiments and computational studies were carried out to get an understanding of the flow field around a rectangular supersonic intake with pointed cowl shape. Experiments include quantitative pressure measurements and flow visualization studies by using schlieren techniques. The effects of the presence of various cowl shapes on ramp surface have been obtained computationally at Mach 2.0. The experiments were carried out only for the pointed cowl. Schlieren Photographs were taken. Three-Dimensional simulations were made by using FLUENT at supersonic speed. The details of the experiments and computations are discussed.
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Zhou, T., L. Lv, J. Liu, and J. Wan. "APPLICATION OF UAV OBLIQUE PHOTOGRAPHY IN REAL SCENE 3D MODELING." International Archives of the Photogrammetry, Remote Sensing and Spatial Information Sciences XLIII-B2-2021 (June 28, 2021): 413–18. http://dx.doi.org/10.5194/isprs-archives-xliii-b2-2021-413-2021.
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Abstract. Aiming at the phenomenon that the traditional measurement methods cannot complete large-scale measurement in a short time, and the image quality obtained by remote sensing in cloudy and rainy areas is difficult to meet the demand, this paper puts forward the idea of using UAV tilt photography to build three-dimensional modeling of urban real scene. The UAV tilt photography technology is used to collect the image data of about 200 km2 in Wuzhishan City. By laying a small number of ground image control points, the aerial triangulation is used to establish the connection, and the three-dimensional modeling of the collected data is carried out. Through the field verification to verify the attribute information of ground objects, the accuracy is verified by using CORS system field coordinate collection. The results show that the ground property and mathematical accuracy of UAV tilt photography model meet the requirements, and can be effectively used in real 3D modeling.
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Potadle, Greer M., Geoff Dahl, Thaigo Fabris, Jennifer M. Bundy, and Howard Tyler. "35 Effect of Late Gestational Heat Stress on Placental Characteristics in Dairy Cattle." Journal of Animal Science 99, Supplement_1 (May 1, 2021): 34. http://dx.doi.org/10.1093/jas/skab054.059.
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Abstract To determine the effects of late gestation heat stress on placental development, dairy cows were exposed to heat stress (HT, shade) or cooled (CL, shade, fans and soakers) during the final 46 d pre-calving on the University of Florida dairy facility (temperature-humidity index; THI >68). We hypothesize heat stress (or lack of heat abatement) will reduce placental efficiency and in turn increase placental weight, surface area, and volume. At expulsion all placentae were collected and total placental weight was determined as well as individual cotyledonary weights, surface areas, and volume. In addition, the total number of total cotyledons was recorded and cotyledonary color and placental growth abnormalities (i.e. teratomas) were recorded and photographed. All data were analyzed using PROC MIXED in SAS. In addition, associations between parameters were determined by calculating Pearson Correlation Coefficients using SAS. Placentae from HT cows had a higher total placental weight, higher non-vascular membrane weight, higher total cotyledonary weight, higher total cotyledonary volume, and a higher incidence of teratomas than those from CL cows (P < 0.05). HT cows also had placentae with a significantly greater average cotyledonary weight and volume (P < 0.05). HT cows tended to have a greater incidence of color abnormalities in the placenta (P < 0.075). In addition, HT cows had significantly lighter calves at birth (P < 0.05). These data demonstrate that heat stress (or lack of heat abatement) impacts placental growth during the final stages of gestation, resulting in heavier placentae, an increase in cotyledonary weight and volume, but not an increase in the total number of cotyledons or total cotyledonary surface area. These placental alterations ultimately resulted in lighter calves at birth.
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BROSTER, WILLIAM H., and VALERIE J. BROSTER. "Body score of dairy cows." Journal of Dairy Research 65, no. 1 (February 1998): 155–73. http://dx.doi.org/10.1017/s0022029997002550.
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During the last two decades the traditional subjective appraisal of the body fat stores in farm animals, made by eye and touch, has been rationalized by the introduction of numerical systems of rating specific anatomical points.Palpation of the lumbar vertebrae, the pin and hook bones (tail head) (Lowman et al. 1973), and, occasionally, width behind the shoulders (Treacher et al. 1986) provides an assessment of the fatness of the animal. This is calibrated from standard photographic charts, in the use of which the operator is trained. Body condition scoring (CS) by this method has been widely developed for dairy cows (Earle, 1976; Mulvany, 1977; MacCarthy, 1978; Scott & Smeaton, 1980; Wildman et al. 1982; Aalseth et al. 1983; Anon. 1986; Garcia Paloma, 1990).The actual numerical scales have varied among systems. Thus over the range from very thin to very fat cows the scale is 1, 2…7, 8 (Earle, 1976) and 0½, 1,…4½, 5 (Mulvany, 1977), for example. CS is thus a discrete variate with a limited number of readings. Several authors have sought to decrease the division size, for example 1, 1 1/3; 1 2/3;…4 2/3, 5 (Ducker et al. 1985a, see also Dewhurst et al. 1996) and by using the means of the separate values obtained by two operators judging CS independently (W. H. Broster, V. J. Broster, A. J. Clements, J. W. Siviter and T. Smith, unpublished results). In this review CS will be stated in the units given by Mulvany (1977), to which other ranges have been scaled. Inspection of CS data has usually been conducted by analysis of variance, but also by the Mann–Whitney signed rank test (Moorby et al. 1996).Evans (1978) and Nicoll (1981) studied the variation in recording CS. Both investigators concluded that a system of observation by two independent assessors per recording occasion is advantageous and that revision training is necessary to maintain operator standardization. Of total variance in CS, some 60–70% was found attributable to ‘between-animal’ differences, <5% to assessor variation and <10% to animal×assessor variation. Croxton & Stollard (1976) found good repeatability of CS measurements ‘between’ and ‘within’ operators. It is regrettable that few reports of experimentation give full details of method of and number of operators engaged in body score recording.
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Zhou, Yongli. "Fulfill Your Digital Preservation Goals with a Budget Studio." Information Technology and Libraries 35, no. 1 (April 1, 2016): 26. http://dx.doi.org/10.6017/ital.v35i1.5704.
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<p>In order to fulfill digital preservation goals, many institutions use high-end scanners for in-house scanning of historical print and oversize materials. However, high-end scanners’ prices do not fit in many small institutions’ budget. As digital single-lens reflex (DSLR) camera technologies advance and camera prices drop quickly, a budget photography studio can help to achieve institutions’ preservation goals. This paper compares images delivered by a high-end overhead scanner and a consumer level DSLR camera, discusses pros and cons of using each method, demonstrates how to set up a cost efficient shooting studio, and presents a budget estimate for a studio.</p>
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Harron, Tanya. "SharePoint picture library as a searchable photo database in a small library: a program description." Journal of the Canadian Health Libraries Association / Journal de l'Association des bibliothèques de la santé du Canada 36, no. 1 (April 1, 2015): 20. http://dx.doi.org/10.5596/c15-006.
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In the digital age, librarians are being consulted on unique projects that are outside of the traditional role of responding to information requests. This paper examines the role of a solo health librarian who was tasked with locating and creating a digital photo database for her organization’s small collection of digital photographs, clipart and logos. This paper discusses the selection of SharePoint picture library as the organization’s photo repository, the steps taken by the librarian to create the database, issues with cataloguing the collection, and pros and cons of the software
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Lunghi, Enrico, Daniele Romeo, Manuela Mulargia, Roberto Cogoni, Raoul Manenti, Claudia Corti, Gentile Francesco Ficetola, and Michael Veith. "On the stability of the dorsal pattern of European cave salamanders (genus Hydromantes)." Herpetozoa 32 (October 22, 2019): 249–53. http://dx.doi.org/10.3897/herpetozoa.32.e39030.
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Photographic identification is an emerging method for recognising wild animals. This harmless methodology allows researchers to identify “naturally marked” individuals and therefore study their specific ecology and behaviour. However, before incurring potential data loss, it is recommended to test the methodology on the target species and evaluate the pros and cons. We assessed the reliability of photographic identification in adult Hydromantes salamanders from three species. Specifically, we assessed whether the dorsal pattern of adult salamanders changed over time, thus evaluating its potential use as a reliable marking methodology. We used capture-mark-recapture and controlled conditions (i.e. individuals kept in fauna boxes) to evaluate potential changes in the dorsal pattern of Hydromantes through time. We did not observe any change in the dorsal pattern in the three species during the study period. Photographic identification might be a useful marking technique for these endangered species. However, these animals are usually found in environments generally lacking light and thus, researchers must be careful in setting up proper light conditions to produce suitable pictures for individual identification of Hydromantes.
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Wade, Nicholas. "Eye-Conographs." Art & Perception 2, no. 1-2 (2014): 3–10. http://dx.doi.org/10.1163/22134913-00002023.
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Icons are pictorial images that are eye-cons: they provide distillations of objects or ideas into simple pictorial shapes. They create the impression of representing that what cannot be presented. Iconography can refer to representations of people, and it has been applied to visual artists and scientists: their portraits are often reproduced in histories of art and science. Until the nineteenth century, artists were mostly represented in pigment (paintings) and scientists on paper (engravings). After the birth of photography, both have been captured by the camera and more recently manipulated by computer. Eye-conographs are ‘perceptual portraits’ of artists and scientists; they combine facial features with the styles and phenomena with which the artists and scientists are associated.
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Chagas, Ana Carolina de Souza, Márcia Cristina de Sena Oliveira, Rodrigo Giglioti, Fernando Henrique Calura, Jenifer Ferrenzini, Moacir Rossi Forim, and Antonio Thadeu Medeiros de Barros. "Efficacy evaluation of a commercial neem cake for control of Haematobia irritans on Nelore cattle." Revista Brasileira de Parasitologia Veterinária 19, no. 4 (December 2010): 217–21. http://dx.doi.org/10.1590/s1984-29612010000400005.
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Much attention has been given to the development of botanical insecticides to provide effective natural control of cattle ectoparasites without harming animals, consumers, and environment. This study evaluated the efficacy of a commercial neem cake in controlling Haematobia irritans infestation on cattle. The study was conducted at the Embrapa Southeast Cattle Research Center (CPPSE), in São Carlos, SP, Brazil, from April to July 2008. The neem cake mixed in mineral salt in a 2% concentration was provided to 20 Nelore cows during nine weeks and had its efficacy evaluated by comparison of the infestation level against a control group. Fly infestations were recorded weekly by digital photographs of each animal from both groups and the number of flies was later counted in a computer-assisted image analyzer. Quantification of neem cake components by high-performance liquid chromatography revealed the presence of azadirachtin (421 mg.kg-1) and 3-tigloyl-azadirachtol (151 mg.kg-1) in the tested neem cake. Addition of the 2% neem cake reduced mineral salt intake in about 22%. The 2% neem cake treatment failed to reduce horn fly infestations on cattle during the 9-week study period.
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Barcelo-Fimbres, M., and G. E. Seidel. "109 EFFECTS OF LIPOLYTIC AGENTS FORSKOLIN, EPINEPHRINE AND CAFFEINE ON EMBRYONIC DEVELOPMENT AND LIPID CONTENT OF BOVINE EMBRYOS PRODUCED IN VITRO." Reproduction, Fertility and Development 21, no. 1 (2009): 154. http://dx.doi.org/10.1071/rdv21n1ab109.
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The objective of this experiment was to evaluate lipid accumulation and embryonic development of bovine morulae treated with different chemicals. A total of 2619 slaughterhouse oocytes from heifers and mature cows were matured in CDM medium (similar to SOF) plus 0.5% fatty acid-free BSA and hormones (M-CDM) for 23 h at 38.5°C in 5% CO2 in air. Frozen–thawed sperm were centrifuged through a Percoll gradient and co-cultured with matured oocytes for 18 h in F-CDM (CDM+heparin). Zygotes were cultured at 38.5°C in 5% CO2/5% O2/90% N2 in CDM-1 with nonessential amino acids, 10 μm EDTA, 0.5% fatty acid free BSA, and 0.5 mm fructose. After 60 h, resulting 8-cell embryos were cultured 120 h in CDM-2 (CDM-1+essential amino acids and 2 mm fructose). A factorial design was used with 7 treatments, 2 ovary sources (cows v. heifers), and 3 bulls (A, B and C) replicated twice for each bull (6 replicates). At Day 2.5 embryo cleavage and 8-cell rates were evaluated, and on Day 6 a total of 755 morulae were randomly assigned to the 7 treatments (control, 2 and 8 mm caffeine, 1 and 4 μm epinephrine, and 10 and 40 μm forskolin). To quantify lipid accumulation, Day 7 blastocysts were fixed and stained with 1 μg mL–1 Nile red dye, after which a digital photograph of the equatorial part of the embryo (including the inner cell mass) was taken at 200×, and fluorescence intensity was measured with Image Pro software from 0 to 255 shades for each pixel (0 = no lipids; 255 = greatest lipid accumulation), as previously reported (Biol. Reprod. 2007 (Suppl. 1), 87–88). Data were analyzed by ANOVA. No differences in cleavage rates (75 v. 68 ± 3.6%) or eight cell rates (61 ± v. 57 ± 2.8%) were found for heifer v. cow oocytes (P > 0.1); however, blastocyst rates per oocyte and per 8-cell embryo were greater for cows than heifers (20 v. 10 ± 2.1%, and 68 v. 35 ± 3.8%, respectively; P < 0.05). Treatments: 2 and 8 mm caffeine produced fewer blastocysts per morula than 1 and 4 μm epinephrine, 10 and 40 μm forskolin and the control (39, 5 v. 54, 49, 48, 54 and 52 ± 5.8%; respectively) (P < 0.01). More lipid content was found in whole embryos and trophoblast of heifer-derived than cow blastocysts (P < 0.05), and forskolin resulted in less lipid content than control, caffeine- and epinephrine-treated morulae in whole embryos, embryonic mass and trophoblasts (P < 0.05; Table 1). In conclusion, mature cows were a better source of oocytes than feedlot heifers for embryonic development. High doses of caffeine were detrimental to embryos, and the addition of the lypolitic agent forskolin reduced lipid content relative to control, caffeine and epinephrine-treated embryos. Table 1.Main effect treatment means of lipid content (arbitrary fluorescence units)
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Gard, J. A., J. Roberts, T. Braden, M. Mansour, J. Yelich, K. Irsik, O. Rae, and J. G. Wenzel. "119 ASSESSMENT OF OVARIAN FOLLICULAR DYSPLASIA UTILIZING ULTRASOUND AND HISTOLOGIC EXAMINATION." Reproduction, Fertility and Development 29, no. 1 (2017): 168. http://dx.doi.org/10.1071/rdv29n1ab119.
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A slaughterhouse study commissioned by Florida Cattleman’s Association in 2007 identified ovarian follicular dysplasia (OFD) as a primary cause of infertility in Florida beef cows. Ovaries with OFD have progressive bilateral development of solid clustered follicles containing multiple Call-Exner bodies that originate in the rete ovarii and the hilar region, and progress into the cortex to eventually form bilateral Sertoli-type granulosa theca cell tumours (GTCT). The objectives of this study were to assess the distribution of OFD in cull animals and to evaluate utilisation of ultrasound for diagnosis of OFD in cattle. Ultrasound images of the right and left ovaries from 390 cull cows and heifers representing 4 Florida ranches were made with 5-MHz linear probes (Aloka, Ibex). Then, 10 to 12 females per ranch were followed to slaughter the proceeding day for collection of reproductive tracts. The fixed ovaries were measured, sectioned para-sagittally through the hilus, photographed, and arranged in histology cassettes for complete examination of the cut surface. Large ovarian structures including corpus luteum, Graafian follicles, atretic follicles, dysplastic follicles, rete ovarii, dysplastic follicles, and tumours were counted and measured for each ovary. Ovaries with OFD were graded I to IV. Grade I OFD contained small individual dysplastic follicles with diameter less than 200 µm mostly limited to the rete ovarii and medulla. Grade II OFD possessed dysplastic follicles greater than 200 µm diameter that were present in the medulla and cortex. Grade III OFD had extensive multi-sized dysplastic follicles scattered throughout the entire cortex of the ovary and Grade IV OFD had Sertoli-type GTCT. Grade II–IV often had dystrophic mineralization of dysplastic follicles. Gross morphology of fixed sagittal sections and ultrasound images were blindly compared against OFD grade in 40 individual ovaries. The OFD was identified at slaughter in 29/41 cows and in 1/5 of heifers. The distribution of OFD for 30 affected females was Gr I 16/30, Gr II 9/30, Gr III 4/30, and Gr IV 1/30. Characteristics that could be detected by routine ultrasound included increased size and length, increased hyperechogenicity and decreased number of fluid-filled follicles. Hyperechogenic shadows were evident in higher grade OFD. The study demonstrated that Grade III and IV OFD can be observed by routine ultrasound but Grade I and II may require higher resolution ultrasound probes, imaging analysis software, or Doppler ultrasound.
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Lorenzo, Gabriela, Luciano Lopez, Reinaldo A. Moralejo, and Luis M. Del Papa. "Fotogrametría SFM aplicada a la determinación taxonómica de restos arqueofaunísticos." Virtual Archaeology Review 10, no. 20 (January 28, 2019): 70. http://dx.doi.org/10.4995/var.2019.11094.
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<p>Photogrammetry has recently been incorporated into archaeological research, replacing much more expensive techniques while still generating high resolution results. This technique converts two dimensional (2D) images into three-dimensional (3D) models, allowing for the complex analysis of geometric and spatial information. It has become one of the most used methods for the 3D recording of cultural heritage objects. Among its possible archaeological uses are: digitally documenting an archaeological dig at low cost, aiding the decision-making process (Dellepiane et al., 2013); spatial surveying of archaeological sites; 3D model generation of archaeological objects and digitisation of archaeological collections (Adami et al., 2018; Aparicio Resco et al., 2014; Cots et al., 2018; Iturbe et al., 2018; Moyano, 2017).</p><p>The objective of this paper is to show the applicability of 3D models based on SfM (Structure from Motion) photogrammetry for archaeofauna analyses. We created 3D models of four camelid (Lama glama) bone elements (skull, radius-ulna, metatarsus and proximal phalange), aiming to demonstrate the advantages of 3D models over 2D osteological guides, which are usually used to perform anatomical and systematic determination of specimens.</p><p>Photographs were taken with a 16 Megapixel Nikon D5100 DSLR camera mounted on a tripod, with the distance to the object ranging between 1 and 3 m and using a 50mm fixed lens. Each bone element was placed on a 1 m tall stool, with a green, high contrast background. Photographs were shot at regular intervals of 10-15º, moving in a circle. Sets of around 30 pictures were taken from three circumferences at vertical angles of 0º, 45º and 60º. In addition, some detailed and overhead shots were taken from the dorsal and ventral sides of each bone element. Each set of dorsal and ventral photos was imported to Agisoft Photoscan Professional. A workflow (Fig. 4) of alignment, tie point matching, high resolution 3D dense point cloud construction, and creation of a triangular mesh covered with a photographic texture was performed. Finally the dorsal and ventral models were aligned and merged and the 3D model was accurately scaled. In order to determine accuracy of the models, linear measurements were performed and compared to a digital gauge measurement of the physical bones, obtaining a difference of less than 0.5 mm.</p><p>Furthermore, five archaeological specimens were selected to compare our 3D models with the most commonly used 2D camelid atlas (Pacheco Torres et al., 1986; Sierpe, 2015). In the particular case of archaeofaunal analyses, where anatomical and systematic determination of the specimens is the key, digital photogrammetry has proven to be more effective than traditional 2D documentation methods. This is due to the fact that 2D osteological guides based on drawings or pictures lack the necessary viewing angles to perform an adequate and complete diagnosis of the specimens. Using new technology can deliver better results, producing more comprehensive information of the bone element, with great detail and geometrical precision and not limited to pictures or drawings at particular angles. In this paper we can see how 3D modelling with SfM-MVS (Structure from Motion-Multi View Stereo) allows the observation of an element from multiple angles. The possibility of zooming and rotating the models (Figs. 6g, 6h, 7d, 8c) improves the determination of the archaeological specimens.</p><p>Information on how the 3D model was produced is essential. A metadata file must include data on each bone element (anatomical and taxonomic) plus information on photographic quantity and quality. This file must also contain the software used to produce the model and the parameters and resolution of each step of the workflow (number of 3D points, mesh vertices, texture resolution and quantification of the error of the model). In short, 3D models are excellent tools for osteological guides.</p>
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Shields, C. L. "I-125 Brachytherapy for Choroidal Melanoma: Photographic and Angiographic Abnormalities: The Collaborative Ocular Melanoma Study: COMS Report No. 30." Yearbook of Ophthalmology 2010 (January 2010): 213–14. http://dx.doi.org/10.1016/s0084-392x(10)79225-x.
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Rossi-Wongtschowski, Carmen Lucia Del Bianco, Carolina Correia Siliprandi, Marina Rito Brenha, Silvia de Almeida Gonsales, Cesar Santificetur, and André Martins Vaz-dos-Santos. "Atlas of marine bony fish otoliths (Sagittae) of Southeastern - Southern Brazil Part I: Gadiformes (Macrouridae, Moridae, Bregmacerotidae, Phycidae and Merlucciidae); Part II: Perciformes (Carangidae, Sciaenidae, Scombridae and Serranidae)." Brazilian Journal of Oceanography 62, spe1 (2014): 1–103. http://dx.doi.org/10.1590/s1679-875920140637062sp1.
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The drawings, detailed pictures, precise descriptions and measurements that characterize otoliths must be made available for studies in various areas, including taxonomy, phylogeny, ecology, fisheries, paleontology, diversity, predator-prey relationships and modeling. The Collection of Teleostei Fish Otoliths of Southeastern-Southern Brazil (COSS-Brasil) of IOUSP contains 45,000 pairs of otoliths from 210 species. This publication is the first in a series that will constitute an atlas of Teleostei otoliths for southeastern-southern Brazil and presents the results of the morphologic and morphometric analyses of 11 Gadiformes and 36 Perciformes species by means of the most commonly used features, measurements and indices. Three otoliths of each species were illustrated and photographed whenever possible. The frequency of occurrence was calculated for each characteristic by total length classes (TL), and the ontogenetic differences were analyzed (multiple χ2 test; significance 0.05). Morphometric analyses were conducted for each characteristic per total length (TL) class and for the whole sample, and the ontogenetic differences were analyzed.
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Imai, K., T. Somfai, Y. Inaba, Y. Aikawa, M. Ohtake, S. Kobayashi, and K. Konishi. "208 TIME LAPSE CINEMATOGRAPHIC ANALYSIS OF CLEAVAGE AND BLASTULATION IN BOVINE EMBRYOS OBTAINED BY OVUM PICKUP AND IN VITRO FERTILIZATION." Reproduction, Fertility and Development 21, no. 1 (2009): 202. http://dx.doi.org/10.1071/rdv21n1ab208.
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Since the 1980s, several different bovine in vitro embryo production systems have been developed, and more than 291 000 embryos have been transferred throughout the world (Thibier M 2007 IETS Newsletter 25(4), 15–20). However, we have limited knowledge about the cleavage pattern of the first, second, and third cell divisions and the developmental activities of embryos during in vitro culture (IVC). The present study was conducted to determine the developmental activities of bovine embryos obtained by ovum pickup (OPU), in vitro maturation (IVM), and in vitro fertilization (IVF). We analyzed embryonic development by time-lapse cinematography (TLC). A total of 92 cumulus–oocyte complexes were collected by OPU from Japanese Black cows and were subjected to IVM and IVF as reported previously (Imai et al. 2006 J. Reprod. Dev. 52(Suppl.), S19–S29). Inseminated oocytes were cultured in microdrops of CR1aa medium supplemented with 5% calf serum covered by mineral oil in 5% CO2 in air at 38.5°C. Kinetics of embryo development were measured by TLC for 168 h after IVF by using a Cultured Cell Monitoring System (CCM–M1.4ZS, Astec, Fukuoka, Japan). A total of 672 photographs of the embryos were taken (1 photograph every 15 min) during IVC. Image stacks were analyzed by the CCM–M1.4 software. Timing of the first, second, and third cell divisions, blastulation, and embryonic contractions were recorded. The results are reported as time (h) passed after insemination. In total, 75 (81.5%) embryos cleaved and 61 (66.3%) embryos developed to the blastocyst stage. The first, second, and third cell divisions in these viable embryos occurred at 24.0 ± 0.5, 32.1 ± 0.2, and 39.4 ± 0.4 h (mean ± SE) after IVF, respectively. On the other hand, in nonviable embryos (those that failed to develop to the blastocyst stage; n = 14), these cell divisions occurred at 29.5 ± 2.2, 41.3 ± 3.3, and 57.2 ± 7.6 h after IVF, respectively. There tended to be a difference (P = 0.06; paired t-test) in the timing of the first cell division between viable and nonviable embryos. Blastulation of embryos began at 114.4 ± 1.1 h, embryos developed to the blastocyst stage at 127.3 ± 1.4 h, and blastocysts began to expand at 138.4 ± 1.7 h after IVF, respectively. During blastocyst development, embryonic contractions (shrinkage attributable to the rupture of the blastocoele) and tight-shrinkage (shrinking of the embryo to less than 70% of its surface area) were observed in all embryos. The mean numbers of contractions and tight-shrinkages in blastocysts were 5.3 ± 2.7 and 2.1 ± 1.0 times, respectively. The frequency of contractions from the beginning of blastulation to the blastocyst stage was significantly lower (P < 0.01) than after the blastocyst stage. It took 6.9 ± 4.6 h for the embryos to re-expand after the tight-shrinkages. These results indicate that viable in vitro-produced embryos can be selected at early stages by TLC. Further studies are necessary to clarify the importance of the pulsating activity in OPU–IVF embryos. This work was supported by the Research and Development Program for New Bio-industry Initiatives.
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Imai, K., S. Sugimura, T. Somfai, Y. Inaba, Y. Aikawa, M. Ohtake, M. Hirayama, et al. "159 RELATIONSHIP BETWEEN CLEAVAGE PATTERNS OF FIRST CELL CYCLE AND POST-TRANSFER VIABILITY IN BOVINE EMBRYOS OBTAINED BY OVUM PICKUP AND IN VITRO FERTILIZATION." Reproduction, Fertility and Development 24, no. 1 (2012): 191. http://dx.doi.org/10.1071/rdv24n1ab159.
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More than 300 000 embryos have been transferred all over the world (Stroud 2010 IETS Newsl. 27(4), 11–21). We have reported that embryos that showed the abnormal cleavage pattern at the first cell division can develop to the blastocyst stage (Somfai et al. 2010 J. Reprod. Dev. 56, 200–207). However, we have limited knowledge about the consequences of the pattern of first embryonic cleavage on their post-transfer developmental competence. The present study was conducted to determine the developmental competence of bovine blastocysts showing different cleavage patterns at their first cell division. Cumulus–oocyte complexes were collected by ovum pickup from Japanese Black cows and were subjected to in vitro maturation and IVF as reported previously (Imai et al. 2006 J. Reprod. Dev. 52, S19–S29 suppl). Inseminated oocytes were cultured in CR1aa medium supplemented with 5% calf serum covered by mineral oil at 38.5°C in 5% CO2 in air with micro-droplets or 5% CO2, 5% O2 and 90% N2. The kinetics of embryo development were analysed by time-lapse cinematography for 168 h after IVF by using a Cultured Cell Monitoring System (CCM-M1.4ZS, Astec, Fukuoka, Japan). A total of 673 photographs of each embryo were taken (1 photograph in every 15 min) during in vitro culture. Image stacks were analysed by the CCM-M1.4 software. Embryos were classified in 5 groups according to the pattern of first cleavage as normal cleavage (NC), direct cleavage from 1 cell to 3 to 4 blastomeres (3–4BL), unequal blastomeres (UB), multiple fragments (MF) and protrusion formation (PT). Blastocysts developing from each group were transferred into the ipsilateral uterine horn of each synchronized recipient on Day 7 or 8 after oestrus. Data on conception at Day 60, abortion and delivery were then recorded. Data were analysed by chi-square test and Student's t-test. In total, 43 embryos were transferred, 17 conceptions (39.5%) were established and 16 recipients (94.1%) were delivered. Only 1 abortion was detected at Day 223 in the NC group. The highest conception rate was observed in the NC group (55%, n = 20) and the 3–4BL (n = 12), UB (n = 6) and PT (n = 3) groups showed similar conception rates of 33.3% (1 implanted embryo belonged to 2 classes in UB and PT) and none of the embryos derived from the MF group (n = 3) could cause conception. There was a significant difference (P < 0.05) in conception rates between the NC group and totals of each of the other cleavage groups. No significant difference was found in gestation lengths and birth weights between the NC group (282.2 ± 4.4 days, 30.6 ± 3.8 kg, respectively) and totals of each of the other cleavage groups (282.8 ± 5.3 days, 30.3 ± 1.9 kg, respectively). These results indicate that embryos showing abnormal cleavage patterns at first cell division can develop to normal calves with normal gestation lengths and birth weights; however, their post-transfer viability is lower than for NC embryos. This work was supported by the Research and Development Program for New Bio-industry Initiatives.
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Graham, Andrew, Stephan Gmur, and Travis Scott. "Improved SCAT data workflow to increase efficiency and data accuracy." International Oil Spill Conference Proceedings 2017, no. 1 (May 1, 2017): 2674–93. http://dx.doi.org/10.7901/2169-3358-2017.1.2674.
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ABSTRACT #2017-302 Traditional Shoreline Cleanup Assessment Technique (SCAT) data workflows typically entail collecting data in the field using notebooks, handheld GPS units and digital cameras, transcribing these data onto paper forms, and then manually entering into a local database. Processed data are pushed to a SCAT geographical information system (GIS) specialist, ultimately providing exports as paper and electronic versions of maps, spreadsheets and reports. The multiple and sometimes iterative steps required can affect the dissemination of accurate and timely information to decision makers and compound the potential for introducing errors into the data. To improve this process a revised SCAT data workflow has been developed that decreases data processing steps and time requirements while increasing data accuracy in several facets of the process. The workflow involves using mobile data collection devices in the field to capture attribute data, photographs and geospatial data. These data are uploaded to a web-enabled database that allows field team members to complete, review and adjust their data, along with data manager approval before presentation to others in the response. For response personnel with internet access and proper login credentials, SCAT data, including photographs, reports and results can be searched for by attribute, time or location, and reviewed online in form view or on a web map. For traditional SCAT spatial analysis products, approved data can be exported and processed in a GIS as normal, but can also be returned to the web-enabled database to be viewed on a map or distributed via web mapping services (WMS) to other web GIS data viewers or common operating pictures (COPs). Field testing of the improved workflow shows decreased data processing time for data, a more robust yet streamlined quality assurance and quality control process (QA/QC), and easier more inclusive access to the data relative to traditional paper forms and data processing. While the improved workflow entails a steeper learning curve and a heavier reliance on technology than traditional SCAT workflows, the benefits are significant.
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Sadikova, Sitora-bonu, and Sabine Hennig. "National nature-based tourism in Samarkand Region." InterCarto. InterGIS 26, no. 3 (2020): 423–34. http://dx.doi.org/10.35595/2414-9179-2020-3-26-423-434.
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The article analyses the data on the assessment of the potential of nature-based tourism in Samarkand Region. Esri’s ArcGIS social Survey 123 Onlinewas created for data collection and subsequent analysis in statistical programs. This method revealed vacation spots Amankutan, Agalyk, Mirankul, Tepa-kul, Nurbulok, Dobusiya, Yukori Chinor, Ming Archa, Kattakurgan Reservoir, Zarafshan river and others on the basis of opinions of representatives of local population of Uzbekistan about each location. The sociological survey identified: the most polluted vacation spots; the pros and cons of specific suburban recreation areas; popular types of active and passive suburban recreation; opinion on effective penalties for vandalism and damage to flora and fauna. Eco tourism and nature tourism should be clearly distinguished. Natural tourism is all types of tourism that use natural resources in wild or undeveloped natural areas: bird watching, forest trips, safaris, fishing, hunting, photography. It should be noted that ecotourism is distinguished by satisfying the recreational needs of visitors and hosts, for the benefit of the local population and without negative impact on the ecology of the region. “Eco” is obliged to serve for the benefit of nature, its biodiversity and ecology. Consequently, the development of ecotourism contributes to the improvement of the ecological, educational and cultural level of the state society as a whole. The recommendations for effective environment-friendly use of nature-based tourism potential of Samarkand Region of Uzbekistan are presented.
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Marchenko, N. I. "FORMING ECONOMICALLY USEFUL TRAITS OF MEAT PRODUCTIVITY, INTRAMUSCULAR FATTING OF BULL-CALVES OF DIFFERENT GENOTYPES AND AT DIFFERENT AGE PERIOD." Animal Breeding and Genetics 51 (March 28, 2018): 106–11. http://dx.doi.org/10.31073/abg.51.14.
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Introduction. In 1997, Valeriy P. Burkat proposed by one of the methodological and organizational approaches to creating Simmental Beef cattle, to launch a series of experiments to study effectiveness of Simmental crossing with other breeds and to study the best combination for interbreed industrial crossing. The studies of comparative determination of meat quality at crossing of Simmental with specialized beef breeds found that these hybrids were well fattening and they had highly meat productivity.
The methods of studying fattening and meat qualities of cattle recommend to conduct slaughtering and processing animals at meat processing plants, because under these conditions the standards for assessing meat productivity of animals and quality of their carcasses can be followed exactly. Compliance with instructions, recommendation for processing animal carcasses under such conditions and obtaining research results will be comparable with the slaughter of animals of different breeds, because all technological methods and sampling are conducted by specialists.
General rules for testing laboratories for determination of quality parameters of agricultural raw materials and products under market conditions require the following: availability of specialists, a plan of sampling and the fastest transportation of them to place of laboratory testing, determination of research methods and conducting specific research, identified according to program (task) with followed analysis.
Materials and methods. Experimental samples were the longest back muscle sampled from refrigerated right half-carcasses of bull-calves at the age of 12 months and 16 months. The animals were obtained from Black-and-White cows (BW) and bulls of Ukrainian Beef (UB), Volyn Beef (VB), Polesian Beef (PB), Simmental (S) breeds, grown in "Polesia", Ovruch district, Zhytomyr region. The control slaughter of experimental animals, followed deboned half-carcasses, was conducted by experts of Ovruch slaughterhouse, Zhytomyr region. The samples of the longest back muscle were taken at 9-12 ribs of refrigerated half-carcasses of clinically healthy bull-calves. The samples of muscle were labelled and fixed in a 10% solution of neutral formalin, cross sections were obtained using freezing microtome, histological specimens were produced by the method of colouring the muscle fat, performing a series of research works to enhance and improve existing methods for obtaining excellent results.
Microscopy, morphometry and microscopic photography of histological sections of the longest back muscle were carried out using biological research microscope (MBI-6), division of muscular fibers by their size was conducted via ocular grid. Analysis of research results was statistically processed on a PC.
Results. Based on the results of the experiment the higher live weight before slaughter was at bull-calves obtained from crossing with Simmental and Polesian Beef bulls, 304 kg and 285 kg, respectively in these groups, which was 53 kg and 72 kg more compared with animals of the same age of Black-and-White dairy breed. It is obvious that the formation of meat productivity from early age is associated with belonging to breed, specific soil and climatic and economic conditions at that time. All the cross-breeds of the research groups had better live weight before slaughter and weight of pair and chilled carcasses. But the greatest bone weight had animals of the same age of two research groups – ½PB½BW and ½UB½BW, respectively 16,8 kg and 15,8 kg. It is because of blood of Charolais bulls and consolidated trait of angularity during the selection.
The highest live weight before slaughter had half-blooded genotypes with Simmental and Polesian Beef – 354 and 343 kg; it was slightly below compared with the target breed standard. The largest weight of pair carcasses had genotypes ½S½BW and ½PM½BW – 194 ± 6,2 and 193 ± 5,0 kg, the highest meatiness index had Volyn Beef counterparts at the level of 4.3 units.
Medium muscle fibers (21-40mkm) had the highest share, from 72.4 to 43.6%, at the investigated bull-calves at the age of 12 months. Morphological structure of muscle of bulls at 16 months of age showed that average diameter of fine fibers at the animals of meat productivity was greater – 18.1mkm in combination with Simmental, 18.3 and 18.5 mkm – with Ukrainian and Polesian Beef against 17,9 mkm – at the counterparts of Black-and-White breed. The share of fine fibers was very high (15.3%) at the bull-calves of genotype with Ukrainian Beef against 7.3% and 7.2% – with Polesian Beef and Simmental. Some fat deposition and the small number of fat cells, located mainly around blood vessels, were observed in the longest back muscle of Black-and-White dairy bull-calves at the age of 12 months. Such changes in the number of fibers and size of their diameters, to some extent, affect the quantitative (meatiness index) and qualitative (the number of intramuscular fat) productivity characteristics of the investigated animals.
Conclusions. Trend towards more intense growth and accumulation of muscle and fat was revealed at the researched bull-calves of meat productivity. Formation of economically useful traits on meat productivity of bull-calves depends on age, breed, genotypic and phenotypic factors. The results of histological studies indicate that internal structure of the longest back muscle and the ratio of different types of muscle fibers (thin, medium, thick), show breed and age features of meat productivity formation at bull-calves.
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Kolodyazhny, Sergey A., Sergei N. Zolotukhin, Anatoly A. Abramenko, and Yekaterina A. Artemova. "Destruction of buildings and use of materials from renovated urban territories." Vestnik MGSU, no. 2 (February 2020): 271–93. http://dx.doi.org/10.22227/1997-0935.2020.2.271-293.
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Introduction. Reuse of construction materials, products, and structures from demolished buildings and installations is an actual problem. The solution to it will improve the ecological situation. The study is aims at the determination of the causes of urban area renovation and searches for the most efficient technologies of building demolition with the reuse of the generated materials, products, and structures.
Materials and methods. The study used systematization, structural analysis, comparative analysis, a theoretical generalization of the data obtained in a detailed analysis of literary and statistical sources, field surveys of objects. When field examining, photography methods were used.
Results. Existing technologies of the building demolition, construction waste recovery, and reuse of construction materials generated using innovative item-by-item demolition technologies were revealed and systematized. Causes and main trends of urban territory renovation were analyzed. The paper studied Russian and foreign technologies of the building and installation demolition. The pros and cons of various demolition types are shown in terms of their environmental friendliness and economic efficiency.
Conclusions. The experience of constructing low-rise buildings and intrasettlement roads showed that the item-by-item disassembly of buildings using modern equipment and machinery with the subsequent reuse of construction materials, products, and structures is efficient, economical, and environment-friendly. It is proven that the reuse of construction materials, products, and structures results in a significant reduction in the cost of erected installations. The results of the study can be applied in low-rise construction around the world.
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Guigné, Anna Kearney. "Cows Don’t Know It’s Sunday: Agricultural Life in St. John’s. By Hilda Chaulk Murray. (St. John’s: Institute of Social and Economic Research, 2002. Pp. 338, photographs, index, bibliography ISBN 0-919666-53-1)." Ethnologies 29, no. 1-2 (2007): 383. http://dx.doi.org/10.7202/018768ar.
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Matoba, S., S. Sugimura, H. Matsuda, Y. Aikawa, M. Ohtake, S. Kobayashi, E. Horiguchi, Y. Hashiyada, M. Nagai, and K. Imai. "236 KINETICS AND PATTERN OF THE FIRST CLEAVAGE OF IN VITRO-FERTILIZED EMBRYOS BY IN VIVO-MATURED OOCYTES AND X-SORTED SPERMATOZOA IN DAIRY CATTLE." Reproduction, Fertility and Development 25, no. 1 (2013): 266. http://dx.doi.org/10.1071/rdv25n1ab236.
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Recently, we reported that high rates of good-quality blastocysts can be produced by IVF of in vivo-matured oocytes, obtained by ovum pick-up (OPU) after superstimulation in Holstein cows, with X-sorted sperm [Matoba et al. 2012 Reprod. Domest. Anim. 47(Suppl. 4), 515]. However, we have limited knowledge concerning the normality of embryonic cleavages in such embryos. The present study examined their kinetics and pattern of the first cell cycle. In vivo-matured oocytes were collected by OPU from non-lactating Holstein cows just before ovulation after superstimulation and ovulation induction by gonadotropin-releasing hormone. The oocytes were inseminated with 5 × 106 sperm mL–1 of X-sorted sperm and cultured in CR1aa supplemented with 5% newborn calf serum and 0.25 mg mL–1 of linoleic acid albumin at 38.5°C in 5% CO2, 5% O2, and 90% N2 for 216 h. Embryo kinetics were observed individually using a microwell culture dish and time-lapse cinematography (CCM-1.4MZS, Astec, Fukuoka, Japan) (Sugimura et al. 2010 Biol. Reprod. 83, 970–978). Photographs of each embryo were taken every 15 min during the in vitro culture period, and images were analysed by CCM-1.4 software (Astec). The cleavage pattern was categorised into normal cleavage (2 even blastomeres without fragment or protrusion) or abnormal cleavage (those with 2 uneven blastomeres, with fragments or protrusions and those dividing into 3 to 5 blastomeres at the first cleavage). Data were analysed by ANOVA, chi-square, and discriminant function. A total of 117 embryos were examined; of this number, 63.2% developed to the blastocyst stage and the rest were degenerated. A high rate of normal cleavage and a low rate of abnormal cleavage, including those with 2 uneven blastomeres and those with fragments or protrusions in the first cleavage pattern, were recorded in embryos that could develop to blastocysts compared with degenerated ones (P < 0.01 or P < 0.05, respectively; Table 1). No significant difference was found in those dividing into 3 to 5 blastomeres between the blastocysts and degenerated embryos (Table 1). Embryos developing to the blastocyst stage had a shorter duration of the first cell cycle [27.2 ± 2.3 h post-insemination (hpi)] compared with those undergoing degeneration (30.6 ± 5.7 hpi; P < 0.001). The threshold of duration of the first cell cycle was calculated by (X – 27.2)/2.3 = (30.6 – X)/5.7, resulting in X = 28.2. Blastocysts with a short duration of the first cell cleavage (≤28.2 hpi) showed a higher frequency of the normal cleavage pattern than those with a duration of the first cell cleavage longer than 28.2 hpi (71.7 and 53.6%, respectively; P < 0.05). Our results revealed that those IVF embryos that finished their first cleavage before 28.2 h of IVF and showed a normal cleavage pattern had superior developmental competence. Table 1.The first cleavege pattern reflects the developmental competence: blastocysts versus degenerated embryos This work was supported by the Research and Development Projects for Application in Promoting New Policy of Agriculture, Forestry and Fisheries (22016).
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Kaul, Jyoti, Ramesh Kumar, Usha Nara, Khushbu Jain, Dhirender Olakh, Tanu Tiwari, Om Prakash Yadav, and Sain Dass. "Development of Database of Maize Hybrids and Open Pollinated Varieties Released and Notified for Cultivation in India." Journal of Agricultural Science 9, no. 10 (September 13, 2017): 105. http://dx.doi.org/10.5539/jas.v9n10p105.
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The maize database, first of its kind in India, is a central repository for cultivars i.e. hybrids and open pollinated varieties (OPVs) notified for cultivation in India since the inception of All India Coordinated Maize Improvement Project (AICMIP) in 1957. The database includes the information on cultivars developed from public as well as private breeding programmes. Besides, information on registered germplasm is also given. The database carries image gallery showcasing photographs of cobs/standing crop of the public-bred cultivars released after 1993.The database also presents information about adaptability of cultivars, average yield and disease, and insect-pest resistance along with the parental materials used in breeding programmes. Information on 31 descriptors as per Distinctivity, Uniformity and Stability (DUS) tests in respect of parental lines and their hybrids that were filed for protection under “Protection of Plant Varieties and Farmers Rights Act, 2001” (PPVFRAct, 2001) has been supplemented. In addition, the database provides contact information on developers of the notified cultivars thereby facilitating interactions among the members of maize community. The information contained within maize database can be accessed at on-line expert system called maize AGRIdaksh (www.iimr.res.in/maizeexpertsystem/www.agridaksh.iasri.res.in/maize). Information on notified cultivars (1961-2010) parental lines and cultivars (1993-2012) filed under PPVFRAct, 2001 can also be accessed at www.iimr.res.in/maizeexpertsystem/maize hybrids and composite varieties released in India. Whereas, information about registered germplasm (2003-2012) can be accessed at www.iimr.res.in/publications.
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Webb, Carmen, Vishal Sharma, and Claire Temple-Oberle. "Delivering Breast Reconstruction Information to Patients: Women Report on Preferred Information Delivery Styles and Options." Plastic Surgery 26, no. 1 (January 9, 2018): 26–32. http://dx.doi.org/10.1177/2292550317750139.
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Purpose: To discover missed opportunities for providing information to women undergoing breast reconstruction in an effort to decrease regret and improve patient education, teaching modalities, and satisfaction. Method: Thirty- to 45-minute semi-structured interviews were conducted exploring patient experiences with information provision on breast reconstruction. Purposeful sampling was used to include women with a variety of reconstruction types at different time points along their recovery. Using grounded theory methodology, 2 independent reviewers analyzed the transcripts and generated thematic codes based on patient responses. BREAST-Q scores were also collected to compare satisfaction scores with qualitative responses. Results: Patients were interested in a wide variety of topics related to breast reconstruction including the pros and cons of different options, nipple-sparing mastectomies, immediate breast reconstruction, oncological safety/monitoring and the impact of chemotherapy and radiotherapy, secondary procedures (balancing, nipple reconstruction), post-operative recovery, and long-term expectations. Patients valued accessing information from multiple sources, seeing numerous photographs, being guided to reliable information online, and having access to a frequently asked questions file or document. Information delivery via interaction with medical personnel and previously reconstructed patients was most appreciated. Compared with BREAST-Q scores for satisfaction with the plastic surgeon (mean: 95.7, range: 60-100), informational satisfaction scores were lower at 74.7 (50-100), confirming the informational gaps expressed by interviewees. Conclusions: Women having recently undergone breast reconstruction reported key deficiencies in information provided prior to surgery and identified preferred information delivery options. Addressing women’s educational needs is important to achieve appropriate expectations and improve satisfaction.
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Anderson, David G. "Cows, Kin, and Globalization: An Ethnography of Sustainability. By Susan A. Crate. Globalization and the Environment, no. 3. Lanham, Md.: Alta Mira Press, 2006. xxvii, 354 pp. Notes. Bibliography. Glossary. Index. Illustrations. Photographs. Tables. Maps. $29.95, paper." Slavic Review 67, no. 2 (2008): 531–32. http://dx.doi.org/10.1017/s0037677900024372.
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Matoba, S., S. Sugimura, H. Matsuda, Y. Aikawa, M. Ohtake, S. Kobayashi, E. Horiguchi, Y. Hashiyada, M. Nagai, and K. Imai. "138 COMPARISON OF KINETICS AND PATTERNS OF THE FIRST CLEAVAGE OF IN VIVO AND IN VITRO-MATURED HOLSTEIN OOCYTES AFTER IN VITRO FERTILIZATION WITH X-SORTED SPERM." Reproduction, Fertility and Development 26, no. 1 (2014): 182. http://dx.doi.org/10.1071/rdv26n1ab138.
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Previously, it was reported that a high rate of good quality blastocysts were produced by IVF of in vivo-matured oocytes, obtained by ovum pick up (OPU) after superstimulation in Holstein cows, using X-sorted sperm (Matoba et al. 2012 Reprod. Domest. Anim. 47, 515). In this system, an early first cleavage within 28 h after IVF was found to be a potent marker for the selection of embryos with high developmental competence (Matoba et al. 2013 Reprod. Fertil. Dev. 25, 266). However, we have limited knowledge on the timing and normality of embryonic cleavages in in vitro-matured oocytes after IVF. The purpose of the present study was to compare the kinetics and patterns of the first cleavage of in vivo- and in vitro-matured bovine oocytes after IVF with X-sorted sperm. In vivo-matured oocytes (Group A) were collected by OPU from non-lactating Holstein cows just before ovulation after superstimulation. Immature oocytes were either collected by OPU without hormonal treatment or by aspiration of ovaries at the local abattoir and matured in vitro (Group B or C). All the oocytes were inseminated with 5 × 106 sperm mL–1 of X-sorted sperm, except half of oocytes in Group C inseminated by non-sorted sperm (Group D) and cultured in CR1aa supplemented with 5% calf serum and 0.25 mg mL–1 of linoleic acid albumin at 38.5°C in 5% CO2, 5% O2, and 90% N2 for 216 h. Embryo kinetics were observed individually using a microwell culture dish and time-lapse cinematography (Sugimura et al. 2010 Biol. Reprod. 83, 970–978). Photographs of each embryo were taken in every 15 min during the IVC period and analysed by time-lapse cinematography software. Cleavage pattern was categorized as normal (2 even blastomeres without fragment or protrusion) or abnormal (2 uneven blastomeres, with fragment or protrusion and those dividing into 3–5 blastomeres) at the first cleavage. Data were analysed by ANOVA, chi-squared, or discriminant function. A total of 268 cleaved embryos were used. The blastocyst rate in Group A was higher than in Groups B and C (61.3 v. 40.0 and 25.0%, respectively; P < 0.05). The timing of first cleavage was longer in Group A compared with Groups C and D (28.3 ± 3.8 v. 27.6 ± 3.8 and 26.7 ± 1.9 h, respectively) and in Group B (28.1 ± 4.0 h) compared with in Group D (P < 0.05). Higher rates of normal cleavage were observed in Groups A, B, and D than in Group C (53.5, 44.4, and 54.8 v. 16.7%, respectively; P < 0.01). The frequency of blastocysts derived from the early (28.3 h) and normal pattern cleaving oocytes were greater in Groups A and B than in Group C (29.0 and 20.0 v. 8.3%, respectively; P < 0.05) and similar in Group D (22.6%). Our results reveal that IVF embryos produced from in vivo-matured oocytes with sex-sorted sperm had superior normality than those produced from in vitro-matured oocytes and similar normality to embryos inseminated with non-sorted sperm. Supported by the Research and Development projects for application in promoting new policy of agriculture, forestry and fisheries (22016) and by JSPS and HAS under the Japan-Hungary Research Cooperative Program.
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Harl, A. W., E. L. Larimore, A. Al Naib, L. K. Wooldridge, A. D. Ealy, G. A. Perry, and M. L. Rhoads. "182 MATURATION OF BOVINE CUMULUS-OOCYTE COMPLEXES WITH FOLLICLE FLUID VARYING IN ESTRADIOL CONTENT AFFECTS CUMULUS CELL EXPANSION WITHOUT AFFECTING SUBSEQUENT EMBRYO DEVELOPMENT IN VITRO." Reproduction, Fertility and Development 29, no. 1 (2017): 199. http://dx.doi.org/10.1071/rdv29n1ab182.
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The objective of this work was to determine how characteristics of bovine follicle fluid (FF; especially oestradiol content) affect cumulus cell expansion and oocyte competence. In the first study, FF was collected from abattoir-derived ovaries and pooled separately for large follicles (≥10 mm) and small follicles (≤3 mm). A portion of the FF from each category was charcoal stripped. These 4 types of FF were then used as the primary ingredient (75% vol/vol) in oocyte maturation media. A separate control group lacking FF but containing BSA was included to monitor potential impacts of protein on outcomes (control; without FF). Some of the cumulus-oocyte complexes (COC; n = 250) were matured in individual drops for analysis of cumulus expansion (photographed and measured at 0 and 21 h of maturation). Other COC (n = 770) were matured in groups of 12 to 25 in the previously described media, and then subjected to IVF procedures. Cleavage rates were recorded on Day 3, and blastocyst rates were recorded on Day 8 post-fertilization. Cumulus cell expansion was greatest when COC were matured in medium containing FF from large follicles, wherein it even exceeded the controls (P < 0.02). Maturation in FF from small follicles resulted in cumulus expansion that was intermediate between large and control. Maturation in charcoal-stripped FF severely restricted cumulus cell expansion (P < 0.05) compared with those matured in untreated FF. Despite the observed improvement in cumulus cell expansion, COC that had been matured in media containing FF were less likely to cleave (P < 0.05) and also less likely to develop to the blastocyst stage (P < 0.01) than those matured in control medium. Cleavage and blastocyst rates did not differ among any of the maturation media containing FF. In the second study, oestrous cycles of 9 crossbred cows were synchronized and FF samples were collected 36 to 42 h after prostaglandin F2α injection. Samples from individual cows were categorized as having high oestradiol (>800,000 pg mL−1; H) or low oestradiol concentrations (<800,000 pg mL−1; L). The FF was retained for use in in vitro experiments, where it was added to maturation media (20% vol/vol). Cumulus-oocyte complexes (n = 1,775) were randomly distributed into treatments across 12 in vitro maturation/fertilization replicates (H and L, balanced within replicate; 4 replicates/cow). Each replicate included the following 3 control groups: maturation medium containing BSA without FF, maturation medium without BSA with abattoir-derived FF, and maturation medium without BSA and without FF. The COC were matured in their assigned medium for 21 h, and then all COC were subjected to IVF procedures. Cleavage rates were recorded on Day 3, and blastocyst rates were recorded on Day 7 and 8 post-fertilization. Oestradiol content of the FF (H v. L) did not affect oocyte cleavage nor blastocyst rates on Day 7 or 8. The results of these studies indicate that although FF improves cumulus cell expansion during maturation in vitro, it does not result in higher rates of cleavage or blastocyst development regardless of oestradiol content.
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Pekkanen, Tuomo. "De Finnis cornutis." Nordlit, no. 33 (November 16, 2014): 223. http://dx.doi.org/10.7557/13.3174.
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<p><em>Horned Finns</em>. The ‘cornuti Finni’ mentioned in the <em>Historia Norwegiae</em> have not found their explanation, because the Latin word <em>cornu</em>, from which the adjective is derived, has been understood in the strict sense of ‘horn on the head’. The Latin word, however, also means ‘hoof’ of horses or ‘cloven hoof’ of cows and goats, even of the mythologic Faunus and Pan. In December 1913 Kai Donner saw in Dudinka Avam-Samojeds, who because of their cylindrically shaped reindeer winter boots, the front of which was hoof-shaped, were called ‘hoofed men’ (in Finnish ‘kaviolliset miehet’). In the extracts of Aristeas of Proconnesus, who as early as about 625 B.C travelled in North-Eastern Asia, the <em>Aigipodes</em>, men with goats feet, are mentioned as inhabitants of Northern Siberia. In the first century A.D., Pomponius Mela knows <em>Hippopodes</em>, men with horse’s feet, in the Northmost Sarmatia. The three names, <em>cornuti Finni</em>,<em> Aigipodes</em> and <em>Hippopodes</em> find their explanation in the reindeer boots, necessary in the arctic climate. Recent photographs of the Ngasani and Nenets Samojed show that this kind of boots are still used. The fact that some arctic peoples have since ancient times got their nickname from their boots, which made them look like hoofed animals, makes it necessary to reconsider the origin of the names Lapp and Finn, the etymology of which is unclear. As the Swedish <em>lapp</em>, earlier form of modern <em>labb</em>, is of Indogermanic origin and has in several languages the meaning of the foot of a quadruped animal, it seems that the name Lapp, considered as abusive by the Sami, belongs to the arctic names given to people who because of their boots looked like men with goat’s or horse’s feet and were called, as Donner heard it, ‘hoofed men’. It remains an open question whether the ethnonym <em>Fenni</em> / <em>Finni</em> may be of similar origin.</p>
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Kaiser, G. G., P. J. Ross, K. Wang, and J. B. Cibelli. "61 MITOCHONDRIA DISTRIBUTION IN FERTILIZED, PARTHENOGENETIC, AND CLONED BOVINE EMBRYOS." Reproduction, Fertility and Development 22, no. 1 (2010): 188. http://dx.doi.org/10.1071/rdv22n1ab61.
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In this study we evaluated mitochondrial distribution of individual bovine embryos after IVF, parthenogenetic activation (PG), and somatic cell nuclear transfer (SCNT). COCs obtained from slaughterhouse ovaries were matured in vitro for at least 18 h in TCM-199 supplemented with hormones, and then divided into 3 groups. SCNT and PG oocytes were stripped by vortexing in HEPES-HECM (hamster embryo culture medium) medium (HH) containing hyaluronidase and metaphase II (MII) oocytes selected by visualization of a polar body. The PG group oocytes were exposed 24h post-maturation to 5 μM ionomycin in HH for 4 min, then rinsed 3 times in HH and allocated to a 4-h culture in 2 mM DMAP in KSOM for activation. The SCNT group oocytes were included in a nuclear transfer procedure performed as previously described (Ross et al. 2006 Biotechniques 41, 741-750). Activation was performed as described for the PG group. The IVF group COCs were co-incubated for 20 h with 106 spermatozoa/mL in IVF-TALP supplemented with heparin. To label mitochondria, 1 mM MitoTracker CMXRos Red (Molecular Probes, Eugene, OR, USA) was added to HH at a final concentration of 0.3 μM. Samples were cultured for 15 min, washed in HH, placed in a glass-bottomed 35-mm Petri dish, and then observed and live photographed by using a spinning disk confocal microscope (Nikon Eclipse TE2000-E + CARV Confocal) equipped with a Cascade 512 B camera (Roper Scientific, Tucson, AZ, USA) using a Nikon 40×, 1.3 NA oil objective lens. Z series images were taken acquiring 15 focal planes at 10-μm intervals. Analysis was performed using Metamorph software. Samples were taken at pronuclear, 4 cell, and morula stages. Each sample was classified for its mitochondrial localization in pericytoplasm, cytoplasm, and perinuclear. Data was analyzed by proc glimmix (SAS, Cary, NC, USA). Significance was set at P < 0.05. A similar pericytoplasmic distribution of mitochondria for all treatments up to the 4-cell stage was observed. At the pronucelar stage, mitochondria distribution was mostly pericytoplasmic, changing to cytoplasmic at the 4-cell stage. At the morula stage there was a significantly higher number of embryos with perinuclear distribution in IVF than in PG and SCNT embryos (Table 1). Our findings demonstrate that mitochondrial reorganization differs in fertilized more-developed embryos compared with their activated counterparts. This may have implications for further embryo development, mainly after SCNT. Table 1.Mitochondria distribution in fertilized, parthenogenetic, and cloned bovine embryos
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Imai, K., M. Ohtaku, Y. Aikawa, H. Matsuda, S. Kobayashi, E. Horiguchi, S. Matoba, and Y. Hashiyada. "137 EFFECTS OF X-SORTED SPERM IN QUALITY OF BOVINE BLASTOCYST DERIVED FROM IN VIVO-MATURED OOCYTES." Reproduction, Fertility and Development 26, no. 1 (2014): 182. http://dx.doi.org/10.1071/rdv26n1ab137.
Full textAbstract:
Recently, we reported on a promising system for selecting healthy IVF embryos in cattle using kinetics of early embryo development and oxygen consumption of blastocyst [Sugimura et al. 2012 PLoS ONE 7, e36627]. The present study was conducted to examine the differences in embryo quality of bovine blastocysts obtained after IVF of in vivo-matured oocytes with X-sorted and unsorted sperm. Holstein dry cows (n = 8) were reared under the same feeding and environmental conditions. Two ovum pickup (OPU) sessions were conducted in each cow to fertilize with or without X-sorted sperm. In vivo-matured oocytes were collected by OPU just before ovulation after superstimulation treatment. The oocytes were inseminated with 5 × 106 sperm mL–1 of each sperm, and presumptive zygotes were cultured in CR1aa supplemented with 5% newborn calf serum and 0.25 mg mL–1 of linolenic acid albumin at 38.5 C in 5% CO2, 5% O2, and 90% N2 for 168 h. Embryo kinetics were observed individually using a microwell culture dish (Dai-Nippon Print) and time-lapse cinematography (CCM-1.4MZS; Astec, Fukuoka, Japan; Sugimura et al. 2010 Biol. Reprod. 83, 970–978). Photographs of each embryo were taken every 15 min during the in vitro culture period and images were analysed by CCM-1.4 software (Astec). By assessing the quality of blastocysts, a combination of identified prognostic factors were used: (1) timing of the first cleavage (less than 27 h post-insemination); (2) two blastomeres at the end of the first cleavage; (3) absence of fragments at the end of the first cleavage; and (4) six or more blastomeres at the onset of the lag-phase. Data were analysed by ANOVA. In total, 34.1 ± 18.4 oocytes per session per donor were collected by OPU, and 23.7 ± 13.4 oocytes had an expanded cumulus cell. Oocyte recovery rates were recorded at 77.1 ± 15.1%. After IVF and in vitro culture, 10.6 ± 7.7 blastocysts per session per donor were produced in this study. There was no significantly difference in cleavage rates and blastocyst formation rates between X-sorted sperm and unsorted sperm (87.1 ± 10.8 and 82.6 ± 12.1% and 38.4 ± 23.6 and 57.1 ± 23.4%, respectively). However, blastocysts derived from X-sorted sperm showed significantly (P < 0.05) lower quality in the prognostic factor (1) and combined (1) to (4) than that in unsorted sperm (35.3 v. 54.0 and 14.7 v. 42.9%, respectively). Pregnancy rates were higher for the blastocysts that had a high score in the prognostic factors (1) to (4) compared to those that had a low score (75.0%, n = 8 v. 36.4%, n = 22). These results suggest that quality of blastocysts, based on the prognostic factors studied, derived from X-sorted sperm is lower than that from unsorted sperm. Supported by the Research and Development projects for application in promoting new policy of agriculture, forestry and fisheries (22016).
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Clark, K., J. N. Drum, J. A. Rizo, and M. S. Ortega. "63 Effect of sire conception rate on bovine early embryo development." Reproduction, Fertility and Development 33, no. 2 (2021): 138. http://dx.doi.org/10.1071/rdv33n2ab63.
Full textAbstract:
Currently, the only measure of sire fertility in the bovine is sire conception rate (SCR), which is determined by Day 70 pregnancy diagnosis and not reflective of early embryo development. Therefore, this study aimed to establish the relationship between SCR and early embryo development. In the first experiment, 65 sires of negative (<−1, n=25), average (−1 to 1, n=19), and high (> +1, n=21) SCR were characterised for their ability to produce embryos using an invitro embryo production (IVP) system. For each sire, 100 cumulus–oocyte complexes (COCs) were used. COCs were matured for 22h, fertilized by co-incubation with sperm selected from density gradient centrifugation for 18h, and then placed in culture medium. A sire of known IVP performance was used as a control in each run. Cleavage and blastocyst rates (BL) were measured on Days 3 and 8 post-insemination, respectively. Photographs were taken on Days 3, 5, and 8 to identify arrest stages of non-blastocyst embryos. Sires were ranked based on their blastocyst rate and grouped into quartiles for statistical analysis. Differences in BL were determined by ANOVA using sire, IVP run, and a sire×IVP run interaction. In addition, the correlation between SCR and BL was determined. All data were analysed using SAS software version 9.4 (SAS Institute Inc.). Mean BL between each quartile was significant (P<0.05), with rates ranging from 8 to 22% and 32 to 62% for the lowest and highest quartile, respectively. There was no correlation (P=0.90) between SCR and BL. Arrest stage was measured by subtracting the number of Day-8 blastocysts from, first, embryos that were morulas on Day 5, and then embryos that were 8- to 16-cell stage embryos on Day 5. This method is based on the assumption that embryos closer to the blastocyst stage on Day 5 are more likely to contribute to the Day 8 blastocyst population. The most frequent arrest stage was the 4- to 6-cell stage (39/52 sires). It has been shown that decreased rates of autophagy are associated with embryonic arrest at the 4- to 8-cell stage in humans, leading us to investigate this mechanism in the second experiment. Select high (n=3) and low (n=4) performing sires identified in experiment 1 were used to generate 4- to 6-cell embryos, and autophagy rates were measured using live immunofluorescence with CYTO-ID autophagy dye (n=20 embryos/sire). The mean fluorescent intensity of each embryo was divided by the number of cells within the embryo. Differences in autophagy between high and low sires were determined by ANOVA using SAS. Interestingly, low-performing sires had a significantly higher autophagy rates than high-performing sires (77.8±3.1 vs. 50.0±3.5). This could indicate that embryos produced with low-performing sires had higher levels of stress than their counterparts. In summary, the effect of sire on embryonic development seems to be independent of the SCR classification. The most common arrest stage observed is the 4- to 6-cell stage, right before embryonic genome activation. Further research is required to elucidate the mechanisms by which sires influence pre-implantation development. This research was supported by USDA-NIFA AFRI Competitive Grant No. 2019-67015-28998.
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Clark, K., J. N. Drum, J. A. Rizo, and M. S. Ortega. "63 Effect of sire conception rate on bovine early embryo development." Reproduction, Fertility and Development 33, no. 2 (2021): 138. http://dx.doi.org/10.1071/rdv33n2ab63.
Full textAbstract:
Currently, the only measure of sire fertility in the bovine is sire conception rate (SCR), which is determined by Day 70 pregnancy diagnosis and not reflective of early embryo development. Therefore, this study aimed to establish the relationship between SCR and early embryo development. In the first experiment, 65 sires of negative (<−1, n=25), average (−1 to 1, n=19), and high (> +1, n=21) SCR were characterised for their ability to produce embryos using an invitro embryo production (IVP) system. For each sire, 100 cumulus–oocyte complexes (COCs) were used. COCs were matured for 22h, fertilized by co-incubation with sperm selected from density gradient centrifugation for 18h, and then placed in culture medium. A sire of known IVP performance was used as a control in each run. Cleavage and blastocyst rates (BL) were measured on Days 3 and 8 post-insemination, respectively. Photographs were taken on Days 3, 5, and 8 to identify arrest stages of non-blastocyst embryos. Sires were ranked based on their blastocyst rate and grouped into quartiles for statistical analysis. Differences in BL were determined by ANOVA using sire, IVP run, and a sire×IVP run interaction. In addition, the correlation between SCR and BL was determined. All data were analysed using SAS software version 9.4 (SAS Institute Inc.). Mean BL between each quartile was significant (P<0.05), with rates ranging from 8 to 22% and 32 to 62% for the lowest and highest quartile, respectively. There was no correlation (P=0.90) between SCR and BL. Arrest stage was measured by subtracting the number of Day-8 blastocysts from, first, embryos that were morulas on Day 5, and then embryos that were 8- to 16-cell stage embryos on Day 5. This method is based on the assumption that embryos closer to the blastocyst stage on Day 5 are more likely to contribute to the Day 8 blastocyst population. The most frequent arrest stage was the 4- to 6-cell stage (39/52 sires). It has been shown that decreased rates of autophagy are associated with embryonic arrest at the 4- to 8-cell stage in humans, leading us to investigate this mechanism in the second experiment. Select high (n=3) and low (n=4) performing sires identified in experiment 1 were used to generate 4- to 6-cell embryos, and autophagy rates were measured using live immunofluorescence with CYTO-ID autophagy dye (n=20 embryos/sire). The mean fluorescent intensity of each embryo was divided by the number of cells within the embryo. Differences in autophagy between high and low sires were determined by ANOVA using SAS. Interestingly, low-performing sires had a significantly higher autophagy rates than high-performing sires (77.8±3.1 vs. 50.0±3.5). This could indicate that embryos produced with low-performing sires had higher levels of stress than their counterparts. In summary, the effect of sire on embryonic development seems to be independent of the SCR classification. The most common arrest stage observed is the 4- to 6-cell stage, right before embryonic genome activation. Further research is required to elucidate the mechanisms by which sires influence pre-implantation development. This research was supported by USDA-NIFA AFRI Competitive Grant No. 2019-67015-28998.
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Kim, M. J., H. J. Oh, J. E. Park, S. G. Hong, J. E. Kim, G. Jang, T. A. Kim, M. S. Kwon, and B. C. Lee. "63 ESTABLISHMENT OF GREEN FLUORESCENT PROTEIN EXPRESSED DOG CELL LINES CONTROLLED BY DOXYCYCLINE." Reproduction, Fertility and Development 22, no. 1 (2010): 190. http://dx.doi.org/10.1071/rdv22n1ab63.
Full textAbstract:
An inducible gene expression system in transgenic animals has been widely used in biomedical science. The aim of this study was to establish green fluorescent protein (GFP) inducible dog cell line and evaluate the system in embryos using interspecies somatic cell nuclear transfer (iSCNT). Canine fetal fibroblasts were transfected with retroviral vector containing GFP, rtTA, and TRE and designated Gteton cell line. For iSCNT, bovine ovaries were collected from a local slaughterhouse and COCs were matured for 24 h. The denuded oocytes were enucleated, injected with Gteton cells, treated with 24 h of doxycycline (DOX), and electrically fused (NEPA GENE, 34 V, 15 μs, 2 pulses). The reconstructed oocytes were activated and then cultured in modified SOF medium. To verify the stability of the Gteton cells, 2 experiments were designed. Experiment 1 was designed to compare the cell size and viability of Gteton and nontransfected cells. Countness™ (Invitrogen, version 1.0, Carlsbad, CA, USA) was used for analysis. In experiment 2, the control of GFP gene expression was observed when the cells were cultured with 1 mg mL-1 of DOX. The cells were also cultured without DOX after 24 h of DOX treatment. Photographs were taken of cultured cells every 12 h. The intensity of GFP expression was analyzed by using Image J freeware (U.S. National Institutes of Health, version 1.42, NIH, Bethesda, MD, USA). To evaluate the reprogramming ability of the Gteton cells in embryos, another 2 experimental designs were planned. Experiment 3 estimated GFP expression in iSCNT embryos when they were cultured with and without DOX. Experiment 4 assessed the development of the iSCNT embryos under microscopy. Data were analyzed using statistical analysis system program (version 9.1, SAS Institute, Cary, NC, USA). In experiment 1, there was no significance (P < 0.05) in average viable cell size (13.7 v. 13.2 μm) or viability (97.0 v. 98.7%). In experiment 2, the GFP intensity increased steadily when cultured in medium containing DOX. The intensity was increased approximately two times after 24 h compared with 12 h of treatment. The intensity after 24 h of DOX treatment decreased to the basal level after 5 days. In experiment 3, the GFP intensity of iSCNT embryos cultured in mSOF containing DOX was increased approximately two times in 16-cell stage compared with 2-cell stage. In experiment 4, the cleavage rate was not significantly different between the 2 groups. In conclusion, we dtermined that the inducible system of Gteton cell line was established in a stable manner. Furthermore the results from iSCNT may indicate the possibility to produce GFP-expressed transgenic puppies controlled by doxycyline. This study was supported by Korean MEST through KOSEF (grant # M10625030005-09N250300510) and BK21 program, RNL BIO, and Natural Balance Korea.
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Griswold, Dylan, Halima Tabani, Ali Tayebi Meybodi, and Arnau Benet. "Using 3D Neuroanatomy Educational Resources as a Neurosurgical Teaching Tool in LMICs." Journal of Global Oncology 2, no. 3_suppl (June 2016): 27s. http://dx.doi.org/10.1200/jgo.2016.004333.
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Abstract 41 One of the Millennium Development Goals is to reduce childhood mortality; over 200,000 children currently develop cancer worldwide each year. 80% of those children live in Lower-Middle Income Countries (LMICs), which account for 90% of deaths (1). Nervous system (CNS) tumors are the most frequent solid tumors in children and adolescents (2). However, the median number of neurosurgeons per 100,000 population across different income groups is extremely variable (range: 0.01 - >1). In East Africa only 27 neurosurgeons are available to treat 270 million people, a 1:10 million ratio of neurosurgeons to inhabitants. Many countries do not even have one neurosurgeon (3). Even in low-resource areas where neurosurgery is an option, the high complexity of care for brain tumors often leads to suboptimal outcomes (4). As outlined by Rodriguez-Galindo et al., one of the first steps to improving outcomes in pediatric oncology is to both improve access to high-quality education as well as educational resources (1). Therefore, in order to improve access to neurosurgical educational resources we have generated a virtual library of 25,000 3D neurosurgical anatomical photographs (60% microsurgical, 40% endoscopic) and we have recorded 180 skull base and cerebrovascular cases in 3D in the operating room. In addition, we have presented the setup for producing a training operating room for residents and junior faculty to practice their surgical skills. With increasing opportunities to access and upload large amounts of data to cloud based servers, the surgical classroom of the future, especially in areas without the necessary personnel to train neurosurgical residents, may consist of a library of nearly every surgical approach recorded in three dimensions. This technology not only provides the student with the opportunity to develop a more thorough anatomical basis of key landmarks and features within the surgical window as compared to current 2D educational resources, but allows efficient self-learning. With the reported method, experienced neurosurgeons of the developed world can capture and build into this 3D virtual library to generate a global effort to improve neurosurgical education worldwide. Rodriguez-Galindo C, Friedrich P, Morrissey L, et al. Global challenges in pediatric oncology. Curr Opin Pediatr 2013; 25:3-15. Ezzat S, Kamal M, El-Khateeb, N, et al. Pediatric brain tumors in a low/middle income country: does it differ from that in developed world. Neuro-oncology 2015; Oct 29. Fuller A, Tran T, Muhumuza M, et al. Building neurosurgical capacity in low and middle income countries. eNeurologicalSci 2016; 3:1–6. Qaddoumi I, Unal E, Diez B, et al. Web-based survey of resources for treatment and long-term follow-up for children with brain tumors in developing countries. Childs Nerv Syst 2011; 27:1957–1961. AUTHORS' DISCLOSURES OF POTENTIAL CONFLICTS OF INTEREST: No COIs from the authors.
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Polupan, Yu P., N. L. Rieznykova, Y. S. Vysochanskii, and S. V. Pryima. "THE STATE OF BROWN CARPATHIAN AND BROWN CAUCASIAN CATTLE BREEDS ON FAO PROJECT TCP/RER/3604 “CONSERVATION AND DEVELOPMENT OF DUAL-PURPOSE CATTLE BREEDS IN EASTERN EUROPE”." Animal Breeding and Genetics 60 (November 23, 2020): 125–34. http://dx.doi.org/10.31073/abg.60.16.
Full textAbstract:
During 2018–2019 Ukraine took part in the project of Food and Agriculture Organization (FAO) «Conservation and development of double-purpose cattle breeds in Eastern Europe» (TCP/RER/3604). The project covered Brown Carpathian and Brown Caucasian breeds of Armenia, Georgia and Ukraine. On the results of the project, experts from the FAO Regional Office for Europe and Central Asia, international experts prepared reports on gender, social and economic characteristics of the studied regions, genomic analysis of samples and provided recommendations for breeding in the regions with the breeds. It was considered expedient to present these results to the general scientific community.
At each country FAO have chosen service-provider (organization), which should make survey on productive environment of targeted breed (in Ukraine – Brown Carpathian), conclude the general state of the breed, visit at least 100 farms where Caucasian/Carpathian Brown cattle breed was kept, collect information from 300 cows and 20 bulls, and genetic samples from at least 100 cows and 20 bulls kept for breeding. Service-provider in Ukraine was M.V.Zubets Institute of Animal Breeding and Genetics, NAAS.
Scientists of M.V.Zubets Institute of Animal Breeding and Genetics and other sub-contracting organizations during February-June, 2019 investigated 265 heads of cattle in 31 villages 5 districts of the Transcarpathian region, phenotypically classified as Brown Carpathian breed, including 6 breeding bulls of mating age (1.5–2 years). Each animal was photographed, linearly descriptive features of the exterior were evaluated, the necessary body measurements were taken with the help of appropriate tools, live weight and constitutional features were determined, and samples were taken for genetic testing.
A total of 554 hair samples were taken from the project (123 – from Armenia, 141 – from Georgia and 290 – from Ukraine).
The basis for classifying the animals as Brown Carpathian was phenotypic examination and strict compliance with the main characteristics of the breed: uniform grey or brown colour with different shades of it, general conformation typical for the breed, lighter belt along the back, black muzzle with lighter hair around it, black hooves and horn tips, lighter "glasses" around the eyes, thick light hair inside outer ears.
To form the genetic passport of the population, hair was selected from the tail switch of selected animals using equipment provided by FAO, sent to the laboratories of the International Atomic Energy Agency, Austria. In the laboratory in the district of Siebersdorf, DNA was isolated from the obtained samples, the genetic diversity of populations, the degree of inbreeding and their effective number, kinship between populations were assessed and the genetic structure and level of influence of other breeds were analysed.
DNA was extracted from each hair follicle using a MasterPure kit (Biozym, Illumina, USA) according to the protocol of the Association of Public Health Laboratories (APHL). The isolated DNA was subjected to a two-step quality control procedure before preparation for wide-genome typing. DNA samples were measured using a Nanodrop spectrophotometer to obtain a concentration with a ratio of 260/280 and 260/230. According to the results obtained using a spectrophotometer, DNA samples were diluted to measure high-quality double-stranded DNA (dsDNA) by PicoGreen Assay. The final working DNA was prepared based on PicoGreen measurements and stored at – 20°C for further processing.
Sample genotyping was performed using a third-generation 384-cell matrix to assess Bovine individuals on the Affymetrix-Axiom platform (Axiom Bovine Genotyping v3 array). Within the project, 48 individuals of Austrian Simmental and 18 – Ayrshire breeds were also genotyped for comparison. The matrix included 64.000 SNPs, providing a high probability of covering genomic variations of the Bos taurus and Bos indicus breeds.
Intra-breed genetic diversity was assessed using PLINK v1.09 to calculate actual heterozygosity (H0) and inbreeding coefficient (FIS).
Analysis of genetic parameters of population diversity evidenced a rather low inbreeding rate of Brown Carpathian breed and a fairly high effective population size compared to European breeds, which can indicate a very limited practice of artificial insemination and lack of selection among bulls.
Analysis of genetic differentiation and relationship between populations, principal components analysis and determination of admixtures of other breeds proved the uniqueness of the Brown Carpathian breed from a genetic point of view.
Based on the conducted genomic analysis of populations, consultations with project participants, reports of service providers of each country, an international consultant on animal genetic resources, chairman of the ICAR working group on functional traits, formed a breeding program for the studied populations. The breeding program included proposals for further selection work, calculation of basic selection-genetic parameters, selection effect, number of bulls and heifers for selection nucleus, number of doses to maintain genetic diversity of the population, cost of population restoration, breeding work of breeding centre and work plan by years to implement a breeding program.
Gender analysis of service providers' reports showed some gender differentiation in livestock care, processing and everyday life of owners of Brown Carpathian cattle.
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Tychsen, John, Ole Geertz-Hansen, and Frands Schjøth. "KenSea – tsunami damage modelling for coastal areas of Kenya." Geological Survey of Denmark and Greenland (GEUS) Bulletin 15 (July 10, 2008): 85–88. http://dx.doi.org/10.34194/geusb.v15.5051.
Full textAbstract:
On 26 December 2004, the eastern part of the Indian Ocean was hit by a tremendous tsunami created by a submarine earthquake of magnitude 9.1 on the Richter scale off the west coast of Sumatra. The tsunami also reached the western part of the Indian Ocean, including the coastal areas of eastern Africa. Along the coast of Kenya (Figs 1, 2) it resulted in a sudden increase in water level comparable to a high tide situation. This rather limited consequence was partly due to the great distance to the epicentre of the earthquake, and partly due to the low tide at the time of the impact. Hence the reefs that fringe two thirds of the coastline reduced the energy of the tsunami waves and protected the coastal areas. During the spring of 2005, staff members from the Geo- logical Survey of Denmark and Greenland (GEUS) carried out field work related to the project KenSea – development of a sensitivity atlas for coastal areas of Kenya (Tychsen 2006; Tychsen et al. 2006). Local fishermen and authorities often asked what would have been the effect if the tsunami had hit the coastal area during a high tide, and to answer the question GEUS and the Kenya Marine and Fisheries Research Institute (KMFRI) initiated a tsunami damage projection project. The aim was to provide an important tool for contingency planning by national and local authorities in the implementation of a national early warning strategy. The tsunami damage projection project used the database of coastal resources – KenSeaBase – that was developed during the KenSea project. The topographical maps of Kenya at a scale of 1:50 000 have 20 m contour lines, which is insufficient for the tsunami run-up simulation modelling undertaken by the new tsunami project. Therefore new sets of aerial photographs were obtained, and new photogrammetric maps with contour lines with an equidistance of 1 m were drawn for a 6–8 km broad coastal zone. The tsunami modelling is based on the assumption that the height of a future tsunami wave would be comparable with the one that reached the coastal area of Kenya in December 2004. Based on the regional geology of the Indian Ocean, it appears that the epicentre for a possible future earthquake that could lead to a new tsunami would most likely be situated in the eastern part of the ocean. Furthermore, based on a seismological assessment it has been estimated that the largest tsunami that can be expected to reach eastern Africa would have a 50% larger amplitude than the 2004 tsunami.It was therefore decided to carry out the simulation modelling with a tsunami wave similar to that of the 2004 event, but with the wave reaching the coast at the highest astronomical tide (scenario 1) and a worst case with a 50% larger amplitude (scenario 2: Fig. 3). The 2004 tsunami documented that the coastal belt of mangrove swamps provided some protection to the coastline by reducing the energy of the tsunami. Hence we included in this study a scenario 3 (Fig. 4), in which the mangrove areas along the coastline were removed. Maps for the three scenarios have been produced and show the areas that would be flooded, the degree of flooding, and the distribution of buildings such as schools and hospitals in the flooded areas. In addition, the force and velocity of the wave were calculated (COWI 2006).
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Cankul, Duran, Ozlem Peksen Ari, and Bendegul Okumus. "The current practices of food and beverage photography and styling in food business." Journal of Hospitality and Tourism Technology ahead-of-print, ahead-of-print (January 4, 2021). http://dx.doi.org/10.1108/jhtt-02-2020-0052.
Full textAbstract:
Purpose This study aims to analyze the current practices of food and beverage (F&B) photography and styling through the eyes of professionals involved in the food business. Design/methodology/approach A phenomenological research approach was used and empirical data were collected via a series of semi-structured interviews with professional food photographers. Purposive and snowballing sampling methods were used and the participants of this study consisted of 26 F&B photographers and stylists who were actively and professionally working in this field. Content analysis was applied to the empirical data. Findings The research findings reveal several key themes in F&B photography and styling: emotional appeal, key trends, career opportunities, required skills and abilities and interventions and ethical considerations. Three specific roles were identified in F&B photography and styling: food and beverage photographer, food and beverage stylist and prop stylist. Research limitations/implications This study offers discussions on the pros and cons of F&B photography beginners and professionals and the food business. Originality/value This is one of the first studies to offer empirical findings and discussions on the concept of F&B photography and styling.
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Pearce, Hanne. "A Fine Dessert: Four Centuries, Four Families, One Delicious Treat by S. Jenkins." Deakin Review of Children's Literature 6, no. 1 (July 28, 2016). http://dx.doi.org/10.20361/g20p5f.
Full textAbstract:
Jenkins, Sophie. 2015. Illus. Sophie Blackall. A Fine Dessert: Four Centuries, Four Families, One Delicious Treat. New York: Swartz & Wade Books. Print.A Fine Dessert is about “blackberry fool” a delicious dessert made from fresh blackberries and whipped cream. The story starts out in England in 1710 when a young girl and her mother pick blackberries with their hands. They have to milk the cows to get cream and whip it by hand with bundle of soft twigs. Then we are transported to Charleston, South Carolina in 1810 when another young girl and her mother pick blackberries from the garden at a plantation. A deliveryman brings them cream by horse and buggy and they must use a metal whisk by hand to get it whipped. Then we are in Boston in 1910 and a young girl and her mother buy their blackberries from the market. The deliveryman brings them cream and they use a mechanical whisk and ice box to make their dessert. Finally we are transported to San Diego and it is 2010. A young boy and his dad go to the supermarket to buy their blackberries. They look the recipe up on the Internet and use an electric mixer and refrigerator. Through one dessert, we see cooking and family life evolve over four hundred years.The concept of A Fine Dessert is brilliant as it presents an easy activity (cooking) that is mutual to all the centuries it depicts. The illustrations by Sophie Blackall are beautiful and tell their own story alongside the text. Period details and evocative facial expressions make these historical people come alive with elegant simplicity. This book is a fantastic way to teach history to children as it shows how even a mundane task such as cooking has changed over the centuries. While the story is about making a dessert there is so much more to the story. It opens the door to discussing social status, issues like slavery and gender roles. The book includes a recipe for blackberry fool and historical notes on the author’s research.Highly recommended: 4 out of 4 starsReviewer: Hanne PearceHanne Pearce has worked at the University of Alberta Libraries since 2004. Aside from being an avid reader, she has continuing interests in writing, photography, graphic design and knitting.
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Harmen, Harmen. "Diversity of plants as food supplement and medicine for livestock: Local culture in cattleman communities." Asian Journal of Ethnobiology 3, no. 1 (May 24, 2020). http://dx.doi.org/10.13057/asianjethnobiol/y030104.
Full textAbstract:
Abstract. Diversity of plants as food supplement and medicine for livestock: Local culture in cattleman communities. Asian J Ethnobiol 3: 23-29. Various types of plants can be used as food for livestock. In addition to its function as feed, several types of plants can also be used as medicines for livestock. The research aims to record the types of plant species that were traditionally used by farmers as feed supplements and medicines for livestock. Observations were made using a survey method in three regions in West Sumatra, namely Tanah Datar, Solok, and Limapuluh Kota District. Sampling was carried out randomly (random sampling) by picking up breeders who were considered as successful breeders (having more than five cows), and familiar with traditional medicines as a sample. Interviews were conducted to obtain information on the types of plants used as supplements or medicines. Furthermore, the plant types were recorded in their location of growing. All types of plants were documented in the form of photographs. Data were displayed descriptively, i.e., images with supporting information. The observations found more than 15 types of plants commonly used by cattlemen to increase growth, reproductive capacity, and as medicine if livestock had health problems.