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1

Sun, Tingyu, Yahui Wang, Xiaoqin Wu, Yang Wang, Aixia Yang, and Jianren Ye. "Pinus thunbergii Parl. Somatic Plants’ Resistance to Bursaphelenchus xylophilus Depends on Pathogen-Induced Differential Transcriptomic Responses." International Journal of Molecular Sciences 25, no. 10 (2024): 5156. http://dx.doi.org/10.3390/ijms25105156.

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Pinus thunbergii Parl. is an economically and medicinally important plant, as well as a world-renowned horticultural species of the Pinus genus. Pine wilt disease is a dangerous condition that affects P. thunbergii. However, understanding of the genetics underlying resistance to this disease is poor. Our findings reveal that P. thunbergii’s resistance mechanism is based on differential transcriptome responses generated by the early presence of the pathogen Bursaphelenchus xylophilus, also known as the pine wood nematode. A transcriptome analysis (RNA-seq) was performed to examine gene expressi
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2

Saunders, Rachel. "Planting Edo: Pinus thunbergii." Arnoldia 78, no. 3 (2021): 44–45. http://dx.doi.org/10.5962/p.364391.

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3

Zhang, Yu, Guicai Du, Qunqun Guo, et al. "Transcriptome Sequencing and Analysis of Genes Related to Disease Resistance in Pinus thunbergii." Forests 14, no. 3 (2023): 650. http://dx.doi.org/10.3390/f14030650.

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Pinus thunbergii (P. thunbergii) is a gymnosperm with important economic and ecological value. In order to investigate the diagnosis and defense mechanism of P. thunbergii against Bursaphelenchus xylophilus (the pinewood nematode, PWN), the needles of P. thunbergii seedlings on the fifth day after being infected by PWN were taken as samples for transcriptome sequencing analysis. Compared with the control group, 647 genes were differentially expressed in the treatment group, of which 277 genes were upregulated and 370 genes were downregulated. Enrichment analysis showed that most of these diffe
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4

Ji, Yingchao, Chenyu Song, Long Chen, Xue Zheng, Chunyan Jia, and Yanxue Liu. "Interspecific Relationship Between Monochamus alternatus Hope and Arhopalus rusticus (L.) in Pinus thunbergii Affected by Pine Wilt Disease." Forests 15, no. 11 (2024): 2037. http://dx.doi.org/10.3390/f15112037.

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Monochamus alternatus Hope and Arhopalus rusticus (L.) are important stem-boring pests that co-occur on weakened Pinus spp. Their larvae damage the xylem and phloem of the trunks and branches. At present, the consequences of the interspecific relationship between two longicorn beetles on the same host of Pinus trees are unclear. The population dynamics and spatial distribution of these two species on Pinus thunbergii trees were investigated to clarify the ecological niches and interspecific relationship of two longicorn beetles on the different degrees of decline in P. thunbergii trees. The re
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5

Zhang, Xiaorui, Zhuo Liu, Mu Cao, and Tingting Dai. "Effects of Thinning of the Infected Trees and Cultivating of the Resistant Pines on Soil Microbial Diversity and Function." Forests 16, no. 5 (2025): 813. https://doi.org/10.3390/f16050813.

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Pine wilt disease (PWD) poses a significant threat to pine forest health, making sanitation thinning of infected trees and cultivation of disease-resistant pine stands crucial measures for forest ecosystem restoration. To date, limited studies have systematically investigated how post-sanitation planting of pine-wilt-disease-resistant Pinus species affects soil microbiome, especially regarding bacterial and fungal diversity characteristics, functional succession patterns, and community assembly processes. In this study, we performed a comparative analysis of soil microbial community characteri
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6

Wang, Xin-Yu, Xiao-Qin Wu, Tong-Yue Wen, Ya-Qi Feng, and Yan Zhang. "Terpene Production Varies in Pinus thunbergii Parl. with Different Levels of Resistance, with Potential Effects on Pinewood Nematode Behavior." Forests 13, no. 7 (2022): 1140. http://dx.doi.org/10.3390/f13071140.

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Determining the mechanisms of pine wilt disease (PWD) resistance in Pinus is a popular research topic, but information on volatile organic substances (VOS) and their role in PWD is lacking. Whether the difference in VOS among Pinus thunbergii parl. that have different levels of resistance with pine wood nematodes (PWNs) is the reason for the differing resistance needs to be studied. In this study, resistant P. thunbergii introduced from Japan and susceptible P. thunbergii native to China were used to investigate the effects of different lines inoculated with PWN. We determined the expression l
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7

Xue, Qi, Yang Xiang, Xiao-Qin Wu, and Ming-Jie Li. "Bacterial Communities and Virulence Associated with Pine Wood Nematode Bursaphelenchus xylophilus from Different Pinus spp." International Journal of Molecular Sciences 20, no. 13 (2019): 3342. http://dx.doi.org/10.3390/ijms20133342.

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Bursaphelenchus xylophilus, the causal agent of pine wilt disease, is a destructive threat to pine forests. The role of bacteria associated with B. xylophilus in pine wilt disease has attracted widespread attention. This study investigated variation in bacterial communities and the virulence of surface-sterilized B. xylophilus from different Pinus spp. The predominant culturable bacteria of nematodes from different pines were Stenotrophomonas and Pseudomonas. Biolog EcoPlate analysis showed that metabolic diversity of bacteria in B. xylophilus from P. massoniana was the highest, followed by P.
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8

Maehara, Noritoshi, Junichi Kikuchi, and Kazuyoshi Futai. "Mycorrhizae of Japanese black pine (Pinus thunbergii): protection of seedlings from acid mist and effect of acid mist on mycorrhiza formation." Canadian Journal of Botany 71, no. 12 (1993): 1562–67. http://dx.doi.org/10.1139/b93-190.

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To determine the effects of acid precipitation on Japanese black pine (Pinus thunbergii Parl.) with and without mycorrhizae (Pisolithus tinctorius (Pers.) Coker et Couch), 1-year-old seedlings were exposed to simulated acid mist, pH 3.0, for 10 min per day twice a week for 3 or 4 months. Simulated acid mist adversely affected the transpiration rate and lowered the extractable phosphorus content of seedlings, but seedlings with mycorrhizae were less affected by acid mist than were nonmycorrhizal seedlings. Simulated acid mist also retarded mycorrhiza formation. Key words: mycorrhizae, phosphoru
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9

Mukasyaf, Aziz Akbar, Koji Matsunaga, Miho Tamura, Taiichi Iki, Atsushi Watanabe, and Masakazu G. Iwaizumi. "Reforestation or Genetic Disturbance: A Case Study of Pinus thunbergii in the Iki-no-Matsubara Coastal Forest (Japan)." Forests 12, no. 1 (2021): 72. http://dx.doi.org/10.3390/f12010072.

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In the twentieth century, a substantial decline in Pinus thunbergii populations in Japan occurred due to the outbreak of pine wood nematode (PWN), Burshaphelencus xylophilus. A PWN-P. thunbergii resistant trees-breeding project was developed in the 1980s to provide reforestation materials to minimalize the pest damage within the population. Since climate change can also contribute to PWN outbreaks, an intensive reforestation plan instated without much consideration can impact on the genetic diversity of P. thunbergii populations. The usage and deployment of PWN-P. thunbergii resistant trees to
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10

Mukasyaf, Aziz Akbar, Koji Matsunaga, Miho Tamura, Taiichi Iki, Atsushi Watanabe, and Masakazu G. Iwaizumi. "Reforestation or Genetic Disturbance: A Case Study of Pinus thunbergii in the Iki-no-Mastubara Coastal Forest (Japan)." Forests 12, no. 1 (2021): 72. http://dx.doi.org/10.3390/f12010072.

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In the twentieth century, a substantial decline in Pinus thunbergii populations in Japan occurred due to the outbreak of pine wood nematode (PWN), Burshaphelencus xylophilus. A PWN-P. thunbergii resistant trees-breeding project was developed in the 1980s to provide reforestation materials to minimalize the pest damage within the population. Since climate change can also contribute to PWN outbreaks, an intensive reforestation plan instated without much consideration can impact on the genetic diversity of P. thunbergii populations. The usage and deployment of PWN-P. thunbergii resistant trees to
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11

Hata, Kunihiko, Kazuyoshi Futai, and Mitsuya Tsuda. "Seasonal and needle age-dependent changes of the endophytic mycobiota in Pinus thunbergii and Pinus densiflora needles." Canadian Journal of Botany 76, no. 2 (1998): 245–50. http://dx.doi.org/10.1139/b97-177.

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Monthly changes in the endophytic mycobiota in Pinus thunbergii Parl. and Pinus densiflora Sieb. et Zucc. needles were detected by tissue culture. Endophytic mycobiota of these pine needles were dominated by four taxa: the Leptostroma anamorph of the Lophodermium pinastri (Schrad. ex Hook.) Chev. complex, Phialocephala sp., Cenangium ferruginosum Fr.:Fr., and an unidentified hyphomycete species (BrS). The mycobiota differed with host pine species, position on the needle, and needle age. Virtually no endophytes were detected in needles just after emergence, but most segments of the older needle
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12

Mukasyaf, Aziz Akbar. "BIOGEOGRAPHIC PATTERNS OF GENETIC DIVERSITY OF Pinus thunbergii POPULATIONS ACROSS JAPAN." Nativa 13, no. 1 (2025): 1015–113. https://doi.org/10.31413/nat.v13i1.18514.

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This study examines the genetic diversity of Pinus thunbergii populations across various biogeographic regions in Japan, with a focus on the influence of geographic and environmental factors on genetic variation. Using seven EST-SSR markers, the objective was to analyze the influence of geographic factors, gene flow, and historical events on population structure. The research also compared the utility of EST-SSR markers with six genomic SSR (G-SSR) markers, revealing significant differences between them. EST-SSR markers showed lower genetic diversity compared to G-SSR, which could indicate hig
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13

Qiao, Changxia, Ruiwen Zhao, Dewei Li, and Xiaolei Ding. "A New Species of Biscogniauxia Associated with Pine Needle Blight on Pinus thunbergii in China." Forests 15, no. 6 (2024): 956. http://dx.doi.org/10.3390/f15060956.

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In June 2020, needle blight symptoms on Pinus thunbergii were discovered in Bazhong City, Sichuan Province, China. Fungal isolates were obtained from the pine needles of P. thunbergii. After examining morphological characteristics and conducting multi-locus (ITS, ACT, TUB2 and RPB2) phylogenetic analyses, the isolates SC1–SC5 were determined to be a new species, Biscogniauxia sinensis. Genealogical Concordance Phylogenetic Species Recognition with a pairwise homoplasy index test was used to further verify the results of the phylogenetic analyses. The morphology and phylogenetic relationships b
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14

Hata, Kunihiko, and Kazuyoshi Futai. "Endophytic fungi associated with healthy pine needles and needles infested by the pine needle gall midge, Thecodiplosis japonensis." Canadian Journal of Botany 73, no. 3 (1995): 384–90. http://dx.doi.org/10.1139/b95-040.

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The endophytic mycobiota of the galls of pine needle gall midge, Thecodiplosis japonensis, and healthy current-year needles of Pinus densiflora and the F2 hybrid pine (Pinus thunbergii × (P. thunbergii × P. densiflora)) was compared. Phialocephala sp. was the dominant endophyte isolated from the galls and from the basal regions of healthy needles. Species richness in endophytes was facilitated in galls compared to the basal region of healthy needles, and the commencement of their colonization was hastened in galls. In the early stages of gall formation, however, no endophyte was isolated, sugg
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15

Kong, Baishu, Jilei Zhou, Liguo Qi, et al. "Effects of Nitrogen Deposition on Leaf Litter Decomposition and Soil Organic Carbon Density in Arid and Barren Rocky Mountainous Regions: A Case Study of Yimeng Mountain." Forests 14, no. 7 (2023): 1351. http://dx.doi.org/10.3390/f14071351.

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The ecological impact of nitrogen (N) deposition has gained significance since the advent of the industrial revolution. Although numerous studies have examined the impact of N deposition on soil organic carbon (SOC), certain arid and barren rocky mountainous regions, which experience more pronounced N limitations, have been overlooked. This study was conducted in the Yimeng Mountains, examining eight treatments created by four N addition levels (0 kg N ha−1 yr−1, 50 kg N ha−1 yr−1, 100 kg N ha−1 yr−1 and 200 kg N ha−1 yr−1) and two tree species (Quercus acutissima Carruth. and Pinus thunbergii
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16

Sun, Tingyu, Mati Ur Rahman, Xiaoqin Wu, and Jianren Ye. "Resistant and Susceptible Pinus thunbergii ParL. Show Highly Divergent Patterns of Differentially Expressed Genes during the Process of Infection by Bursaphelenchus xylophilus." International Journal of Molecular Sciences 24, no. 18 (2023): 14376. http://dx.doi.org/10.3390/ijms241814376.

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Pine wilt disease (PWD) is a devastating disease that threatens pine forests worldwide, and breeding resistant pines is an important management strategy used to reduce its impact. A batch of resistant seeds of P. thunbergii was introduced from Japan. Based on the resistant materials, we obtained somatic plants through somatic embryogenesis. In this study, we performed transcriptome analysis to further understand the defense response of resistant somatic plants of P. thunbergii to PWD. The results showed that, after pine wood nematode (PWN) infection, resistant P. thunbergii stimulated more dif
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17

Zhang, Xuejiao, Shuailong Wang, Quan Zhou, et al. "Phenotypic Changes in Pinus thunbergii, Larix kaempferi, Picea koraiensis, and Abies holophylla Seedlings Inoculated with Pine Wilt Nematode: Revealing the Resistance." Forests 16, no. 1 (2025): 137. https://doi.org/10.3390/f16010137.

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Bursaphelenchus xylophilus (pine wood nematode, PWN) has been present in China for over 40 years and has spread to northeast China, where native pine species are key components of the local top community. Pinus thunbergii is known to be susceptible to PWN among local conifer species, whereas research on PWN’s pathogenicity in Larix remains limited. Furthermore, there are no research reports on PWN infestation in Picea and Abies species within China. This study conducted a detailed analysis of phenotypic changes and temporal spectral reflectance variations in four conifer species in northeast C
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18

Haraguchi, Akira, and Masato Sakaki. "Formation of Soil Chemical Environment in Coastal Pinus thunbergii Parlatore Forest in Southwestern Japan." Water 12, no. 6 (2020): 1544. http://dx.doi.org/10.3390/w12061544.

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We investigated the chemical properties of precipitation and litter fall, and their effects on soil chemistry, in a coastal forest consisting of pure Pinus thunbergii stands, Pinus-dominated stands with broadleaf trees in the understory, mixed stands of Pinus and evergreen broadleaf trees, and evergreen broadleaf stands. Throughfall pH in the pure Pinus stand was significantly lower than those in the other three stands, and the soil in the pure Pinus stand was determined to be acidic (pH = ca. 5.0). In Pinus-dominated stands with broadleaf species in the understory, precipitation had a neutral
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19

simini, Michael, and Ida A. Leone. "The Role of Alkanes in Epicuticular Wax Relative to Tolerance of Pine Species to Saline Spray." Forest Science 32, no. 2 (1986): 487–92. http://dx.doi.org/10.1093/forestscience/32.2.487.

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Abstract A study of the epicuticular waxes on the foliage of saline-spray tolerant Japanese black pine (Pinus thunbergii) and salt-sensitive eastern white pine (Pinus strobus) showed a greater percentage of longer chain alkanes in the former species (87 percent) than in the latter (68 percent). Alkane concentrations increased with increasing temperature and longer photoperiod. The longer chain alkanes are reportedly more resistant to the penetration of polar solutions than are shorter chain alkanes. Alkane concentration was greater for both pine species in trees grown at higher vs lower temper
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20

Sono, Masakazu, Hitomi Yamaguchi, Katsuyuki Nakashima, and Motoo Tori. "A New Diterpenoid and a Lignan from Pinus thunbergii." Natural Product Communications 12, no. 8 (2017): 1934578X1701200. http://dx.doi.org/10.1177/1934578x1701200808.

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Phytochemical analysis of Pinus thunbergii Parl. afforded eleven compounds, two of which were new. One was 8β,12ξ-dihydroxylabd-13( E)-en-19-oic acid and the other was a lignan acetate. The NMR data of all isolated compounds were recorded.
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21

Townsend, A. M., and W. F. Kwolek. "Relative Susceptibility of Thirteen Pine Species to Sodium Chloride Spray." Arboriculture & Urban Forestry 13, no. 9 (1987): 225–28. http://dx.doi.org/10.48044/jauf.1987.047.

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Seedlings of 13 pine species were sprayed with either NaCI spray or distilled water for 3 consecutive winters. Pinus thunbergii, P. ponderosa, and Pinus nigra were generally most tolerant, with the least foliar injury, best survival, and least growth reduction. Pinus strobus, P. bankslana, P. cembra, P. peuce, and P. densiflora were most susceptible. Species intermediate in susceptibility were P. strobiformis, P. aristata, P. parviflora, P. reslnosa, and P. sylvestris. Species with the lowest internal sodium and chloride levels after treatment generally were most tolerant to the salt spray.
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22

Menéndez-Gutiérrez, María, Koji Matsunaga, and Katsumi Togashi. "Relationship between pine wilt-tolerance rankings of Pinus thunbergii trees and the number of Bursaphelenchus xylophilus passing through branch sections." Nematology 19, no. 9 (2017): 1083–93. http://dx.doi.org/10.1163/15685411-00003108.

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To establish a simple, time-saving method for selecting Pinus tree candidates tolerant to the infection of Bursaphelenchus xylophilus, the causative agent of pine wilt disease, three experiments were conducted. When two virulent isolates Sc-9 and T-4 were inoculated on cut ends of branch sections, more nematodes were inhibited from passing through P. taeda branch sections than P. thunbergii sections. Sc-9 tended to pass through P. thunbergii sections more easily than T-4. The probability of nematodes passing through 5-cm-long P. thunbergii branch sections was greater when 200 nematodes were in
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23

Lim, Dong Ok, and Woong Young Soh. "Cambial Development and Tracheid Length of Dwarf Pines (Pinus Densiflora and P. Thunbergii)." IAWA Journal 18, no. 3 (1997): 301–10. http://dx.doi.org/10.1163/22941932-90001495.

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From a comparison of cambial cells and their derivatives between naturally occurring dwarf trees and normal ones, it was concluded that tracheids in the annual rings of dwarf trees are shorter, narrower and fewer than those of normal trees. The frequency of anticlinal division and loss of cambial initials is low during differentiation of xylem cells from cambial initials in dwarf pines. The length and intrusive growth of fusiform initials are slightly less than those of normal trees. Thus, it is concluded that the shortening of tracheids in dwarf trees is due to the fact that cambial initials
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24

Chu, Xu, Qiuyu Ma, Meijiao Yang, et al. "Diversity and Distribution of Xylophagous Beetles from Pinus thunbergii Parl. and Pinus massoniana Lamb. Infected by Pine Wood Nematode." Forests 12, no. 11 (2021): 1549. http://dx.doi.org/10.3390/f12111549.

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The vectors of pinewood nematode of Bursaphelenchus xylophilus (Steiner & Bührer, 1934) are mainly known as xylophagous beetles. Understanding the composition and distribution of these xylophagous beetles in host pine trees infected by PWN is critical to control the spread of PWN. In this study, we investigated the community structures of the xylophagous beetles in two main host trees in Fujian and Shandong, Pinus massoniana Lamb. and Pinus thunbergia Parl., in different stages of infection. All beetles were collected by dissecting the whole pine trees and then identified by their morpholo
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25

Chae, Hee Myung, Sung Hwan Choi, Sang Hoon Lee, Sangsub Cha, Keum Chul Yang, and Jae Kuk Shim. "Effect of Litter Quality on Needle Decomposition for Four Pine Species in Korea." Forests 10, no. 5 (2019): 371. http://dx.doi.org/10.3390/f10050371.

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Litter decomposition involves multiple complex processes, including interactions between the physicochemical characteristics of litter species and various environmental factors. We selected four representative pine species in South Korea (Pinus densiflora Siebold & Zucc., Pinus thunbergii Parl., Pinus koraiensis Siebold & Zucc., and Pinus rigida Miller) to investigate the decay rate and effects of the physicochemical properties on decomposition. Needle litters were incubated in microcosms at 23 °C for 280 days and retrieved four times in about 70-day intervals. The mass loss showed sig
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26

Wen, Tong-Yue, Yan Zhang, Xiao-Qin Wu, Jian-Ren Ye, Yi-Jun Qiu, and Lin Rui. "Studies on the Requirement of Transthyretin Protein (BxTTR-52) for the Suppression of Host Innate Immunity in Bursaphelenchus xylophilus." International Journal of Molecular Sciences 23, no. 23 (2022): 15058. http://dx.doi.org/10.3390/ijms232315058.

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The pinewood nematode, Bursaphelenchus xylophilus, has been determined as one of the world’s top ten plant-parasitic nematodes. It causes pine wilt, a progressive disease that affects the economy and ecologically sustainable development in East Asia. B. xylophilus secretes pathogenic proteins into host plant tissues to promote infection. However, little is known about the interaction between B. xylophilus and pines. Previous studies reported transthyretin proteins in some species and their strong correlation with immune evasion, which has also been poorly studied in B. xylophilus. In this stud
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27

Shpatov, Alexander V., Sergey A. Popov, Olga I. Salnikova, Ekaterina A. Khokhrina, Emma N. Shmidt, and Byung Hun Um. "Low-Volatile Lipophilic Compounds in Needles, Defoliated Twigs, and Outer Bark of Pinus thunbergii." Natural Product Communications 8, no. 12 (2013): 1934578X1300801. http://dx.doi.org/10.1177/1934578x1300801227.

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Despite a long history of the use of Pinus thunbergii for technical, medicinal, agricultural, and other purposes, the composition of low-volatile metabolites in the used parts of the plant has been poorly investigated. We report here on the distribution of lipophilic extractive compounds in different parts of the shoot system (needles, defoliated twigs, outer bark) of P. thunbergii studied by GC/MS. The highest and lowest contents of lipophilic substances were found in defoliated twigs and in outer bark correspondingly. Acid compounds in the extract of needles comprised mainly labdane type dit
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Park, Gildong, Daesung Lee, Yeongwan Seo, and Jungkee Choi. "Height Growth Models for Pinus thunbergii in Jeju Island." Journal of Forest and Environmental Science 31, no. 4 (2015): 255–60. http://dx.doi.org/10.7747/jfes.2015.31.4.255.

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29

Kimura, Makoto. "Seasonal change in specific needle weight of Pinus thunbergii." Ecological Research 7, no. 2 (1992): 199–202. http://dx.doi.org/10.1007/bf02348501.

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30

Kim, Chang-Kyun, Ju-Kyeong Eo, and Ahn-Heum Eom. "Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Pinus thunbergii." Korean Journal of Mycology 40, no. 4 (2012): 183–86. http://dx.doi.org/10.4489/kjm.2012.40.4.183.

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31

Mukasyaf, Aziz Akbar. "Biogeography: A Case in Spatial Distribution Pattern of 21 Pinus thunbergii Populations in Japan." IOP Conference Series: Earth and Environmental Science 1357, no. 1 (2024): 012002. http://dx.doi.org/10.1088/1755-1315/1357/1/012002.

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Abstract Biogeography is study of spatial distribution of organisms that has always been a source of fascination for naturalists, ecologists, and scientists. Evaluating genetic differentiation at various spatial scales provides valuable biogeographical insights relate to genetic structure of the species within the populations. The Expressed Sequence Tag - Simple Sequence Repeat, EST-SSR, marker used in these studies to gain insights into the efficiency of this marker. The objective of these studies was to provide a deeper understanding of the genetic diversity, structure, and phylogeographic h
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32

Sun, Tingyu, Yanli Wang, Lihua Zhu, Xiaoqin Wu, and Jianren Ye. "Plant regeneration by somatic embryogenesis in Pinus thunbergii resistant to the pine wood nematode." Canadian Journal of Forest Research 49, no. 12 (2019): 1604–12. http://dx.doi.org/10.1139/cjfr-2018-0522.

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Pine wilt disease (PWD) is a severe threat to pine forests in East Asia. Screening and breeding of resistant varieties is a very effective way to prevent and control PWD; however, no reliable somatic embryogenesis system has yet been developed for the elite nematode-resistant Pinus thunbergii Parl. line. In this study, we studied the plant regeneration via somatic embryogenesis of nematode-resistant P. thunbergii. Initiation of embryogenic tissue was significantly affected by seed family (p = 0.017), immature zygotic embryo stage (p = 0.032), and initiation medium (p = 0.004). Seed family 37 w
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33

Kitagami, Yudai, Natsumi Kanzaki, Toko Tanikawa, and Yosuke Matsuda. "Free-living nematodes associated with pine cones of Pinus thunbergii and P. taeda at Japanese coastal and inland forest sites." Nematology 21, no. 4 (2019): 389–400. http://dx.doi.org/10.1163/15685411-00003221.

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Summary We surveyed the distribution of nematodes in 56 cones of Pinus thunbergii collected from both live branches and on the forest floor in three coastal and inland habitats and in 11 cones of P. taeda collected at different heights. We identified 47 nematodes to family or genera by analysis of an 18S small subunit rRNA gene sequence. The frequencies of occurrence of free-living cone nematodes were 97% in coastal P. thunbergii, 92% in inland P. thunbergii, and 82% in P. taeda. Phylogenetic analysis assigned the nematodes to four clades with high bootstrap values. Nine sequences that were fo
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34

Narita, Hiroe, Junji Sugiyama, and Shigenori Kuga. "Chemotaxonomical identification of Holocenic bogwood recovered after 2007 Niigataken Chuestsu-oki Earthquake." Holzforschung 66, no. 8 (2012): 951–57. http://dx.doi.org/10.1515/hf-2011-0149.

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Abstract A Holocenic coniferous bogwood salvaged from the 70 to 100 m-deep seabed after the 2007 Niigataken Chuetsu-oki Earthquake was analyzed anatomically and chemically. The main anatomical features of the wood were the presence of (i) large window-like pits in cross field and (ii) smooth-walled cells of ray tracheid. Feature (i) allowed identification of the wood as genus Pinus. Feature (ii) is characteristic of section Quinquefoliae, but possible decay by the long burial (3400–3210 cal BP) left the identification inconclusive. Chemical analysis showed that the major volatile components of
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35

Rim, Soon Ok, Mehwish Roy, Junhyun Jeon, Jake Adolf V. Montecillo, Soo-Chul Park, and Hanhong Bae. "Diversity and Communities of Fungal Endophytes from Four Pinus Species in Korea." Forests 12, no. 3 (2021): 302. http://dx.doi.org/10.3390/f12030302.

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Fungal endophytes are ubiquitous in nature. They are known as potential sources of natural products, and possible agents for biocontrol attributing to their ability to produce a repertoire of bioactive compounds. In this study, we isolated fungal endophytes from three different tissues (needle, stem and root) of four Pinus species (Pinus densiflora, Pinus koraiensis, Pnus rigida, and Pinus thunbergii) across 18 sampling sites in Korea. A total number of 5872 culturable fungal endophytes were isolated using standard culturing techniques. Molecular identification based on the sequence analyses o
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Gudžinskas, Zigmantas, Lukas Petrulaitis, and Egidijus Žalneravičius. "New woody alien plant species recorded in Lithuania." Botanica Lithuanica 23, no. 2 (2017): 153–68. http://dx.doi.org/10.1515/botlit-2017-0017.

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AbstractMany woody plant species that originate from various regions of the world have been introduced in other regions or continents and are used in ornamental gardening, silviculture, erosion control, for fruit sources or other purposes. Woody plants selected for introduction usually originate from regions with similar climate conditions; therefore, after certain time lag they start to spread outside places of cultivation, become naturalized or even invasive. In addition to 77 woody alien plant species reported in Lithuania, ten new species were recorded and analysed in this paper. Informati
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Suharyanto and Susumu Shiraishi. "Nucleotide Diversities and Genetic Relationship in the Three Japanese Pine Species; Pinus thunbergii, Pinus densiflora, and Pinus luchuensis." Diversity 3, no. 1 (2011): 121–35. http://dx.doi.org/10.3390/d3010121.

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Tsukahara, H., T. T. Kozlowski, and J. Shanklin. "Tolerance ofPinus densiflora, Pinus thunbergii, andLarix leptolepis seedlings to SO2." Plant and Soil 88, no. 3 (1985): 385–97. http://dx.doi.org/10.1007/bf02197495.

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Won, Sang-Jae, Vantha Choub, Jun-Hyeok Kwon, Dong-Hyun Kim, and Young-Sang Ahn. "The Control of Fusarium Root Rot and Development of Coastal Pine (Pinus thunbergii Parl.) Seedlings in a Container Nursery by Use of Bacillus licheniformis MH48." Forests 10, no. 1 (2018): 6. http://dx.doi.org/10.3390/f10010006.

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This study investigated the control of Fusarium root rot and development of coastal pine (Pinus thunbergii) seedlings in a container nursery by using Bacillus licheniformis MH48. High-quality seedlings without infectious diseases cause vigorous growth. Fusarium root rot caused by Fusarium oxysporum is responsible for serious damage to coastal pine seedlings in nurseries. B. licheniformis MH48 produced enzymes that degraded the fungal cell walls, such as chitinase and β-1,3-glucanase. These lytic enzymes exhibited destructive activity toward F. oxysporum hyphae, which were found to play key rol
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Jo, Sangjin, Dong-Keun Yi, and Ki-Joong Kim. "Diurnal hawk moth pollination of Melampyrum koreanum (Orobanchaceae) and the origin of this endemic Korean species." Phytotaxa 172, no. 1 (2014): 31. http://dx.doi.org/10.11646/phytotaxa.172.1.4.

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Melampyrum koreanum (Orobanchaceae) was described recently as an endemic species in Korea. This study examined the distribution range, relationship with the host plant, flower developmental pattern, pollinator, pollination mechanism, and seed development of M. koreanum (Orobanchaceae) on the island of Somaemul-do, Korea. M. koreanum is hemiparasitic and a strong candidate for its host plant is Pinus thunbergii, with which it is distributed sympatrically. The flower of M. koreanum represents a typical hawk moth pollination syndrome. Macroglossum pyrrhostictum Butler (Sphingidae, Lepidoptera), a
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HIZUME, Masahiro, Fumio ISHIDA, and Minoru MURATA. "Multiple locations of the rRNA genes in chromosomes of pines, Pinus densiflora and P. thunbergii." Japanese Journal of Genetics 67, no. 5 (1992): 389–96. http://dx.doi.org/10.1266/jjg.67.389.

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Shimomura, Norihiro, Miyuki Matsuda, Kunio Ariyoshi, and Tadanori Aimi. "Development of mycelial slurries containing surfactant for cultivation of the edible ectomycorrhizal mushroom Rhizopogon roseolus (syn. Rhizopogon rubescens)." Botany 90, no. 9 (2012): 839–44. http://dx.doi.org/10.1139/b2012-054.

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Rhizopogon roseolus (Corda) Th. M. Fr., known as shoro in Japanese, is an edible ectomycorrhizal mushroom. To successfully cultivate ectomycorrhizal mushrooms, inoculation and propagation methods must be developed, and isolates with superior traits must be selected. Pinus thunbergii Parl. seedlings were inoculated with several isolates of R. roseolus using the Petri dish technique and their mycorrhizal abilities were assessed. We selected a superior isolate that rapidly colonized and produced a lot of ectomycorrhizas in the roots of P. thunbergii. The selected strain was cultured in liquid med
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Mao, Peili, Hongxiang Mu, Banghua Cao, Yuhong Liu, Zhaofei Fan, and Shumei Wang. "Effects of sand burial and overstory tree age on seedling establishment in coastal Pinus thunbergii forests in the northern Shandong Peninsula, China." Forestry Chronicle 92, no. 03 (2016): 357–65. http://dx.doi.org/10.5558/tfc2016-062.

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Coastal Pinus thunbergii (Japanese black pine) forests in the northern Shandong Peninsula of China recently experienced widespread natural regeneration failure. This study identifies critical factors that affect natural regeneration of P. thunbergii. Seeds from trees of various ages (13–32 years) were used to investigate the effects of age and burial depth in sand on germination and seedling establishment. Results show that seed density in 2–5 cm soil decreased with increased distance from the shoreline. Sand burial decreased seed germination but did not affect the relative growth rate of seed
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Zhang, Peng, Yuxiang Wen, Lei Wang, Hui Zhang, G. Geoff Wang, and Tonggui Wu. "Leaf Structural Carbohydrate Decreased for Pinus thunbergii along Coast–Inland Gradients." Forests 11, no. 4 (2020): 449. http://dx.doi.org/10.3390/f11040449.

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Although photosynthesis (carbohydrate production) decreases under wind load, it is unclear how carbohydrate categories allocation changes. We determined the leaf morphology (specific leaf area (SLA), needle thickness), anatomy (cuticle thickness, epidermal thickness), photosynthesis (effective quantum yield of Photosystem II (Y(II)), carbohydrate (structure carbohydrate (SC) and non-structure carbohydrate (NSC)), and environmental variables in Pinus thunbergii plantations from coast to inland, with wind speed decreasing. As expected, wind, accounting for 19–69% of the total variation, was the
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Suzuki, Kazuo, Daisuke Sakaue, Toshihiro Yamada, and Yu Wang. "Influence of fungi on multiplication and distribution of the pine wood nematode, Bursaphelenchus xylophilus, in axenized Pinus thunbergii cuttings." Nematology 7, no. 6 (2005): 809–17. http://dx.doi.org/10.1163/156854105776186343.

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AbstractInfluence of fungi on multiplication and distribution of the pine wood nematode (PWN), Bursaphelenchus xylophilus, was investigated in Pinus thunbergii cuttings. Axenized nematodes and/or one of two fungi isolated from healthy and PWN-killed P. thunbergii were inoculated together into autoclaved cuttings. A close relationship between the existence and distribution of fungal hyphae, and the multiplication and distribution of PWN was observed. The PWN did not multiply when only axenized nematodes were inoculated in the absence of fungi. When fungi were present, PWN population size increa
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Wen, Tong-Yue, Xin-Yu Wang, Xiao-Qin Wu, and Jian-Ren Ye. "Transcriptomic Profiling Reveals That the Differentially Expressed PtNAC9 Transcription Factor Stimulates the Salicylic Acid Pathway to Enhance the Defense Response against Bursaphelenchus xylophilus in Pines." Forests 15, no. 9 (2024): 1538. http://dx.doi.org/10.3390/f15091538.

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Pinus, a conifer, dominates the world’s forest ecosystems. But it is seriously infected with pine wood nematode (PWN). Transcription factors (TFs) are key regulators in regulating plant resistance. However, the molecular mechanism of TFs remains thus far unresolved in P. thunbergii inoculated with Bursaphelenchus xylophilus. Here, we used RNA-seq technology to identify differentially expressed TFs in resistant and susceptible pines. The results show that a total of 186 differentially expressed transcription factors (DETFs), including 99 upregulated and 87 downregulated genes were identified. G
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Yoon, Chan Jong, Won Seok Choi, Hyun Sik Kang, et al. "Pinus thunbergii Parl. Extracts Reduce Acute Inflammation by Targeting Oxidative Stress." Evidence-Based Complementary and Alternative Medicine 2021 (January 13, 2021): 1–10. http://dx.doi.org/10.1155/2021/7924645.

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Pinus thunbergii Parl. (PTP) has traditionally been used for edible and medicinal purposes to treat several disorders, including diabetes and neuralgia. Therefore, this study sought to evaluate the inhibitory effects of PTP leaf ethanol extracts on acute inflammation. Moreover, the reactive oxygen species (ROS) scavenging activity, superoxide dismutase (SOD) activity, lipopolysaccharide (LPS)-induced nitric oxide (NO) generation, and H2O2-induced lipid peroxidation capacity of PTP were assessed in vitro in RAW 264.7 macrophages. Our results suggest that PTP prevents cell damage caused by oxida
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Bak, Jing-Gi, Jin Kim, and Seung-Ho Ohk. "Preparation of Tuna Skin Byproduct Film Containing Pinus thunbergii Cone Extract." Biomedical Science Letters 26, no. 4 (2020): 360–67. http://dx.doi.org/10.15616/bsl.2020.26.4.360.

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Kawaguchi, Eriko, and Yu Ichihara. "Inhibition of Bursaphelenchus xylophilus Migration at the Nodes of Pinus thunbergii." Journal of the Japanese Forest Society 92, no. 1 (2010): 1–7. http://dx.doi.org/10.4005/jjfs.92.1.

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Yang, Haibo, Zhe Wang, Wei Song, Zhenli Zhao, and Yong Zhao. "Isolation of proanthocyanidins from Pinus thunbergii needles and tyrosinase inhibition activity." Process Biochemistry 100 (January 2021): 245–51. http://dx.doi.org/10.1016/j.procbio.2020.10.003.

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