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Journal articles on the topic 'PKM1'

Author: Grafiati

Published: 7 September 2023

Last updated: 7 July 2024

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1

Li, Lin, Siyuan Cheng, and Xiuping Yu. "The expression of PKM1 and PKM2 in benign and cancerous prostatic tissues." Journal of Clinical Oncology 41, no. 16_suppl (2023): e17058-e17058. http://dx.doi.org/10.1200/jco.2023.41.16_suppl.e17058.

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e17058 Background: Prostate cancer (PCa) is the most diagnosed cancers in American men. After androgen deprivation therapy (ADT) fails, there is an increase in the aggressive neuroendocrine (NE) phenotype. Currently, there is no effective treatment for NE prostate cancer (NEPC). The metabolic reprogramming, one of the cancer hallmarks, regulates PCa progression and therapy resistance. However, the energy metabolism in NEPC has not been well studied yet. Pyruvate kinase (PK), catalyzing the final step of glycolysis, has PKM1, PKM2, PKL and PKR four isoforms. PKL and PKR are expressed in the liv

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Kim, Seong Ho, Ji Hun Wi, HyeRan Gwak, Eun Gyeong Yang, and So Yeon Kim. "Single-Cell FISH Analysis Reveals Distinct Shifts in PKM Isoform Populations during Drug Resistance Acquisition." Biomolecules 12, no. 8 (2022): 1082. http://dx.doi.org/10.3390/biom12081082.

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The Warburg effect, i.e., the utilization of glycolysis under aerobic conditions, is recognized as a survival advantage of cancer cells. However, how the glycolytic activity is affected during drug resistance acquisition has not been explored at single-cell resolution. Because the relative ratio of the splicing isoform of pyruvate kinase M (PKM), PKM2/PKM1, can be used to estimate glycolytic activity, we utilized a single-molecule fluorescence in situ hybridization (SM-FISH) method to simultaneously quantify the mRNA levels of PKM1 and PKM2. Treatment of HCT116 cells with gefitinib (GE) result

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3

Buneeva, Olga, Arthur Kopylov, Oksana Gnedenko, et al. "Proteomic Profiling of Mouse Brain Pyruvate Kinase Binding Proteins: A Hint for Moonlighting Functions of PKM1?" International Journal of Molecular Sciences 24, no. 8 (2023): 7634. http://dx.doi.org/10.3390/ijms24087634.

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Affinity-based proteomic profiling is widely used for the identification of proteins involved in the formation of various interactomes. Since protein–protein interactions (PPIs) reflect the role of particular proteins in the cell, identification of interaction partners for a protein of interest can reveal its function. The latter is especially important for the characterization of multifunctional proteins, which can play different roles in the cell. Pyruvate kinase (PK), a classical glycolytic enzyme catalyzing the last step of glycolysis, exists in four isoforms: PKM1, PKM2, PKL, and PKR. The

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Verbrugge, Sander A. J., Sebastian Gehlert, Lian E. M. Stadhouders, et al. "PKM2 Determines Myofiber Hypertrophy In Vitro and Increases in Response to Resistance Exercise in Human Skeletal Muscle." International Journal of Molecular Sciences 21, no. 19 (2020): 7062. http://dx.doi.org/10.3390/ijms21197062.

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Nearly 100 years ago, Otto Warburg investigated the metabolism of growing tissues and discovered that tumors reprogram their metabolism. It is poorly understood whether and how hypertrophying muscle, another growing tissue, reprograms its metabolism too. Here, we studied pyruvate kinase muscle (PKM), which can be spliced into two isoforms (PKM1, PKM2). This is of interest, because PKM2 redirects glycolytic flux towards biosynthetic pathways, which might contribute to muscle hypertrophy too. We first investigated whether resistance exercise changes PKM isoform expression in growing human skelet

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Kurihara-Shimomura, Miyako, Tomonori Sasahira, Chie Nakashima, Hiroki Kuniyasu, Hiroyuki Shimomura, and Tadaaki Kirita. "The Multifarious Functions of Pyruvate Kinase M2 in Oral Cancer Cells." International Journal of Molecular Sciences 19, no. 10 (2018): 2907. http://dx.doi.org/10.3390/ijms19102907.

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Head and neck cancers, including oral squamous cell carcinoma (OSCC), are the sixth most common malignancies worldwide. OSCC frequently leads to oral dysfunction, which worsens a patient’s quality of life. Moreover, its prognosis remains poor. Unlike normal cells, tumor cells preferentially metabolize glucose by aerobic glycolysis. Pyruvate kinase (PK) catalyzes the final step in glycolysis, and the transition from PKM1 to PKM2 is observed in many cancer cells. However, little is known about PKM expression and function in OSCC. In this study, we investigated the expression of PKM in OSCC speci

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Li, Qinfeng, Chao Li, Abdallah Elnwasany, et al. "PKM1 Exerts Critical Roles in Cardiac Remodeling Under Pressure Overload in the Heart." Circulation 144, no. 9 (2021): 712–27. http://dx.doi.org/10.1161/circulationaha.121.054885.

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Background: Metabolic remodeling precedes most alterations during cardiac hypertrophic growth under hemodynamic stress. The elevation of glucose utilization has been recognized as a hallmark of metabolic remodeling. However, its role in cardiac hypertrophic growth and heart failure in response to pressure overload remains to be fully illustrated. Here, we aimed to dissect the role of cardiac PKM1 (pyruvate kinase muscle isozyme 1) in glucose metabolic regulation and cardiac response under pressure overload. Methods: Cardiac-specific deletion of PKM1 was achieved by crossing the floxed PKM1 mou

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Kuranaga, Yuki, Nobuhiko Sugito, Haruka Shinohara, et al. "SRSF3, a Splicer of the PKM Gene, Regulates Cell Growth and Maintenance of Cancer-Specific Energy Metabolism in Colon Cancer Cells." International Journal of Molecular Sciences 19, no. 10 (2018): 3012. http://dx.doi.org/10.3390/ijms19103012.

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Serine and arginine rich splicing factor 3 (SRSF3), an SR-rich family protein, has an oncogenic function in various kinds of cancer. However, the detailed mechanism of the function had not been previously clarified. Here, we showed that the SRSF3 splicer regulated the expression profile of the pyruvate kinase, which is one of the rate-limiting enzymes in glycolysis. Most cancer cells express pyruvate kinase muscle 2 (PKM2) dominantly to maintain a glycolysis-dominant energy metabolism. Overexpression of SRSF3, as well as that of another splicer, polypyrimidine tract binding protein 1 (PTBP1) a

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8

Grant, Melissa M. "Pyruvate Kinase, Inflammation and Periodontal Disease." Pathogens 10, no. 7 (2021): 784. http://dx.doi.org/10.3390/pathogens10070784.

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Pyruvate kinase (PK) is the final and rate-limiting enzyme in glycolysis. It has four isoforms PKM1, PKM2, PKL and PKR. PK can form homo tetramers, dimers or monomers. The tetrameric form has the most catalytic activity; however, the dimeric form has non-canonical functions that contribute to the inflammatory response, wound healing and cellular crosstalk. This brief review explores these functions and speculates on their role in periodontal disease.

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Williams, Allison Lesher, Vedbar Khadka, Mingxin Tang, et al. "HIF1 mediates a switch in pyruvate kinase isoforms after myocardial infarction." Physiological Genomics 50, no. 7 (2018): 479–94. http://dx.doi.org/10.1152/physiolgenomics.00130.2017.

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Alternative splicing of RNA is an underexplored area of transcriptional response. We expect that early changes in alternatively spliced genes may be important for responses to cardiac injury. Hypoxia inducible factor 1 (HIF1) is a key transcription factor that rapidly responds to loss of oxygen through alteration of metabolism and angiogenesis. The goal of this study was to investigate the transcriptional response after myocardial infarction (MI) and to identify novel, hypoxia-driven changes, including alternative splicing. After ligation of the left anterior descending artery in mice, we obse

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Lee, Yuumi, Yuko Ito, Kohei Taniguchi, Takashi Nuri, SangWoong Lee, and Koichi Ueda. "Experimental Study of Warburg Effect in Keloid Nodules: Implication for Downregulation of miR-133b." Plastic and Reconstructive Surgery - Global Open 11, no. 8 (2023): e5202. http://dx.doi.org/10.1097/gox.0000000000005202.

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Background: A keloid is composed of several nodules, which are divided into two zones: the central zone (CZ; a hypoxic region) and the marginal zone (MZ; a normoxic region). Keloid nodules play a key role in energy metabolic activity for continuous growth by increasing in number and total area. In this study, we aimed to investigate the roles of the zones in the execution of the Warburg effect and identify which microRNAs regulate this phenomenon in keloid tissue. Methods: Eleven keloids from patients were used. Using immunohistochemical analysis, 179 nodules were randomly chosen from these ke

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Mojzeš, Anamarija, Marko Tomljanović, Lidija Milković, Renata Novak Kujundžić, Ana Čipak Gašparović, and Koraljka Gall Trošelj. "Cell-Type Specific Metabolic Response of Cancer Cells to Curcumin." International Journal of Molecular Sciences 21, no. 5 (2020): 1661. http://dx.doi.org/10.3390/ijms21051661.

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In order to support uncontrolled proliferation, cancer cells need to adapt to increased energetic and biosynthetic requirements. One such adjustment is aerobic glycolysis or the Warburg effect. It is characterized by increased glucose uptake and lactate production. Curcumin, a natural compound, has been shown to interact with multiple molecules and signaling pathways in cancer cells, including those relevant for cell metabolism. The effect of curcumin and its solvent, ethanol, was explored on four different cancer cell lines, in which the Warburg effect varied. Vital cellular parameters (proli

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Ikezoe, Takayuki, Rongqing Li, Na Sun, Xin Chen, and Jing Yang. "JAK2V617F expressing macrophages stimulate IL-6 production through PKM1 mediated STAT3 activation." Journal of Immunology 204, no. 1_Supplement (2020): 59.18. http://dx.doi.org/10.4049/jimmunol.204.supp.59.18.

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Abstract The JAK2V617F mutation is intimately involved in the pathogenesis of myeloproliferative neoplasms (MPNs) including polycythemia vera and essential thrombocytosis. The thrombotic events are serious complications occurring in individuals with MPNs which affect their prognosis. Interestingly, JAK2V617F is detected in some individuals who developed thrombosis without MPNs and thrombotic risk factors. These observations prompted us to hypothesize that JAK2V617F arising in myeloid cells could contribute to cardiovascular disease by promoting the production of proinflammatory cytokines. To t

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da Silva, Esdras Matheus Gomes, Letícia Graziela Costa Santos, Flávia Santiago de Oliveira, et al. "Proteogenomics Reveals Orthologous Alternatively Spliced Proteoforms in the Same Human and Mouse Brain Regions with Differential Abundance in an Alzheimer’s Disease Mouse Model." Cells 10, no. 7 (2021): 1583. http://dx.doi.org/10.3390/cells10071583.

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Alternative splicing (AS) may increase the number of proteoforms produced by a gene. Alzheimer’s disease (AD) is a neurodegenerative disease with well-characterized AS proteoforms. In this study, we used a proteogenomics strategy to build a customized protein sequence database and identify orthologous AS proteoforms between humans and mice on publicly available shotgun proteomics (MS/MS) data of the corpus callosum (CC) and olfactory bulb (OB). Identical proteotypic peptides of six orthologous AS proteoforms were found in both species: PKM1 (gene PKM/Pkm), STXBP1a (gene STXBP1/Stxbp1), Isoform

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Oluwaseunfunmi Samuel, Olaborode, Gardner Cassel Samuel, and Onokpise Oghenekome Urakpo. "Growth performance of selected Moringa oleifera seed origins in North Florida." Journal of Plant Science and Phytopathology 6, no. 1 (2022): 001–7. http://dx.doi.org/10.29328/journal.jpsp.1001066.

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Moringa oleifera which is also known as horseradish or drumstick tree is a rapidly growing, drought-tolerant tree that can tolerate poor soil conditions. It is presently broadly cultivated and has turned out to be naturalized in numerous geographical areas of the tropics. The objective of this study was to evaluate the growth performance and survivability of Moringa seed origins in the North Florida region of the United States of America. Seedlings were prepared in the George Conoly Greenhouse at the Florida A&M University, Tallahassee, FL. The seed origins were Ghana, Texas PKM1, Haiti PK

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Sun, Ye, Yushi Chen, Ming Xu, Chunying Liu, Hai Shang, and Chun Wang. "Shenmai Injection Supresses Glycolysis and Enhances Cisplatin Cytotoxicity in Cisplatin-Resistant A549/DDP Cells via the AKT-mTOR-c-Myc Signaling Pathway." BioMed Research International 2020 (June 22, 2020): 1–10. http://dx.doi.org/10.1155/2020/9243681.

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Tumor cells, especially drug-resistant cells, predominately support growth by glycolysis even under the condition of adequate oxygen, which is known as the Warburg effect. Glucose metabolism reprogramming is one of the main factors causing tumor resistance. Previous studies on Shenmai injection (SMI), a Chinese herbal medicine, have shown enhanced efficacy in the treatment of tumors in combination with chemotherapy drugs, but the mechanism is not clear. In this study, we investigated the effect of SMI combined with cisplatin on cisplatin-resistant lung adenocarcinoma A549/DDP cells. Our result

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Huang, Rong, Rong Han, Yucheng Yan, et al. "PTB Regulates the Metabolic Pathways and Cell Function of Keloid Fibroblasts through Alternative Splicing of PKM." International Journal of Molecular Sciences 24, no. 6 (2023): 5162. http://dx.doi.org/10.3390/ijms24065162.

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Keloids, benign fibroproliferative cutaneous lesions, are characterized by abnormal growth and reprogramming of the metabolism of keloid fibroblasts (KFb). However, the underlying mechanisms of this kind of metabolic abnormality have not been identified. Our study aimed to investigate the molecules involved in aerobic glycolysis and its exact regulatory mechanisms in KFb. We discovered that polypyrimidine tract binding (PTB) was significantly upregulated in keloid tissues. siRNA silencing of PTB decreased the mRNA levels and protein expression levels of key glycolytic enzymes and corrected the

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17

Goldberg, Michael S., and Phillip A. Sharp. "Pyruvate kinase M2-specific siRNA induces apoptosis and tumor regression." Journal of Experimental Medicine 209, no. 2 (2012): 217–24. http://dx.doi.org/10.1084/jem.20111487.

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The development of cancer-specific therapeutics has been limited because most healthy cells and cancer cells depend on common pathways. Pyruvate kinase (PK) exists in M1 (PKM1) and M2 (PKM2) isoforms. PKM2, whose expression in cancer cells results in aerobic glycolysis and is suggested to bestow a selective growth advantage, is a promising target. Because many oncogenes impart a common alteration in cell metabolism, inhibition of the M2 isoform might be of broad applicability. We show that several small interfering (si) RNAs designed to target mismatches between the M2 and M1 isoforms confer s

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Ensink, Elliot, and Sophia Y. Lunt. "Abstract 3031: Impact of pyruvate kinase isoforms on cysteine metabolism in pancreatic cancer." Cancer Research 82, no. 12_Supplement (2022): 3031. http://dx.doi.org/10.1158/1538-7445.am2022-3031.

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Abstract Pancreatic cancer cells undergo metabolic alterations to survive within a nutrient-depleted tumor microenvironment. Cysteine metabolism in particular is critical for supporting pancreatic cancer cell survival through its role in redox homeostasis. Targeting cysteine metabolism presents an underexplored opportunity for therapeutic exploitation, but much remains unknown about how cysteine metabolism is influenced by microenvironmental conditions and the overall metabolic state of the cell. One critical metabolic shift in pancreatic cancer cells occurs through altered isoform expression

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Akbar Napitupulu, Alief, Ni Nyoman Puspawati, and I. Desak Putu Kartika Pratiwi. "CEMARAN ASPERGILLUS FLAVUS PENGHASIL AFLATOKSIN B1 PADA JAGUNG MANIS (ZEA MAYS SACCARATA) YANG DIJUAL DI PASAR TRADISIONAL DAN PASAR MODERN DI KECAMATAN DENPASAR BARAT, KOTA MADYA DENPASAR – BALI." Jurnal Ilmu dan Teknologi Pangan (ITEPA) 7, no. 2 (2018): 11. http://dx.doi.org/10.24843/itepa.2018.v07.i02.p02.

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   This study aims to determine contamination, Aspergillus flavus in sweet corn sold in traditional and modern markets in West Denpasar district and to know whether A. flavus contaminating sweet corn can produce aflatoxin B1. The design of this study applied survey methods with sampling techniques of purposive sampling. Sampling determination was conducted on a number of markets, which were Traditional Markets and Modern Markets in West Denpasar District. West Denpasar District has 7 traditional markets and 3 modern markets. The variables observed were total mold of yeast, to

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Xia, Yong, Xing Wang, Yan Liu, et al. "PKM2 Is Essential for Bladder Cancer Growth and Maintenance." Cancer Research 82, no. 4 (2021): 571–85. http://dx.doi.org/10.1158/0008-5472.can-21-0403.

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Abstract Pyruvate kinase M2 (PKM2) has been shown to promote tumorigenesis by facilitating the Warburg effect and enhancing the activities of oncoproteins. However, this paradigm has recently been challenged by studies in which the absence of PKM2 failed to inhibit and instead accelerated tumorigenesis in mouse models. These results seem inconsistent with the fact that most human tumors overexpress PKM2. To further elucidate the role of PKM2 in tumorigenesis, we investigated the effect of PKM2 knockout in oncogenic HRAS-driven urothelial carcinoma. While PKM2 ablation in mouse urothelial cells

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Warenius, Hilmar M. "Selectively depleting the energy of cancer cells: A new therapeutic paradigm." Journal of Cancer Biology 5, no. 1 (2024): 1–10. http://dx.doi.org/10.46439/cancerbiology.5.057.

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Over the past fifty years the somatic mutation theory of cancer has emerged as the most successful explanation of the molecular phenotype of human cancer cells. Normal non-mutated genes may, however, also play a role in carcinogenesis. In particular these may contribute to aerobic glycolysis and the potential interaction of PKM2 and Cdk4 in helping the nascent cancer cell avoid apoptosis by the interaction of their respective amino acid sequences: anionic SDPTEA and cationic PRGPRP. It is proposed that cancer first occurs in normal tissues cells which, as part of the premalignant phenotype hav

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Bluemlein, Katharina, Nana-Maria Grüning, René G. Feichtinger, Hans Lehrach, Barbara Kofler, and Markus Ralser. "No evidence for a shift in pyruvate kinase PKM1 to PKM2 expression during tumorigenesis." Oncotarget 2, no. 5 (2011): 393–400. http://dx.doi.org/10.18632/oncotarget.278.

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Lemos, Fernanda O., Ian de Ridder, Martin D. Bootman, Geert Bultynck, and Jan B. Parys. "The Complex Effects of PKM2 and PKM2:IP3R Disruption on Intracellular Ca2+ Handling and Cellular Functions." Cells 12, no. 21 (2023): 2527. http://dx.doi.org/10.3390/cells12212527.

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Pyruvate kinase M (PKM) 2 was described to interact with the inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) and suppress its activity. To further investigate the physiological importance of the PKM2:IP3R interaction, we developed and characterized HeLa PKM2 knockout (KO) cells. In the HeLa PKM2 KO cells, the release of Ca2+ to the cytosol appears to be more sensitive to low agonist concentrations than in HeLa wild-type (WT) cells. However, upon an identical IP3-induced Ca2+ release, Ca2+ uptake in the mitochondria is decreased in HeLa PKM2 KO cells, which may be explained by the smaller nu

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Nayak, Manasa, Nirav Dhanesha, Manish Jain, and Anil Chauhan. "Manipulating Metabolic Plasticity By Targeting Pyruvate Kinase M2 in Platelets Inhibits Arterial Thrombosis." Blood 132, Supplement 1 (2018): 868. http://dx.doi.org/10.1182/blood-2018-99-112704.

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Abstract Background: Most of the cellular responses initiated upon platelet activation are energy consuming. Like normal cells, resting platelets rely primarily on oxidative phosphorylation (OXPHOS) to generate ATP, whereas activated platelets exhibit a high level of aerobic glycolysis (conversion of glucose to lactate in the presence of oxygen, a phenomenon referred to as the Warburg effect in tumor cells) suggesting that metabolic plasticity exists in activated platelets. Although aerobic glycolysis yields less total ATP when compared to OXPHOS, the rate of ATP generation is faster in aerobi

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Taniguchi, Kohei, Nobuhiko Sugito, Minami Kumazaki, et al. "MicroRNA-124 inhibits cancer cell growth through PTB1/PKM1/PKM2 feedback cascade in colorectal cancer." Cancer Letters 363, no. 1 (2015): 17–27. http://dx.doi.org/10.1016/j.canlet.2015.03.026.

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Tariq, Sadaf, Huma Umbreen, Razia Noreen, et al. "Comparative Analysis of Antioxidants Activity of Indigenously Produced Moringa Oleifera Seeds Extracts." BioMed Research International 2022 (October 15, 2022): 1–11. http://dx.doi.org/10.1155/2022/4987929.

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Medicinal plants are used to control and remediate oxidative stress related diseases caused by free radicals. Thus, these plants find their use as remedy. Moringa oleifera is an extremely valued plant for its medicinal properties. Herein, two indigenously produced accessions of Moringa oleifera seeds [originated from Multan (M-Mln) and India (PKM1)] were investigated for their antioxidant properties by 2.2-Diphenyl-1picrylhydrazyl (DPPH) assay, total phenolics content and total flavonoids content. The presence of various phenolics as well as flavonoids was further confirmed by high performance

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Bartyś, Natalia, Anna Pasternak, and Jolanta Lisowiec-Wąchnicka. "Optimization of Bifunctional Antisense Oligonucleotides for Regulation of Mutually Exclusive Alternative Splicing of PKM Gene." Molecules 27, no. 17 (2022): 5682. http://dx.doi.org/10.3390/molecules27175682.

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Oligonucleotide tools, as modulators of alternative splicing, have been extensively studied, giving a rise to new therapeutic approaches. In this article, we report detailed research on the optimization of bifunctional antisense oligonucleotides (BASOs), which are targeted towards interactions with hnRNP A1 protein. We performed a binding screening assay, Kd determination, and UV melting experiments to select sequences that can be used as a high potency binding platform for hnRNP A1. Newly designed BASOs were applied to regulate the mutually exclusive alternative splicing of the PKM gene. Our

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Yan, Qiuxia, Peng Zeng, Xiuqin Zhou, et al. "RBMX suppresses tumorigenicity and progression of bladder cancer by interacting with the hnRNP A1 protein to regulate PKM alternative splicing." Oncogene 40, no. 15 (2021): 2635–50. http://dx.doi.org/10.1038/s41388-021-01666-z.

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AbstractThe prognosis for patients with metastatic bladder cancer (BCa) is poor, and it is not improved by current treatments. RNA-binding motif protein X-linked (RBMX) are involved in the regulation of the malignant progression of various tumors. However, the role of RBMX in BCa tumorigenicity and progression remains unclear. In this study, we found that RBMX was significantly downregulated in BCa tissues, especially in muscle-invasive BCa tissues. RBMX expression was negatively correlated with tumor stage, histological grade and poor patient prognosis. Functional assays demonstrated that RBM

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Shi, Weiye, Xu Yao, Xueyu Cao, Yu Fu, and Yingze Wang. "Serine/Arginine-Rich Splicing Factor 7 Knockdown Inhibits Aerobic Glycolysis and Growth in HepG2 Cells by Regulating PKM2 Expression." Current Issues in Molecular Biology 46, no. 5 (2024): 5023–36. http://dx.doi.org/10.3390/cimb46050301.

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Serine/arginine-rich splicing factors (SRSFs), part of the serine/arginine-rich (SR) protein family, play a crucial role in precursor RNA splicing. Abnormal expression of SRSFs in tumors can disrupt normal RNA splicing, contributing to tumor progression. Notably, SRSF7 has been found to be upregulated in hepatocellular carcinoma (HCC), yet its specific role and molecular mechanisms in HCC pathogenesis are not fully understood. We investigated the expression and prognostic significance of SRSF7 in HCC using bioinformatics database analysis. In HepG2 cells, the expressions of SRSF7 and glycolyti

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Singh, Smriti, Sathiya Pandi Narayanan, Kajal Biswas, et al. "Intragenic DNA methylation and BORIS-mediated cancer-specific splicing contribute to the Warburg effect." Proceedings of the National Academy of Sciences 114, no. 43 (2017): 11440–45. http://dx.doi.org/10.1073/pnas.1708447114.

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Aberrant alternative splicing and epigenetic changes are both associated with various cancers, but epigenetic regulation of alternative splicing in cancer is largely unknown. Here we report that the intragenic DNA methylation-mediated binding of Brother of Regulator of Imprinted Sites (BORIS) at the alternative exon of Pyruvate Kinase (PKM) is associated with cancer-specific splicing that promotes the Warburg effect and breast cancer progression. Interestingly, the inhibition of DNA methylation, BORIS depletion, or CRISPR/Cas9-mediated deletion of the BORIS binding site leads to a splicing swi

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Chiavarina, Barbara, Diana Whitaker-Menezes, Ubaldo E. Martinez-Outschoorn, et al. "Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth." Cancer Biology & Therapy 12, no. 12 (2011): 1101–13. http://dx.doi.org/10.4161/cbt.12.12.18703.

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Taniguchi, Kohei, Miku Sakai, Nobuhiko Sugito, et al. "PKM1 is involved in resistance to anti-cancer drugs." Biochemical and Biophysical Research Communications 473, no. 1 (2016): 174–80. http://dx.doi.org/10.1016/j.bbrc.2016.03.074.

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Li, Yuchao, Shuwei Zhang, Yuexian Li, et al. "The Regulatory Network of hnRNPs Underlying Regulating PKM Alternative Splicing in Tumor Progression." Biomolecules 14, no. 5 (2024): 566. http://dx.doi.org/10.3390/biom14050566.

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One of the hallmarks of cancer is metabolic reprogramming in tumor cells, and aerobic glycolysis is the primary mechanism by which glucose is quickly transformed into lactate. As one of the primary rate-limiting enzymes, pyruvate kinase (PK) M is engaged in the last phase of aerobic glycolysis. Alternative splicing is a crucial mechanism for protein diversity, and it promotes PKM precursor mRNA splicing to produce PKM2 dominance, resulting in low PKM1 expression. Specific splicing isoforms are produced in various tissues or illness situations, and the post-translational modifications are linke

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Balakumbahan, R., C. Ravindran, and V. Sivakumar. "Standardization of Propagation Techniques in Annual Moringa (Moringa oleifera Lam.) for Enhancing Crop Uniformity." Journal of Advances in Biology & Biotechnology 27, no. 7 (2024): 1315–20. http://dx.doi.org/10.9734/jabb/2024/v27i71093.

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An investigation on annual Moringa, Moringa oleifera Lam., var., (PKM 1 and PKM2) with the objective of standardizing the method of vegetative propagation was carried out at Horticultural College and Research Institute, Periyakulam. the treatment consisted of three factors viz., cultivars (Factor 1: Moringa variety PKM 1 and PKM2), propagation methods (Factor 2: hardwood cuttings, semi hardwood cuttings and air layering) and application of different concentration of growth regulator IBA (Factor 3:0, 1500, 2500 and 4000 ppm) and the trial was laid out with twenty four treatments using Factorial

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Hu, Zhuozhou, Jing Zhou, Liang Han, et al. "Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice." Life Sciences 304 (September 2022): 120725. http://dx.doi.org/10.1016/j.lfs.2022.120725.

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Dogsom, Oyungerel, Amir Hamza, Shohel Mahmud, Jung-Ki Min, Yoon-Beom Lee, and Jae-Bong Park. "The Complex of p-Tyr42 RhoA and p-p65/RelA in Response to LPS Regulates the Expression of Phosphoglycerate Kinase 1." Antioxidants 12, no. 12 (2023): 2090. http://dx.doi.org/10.3390/antiox12122090.

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Inflammation plays a crucial role in tumorigenesis, primarily mediated by NF-κB. RhoA GTPases are instrumental in regulating the activation of NF-κB. Specifically, the phosphorylation of Tyrosine 42 on RhoA ensures the activation of NF-κB by directly activating the IKKβ associated with IKKγ (NEMO). This study aimed to uncover the molecular mechanism through which p-Tyrosine 42 RhoA, in conjunction with NF-κB, promotes tumorigenesis. Notably, we observed that p-Tyrosine 42 RhoA co-immunoprecipitated with the p-Ser 536 p65/RelA subunit in NF-κB in response to LPS. Moreover, both p-Tyrosine 42 Rh

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Wang, Ying-Hua, William J. Israelsen, Dongjun Lee, et al. "Differential Dependence On Aerobic Glycolysis In Normal and Malignant Hematopoietic Stem and Progenitor Cells To Sustain Daughter Cell Production." Blood 122, no. 21 (2013): 793. http://dx.doi.org/10.1182/blood.v122.21.793.793.

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Abstract How glucose is metabolized can influence cell function, but whether differences in glucose metabolism reflect, or dictate, cell state is not clear and is of particular interest given the association of cancer with aerobic glycolysis. Studies on cancer cell lines have indicated that increased glucose uptake with lactate production regardless of oxygen concentration, a phenomenon also known as the Warburg effect, is promoted in part by expression of the M2 isoform of pyruvate kinase (PKM2) and the muscle form of lactate dehydrogenase A (LDHA). Normal somatic cells thought to also prefer

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FOSTER, HANNAH R., THUONG HO, EVGENIY POTAPENKO, et al. "127-OR: In Vivo Genetic Evidence That the Pyruvate Kinase Isoforms PKM1 and PKM2 Differentially Control Beta-Cell Fuel Sensing." Diabetes 70, Supplement 1 (2021): 127—OR. http://dx.doi.org/10.2337/db21-127-or.

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T. Surendhar et al.,, T. Surendhar et al ,. "Tamarind Seed Gum as Adhesive for Pelleting in Tomato Cv. PKM1." International Journal of Agricultural Science and Research 9, no. 3 (2019): 201–8. http://dx.doi.org/10.24247/ijasrjun201931.

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Morita, Mami, Taku Sato, Miyuki Nomura, et al. "PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth." Cancer Cell 33, no. 3 (2018): 355–67. http://dx.doi.org/10.1016/j.ccell.2018.02.004.

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Méndez-Lucas, Andrés, Xiaolei Li, Junjie Hu, et al. "Glucose Catabolism in Liver Tumors Induced by c-MYC Can Be Sustained by Various PKM1/PKM2 Ratios and Pyruvate Kinase Activities." Cancer Research 77, no. 16 (2017): 4355–64. http://dx.doi.org/10.1158/0008-5472.can-17-0498.

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Nayak, Manasa K., Madankumar Ghatge, Nirav Dhanesha, et al. "Targeting Metabolic Enzyme Pyruvate Kinase M2: A Novel Strategy to Inhibit Platelet Function and Arterial Thrombosis." Blood 134, Supplement_1 (2019): 1056. http://dx.doi.org/10.1182/blood-2019-129027.

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Background: The cellular responses initiated upon platelet activation are energy consuming. Activated platelets, in comparison to their resting state, exhibit a high level of aerobic glycolysis (conversion of glucose to lactate in the presence of oxygen) relative to oxidative phosphorylation (OXPHOS), suggesting that metabolic plasticity exists in platelets. Although aerobic glycolysis yields less total ATP when compared to OXPHOS, the rate of ATP generation is faster in aerobic glycolysis compared to OXPHOS, which we hypothesize is well suited for high-energy requirement during platelet activ

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Li, Dongliang, Yan Xie, Jisan Sun, Li Zhang, and Wentao Jiang. "LncRNA ZNF674-AS1 Hinders Proliferation and Invasion of Hepatic Carcinoma Cells through the Glycolysis Pathway." Journal of Oncology 2022 (July 13, 2022): 1–9. http://dx.doi.org/10.1155/2022/8063382.

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Purpose. Long noncoding RNAs (lncRNAs) play important roles in regulating various functions of cells at the levels of transcription and posttranscription. Extensive investigations have illustrated that lncRNAs are critical in the glucose metabolism of tumor cells, but their mechanisms of action need to be further explored. This study evaluates the role of lncRNA ZNF674-AS1 on the apoptosis and proliferation of human hepatic carcinoma cells in vitro through the glucose metabolism and its related mechanisms. Methods. Real-time quantitative PCR was employed for detecting the level of expressions

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Okazaki, Mitsuyoshi, Sachio Fushida, Tomoya Tsukada, et al. "The effect of HIF-1α and PKM1 expression on acquisition of chemoresistance." Cancer Management and Research Volume 10 (July 2018): 1865–74. http://dx.doi.org/10.2147/cmar.s166136.

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Li, Qi, Xin Qi, and Wenjun Jia. "3,3′,5-triiodothyroxine inhibits apoptosis and oxidative stress by the PKM2/PKM1 ratio during oxygen-glucose deprivation/reperfusion AC16 and HCM-a cells." Biochemical and Biophysical Research Communications 475, no. 1 (2016): 51–56. http://dx.doi.org/10.1016/j.bbrc.2016.05.030.

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Sturgill, Eric M., and Monica L. Guzman. "Cytokine Induced Nuclear Localization Of Pyruvate Kinase M2 In Acute Myeloid Leukemia." Blood 122, no. 21 (2013): 5406. http://dx.doi.org/10.1182/blood.v122.21.5406.5406.

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Abstract A common characteristic among nearly all cancers, including leukemia, is the cell’s metabolic proclivity for glycolysis over the more energy efficient process of oxidative phosphorylation (OXPHOS) in the presence of oxygen. This altered state of aerobic glycolysis was observed in tumor cells by Otto Warburg over fifty years ago (Warburg, 1956) and continues to be intensely investigated in hopes of ultimately exploiting this “Warburg effect” in the treatment of cancer (Vander Heiden et al. 2009). Recent studies have revealed that the M2 isoform of the enzyme pyruvate kinase (PKM2) play

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Inagaki, Maiko, Tobias Schmelzle, Kyoko Yamaguchi, Kenji Irie, Michael N. Hall, and Kunihiro Matsumoto. "PDK1 Homologs Activate the Pkc1–Mitogen-Activated Protein Kinase Pathway in Yeast." Molecular and Cellular Biology 19, no. 12 (1999): 8344–52. http://dx.doi.org/10.1128/mcb.19.12.8344.

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ABSTRACT PDK1 (phosphoinositide-dependent kinase 1) is a mammalian growth factor-regulated serine/threonine kinase. Using a genetic selection based on a mutant form of the yeast MAP kinase kinase Ste7, we isolated a gene, PKH2, encoding a structurally and functionally conserved yeast homolog of PDK1. Yeast cells lacking bothPKH2 and PKH1, encoding another PDK1 homolog, were nonviable, indicating that Pkh1 and Pkh2 share an essential function. A temperature-sensitive mutant, pkh1D398Gpkh2, was phenotypically similar to mutants defective in the Pkc1–mitogen-activated protein kinase (MAPK) pathwa

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Wei, Libin, Yuanyuan Dai, Yuxin Zhou, et al. "Oroxylin A activates PKM1/HNF4 alpha to induce hepatoma differentiation and block cancer progression." Cell Death & Disease 8, no. 7 (2017): e2944-e2944. http://dx.doi.org/10.1038/cddis.2017.335.

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D'Souza, Cletus A., J. Andrew Alspaugh, Changli Yue, et al. "Cyclic AMP-Dependent Protein Kinase Controls Virulence of the Fungal Pathogen Cryptococcus neoformans." Molecular and Cellular Biology 21, no. 9 (2001): 3179–91. http://dx.doi.org/10.1128/mcb.21.9.3179-3191.2001.

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ABSTRACT Cryptococcus neoformans is an opportunistic fungal pathogen that infects the human central nervous system. This pathogen elaborates two specialized virulence factors: the antioxidant melanin and an antiphagocytic immunosuppressive polysaccharide capsule. A signaling cascade controlling mating and virulence was identified. ThePKA1 gene encoding the major cyclic AMP (cAMP)-dependent protein kinase catalytic subunit was identified and disrupted.pka1 mutant strains were sterile, failed to produce melanin or capsule, and were avirulent. The PKR1 gene encoding the protein kinase A (PKA) reg

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Taniguchi, Kohei, Kazuhisa Uchiyama, and Yukihiro Akao. "PTBP1 ‐targeting microRNAs regulate cancer‐specific energy metabolism through the modulation of PKM1/M2 splicing." Cancer Science 112, no. 1 (2020): 41–50. http://dx.doi.org/10.1111/cas.14694.

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