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Dissertations / Theses on the topic 'Pili (Microbiology)'

Author: Grafiati

Published: 4 June 2021

Last updated: 20 February 2023

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1

Botten, James Alfons Desmond. "Role of sefD and sefR in the biogenesis of Salmonella enterica serovar Enteritidis SEF14 fimbriae." Title page, abstract and contents only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09phb7512.pdf.

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2

Kennouche, Paul. "New insights into meningococcal pathogenesis : exploring the role of the major pilin PilE in the functions of type IV pili Mechanisms of meningococcal type IV pili multiple functions revealed by deep mutational scanning." Thesis, Sorbonne Paris Cité, 2018. https://wo.app.u-paris.fr/cgi-bin/WebObjects/TheseWeb.woa/wa/show?t=1972&f=12515.

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Les pili de type IV (PT4) sont des filaments micrométriques qui exercent de multiples fonctions à la surface de nombreux procaryotes. Chez Neisseria meningitidis, les PT4 sont des homopolymères de la piline majeure PilE. Leur implication dans l'agrégation interbactérienne et l'adhésion aux cellules humaines les rend centraux dans la virulence du méningocoque. Cependant, les mécanismes permettant aux PT4 d'exercer ces diverses fonctions restent trop élusifs. Durant ce doctorat, nous avons simultanément déterminé les régions de PilE impliquées dans l'assemblage des pili, l'auto-agrégation, l'adh

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3

Paranjpye, Rohinee. "The role of a Vibrio vulnificus type IV pilin in pathogenesis and in persistence in oysters /." Thesis, Connect to this title online; UW restricted, 2005. http://hdl.handle.net/1773/5372.

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4

Choi, Suk Ho. "Binding mechanism of K88ab pili produced by enterotoxigenic Escherichia coli." Diss., Virginia Polytechnic Institute and State University, 1987. http://hdl.handle.net/10919/74764.

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Binding of K88ab pili by brush border membrane and mucus from pig small intestine was characterized by inhibition assay and Western blot. In Western blot, K88ab pili were bound by two major brush border membrane polypeptides with molecular weight of 61,500 and 57,000 in addition to numerous minor polypeptides and a major mucus polypeptide with molecular weight of 27,500. The results from Western blot assays with periodate oxidized and carbamylated brush border membrane and inhibition assay with brush border membrane glycopeptide suggest that amino groups (rather than carbohydrate) present on t

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5

Karlsson, Katarina Flemmer. "Synthesis, conformational analysis, and biological evaluation of peptides from E. coli P pilus proteins." Lund : Organic Chemistry 2, Lund Institute of Technology, Lund University, 1997. http://catalog.hathitrust.org/api/volumes/oclc/39777038.html.

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6

Charles-Orszag, Arthur. "Cellular and molecular mechanisms of human endothelial cell plasma membrane remodeling by Neisseria meningitidis." Thesis, Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCB045/document.

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Neisseria meningitidis est une bactérie diderme qui colonise le nasopharynx humain de façon commensale. Occasionnellement, elle franchit la barrière nasopharyngée et accède à la circulation sanguine où elle peut provoquer un choc septique et/ou une méningite Le pouvoir pathogène de N. meningitidis est lié à sa capacité à interagir avec les cellules endothéliales humaines. Après avoir adhéré aux cellules grâce à des organelles filamenteux, les pili de type IV, les bactéries induisent une déformation de la membrane plasmique de la cellule hôte sous la forme de protrusions riches en actine ressem

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7

Kuehn, Joanna Sue Clegg Steven. "Dam methylation and putative fimbriae in Klebsiella pneumoniae." Iowa City : University of Iowa, 2009. http://ir.uiowa.edu/etd/391.

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8

Warren, Matthew J. "Analysis of the role of phosphorylcholine in Neisseria meningitidis /." [St. Lucia, Qld.], 2006. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe19050.pdf.

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9

Choudhury, Devapriya. "Functional implications of macromolecular recognition : assembly of adhesive pili and enzyme substrate interactions /." Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 2001. http://epsilon.slu.se/avh/2001/91-576-5820-X.pdf.

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10

Wright, Denis Sebastian. "Cloning of the Bacteroides nodosus pilin gene and expression in Escherichia coli." Thesis, The University of Sydney, 1985. https://hdl.handle.net/2123/28530.

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The bacterium, Bagteroides nogosu , is the principal causative organism of footrot in sheep. a disease which causes major economic losses in Australia. Current footrot vaccines contain strains from each of the eight serogroups present in Australia. However, they are expensive and difficult to produce because B.nodosus an anaerobe has complex growth requirements and expression of the pilus, a major protective antigen is variable in liquid culture. The pilus (plural, pili) is composed essentially of one subunit protein called pilin and is the antigen which forms the basis for serotyping the org

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11

Lövkvist, Lena. "Receptor Interactions Between Pathogenic Bacteria and Host Cells." Doctoral thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7782.

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<p>This thesis focuses on host and pathogen specific interactions during invasive disease. We have investigated the role and impact of different virulence factors of <i>Neisseria gonorrhoeae, N. meningitidis</i> and <i>Streptococcus pyogenes</i> on host epithelial cells and <i>in vivo</i>. </p><p><i>N. gonorrhoeae</i> cause the sexually transmitted disease gonorrhoea and <i>N. meningitidis</i> is the most common cause of bacterial meningitis and may be leathal to the host within hours of infection. The neisserial type IV pili were shown to have an important impact on host cells for the induct

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12

Iredell, J. R. "Molecular export and pilin assembly : TCP biogenesis in Vibrio cholerae /." Title page, contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phi648.pdf.

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13

Harding, Christian Michael. "Discovery and demonstration of functional type IV pili production and post-translational modification by a medically relevant Acinetobacter species." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1428405412.

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14

Tomaras, Andrew P. "Genetic Determinants Required for Biofilm Formation by Acinetobacter baumannii." Miami University / OhioLINK, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=miami1102082749.

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15

Williams, Danielle A. "The AlgZ/R Two-Component System Is Responsible for Attenuation of Virulence in Pseudomonas aeruginosa." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etd/3340.

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Pseudomonas aeruginosa is an important opportunistic pathogen. Many P. aeruginosa virulence factors are regulated by the AlgZ/R two component system. AlgZ is the sensor histidine kinase which phosphorylates AlgR, the response regulator. AlgR activates transcription of different gene targets based upon its phosphorylation state. The genes that encode AlgZ and AlgR are transcribed in an operon. While regulation of algR expression has been well studied, regulation of algZ expression has not. Using a pilW mutant in concert with algZTF-lacZ transcriptional fusion, we conducted a transposon mutagene

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16

Danne, Camille. "Biosynthèse, rôles et régulation du pilus Pil1 chez Streptococcus gallolyticus." Paris 7, 2013. http://www.theses.fr/2013PA077108.

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Streptococcus gallolyticus est une bactérie à Gram positif qui colonise de façon asymptomatique le système gastrointestinal de 2,5 à 15 % de la population humaine. Cette bactérie est également responsable d'endocardites infectieuses et il existe une forte association entre une infection à S. Gallolyticus et le cancer du colon. La séquence du génome de la souche UCN34 révèle l'existence de trois loci pilus (pil1,pil2 et pil3). Nous avons caractérisé l’ operon pil1 et montré que le pilus Pil1 permet la colonisationet la formation de biofilm sur tissus exposant du collagène. Le rôle de Pill dans

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17

Hughes, Abigail. "PA2771 Affects algZ expression and AlgZ/R Phenotypic Outputs in Pseudomonas aeruginosa." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etd/3462.

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Pseudomonas aeruginosa is a central nosocomial pathogen that can infect any tissue in the human body. A two-component system in P. aeruginosa that regulates many virulence factors is the AlgZ/R system. A previously unidentified regulator of algZ, that does not affect algR, has been identified via transposon mutagenesis, ‘PA2771’. The mechanism of regulation has not been previously studied, and novel evidence of PA2771 functioning as a diguanyalate cyclase was observed. When PA2771 is active, cyclic di-GMP levels are high, promoting the upregulation of the fimU operon and Type VI pili. In the P

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18

Imhaus, Anne-Flore. "Rôle et mode d’action des pilines mineures des pili de type IV de Neisseria meningitidis." Thesis, Paris 5, 2013. http://www.theses.fr/2013PA05T019/document.

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Les pili de type IV (PT4), certainement les organelles les plus répandues des bactéries à Gram-négatif, sont des machineries à multiples fonctions qui jouent un rôle crucial dans la pathogenèse de nombreux pathogènes humains, notamment notre modèle Neisseria meningitidis. L’assemblage des PT4 nécessite une machinerie complexe incluant au moins vingt protéines localisées dans la membrane interne, le périplasme et la membrane externe. Certaines de ces protéines ne sont pas nécessaires pour la biosynthèse des PT4, mais supportent les fonctions qui leur sont associées. Ces protéines, appelées pili

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19

Paitier, Agathe. "Etude de la mise à l'échelle des piles à combustible microbiennes : collecteurs de courant et hydrodynamique." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSEI107/document.

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Répondre aux besoins énergétiques croissants de nos sociétés et limiter leur impact sur l’environnement est un enjeu actuel majeur. De nouvelles technologies alternatives comptent tirer profit de sources d’énergie négligées. Le potentiel énergétique des eaux usées peut être exploité par de nouvelles technologies telles que les piles à combustible microbiennes (PACM). Ces piles, pouvant produire de l’énergie électrique à partir d’eaux usées, montrent une diminution de leur rendement énergétique lorsque leur taille augmente, ce qui ne permet pas encore leur application industrielle. Ces travaux

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20

Basso, Pauline. "Exolysine, un facteur de virulence majeur de Pseudomonas aeruginosa." Thesis, Université Grenoble Alpes (ComUE), 2017. http://www.theses.fr/2017GREAV063/document.

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Pseudomonas aeruginosa est un pathogène opportuniste responsable d’infections nosocomiales sévères associées à un taux élevé de mortalité. Le système de sécrétion de Type III (SST3) et les effecteurs qu’il injecte sont considérés comme des facteurs de virulence prépondérants de P. aeruginosa. Récemment nous avons caractérisé, un groupe de souches ne possédant pas les gènes du SST3, mais dont la virulence repose sur la sécrétion d’une nouvelle toxine de 172 kDa, nommée Exolysine (ExlA) qui provoque la perméabilisation de la membrane des cellules hôtes. ExlA est sécrétée dans le milieu par une p

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21

Novotny, Laura Anne. "Noninvasive immunization strategies to target dendritic cells and protect against experimental otitis media due to nontypeable Haemophilus influenzae." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1299190518.

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22

Horzempa, Joseph. "Pseudomonas aeruginosa 1244 pilin glycosylation substrate specificity, glycan functionality, and application for vaccine development /." 2006. http://etd1.library.duq.edu/theses/available/etd-07172006-144355/.

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23

Porsch, Eric Allen. "Insights Into the Virulence Determinants of the Emerging Pathogen Kingella kingae." Diss., 2012. http://hdl.handle.net/10161/5860.

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<p><italic>Kingella kingae</italic> is an emerging bacterial pathogen that is being recognized increasingly as an important etiology of septic arthritis, osteomyelitis, and bacteremia, especially in young children. The pathogenesis of <italic>K. kingae</italic> disease begins with bacterial adherence to respiratory epithelium in the posterior pharynx. Previous work identified type IV pili as a critical factor for adherence to human epithelial cells. However, the finding that a significant percentage of pharyngeal isolates are non-piliated suggests that <italic>K. kingae</italic> expresses a

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24

Greenwood, John Milton. "The production and characterization of monoclonal antibodies against K88 pili from porcine enterotoxigenic Escherichia coli." 1985. http://hdl.handle.net/2097/27447.

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25

Aagesen, Alisha M. "Investigating Vibrio parahaemolyticus interactions with the Pacific oyster, Crassostrea gigas." Thesis, 2012. http://hdl.handle.net/1957/35769.

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Vibrio parahaemolyticus is a Gram-negative, halophilic, human pathogenic bacterium ubiquitous in the marine environment. Like many Vibrio species, V. parahaemolyticus commonly associates with shellfish, particularly oysters. Ingestion of a raw or under cooked oysters contaminated with V. parahaemolyticus can cause gastroenteritis, which is typically self-limiting and rarely causes death. Globally, oyster production is highly lucrative, especially on the West Coast of the United States where approximately 60% of oyster production occurs each year. Outbreaks of V. parahaemolyticus can result

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26

David, Élise. "Caractérisation et délétion de tous les systèmes d'adhésion connus de Salmonella enterica sérovar Typhi." Thèse, 2012. http://hdl.handle.net/1866/8716.

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Les fimbriae sont des structures protéiques extracellulaires retrouvées chez une vaste diversité de bactéries. Ces structures ont fait l’objet de nombreuses études et sont maintenant reconnus pour leur implication dans l’adhésion et l’invasion aux cellules eucaryotes, mais aussi dans la production de biofilms. Ils sont groupés selon leur voie de sécrétion. Certains utilisent une machinerie spécifique et individuelle, c’est le cas des pili de type IV, tandis que d’autres utilisent la voie de sécrétion générale suivit d’une voie spécifique telle que la voie du chaperon-placier (« Chaperon Usher

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27

(8771495), Layla Ramos-Hegazy. "Biofilm and Virulence Regulation in the Cystic Fibrosis-Associated Pathogens, Stenotrophomonas maltophilia and Pseudomonas aeruginosa." Thesis, 2020.

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Cystic fibrosis (CF) is a fatal, incurable genetic disease that affects over 30,000 people in the United States alone. People with this disease have a homozygous mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) which causes defects in chloride transport and leads to build up of mucus in the lungs and disruption of function in various organs. CF patients often suffer from chronic bacterial infections within the lungs, wherein the bacteria persist as a biofilm, leading to poor prognosis. Two of these pathogens, <i>Stenotrophomonas maltophilia</i> and <i>Pseudomonas aeru

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28

Abdelmadjid, Imen. "Fonction de l'AmtB dans la régulation de la nitrogénase chez Rhodobacter capsulatus." Thèse, 2010. http://hdl.handle.net/1866/3876.

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La fixation de l’azote diatomique est un processus très important à la vie, vu sa nécessité dans la biosynthèse de plusieurs molécules de base; acides aminés, acides nucléiques, etc. La réduction de l’azote en ammoniaque est catalysée par la nitrogénase, une enzyme consommatrice de beaucoup d’énergie étant donné qu’elle nécessite 20 à 30 moles d’ATP pour la réduction d’une mole d’azote. De ce fait une régulation rigoureuse est exigée afin de minimiser le gaspillage d’énergie. Plusieurs systèmes de contrôle sont connus, aussi bien au niveau post-traductionnel que traductionnel. Chez la ba

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29

Richter, Lubna V. "Mutational analysis of geopilin function in Geobacter sulfurreducens." 2011. https://scholarworks.umass.edu/dissertations/AAI3465075.

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Geobacter sulfurreducens possesses type IV pili that are considered to be conductive nanowires and a crucial structural element in biofilm formation, enabling electron transfer to insoluble metal oxides in anaerobic sediments and to graphite anodes in microbial fuel cells. The molecular mechanism by which electrons are transferred through the nanowires to the electron acceptor is not fully understood. Prior to the work described in this thesis, the gene (pilA) encoding the structural pilus subunit had been identified, but little was known about the functional translation start codon, the lengt

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30

Grüning, Maren Marine. "Effects of insect mass outbreaks on the C and N balance in forest ecosystems." Doctoral thesis, 2019. http://hdl.handle.net/21.11130/00-1735-0000-0003-C143-7.

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