Relevant bibliographies by topics / PKLAC1

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  1. Journal articles
  2. Dissertations / Theses

Academic literature on the topic 'PKLAC1'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 February 2022

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Journal articles on the topic "PKLAC1"

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Colussi, Paul A., and Christopher H. Taron. "Kluyveromyces lactis LAC4 Promoter Variants That Lack Function in Bacteria but Retain Full Function in K. lactis." Applied and Environmental Microbiology 71, no. 11 (2005): 7092–98. http://dx.doi.org/10.1128/aem.71.11.7092-7098.2005.

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ABSTRACT The strong LAC4 promoter (PLAC4) from Kluyveromyces lactis has been extensively used to drive expression of heterologous proteins in this industrially important yeast. A drawback of this expression method is the serendipitous ability of PLAC4 to promote gene expression in Escherichia coli. This can interfere with the process of assembling expression constructs in E. coli cells prior to their introduction into yeast cells, especially if the cloned gene encodes a protein that is detrimental to bacteria. In this study, we created a series of PLAC4 variants by targeted mutagenesis of thre
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Hidayah, Ulfa Lu’luatul, Nur Rohman, and Anita Dewi Utami. "PELEVELAN PEMAHAMAN KONSEP KOMPOSISI FUNGSI BERDASAR TAKSONOMI SOLO (STRUCTURE OF OBSERVES LEARNING OUTCOMES)." Journal of Mathematics Education and Science 3, no. 2 (2020): 95–102. http://dx.doi.org/10.32665/james.v3i2.162.

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This qualitative descriptive study aims to describe the level of student understanding of function composition based on the SOLO taxonomy. This research was conducted at Madrasah Aliyah Islamiyah Balen on science class X students school year 2019/2020 with 20 subjects. Sampling using stratified sampling techniques (conditional sample) and purposive sampling (sample aims) see the results of written tests that refer to the grid of test questions and sampling data sources with specific considerations. Analysis of the level of understanding with PKLM1, PKLM2, PKLR1, PKLR2, PKLE1, and PKLE2. Data a
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Read, Jeremiah D., Paul A. Colussi, Mehul B. Ganatra, and Christopher H. Taron. "Acetamide Selection of Kluyveromyces lactis Cells Transformed with an Integrative Vector Leads to High-Frequency Formation of Multicopy Strains." Applied and Environmental Microbiology 73, no. 16 (2007): 5088–96. http://dx.doi.org/10.1128/aem.02253-06.

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ABSTRACT The yeast Kluyveromyces lactis has been extensively used as a host for heterologous protein expression. A necessary step in the construction of a stable expression strain is the introduction of an integrative expression vector into K. lactis cells, followed by selection of transformed strains using either medium containing antibiotic (e.g., G418) or nitrogen-free medium containing acetamide. In this study, we show that selection using acetamide yields K. lactis transformant populations nearly completely comprised of strains bearing multiple tandem insertions of the expression vector p
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Kulik, Alena V., Olga B. Rus, and Anatoliy N. Evtushenkov. "Cloning of cDNA glucoamylase Aspergillus awamori into yeast integrative expression vector." Journal of the Belarusian State University. Biology, no. 3 (October 31, 2019): 59–66. http://dx.doi.org/10.33581/2521-1722-2019-3-59-66.

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We constructed pKLAC2-based integrative expression plasmid pKGLA-1 with glaA gene from Aspergillus awamori 466. The PCR amplification of the target gene glaA and restriction analysis proved pKGLA-1 construction. Linearised plasmid was used for the integrative transformation of chemically competent Kluyveromyces lactis GG799 cells. Colonies of cells transformed with pKGLA-1 plasmid were selected by growth on agar plates containing 5 mmol/L acetamide. Expression of the heterologous gene in K. lactis cells was visually assessed using medium containing 2 % starch. K. lactis cells containing integr
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Brabcová, Kateřina Pachnerová, Zuzana Jamborová, Anna Michaelidesová, et al. "RADIATION-INDUCED PLASMID DNA DAMAGE: EFFECT OF CONCENTRATION AND LENGTH." Radiation Protection Dosimetry 186, no. 2-3 (2019): 168–71. http://dx.doi.org/10.1093/rpd/ncz196.

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Abstract Plasmid DNA is commonly used as a simpler substitute for a cell in studies of early effects of ionizing radiation because it allows to determine yields of primary DNA lesions. Experimental studies often employ plasmids of different lengths, in different concentrations in the aqueous solution. Influence of these parameters on the heavy-ion induced yields of primary DNA damage has been studied, using plasmids pUC19 (2686 bp), pBR322 (4361 bp) and pKLAC2 (9107 bp) in 10 and 50 ng/μl concentration. Results demonstrate the impact of plasmid length, while no significant difference was obser
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Pidoux, A. L., M. LeDizet, and W. Z. Cande. "Fission yeast pkl1 is a kinesin-related protein involved in mitotic spindle function." Molecular Biology of the Cell 7, no. 10 (1996): 1639–55. http://dx.doi.org/10.1091/mbc.7.10.1639.

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We have used anti-peptide antibodies raised against highly conserved regions of the kinesin motor domain to identify kinesin-related proteins in the fission yeast Schizosaccharomyces pombe. Here we report the identification of a new kinesin-related protein, which we have named pkl1. Sequence homology and domain organization place pkl1 in the Kar3/ncd subfamily of kinesin-related proteins. Bacterially expressed pkl1 fusion proteins display microtubule-stimulated ATPase activity, nucleotide-sensitive binding, and bundling of microtubules. Immunofluorescence studies with affinity-purified antibod
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Almha, Restie, Wawan Setiawan Abdillah, and Khaerul Umam. "Iklim Komunikasi Berbasis Kearifan Lokal Studi Kasus Di Pusat Pelatihan Dan Pengembangan Dan Pemetaan Kompetensi Aparatur Sipil Negara LAN-RI Jatinangor." Ministrate: Jurnal Birokrasi dan Pemerintahan Daerah 3, no. 1 (2021): 34–41. http://dx.doi.org/10.15575/jbpd.v3i1.9621.

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This journal discusses the climate of communication of local wisdom-based organizations in Puslatbang PKASN LAN-RI Jatinangor. Especially the application of the value of local wisdom in the organizational communication climate at the PKATN LAN-RI Jatinangor Puslatbang. The purpose of this research is to find out how Climate Communication of Local Wisdom-based Organizations in PKlatN LAN-RI Jatinangor, to find out the barriers that occur in the climate of communication of local wisdom-based organizations in PKlatN LAN-RI Jatinangor, and find out the efforts made to overcome obstacles in climate
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Yukawa, Masashi, Chiho Ikebe, and Takashi Toda. "The Msd1–Wdr8–Pkl1 complex anchors microtubule minus ends to fission yeast spindle pole bodies." Journal of Cell Biology 209, no. 4 (2015): 549–62. http://dx.doi.org/10.1083/jcb.201412111.

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The minus ends of spindle microtubules are anchored to a microtubule-organizing center. The conserved Msd1/SSX2IP proteins are localized to the spindle pole body (SPB) and the centrosome in fission yeast and humans, respectively, and play a critical role in microtubule anchoring. In this paper, we show that fission yeast Msd1 forms a ternary complex with another conserved protein, Wdr8, and the minus end–directed Pkl1/kinesin-14. Individual deletion mutants displayed the identical spindle-protrusion phenotypes. Msd1 and Wdr8 were delivered by Pkl1 to mitotic SPBs, where Pkl1 was tethered throu
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Shirasugi, Yutaka, and Masamitsu Sato. "Kinetochore-mediated outward force promotes spindle pole separation in fission yeast." Molecular Biology of the Cell 30, no. 22 (2019): 2802–13. http://dx.doi.org/10.1091/mbc.e19-07-0366.

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Bipolar spindles are organized by motor proteins that generate microtubule-­dependent forces to separate the two spindle poles. The fission yeast Cut7 (kinesin-5) is a plus-end-directed motor that generates the outward force to separate the two spindle poles, whereas the minus-end-directed motor Pkl1 (kinesin-14) generates the inward force. Balanced forces by these antagonizing kinesins are essential for bipolar spindle organization in mitosis. Here, we demonstrate that chromosomes generate another outward force that contributes to the bipolar spindle assembly. First, it was noted that the cut
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10

Katayama, S., Y. Sugiyama, N. Hatano, T. Terachi, N. Sueyoshi, and I. Kameshita. "PKL01, an Ndr kinase homologue in plant, shows tyrosine kinase activity." Journal of Biochemistry 152, no. 4 (2012): 347–53. http://dx.doi.org/10.1093/jb/mvs075.

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Dissertations / Theses on the topic "PKLAC1"

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Rosa, Júlio César Câmara. "Obtenção de linhagens de Kluyveromyces lactis recombinantes produtoras de estreptavidina." Universidade Federal de Viçosa, 2007. http://locus.ufv.br/handle/123456789/5381.

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Made available in DSpace on 2015-03-26T13:52:02Z (GMT). No. of bitstreams: 1 texto completo.pdf: 609136 bytes, checksum: bfac4beb809c8b3d58b49895dc0cc2d1 (MD5) Previous issue date: 2007-03-09<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico<br>The high affinity interaction streptavidin-biotin provides an excellent system for industrial enzyme separation or immobilization involving a fused streptavidin-enzyme and a biotinylated support. In order to produce proteins with affinity to biotin, we have modified the commercial K. lactis system, pKLAC1 (New England Biolabs), a LAC4 p
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Colombo, Lívia Tavares. "Construção de linhagens de Kluyveromyces lactis Δku80 hospedeiras para produção de proteínas recombinantes: Análise da expressão da fusão estreptavidina-pectina liase." Universidade Federal de Viçosa, 2011. http://locus.ufv.br/handle/123456789/5335.

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Made available in DSpace on 2015-03-26T13:51:52Z (GMT). No. of bitstreams: 1 texto completo.pdf: 800491 bytes, checksum: 274cc18672a32ed45926e416e0de5305 (MD5) Previous issue date: 2011-02-15<br>The homologue recombination in Kluyveromyces lactis is not the preferential way used as repair mechanism of DNA double strand, desirable in proteins expression construction dependent on gene-specific integration. In order to obtain K. lactis strains to recombinant protein expression efficient in homologue recombination way, KU80 gene was interrupted. The perfect running of non-homologue ends junction
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P'Kla, Abalo Di Benedetto Hervé. "Caractérisation en compression simple des blocs de terre comprimée (BTC) application aux maçonneries "BTC-Mortier de terre /." Villeurbanne : Doc'INSA, 2004. http://docinsa.insa-lyon.fr/these/2002/pkla/index.html.

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