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Journal articles on the topic 'Piroplas'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

KJEMTRUP, A. M., J. THOMFORD, T. ROBINSON, and P. A. CONRAD. "Phylogenetic relationships of human and wildlife piroplasm isolates in the western United States inferred from the 18S nuclear small subunit RNA gene." Parasitology 120, no. 5 (2000): 487–93. http://dx.doi.org/10.1017/s003118209900582x.

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The 18S nuclear small subunit ribosomal RNA gene of piroplasms from wildlife and human cases of babesiosis in the western USA were isolated by PCR and sequenced. Phylogenetic analyses of these sequences and comparisons with sequences from other Babesia and Theileria species revealed that piroplasm isolates from the human cases were indistinguishable from some of the isolates from the western wildlife species, most notably the isolates from mule deer (Odocoileus hemionus). These results suggest that large ungulates may serve as reservoirs for human piroplasm infection. The western piroplasm iso

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BIRKENHEUER, A. J., H. S. MARR, N. HLADIO, and A. E. ACTON. "Molecular evidence of prevalent dual piroplasma infections in North American raccoons (Procyon lotor)." Parasitology 135, no. 1 (2007): 33–37. http://dx.doi.org/10.1017/s0031182007003538.

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SUMMARYBased on 18S rRNA sequence analyses 2 distinct genotypes of piroplasms have been described in raccoons. One genotype resides in the Babesia sensu stricto clade and the other in the Babesia microti-like clade. Since these organisms appear morphologically indistinguishable, it is unclear which strain is responsible for the majority of the infections in raccoons. In order to overcome these limitations we performed a molecular survey of raccoons using polymerase chain reaction assays specific for each genotype. We tested blood samples from 41 wild raccoons trapped in eastern North Carolina

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Kaynar, Ö., T. Güldürw, and T. Karapinar. "Investigation of lectin activity in Theileria annulata piroplasms." Acta Veterinaria Hungarica 53, no. 1 (2005): 53–63. http://dx.doi.org/10.1556/avet.53.2005.1.6.

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Adhesion to target cells is an essential step in the pathogenesis of many protozoal infections. Some protozoa have been reported to have a lectin activity involved in their attachment to the cell surface. The ligand-receptor interaction involved in Theileria annulata infection is unclear at present, in spite of the fact that some aspects of the process have been investigated. To this end, T. annulata piroplasms have been screened for lectin activity. Blood taken from infected cattle was first depleted of leukocytes and then subjected to ammonium chloride lysis in order to isolate the piroplasm

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Palmer, João Pedro, Gilberto Gazêta, Marcos André, et al. "Piroplasm Infection in Domestic Cats in the Mountainous Region of Rio de Janeiro, Brazil." Pathogens 11, no. 8 (2022): 900. http://dx.doi.org/10.3390/pathogens11080900.

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Piroplasm infections in domestic cats have been reported with increasing frequency in numerous countries. However, in some states of Brazil, little information is available about the occurrence of these parasites. Blood samples were collected from 250 cats treated at a private clinic in the mountainous region of Rio de Janeiro. The samples were each subjected to a blood count, microscopic examination, and molecular research on piroplasms. The animals’ clinical histories and epidemiological information were analyzed to identify the risk factors associated with infection. Ticks were recovered du

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5

Garrett, Kayla B., Justin Brown, Mourad Gabriel, et al. "Diversity of Babesia spp. in skunks from selected states in the United States of America." Parasite 31 (2024): 42. http://dx.doi.org/10.1051/parasite/2024043.

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Babesia species are intraerythrocytic protozoan parasites that infect a variety of hosts. The goal of this study was to evaluate the piroplasm species present in skunks in various states in the United States and determine whether there was any geographic variation. Spleen, whole blood, or blood on filter paper were received from Pennsylvania, Kentucky, North Carolina, South Carolina, Georgia, Missouri, Louisiana, Texas, Kansas, and California, and were tested for Babesia sp. We tested four species of skunks including striped skunk (Mephitis mephitis, n = 72), eastern spotted skunk (Spilogale p

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BIRKENHEUER, A. J., C. A. HARMS, J. NEEL, et al. "The identification of a genetically unique piroplasma in North American river otters (Lontra canadensis)." Parasitology 134, no. 5 (2006): 631–35. http://dx.doi.org/10.1017/s0031182006002095.

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SUMMARYDuring a routine health check of a wild-caught North American river otter (Lontra canadensis) small piroplasms were noted within erythrocytes. Analyses of the 18S ribosomal ribonucleic acid (rRNA) gene sequences determined that this was a genetically unique organism most closely related to Babesia microti-like parasites found in other small carnivores. Subsequently 39 wild-trapped North American river otters from North Carolina were tested for the presence of piroplasma deoxyribonucleic acid (DNA) via polymerase chain reaction and piroplasma DNA was detected in 82% (32/39) of these samp

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Fernández, Nélida, Belen Revuelta, Irene Aguilar, et al. "Babesia and Theileria Identification in Adult Ixodid Ticks from Tapada Nature Reserve, Portugal." Pathogens 11, no. 2 (2022): 222. http://dx.doi.org/10.3390/pathogens11020222.

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This study, conducted in a nature reserve in southern Portugal, investigated the frequency and diversity of tick-borne piroplasms in six species of adult ixodid ticks removed from 71 fallow deer (Dama dama) and 12 red deer (Cervus elaphus), collected over the period 2012–2019. The majority of 520 ticks were Ixodes ricinus (78.5%), followed by Rhipicephalus sanguineus sensu lato, Hyalomma lusitanicum, Haemaphysalis punctata, Dermacentor marginatus, and Ixodes hexagonus. The R. sanguineus ticks collected from the deer were clearly exophilic, in contrast to the endophilic species usually associat

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Ghafar, Abdul, Anson V. Koehler, Ross S. Hall, Charles G. Gauci, Robin B. Gasser, and Abdul Jabbar. "Targeted Next-Generation Sequencing and Informatics as an Effective Tool to Establish the Composition of Bovine Piroplasm Populations in Endemic Regions." Microorganisms 9, no. 1 (2020): 21. http://dx.doi.org/10.3390/microorganisms9010021.

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Protists of the genera Babesia and Theileria (piroplasms) cause some of the most prevalent and debilitating diseases for bovines worldwide. In this study, we established and used a next-generation sequencing-informatic approach to explore the composition of Babesia and Theileria populations in cattle and water buffalo in a country (Pakistan) endemic for these pathogens. We collected individual blood samples from cattle (n = 212) and water buffalo (n = 154), extracted genomic DNAs, PCR-amplified the V4 hypervariable region of 18S small subunit rRNA gene from piroplasms, sequenced amplicons usin

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Lemos, Tatiana Didonet, Helena Keiko Toma, Renata Quintela Assad, Adrianna Vieira da Silva, Rafael Gomes Bartolomeu Corrêa, and Nádia Regina Pereira Almosny. "Clinical and hematological evaluation of Rangelia vitalii-naturally infected dogs in southeastern Brazil." Revista Brasileira de Parasitologia Veterinária 26, no. 3 (2017): 307–13. http://dx.doi.org/10.1590/s1984-29612017040.

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Abstract Rangelia vitalii, a tick-borne piroplasm that infects dogs, has been recently molecularly characterized in Brazil, Uruguay and Argentina. Studies on molecular characterization of these piroplasms in different Brazilian regions are scarce. The aim of this study was to evaluate clinical and hematological changes in dogs caused by R. vitalii in the mountainous region of the state of Rio de Janeiro. Blood samples from 36 dogs were evaluated for piroplasms and hematological disorders using light microscopy and molecular analysis. Blood samples from all the animals included in this study we

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Jenkins, Cheryl, Melinda L. Micallef, Matthew P. Padula, and Daniel R. Bogema. "Characterisation of the Theileria orientalis Piroplasm Proteome across Three Common Genotypes." Pathogens 11, no. 10 (2022): 1135. http://dx.doi.org/10.3390/pathogens11101135.

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Theileria orientalis is an emerging apicomplexan pathogen of cattle occurring in areas populated by the principal vector tick, Haemaphysalis longicornis. Unlike transforming Theileria spp. that induce cancer-like proliferation of lymphocytes via their schizont stage, T. orientalis destroys host erythrocytes during its piroplasm phase resulting in anaemia. The underlying pathogenic processes of T. orientalis infection are poorly understood; consequently, there are no vaccines for prevention of T. orientalis infection and chemotherapeutic options are limited. To identify antigens expressed durin

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Demeter, Zoltán, Elena Palade, Éva Balogh, et al. "Postmortem small babesia-like morphology of Babesia canis — Short communication." Acta Veterinaria Hungarica 59, no. 4 (2011): 427–32. http://dx.doi.org/10.1556/avet.2011.029.

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Here we report a case of canine babesiosis with unusual morphology of the causative agent. A male, seven-week-old Labrador retriever puppy, exhibiting severe anaemia and haemoglobinuria, was presented at the Clinic of Internal Medicine in February 2011. The puppy was euthanised. The most relevant pathological changes were icterus, severe splenomegaly, generalised lymphadenopathy and haemoglobin nephrosis. Samples were collected from various organs for histology within one hour post mortem. Impression smears were also prepared from the spleen after overnight storage at 4 °C. Tissue sections and

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Facile, Veronica, Martina Magliocca, Filippo Maria Dini, et al. "Molecular Diagnosis and Identification of Equine Piroplasms: Challenges and Insights from a Study in Northern Italy." Animals 15, no. 3 (2025): 437. https://doi.org/10.3390/ani15030437.

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Equine piroplasmosis is a tick-borne disease caused by Babesia and Theileria species. Despite its presence in Europe, no laboratory testing is required for animal movement, even though some countries remain free of this disease. Differentiating between species and genotypes is crucial to determine the most effective treatment, as dosage, active compounds, and duration vary. However, diagnosis is often challenging due to genetic variability and the limited sensitivity of molecular methods. The aims of this study were to compare the performances of different molecular diagnostic tests to identif

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Kudaybergenov, Timur Yaqip uli, and Sobirjon Ibadullaevich Mavlanov. "Prevalence and prevention measures of pyroplasmidosis in Karakalpakistan." JOURNAL OF UNIVERSAL SCIENCE RESEARCH 1, no. 10 (2023): 72–76. https://doi.org/10.5281/zenodo.8403598.

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Piroplasmidiosis, commonly referred to as "tick fever," is a parasitic disease that affects a wide range of animals, including livestock and domestic pets.These parasites are transmitted through the bite of infected ticks and can cause serious health problems in affected animals. In this article, we will explore the spread of piroplasmidiosis and the crucial preventive measures that can help mitigate its impact on animal populations. The huge role of leading scientists in the study of protozooses.the development of methods of therapy and prevention, as well as immunobiological pathog

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Famuyide, I. M., M. I. Takeet, A. O. Talabi, and E. B. Otesile. "Molecular Detection and Identification of Piroplasms in Semi-Intensively Managed Cattle from Abeokuta, Nigeria." Folia Veterinaria 64, no. 4 (2020): 1–8. http://dx.doi.org/10.2478/fv-2020-0031.

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AbstractPiroplasmosis is a tick-borne haemolytic disease caused by different species of the Babesia and Theileria genera. Data on the prevalence of bovine piroplasms and their genetic diversity are scanty in Nigeria. Hence, this study reported the detection of some piroplasms in the blood of cattle in Abeokuta, Nigeria by the polymerase chain reaction (PCR). Blood samples were collected from 252 cattle and subjected to DNA extraction followed by PCR amplification of the partial region of 18S rRNA of the haemoprotozoans. Selected positive amplicons were unidirectionally sequenced and compared t

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Wu, Xuanchen, Jun Xu, Lixin Su, et al. "First Molecular Evidence of Babesia caballi and Theileria equi in Imported Donkeys from Kyrgyzstan." Pathogens 13, no. 9 (2024): 713. http://dx.doi.org/10.3390/pathogens13090713.

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Equine piroplasmosis (EP) is an important tick-borne disease of equids, caused by Theileria equi, Theileria haneyi, and Babesia caballi. Nonetheless, there has been a scarcity of systematic reports on EP parasites in donkeys in Kyrgyzstan, Central Asia. In this study, piroplasms were screened in 1900 blood samples from imported donkeys from the Osh Oblast (southwestern Kyrgyzstan) by targeting partial 18S ribosomal RNA using the polymerase chain reaction (PCR). Through molecular and phylogenetic analyses, all positive samples were sequenced to identify the species and genotypes. The results in

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16

Kubota, S., C. Sugimoto, and M. Onuma. "Population dynamics ofTheileria sergentiin persistently infected cattle and vector ticks analysed by a polymerase chain reaction." Parasitology 112, no. 5 (1996): 437–42. http://dx.doi.org/10.1017/s0031182000076897.

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SUMMARYTheileria sergentiShintoku stock consists of 2 parasite populations bearing 2 atlelic forms of p33/32, an immunodominant piroplasm surface protein. Parasite population changes during parasite passages among cattle and tick vectors, and during persistent infection in individual calves were analysed by using allele-specific polymerase chain reaction (PCR). The parasite DNAs were prepared from piroplasms from calves which had been infected with Shintoku stock by inoculation of sporozoite stabilates or parasitized erythrocytes, and from sporozoite stabilates which had been prepared from Shi

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17

Stevanovic, Oliver, and Andrea Radalj. "Molecular evidence of Theileria orientalis infection in cattle from Bosnia and Herzegovina." Veterinarski glasnik, no. 00 (2023): 1. http://dx.doi.org/10.2298/vetgl220708001s.

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There are no data on the distribution of oriental theileriosis in cattle from Bosnia and Herzegovina. For the first time, a possible endemic focus of Theileria orientalis infection was confirmed in specific areas of Pale municipality, Sarajevo-Romanija region, Bosnia and Herzegovina. Selective sampling included 30 cattle from 10 smallholder farms in several locations in Pale municipality. The total of 30 whole blood samples were screened for the presence of piroplasms using commercial PCR. Positive PCR products were sequenced in both directions, the sequences were analyzed and a phylogenetic t

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18

Fernandes, Mayara Marques Pereira, Marcelo Renan Serrate Rodrigues, Jessica Damiana Marinho Valente, et al. "Babesia vogeli in dogs from Rio Branco, South-west Amazonia, Brazil." Semina: Ciências Agrárias 42, no. 6 (2021): 3527–34. http://dx.doi.org/10.5433/1679-0359.2021v42n6p3527.

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This is the first report of Babesia vogeli molecular detection in dogs from the state of Acre, northern Brazil. This study aimed to perform the molecular detection of Babesia vogeli in dogs in the municipality of Rio Branco, Acre. Blood samples were collected from 47 dogs presenting with clinical signs comparable to hemoparasitosis. These were dogs which were attended in veterinary clinics from Rio Branco municipality, Acre. Physical examinations, packed cell volume (PCV) determination, platelet number estimation, hemoparasite investigation in the blood (collected from the pinna and peripheral

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19

Spada, Eva, Daniela Proverbio, Paola Galluzzo, et al. "Frequency of PiroplasmsBabesia microtiandCytauxzoon felisin Stray Cats from Northern Italy." BioMed Research International 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/943754.

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Emerging diseases caused by piroplasms pose a health risk for man and other animals, and domestic cats have been proposed as potential reservoirs for some piroplasm infections. The aim of this study was to identify the frequency of the piroplasmsBabesia microtiandCytauxzoon felisin stray cats from northern Italy and to identify possible risk factors associated with these infections. Blood samples from 260 stray cats enrolled in a trap-neuter-release (TNR) program in northern Italy were examined with conventional PCR for the presence ofBabesia microtiandCytauxzoon felisDNA. No sample (0.0%) tes

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Khan, R. A., W. Threlfall, and W. S. Whitty. "Hematozoa of deep-sea demersal fish of the Northwest Atlantic Ocean." Canadian Journal of Zoology 70, no. 10 (1992): 1926–33. http://dx.doi.org/10.1139/z92-262.

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A total of 410 deep-sea demersal fish comprising 54 species was taken at depths of 1000–3200 m from two locations in the Northwest Atlantic Ocean and examined for hematozoans. Prevalences of 29% of the total fish and 61% of the species examined were observed. Piroplasms (Haemohormidium spp.) were the most common parasites (26% infection), with hemogregarine, trypanosome, and cryptobia infections being considerably lower. Haemogregarina marshalllairdi sp.nov. is described from Nezumia bairdi and Macrourus berglax. An enigmatic intraerythrocytic parasite was observed in one fish. A comparison of

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Abdel-Shafy, Sobhy, Hend H. A. M. Abdullah, Mohamed K. Elbayoumy, et al. "Molecular Epidemiological Investigation of Piroplasma spp. and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks." Pathogens 11, no. 10 (2022): 1194. http://dx.doi.org/10.3390/pathogens11101194.

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Piroplasmosis and anaplasmosis are serious tick-borne diseases (TBDs) that are concerning for the public and animal health. This study aimed to detect the molecular prevalence and epidemiological risk factors of Piroplasma and Anaplasma species in animal hosts and their associated ticks in Egypt. A total of 234 blood samples and 95 adult ticks were collected from animal hosts (112 cattle, 38 sheep, 28 goats, 26 buffaloes, 22 donkeys, and 8 horses) from six provinces of Egypt (AL-Faiyum, AL-Giza, Beni-Suef, Al-Minufia, Al-Beheira, and Matruh). Blood and tick samples were investigated by polymer

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O'Donoghue, Peter. "Haemoprotozoa: Making biological sense of molecular phylogenies." International Journal for Parasitology: Parasites and Wildlife 6, no. 3 (2017): 241–56. https://doi.org/10.5281/zenodo.13535368.

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(Uploaded by Plazi for the Bat Literature Project) A range of protistan parasites occur in the blood of vertebrates and are transmitted by haematophagous invertebrate vectors. Some 48 genera are recognized in bood primarily on the basis of parasite morphology and host specificity; including extracellular kinetoplastids (trypanosomatids) and intracellular apicomplexa (haemogregarines, haemococcidia, haemosporidia and piroplasms). Gene sequences are available for a growing number of species and molecular phylogenies often link parasite and host or vector evolution. This review endeavours to reco

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O'Donoghue, Peter. "Haemoprotozoa: Making biological sense of molecular phylogenies." International Journal for Parasitology: Parasites and Wildlife 6, no. 3 (2017): 241–56. https://doi.org/10.5281/zenodo.13535368.

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(Uploaded by Plazi for the Bat Literature Project) A range of protistan parasites occur in the blood of vertebrates and are transmitted by haematophagous invertebrate vectors. Some 48 genera are recognized in bood primarily on the basis of parasite morphology and host specificity; including extracellular kinetoplastids (trypanosomatids) and intracellular apicomplexa (haemogregarines, haemococcidia, haemosporidia and piroplasms). Gene sequences are available for a growing number of species and molecular phylogenies often link parasite and host or vector evolution. This review endeavours to reco

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Jasik, Krzysztof P., Anna Kleczka, and Aleksandra Franielczyk. "Histopathological Aspects of the Influence of Babesia microti on the Placentas of Infected Female Rats." Veterinary Sciences 11, no. 1 (2024): 18. http://dx.doi.org/10.3390/vetsci11010018.

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Babesiosis is perceived mainly an animal disease; however, awareness that Babesia spp. parasites that can cause diseases in humans is increasing significantly. Babesiosis is spread by the bite of an infected tick (Ixodes spp.), but it can also be transmitted by transfusion of infected blood and from an infected mother to her child during pregnancy or childbirth. The parasites multiply in the bloodstream and destroy red blood cells. This study aimed to assess the influence of Babesia microti on the histological structure of the placenta. Histopathological material collected from pregnant rats i

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Hornok, Sándor, Alexandra Corduneanu, Jenő Kontschán, et al. "Analyses of separate and concatenated cox1 and 18S rRNA gene sequences indicate that the bat piroplasm Babesia vesperuginis is phylogenetically close to Cytauxzoon felis and the ‘prototheilerid’ Babesia conradae." Acta Veterinaria Hungarica 66, no. 1 (2018): 107–15. http://dx.doi.org/10.1556/004.2018.010.

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Babesia vesperuginis is the only piroplasm known to infect bats. Unlike most members of the genus Babesia, it is probably transmitted by a soft tick species (i.e. Argas vespertilionis). Recently, two studies have been conducted to clarify the phylogenetic status of this species, and both agreed on placing it into a basal position among Babesia sensu stricto (s.s.). However, several important groups of piroplasms were not included in the already reported phylogenetic trees of B. vesperuginis isolates. Therefore, the aim of the present study was to amplify an approx. 950-bp fragment of the cytoc

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Seo, Min-Goo, Oh-Deog Kwon, and Dongmi Kwak. "Genotypic Analysis of Piroplasms and Associated Pathogens from Ticks Infesting Cattle in Korea." Microorganisms 8, no. 5 (2020): 728. http://dx.doi.org/10.3390/microorganisms8050728.

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Tick-borne pathogens (TBPs) impose an important limitation to livestock production worldwide, especially in subtropical and tropical areas. Earlier studies in Korea have examined TBPs residing in ticks and animals; however, information on multiple TBPs in ticks infesting cattle is lacking. This study assessed the prevalence of TBPs in ticks parasitizing cattle. A total of 576 ticks, including 340 adults and 236 nymphs, were collected from cattle in Korea between 2014 and 2018. All ticks collected were identified as Haemaphysalis longicornis based on their morphological and molecular characteri

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S., Vaisakhi Vikraman,, Deepa, P.M., Rathish, R.L., Janus, A., and Rajasekhar, R. "Haematological and Biochemical Profiles, Including Vitamin A and Zinc Status, in Clinical and Subclinical Oriental Theileriosis in Cattle." Journal of Advances in Biology & Biotechnology 27, no. 11 (2024): 919–25. http://dx.doi.org/10.9734/jabb/2024/v27i111675.

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Aim: The present study investigated the clinical, haematological and biochemical profiles in cattle infected with Theileria orientalis, correlating disease severity with various parameters. Study Design: A total of 32 clinically ill, 10 subclinically infected, and 10 healthy control animals were included. Place and Duration of Study: Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad, Kerala, between November 2023 to September 2024. Methodology: Theileria piroplasms, observ

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Adjou Moumouni, Paul Franck, Germaine Lim-Bamba Minoungou, Christian Enonkpon Dovonou, et al. "A Survey of Tick Infestation and Tick-Borne Piroplasm Infection of Cattle in Oudalan and Séno Provinces, Northern Burkina Faso." Pathogens 11, no. 1 (2021): 31. http://dx.doi.org/10.3390/pathogens11010031.

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In this study, cattle farms located in Oudalan and Séno, two provinces in the Sahel region, northern Burkina Faso, were surveyed. Cattle owners were interviewed, cattle were examined for tick infestation, and ticks as well as blood samples were collected during the dry season (October). Blood DNA samples were tested for Babesia and Theileria infections using nested PCRs and sequencing. A total of 22 herds, 174 Zebu cattle were investigated at 6 different sites. Overall, 76 cattle (43.7 %) from 18 farms (81.8%) were found infested with ticks. Cattle in Séno, adult cattle (>5 years) and those

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Martins, Tiago M., Virgílio E. do Rosário, and Ana Domingos. "Identification of papain-like cysteine proteases from the bovine piroplasm Babesia bigemina and evolutionary relationship of piroplasms C1 family of cysteine proteases." Experimental Parasitology 127, no. 1 (2011): 184–94. http://dx.doi.org/10.1016/j.exppara.2010.07.012.

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Idoko, Idoko S., Richard E. Edeh, Andrew M. Adamu, et al. "Molecular and Serological Detection of Piroplasms in Horses from Nigeria." Pathogens 10, no. 5 (2021): 508. http://dx.doi.org/10.3390/pathogens10050508.

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Equine piroplasmosis, an economically important disease of equids caused by the hemoprotozoan parasites Theileria equi, T. haneyi, and Babesia caballi, has a worldwide distribution. These parasites are transmitted by ixodid ticks. To improve the detection of horses in Nigeria exposed to piroplasm parasites, 72 horses with variable clinical signs of piroplasmosis were sampled from Northwest and Northcentral Nigeria and tested by nPCR and cELISA. Blood and serum samples were collected from each horse via jugular venesection. Individually, nPCR or cELISA failed to identify all horses exposed to p

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Hornok, S., Renate Edelhofer, G. Földvári, Anja Joachim, and R. Farkas. "Serological evidence for Babesia canis infection of horses and an endemic focus of B. caballi in Hungary." Acta Veterinaria Hungarica 55, no. 4 (2007): 491–500. http://dx.doi.org/10.1556/avet.55.2007.4.8.

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In order to evaluate the seroconversion of horses to Babesia caballi and B. canis in Hungary, blood samples were collected from 371 animals on 23 different locations of the country. The presence of antibodies to B. caballi was screened with a competitive ELISA. All 29 positive samples came from one region (the Hortobágy). The prevalence of infection did not show correlation with sexes, and reached 100% in the age group of 2–5 years. Babesia canis -specific antibodies were demonstrated by IFAT in 6.74% of animals kept in 7 regions. The titres were low or medium level (1:40 to 1:160), indicating

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Fidalgo Garra, Estela, and Elisa Fernández Rei. ""Estás de bo ano": una aproximación al piropo en gallego desde la (des)cortesía." Círculo de Lingüística Aplicada a la Comunicación 99 (September 17, 2024): 109–24. http://dx.doi.org/10.5209/clac.89210.

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El propósito (des) cortés de los piropos suscita debate, ya que, a pesar de ser una valoración positiva de la persona destinataria, se entienden como un acto de habla arriesgado que puede poner en peligro la imagen tanto de la persona enunciadora como de la destinataria; además de ser considerados por muchas personas una práctica masculina abusiva hacia las mujeres que contribuye a la perpetuación de la desigualdad de género. Este estudio se acerca a la práctica discursiva de los piropos en gallego entre gente joven y analiza en qué contextos las/los hablantes consideran adecuado o no realizar

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Hornok, Sándor, Attila D. Sándor, Gábor FÖldvári, et al. "First broad-range molecular screening of tick-borne pathogens in Ixodes (Pholeoixodes) kaiseri, with special emphasis on piroplasms." Acta Veterinaria Hungarica 68, no. 1 (2020): 30–33. http://dx.doi.org/10.1556/004.2020.00003.

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AbstractRecently, the occurrence of Ixodes (Pholeoixodes) kaiseri has been reported for the first time in several European countries, but data on the molecular analysis of this hard tick species are still lacking. Therefore, in this study DNA extracts of 28 I. kaiseri (collected from dogs and red foxes in Germany, Hungary and Romania) were screened with reverse line blot hybridisation (RLB), PCR and sequencing for the presence of 43 tick-borne pathogens or other members of their families from the categories of Anaplasmataceae, piroplasms, rickettsiae and borreliae. Rickettsia helvetica DNA was

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Fadiji, Oyinkansola, Ayodele S. Babalola, Foluke A. Akande, Sunday O. Olaniyan, and Sammy O. Sam-Wobo. "Molecular Epidemiology of Babesia and Theileria spp among Slaughtered Cattle in Abeokuta, Southwestern Nigeria." Sahel Journal of Life Sciences FUDMA 3, no. 1 (2025): 563–71. https://doi.org/10.33003/sajols-2025-0301-68.

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Cattle are vulnerable to tick-borne pathogens like Babesia and Theileria, which pose health threats and zoonotic risks. This study examined piroplasm infections, tick infestations, and demographics among cattle in Abeokuta, Ogun State, Nigeria. From October 2018 to February 2021, 300 slaughtered cattle were analyzed. Blood samples were assessed by microscopy and PCR, while tick identification relied on morphology. Demographic data, including breed, sex, and body condition, along with tick presence and location, were recorded. Findings showed 73.8% were White Fulani cattle. Tick infestation aff

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Mayock, Ellen. "Piropos académicos." Letras Femeninas 39, no. 2 (2013): 245. http://dx.doi.org/10.2307/44733664.

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Beck, Ana, Doroteja Huber, Adam Polkinghorne, et al. "The prevalence and impact of Babesia canis and Theileria sp. in free-ranging grey wolf (Canis lupus) populations in Croatia." Parasites & Vectors 10, no. 1 (2017): 168. https://doi.org/10.1186/s13071-017-2106-8.

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<strong>Background: </strong> <i>Babesia</i> spp. and <i>Theileria</i> spp. are important emerging causes of disease in dogs. Alongside these domesticated hosts, there is increasing recognition that these piroplasms can also be found in a range of wild animals with isolated reports describing the presence of these pathogen in foxes (<i>Vulpes vulpes</i>) and captive grey wolves (<i>Canis lupus</i>). The prevalence and impact of these infections in free-ranging populations of canids are unknown. To gain a better insight into the epidemiology and pathogenesis of piroplasm infections in free-rang

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Abate, Hugo Luca, Nelson Jessé Rodrigues dos Santos, Danilo Rodrigues Barros Brito, et al. "Theileria sp. in water buffaloes from Maranhão State, northeastern Brazil." Revista Brasileira de Parasitologia Veterinária 27, no. 4 (2018): 593–96. http://dx.doi.org/10.1590/s1984-296120180075.

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Abstract Anaplasma marginale and piroplasm species are widespread among Brazilian cattle herds. Both of these tick-borne pathogens hamper livestock production and cause a significant economic impact. Although buffaloes have demonstrated a high level of adaptability, data on tick-borne pathogens are scarcely reported in Brazil. Thus, the aim of this study was to screen water buffaloes from the state of Maranhão for piroplasm and A. marginale occurrence using PCR assays. All samples were negative for A. marginale. One of the 287 (0.35%) water buffaloes tested was positive for Theileria sp. Seque

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Rizk, Mohamed Abdo, Shimaa Abd El-Salam El-Sayed, and Ikuo Igarashi. "Effects of Methanolic Extract from Turmeric (Curcuma longa) against the In Vitro Multiplication of Several Babesia Species and Theileria equi." Parasitologia 1, no. 4 (2021): 188–96. http://dx.doi.org/10.3390/parasitologia1040020.

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Anti-piroplasm drugs currently on the market have proven toxicity to the host and parasite resistance. Plants are possible sources of novel drugs. Subsequently, a novel strategy should be used to find new anti-piroplasm agents that are both effective and safe. In the present study, we have evaluated the effect of turmeric (Curcuma longa) methanolic extract on the in vitro growth of Babesia (B.) bovis, B. divergens, B. caballi, and Theileria (T.) equi. The in vitro inhibitory effectiveness of turmeric was assessed using a fluorescence test. The enhancement in the in vitro inhibitory efficacy of

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Omar Abdallah, Mirza, Qingli Niu, Peifa Yu, et al. "Identification of piroplasm infection in questing ticks by RLB: a broad range extension of tick-borne piroplasm in China?" Parasitology Research 115, no. 5 (2016): 2035–44. http://dx.doi.org/10.1007/s00436-016-4947-z.

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RJEIBI, MOHAMED R., SAFA AMAIRIA, MARIEM ROUATBI, FATMA BEN SALEM, MOEZ MABROUK, and MOHAMED GHARBI. "Molecular prevalence and genetic characterization of piroplasms in dogs from Tunisia." Parasitology 143, no. 12 (2016): 1622–28. http://dx.doi.org/10.1017/s003118201600113x.

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SUMMARYIn this study, the prevalence of piroplasms in dogs was assessed using polymerase chain reaction (PCR) to identifyBabesiaandTheileriaspecies in 200 dogs from Northern and Central Tunisia between spring and autumn 2014. The overall molecular prevalence for piroplasms was 14·5% ± 0·05 (29/200); PCR detected 2 species, namelyBabesia vogeliandTheileria annulatawith an overall prevalence of 12·5 ± 0·04 and 2% ± 0·02, respectively. No differences in the molecular prevalences ofB. vogeliwere revealed for age and sex (P> 0·05). The molecular prevalence ofB. vogeliwas significantly higher in

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Criado-Fornelio, A. "The “expanding universe” of piroplasms." Veterinary Parasitology 119, no. 4 (2004): 337–45. http://dx.doi.org/10.1016/j.vetpar.2003.11.015.

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Paparini, Andrea, Linda M. McInnes, Daniela Di Placido, et al. "Piroplasms of New Zealand seabirds." Parasitology Research 113, no. 12 (2014): 4407–14. http://dx.doi.org/10.1007/s00436-014-4118-z.

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Papadopoulos, Byron, Michel Brossard, and Nan Marie Perié. "Piroplasms of domestic animals in the Macedonia region of Greece 2. Piroplasms of cattle." Veterinary Parasitology 63, no. 1-2 (1996): 57–66. http://dx.doi.org/10.1016/0304-4017(95)00845-4.

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Oliveira, A. R., G. R. G. Pinheiro, T. D. Souza, M. C. Flecher, and R. L. Santos. "Abortion in association with transplacental Theileria equi infection in a mare from the State of Espírito Santo, southeast Brazil: case report." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 71, no. 2 (2019): 369–73. http://dx.doi.org/10.1590/1678-4162-10696.

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ABSTRACT Equine piroplasmosis is a tick-borne disease caused by the protozoan parasites Babesia caballi and Theileria equi. We report a case of abortion in association with transplacental Theileria equi infection in a mare from the State of Espírito Santo, southeast Brazil. An apparently healthy mare aborted at the tenth month of gestation. At necropsy, the subcutaneous tissue, skeletal muscles, and visible mucosae of the aborted fetus were pale, and there was moderate hydrothorax and marked splenomegaly. Microscopic findings included splenic lymphoid hyperplasia and nephrosis. Merozoite-infec

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Níkleva, Dimitrinka, and Francisco J. Rodríguez-Muñoz. "Las secuencias piropo-respuesta y cumplido-respuesta: tipología y ejemplos." Moderna Språk 111, no. 2 (2017): 88–109. http://dx.doi.org/10.58221/mosp.v111i2.7744.

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El presente estudio pretende esbozar algunas diferencias culturales en los estilos adulatorios y en la manera de formular, recibir y rechazar piropos y cumplidos en distintas lenguas y culturas. Se presenta una clasificación propia de las características de los piropos y se establece una tipología de las secuencias piropo-respuesta y cumplido-respuesta, acompañada con ejemplos en distintas lenguas: español, inglés, búlgaro y polaco. A pesar de que el estudio se centra en estos cuatro idiomas, se hacen alusiones a más lenguas con sus respectivas diferencias culturales y estrategias de las respu

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Khamassi Khbou, Médiha, Mariem Rouatbi, Rihab Romdhane, et al. "Tick Infestation and Piroplasm Infection in Barbarine and Queue Fine de l’Ouest Autochthonous Sheep Breeds in Tunisia, North Africa." Animals 11, no. 3 (2021): 839. http://dx.doi.org/10.3390/ani11030839.

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As ticks and tick-borne pathogens affect the productivity of livestock, searching for genetically resistant breeds to infestation by ticks may represent an alternative to the overuse of chemical drugs. The aim of this study was to assess if there is a difference in tick infestation among the main sheep breeds in Tunisia. The study was carried out between April 2018 and January 2020 in 17 small to middle-sized sheep flocks from 3 regions across Tunisia. Four hundred and thirty-nine ear-tagged ewes from Barbarine (n = 288, 65.6%) and Queue Fine de l’Ouest (QFO) (n = 151, 34.4%) breeds were exami

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Papadopoulos, Byron, Michel Brossard, and Nan Marie Perié. "Piroplasms of domestic animals in the Macedonia region of Greece 3. Piroplasms of small ruminants." Veterinary Parasitology 63, no. 1-2 (1996): 67–74. http://dx.doi.org/10.1016/0304-4017(95)00846-2.

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Grynyshyn, Oleg, Michael Bratychak, Volodymyr Krynytskiy, and Volodymyr Donchak. "Petroleum resins for bitumens modification." Chemistry & Chemical Technology 2, no. 1 (2008): 47–53. http://dx.doi.org/10.23939/chcht02.01.047.

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The process of tar and industrial petroleum resin “Piroplast-2” joint oxidation has been investigated. The experimental results of the main regularities researches of the obtaining process of petroleum resins with carboxyl groups based on the pyrocondensate C9 fraction of hydrocarbon feedstock pyrolysis have been shown. It has been established that synthesized petroleum resins may be used for the modification of petroleum bitumens.

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Mongruel, Anna Cláudia Baumel, Priscila Ikeda, Keyla Carstens Marques de Sousa, et al. "Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil." Revista Brasileira de Parasitologia Veterinária 27, no. 4 (2018): 505–13. http://dx.doi.org/10.1590/s1984-296120180069.

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Abstract Arthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic a

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SKILTON, R. A., A. J. MUSOKE, C. W. WELLS, et al. "A 32 kDa surface antigen of Theileria parva: characterization and immunization studies." Parasitology 120, no. 6 (2000): 553–64. http://dx.doi.org/10.1017/s0031182099005934.

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Previous studies using monoclonal antibody (mAb) 4C9 specific for a 32 kDa antigen (p32) of Theileria parva demonstrated expression of the antigen on the surface of the sporozoite, making it a potential antigen for sporozoite neutralization. A full-length cDNA encoding the major merozoite/piroplasm surface antigen (mMPSA) of T. parva was cloned and expressed in bacteria. The expressed product reacted strongly with mAb 4C9, demonstrating identity between the p32 and mMPSA of T. parva. Using immunoblot analysis and immunoelectron microscopy with mAb 4C9 it was shown that the mMPSA is a major ant

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