Log in

Relevant bibliographies by topics / Pichia Pastoris N-Glycosylation / Journal articles

To see the other types of publications on this topic, follow the link: Pichia Pastoris N-Glycosylation.

Journal articles on the topic 'Pichia Pastoris N-Glycosylation'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 September 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Pichia Pastoris N-Glycosylation.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Vervecken, Wouter, Vladimir Kaigorodov, Nico Callewaert, Steven Geysens, Kristof De Vusser, and Roland Contreras. "In Vivo Synthesis of Mammalian-Like, Hybrid-Type N-Glycans in Pichia pastoris." Applied and Environmental Microbiology 70, no. 5 (2004): 2639–46. http://dx.doi.org/10.1128/aem.70.5.2639-2646.2004.

Full text

Abstract:

ABSTRACT The Pichia pastoris N-glycosylation pathway is only partially homologous to the pathway in human cells. In the Golgi apparatus, human cells synthesize complex oligosaccharides, whereas Pichia cells form mannose structures that can contain up to 40 mannose residues. This hypermannosylation of secreted glycoproteins hampers the downstream processing of heterologously expressed glycoproteins and leads to the production of protein-based therapeutic agents that are rapidly cleared from the blood because of the presence of terminal mannose residues. Here, we describe engineering of the P. p

APA, Harvard, Vancouver, ISO, and other styles

2

Bretthauer, Roger K. "Genetic engineering of Pichia pastoris to humanize N-glycosylation of proteins." Trends in Biotechnology 21, no. 11 (2003): 459–62. http://dx.doi.org/10.1016/j.tibtech.2003.09.005.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Jacobs, Pieter P., Steven Geysens, Wouter Vervecken, Roland Contreras, and Nico Callewaert. "Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology." Nature Protocols 4, no. 1 (2008): 58–70. http://dx.doi.org/10.1038/nprot.2008.213.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Kallas, Åsa M., Kathleen Piens, Stuart E. Denman, et al. "Enzymatic properties of native and deglycosylated hybrid aspen (Populus tremula×tremuloides) xyloglucan endotransglycosylase 16A expressed in Pichia pastoris." Biochemical Journal 390, no. 1 (2005): 105–13. http://dx.doi.org/10.1042/bj20041749.

Full text

Abstract:

The cDNA encoding a xyloglucan endotransglycosylase, PttXET16A, from hybrid aspen (Populus tremula×tremuloides) has been isolated from an expressed sequence tag library and expressed in the methylotrophic yeast Pichia pastoris. Sequence analysis indicated a high degree of similarity with other proteins in the XTH (xyloglucan transglycosylase/hydrolase) gene subfamily of GH16 (glycoside hydrolase family 16). In addition to the conserved GH16 catalytic sequence motif, PttXET16A contains a conserved N-glycosylation site situated proximal to the predicted catalytic residues. MS analysis indicated

APA, Harvard, Vancouver, ISO, and other styles

5

Giersing, Birgitte, Kazutoyo Miura, Richard Shimp, et al. "Posttranslational Modification of Recombinant Plasmodium falciparum Apical Membrane Antigen 1: Impact on Functional Immune Responses to a Malaria Vaccine Candidate." Infection and Immunity 73, no. 7 (2005): 3963–70. http://dx.doi.org/10.1128/iai.73.7.3963-3970.2005.

Full text

Abstract:

ABSTRACT Recombinant apical membrane antigen 1 (AMA1) is a leading vaccine candidate for Plasmodium falciparum malaria, as antibodies against recombinant P. falciparum AMA1 (PfAMA1) interrupt merozoite invasion into erythrocytes. In order to investigate the role of posttranslational modification in modulating the functional immune response to recombinant AMA1, two separate alleles of PfAMA1 (FVO and 3D7), in which native N-glycosylation sites have been mutated, were produced using Escherichia coli and a Pichia pastoris expression system. Recombinant Pichia pastoris AMA1-FVO (PpAMA1-FVO) and Pp

APA, Harvard, Vancouver, ISO, and other styles

6

Kukk, Kaia, Sergo Kasvandik, and Nigulas Samel. "N-glycosylation site occupancy in human prostaglandin H synthases expressed in Pichia pastoris." SpringerPlus 3, no. 1 (2014): 436. http://dx.doi.org/10.1186/2193-1801-3-436.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Li, Siqiang, Peng Sun, Xin Gong, et al. "Engineering O-glycosylation in modified N-linked oligosaccharide (Man12GlcNAc2∼Man16GlcNAc2) Pichia pastoris strains." RSC Advances 9, no. 15 (2019): 8246–52. http://dx.doi.org/10.1039/c8ra08121b.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Peng, Huakang, Mengqi Wang, Nan Wang та ін. "Different N-Glycosylation Sites Reduce the Activity of Recombinant DSPAα2". Current Issues in Molecular Biology 44, № 9 (2022): 3930–47. http://dx.doi.org/10.3390/cimb44090270.

Full text

Abstract:

Bat plasminogen activators α2 (DSPAα2) has extremely high medicinal value as a powerful natural thrombolytic protein. However, wild-type DSPAα2 has two N-glycosylation sites (N185 and N398) and its non-human classes of high-mannose-type N-glycans may cause immune responses in vivo. By mutating the N-glycosylation sites, we aimed to study the effect of its N-glycan chain on plasminogen activation, fibrin sensitivity, and to observe the physicochemical properties of DSPAα2. A logical structure design was performed in this study. Four single mutants and one double mutant were constructed and expr

APA, Harvard, Vancouver, ISO, and other styles

9

Han, Minghai, Xinfeng Wang, Guilong Yan, et al. "Modification of recombinant elastase expressed in Pichia pastoris by introduction of N-glycosylation sites." Journal of Biotechnology 171 (February 2014): 3–7. http://dx.doi.org/10.1016/j.jbiotec.2013.11.021.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Peng, Huakang, Nan Wang, Mengqi Wang та ін. "Comparison of Activity and Safety of DSPAα1 and Its N-Glycosylation Mutants". Life 13, № 4 (2023): 985. http://dx.doi.org/10.3390/life13040985.

Full text

Abstract:

DSPAα1 is a potent rude thrombolytic protein with high medicative value. DSPAα1 has two natural N-glycan sites (N153Q-S154-S155, N398Q-K399-T400) that may lead to immune responses when administered in vivo. We aimed to study the effect of its N-glycosylation sites on DSPAα1 in vitro and in vivo by mutating these N-glycosylation sites. In this experiment, four single mutants and one double mutant were predicted and expressed in Pichia pastoris. When the N398Q-K399-T400 site was mutated, the fibrinolytic activity of the mutant was reduced by 75%. When the N153Q-S154-S155 sites were inactivated a

APA, Harvard, Vancouver, ISO, and other styles

11

BORASTON, Alisdair B., R. Antony J. WARREN, and Douglas G. KILBURN. "Glycosylation by Pichia pastoris decreases the affinity of a family 2a carbohydrate-binding module from Cellulomonas fimi: a functional and mutational analysis." Biochemical Journal 358, no. 2 (2001): 423–30. http://dx.doi.org/10.1042/bj3580423.

Full text

Abstract:

When produced by Pichia pastoris, three of the five Asn-Xaa-Ser/Thr sequences (corresponding to Asn-24, Asn-73 and Asn-87) in the carbohydrate-binding module CBM2a of xylanase 10A from Cellulomonas fimi are glycosylated. The glycans are of the high-mannose type, ranging in size from GlcNAc2Man8 to GlcNAc2Man14. The N-linked glycans block the binding of CBM2a to cellulose. Analysis of mutants of CBM2a shows that glycans on Asn-24 decrease the association constant (Ka) for the binding of CBM2a to bacterial microcrystalline cellulose approx. 10-fold, whereas glycans on Asn-87 destroy binding. The

APA, Harvard, Vancouver, ISO, and other styles

12

Choi, B. K., P. Bobrowicz, R. C. Davidson, et al. "Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris." Proceedings of the National Academy of Sciences 100, no. 9 (2003): 5022–27. http://dx.doi.org/10.1073/pnas.0931263100.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Yoshimasu, M. A. "Effect of N-linked glycosylation on the aspartic proteinase porcine pepsin expressed from Pichia pastoris." Glycobiology 14, no. 5 (2004): 417–29. http://dx.doi.org/10.1093/glycob/cwh024.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Zou, Shuping, Shen Huang, Imdad Kaleem та Chun Li. "N-glycosylation enhances functional and structural stability of recombinant β-glucuronidase expressed in Pichia pastoris". Journal of Biotechnology 164, № 1 (2013): 75–81. http://dx.doi.org/10.1016/j.jbiotec.2012.12.015.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Wang, Zhiyan, Chao Guo, Lin Liu, and He Huang. "Effects of N-glycosylation on the biochemical properties of recombinant bEKL expressed in Pichia pastoris." Enzyme and Microbial Technology 114 (July 2018): 40–47. http://dx.doi.org/10.1016/j.enzmictec.2018.03.004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Marbach, Jendrik, Peter Zentis, Philipp Ellinger, Henrik Müller, and Eva Birkmann. "Expression and characterisation of fully posttranslationally modified cellular prion protein in Pichia pastoris." Biological Chemistry 394, no. 11 (2013): 1475–83. http://dx.doi.org/10.1515/hsz-2013-0180.

Full text

Abstract:

Abstract Prion diseases are fatal neurodegenerative diseases which occur as sporadic, genetic, and transmissible disorders. A molecular hallmark of prion diseases is the conformational conversion of the host-encoded cellular form of the prion protein (PrPC) into its misfolded pathogenic isoform (PrPSc). PrPSc is the main component of the pathological and infectious prion agent. The study of the conversion mechanism from PrPC to PrPSc is a major field in prion research. PrPC is glycosylated and attached to the plasma membrane via its glycosyl phosphatidyl inositol (GPI)-anchor. In this study we

APA, Harvard, Vancouver, ISO, and other styles

17

Senerovic, Lidija, Nad Stankovic, G. Ljubijankic, and Branka Vasiljevic. "Glycosylation and pH stability of penicillin G acylase from providencia rettgeri produced in Pichia pastoris." Archives of Biological Sciences 61, no. 4 (2009): 581–86. http://dx.doi.org/10.2298/abs0904581s.

Full text

Abstract:

Penicillin G acylase (PAC) is one of the most widely used enzymes in industrial synthesis of semi-synthetic antibiotics. The Providencia rettgeri pac gene was expressed to a level of 2.7 U/ml using the Pichia pastoris expression system. The recombinant enzyme was purified and its glycosylation status was determined. It was found that both subunits (? and ?) of the enzyme were N-glycosylated, while the ?-subunit also contained O-glycans. It was also observed that rPACP.rett. was stable in a wide range of pH, which, in addition to the previously proved high thermostability, makes it an attractiv

APA, Harvard, Vancouver, ISO, and other styles

18

Kocken, Clemens H. M., Martin A. Dubbeld, Annemarie Van Der Wel, et al. "High-Level Expression of Plasmodium vivax Apical Membrane Antigen 1 (AMA-1) in Pichia pastoris: Strong Immunogenicity in Macaca mulatta Immunized with P. vivax AMA-1 and Adjuvant SBAS2." Infection and Immunity 67, no. 1 (1999): 43–49. http://dx.doi.org/10.1128/iai.67.1.43-49.1999.

Full text

Abstract:

ABSTRACT The apical membrane antigen 1 (AMA-1) family is a promising family of malaria blood-stage vaccine candidates that have induced protection in rodent and nonhuman primate models of malaria. Correct conformation of the protein appears to be essential for the induction of parasite-inhibitory responses, and these responses appear to be primarily antibody mediated. Here we describe for the first time high-level secreted expression (over 50 mg/liter) of thePlasmodium vivax AMA-1 (PV66/AMA-1) ectodomain by using the methylotrophic yeast Pichia pastoris. To prevent nonnative glycosylation, a c

APA, Harvard, Vancouver, ISO, and other styles

19

Liu, Yusi, Tamara Hoppenbrouwers, Yulu Wang, et al. "Glycosylation Contributes to Thermostability and Proteolytic Resistance of rFIP-nha (Nectria haematococca)." Molecules 28, no. 17 (2023): 6386. http://dx.doi.org/10.3390/molecules28176386.

Full text

Abstract:

Glycosylation is an important post-translational modification of proteins, contributing to protein function, stability and subcellular localization. Fungal immunomodulatory proteins (FIPs) are a group of small proteins with notable immunomodulatory activity, some of which are glycoproteins. In this study, the impact of glycosylation on the bioactivity and biochemical characteristics of FIP-nha (from Nectria haematococca) is described. Three rFIP-nha glycan mutants (N5A, N39A, N5+39A) were constructed and expressed in Pichia pastoris to study the functionality of the specific N-glycosylation on

APA, Harvard, Vancouver, ISO, and other styles

20

Kim, Eun Jae, Jun Hyuck Lee, Sung Gu Lee, and Se Jong Han. "Improving thermal hysteresis activity of antifreeze protein from recombinant Pichia pastoris by removal of N-glycosylation." Preparative Biochemistry & Biotechnology 47, no. 3 (2016): 299–304. http://dx.doi.org/10.1080/10826068.2016.1244682.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

WILLIAMS, Tracy A., Annie MICHAUD, Xavier HOUARD, Marie-Thérèse CHAUVET, Florent SOUBRIER, and Pierre CORVOL. "Drosophila melanogaster angiotensin I-converting enzyme expressed in Pichia pastoris resembles the C domain of the mammalian homologue and does not require glycosylation for secretion and enzymic activity." Biochemical Journal 318, no. 1 (1996): 125–31. http://dx.doi.org/10.1042/bj3180125.

Full text

Abstract:

Drosophila melanogaster angiotensin I-converting enzyme (AnCE) is a secreted single-domain homologue of mammalian angiotensin I-converting enzyme (ACE) which comprises two domains (N and C domains). In order to characterize in detail the enzymic properties of AnCE and to study the influence of glycosylation on the secretion and enzymic activity of this enzyme, we overexpressed AnCE (expression level, 160 mg/l) and an unglycosylated mutant (expression level, 43 mg/l) in the yeast Pichia pastoris. The recombinant enzyme was apparently homogeneous on SDS/PAGE without purification and partial degl

APA, Harvard, Vancouver, ISO, and other styles

22

HENRIKSSON, Hongbin, Stuart E. DENMAN, Iain D. G. CAMPUZANO, et al. "N-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16A." Biochemical Journal 375, no. 1 (2003): 61–73. http://dx.doi.org/10.1042/bj20030485.

Full text

Abstract:

The gene encoding a XET (xyloglucan endotransglycosylase) from cauliflower (Brassica oleracea var. botrytis) florets has been cloned and sequenced. Sequence analysis indicated a high degree of similarity to other XET enzymes belonging to glycosyl hydrolase family 16 (GH16). In addition to the conserved GH16 catalytic sequence motif EIDFE, there exists one potential N-linked glycosylation site, which is also highly conserved in XET enzymes from this family. Purification of the corresponding protein from extracts of cauliflower florets allowed the fractionation of a single, pure glycoform, which

APA, Harvard, Vancouver, ISO, and other styles

23

Wang, Nan, Kevin Yueju Wang, Fangfang Xu, GangQiang Li, and DeHu Liu. "The effect of N-glycosylation on the expression of the tetanus toxin fragment C in Pichia pastoris." Protein Expression and Purification 166 (February 2020): 105503. http://dx.doi.org/10.1016/j.pep.2019.105503.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Han, Minghai, Weixian Wang, Gongcheng Jiang, et al. "Enhanced expression of recombinant elastase in Pichia pastoris through addition of N-glycosylation sites to the propeptide." Biotechnology Letters 36, no. 12 (2014): 2467–71. http://dx.doi.org/10.1007/s10529-014-1620-4.

Full text

APA, Harvard, Vancouver, ISO, and other styles

25

Urbatsch, Ina L., Susan Wilke-Mounts, Khursheed Gimi, and Alan E. Senior. "Purification and Characterization of N-glycosylation Mutant Mouse and Human P-glycoproteins Expressed in Pichia pastoris Cells." Archives of Biochemistry and Biophysics 388, no. 1 (2001): 171–77. http://dx.doi.org/10.1006/abbi.2001.2299.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Verhülsdonk, Lukas, Hans Georg Mannherz, and Markus Napirei. "Comparison of the secretory murine DNase1 family members expressed in Pichia pastoris." PLOS ONE 16, no. 7 (2021): e0253476. http://dx.doi.org/10.1371/journal.pone.0253476.

Full text

Abstract:

Soluble nucleases of the deoxyribonuclease 1 (DNase1) family facilitate DNA and chromatin disposal (chromatinolysis) during certain forms of cell differentiation and death and participate in the suppression of anti-nuclear autoimmunity as well as thrombotic microangiopathies caused by aggregated neutrophil extracellular traps. Since a systematic and direct comparison of the specific activities and properties of the secretory DNase1 family members is still missing, we expressed and purified recombinant murine DNase1 (rmDNase1), DNase1-like 2 (rmDNase1L2) and DNase1-like 3 (rmDNase1L3) using Pic

APA, Harvard, Vancouver, ISO, and other styles

27

Trimble, R. B. "Characterization of N- and O-linked glycosylation of recombinant human bile salt-stimulated lipase secreted by Pichia pastoris." Glycobiology 14, no. 3 (2003): 265–74. http://dx.doi.org/10.1093/glycob/cwh036.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Xu, Ganfei, Yawen Wu, Yinliang Zhang, Wei Fang, Yazhong Xiao, and Zemin Fang. "Role of N-glycosylation on the specific activity of a Coprinopsis cinerea laccase Lcc9 expressed in Pichia pastoris." Journal of Bioscience and Bioengineering 128, no. 5 (2019): 518–24. http://dx.doi.org/10.1016/j.jbiosc.2019.05.004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Tull, Dedreia, Tine E. Gottschalk, Ib Svendsen та ін. "Extensive N-Glycosylation Reduces the Thermal Stability of a Recombinant Alkalophilic Bacillus α-Amylase Produced in Pichia pastoris". Protein Expression and Purification 21, № 1 (2001): 13–23. http://dx.doi.org/10.1006/prep.2000.1348.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Srivastava, Akriti, Pallavi Somvanshi, and Bhartendu Nath Mishra. "Reconstruction and visualization of carbohydrate, N-glycosylation pathways in Pichia pastoris CBS7435 using computational and system biology approaches." Systems and Synthetic Biology 7, no. 1-2 (2012): 7–22. http://dx.doi.org/10.1007/s11693-012-9102-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Guerrero-Olazarán, Martha, Lilí Rodríguez-Blanco, J. Gerardo Carreon-Treviño, Juan A. Gallegos-López, and José M. Viader-Salvadó. "Expression of a Bacillus Phytase C Gene in Pichia pastoris and Properties of the Recombinant Enzyme." Applied and Environmental Microbiology 76, no. 16 (2010): 5601–8. http://dx.doi.org/10.1128/aem.00762-10.

Full text

Abstract:

ABSTRACT The cloning and expression of a native gene encoding a Bacillus subtilis phytase using Pichia pastoris as the host is described. In addition, the influence of N-glycosylation on the biochemical properties of the B. subtilis phytase, the influence of pH on the thermostability of the recombinant and native B. subtilis phytases, and the resistance of both phytases to shrimp digestive enzymes and porcine trypsin are also described. After 48 h of methanol induction in shake flasks, a selected recombinant strain produced and secreted 0.82 U/ml (71 mg/liter) recombinant phytase. This phytase

APA, Harvard, Vancouver, ISO, and other styles

32

Garcia-Oliva, Cecilia, Pilar Hoyos, Lucie Petrásková та ін. "Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation". International Journal of Molecular Sciences 20, № 24 (2019): 6181. http://dx.doi.org/10.3390/ijms20246181.

Full text

Abstract:

Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest. The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications. It can be heterologously produced in Pichia pastoris in a high yield. The mutation of the Tyr470 residue to histidine greatly enhances its transglycosylation capability. The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase fo

APA, Harvard, Vancouver, ISO, and other styles

33

Han, Minghai, Xinfeng Wang, Huaiyu Ding, et al. "The role of N-glycosylation sites in the activity, stability, and expression of the recombinant elastase expressed by Pichia pastoris." Enzyme and Microbial Technology 54 (January 2014): 32–37. http://dx.doi.org/10.1016/j.enzmictec.2013.09.014.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Shi, Xiao-Wei, Ming-Lv Sun, Bo Zhou, and Xiao-Yun Wang. "Identification, characterization, and overexpression of a phytase with potential industrial interest." Canadian Journal of Microbiology 55, no. 5 (2009): 599–604. http://dx.doi.org/10.1139/w09-008.

Full text

Abstract:

A high phytase-producing strain of Aspergillus niger N-3 was identified by screening 104 microbial strains. The gene for A. niger N-3 was cloned and expressed in Pichia pastoris . The coding region without the introns and putative signal sequence was comprised of 1347 nucleotides. It encoded a polypeptide of 448 amino acids, exhibiting high amino acid sequence homologies (94.87%) with the typical phytase of A. niger NRRL 3135. The molecular mass of the recombinant phytase as determined by SDS–PAGE was 60–70 kDa, with maximum activity at ~55 °C (after incubation at 10 min). The phytase retained

APA, Harvard, Vancouver, ISO, and other styles

35

Kocken, Clemens H. M., Chrislaine Withers-Martinez, Martin A. Dubbeld, et al. "High-Level Expression of the Malaria Blood-Stage Vaccine Candidate Plasmodium falciparum Apical Membrane Antigen 1 and Induction of Antibodies That Inhibit Erythrocyte Invasion." Infection and Immunity 70, no. 8 (2002): 4471–76. http://dx.doi.org/10.1128/iai.70.8.4471-4476.2002.

Full text

Abstract:

ABSTRACT Apical membrane antigen 1 (AMA-1) is a highly promising malaria blood-stage vaccine candidate that has induced protection in rodent and nonhuman primate models of malaria. Authentic conformation of the protein appears to be essential for the induction of parasite-inhibitory antibody responses. Here we have developed a synthetic gene with adapted codon usage to allow expression of Plasmodium falciparum FVO strain AMA-1 (PfAMA-1) in Pichia pastoris. In addition, potential N-glycosylation sites were changed, exploiting the lack of conservation of these sites in Plasmodium, to obtain high

APA, Harvard, Vancouver, ISO, and other styles

36

Bach, Cao Xuan, Dang Thi Kim Anh, Nguyen Thanh Thuy, Truong Tu Anh, Nguyen Thi Dieu Linh, and Vu Nguyen Thanh. "Cloning of sucrose isomerase encoding gene from Klebsiella singaporensis ISB-36 and its expression in Pichia pastoris." Vietnam Journal of Biotechnology 17, no. 4 (2020): 749–56. http://dx.doi.org/10.15625/1811-4989/17/4/14722.

Full text

Abstract:

Given potential health benefits including low glycemic index, tooth friendly, suitable to infants, elderly and diabetic patients, isomaltulose was considered as a promising alternative sweetener to sucrose. Due to the presence of liposaccharide endotoxin in Serratia plymuthica CBS 574.44, a Gram-negative bacterium, and minute amount of formaldehyde carried over, purification of isomaltulose requires rigorous controls in industry. To reduce the cost associated with product purification, here we propose the use of recombinant enzyme in isomaltulose production. The mature gene coding for sucrose

APA, Harvard, Vancouver, ISO, and other styles

37

CALABOZO, Belén, Araceli DÍAZ-PERALES, Gabriel SALCEDO, Domingo BARBER, and Florentino POLO. "Cloning and expression of biologically active Plantago lanceolata pollen allergen Pla l 1 in the yeast Pichia pastoris." Biochemical Journal 372, no. 3 (2003): 889–96. http://dx.doi.org/10.1042/bj20021491.

Full text

Abstract:

The glycoprotein Pla l 1 is the major allergen from English plantain (Plantago lanceolata) pollen, which is a common cause of pollinosis in temperate areas. Three complete cDNAs for Pla l 1 isoforms were isolated by PCR using specific 3′ and 5′ primers. All three Pla l 1 cDNAs code for a 25-residue leader peptide and a 131-residue mature protein that contains two polymorphic positions, an N-glycosylation site at position 107 and six cysteine residues involved in three disulphide bridges. The allergen variant Pla l 1.0101 was produced in Pichia pastoris at a yield of 20 mg per litre of culture

APA, Harvard, Vancouver, ISO, and other styles

38

Muller-Steffner, Hélène, Isabelle Kuhn, Manuela Argentini, and Francis Schuber. "Identification of the N-glycosylation sites on recombinant bovine CD38 expressed in Pichia pastoris: Their impact on enzyme stability and catalytic activity." Protein Expression and Purification 70, no. 2 (2010): 151–57. http://dx.doi.org/10.1016/j.pep.2009.10.003.

Full text

APA, Harvard, Vancouver, ISO, and other styles

39

Chang, Xiaoyu, Bo Xu, Yingguo Bai, et al. "Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus expressed in Pichia pastoris." PLOS ONE 12, no. 2 (2017): e0171111. http://dx.doi.org/10.1371/journal.pone.0171111.

Full text

APA, Harvard, Vancouver, ISO, and other styles

40

Benabdessalem, Chaouki, Houcemeddine Othman, Rym Ouni, et al. "N-glycosylation and homodimeric folding significantly enhance the immunoreactivity of Mycobacterium tuberculosis virulence factor CFP32 when produced in the yeast Pichia pastoris." Biochemical and Biophysical Research Communications 516, no. 3 (2019): 845–50. http://dx.doi.org/10.1016/j.bbrc.2019.06.140.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

WEISS, H. Markus, Winfried HAASE, Hartmut MICHEL та Helmut REILÄNDER. "Comparative biochemical and pharmacological characterization of the mouse 5HT5A 5-hydroxytryptamine receptor and the human β2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris". Biochemical Journal 330, № 3 (1998): 1137–47. http://dx.doi.org/10.1042/bj3301137.

Full text

Abstract:

Over the last few years, Pichia pastoris has been developed into a powerful expression system for a multitude of foreign genes. Here, we demonstrate that the P. pastoris expression system has similar power to the baculovirus expression system in high-level production of two G-protein-coupled receptors, the mouse 5HT5A 5-hydroxtryptamine receptor and the human β2-adrenergic receptor. Different expression plasmids were constructed in which the cDNAs of the two receptors were cloned under the transcriptional control of the highly inducible promoter of the P. pastoris alcohol oxidase 1 (AOX1) gene

APA, Harvard, Vancouver, ISO, and other styles

42

Applegate, Dianne, Liana Haraga, Kathe M. Hertzberg, et al. "The EC Domains of Human Fibrinogen420Contain Calcium Binding Sites But Lack Polymerization Pockets." Blood 92, no. 10 (1998): 3669–74. http://dx.doi.org/10.1182/blood.v92.10.3669.

Full text

Abstract:

Abstract The extended  (E) isoform unique to Fibrinogen420 (Fib420) is distinguished from the conventional  chain of Fibrinogen340 by the presence of an additional 236-residue carboxyl terminus globular domain (EC). A recombinant form of EC (rEC), having a predicted mass of 27,653 Daltons, was expressed in yeast (Pichia pastoris) and purified by anion exchange column chromatography. Purified rEC appears to be predominantly intact, as judged by N-terminal sequence analysis, mass spectral analysis of the C-terminal cyanogen bromide (CNBr) fragment, and comparison of recognition by epitope

APA, Harvard, Vancouver, ISO, and other styles

43

Applegate, Dianne, Liana Haraga, Kathe M. Hertzberg, et al. "The EC Domains of Human Fibrinogen420Contain Calcium Binding Sites But Lack Polymerization Pockets." Blood 92, no. 10 (1998): 3669–74. http://dx.doi.org/10.1182/blood.v92.10.3669.422k21_3669_3674.

Full text

Abstract:

The extended  (E) isoform unique to Fibrinogen420 (Fib420) is distinguished from the conventional  chain of Fibrinogen340 by the presence of an additional 236-residue carboxyl terminus globular domain (EC). A recombinant form of EC (rEC), having a predicted mass of 27,653 Daltons, was expressed in yeast (Pichia pastoris) and purified by anion exchange column chromatography. Purified rEC appears to be predominantly intact, as judged by N-terminal sequence analysis, mass spectral analysis of the C-terminal cyanogen bromide (CNBr) fragment, and comparison of recognition by epitope-specific

APA, Harvard, Vancouver, ISO, and other styles

44

Alcobaça, Olinda S. A., Emeline B. Campanini, Iara Ciancaglini та ін. "Identification of a New Endo-β-1,4-xylanase Prospected from the Microbiota of the Termite Heterotermes tenuis". Microorganisms 10, № 5 (2022): 906. http://dx.doi.org/10.3390/microorganisms10050906.

Full text

Abstract:

Xylanases are hemicellulases that break down xylan to soluble pentoses. They are used for industrial purposes, such as paper whitening, beverage clarification, and biofuel production. The second-generation bioethanol production is hindered by the enzymatic hydrolysis step of the lignocellulosic biomass, due to the complex arrangement established among its constituents. Xylanases can potentially increase the production yield by improving the action of the cellulolytic enzyme complex. We prospected endo-β-1,4-xylanases from meta-transcriptomes of the termite Heterotermes tenuis. In silico struct

APA, Harvard, Vancouver, ISO, and other styles

45

Alcobaça, Olinda S. A., Emeline B. Campanini, Iara Ciancaglini та ін. "Identification of a New Endo-β-1,4-xylanase Prospected from the Microbiota of the Termite Heterotermes tenuis". Microorganisms 10, № 5 (2022): 906. http://dx.doi.org/10.3390/microorganisms10050906.

Full text

Abstract:

Xylanases are hemicellulases that break down xylan to soluble pentoses. They are used for industrial purposes, such as paper whitening, beverage clarification, and biofuel production. The second-generation bioethanol production is hindered by the enzymatic hydrolysis step of the lignocellulosic biomass, due to the complex arrangement established among its constituents. Xylanases can potentially increase the production yield by improving the action of the cellulolytic enzyme complex. We prospected endo-β-1,4-xylanases from meta-transcriptomes of the termite Heterotermes tenuis. In silico struct

APA, Harvard, Vancouver, ISO, and other styles

46

Niu, Chengtuo, Yupeng Han, Jinjing Wang, et al. "Comparative analysis of the effect of protein Z4 from barley malt and recombinant Pichia pastoris on beer foam stability: Role of N-glycosylation and glycation." International Journal of Biological Macromolecules 106 (January 2018): 241–47. http://dx.doi.org/10.1016/j.ijbiomac.2017.08.001.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Bonaccorsi di Patti, Maria Carmela, Antimo Cutone, Marek Nemčovič, Zuzana Pakanová, Peter Baráth, and Giovanni Musci. "Production of Recombinant Human Ceruloplasmin: Improvements and Perspectives." International Journal of Molecular Sciences 22, no. 15 (2021): 8228. http://dx.doi.org/10.3390/ijms22158228.

Full text

Abstract:

The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin. Mutations in the CP gene give rise to aceruloplasminemia, a rare neurodegenerative disease for which no cure is available. Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies. Furthermore, recombinant CP could be evaluated for enzyme replacement therapy for the treatment of aceruloplasminemia. We report the production and preliminary characterization

APA, Harvard, Vancouver, ISO, and other styles

48

Salusjärvi, Tuomas, Nisse Kalkkinen, and Andrei N. Miasnikov. "Cloning and Characterization of Gluconolactone Oxidase of Penicillium cyaneo-fulvum ATCC 10431 and Evaluation of Its Use for Production of d-Erythorbic Acid in Recombinant Pichia pastoris." Applied and Environmental Microbiology 70, no. 9 (2004): 5503–10. http://dx.doi.org/10.1128/aem.70.9.5503-5510.2004.

Full text

Abstract:

ABSTRACT A d-erythorbic acid-forming soluble flavoprotein, gluconolactone oxidase (GLO), was purified from Penicillium cyaneo-fulvum strain ATCC 10431 and partially sequenced. Peptide sequences were used to isolate a cDNA clone encoding the enzyme. The cloned gene (accession no. AY576053 ) exhibits high levels of similarity with the genes encoding other known eukaryotic lactone oxidases and also with the genes encoding some putative prokaryotic lactone oxidases. Analysis of the coding sequence of the GLO gene indicated the presence of a typical secretion signal sequence at the N terminus of GL

APA, Harvard, Vancouver, ISO, and other styles

49

Muraki, Michiro. "Secretory expression of synthetic human Fas ligand extracellular domain gene in Pichia pastoris: Influences of tag addition and N-glycosylation site deletion, and development of a purification method." Protein Expression and Purification 50, no. 2 (2006): 137–46. http://dx.doi.org/10.1016/j.pep.2006.08.006.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Degani, Genny, Alberto Barbiroli, Paula Magnelli, et al. "Insights into the effects of N-glycosylation on the characteristics of the VC1 domain of the human receptor for advanced glycation end products (RAGE) secreted by Pichia pastoris." Glycoconjugate Journal 36, no. 1 (2019): 27–38. http://dx.doi.org/10.1007/s10719-018-09855-x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!