Relevant bibliographies by topics / Phytophthora specific DNA probes

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  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Phytophthora specific DNA probes'

Author: Grafiati
Published: 2 June 2025
Last updated: 31 July 2025

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Journal articles on the topic "Phytophthora specific DNA probes"

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Bilodeau, Guillaume J., Frank N. Martin, Michael D. Coffey, and Cheryl L. Blomquist. "Development of a Multiplex Assay for Genus- and Species-Specific Detection of Phytophthora Based on Differences in Mitochondrial Gene Order." Phytopathology® 104, no. 7 (2014): 733–48. http://dx.doi.org/10.1094/phyto-09-13-0263-r.

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A molecular diagnostic assay for Phytophthora spp. that is specific, sensitive, has both genus- and species-specific detection capabilities multiplexed, and can be used to systematically develop markers for detection of a wide range of species would facilitate research and regulatory efforts. To address this need, a marker system was developed based on the high copy sequences of the mitochondrial DNA utilizing gene orders that were highly conserved in the genus Phytophthora but different in the related genus Pythium and plants to reduce the importance of highly controlled annealing temperature
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Sikora, Katarzyna, Els Verstappen, Odette Mendes, Cor Schoen, Jean Ristaino, and Peter Bonants. "A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes." Phytopathology® 102, no. 6 (2012): 635–45. http://dx.doi.org/10.1094/phyto-11-11-0309.

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The genus Phytophthora consists of many species that cause important diseases in ornamental, agronomic, and forest ecosystems worldwide. Molecular methods have been developed for detection and identification of one or several species of Phytophthora in single or multiplex reactions. In this article, we describe a padlock probe (PLP)-based multiplex method of detection and identification for many Phytophthora spp. simultaneously. A generic TaqMan polymerase chain reaction assay, which detects all known Phytophthora spp., is conducted first, followed by a species-specific PLP ligation. A 96-well
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Miles, Timothy D., Frank N. Martin, Gregg P. Robideau, Guillaume J. Bilodeau, and Michael D. Coffey. "Systematic Development of Phytophthora Species-Specific Mitochondrial Diagnostic Markers for Economically Important Members of the Genus." Plant Disease 101, no. 7 (2017): 1162–70. http://dx.doi.org/10.1094/pdis-09-16-1224-re.

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The genus Phytophthora contains many invasive species to the U.S.A. that have the potential to cause significant damage to agriculture and native ecosystems. A genus and species-specific diagnostic assay was previously reported based on mitochondrial gene order differences that allowed for the systematic development of 14 species-specific TaqMan probes for pathogen detection ( Bilodeau et al. 2014 ). In this study, an additional 32 species-specific TaqMan probes for detection of primarily invasive species have been validated against 145 Phytophthora taxa as well as a range of Pythium and plant
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Vitas, Adomas, Tomasz Oszako, Justyna A. Nowakowska, Katarzyna Sikora, and Antanina Stankevičienėm. "First records of Phytophthora spp. based on DNA analysis in Lithuania." Folia Forestalia Polonica, Series A - Forestry 54(1) (March 1, 2012): 25–31. https://doi.org/10.5281/zenodo.30881.

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The assessment of alien invasive species of Phytophthora genus causing serious forest tree species diseases was carried out in Lithuania. The presence of Phytophthora DNA was recorded for the first time using real-time PCR analysis on 23 DNA samples. The sampling included wood from diseased trees, leaves from shrubs, leaves baited in water, and soil samples taken around diseased plants. Extracted DNA from soil and plant tissues was tested for the presence of Phytophthora. All analysed samples were positively recognized by Phytophthora-specific probe during real-time PCR which proved the presen
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McCoy, Austin G., Timothy D. Miles, Guillaume J. Bilodeau, et al. "Validation of a Preformulated, Field Deployable, Recombinase Polymerase Amplification Assay for Phytophthora Species." Plants 9, no. 4 (2020): 466. http://dx.doi.org/10.3390/plants9040466.

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Recombinase polymerase amplification (RPA) assays are valuable molecular diagnostic tools that can detect and identify plant pathogens in the field without time-consuming DNA extractions. Historically, RPA assay reagents were commercially available as a lyophilized pellet in microfuge strip tubes, but have become available in liquid form more recently—both require the addition of primers and probes prior to use, which can be challenging to handle in a field setting. Lyophilization of primers and probes, along with RPA reagents, contained within a single tube limits the risk of contamination, e
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Tooley, Paul W., Frank N. Martin, Marie M. Carras, and Reid D. Frederick. "Real-Time Fluorescent Polymerase Chain Reaction Detection of Phytophthora ramorum and Phytophthora pseudosyringae Using Mitochondrial Gene Regions." Phytopathology® 96, no. 4 (2006): 336–45. http://dx.doi.org/10.1094/phyto-96-0336.

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A real-time fluorescent polymerase chain reaction (PCR) detection method for the sudden oak death pathogen Phytophthora ramorum was developed based on mitochondrial DNA sequence with an ABI Prism 7700 (TaqMan) Sequence Detection System. Primers and probes were also developed for detecting P. pseudosyringae, a newly described species that causes symptoms similar to P. ramorum on certain hosts. The species-specific primer-probe systems were combined in a multiplex assay with a plant primer-probe system to allow plant DNA present in extracted samples to serve as a positive control in each reactio
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Whisson, SC, BJ Howlett, ECY Liew, DJ Maclean, JM Manners, and JAG Irwin. "An Assessment of Genetic Relationships between Members of the Phytophthora megasperma Complex and Phytophthora vignae using Molecular Markers." Australian Systematic Botany 6, no. 4 (1993): 295. http://dx.doi.org/10.1071/sb9930295.

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Genetic relationships between Phytophora megasperma f. sp. glycinea (Pmg) and morphologically similar taxa, P. megasperma f. sp. medicaginis (Pmm), P. megasperma f. sp. trifolii (Pmt), P. megasperma from Douglas Fir (PmDF) and asparagus (PmAS) and Phytophthora vignae, were explored by restriction fragment length polymorphism (RFLP) analysis of nuclear DNA using random genomic multi-copy, cDNA, and ribosomal DNA probes as well as random amplified polymorphic DNA (RAPDs) and RFLP analysis of ribosomal intergenic spacer regions amplified by the polymerase chain reaction (PCR). Each method detecte
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Lievens, B., I. R. M. Hanssen, A. C. R. C. Vanachter, B. P. A. Cammue, and B. P. H. J. Thomma. "Root and Foot Rot on Tomato Caused by Phytophthora infestans Detected in Belgium." Plant Disease 88, no. 1 (2004): 86. http://dx.doi.org/10.1094/pdis.2004.88.1.86a.

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In January 2003, a severe root and foot rot was observed on 2-month-old wilted tomato (Lycopersicon esculentum Mill.) plants in a large-scale (2.5 ha) commercial greenhouse setting in Belgium. Tomato plants (10%) produced from healthy nursery-grown seedlings and planted to new, clean rockwool and drip irrigation with UV-disinfected water developed symptoms. Symptom development was restricted to lower plant parts with severe rotting of the entire root system and dark lesions girdling the stem base. No symptoms of disease were observed on the foliage or upper stems. Cross sections of the stem ba
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Oszako, Tomasz, Katarzyna Anna Kubiak, Marta Siebyła, and Justyna Anna Nowakowska. "Slow Sand Filters as a part of integrated protection of seedlings against disease in forest nurseries." Forest Research Papers 74 (1) (March 1, 2013): 49–56. https://doi.org/10.2478/frp-2013-0006.

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Slow Sand Filters (SSF) are a biological method used to protect nursery plants, from pathogen infections which can cause serious diseases in many forest tree species. Thanks to SSF application the number of phytopathogens in nurseries can be significantly reduced, as demonstrated by many field and greenhouse experiments (e.g. in Polish nurseries, and for horticultural crops in Germany and The Netherlands). In this study, the effect of pollution from fertilizers and fungicides used in agriculture (e.g. PCNB) on the efficiency of SSFs was assessed. A quantitative analysis was performed of the co
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Goodwin, P. H., B. C. Kirkpatrick, and J. M. Duniway. "Identification of Phytophthora citrophthora with Cloned DNA Probes." Applied and Environmental Microbiology 56, no. 3 (1990): 669–74. http://dx.doi.org/10.1128/aem.56.3.669-674.1990.

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Dissertations / Theses on the topic "Phytophthora specific DNA probes"

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Emanuel, Margot. "Species-specific DNA probes for the identification of Actinobacillus actinomycetemcomitans." Master's thesis, University of Cape Town, 1990. http://hdl.handle.net/11427/24965.

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A DNA probe was developed for the identification of the periodontal pathogen, Actinobacillus actinomycetemcomitans. Chromosomal DNA was extracted from A. actinomycetemcomitans, digested with a restriction enzyme, Sau3A, ligated to plasmid DNA (pUC18) and transformed into JM109 cells to give a partial A. actinomycetemcomitans library. The library was screened using Southern blot analysis. Out of the nine inserts tested, one was found to be species specific as it did not cross-hybridise to Haemophilus aphrophilus, a closely related organism which occurs in the normal oral microflora, nor did it
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Matsumoto, Katsuhiko. "Development of fluorescent probes for sequence-specific detection of DNA and RNA." Kyoto University, 2012. http://hdl.handle.net/2433/157395.

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Clapp, Justin Peter. "Selective enrichment of genomic DNA data for the isolation of species-specific probes in insects." Thesis, University of Hertfordshire, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296538.

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Brind'Amour, Julie. "Flow cytometry analysis and sorting of chromosomes following hybridization with fluorescent probes that target specific DNA repeat sequences." Thesis, University of British Columbia, 2011. http://hdl.handle.net/2429/35973.

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Traditional cytogenetic approaches allow analysis of the chromosomal composition (karyotype) of mitotic cells fixed on slides cells by microscopy. The combination of karyotyping and Fluorescence In Situ Hybridization (FISH) enables the detection of specific target sequences on individual chromosomes. Disadvantages are that traditional cytogenetic approaches are very labor and time consuming and that chromosome specific information from only a few dozen cells has poor statistical power. An alternative is flow karyotyping, a method to analyze chromosomes in suspension by flow cytometry. For flow
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Berriatua, Eduardo. "An epidemiological study of ovine coccidiosis and the development of specific DNA probes for Eimeria crandallis and Eimeria ovinoidalis." Thesis, University of Bristol, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385475.

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Rothe, Jessica. "Establishment of a Y-chromosome specific extraction method for the separation of Y-chromosomal haplotypes from male DNA mixtures." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2014. http://dx.doi.org/10.18452/16995.

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Die Haplotypspezifische Extraktion (HSE) bietet für die Analyse von männlichen Mischprofilen einen neuen und direkteren Lösungsansatz, in dem die haploiden Y-chromosomalen DNS-Komponenten der einzelnen Individuen bereits vor der Analyse der individual spezifischen Marker separiert werden können und dadurch eine wirkliche physische Trennung erreicht wird. Die HSE verwendet Y-chromosomalen SNPs für die Erstellung allelspezifische Extraktionssonden, die nun gezielt nur die Marker der extrahierten DNS Komponente bzw. einer Person separieren sollen. Dabei werden im Hybridisationsschritt der HSE se
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卜樂妙. "Selection and Application of Species-specific DNA Probes for Plant Pathogenic Pythium." Thesis, 1994. http://ndltd.ncl.edu.tw/handle/82360286226078502966.

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碩士<br>東吳大學<br>微生物學研究所<br>82<br>The genus Pythium is one of the most important plant pathogenic fungi. Traditionally, species identification in Pythium is based upon morphological features including charactertistics of asexual reproduction and sexual reproduction. However, many species of Pythium possess similar morphological features and some even failed to produce reproductive structures, hence resulting in the difficulty in their identification. In this study, molecular technology was used to facilitate the identification and detection of different Pythium species. Mitochondrial DNAs of
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WANG, SHU-ZHENG, and 王淑珍. "Cloning of the Salmonella specific DNA probes, preparation of oligonucleotide probes and their application in detection of Salmonella in foods." Thesis, 1991. http://ndltd.ncl.edu.tw/handle/53579813271114731227.

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Spierings, Aldegonda Maria Johanna. "Enterobacterial species-specific DNA probes based on the gene for outer membrane proteine PhoE = Enterobacteriële species-specifieke DNA probes gebaseerd op het gen coderend voor het buitenmembraaneiwit PhoE /." 1992. http://www.gbv.de/dms/bs/toc/131131567.pdf.

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Poet, Steven E. "Development and diagnostic applications of a group-specific caliciviridae cDNA hybridization probe cloned from San Miguel sea lion virus, type 5, a calicivirus of ocean origin." Thesis, 1994. http://hdl.handle.net/1957/35569.

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Books on the topic "Phytophthora specific DNA probes"

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Metcalfe, Mark Andrew. Development of a solution-hybridization assay for eubacterial 16S ribosomal RNA, employing oligomers of DNA or 2'-O-allyl RNA as sequence-specific probes. University of Birmingham, 1995.

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H, Persing David, ed. Diagnostic molecular microbiology: Principles and applications. American Society for Microbiology, 1993.

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Poet, Steven E. Development and diagnostic applications of a group-specific caliciviridae cDNA hybridization probe cloned from San Miguel sea lion virus, type 5, a calicivirus of ocean origin. 1994.

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Persing, David H., Thomas F. Smith, Fred C. Tenover, and Thomas J. White. Diagnostic Molecular Microbiology: Principles and Applications. American Society Microbiology, 1993.

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Book chapters on the topic "Phytophthora specific DNA probes"

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Somers, D. J., G. Séguin-Swartz, G. Demmon, and J. Danielson. "Genome Specific DNA Probes in Crucifers." In Stadler Genetics Symposia Series. Springer US, 2000. http://dx.doi.org/10.1007/978-1-4615-4235-3_26.

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Bertucci, Alessandro, Alex Manicardi, and Roberto Corradini. "Advanced Molecular Probes for Sequence-Specific DNA Recognition." In Detection of Non-Amplified Genomic DNA. Springer Netherlands, 2012. http://dx.doi.org/10.1007/978-94-007-1226-3_4.

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Cooper, J. E., and A. J. Bjourson. "Simplified Subtraction-Hybridization System for Isolation Of Strain-Specific Rhizobium DNA Probes." In The Release of Genetically Modified Microorganisms—REGEM 2. Springer US, 1992. http://dx.doi.org/10.1007/978-1-4613-0493-7_33.

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Crampton, Julian M., and Susannah M. Hill. "Generation and use of species-specific DNA probes for insect vector identification." In The Molecular Biology of Insect Disease Vectors. Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-009-1535-0_33.

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Kawamoto, Yusuke. "Introduction: Sequence-Specific DNA Binding Pyrrole–Imidazole Polyamides and Their Applications." In Synthesis and Biological Evaluation of Pyrrole–Imidazole Polyamide Probes for Visualization of Telomeres. Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-6912-4_1.

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Burghoff, Robert L., Jens Beator, and Michael A. Harvey. "Psoralen Biotin: A Novel Reagent for Non-Enzymatic and Specific Labeling of Nucleic Acid Probes and Oligonucleotides." In Modern Applications of DNA Amplification Techniques. Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-5379-3_8.

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Epplen, Jörg T., and Judith MÁthÉ. "Multilocus DNA Fingerprinting Using Nonradioactively Labeled Oligonucleotide Probes Specific for Simple Repeat Elements." In Nonradioactive Analysis of Biomolecules. Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57206-7_40.

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Edward, Roy. "Applications and Caveats on the Utilization of DNA-Specific Probes in Cell-Based Assays." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-7357-6_1.

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Pulleyblank, David E., Mark Glover, Chuck Farah, and David B. Haniford. "The Specificity of “Single Strand Specific Endonucleases”: Probes of phosphodiester conformation in double stranded nucleic acids. Left-Handed Polypurine/polypyrimidine structures. Long range transmission of conformational information in DNA." In Unusual DNA Structures. Springer New York, 1988. http://dx.doi.org/10.1007/978-1-4612-3800-3_2.

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Signer, E. N., and A. J. Jeffreys. "Application of human minisatellite probes to the development of informative DNA fingerprints and the isolation of locus-specific markers in animals." In DNA Fingerprinting: State of the Science. Birkhäuser Basel, 1993. http://dx.doi.org/10.1007/978-3-0348-8583-6_42.

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Conference papers on the topic "Phytophthora specific DNA probes"

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Zhang, Jiacheng, George Alexandrou, Chris Toumazou, and Melpomeni Kalofonou. "Automating the Design of Cancer Specific DNA Probes Using Computational Algorithms." In 2021 43rd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC). IEEE, 2021. http://dx.doi.org/10.1109/embc46164.2021.9630589.

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Zhou, H., K. Ma, G. Jia, J. Zoval, and M. Madou. "Micro Contact Printing of DNA Molecules." In ASME 2004 3rd Integrated Nanosystems Conference. ASMEDC, 2004. http://dx.doi.org/10.1115/nano2004-46060.

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The development of DNA sensors has attracted substantial research efforts. Such devices could be used for the rapid identification of pathogens in humans, animals, and plant; in the detection of specific genes in animal and plant breeding; and in the diagnosis of human genetic disorders. The first step to fabricate the DNA sensors is the probe immobilization on the suitable substrate. Traditionally, the DNA probes are spotted on the substrate while the technique hardly controlled the small pattern and surface density of DNA probes. The main challenge here is to achieve probe layer uniformity a
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de la Salle, C., M. J. Baas, L. Grunebaum, R. Gialeraki, T. Mandalaki, and J.-P. Cazenave. "MOLECULAR ANALYSIS OF COAGULATION FACTOR VIII AND IX GENES BY DNA PROBES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643873.

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About 250 individuals belonging to 44 families with hemophilia A or B were studied in our laboratory. The detection of carriers was first established by pedigree analysis of each family . and coagulation and immunological assays of factor VIII or IX. The availability of specific probes for the molecular study of these two genes makes possible a diagnosis with certainty in the case of informative families. 25 families of hemophilia A were studied. For each person, blood was collected into EDTA and leucocyte DNA was extracted, digested by restriction endonucleases, electrophoresed in 0.9 % agaro
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Bushaev, A. A., M. Ateiah, M. S. Rubel, and D. M. Kolpashchikov. "DEVELOPMENT OF FLUORESCENT HYBRIDIZATION PROBES FOR HIGHLY SELECTIVE DETECTION OF DOUBLE STRANDED DNA ANALYTES AT 37 °C." In X Международная конференция молодых ученых: биоинформатиков, биотехнологов, биофизиков, вирусологов и молекулярных биологов — 2023. Novosibirsk State University, 2023. http://dx.doi.org/10.25205/978-5-4437-1526-1-273.

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A DNA nanomachine is an association of a discrete number of DNA strands that undergoes structural rearrangements in response to the presence of a specific analyte [1]. DNA nanomachines have proven to be a sensitive and selective diagnostic method [2]. A disadvantage that hinders their practical use is that existing machines do not work under physiological conditions. The introduction of artificial locked nucleic acids (LNAs) into their structure can help increase the sensitivity of nanomachines at this temperature.
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Inoue, Shinnosuke, Woon-Hong Yeo, Jong-Hoon Kim, et al. "Amplification-Free DNA Detection Using a Microtip-Sensor Decorated With LNA Probes for Rapid TB Screening." In ASME 2011 International Mechanical Engineering Congress and Exposition. ASMEDC, 2011. http://dx.doi.org/10.1115/imece2011-64378.

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Tuberculosis (TB) is an epidemic affecting one-third of the world’s population, mostly in developing and low-resource settings. People having active pulmonary TB are considered highly infectious; therefore, it is critical to identify and treat these patients rapidly before spreading to others. However, the most reliable TB diagnostic methods of bacterial culture or nucleic acid amplification are time-consuming and expensive. The challenge of TB diagnosis lies in highly sensitive and specific screening with low cost. Here, we present an LNA-modified microtip-sensor, which is capable of selectiv
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Bosma, P. J., E. A. van den Berg, and T. Kooistra. "ISOLATION OF THE GENE CODING FOR HUMAN PLASMINOGEN ACTIVATOR INHIBITOR TYPE 1 (PAI-1)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644440.

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A human placenta genomic DNA cosmid library was screened for the presence of the PAI-1 gene using a cDNA probe coding for PAI-1. Two overlapping recombinant cosmids were obtained that contain human DNA spanning 55 kb. The cosmids were mapped using 3' and 5' end probes isolated from an almost full-length cDNA clone of 2.5 kb. The two cosmids were found to contain the entire structural PAI-1 gene (approximately 15 kb) and also included 25 kb 5' flanking sequences. The transcription initiation site was identified by SI nuclease protection experiments and the promotor region was sequenced. Further
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Matthews, R. J., I. R. Peake, and A. L. Bloom. "POINT-MUTATION OF FACTOR VIII CODING SEQUENCES IN HAEMOPHILIA A." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644013.

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In order to study the molecular basis of haemophilia A, DNA from 26 haemophilia A patients (8 severe with inhibitors, 13 severe noninhibitors and 5 mild/moderate) was screened by the Southern blotting method with FVIII cDNA probe A (a i.7kb Kpnl cDNA fragment that spans exons 1 to 12) probe B (a 4.7kb EcoRI cDNA fragment that contains exons 14 to 25 and part of exon 26) probe C (a 1.8kb EcoRI cDNA fragment that contains the remainder of exon 26 and probe D (an Apal/EcoRI 783bp cDNA fragment that includes all of exons 22 to 25 and parts of exons 21 and 26). All cDNA probes were kindly provided
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Glišović, Ana, Tijana Relić, and Nikola Mihajlović. "HPV genotyping at the City Institute for Public Health Belgrade from January 2023 to July 2024." In Proceedings of the International Congress Public Health - Achievements and Challenges. Institute of Public Health of Serbia "Dr Milan Jovanović Batut", 2024. http://dx.doi.org/10.5937/batutphco24122g.

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Introduction: Human papillomavirus (HPV) infection is an indispensable factor in the carcinogenesis of the cervical epithelium. Persistent infection with high-risk HPV genotypes poses a particular risk. According to the World Health Organization (WHO) guidelines, molecular biological assays that detect the presence of HPV DNA and determine the viral genotype in cervical samples are diagnostically superior for early infection detection compared to conventional cytological Pap smears. Objectives and Methods: To present the results of HPV genotyping from endocervical swab samples at the City Inst
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Sadler, J. Evan. "THE MOLECULAR BIOLOGY OF VON WILLEBRAND FACTOR." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643930.

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Human von Willebrand factor (vWF) is a plasma glycoprotein that is synthesized by endothelial cells and megakaryocytes, and perhaps by syncytiotrophoblast of placenta. The biosynthesis of vWF is very complex, involving proteolytic processing, glycosyla-tion, disulfide bond formation, and sulfation. Mature vWF consists of a single subunit of ∼ 250,000 daltons that is assembled into multimer ranging from dimers to species of over 10 million daltons. vWF performs its essential hemostatic function through several binding interactions, forming a bridge between specific receptors on the platelet sur
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Reports on the topic "Phytophthora specific DNA probes"

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Tumwasorn, Somying, Ajcharaporn Sawatpanich, Nibondh Udomsantisuk, and Kamon Kawkitinarong. Development of test kit for detection of rifampin resistance in mycobacterium tuberculosis by PCR-reverse line blot hybridization. Faculty of Medicine, Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.25.

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A genetic test by PCR-reverse line blot hybridization was developed for rapid detection of mycobacterium tuberculosis and rifampin resistance simultaneously. Duplex PCR targeting IS 6110 and rpoB gene was employed to detect M. tuberculosis and the rpoB fragment with rifampin resistance hot spot region. The analytical sensitivity of duplex PCR for IS 6110 and rpoB gene was found to be 10 and 100fg of M.Tuberculosis H37Rv DNA, respectively. Since duplex PCR was specific to M. tuberculosis complex, it was thus able to be applied directly in clinical specimens. Oligonucleotide probes were designed
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Jordan, Ramon L., Abed Gera, Hei-Ti Hsu, Andre Franck, and Gad Loebenstein. Detection and Diagnosis of Virus Diseases of Pelargonium. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568793.bard.

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Pelargonium (Geranium) is the number one pot plant in many areas of the United States and Europe. Israel and the U.S. send to Europe rooted cuttings, foundation stocks and finished plants to supply a certain share of the market. Geraniums are propagated mainly vegetatively from cuttings. Consequently, viral diseases have been and remain a major threat to the production and quality of the crop. Among the viruses isolated from naturally infected geraniums, 11 are not specific to Pelargonium and occur in other crops while 6 other viruses seem to be limited to geranium. However, several of these v
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3

Sink, Ken, Shamay Izhar, and Abraham Nachmias. Asymmetric Somatic Hybridization: Developing a Gene Transfer System for Solanaceous Vegetable Crops. United States Department of Agriculture, 1996. http://dx.doi.org/10.32747/1996.7613010.bard.

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Highly asymmetric somatic hybrid plants were obtained by PEG/DMSO fusion of gamma irradiated (100, 250, 7500 and 1000 Gy) protoplasts of a (KmR-) interspecific hybrid Lycopersicon esculentum x L. pennellii (EP) with protoplasts of eggplant (E). Somatic hybrid calli were selected based on kanamycin resistance and verified by PCR of the NptII gene, RAPD's and Southern's using potato rDNA pTHG2 probes. Flow cytometry indicated all hybrid calli that did not regenerate shoots were 5-9n. Three asymmetric plants regenerated only from callus close to 4n and such calli oly occurred when EP received 100
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Levisohn, Sharon, Mark Jackwood, and Stanley Kleven. New Approaches for Detection of Mycoplasma iowae Infection in Turkeys. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7612834.bard.

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Mycoplasma iowae (Mi) is a pathogenic avian mycoplasma which causes mortality in turkey embryos and as such has clinical and economic significance for the turkey breeder industry. Control of Mi infection is severely hampered by lack of adequate diagnostic tests, together with resistance to most antibiotics and resilience to environment. A markedly high degree of intra-species antigenic variation also contributes to difficulties in detection and control of infection. In this project we have designed an innovative gene-based diagnostic test based on specific amplification of the 16S rRNA gene of
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5

Epel, Bernard, and Roger Beachy. Mechanisms of intra- and intercellular targeting and movement of tobacco mosaic virus. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7695874.bard.

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To cause disease, plant viruses must replicate and spread locally and systemically within the host. Cell-to-cell virus spread is mediated by virus-encoded movement proteins (MPs), which modify the structure and function of plasmodesmata (Pd), trans-wall co-axial membranous tunnels that interconnect the cytoplasm of neighboring cells. Tobacco mosaic virus (TMV) employ a single MP for cell- cell spread and for which CP is not required. The PIs, Beachy (USA) and Epel (Israel) and co-workers, developed new tools and approaches for study of the mechanism of spread of TMV that lead to a partial iden
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6

Funkenstein, Bruria, and Cunming Duan. GH-IGF Axis in Sparus aurata: Possible Applications to Genetic Selection. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7580665.bard.

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Many factors affect growth rate in fish: environmental, nutritional, genetics and endogenous (physiological) factors. Endogenous control of growth is very complex and many hormone systems are involved. Nevertheless, it is well accepted that growth hormone (GH) plays a major role in stimulating somatic growth. Although it is now clear that most, if not all, components of the GH-IGF axis exist in fish, we are still far from understanding how fish grow. In our project we used as the experimental system a marine fish, the gilthead sea bream (Sparus aurata), which inhabits lagoons along the Mediter
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