Academic literature on the topic 'Physiology of biofilms'

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Journal articles on the topic "Physiology of biofilms"

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Schulze, Adina, Fabian Mitterer, Joao P. Pombo, and Stefan Schild. "Biofilms by bacterial human pathogens: Clinical relevance - development, composition and regulation - therapeutical strategies." Microbial Cell 8, no. 2 (2021): 28–56. http://dx.doi.org/10.15698/mic2021.02.741.

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Notably, bacterial biofilm formation is increas-ingly recognized as a passive virulence factor facilitating many infectious disease processes. In this review we will focus on bacterial biofilms formed by human pathogens and highlight their relevance for diverse diseases. Along biofilm composition and regulation emphasis is laid on the intensively studied biofilms of Vibrio cholerae, Pseu-domonas aeruginosa and Staphylococcus spp., which are commonly used as biofilm model organisms and therefore contribute to our general understanding of bacterial bio-film (patho-)physiology. Finally, therapeut
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Vieira, Helena L. A., Patrick Freire, and Cecília M. Arraiano. "Effect of Escherichia coli Morphogene bolA on Biofilms." Applied and Environmental Microbiology 70, no. 9 (2004): 5682–84. http://dx.doi.org/10.1128/aem.70.9.5682-5684.2004.

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ABSTRACT Biofilm physiology is established under a low growth rate. The morphogene bolA is mostly expressed under stress conditions or in stationary phase, suggesting that bolA could be implicated in biofilm development. In order to verify this hypothesis, we tested the effect of bolA on biofilm formation. Overexpression of bolA induces biofilm development, while bolA deletion decreases biofilms.
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von Ohle, Christiane, Armin Gieseke, Laura Nistico, Eva Maria Decker, Dirk deBeer, and Paul Stoodley. "Real-Time Microsensor Measurement of Local Metabolic Activities in Ex Vivo Dental Biofilms Exposed to Sucrose and Treated with Chlorhexidine." Applied and Environmental Microbiology 76, no. 7 (2010): 2326–34. http://dx.doi.org/10.1128/aem.02090-09.

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ABSTRACT Dental biofilms are characterized by structural and functional heterogeneity. Due to bacterial metabolism, gradients develop and diverse ecological microniches exist. The aims of this study were (i) to determine the metabolic activity of microorganisms in naturally grown dental biofilms ex vivo by measuring dissolved oxygen (DO) and pH profiles with microelectrodes with high spatial resolution and (ii) to analyze the impact of an antimicrobial chlorhexidine (CHX) treatment on microbial physiology during stimulation by sucrose in real time. Biofilms were cultivated on standardized huma
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Campoccia, Davide, Lucio Montanaro, and Carla Renata Arciola. "Extracellular DNA (eDNA). A Major Ubiquitous Element of the Bacterial Biofilm Architecture." International Journal of Molecular Sciences 22, no. 16 (2021): 9100. http://dx.doi.org/10.3390/ijms22169100.

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After the first ancient studies on microbial slime (the name by which the biofilm matrix was initially indicated), multitudes of studies on the morphology, composition and physiology of biofilms have arisen. The emergence of the role that biofilms play in the pathogenesis of recalcitrant and persistent clinical infections, such as periprosthetic orthopedic infections, has reinforced scientific interest. Extracellular DNA (eDNA) is a recently uncovered component that is proving to be almost omnipresent in the extracellular polymeric substance (EPS) of biofilm. This macromolecule is eliciting un
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Steinberger, R. E., and P. A. Holden. "Extracellular DNA in Single- and Multiple-Species Unsaturated Biofilms." Applied and Environmental Microbiology 71, no. 9 (2005): 5404–10. http://dx.doi.org/10.1128/aem.71.9.5404-5410.2005.

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ABSTRACT The extracellular polymeric substances (EPS) of bacterial biofilms form a hydrated barrier between cells and their external environment. Better characterization of EPS could be useful in understanding biofilm physiology. The EPS are chemically complex, changing with both bacterial strain and culture conditions. Previously, we reported that Pseudomonas aeruginosa unsaturated biofilm EPS contains large amounts of extracellular DNA (eDNA) (R. E. Steinberger, A. R. Allen, H. G. Hansma, and P. A. Holden, Microb. Ecol. 43:416-423, 2002). Here, we investigated the compositional similarity of
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Wijeyekoon, S., T. Mino, H. Satoh, and T. Matsuo. "Growth and novel structural features of tubular biofilms produced under different hydrodynamic conditions." Water Science and Technology 41, no. 4-5 (2000): 129–38. http://dx.doi.org/10.2166/wst.2000.0436.

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Biofilm growth and internal structures were investigated by Confocal Scanning Laser Microscopy and fluorescently labeled oligonucleotide probe hybridization. Biofilms on smooth flat surfaces such as glass slides grew as isolated cell clusters. Under a hydraulic linear flow velocity of 1.7 cm/s mature biofilms exhibited a network like structure consisting of large interconnected cell clusters leading to possible three-dimensional mass transport. Smooth curved tube surfaces were also colonized by isolated cell clusters. However a clustered structure was not observed in mature tubular biofilms wh
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Frederick, Jesse R., James G. Elkins, Nikki Bollinger, Daniel J. Hassett, and Timothy R. McDermott. "Factors Affecting Catalase Expression in Pseudomonas aeruginosa Biofilms and Planktonic Cells." Applied and Environmental Microbiology 67, no. 3 (2001): 1375–79. http://dx.doi.org/10.1128/aem.67.3.1375-1379.2001.

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ABSTRACT Previous work with Pseudomonas aeruginosa showed that catalase activity in biofilms was significantly reduced relative to that in planktonic cells. To better understand biofilm physiology, we examined possible explanations for the differential expression of catalase in cells cultured in these two different conditions. For maximal catalase activity, biofilm cells required significantly more iron (25 μM as FeCl3) in the medium, whereas planktonic cultures required no addition of iron. However, iron-stimulated catalase activity in biofilms was still only about one-third that in planktoni
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Okabe, S., T. Kindaichi, Y. Nakamura, and T. Ito. "Eco-physiology of autotrophic nitrifying biofilms." Water Science and Technology 52, no. 7 (2005): 225–32. http://dx.doi.org/10.2166/wst.2005.0205.

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Microautoradiography combined with fluorescent in situ hybridization (MAR-FISH), a powerful tool for linking physiology with identification of individual cells, was applied to investigate microbial interactions between nitrifying bacteria and coexisting heterotrophic bacteria in an autotrophic nitrifying biofilm community fed with only ammonia as the sole energy source and bicarbonate as the sole carbon source. First, nitrifying bacteria were radiolabeled by culturing the biofilm samples with [14C]bicarbonate for 6 h, and then the transfer of radioactivity from nitrifying bacteria to heterotro
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Yuan, Lei, Fedrick C. Mgomi, Zhenbo Xu, Ni Wang, Guoqing He, and Zhenquan Yang. "Understanding of food biofilms by the application of omics techniques." Future Microbiology 16, no. 4 (2021): 257–69. http://dx.doi.org/10.2217/fmb-2020-0218.

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Biofilms constitute a protective barrier for foodborne pathogens to survive under stressful food processing conditions. Therefore, studies into the development and control of biofilms by novel techniques are vital for the food industry. In recent years, foodomics techniques have been developed for biofilm studies, which contributed to a better understanding of biofilm behavior, physiology, composition, as well as their response to antibiofilm methods at different molecular levels including genes, RNA, proteins and metabolic metabolites. Throughout this review, the main studies where foodomics
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Adams, Jennifer L., and Robert J. C. McLean. "Impact of rpoS Deletion onEscherichia coli Biofilms." Applied and Environmental Microbiology 65, no. 9 (1999): 4285–87. http://dx.doi.org/10.1128/aem.65.9.4285-4287.1999.

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ABSTRACT Slow growth has been hypothesized to be an essential aspect of bacterial physiology within biofilms. In order to test this hypothesis, we employed two strains of Escherichia coli, ZK126 (ΔlacZ rpoS +) and its isogenic ΔrpoS derivative, ZK1000. These strains were grown at two rates (0.033 and 0.0083 h−1) in a glucose-limited chemostat which was coupled either to a modified Robbins device containing plugs of silicone rubber urinary catheter material or to a glass flow cell. The presence or absence of rpoS did not significantly affect planktonic growth of E. coli. In contrast, biofilm ce
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Dissertations / Theses on the topic "Physiology of biofilms"

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Lethbridge, Samuel Aston. "Physiology and antibiotic susceptibility of mycobacterial biofilms." Thesis, St George's, University of London, 2018. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.754066.

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Treatment of tuberculosis requires months of antimycobacterial therapy. This tolerance to antibiotics displayed by Mycobacterium tuberculosis could be attributed to biofilm formation. Biofilms are the cause of many chronic infections. The aim of this thesis was to apply laboratory methods for the culture of Mycobacterium smegmatis and M. tuberculosis H37Rv biofilms and to further characterise these bacterial phenotypes in terms of their physiology, gene expression and drug susceptibility The Modified Robbins Device (MRD) and the Constant Depth Film Fermenter (CDFF) laboratory models were appli
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Gillan, David. "Morpho-physiology, genetics and ecological aspects of marine microbial biofilms :the study of the iron-encrusted biofilm associated with Montacuta ferruginosa (Mollusca, Bivalvia)." Doctoral thesis, Universite Libre de Bruxelles, 1999. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/211930.

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Hastings, Cody M. "Determination of the effects that a previously uncharacterized secreted product from Klebsiella pneumoniae has on Citrobacter freundii and Enterobacter cloacae biofilms." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/honors/419.

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More so than ever, Multiple Drug Resistant (MDR) bacteria are on the rise due to overuse of antibiotics along with natural selection for adaptations that enhance drug-resistant properties. One particular bacterial family, Enterobacteriaceae, has been problematic, exhibiting several bacterial members that have developed a precipitous resistance to modern antibiotics and are also primary causative agents of nosocomial, or hospital acquired, infections. Citrobacter freundii (CF) and Enterobacter cloacae (ECL) are two species of the Enterobacteriaceae family causing significant medical concern due
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Stark, Roger Matthew. "Studies on the growth and physiology of Helicobacter pylori." Thesis, University of the West of England, Bristol, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244291.

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Pfendler, Stéphane. "Intérêt du traitement par UV-C des communautés bactériennes, fongiques et des protistes autotrophes des biofilms colonisant la pierre patrimoniale : structure des peuplements, effets des UV-C sur la physiologie algale et innocuité du traitement vis à vis du support pictural." Thesis, Bourgogne Franche-Comté, 2017. http://www.theses.fr/2017UBFCD030/document.

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Dans le cadre de la conservation du patrimoine, les micro-organismes se développant sous forme de biofilm sont souvent considérés comme des agents entraînant des problèmes esthétiques et de détérioration du support colonisé. L’objectif général de cette thèse est l’utilisation de la lumière UV-C comme méthode de traitement des biofilms. Dans le but de comprendre la diversité des biofilms, les communautés bactériennes, fongiques et des protistes autotrophes ont été étudiées à l’aide d’une technique de séquençage haut débit. Puis, afin de comprendre les effets des UV-C sur les micro-algales (Chlo
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Gellé, Marie-Paule. "Physiologie des biofilms de streptocoques oraux : de la colonisation a la destructuration ; analyse microbiologique, physico-chimique et microscopique (doctorat : odontologie)." Reims, 1998. http://www.theses.fr/1998REIM0201.

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Khelissa, Simon Oussama. "Caractérisation des propriétés physiologiques associées aux cellules détachées de biofilms et étude des interactions aux interfaces entre bactéries et matériaux : cas de Staphylococcus aureus et Pseudomonas aeruginosa." Thesis, Lille 1, 2017. http://www.theses.fr/2017LIL10195.

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Le biofilm est à l’origine d’infections nosocomiales et d’intoxications alimentaires dans les secteurs alimentaire et hospitalier. Les bactéries structurées en biofilm peuvent se détacher et coloniser de nouvelles surfaces. Le risque microbiologique associé aux bactéries détachées de biofilm est peu étudié. Les travaux de thèse ont concerné, l’étude de l’effet des conditions de croissance sur les propriétés physicochimiques de surface de Staphylococcus aureus et Pseudomonas aeruginosa sous leurs formes détachées de biofilm et planctoniques, ainsi que leurs adhésion sur l'acier inoxydable (SS)
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Bzdrenga, Janek. "De la promiscuité des enzymes : cas des PLLs et de leur implication dans l'anti virulence bactérienne et la décontamination des organophosphorés." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM5018.

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Les PLLs sont une famille d’enzyme dont l’activité catalytique est double. L’activité lactonase permet entre autre de détruire les AHLs, molécules médiatrices de la communication chez les bactéries Gram négatives, empêchant ainsi la synchronisation à l’échelle de la population de la sécrétion de facteurs de virulence ou la formation de biofilm. Elles sont non seulement capables d’hydrolyser les molécules possédant un noyau lactone, mais aussi les phosphotriésters par promiscuité de substrat. L’activité phosphotriestérase permet de dégrader les composés organophosphorés (OPs) hautement toxiques
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Carvalhais, Virgínia Maria Dinis. "Effect of modulating S. Epidermidis biofilms dormancy in the biofilm physiology and host immune response." Tese, 2016. https://repositorio-aberto.up.pt/handle/10216/100331.

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Carvalhais, Virgínia Maria Dinis. "Effect of modulating S. Epidermidis biofilms dormancy in the biofilm physiology and host immune response." Doctoral thesis, 2016. https://repositorio-aberto.up.pt/handle/10216/100331.

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Books on the topic "Physiology of biofilms"

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Duguid, Ian Gordon. Growth rate controlled studies on the physiology of Staphylococcus Epidermidis biofilms and their susceptibility to antibiotics. Univeristy of Manchester, 1993.

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Percival, Steven L. Microbiology of wounds. CRC Press, 2010.

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Makin, Stephen Anthony. Effect of the biofilm mode of growth on the physiology and surface chemistry of Escherichia Coli. University of Manchester, 1994.

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1933-, Quesnel Louis B., Gilbert P, and Handley Pauline S, eds. Microbial cell envelopes: Interactions and biofilms. Blackwell Scientific Publications, 1993.

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The Role of Biofilms in DeviceRelated Infections Springer Series on Biofilms. Springer, 2009.

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1928-, Fingerman Milton, Nagabhushanam Rachakonda, and Thompson Mary-Frances, eds. Biofilms, bioadhesion, corrosion, and biofouling. Science Publishers, 1999.

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(Editor), Jost Wingender, Thomas R. Neu (Editor), and Hans-Curt Flemming (Editor), eds. Microbial Extracellular Polymeric Substances: Characterization, Structure and Function. Springer, 1999.

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Microbial extracellular polymeric substances: Characterization, structure, and function. Springer, 1999.

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(Editor), Donald R. Demuth, and Richard Lamont (Editor), eds. Bacterial Cell-to-Cell Communication: Role in Virulence and Pathogenesis (Advances in Molecular and Cellular Microbiology). Cambridge University Press, 2006.

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R, Demuth Donald, and Lamont Richard J. 1961-, eds. Bacterial cell-to-cell communication: Role in virulence and pathogenesis. Cambridge University Press, 2006.

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Book chapters on the topic "Physiology of biofilms"

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Spormann, A. M. "Physiology of Microbes in Biofilms." In Current Topics in Microbiology and Immunology. Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/978-3-540-75418-3_2.

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Leadbeater, B. S. C., and Maureen E. Callow. "Formation, Composition and Physiology of Algal Biofilms." In Biofilms — Science and Technology. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-1824-8_15.

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Chávez de Paz, Luis E., and Philip D. Marsh. "Ecology and Physiology of Root Canal Microbial Biofilm Communities." In Springer Series on Biofilms. Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-47415-0_1.

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Lemos, José A., Jacqueline Abranches, Hyun Koo, Robert E. Marquis, and Robert A. Burne. "Protocols to Study the Physiology of Oral Biofilms." In Methods in Molecular Biology. Humana Press, 2010. http://dx.doi.org/10.1007/978-1-60761-820-1_7.

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Coquet, Laurent, Sébastien Vilain, Pascal Cosette, Thierry Jouenne, and Guy-Alain Junter. "A Proteomic Approach to Biofilm Cell Physiology." In Immobilization of Enzymes and Cells. Humana Press, 2006. http://dx.doi.org/10.1007/978-1-59745-053-9_35.

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Christensen, Bjarke Bak, Claus Sternberg, Jens Bo Andersen, et al. "[2] Molecular tools for study of biofilm physiology." In Biofilms. Elsevier, 1999. http://dx.doi.org/10.1016/s0076-6879(99)10004-1.

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Stickler, David J., Nicola S. Morris, and Carole Winters. "[35] Simple physical model to study formation and physiology of biofilms on urethral catheters." In Biofilms. Elsevier, 1999. http://dx.doi.org/10.1016/s0076-6879(99)10037-5.

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McLean, Robert J. C., Marvin Whiteley, Brian C. Hoskins, Paul D. Majors, and Mukul M. Sharma. "[20] Laboratory techniques for studying biofilm growth, physiology, and gene expression in flowing systems and porous media." In Biofilms. Elsevier, 1999. http://dx.doi.org/10.1016/s0076-6879(99)10022-3.

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Burne, Robert A., and Robert E. Marquis. "[28] Biofilm acid/base physiology and gene expression in oral bacteria." In Microbial Growth in Biofilms - Part B: Special Environments and Physicochemical Aspects. Elsevier, 2001. http://dx.doi.org/10.1016/s0076-6879(01)37029-5.

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Burne, Robert A., Robert G. Quivey, and Robert E. Marquis. "[33] Physiologic homeostasis and stress responses in oral biofilms." In Biofilms. Elsevier, 1999. http://dx.doi.org/10.1016/s0076-6879(99)10035-1.

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