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Dissertations / Theses on the topic 'Pichia pastoria'

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Published: 4 June 2021
Last updated: 1 February 2022

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1

Johnson, Monique A. "Novel genetic selections for peroxisome biogenesis mutants (pex) and the isolation and characterization of PEX14 and Pex14p in Pichia Pastoris /." Full text open access at:, 2000. http://content.ohsu.edu/u?/etd,15.

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2

Chaharmahali, Pegah M. "Calcium homeostasis in Pichia pastoris." Scholarly Commons, 2014. https://scholarlycommons.pacific.edu/uop_etds/179.

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Pichia pastoris is a methylotrophic yeast that has been used widely in biological and industrial researches. P. pastoris is able to produce from milligram to gram quantities of protein. In this study, we examined the effects of calcium and magnesium on growth, heterologous protein expression, and calcium homeostasis in P. pastoris . The divalent cations calcium and magnesium are responsible for diverse roles in in the function of the cells in eukaryotes. Although it is known that calcium is responsible for many biological functions in eukaryotes, there is a limited understanding of the calcium
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3

Boudreau, Vanessa. "Production inductible d'anhydrase carbonique par pichia pastoris." Thesis, Université Laval, 2011. http://www.theses.ulaval.ca/2011/28711/28711.pdf.

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4

Felix, Wagner Pereira. "ProduÃÃo heterÃloga de frutalina em Pichia pastoris." Universidade Federal do CearÃ, 2008. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=2314.

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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico<br>A frutalina, lectina &#945;-D-galactose ligante, foi expressa e processada num sistema heterÃlogo para expressÃo de proteÃnas utilizando a levedura metilotrÃfica Pichia pastoris. Para atingir os objetivos esperados foram desenvolvidas trÃs estratÃgias: identificar o gene que codifica para a frutalina a partir do DNA genÃmico obtido de folhas de A. incisa; sintetizar, por uma empresa especializada, o gene que codifica para as cadeias polipeptÃdicas da frutalina, otimizando-o para a expressÃo em P. pastoris e isolar o gene que codi
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5

Aizemberg, Raquel [UNESP]. "Produção de glicerol quinase em Pichia pastoris." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/88349.

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Made available in DSpace on 2014-06-11T19:23:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-08-12Bitstream added on 2014-06-13T18:09:34Z : No. of bitstreams: 1 aizemberg_r_me_arafcf.pdf: 685654 bytes, checksum: d058342c2d66649275266dc34dcb968d (MD5)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>Universidade Estadual Paulista (UNESP)<br>A levedura Pichia pastoris vem sendo largamente utilizada como um eficiente sistema de expressão para a produção de proteínas heterólogas, pois é um sistema seguro, fácil e mais barato que sistemas de expressão de outros eucari
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6

Aizemberg, Raquel. "Produção de glicerol quinase em Pichia pastoris /." Araraquara : [s.n.], 2011. http://hdl.handle.net/11449/88349.

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Orientador: Edwil Aparecida de Lucca Gattás<br>Banca: Eleonora Cano Carmona<br>Banca: Rubens Monti<br>Resumo: A levedura Pichia pastoris vem sendo largamente utilizada como um eficiente sistema de expressão para a produção de proteínas heterólogas, pois é um sistema seguro, fácil e mais barato que sistemas de expressão de outros eucariotos. Neste trabalho, a enzima de interesse é a glicerol quinase (GK), que cataliza a transferência do fosfato terminal do ATP para o glicerol originando glicerol-3-fosfato e ADP. Esta reação pode ser utilizada na determinação da concentração de glicerol, subprod
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7

Felix, Wagner Pereira. "Produção heteróloga de frutalina em Pichia pastoris." reponame:Repositório Institucional da UFC, 2008. http://www.repositorio.ufc.br/handle/riufc/18810.

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FELIX, Wagner Pereira. Produção heteróloga de frutalina em Pichia pastoris. 2008. 113 f. Tese (Doutorado em bioquímica)- Universidade Federal do Ceará, Fortaleza-CE, 2008.<br>Submitted by Elineudson Ribeiro (elineudsonr@gmail.com) on 2016-07-28T15:13:10Z No. of bitstreams: 1 2008_tese_wpfelix.pdf: 3013919 bytes, checksum: 5ad2f9b358ba8740f49b54a25064ab73 (MD5)<br>Approved for entry into archive by José Jairo Viana de Sousa (jairo@ufc.br) on 2016-08-02T14:44:01Z (GMT) No. of bitstreams: 1 2008_tese_wpfelix.pdf: 3013919 bytes, checksum: 5ad2f9b358ba8740f49b54a25064ab73 (MD5)<br>Made available in
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8

Böttner, Mewes. "Die Expression humaner Proteine in der Hefe Pichia pastoris: Hochdurchsatzverfahren und bioinformatische Identifizierung von Expression-beeinflussenden Sequenzmerkmalen." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972858717.

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9

Gunduz, Burcu. "Recombinant Transglutaminase Production By Metabolically Engineered Pichia Pastoris." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614761/index.pdf.

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Transglutaminases (EC 2.3.2.13) are enzymes that catalyze an acyl transfer reaction between a &gamma<br>-carboxyamide group of a peptide bound glutaminyl residue (acyl donor) and a variety of primary amines (acyl acceptors), including the amino group lysine. Transglutaminase has a potential in obtaining proteins with novel properties, improving nutritional quality of foods with the addition of essential amino acids, preparing heat stable gels, developing rheological properties and mechanical strength of foods and reducing the applications of food additives. The aim of this study is to develop
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10

Fonseca, Marisa Cristina da. "Produção de estreptavidina recombinante pela levedura Pichia pastoris." Universidade Federal de Viçosa, 2006. http://locus.ufv.br/handle/123456789/5386.

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Made available in DSpace on 2015-03-26T13:52:02Z (GMT). No. of bitstreams: 1 texto completo.pdf: 1210492 bytes, checksum: 9b50652b8d6d51ccdb4063535bd8ec12 (MD5) Previous issue date: 2006-02-20<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Streptavidin has been exploited as affinity tag to isolate protein in biotinilated columns. Recombinant Pichia pastoris KM71/Stp strains containing the streptavidin core gene were cultured in fed-batch generating 150 g L-1 biomass. This biomass was achieved with a simpler and shorter process that has never been reported. The yeast was pr
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11

Thor, Der. "Cloning and characterization of MET2 in Pichia pastoris." Scholarly Commons, 2002. https://scholarlycommons.pacific.edu/uop_etds/570.

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The methylotrophic yeast, Pichia pastoris, has been used as a protein expression system to express over 500 heterologous proteins. P. pastoris provides many advantages over other organisms that have been utilized for this purpose. In this project, we developed a new host/selectable marker and auxotrophic strains of P. pastoris based on methionine biosynthesis to increase P. pastoris's versatility as a host for homologous protein expression. This was accomplished by selecting for a yeast that is deficient in methionine biosynthesis, P. pastoris (yJC239), and gene complementation through transfo
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12

Aw, Rochelle. "Factors affecting the specific productivity of Pichia pastoris." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/11047.

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Pichia pastoris has become one of the most popular recombinant protein expression platforms, despite the lack of understanding into the fundamentals of protein expression. Whilst P. pastoris exhibits high volumetric productivity, it has a low specific productivity, which can be further reduced by protein-specific problems. This thesis employs several strategies commonly used to increase specific productivity, and assesses their impact on the productivity and cell biology of P. pastoris. Gene dosage has been reported to increase titre; therefore multiple copies of human serum albumin were integ
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13

Araújo, Diana Filipa Vieira. "Production of chitin-glucan complex by Pichia pastoris." Master's thesis, Faculdade de Ciencias e Tecnologia, 2013. http://hdl.handle.net/10362/10897.

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Dissertation for the Degree of Master in Biotechnology<br>The yeast Pichia pastoris produces chitin-glucan complex (CGC), as a cell wall component. CGC is composed of two types of biopolymers, chitin and β-glucans, which confer it great potential for use in the food, cosmetic and pharmaceutical industries. CGC hydrolysis, allows obtaining chitin/chitosan and glucans individually. The chitin and chitosan obtained from CGC have the considerable advantage of being of non-animal origin, which further extends their applications. In last year’s, the production of CGC by Pichia pastoris was realized
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14

Rampasio, Raquel Rodrigues 1986. "Estudos sobre a clonagem e expressão do gene SEH1 (epóxido hidrolase) de Pichia stipitis EM Pichia pastoris." [s.n.], 2012. http://repositorio.unicamp.br/jspui/handle/REPOSIP/248442.

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Orientador: Luciana Gonzaga de Oliveira<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Química<br>Made available in DSpace on 2018-08-22T05:28:22Z (GMT). No. of bitstreams: 1 Rampasio_RaquelRodrigues_M.pdf: 2052024 bytes, checksum: 07f4cd2fcba87af264e6efceab06d527 (MD5) Previous issue date: 2012<br>Resumo: Epóxidos enantiopuros e dióis vicinais têm sido utilizados na síntese de inúeras moléculas bioativas. Dessa forma, as epóxido-hidrolases microbianas capazes de hidrolisar enantioseletivamente epóxidos racêmicos emergiram como uma alternativa promissora na obten
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15

Grove, Heather Lee. "Cloning and characterization of the Pichia Pastoris PMR1 gene." Scholarly Commons, 2005. https://scholarlycommons.pacific.edu/uop_etds/613.

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Pichia pastoris, a popular protein expression system, is limited in its ability to secrete heterologous proteins. The PMR1 gene, the disruption of which is known to improve the secretion of prochymosin, human prourokinase, and human tissue plasminogen activator in Saccharomyces cerevisiae, was cloned from P. pastoris. The pmr 1 mutant in S. cerevisiae also displayed a slow growth phenotype when grown on low Ca2+ medium. The putative P. pastoris PMR1 gene, encoding for a 924 amino acid P-type Ca2+ ATPase, was disrupted in P. pastoris and the secretion of horseradish peroxidase (HRP) and β-gala
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16

Orman, Mehmet Ali. "Extracellular Recombinant Human Growth Hormone Production By Pichia Pastoris." Master's thesis, METU, 2007. http://etd.lib.metu.edu.tr/upload/3/12608616/index.pdf.

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In this study, the effects of bioprocess operation parameters on recombinant human growth hormone (rhGH) production by P. pastoris were systematically investigated. In this frame, first, for the extracellular expression and purification of human growth hormone by recombinant P. pastoris the cDNA of hGH, fused with a polyhistidine tag and also fused with a target site for the Factor Xa protease in which cleavage produces a mature N- and C- termini of rhGH, was cloned into pPICZ&amp<br>#945<br>A plasmid and the constructed system within the plasmid, pPICZ&amp<br>#945<br>A::hGH, was integrated to
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17

Čiplys, Evaldas. "Žmogaus virusų paviršiaus glikoproteinų ekspresijos tyrimas mielėse Pichia pastoris." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2007~D_20101125_183218-56493.

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Vienas pagrindinių biomedicininės paskirties baltymų gamybos iššūkių yra pigių ir saugių ekspresijos sistemų, tinkamų glikoproteinų sintezei, paieška bei esamų sistemų tobulinimas. Vaistai, sukurti baltymų pagrindu, sudaro apie ketvirtadalį naujai patvirtinamų vaistų rinkos, o apie 60% jų sudaryti iš glikoproteinų. Dabar glikoproteinų sintezei naudojamos žinduolių kultūros turi keletą trūkumų. Jose gaunamų rekombinantinių baltymų kaina yra didelė, ribotas tūrinis našumas, ląstelės lėtai dauginasi ir auga, būna užkrėstos retrovirusais, gaunamas heterogeniškas produktas ir užima daug laiko sukur
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18

Strauss, Colin Earl, and University of Lethbridge Faculty of Arts and Science. "Development of Pichia pastoris as a ruminal escape vehicle." Thesis, Lethbridge, Alta. : University of Lethbridge, Faculty of Arts and Science, 2000, 2000. http://hdl.handle.net/10133/148.

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The yeast expression system Pichia pastoris was investigated as an encapsulation technology capable of serving as a rumen escape vehicle. Cellularly encapsulated protein is protected from the ruminal environment so long as the cell membrane, which surrounds and isolates the intracellular protein is physically intact. Intracellular expression of Green Fluorescent Protein (GFP) allows for the monitoring of cellular integrity as necessary for the protection of encapsulated protein from ruminal proteases. Upon cellular lysis GFP is exposed to extracellular proteases which result in both the proteo
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19

Kongsaeree, Puapong Kelly Christine. "Optimization of recombinant ligninolytic enzyme production in Pichia pastoris." Related electronic resource: Current Research at SU : database of SU dissertations, recent titles available full text, 2004. http://wwwlib.umi.com/cr/syr/main.

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20

Solà, i. Rodrigo Aina. "Estudi del metabolisme central del carboni de Pichia pastoris." Doctoral thesis, Universitat Autònoma de Barcelona, 2004. http://hdl.handle.net/10803/5303.

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Aquesta tesi s'emmarca en els àmbits la biologia de sistemes i l'enginyeria metabòlica, és a dir, en l'anàlisi quantitatiu de sistemes biològics complexes. En aquest estudi s'ha utilitzat el llevat metilotròfic Pichia pastoris com a organisme model, el qual ha adquirit durant els darrers anys una importància creixent en l'àmbit de la biotecnologia, mostrant una gran capacitat com a sistema de producció de proteïnes recombinants. L'objectiu principal de l'estudi ha estat establir tècniques i metodologies experimentals i computacionals d'anàlisi quantitativa de la fisiologia cel·lular al sistem
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21

Tomàs, Gamisans Màrius. "Developing strategies for systems metabolic engineering of Pichia pastoris." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/458538.

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Pichia pastoris s’ha convertit en una de les plataformes cel·lulars més utilitzades per a la producció de proteïnes recombinants i metabòlits d’alt valor afegit. En els darrers anys s’han aconseguit fites importants en l’anàlisi quantitativa a nivell de sistemes de la seva fisiologia. Aquesta gran quantitat d’informació ha permès desenvolupar models metabòlics a escala genòmica, que permeten el desenvolupament de noves estratègies per l’enginyeria de soques i de bioprocés. Amb anterioritat a aquest estudi s’havien publicat tres models metabòlics a escala genòmica per a P. pastoris. No obstant
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22

Emberson, Louise. "Characterisation of soluble human CD33 expressed in Pichia pastoris." Thesis, University of Kent, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.396398.

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23

Perry, Evan T. "Expression of Human Neutrophil Cathepsin G in Pichia pastoris." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/honors/225.

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Cathepsin G (CatG), a serine protease found in the azurophil granules of neutrophils, participates in killing engulfed microorganisms. CatG is a poorly understood enzyme, in part because it can only be obtained as mature enzyme purified from human blood, and because it seems to have dual specificity for chymotrypsin-like and trypsin-like substrates. Therefore, yeast Pichia pastoris was used to express immature recombinant human CatG to provide a source of the enzyme free of biohazards and to allow study of its dual specificity and C-terminal processing. To avoid potential cleavage by a yeast k
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24

Feitosa, Valker Araujo. "Produção de fragmento recombinante de anticorpo em Pichia pastoris." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/9/9134/tde-10062014-084638/.

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Foram estudados a composição e o pH do meio de cultivo para a produção do fragmento de anticorpo (scFv) anti-LDL(-), expresso em Pichia pastoris recombinante. Os experimentos que definiram a composição e pH do meio assim como a concentração inicial de células na fase de indução foram realizados em agitador orbital a 250 rpm, com temperatura de 30 ºC na fase de crescimento e 20 ºC na fase de indução, durante 72 horas, com adição diária de 1% (v/v) de metanol. Para modificação do meio foi realizado um planejamento experimental empregando como variáveis independentes: extrato de soja, casaminoáci
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Johnson, Sabrina D. "Characterization of the Pichia pastoris alcohol oxidase I promoter." Scholarly Commons, 2003. https://scholarlycommons.pacific.edu/uop_etds/575.

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The methylotrophic yeast, Pichia past oris, is one of the most respected and widely used systems today. The ability of this yeast to produce large masses of protein and metabolize methanol as a sole source of carbon and energy is attributed to the highly induceable Alcohol Oxidase I promoter (AOXI). Despite of the disperse popularity and use of this promoter over the last 15 years, little is known about the transcription controls at a molecular level. A 5'>3' deletion analysis of the AOXI promoter was perrormed to gain understanding of the promoter's regulation and provided insight to the appr
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Coleman, Ellen(Ellen M. ). "Establishment of a novel Pichia Pastoris host production platform." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/126948.

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Thesis: M.B.A., Massachusetts Institute of Technology, Sloan School of Management, in conjunction with the Leaders for Global Operations Program at MIT, May, 2020<br>Thesis: S.M., Massachusetts Institute of Technology, Department of Mechanical Engineering, in conjunction with the Leaders for Global Operations Program at MIT, May, 2020<br>Cataloged from the official PDF of thesis.<br>Includes bibliographical references (pages 61-62).<br>The Cell Line Development group at Amgen is responsible for manufacturing and optimizing the cell lines utilized in production of Amgen's biologic drug portfoli
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Cochran, Keith Jacob. "Combined fermentation and recovery using expanded bed chromatography." Worcester, Mass. : Worcester Polytechnic Institute, 2006. http://www.wpi.edu/Pubs/ETD/Available/etd-081806-184321/.

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28

Lange, Stefan. "Etablierung von neuen Methoden zur Herstellung rekombinanter Antikörper und zur spezifischen Selektion von Antikörpervarianten im hohen Durchsatz." [S.l. : s.n.], 2002. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB10073673.

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29

STORCH, Otávio Brod. "Avaliação como probióticos para frangos de corte de Pichia pastoris e Pichia pastoris recombinante contendo o gene da fosfolipase C de Clostridium perfringens." Universidade Federal de Pelotas, 2008. http://repositorio.ufpel.edu.br/handle/ri/2527.

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Made available in DSpace on 2014-08-20T14:37:56Z (GMT). No. of bitstreams: 1 dissertacao_otavio_brod.pdf: 418993 bytes, checksum: 32f13d3584050e87ad3df6842a8e5496 (MD5) Previous issue date: 2008-11-17<br>Antibiotics used for the last decades as growth promoters for animals, were banned in several countries because of the risk of inducing the appearance of antibiotic resistant bacteria. Probiotics, live microorganisms that benefit health promoting the stability of the gut microbiota, are their most promising substitutes. The objective of this research was to determine the probiotic properties
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Nepomuceno, Denise Rocha. "ExpressÃo em Escherichia coli e Pichia pastoris de uma quitinase antifÃngica da famÃlia 19 das glicosÃdeo hidrolases de Chromobacterium violaceum." Universidade Federal do CearÃ, 2012. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=9171.

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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico<br>Chromobacterium violaceum à uma bactÃria Gram-negativa, saprÃfita, nÃo patogÃnica e de vida livre. A anotaÃÃo do genoma dessa espÃcie revelou muitos genes codificando produtos com potenciais aplicaÃÃes em diversas Ãreas. Dentre esses produtos estÃo vÃrias quitinases, enzimas capazes de degradar quitina, um polissacarÃdeo de N-acetil-&#946;-D-glucosamina presente na parede celular de muitos fungos e no exoesqueleto de insetos e crustÃceos. As quitinases sÃo de grande interesse biotecnolÃgico, podendo ser utilizadas para converter
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Santana, Ana Carolina. "Expressão e caracterização das quimases recombinantes específicas de mastócitos de camundongos (mMCP4 e 5)." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/17/17136/tde-18122014-150712/.

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Os mastócitos, em associação com outras células inflamatórias se acumulam em locais de tumor. Entretanto, pouco é conhecido sobre o papel exato dos mastócitos e de seus mediadores na progressão tumoral e angiogênese. Resultados recentes do nosso laboratório mostraram que a expressão das triptases específicas de mastócitos está correlacionada com a progressão tumoral (de Souza, Jr, PLosOne 7(7): e40790). Além do mais, existe uma associação entre mastócitos e a angiogênese tumoral. Então, tornou-se interessante investigar o papel das quimases específicas de mastócitos (mMCP-4 e -5) neste process
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Monforte, Mercado Sergi. "Systems metabolic engineering for recombinant protein production in Pichia pastoris." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/669385.

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El llevat metilotròfic Pichia pastoris (Komagataella sp.) és un dels sistemes d’expressió més atractius per a la producció de proteïna recombinant, mercat contínuament en expansió. El fort promotor del gen de l’alcohol oxidasa 1 (PAOX1), induït per metanol però reprimit per glucosa, glicerol o etanol, és un dels més emprats per aquest propòsit. No obstant, existeixen encara diversos colls d’ampolla fisiològics que limitant el procés. En aquest context, diferents estratègies han estat proposades i provades per tal de millorar la producció heteròloga de molts tipus diferents de proteïna. Les ap
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Cornelissen, Gesine. "Integrierte Bioprozessentwicklung zur Herstellung pharmakologischer wirksamer Proteine mit Pichia pastoris /." Düsseldorf : VDI-Verl, 2004. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=012990697&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.

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34

Panagiotou, Vasiliki. "Clonal selection and characterization of epigenetic variation in Pichia pastoris." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/59881.

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Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2010.<br>Page 71 blank. Cataloged from PDF version of thesis.<br>Includes bibliographical references (p. 67-70).<br>Recombinant proteins produced by different host organisms have been broadly used as therapeutics. Considering the demand for large quantities of protein drugs, methods are needed to increase reactor titers in a timely and cost-effective manner. We used random chemical mutagenesis to modify a wild-type strain of the heterologous protein production host Pichia pastoris, which resulted in overall im
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35

Thor, Der. "Cloning and characterization of MET2 in Pichia pastoris : a thesis." Scholarly Commons, 2001. https://scholarlycommons.pacific.edu/uop_etds/570.

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The methylotrophic yeast, Pichia pastoris, has been used as a protein expression system to express over 500 heterologous proteins. P. pastoris provides many advantages over other organisms that have been utilized for this purpose. In this project, we developed a new host/selectable marker and auxotrophic strains of P. pastoris based on methionine biosynthesis to increase P. pastoris's versatility as a host for homologous protein expression. This was accomplished by selecting for a yeast that is deficient in methionine biosynthesis, P. pastoris (yJC239), and gene complementation through transfo
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36

Moy, Allison. "Comparison of zeocin and G418 resistance markers in Pichia pastoris." Scholarly Commons, 2009. https://scholarlycommons.pacific.edu/uop_etds/736.

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37

Batista, Vinícius Daniel Ferreira. "Construção de um vetor para expressão heteróloga em Pichia pastoris." reponame:Repositório Institucional da UnB, 2012. http://repositorio.unb.br/handle/10482/11731.

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Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celular, Programa de Pós-Graduação em Biologia Molecular, 2012.<br>Submitted by Alaíde Gonçalves dos Santos (alaide@unb.br) on 2012-11-28T12:00:31Z No. of bitstreams: 1 2012_ViniciusDanielFerreiraBatista.pdf: 1512646 bytes, checksum: e4fb096aa8b82d64fbc0c08c52e23512 (MD5)<br>Approved for entry into archive by Guimaraes Jacqueline(jacqueline.guimaraes@bce.unb.br) on 2012-12-03T14:18:31Z (GMT) No. of bitstreams: 1 2012_ViniciusDanielFerreiraBatista.pdf: 1512646 bytes, checksum: e4fb096aa8b
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Valença, Iara Holanda. "Produção heteróloga do peptídeo antimicrobiano AFP 1.5 em Pichia pastoris." reponame:Repositório Institucional da UnB, 2015. http://repositorio.unb.br/handle/10482/20307.

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Dissertação (mestrado)—Universidade de Brasília, Instituto de Química, 2015.<br>Submitted by Fernanda Percia França (fernandafranca@bce.unb.br) on 2015-12-03T16:47:27Z No. of bitstreams: 1 2015_IaraHolandaValença.pdf: 1038288 bytes, checksum: 2c2ec6fed1c12e7de341a325f0860f59 (MD5)<br>Approved for entry into archive by Raquel Viana(raquelviana@bce.unb.br) on 2016-05-19T16:57:38Z (GMT) No. of bitstreams: 1 2015_IaraHolandaValença.pdf: 1038288 bytes, checksum: 2c2ec6fed1c12e7de341a325f0860f59 (MD5)<br>Made available in DSpace on 2016-05-19T16:57:38Z (GMT). No. of bitstreams: 1 2015_IaraHolandaVa
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Araújo, Juliana de Amorim. "Produção de quimosina B de Bos taurus em Pichia pastoris." reponame:Repositório Institucional da UnB, 2008. http://repositorio.unb.br/handle/10482/1328.

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Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celular, 2008.<br>Submitted by Jaqueline Oliveira (jaqueoliveiram@gmail.com) on 2008-12-11T19:02:46Z No. of bitstreams: 1 DISSERTACAO_2008_JulianaAmorimAraujo.pdf: 3037793 bytes, checksum: a7399845548557e765e50c02f6d3d986 (MD5)<br>Approved for entry into archive by Georgia Fernandes(georgia@bce.unb.br) on 2009-02-20T15:27:14Z (GMT) No. of bitstreams: 1 DISSERTACAO_2008_JulianaAmorimAraujo.pdf: 3037793 bytes, checksum: a7399845548557e765e50c02f6d3d986 (MD5)<br>Made available in DSpace
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40

Silva, Pedro Ricardo Ferreira. "Optimização da produção de scFv anti-LDL(-) por Pichia pastoris." Master's thesis, Universidade de Aveiro, 2013. http://hdl.handle.net/10773/11450.

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Mestrado em Biotecnologia<br>Foram avaliadas, em frasco Erlenmeyer e em bio-reactor, as vantagens da alimentação escalonada de fontes de carbono, glicerol e sorbitol, complementares ao metanol na fase de indução de produção. A concentração máxima do fragmento de anticorpo monoclonal recombinante scFv anti-LDL(-) obtida após 120h de tempo de cultivo foi de 148,7 ± 18,3 mg.L-1 para os cultivos aos quais foi adicionado glicerol, assim como a concentração celular mais elevada no final do cultivo, de 15,6 ± 1,1 g.L-1. Nos estudos realizados em bio-reactor, observou-se uma taxa específica de crescim
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41

Bispo, Sara Filipa Rato. "Analysis of glycosylation heterogeneity in antibody production by Pichia pastoris." Master's thesis, FCT - UNL, 2009. http://hdl.handle.net/10362/2384.

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Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção do grau de Mestre em Biotecnologia<br>The aims of this M.Sc. thesis are the production of an antibody fragment in Pichia pastoris and to assess O-glycosylation heterogeneity theoretically and experimentally. All possible glycoforms (glycans that are attached to proteins) attached to this antibody were calculated and a simple mathematical model was developed in MATLAB to predict glycoforms heterogeneity. The production of Anti-(ED-B) scFv by Pichia pastoris was made in a 50 L fedbatch
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42

Nguyen, Jack. "Development of a high throughput reporter system using β-Galactosidase in the yeast : Pichia Pastoris". Scholarly Commons, 2005. https://scholarlycommons.pacific.edu/uop_etds/623.

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Pichia pastoris is a methylotrophic yeast gaining acclamation for its capabili ties in heterologous protein expression. In contrast to other host organisms such as bacteria or mammalian cells, P. pastoris offers many advantages over its counterparts. For example, P. pastoris is cost-effective in that it can grow to high cell densities on simple media. The optional use of a constitutive (GAP) or inducible (A OXI) promoter and the ab ility to perfo1m post-translational protein modifications are additional qualities that make for a powerful heterologous expression system. This study focuses on ha
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43

Neto, AntÃnio Viana Lopes. "Atividade fungistÃtica de uma quitinase recombinante do feijÃo de corda [Vigna unguiculata (L.) (Walp.)] contra Lasiodiplodia theobromae Pat. (Griff. e Maubl.), agente causal da resinose do cajueiro (Anacardium occidentale L.)." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13310.

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CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior<br>O objetivo deste trabalho foi avaliar a atividade biolÃgica de uma quitinase recombinante (rVuChi) de feijÃo-caupi (Vigna unguiculata) contra o fungo fitopatogÃnico Lasiodiplodia theobromae. A proteÃna recombinante foi expressa em Pichia pastoris, coletada e purificada apÃs 72h de induÃÃo, utilizando cromatografia de afinidade em matriz de quitina. A quitinase foi eluÃda a partir da cromatografia de afinidade com Ãcido acÃtico a 0,1 M. Ensaio enzimÃtico foi realizado contra o substrato sintÃtico (quitina coloidal), a fim de determin
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44

Branco, Juliana Inês. "Avaliação da imunogenicidade de diferentes formas alélicas da proteí­na recombinante PvAMA-1expressa em Pichia pastoris: impacto da diversidade antigênica." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/9/9142/tde-12112018-145334/.

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A malária é um problema de saúde pública no Brasil e no mundo. Em 2016, o número de casos estimado pela Organização Mundial de Saúde foi de 216 milhões. Plasmodium falciparum é a espécie mais prevalente e responsável pelo maior número de mortes no mundo, sobretudo no continente africano. Por outro lado, o Plasmodium vivax é conhecido por sua ampla distribuição geográfica, sendo a espécie que predomina nas Américas, incluindo o Brasil. Nos últimos 20 anos, nosso grupo tem gerado e caracterizado diversas proteínas recombinantes baseadas em antígenos imunodominantes de P. vivax que podem servir c
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45

Ata, Ozge. "Metabolical Engineering Of Pichia Pastoris For Extracellular Thermostable Glucose Isomerase Production." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614596/index.pdf.

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The aim of this study is to develop a metabolically engineered P. pastoris strain for production of an active extracellular thermostable glucose isomerase (GI) enzyme by using genetic engineering techniques. For this purpose, research program was performed in two sub-programs. In the first sub-program, xylA gene from Thermus thermophilus was amplified and inserted into pPICZ&alpha<br>-A expression vector. Thereafter, this pPICZ&alpha<br>-A::xylA vector was cloned into AOX1 locus in P. pastoris genome and expressed under alcohol oxidase promoter which is induced by methanol. After constructing
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46

Kelle, Sebastian [Verfasser]. "Heterologe Expression von Enzymen aus Basidiomyceten in Pichia pastoris / Sebastian Kelle." Hannover : Technische Informationsbibliothek (TIB), 2015. http://d-nb.info/1084239795/34.

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47

Gu, Lina. "Heterologous expression of manganese peroxidase from Phanerochaete chrysosporium in Pichia pastoris." Related Electronic Resource: Current Research at SU : database of SU dissertations, recent titles available full text, 2003. http://wwwlib.umi.com/cr/syr/main.

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48

Santos, de Jesus Sérgio. "Análisis cuantitativo y modelización del metabolismo de la levadura Pichia pastoris." Doctoral thesis, Universitat Autònoma de Barcelona, 2008. http://hdl.handle.net/10803/5324.

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El presente trabajo está centrado en el análisis y modelización del metabolismo central de la levadura Pichia pastoris. Concretamente, el objetivo de este trabajo consistió en analizar la distribución de flujos en las principales vías metabólicas del metabolismo central de esta levadura mediante distintas aproximaciones experimentales y matemáticas basadas en un modelo metabólico estequiométrico y compartimentalizado. Los datos experimentales fueron obtenidos en su mayor parte del trabajo de tesis de A. Solà (Solà, 2004, tesis doctoral, Universitat Autònoma de Barcelona), centrado en experimen
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49

Barrero, Peña Juan José. "Overcoming the secretory limitations in Pichia pastoris for recombinant protein production." Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2020. http://hdl.handle.net/10803/670387.

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El llevat metilotròfic Pichia pastoris (genere Komagataella) s’ha convertit en una de les plataformes cel·lulars més populars per produir proteïnes d’interès industrial, la majoria de les quals es secreten extracel·lularment per facilitar el posterior procés de purificació. La secreció de proteïnes heteròlogues fora de la cèl·lula està mediada per la via secretora, una ruta que engloba diversos passos i té diferents orgànuls interconnectats per aconseguir una secreció eficient. Tanmateix, quan P. pastoris es veu obligat a produir proteïnes heteròlogues, el correcte funcionament de la via de se
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50

Bergeld, Linnéa. "Trametes versicolor laccase: random mutagenesis and heterologous expression in Pichia pastoris." Thesis, Karlstad University, Division for Chemistry, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-1580.

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<p>Laccase is a blue multi-copper oxidase. It has a broad biotechnical potential which increases the interest to study the enzyme further. A laccase-encoding gene from the white-rot fungus Trametes versicolor (lcc2) was mutated using two different methods for random mutagenesis: error-prone PCR and a method based on an E.coli strain (ES1301 mutS) that introduces random mutations. For the error-prone PCR reaction, the vector pPICZB with the lcc2 gene inserted was used as template. The E. coli strain ES1301 mutS was transformed with the vector pBluescript SKII with the lcc2 gene as insert. The m
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