Log in

Relevant bibliographies by topics / Photosynthesis Plant proteins / Dissertations / Theses

To see the other types of publications on this topic, follow the link: Photosynthesis Plant proteins.

Dissertations / Theses on the topic 'Photosynthesis Plant proteins'

Author: Grafiati

Published: 4 June 2021

Last updated: 30 January 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 36 dissertations / theses for your research on the topic 'Photosynthesis Plant proteins.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.

1

Hendry, Garth S. "Dependence of substrate-water binding on protein and inorganic cofactors of photosystem II /." View thesis entry in Australian Digital Theses Program, 2002. http://thesis.anu.edu.au/public/adt-ANU20041124.140348/index.html.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Hansson, Maria. "Molecular characterization of protein phosphorylation in plant photosynthetic membranes." Doctoral thesis, Linköping : Linköping University, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-6665.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Pwee, Keng-Hock. "Cis- and trans-acting elements involved in pea platocynanin gene expression." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239560.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Heurtel, Thuswaldner Sophie. "Nucleotide-binding Proteins in the Plant Thylakoid Membrane." Licentiate thesis, Linköping Department of Biomedicine and Surgery, Linköping University, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-7934.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Garcia, Cerdan Jose Gines. "Functions of REP27 and the low molecular weight proteins PsbX and PsbW in repair and assembly of photosystem II." Doctoral thesis, Umeå universitet, Kemi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-19517.

Full text

Abstract:

Oxygenic photosynthesis is the major producer of both oxygen and organic compounds on earth and takes place in plants, green algae and cyanobacteria. The thylakoid membranes are the site of the photosynthetic light reactions that involve the concerted action of four major protein complexes known as photosystem II (PSII), cytochrome b6f complex, ATP synthase and photosystem I (PSI). The function of PSII is of particular interest as it performs the light–driven water splitting reaction driving the photosynthetic electron transport. My thesis addressed different aspects of PSII assembly and the f

APA, Harvard, Vancouver, ISO, and other styles

6

Hernández-Prieto, Miguel Angel. "The Small Cab-like Proteins in the cyanobacterium Synechocystis sp. PCC 6803." Doctoral thesis, Umeå universitet, Kemi, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-25886.

Full text

Abstract:

The Small Cab-like Proteins (SCPs) in the cyanobacterium Synechocystis sp. PCC 6803 accumulate in cells grown under different stress conditions. Genes coding for SCPs have been found in all sequenced organisms performing oxygenic photosynthesis and even in the genomes of cyanophages. Deletion of multiple scp genes in Synechocystis resulted in mutants with severely impaired growth and altered pigment content. These findings indicate the importance of SCPs in photosynthesis; however, their specific function is not well understood. SCPs share a chlorophyll-binding motif with the plant light harve

APA, Harvard, Vancouver, ISO, and other styles

7

Kuntz, Marcel. "Etude de la structure et de l'expression des genomes plastidiaux et du transport des proteines dans les chloroplastes." Université Louis Pasteur (Strasbourg) (1971-2008), 1989. http://www.theses.fr/1989STR13012.

Full text

Abstract:

Etude des genes de trna d'organistes photosynthetiques et determination des sequences nucleotidiques des genes du trna**(t4r)(ggu), trna**(glu)(uuc) et trna**(tyr)(gua) chloroplastiques de feve et des genes des trna**(glu)(uuc), trna**(leu)(uaa), trna**(ser)(gga) et trna**(gly)(ggc) des cyanelles de cyanophora paradoxa. Un intron de classe i a ete mis en evidence dans le gene codant pour le trna**(leu)(uaa) de cyanelles. A l'aide des techniques de fusion de genes et de transformation des plantes, il est apparu que le peptide transit de la pente sous unite de la rubisco permet le transport effi

APA, Harvard, Vancouver, ISO, and other styles

8

Fristedt, Rikard. "Regulatory Functions of Protein Phosphorylation in Plant Photosynthetic Membranes." Doctoral thesis, Linköpings universitet, Cellbiologi, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-62303.

Full text

Abstract:

Oxygenic photosynthesis is the process in plants, algae and cyanobacteria which converts light energy from the sun into carbohydrates and at the same time produces oxygen from water. Both carbohydrates and oxygen are essential to sustain life on earth. Sunlight is thus a necessity for life, but it can also cause severe problems for photosynthetic organisms, which have evolved several remarkable acclimation systems to cope with light fluctuations in the environment. In higher plants the light driven reactions of photosynthesis proceed in the chloroplast thylakoid membranes highly organized into

APA, Harvard, Vancouver, ISO, and other styles

9

Shutova, Tatiana. "Photosynthetic water oxidation : the function of two extrinsic proteins." Doctoral thesis, Umeå : Department of Plant Physiology, Umeå Univ, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1476.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Campos, Gorgona Nefertiti. "Aislamiento y caracterización del gen de la transglutaminasa de arroz (Oryza sativa)." Doctoral thesis, Universitat de Barcelona, 2013. http://hdl.handle.net/10803/124484.

Full text

Abstract:

En esta tesis se describen los resultados obtenidos en la clonación y caracterización de una nueva transglutaminasa (TGasa) vegetal aislada en arroz (Oryza sativa), la segunda transglutaminasa clonada en plantas y la primera en plantas C3. Las TGasas (E.C.2.3.2.13) son una familia de enzimas que promueven modificaciones postraduccionales de proteínas. Tienen especial importancia debido a sus implicaciones en enfermedades humanas y a sus aplicaciones biotecnológicas de interés industrial. Su investigación en plantas es aún incipiente y hasta la fecha, solo se ha clonado la TGasa cloroplásti

APA, Harvard, Vancouver, ISO, and other styles

11

Thompson, Elinor Patsy. "Proteins involved in the maintenance of the photosynthetic apparatus in cyanobacteria and plants." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406077.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Ajigboye, Olubukola Omowunmi. "The role of the protein PGR5 in photoprotection and photosynthetic productivity in rice (Oryza sativa L.)." Thesis, University of Nottingham, 2013. http://eprints.nottingham.ac.uk/28540/.

Full text

Abstract:

In plants, the need to efficiently respond to constant environmental fluctuations has led to the evolution of photoprotective mechanisms to regulate photosynthetic light reactions. One of such mechanism is cyclic electron transport (CET) which generates a pH gradient inducing the nonphotochemical quenching (NPQ) of excess excitation energy, synthesis of ATP and maintenance of balanced redox state of the photosynthetic electron transport (PET) chain. Two major pathways of CET involve the NADH dehydrogenase-like complex (NDH)-dependent pathway, and the Proton Gradient Regulation 5 (PGRS)-depende

APA, Harvard, Vancouver, ISO, and other styles

13

Leonardo, Basso. "Optimization of accelerator and brake in photosynthetic electron transport." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263509.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Xia, Hanhan. "Influence of the light harvesting proteins Lhbc3 and Lhbc5 on photosynthesis of plants lacking Lhbc1 and Lhbc2." Thesis, Umeå universitet, Institutionen för fysiologisk botanik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-89185.

Full text

Abstract:

Photosynthesis is one of the most important biological processes on earth, producing carbohydrates and oxygen. The light is captured with the aid of light harvesting proteins. Light harvesting complex proteins in PSII are important both in light absorption and dynamic regulation. This study aims to confirm the individual role of Lhcb1 and Lhcb2 in the regulation of photosynthesis based on the KO lines: koLhcb3, koLhcb5 and koLhcb3koLhcb5. By the tool of western blot, phosphorylation analysis, state transition and non-photochemical quenching, the results indicated that the knock-out of Lhcb3, L

APA, Harvard, Vancouver, ISO, and other styles

15

Viola, Stefania [Verfasser], and Dario Michael [Akademischer Betreuer] Leister. "Expression of higher plant photosynthetic proteins in the cyanobacterium Synechocystis sp. PCC 6803 / Stefania Viola. Betreuer: Dario Leister." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2014. http://d-nb.info/1082205362/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Viola, Stefania Verfasser], and Dario Michael [Akademischer Betreuer] [Leister. "Expression of higher plant photosynthetic proteins in the cyanobacterium Synechocystis sp. PCC 6803 / Stefania Viola. Betreuer: Dario Leister." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2014. http://d-nb.info/1082205362/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Mohamed, Gadija. "The effects of gallic acid on the membrane proteome and antioxidant system of wheat plants under salt stress." University of Western Cape, 2020. http://hdl.handle.net/11394/8252.

Full text

Abstract:

>Magister Scientiae - MSc<br>Salt stress is a major abiotic stress that accounts for huge agricultural losses worldwide, which in turn threaten food security and sustainable agriculture. Salt triggers the excessive production of reactive oxygen species (ROS) which accumulate to levels that become toxic to plants, resulting in cell death and reduced plant growth. Part of the plant’s mechanisms to counteract ROS-induced cell death involves the scavenging ability of the antioxidant defense system to maintain redox homeostasis. Gallic acid (GA) is an antioxidant that has been shown to reduce salt-

APA, Harvard, Vancouver, ISO, and other styles

18

Frigerio, Sara. "Plant response to abiotic stress : analysis of changes in the photosynthetic apparatus at both gene and protein level." Aix-Marseille 2, 2008. http://www.theses.fr/2008AIX22076.

Full text

Abstract:

In the first part of the work the behaviour of different light harvesting complex (Lhc) subunits, belonging to a highly conserved multigenic family, was analysed in response to different growth conditions in Zea mays. The redundancy of these sequences suggested, in fact, a possible specific role of each gene product in light harvesting and photoprotection, depending on environmental conditions. Plants were grown in different conditions of light and temperature and thylakoid membranes were isolated in order to test the accumulation of different Lhc proteins. Significant differences were found i

APA, Harvard, Vancouver, ISO, and other styles

19

Vasquez-Robinet, Cecilia. "Relationships Between Expression of Heat Shock Protein Genes and Photosynthetic Behavior During Drought Stress in Plants." Diss., Virginia Tech, 2007. http://hdl.handle.net/10919/27009.

Full text

Abstract:

Heat shock proteins (HSPs) are expressed in response to environmental stresses. Compared to other kingdoms, plant HSP families are larger, presumably the result of adaptation to a wide range of stresses. Following on an analysis of drought stress characteristics in loblolly pine (Watkinson et al., 2003), expression patterns of HSP gene expression during photosynthetic acclimation were examined. One cycle of mild (-1Mpa) followed by two cycles of severe stress (-1.7Mpa) were probed for conditioning effects. Photosynthetic acclimation occurred after the first cycle. No acclimation occurred witho

APA, Harvard, Vancouver, ISO, and other styles

20

Crouchman, Sophie Jane. "Role of the PsbS protein in protective energy dissipation in the photosynthetic membrane in higher plants." Thesis, University of Sheffield, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440942.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Singh, Asmita. "Illuminating the ultrafast excited state dynamics of protein-bound carotenoids in plants." Diss., University of Pretoria, 2017. http://hdl.handle.net/2263/63170.

Full text

Abstract:

Global energy demands have escalated over the past few decades, creating a necessity for alternative energy sources. Solar technologies inspired by the primary solar energy storing process known on earth, photosynthesis, have subsequently gained popularity. The natural photosynthetic apparatus comprises a network of membrane-bound pigment-protein complexes, with the main plant light-harvesting complex (LHCII) consisting of chlorophyll (Chl) and carotenoid (Car) pigments. Electronic excitation energy transfer (ET) of the harvested energy takes place amongst these pigments on ultrafast timescale

APA, Harvard, Vancouver, ISO, and other styles

22

Schubert, Axel. "Studying nonlinear optical properties of the plant light harvesting protein LHCII." Doctoral thesis, [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972710507.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Jiang, Lei [Verfasser]. "Folding and assembly of the major light-harvesting chlorophyll protein (LHCII) of the photosynthetic apparatus in plants : the chlorophyll a - containing intermediate / Lei Jiang." Mainz : Universitätsbibliothek der Johannes Gutenberg-Universität Mainz, 2016. http://d-nb.info/122529634X/34.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Karbaschi, Mohammad Reza. "Structural, physiological and molecular characterisation of the Australian native resurrection grass Tripogon loliiformis (F.Meull) C.E.Hubb during dehydration and rehydration." Thesis, Queensland University of Technology, 2015. https://eprints.qut.edu.au/90050/1/Mohammad%20Reza_Karbaschi_Thesis.pdf.

Full text

Abstract:

There is an urgent need to develop crops that can withstand future climates. Results from this thesis demonstrated that a native Australian resurrection grass exhibits structural, physiological and metabolic strategies to tolerate drying. These strategies may be utilized for the generation of stress tolerant crops.

APA, Harvard, Vancouver, ISO, and other styles

25

Orr, Douglas John. "Plant Rubisco biogenesis : a study of the requirements and processing steps during co- and post-translational modification of the large subunit." Phd thesis, 2012. http://hdl.handle.net/1885/155151.

Full text

Abstract:

The production of chemical energy from light energy and C0{u2082} by photosynthesis is essential for life in the biosphere, and thus so is the activity of the often rate limiting CO{u2082}-fixing enzyme Rubisco (D-ribulose-l,5-bisphosphate carboxylase/oxygenase). The importance of this 'gateway' enzyme for carbon entry into the biosphere, and it's apparent inherent flaws in catalysis, have made it a key target for improvement as a key step towards enhancing photosynthesis and agricultural yield potential. For example, the diminished carboxylation efficiency and C0{u2082}/0{u2082} specificity o

APA, Harvard, Vancouver, ISO, and other styles

26

Zhou, Yu. "Exploring the evolutionary protein landscape of red Rubisco towards improving plant photosynthesis." Phd thesis, 2021. http://hdl.handle.net/1885/256570.

Full text

Abstract:

Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) conducts the carbon entry into the biosphere by fixing the CO2 into organic carbon. Rubisco is the most abundant enzyme on the Earth because it prevalently exists in plants, cyanobacteria, algae, bacteria and Archaea. Rubisco has the major defaults in its structure and then results in its slow and error-prone catalytic properties, but some red Rubisco have been characterised are more efficient. Using directed evolution approaches in the foreign host has been successfully improved the catalytic properties and functional assembly of some

APA, Harvard, Vancouver, ISO, and other styles

27

Chao, Hsien-Yu, and 趙晛羽. "Plant Ferredoxin-like Protein (pflp) Transgenic Rice Exhibits Higher Photosynthesis Activity under Salt Stress." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/50075360376493104970.

Full text

Abstract:

碩士<br>國立高雄大學<br>生物科技研究所<br>100<br>Photosynthesis is the major biochemical pathway that assimilates carbon dioxide (CO2) into organic compounds for plant growth. Plant ferredoxin-like protein (PFLP) was isolated from sweet peppers, shows high homology to the Fd-I sequence of Arabidopsis thaliana, Lycopersicon esculentum, Oryza sativa and Spinacia oleracea. Previous studies show that constitutive expression of the pflp in transgenic plants rice TNG67 and Phalaenopsis can enhance photosynthetic efficiency. Salinity is a major abiotic stress factor limiting growth and productivity of crops in irri

APA, Harvard, Vancouver, ISO, and other styles

28

Pournia, Farnaz. "Effects of Cadmium on Morphology, Photosynthesis and Protein Profile of Bean Plants." 2009. http://hdl.handle.net/2429/6047.

Full text

Abstract:

Cadmium (Cd), a cancer-causing heavy metal ion, concentration in the environment has recently shown a rapid increase mainly as a result of human activity. Since many plants are used as medicine and food, it is crucial to investigate effects of Cd on plant growth and development. This study examines the time course and concentration-dependent effects of Cd on morphology, photosynthesis and protein profiles in hydroponically-grown bean plants (Phaseolus vulgaris). High concentrations of Cd (1000 and 100uM) induced leaf wilting and reduced both leaf growth and photosynthesis rate within 24 and 48

APA, Harvard, Vancouver, ISO, and other styles

29

Chen, Yen-Ting, and 陳彥廷. "Overexpressing Plant Ferredoxin-like Protein Enhances Photosynthetic Efficiency and Carbohydrates Accumulation in Phalaenopsis." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/43902503105323536029.

Full text

Abstract:

碩士<br>國立高雄大學<br>生物科技研究所<br>99<br>Crassulacean acid metabolism (CAM) is one of three major models of carbon dioxide assimilation pathway with better water-use efficiency and slower photosynthetic efficiency in photosynthesis. Previous studies indicate that expressing gene of sweet pepper plant ferredoxin-like protein (PFLP) can enhance disease resistance to bacterial pathogens. The protein sequence of PFLP shows high homology to the ferredoxin-1(Fd-1) family that belongs to photosynthetic type Fd and involves in photosystem I. It is proposed that overexpressing pflp in transgenic plant may enha

APA, Harvard, Vancouver, ISO, and other styles

30

"Life of Photosynthetic Complexes in the Cyanobacterium Synechocystis sp. PCC 6803." Doctoral diss., 2011. http://hdl.handle.net/2286/R.I.9181.

Full text

Abstract:

abstract: The cyanobacterium Synechocystis sp. PCC 6803 performs oxygenic photosynthesis. Light energy conversion in photosynthesis takes place in photosystem I (PSI) and photosystem II (PSII) that contain chlorophyll, which absorbs light energy that is utilized as a driving force for photosynthesis. However, excess light energy may lead to formation of reactive oxygen species that cause damage to photosynthetic complexes, which subsequently need repair or replacement. To gain insight in the degradation/biogenesis dynamics of the photosystems, the lifetimes of photosynthetic proteins and chlor

APA, Harvard, Vancouver, ISO, and other styles

31

Lim, Yi Leen. "Overcoming limitations in bioengineering Rubisco in higher plant chloroplasts." Phd thesis, 2015. http://hdl.handle.net/1885/111459.

Full text

Abstract:

Better structure-function studies of higher plant Rubisco are imperative in improving catalytic potential of the enzyme Rubisco. In this thesis, a novel system to study Rubisco using an RNAi tobacco genotype is designed to provide a homogenous environment of large and small Rubisco subunits for a more genuine assessment of recombinant Rubisco catalysis, regulation and assembly as well as its photosynthetic capacity in tobacco. The application of technology and strategies discussed in this thesis will demonstrate a great leap forward in Rubisco bioengineering and recombinant protein expression

APA, Harvard, Vancouver, ISO, and other styles

32

Dwyer, Simon Alexander Nicholas. "Photosynthetic consequences of impaired Photosystem II : antisense reductions in the PsbO proteins in Arabidopsis thaliana." Phd thesis, 2011. http://hdl.handle.net/1885/150904.

Full text

Abstract:

Photosystem (PS) II is the multi-sub unit complex which uses light energy to split water, providing the reducing equivalents needed for photosynthesis. The complex is susceptible to damage from environmental stresses such as excess excitation energy and high temperature. This thesis investigates the in vivo photosynthetic consequences of impairments to PSII in Arabidopsis thaliana expressing an antisense construct to the PsbO proteins of PSII. Transgenic lines were obtained with between 25 and 100% of wild type (WT) PsbO protein content per leaf area, and this decrease coincided with a proport

APA, Harvard, Vancouver, ISO, and other styles

33

Cheng, Chin-Fu, and 鄭執甫. "Constitutive Expression of a Plant Ferredoxin-like Protein (pflp) in Transgenic Rice Enhances Capacity of Photosynthetic Carbon Assimilation." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/64694469826539960451.

Full text

Abstract:

碩士<br>國立高雄大學<br>生物科技研究所<br>98<br>Photosynthesis is the major metabolic pathway that converts carbon dioxide (CO2) into organic compounds, such as fructose, glucose, sucrose, and starch in plant. Ferredoxin-I (Fd-I) protein, known as an electron carrier in photosynthesis, generally exists in green tissue. The protein sequence of the plant ferredoxin – like protein (PFLP), isolated from sweet peppers, shows high homology to the Fd-I sequence of Arabidopsis thaliana, Lycopersicon esculentum, Oryza sativa and Spinacia oleracea. Previous studies reported that constitutive expression of pflp in tran

APA, Harvard, Vancouver, ISO, and other styles

34

Mueller-Cajar, Oliver Martin. "Directed evolution of Rubisco in Escherichia coli." Phd thesis, 2006. http://hdl.handle.net/1885/149709.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Buczyńska, Alicja. "Charakterystyka chloroplastów z komórek mezofilu i komórek pochew okołowiązkowych roślin C4 podtypu NADP-ME w warunkach fotoinhibicji." Doctoral thesis, 2018. https://depotuw.ceon.pl/handle/item/3047.

Full text

Abstract:

Budowa aparatu fotosyntetycznego jest ściśle związana z warunkami świetlnymi w środowisku, a przystosowanie roślin do różnych i zmieniających się natężeń światła pociąga za sobą zmiany w strukturze i funkcjonowaniu kompleksów fotosyntetycznych. U roślin C4 proces fotosyntezy rozdzielony jest przestrzennie i zachodzi w dwóch typach komórek – mezofilu (M) i pochew okołowiązkowych (BS). Chloroplasty BS u roślin C4 podtypu NADPME mają budowę bezgranową lub posiadają szczątkowe grana, natomiast chloroplasty M są granowe. Nie wiadomo, jakie są mechanizmy odpowiedzialne za przystosowywanie się aparat

APA, Harvard, Vancouver, ISO, and other styles

36

BENEDIKTYOVÁ, Zuzana. "Imaging of fluorescence emission signals from healthy and infected leaf tissues." Doctoral thesis, 2009. http://www.nusl.cz/ntk/nusl-52374.

Full text

Abstract:

Auto-fluorescence emission of plant tissues can be a powerful reporter on plant biochemistry and physiology since it originates in substances inherent to primary or secondary metabolism. Plant bodies contain a plethora of intrinsic fluorescent compounds emitting practically all wavelengths of visible light. Moreover, the spectrum of fluorescent reporter signals was recently extended by a variety of fluorescent proteins that provide a new tool to mark whole cells or sub-cellular structures, study protein localization and monitor gene expression and molecule interactions. The imaging of such flu

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!