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Dissertations / Theses on the topic 'Physiology of biofilms'

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1

Lethbridge, Samuel Aston. "Physiology and antibiotic susceptibility of mycobacterial biofilms." Thesis, St George's, University of London, 2018. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.754066.

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Treatment of tuberculosis requires months of antimycobacterial therapy. This tolerance to antibiotics displayed by Mycobacterium tuberculosis could be attributed to biofilm formation. Biofilms are the cause of many chronic infections. The aim of this thesis was to apply laboratory methods for the culture of Mycobacterium smegmatis and M. tuberculosis H37Rv biofilms and to further characterise these bacterial phenotypes in terms of their physiology, gene expression and drug susceptibility The Modified Robbins Device (MRD) and the Constant Depth Film Fermenter (CDFF) laboratory models were appli
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2

Gillan, David. "Morpho-physiology, genetics and ecological aspects of marine microbial biofilms :the study of the iron-encrusted biofilm associated with Montacuta ferruginosa (Mollusca, Bivalvia)." Doctoral thesis, Universite Libre de Bruxelles, 1999. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/211930.

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3

Hastings, Cody M. "Determination of the effects that a previously uncharacterized secreted product from Klebsiella pneumoniae has on Citrobacter freundii and Enterobacter cloacae biofilms." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/honors/419.

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More so than ever, Multiple Drug Resistant (MDR) bacteria are on the rise due to overuse of antibiotics along with natural selection for adaptations that enhance drug-resistant properties. One particular bacterial family, Enterobacteriaceae, has been problematic, exhibiting several bacterial members that have developed a precipitous resistance to modern antibiotics and are also primary causative agents of nosocomial, or hospital acquired, infections. Citrobacter freundii (CF) and Enterobacter cloacae (ECL) are two species of the Enterobacteriaceae family causing significant medical concern due
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4

Stark, Roger Matthew. "Studies on the growth and physiology of Helicobacter pylori." Thesis, University of the West of England, Bristol, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244291.

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5

Pfendler, Stéphane. "Intérêt du traitement par UV-C des communautés bactériennes, fongiques et des protistes autotrophes des biofilms colonisant la pierre patrimoniale : structure des peuplements, effets des UV-C sur la physiologie algale et innocuité du traitement vis à vis du support pictural." Thesis, Bourgogne Franche-Comté, 2017. http://www.theses.fr/2017UBFCD030/document.

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Dans le cadre de la conservation du patrimoine, les micro-organismes se développant sous forme de biofilm sont souvent considérés comme des agents entraînant des problèmes esthétiques et de détérioration du support colonisé. L’objectif général de cette thèse est l’utilisation de la lumière UV-C comme méthode de traitement des biofilms. Dans le but de comprendre la diversité des biofilms, les communautés bactériennes, fongiques et des protistes autotrophes ont été étudiées à l’aide d’une technique de séquençage haut débit. Puis, afin de comprendre les effets des UV-C sur les micro-algales (Chlo
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6

Gellé, Marie-Paule. "Physiologie des biofilms de streptocoques oraux : de la colonisation a la destructuration ; analyse microbiologique, physico-chimique et microscopique (doctorat : odontologie)." Reims, 1998. http://www.theses.fr/1998REIM0201.

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7

Khelissa, Simon Oussama. "Caractérisation des propriétés physiologiques associées aux cellules détachées de biofilms et étude des interactions aux interfaces entre bactéries et matériaux : cas de Staphylococcus aureus et Pseudomonas aeruginosa." Thesis, Lille 1, 2017. http://www.theses.fr/2017LIL10195.

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Le biofilm est à l’origine d’infections nosocomiales et d’intoxications alimentaires dans les secteurs alimentaire et hospitalier. Les bactéries structurées en biofilm peuvent se détacher et coloniser de nouvelles surfaces. Le risque microbiologique associé aux bactéries détachées de biofilm est peu étudié. Les travaux de thèse ont concerné, l’étude de l’effet des conditions de croissance sur les propriétés physicochimiques de surface de Staphylococcus aureus et Pseudomonas aeruginosa sous leurs formes détachées de biofilm et planctoniques, ainsi que leurs adhésion sur l'acier inoxydable (SS)
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8

Bzdrenga, Janek. "De la promiscuité des enzymes : cas des PLLs et de leur implication dans l'anti virulence bactérienne et la décontamination des organophosphorés." Thesis, Aix-Marseille, 2016. http://www.theses.fr/2016AIXM5018.

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Les PLLs sont une famille d’enzyme dont l’activité catalytique est double. L’activité lactonase permet entre autre de détruire les AHLs, molécules médiatrices de la communication chez les bactéries Gram négatives, empêchant ainsi la synchronisation à l’échelle de la population de la sécrétion de facteurs de virulence ou la formation de biofilm. Elles sont non seulement capables d’hydrolyser les molécules possédant un noyau lactone, mais aussi les phosphotriésters par promiscuité de substrat. L’activité phosphotriestérase permet de dégrader les composés organophosphorés (OPs) hautement toxiques
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9

Carvalhais, Virgínia Maria Dinis. "Effect of modulating S. Epidermidis biofilms dormancy in the biofilm physiology and host immune response." Tese, 2016. https://repositorio-aberto.up.pt/handle/10216/100331.

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10

Carvalhais, Virgínia Maria Dinis. "Effect of modulating S. Epidermidis biofilms dormancy in the biofilm physiology and host immune response." Doctoral thesis, 2016. https://repositorio-aberto.up.pt/handle/10216/100331.

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11

"Biofilm formation and regulation of biofilm-related genes in clinical isolates of Candida parapsilosis." 2014. http://repository.lib.cuhk.edu.hk/en/item/cuhk-1291545.

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The yeast Candida is an opportunistic human pathogen often associated with nosocomial infections. Understanding of the fungal virulence factor is limited. Adhesion and biofilm formation on prosthetic devices is likely to be one such factor for Candida infection. Biofilms form a structured community embedded by exopolymeric substances. Catheter associated Candidiasis is problematic since biofilm is hard to eradicate by antifungal therapy alone.<br>C. parapsilosis is a normal human commensal commonly isolated from human skin. C. parapsilosis is considered to be an emerging fungal pathogen and pa
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12

Sakhtah, Hassan. "Physiology of Pseudomonas Aeruginosa Phenazine Production and Transport." Thesis, 2016. https://doi.org/10.7916/D82B8XZV.

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Many bacteria secrete secondary metabolites, whose production is decoupled from active growth in laboratory cultures. Historically, the advantages of secondary metabolite production have mostly been explored in the context of cellular interactions, such as antibiotic effects on competing organisms, damage caused to host tissues during infection, or cell density-dependent signaling. However, recent studies in the opportunistic pathogen Pseudomonas aeruginosa have brought into focus the physiological effects of secondary metabolites on their producer and their implications for multicellular beha
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13

Cavaco, Gonçalo Francisco Cordeiro. "Peptidoglycan amidation of Staphylococcus aureus and bacteria cell physiology." Master's thesis, 2018. http://hdl.handle.net/10362/37048.

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Peptidoglycan is a highly dynamic macromolecule that undergoes several secondary modifications during its biosynthesis. The MurT-GatD enzymatic complex is necessary for the amidation of glutamate of the stem peptide of Staphylococcus aureus peptidoglycan. This secondary modification influences critical processes of S. aureus, such as growth rate, beta-lactam and lysozyme resistance. However, the mechanisms through which it influences S. aureus physiology remain unknown. In this study, several MRSA strains and respective murT-gatD mutants were used. Since peptidoglycan is a surface-expos
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14

Roubalová, Jana. "Optimalizace metodiky vedoucí k hodnocení citlivosti biofilm formujících mikrobiálních agens vůči látkám s antimikrobním potenciálem." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-380331.

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Charles University Faculty of Pharmacy in Hradec Králové Study program: Pharmacy Candidate: Jana Roubalová Consultant: RNDr. Klára Konečná, Ph.D. Title of thesis: Optimization of methods for sensitivity evaluation of biofilm-forming microbial agents towards substances with antimicrobial potential Background: The aim of this diploma thesis was to optimize the methodical approach to the production of yeast biofilms in vitro on the so-called pegs and the walls of the wells of the 96-well panel. This biofilm formation system should be an analogous approach to the commercially available Calgary Bio
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15

Redelman, Carly Virginia. "Antibiotic Treatment of Pseudomonas aeruginosa Biofilms Stimulates Expression of mgtE, a Virulence Modulator." 2012. http://hdl.handle.net/1805/2887.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>Pseudomonas aeruginosa is a gram negative opportunistic pathogen with the capacity to cause serious disease by forming biofilms, most notably in the lungs of cystic fibrosis (CF) patients. Biofilms are communities of microorganisms that adhere to a solid surface, undergo global regulatory changes, secrete exopolysaccharides, and are innately antibiotic resistant. Virulence modulation is an important tool utilized by P. aeruginosa to propagate infection and biofilm formation in the CF airway. Many different virulence modulatory pat
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16

Ribeiro, Ana Isabel Freitas. "Biofilmes, microbiomas e infeções da pele." Master's thesis, 2019. http://hdl.handle.net/10284/8698.

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A presente monografia tem como objetivo compreender os conceitos de biofilme e microbioma, e abordar algumas infeções da pele que poderão resultar do desequilíbrio do microbioma. Diferentes zonas da pele apresentam diferentes ecossistemas, sendo assim, habitadas por diferentes microrganismos. O microbioma representa a comunidade de microrganismos que naturalmente habitam no corpo humano, constituindo assim parte da identidade humana. Trata-se de um ecossistema complexo que reúne bactérias, fungos e vírus que vivem em comensalismo com o hospedeiro em diversos orgãos, incluindo na pele. A pele
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