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Journal articles on the topic 'Placental extract gel'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Sriram, VS, Ravi Kumar Chittoria, and JS Amrutha. "Placental Extract Gel as a Topical Agent for Optimizing Healing of Full-thickness Skin Graft Donor Sites." Archives of Medical Case Reports 7, no. 1 (2025): 11–15. https://doi.org/10.33696/casereports.7.035.

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Full-thickness skin graft (FTSG) donor sites are often closed primarily, particularly when small and located in areas with adequate tissue laxity. Despite primary closure, these sites may still experience delayed healing, discomfort, or hypertrophic scarring. Placental extract gel, derived from human placenta, is rich in growth factors, cytokines, and bioactive peptides known to facilitate wound healing and modulate scar formation through enhanced angiogenesis, anti-inflammatory action, and microbial resistance. Its biocompatibility and regenerative properties make it a promising adjunct in wo

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2

Sakura, Harutake, Shigeru Aoki, Takachika Ozawa, Tadashi Hashimoto, and Naoki Sakura. "The neuropeptide, head activator, in human placenta and serum from pregnant women." Acta Endocrinologica 125, no. 4 (1991): 454–58. http://dx.doi.org/10.1530/acta.0.1250454.

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Abstract. The hydra neuropeptide, head activator, was detected in human placenta using radioimmunoassay. The placenta contained 11-68 fmol/g wet weight of head activator. The slope of the inhibition curve by placental extract in radioimmunoassay was identical to that of synthetic head activator. The molecular weight of the major immunoreactive head activator in placental extract corresponded to the synthetic head activator after Bio-Gel P-2 column chromatography. The peak of immunoreactive head activator in the extract emerged at the same retention time as that of the synthetic head activator

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3

De, Bishnupada, Anupam Baske, Fasahat Hussain, and Partha Mandal. "<b>Assessment of healing effectiveness of topical platelet-derived growth factor versus placental extract in diabetic foot ulcer: A randomized clinical trial in a tertiary care hospital in the eastern zone of India</b>." National Journal of Physiology, Pharmacy and Pharmacology 14, no. 12 (2024): 2504. https://doi.org/10.5455/njppp.2024.v14.i12.1.

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Background: Globally about 370 million people have diabetes mellitus and 25% of total diabetic patients develop a diabetic foot ulcers (DFU) during their lifetime. If early and proper intervention not taken DFU can rapidly deteriorate, may leads to amputation of the affected limb. Human derived placental extract is available in India at affordable cost and knowing it’s safety profile we planned to conduct a prospective study to compare the effectiveness of topical platelet derived growth factor (PDGF) and placental extract in DFU. Aim: The aim of our study was to compare the healing effectiven

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Lokesh, K., and C. Hemanth Mahesh. "To Study the Efficacy of Placental Extract Gel in Chronic Non Healing Foot Ulcer." New Indian Journal of surgery 12, no. 4 (2021): 227–31. http://dx.doi.org/10.21088/nijs.0976.4747.12421.5.

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Wound healing is a complex and regulated process that is critical in maintaining the skin barrier function. The numerous disease processes can affect the events involved in wound healing, resulting in chronic, non-healing wounds. These would subject them to significant discomfort and distress and drain the medical system resources enormously. A chronic wound can be defined as that does not heal in an orderly set of stages and in a predictable amount of time or within three months. They remain in the inflammatory phase for too longand never heal or may take years.3,4 Chronic leg and foot ulcers

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5

Siva, Mandadap, and Javali Sharanabasavaraj. "Outcome of Placental Extract Dressing versus Collagen Sheet Dressing for Partial Thickness Burns: A Comparative Study." International Journal of Pharmaceutical and Clinical Research 14, no. 11 (2022): 253–58. https://doi.org/10.5281/zenodo.13267212.

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<strong>Introduction: </strong>Burns are major morbid health issues with complex pathophysiology. Topical management of burns is a challenging task for surgeon. Effective topical agent should have less duration of healing, less duration of hospitalization, pain reduction, better scar formation and less incidence of infection properties. The present study designed to assess placental extract dressing on collagen sheet dressing for partial thickness burns. <strong>Material and Methods:</strong> Seventy-six cases attended with partial thickness burns (<40%) under 55 years of age

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Sharma, Aakriti, Sanjeev Sharma, and Amit Nagar. "Comparative Evaluation to Assess the Effect of SRP With or Without Human Placental Extracts as Local Drug Delivery in Treatment of Localized Periodontal Pocket- A Randomized Controlled Clinical Trial." International Journal of Dentistry Research 5, no. 2 (2020): 66–70. http://dx.doi.org/10.31254/dentistry.2020.5208.

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Background: Periodontal disease is one of the most prevalent global chronic disorder. Pathology affecting the structure surrounding teeth results in inflammation initiated by bacterial aggregation & alteration in their profile. Conventional periodontal therapeutics has focused on the control of etiologic agents, thereby promoting healing & repair of tissues. Delivery of therapeutic agents into the local milieu act as drug reservoirs which could alter pathogenic flora & promote its repair & wound healing. Aim & Objective: In an effort to develop a novel therapy, the present

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Tiwary, S. K., D. Shukla, A. K. Tripathi, S. Agrawal, M. K. Singh, and V. K. Shukla. "Effect of placental-extract gel and cream on non-healing wounds." Journal of Wound Care 15, no. 7 (2006): 325–28. http://dx.doi.org/10.12968/jowc.2006.15.7.26937.

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8

Kuivaniemi, H. "Partial characterization of lysyl oxidase from several human tissues." Biochemical Journal 230, no. 3 (1985): 639–43. http://dx.doi.org/10.1042/bj2300639.

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Lysyl oxidase activity was assayed in urea extracts of a number of human tissues, proving to be highest in skin. Antibodies to human placental lysyl oxidase completely inhibited the activity of crude lysyl oxidase from all the human tissues studied, with no significant differences in the amounts of antiserum required for 50% inhibition. By contrast, marked differences were found in this value between skin tissue samples from different species. The Mr of lysyl oxidase in crude extracts of human skin and in the medium of cultured human skin fibroblasts was 30 000 by gel filtration, no active spe

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9

N, Lkhagvasuren, Bayar-Enkh B, Sainbileg G, et al. "Study of the anti-inflammatory and immunomodulatory activity of bovine placental extract." Mongolian Journal of Agricultural Sciences 28, no. 03 (2019): 15–25. http://dx.doi.org/10.5564/mjas.v28i03.1297.

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Many scientists study structure and charagterization of placenta and experimenting on their biological activity, due to develop new drugs and biopreparation for human and animal health. The present study aimed to isolate bovine placental lactogen from placenta by semi purification method and determine antioxidant, anti-inflamatory activity and effect on immune responses was also investigation. Semi purified protein was separated by gel-chromatographic method using 3 methods of tissue homogenizations of placental tissue using distilled water, phosphate buffer saline and Tris-HCl, by use o

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10

S., Umamaheshwara Raju, T. S. Usha Sree, Karuna Sree Podila, and Vijay Krishna. "Comparative study of topical application of nanosilver and human placental extract on wound healing in rabbits." International Journal of Basic & Clinical Pharmacology 6, no. 8 (2017): 1926. http://dx.doi.org/10.18203/2319-2003.ijbcp20173272.

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Background: The objective of the study was to study the comparative effect of topical application of nanosilver and human placental extract on wound healing in rabbits.Methods: 12 rabbits were randomly divided into two groups (n=6) and wound healing effect was observed in Excision model. Standard group was treated with topical human placental extract gel and test group was treated with topical Nanosilver cream. The mean percentage of the wound healed ‘within’ the group and ‘in between’ the groups were observed on day 3, 6, 9, 12, 15, 18, 21 and biopsy was done on day 21 for histopathological e

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11

Alvaro, Ruben E., May Robertson, Saad Al-Saedi, Robert P. Lemke, Don B. Cates, and Henrique Rigatto. "Preliminary characterization of a placental factor inhibiting breathing in fetal sheep." Reproduction, Fertility and Development 9, no. 6 (1997): 641. http://dx.doi.org/10.1071/r97031.

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Previous studies have revealed a placental extract that inhibits breathing in fetal sheep. In the present study of 29 chronically instrumented sheep at 132±1 days of gestation, infusion of the 1-10 kDa extract inhibited breathing in 76% of the experiments whereas Krebs’ solution inhibited it in 24%. It retained this activity after 6 months of freezing, after lyophilization, and upon lowering the pH during purication from 8·0 to 4·0, but it inhibited breathing in only 35% when the pH was lowered to 2·0. A signicant dose-dependent effect was observed from a 16-fold dilution to a 4-fold concentra

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12

D, Batsaikhan, Lkhagvasuren N, Bayar-Enkh B, et al. "Result of determination placental protein 13 (pp13) and placental growth factor in placental extract of mongolian mares." Mongolian Journal of Agricultural Sciences 25, no. 03 (2018): 57–65. http://dx.doi.org/10.5564/mjas.v25i03.1172.

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The present study aimed to isolate some proteins from placenta of Mongolian mares and measure their concentrations using ELISA test kits. In order to achieve this aim, a total of 12 variants of protein isolation experiments in triplicates were performed to isolate proteins from each homogenates, which were obtained with 3 methods of tissue homogenizations of placental tissue of Mongolian mare using distilled water, phosphate buffer saline and Tris-HCl, by use of 4 methods of protein extraction and precipitation with distilled water, sulfate ammonium salt and organic solvents such as ethanol an

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13

Morsy, Shaimaa. "The Effectiveness of Placental Extract Gel in the Treatment of Recurrent Aphthous Stomatitis: Randomized Clinical." Egyptian Dental Journal 68, no. 3 (2022): 2255–63. http://dx.doi.org/10.21608/edj.2022.127998.2029.

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14

LIU, GUOHUI, XI CHEN, WU ZHOU, et al. "Preparation of a novel composite nanofiber gel-encapsulated human placental extract through layer-by-layer self-assembly." Experimental and Therapeutic Medicine 11, no. 4 (2016): 1447–52. http://dx.doi.org/10.3892/etm.2016.3084.

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15

PITSON, Stuart M., Richard J. D'ANDREA, Lucianne VANDELEUR, et al. "Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes." Biochemical Journal 350, no. 2 (2000): 429–41. http://dx.doi.org/10.1042/bj3500429.

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Sphingosine 1-phosphate (S1P) is a novel lipid messenger that has important roles in a wide variety of mammalian cellular processes including growth, differentiation and death. Basal levels of S1P in mammalian cells are generally low, but can increase rapidly and transiently when cells are exposed to mitogenic agents and other stimuli. This increase is largely due to increased activity of sphingosine kinase (SK), the enzyme that catalyses its formation. In the current study we have purified, cloned and characterized the first human SK to obtain a better understanding of its biochemical activit

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16

Lacroix, MC, H. Jammes, and G. Kann. "Occurrence of a growth hormone-releasing hormone-like messenger ribonucleic acid and immunoreactive peptide in the sheep placenta." Reproduction, Fertility and Development 8, no. 3 (1996): 449. http://dx.doi.org/10.1071/rd9960449.

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Growth hormone releasing factor (GHRH) has been described in the rat, mouse and human placentae. This study reports the presence of an immunoreactive GHRH activity (IR-GHRH) in the ovine placenta. This activity was detected by radioimmunoassay from day 50 (D50) until the end of pregnancy. Higher IR-GHRH concentration in placental tissue was observed on days 100 (543 +/- 123 pg/g) and 140 (550 +/- 62 pg/g) and, when compared with the GHRH content of the ovine hypothalamus (1.2 ng/hypothalamus), represents a considerable amount of GHRH per placenta (a mean of 200 ng). Perifused placenta explants

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17

Malik, A. S., and M. G. Low. "Conversion of human placental alkaline phosphatase from a high Mr form to a low Mr form during butanol extraction. An investigation of the role of endogenous phosphoinositide-specific phospholipases." Biochemical Journal 240, no. 2 (1986): 519–27. http://dx.doi.org/10.1042/bj2400519.

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Alkaline phosphatase in a wide range of tissues has been shown to be anchored in the membrane by a specific interaction with the polar head group of phosphatidylinositol. It has previously been suggested that the production of low Mr alkaline phosphatase during the commonly used butanol extraction procedure may result from the activation of an endogenous phosphoinositide-specific phospholipase C which removes the 1,2-diacylglycerol responsible for membrane anchoring. This conversion process was investigated in greater detail with human placenta used as the source of alkaline phosphatase. Mr an

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18

Thakur, Gagan, Shaji Thomas, Darpan Bhargava, and Ankit Pandey. "Does Topical Application of Placental Extract Gel on Postoperative Fibrotomy Wound Improve Mouth Opening and Wound Healing in Patients With Oral Submucous Fibrosis?" Journal of Oral and Maxillofacial Surgery 73, no. 7 (2015): 1439.e1–1439.e10. http://dx.doi.org/10.1016/j.joms.2015.03.020.

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19

Shen, Liu-Hong, Lei Fan, Yue Zhang, et al. "Antioxidant Capacity and Protective Effect of Cow Placenta Extract on D-Galactose-Induced Skin Aging in Mice." Nutrients 14, no. 21 (2022): 4659. http://dx.doi.org/10.3390/nu14214659.

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Placental extract has been used for skin care and delaying skin aging. Cow placenta is an abundant resource with a large mass, which has not been harnessed effectively. Cow placenta extract (CPE) has the functions of antioxidation, anti-inflammatory, promoting growth and development, and promoting hair growth. However, little is known about the effect of oral administration of cow placenta extract on skin conditions. Therefore, the present study aimed to investigate the antioxidant capacity of CPE in vitro and in vivo and its protective effect on d-galactose (D-gal) induced skin aging in mice.

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20

Margolis, Ben, Isinsu Kuzu, Marille Herrmann, Michele D. Raible, Eric Hsi, and Serhan Alkan. "Rapid Polymerase Chain Reaction–Based Confirmation of Cat Scratch Disease and Bartonella henselae Infection." Archives of Pathology & Laboratory Medicine 127, no. 6 (2003): 706–10. http://dx.doi.org/10.5858/2003-127-706-rpcrco.

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Abstract Context.—Cat scratch disease (CSD) commonly occurs secondary to Bartonella henselae infection, and the diagnosis has traditionally been made by microscopic findings, the identification of organisms by cytochemistry, and clinical history. However, cytochemical analysis tends to be very difficult to interpret, and histology alone may be insufficient to establish a definitive diagnosis of CSD. Objective.—To demonstrate the presence of B henselae in tissue suspected of involvement by CSD, using a novel polymerase chain reaction (PCR) assay. Design.—Isolates of B henselae (American Tissue

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21

Waldo, G. L., T. Evans, E. D. Fraser, J. K. Northup, M. W. Martin, and T. K. Harden. "Identification and purification from bovine brain of a guanine-nucleotide-binding protein distinct from Gs, Gi and Go." Biochemical Journal 246, no. 2 (1987): 431–39. http://dx.doi.org/10.1042/bj2460431.

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A guanine-nucleotide-binding protein (G-protein) was purified from cholate extracts of bovine brain membranes by sequential DEAE-Sephacel, Ultrogel AcA-34, heptylamine-Sepharose and Sephadex G-150 chromatography. Guanosine 5′-[gamma-[35S]thio]triphosphate (GTP[35S])-binding activity copurified with a 25,000 Da peptide and a 35,000-36,000 Da protein doublet. Neither pertussis toxin nor cholera toxin catalysed the ADP-ribosylation of a protein associated with the GTP[35S]-binding activity. Photoaffinity labelling of the purified protein with 8-azido[gamma-32P]GTP indicated that the GTP-binding s

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22

Nelson, J. L., and A. P. Kulkarni. "Partial purification and characterization of a peroxidase activity from human placenta." Biochemical Journal 268, no. 3 (1990): 739–43. http://dx.doi.org/10.1042/bj2680739.

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Peroxidases can metabolize a variety of xenobiotics to reactive intermediates capable of binding to protein or DNA. The potential role of these enzymes in fetotoxicity has not been explored. In this study, the presence of peroxidase activity was observed in human term and pre-term placenta. Human term placental peroxidase activity (HTPP) was partially purified by concanavalin A affinity chromatography from CaCl2 extracts of the particulate fraction. HTPP appears to be a membrane-bound glycoprotein. Arachidonic acid-dependent oxidation of guaiacol was not observed, suggesting that the peroxidas

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Martin‐Thouvenin, V., S. Uhlrich, JL Tayot, and M. Lanotte. "Towards large‐scale purification of natural CSF‐1 from human placenta tissue extracts." Biotechnology and Applied Biochemistry 12, no. 2 (1990): 176–87. http://dx.doi.org/10.1111/j.1470-8744.1990.tb00090.x.

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The monocyte‐macrophage colony‐stimulating factor (colony‐stimulating factor 1) is characterized and partially purified from industrially processed human tissues for the first time. A five‐step purification procedure using placenta tissue extracts furnished a 13,620‐fold enrichment of biological activity. This procedure includes a “pilot” scale anion‐exchange chromatography at pH 4.5, gel permeation, and lectin affinity separation followed by HPLC steps (hydrophobic interaction and C18 reverse‐phase chromatographies). The purified bioactive material, which stimulates only monocyte‐macrophage p

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Xiao, Q., X. Han, E. Arany, D. Hill, and TJ McDonald. "Human placenta and fetal membranes contain peptide YY1-36 and peptide YY3-36." Journal of Endocrinology 156, no. 3 (1998): 485–92. http://dx.doi.org/10.1677/joe.0.1560485.

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Extracts of human term amniotic, placental, and chorion/decidua tissue contained, respectively, 4.36 +/- 2.79 (pmol/g wet wt; mean +/- S.E.M.: n = 5). 2.78 +/- 0.5 (n = 5) and 0.68 +/- 0.68 (n = 5) peptide YY (PYY)-like immunoreactivity. Using a specific PYY antiserum, gel filtration chromatography and reverse-phase high performance liquid chromatography (HLPC), amniotic, placental and fetal intestinal tissue extracts were demonstrated to contain PYY-like immunoreactivity consisting of equal amounts of PYY1-36 and PYY3-36. The presence of pancreatic polypeptide was not detected in any of the e

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Zebua, Nilsya, Muharni Saputri, Winda Giovana Sijabat, et al. "Incision Wound Healing Test of Ethanolic Extract Gel from Salaon (Parsonsia alboflavescens [Dennst.] Mabb.) Leaves in Male Rats." Open Access Macedonian Journal of Medical Sciences 9, A (2021): 776–81. http://dx.doi.org/10.3889/oamjms.2021.6662.

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BACKGROUND: An incision wound is a wound caused by a sharp object. One of the plants that can be used as a traditional medicine for an incision wound is salaon leaves. The prepared form chosen is the gel because it is easy to use and its distribution is faster on the skin. AIM: The aim of the study was to explore whether salaon leaf extract gel meets the quality evaluation requirements and to know the effectiveness of ethanol extract gel of salaon (Parsonsia alboflavescens [Dennst] Mabb.) leaves to cure a scar on male rats. METHODS: Experimental method with salaon leaves as sample. Simplicia o

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Sanguanboonyaphong, Phitjira, Phaijit Sritananuwat, Sureewan Duangjit, et al. "Novel Synergistic Approach for Bioactive Macromolecules: Evaluating the Efficacy of Goat Placenta Extract in PEGylated Liposomes and Microspicules for Chemotherapy-Induced Hair Loss." Pharmaceuticals 17, no. 8 (2024): 1084. http://dx.doi.org/10.3390/ph17081084.

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Chemotherapy-induced hair loss is a distressing side effect of cancer treatment, and medical interventions are often needed to address this problem. The objectives of this study were to evaluate the bioactivity of goat placenta (GP) extract on both normal and chemotherapy-induced hair cells and to develop PEGylated liposomes (PL) and microspicule (MS) formulations for promoting hair growth in patients with chemotherapy-induced hair loss. The bioactivities of GP extract on human follicle dermal papilla (HFDP) cells and cells damaged by chemotherapy were assessed. GP extract was incorporated int

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Repin, N. V., L. N. Marchenko, Yu A. Chizh, T. P. Govorukha, and V. I. Strona. "Quantitative and Qualitative Composition of Rat Placental Cryoextracts prior to and after Lyophilization." Ukraïnsʹkij žurnal medicini, bìologìï ta sportu 7, no. 5 (2022): 278–82. http://dx.doi.org/10.26693/jmbs07.05.278.

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The purpose of the study was to investigate a peptide composition of cryoextracts from rat placental tissue using a gel-penetrating chromatography prior to and after low temperature storage and lyophilization. Materials and methods. Cryoextracts were harvested from tissue homogenates of the rat placenta with a method of freezing (-20oC, 1 day), subsequent thawing and centrifugation. The following types of cryoextracts were analyzed: cryoextracts-1 – immediately after preparation; cryoextracts-2 – frozen down to -196oC and stored for 1 month at this temperature; cryoextracts-3 – subjected to cr

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Nardid, O., S. Repina, E. Bobrova, Yu Govorova, S. Narozhnyi, and E. Rozanova. "Beneficial impact of human placenta extracts on erythrocyte membrane thermostability." Trakia Journal of Sciences 16, no. 3 (2018): 204–11. http://dx.doi.org/10.15547/tjs.2018.03.006.

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PURPOSE: To study the influence of human placenta extract (HPE) and its individual fractions on the thermal stability of human erythrocyte membrane. METHODS: HPE fractions were isolated by gel chromatography. Thermal hemolysis of erythrocytes, exposed to 55°C was measured spectrophotometrically. Cytosol microvscosity and barrier function of erythrocyte membranes at hyperthermia were investigated by EPR spin probe TEMPON. Thermal denaturation of erythrocyte membrane proteins were studied by differential scanning calorimetry. RESULTS: Pre-treatment of erythrocytes with HPE or its fractions inhib

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Panchakshari, Madhuri K., Nilesh J. Patel, Nimesh B. Thakkar, and Kenil Kirit Patel. "Pathogenesis, diagnosis and management of diabetic foot ulcers: a systematic review." International Journal of Research in Medical Sciences 10, no. 11 (2022): 2717. http://dx.doi.org/10.18203/2320-6012.ijrms20222890.

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Diabetic foot ulcers lead to substantial morbidity and impair quality of life with high treatment costs and enormous economic losses. Diabetic foot ulcers readily become chronic; all too often these wounds do not heal primarily. Treatment of chronic wounds should be essentially directed against the main etiologic factors responsible for the wound. There are different treatment approaches for wound healing in diabetic foot ulcers. If treatment is based on the pathological cause, it may give better results and it must be cost effective too. Hydrogel dressing, platelet rich plasma, placenta extra

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Berryman, M., R. Gary, and A. Bretscher. "Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis." Journal of Cell Biology 131, no. 5 (1995): 1231–42. http://dx.doi.org/10.1083/jcb.131.5.1231.

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Ezrin is a component of the microvillus cytoskeleton of a variety of polarized epithelial cells and is believed to function as a membrane-cytoskeletal linker. In this study, we isolated microvilli from human placental syncytiotrophoblast as a model system for biochemical analysis of ezrin function. In contrast to intestinal microvilli, ezrin is a major protein component of placental microvilli, comprising approximately 5% of the total protein mass and present at about one quarter of the molar abundance of actin. Gel filtration and chemical cross-linking studies demonstrated that ezrin exists m

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Setiatin, E. T., D. Sajuthi, B. Purwantara, and C. Talib. "Extraction and isolation of Ovine Pregnancy-Associated Glycoprotein (ovPAG) from cotyledon placenta of Garut sheep." Jurnal Ilmu Ternak dan Veteriner 14, no. 3 (2013): 208–15. https://doi.org/10.14334/jitv.v14i3.342.

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Pregnancy-associated glycoprotein (PAG) structurally related to aspartic protease, expressed in the outer epithelial cell layer (trophectoderm) of ungulate placenta. Ovine PAG (ovPAG) synthesized by mono- and binucleic trophoblast before complete implantation at Day 14-15. Of this, ovPAG could be used as a marker for early pregnancy. The objective of study was to extract and isolate PAG from placenta of Garut Sheep collected at term and to characterize their molecular weight. The procedures included extraction of protein at neutral pH (cotyledon was thawed, minced, added PBS, blended and centr

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Laksmitawati, Dian Ratih, Umi Marwati, Wahyu Widowati, Rachmawati Noverina, Tri Suciati, and Ahmad Faried. "Combined Treatment Effect of Topical CM-hWJMSCs and Oral Moringa oleifera extract on Wound Healing of Diabetic Rat." Open Access Macedonian Journal of Medical Sciences 11, A (2023): 23–30. http://dx.doi.org/10.3889/oamjms.2023.7551.

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AIMS: The aim of the study was to determine the activity of topical conditioned medium (CM) mesenchymal stem cells as single or combined with oral therapy of Moringa oleifera extract (ME) in lowering blood glucose levels and healing diabetic wounds. MATERIALS AND METHODS: Male Sprague-Dawley rats were induced hyperglycemia with Streptozotocin and injured by a biopsy punch on the dorsal side. Then, the rats were divided into five groups, namely, the CM group, which was given topically; the CM–ME group, which was given CM gel topically and ME orally; the CM–Met group, which was given Metformin (

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Sivaprasadarao, A., M. Boudjelal, and J. B. C. Findlay. "Solubilization and purification of the retinol-binding protein receptor from human placental membranes." Biochemical Journal 302, no. 1 (1994): 245–51. http://dx.doi.org/10.1042/bj3020245.

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The membrane receptor for retinol-binding protein (RBP) has been solubilized from human placental brush-border membranes with octyl-beta-glucoside, Nonidet P-40 and CHAPS. A method, based on the preferential precipitation of 125I-RBP-receptor complex with poly(ethylene glycol) 8000, was developed in order to measure the RBP-binding activity in the detergent extracts. The receptor was fairly stable (4 degrees C, 7 days) in octyl-beta-glucoside and Nonidet P-40, but quickly lost activity in CHAPS. The detergent-solubilized form retained all the properties characteristic of the membrane-bound pro

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Evans, L. W., S. Muttukrishna, P. G. Knight, and N. P. Groome. "Development, validation and application of a two-site enzyme-linked immunosorbent assay for activin-AB." Journal of Endocrinology 153, no. 2 (1997): 221–30. http://dx.doi.org/10.1677/joe.0.1530221.

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Abstract Monoclonal antibodies, specific for the βa and βb subunits of activin, were used to develop a new two-site ELISA for activin-AB. The assay had a detection limit of 0·19 ng/ml. High concentrations of activin-AB were found in bovine, ovine and porcine follicular fluids (FF), with less in human FF (1310, 1730, 688 and 7 ng/ml respectively). Recovery of spiked activin-AB standard from human, bovine and ovine FFs and from homogenized human placental extracts averaged 91%, 115%, 115% and 94% respectively. Within-plate coefficients of variation for different concentrations of activin-AB were

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Rangsimawong, Worranan, Sureewan Duangjit, Phaijit Sritananuwat, Tanasait Ngawhirunpat, and Praneet Opanasopit. "Potential of Deer Placenta Extract in Hair Cell Regeneration and Its Nanoniosome-Microspicule Gel as a Transfollicular Delivery System." Cosmetics 11, no. 6 (2024): 204. http://dx.doi.org/10.3390/cosmetics11060204.

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Background: Deer placenta extract (DPE), rich in bioactive macromolecules, promotes regenerative effects in both normal and damaged cells. However, effective delivery of these macromolecules through the skin remains a challenge. Objectives: To investigate the potential of DPE in regenerating hair cells and to develop a nanoniosome (NS) and microspicule (MS) formulation as a promising transfollicular delivery system. Methods: The bioactivity of DPE was assessed in human follicle dermal papilla (HFDP) cells, including cells damaged by chemotherapy. The NS-MS formulation was designed to deliver b

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36

Kajander, E. O., L. I. Kauppinen, R. L. Pajula, K. Karkola, and T. O. Eloranta. "Purification and partial characterization of human polyamine synthases." Biochemical Journal 259, no. 3 (1989): 879–86. http://dx.doi.org/10.1042/bj2590879.

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Spermidine synthase was purified to apparent homogeneity from human spleens (8700-fold) by affinity chromatography. The native enzyme was composed of two subunits of identical Mr (35,000) and showed an apparent Mr of 62,000 in pore-gradient gel electrophoresis. Its pI was 5.1, Spermine synthase was purified to apparent homogeneity from placenta (5300-fold) and from kidney (4600-fold). The native enzyme was composed of two subunits of identical Mr (45,000) and showed an apparent Mr of 78,000 in pore-gradient gel electrophoresis. In isoelectric focusing it revealed two bands, with pI values of 4

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37

Ivanov, L., O. Shcherbak, L. Derymedvid, V. Kravchenko, and O. Bezugla. "Study of the molecular mass fractions distribution of porcine placenta aqueous extract by the method of highly efficient gel permeation chromatography." Ukrainian biopharmaceutical journal, no. 1(62) (March 2, 2020): 18–24. http://dx.doi.org/10.24959/ubphj.20.252.

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38

Drabikowska, Alicja K., Lidia Halec, and David Shugar. "Purification and Properties of Adenosine Kinase from Rat Liver: Separation from Deoxyadenosine Kinase Activity." Zeitschrift für Naturforschung C 40, no. 1-2 (1985): 34–41. http://dx.doi.org/10.1515/znc-1985-1-209.

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Ion exchange and affinity chromatography techniques, similar to those previously reported for purification of adenosine kinase from human placenta, were applied to purification of rat liver adenosine kinase. The enzyme, purified 400-fold in 41% yield, was homogeneous on SDS- polyacrylamide gel electrophoresis, with a molecular weight of 52000. It specific activity, 18 μmol/min/mg protein, is the highest hitherto reported for this enzyme from mammalian sources. Chromatography on DEAE-cellulose removed about 98% of the phosphorylating activity towards 2′-deoxyadenosine present in the initial pH-

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39

Conforti, G., A. Zanetti, S. Colella, et al. "Interaction of fibronectin with cultured human endothelial cells: characterization of the specific receptor." Blood 73, no. 6 (1989): 1576–85. http://dx.doi.org/10.1182/blood.v73.6.1576.1576.

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Abstract In this study we provide a characterization of the fibronectin (FN) binding to endothelial cells (EC), and we identify the FN binding site on these cells. 125I-FN binding to EC in suspension was time dependent and reached a plateau at 4 h. Cold FN inhibited this interaction in a concentration-dependent way, but vitronectin, fibrinogen, and IgG were poorly effective. About 80% of the total FN associated to EC at the equilibrium was specifically bound; of this, 60% was reversibly bound, while 20% appeared to be internalized. The FN binding was saturable and an apparent dissociation cons

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Conforti, G., A. Zanetti, S. Colella, et al. "Interaction of fibronectin with cultured human endothelial cells: characterization of the specific receptor." Blood 73, no. 6 (1989): 1576–85. http://dx.doi.org/10.1182/blood.v73.6.1576.bloodjournal7361576.

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In this study we provide a characterization of the fibronectin (FN) binding to endothelial cells (EC), and we identify the FN binding site on these cells. 125I-FN binding to EC in suspension was time dependent and reached a plateau at 4 h. Cold FN inhibited this interaction in a concentration-dependent way, but vitronectin, fibrinogen, and IgG were poorly effective. About 80% of the total FN associated to EC at the equilibrium was specifically bound; of this, 60% was reversibly bound, while 20% appeared to be internalized. The FN binding was saturable and an apparent dissociation constant of a

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41

Cunnah, D., D. S. Jessop, G. M. Besser, and L. H. Rees. "Measurement of circulating corticotrophin-releasing factor in man." Journal of Endocrinology 113, no. 1 (1987): 123–31. http://dx.doi.org/10.1677/joe.0.1130123.

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ABSTRACT A radioimmunoassay was developed to measure corticotrophin-releasing factor (CRF-41) extracted from human plasma using Vycor glass. Assay sensitivity was 20 ng/l and intra- and interassay coefficients of variation were 10·2 and 11·4% respectively. The normal range of plasma CRF-41 was <20–110ng/l (n = 46). Plasma concentrations of CRF-41 in patients with Cushing's disease, Nelson's syndrome and Addison's disease were within the normal range. No correlation was found between CRF-41 and ACTH in these syndromes. Two patients with the ectopic ACTH syndrome had increased plasma concentr

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42

Repin, Mykola, Yuliia Chizh, Larysa Marchenko, Tetyana Govorukha, and Stanislav Narozhnyy. "Composition and Biological Activity of Fetoplacental Tissues-Derived Cryoextracts Being Differently Obtained." Problems of Cryobiology and Cryomedicine 33, no. 1 (2023): 3–13. http://dx.doi.org/10.15407/cryo33.01.003.

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The protein-peptide composition of fetal tissues (FTCEs) and placenta (PCE) cryoextracts of rats was investigated by gel permeation chromatography. Cryoextracts were derived from tissue homogenates using the freeze-warming modes: 1 – single (–20°С); 2 – double (–20; –196°С) and 3 – triple (–20; –196; –196°С) ones. The biological activity of cryoextracts was in vitro evaluated by the phagocytic activity of neutrophil granulocytes (NG) of the blood of intact rats after incubation with an inactivated culture of Staphylococcus aureus (2 × 109 cells/ml) for 45 and 120 min for the concentrations of

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43

Soos, M. A., and K. Siddle. "Immunological relationships between receptors for insulin and insulin-like growth factor I. Evidence for structural heterogeneity of insulin-like growth factor I receptors involving hybrids with insulin receptors." Biochemical Journal 263, no. 2 (1989): 553–63. http://dx.doi.org/10.1042/bj2630553.

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The receptors for insulin and insulin-like growth factor-I (IGF-I) are closely related in primary sequence and overall structure. We have examined the immunological relationships between these receptors by testing the reactivity of anti-(insulin receptor) monoclonal antibodies with IGF-I receptors in various tissues and cell lines. Antibodies for six distinct epitopes reacted with a subfraction of IGF-I receptors, as shown by inhibition of 125I-IGF-I binding, precipitation of 125I-IGF-I-receptor complexes or immunodepletion of receptor from tissue extracts before binding assays. Both immunorea

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44

Talbot, N., A. Powell, T. Caperna, and W. Garrett. "73 PROTEOMIC ANALYSIS OF THE MAJOR CELLULAR PROTEINS OF BOVINE TROPHECTODERM CELL LINES DERIVED FROM IVP, PARTHENOGENETIC, AND NUCLEAR TRANSFER EMBRYOS: REDUCED EXPRESSION OF ANNEXIN I IN NUCLEAR TRANSFER-DERIVED CELL LINES." Reproduction, Fertility and Development 18, no. 2 (2006): 145. http://dx.doi.org/10.1071/rdv18n2ab73.

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Trophectoderm cell lines were established from 8-day in vitro cultured bovine embryos that were derived from the fertilization (IVF), somatic cell nuclear transfer (NT), or parthenogenetic activation (P) of in vitro-matured oocytes and from five 8-day-old in vivo (V) embryos. The most abundant cellular proteins of 2 V-, 16 NT-, 12 P-, and 13 IVF-derived cell lines were compared by 2-D gel electrophoresis and mass spectrometry; that is, the unaltered thiourea/urea extract of each cell culture was analyzed. One-hundred and eighteen in common protein spots were examined, and 95% were identified w

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Bahouth, S. W., та C. C. Malbon. "Human β-adrenergic receptors. Simultaneous purification of β1- and β2-adrenergic-receptor peptides". Biochemical Journal 248, № 2 (1987): 557–66. http://dx.doi.org/10.1042/bj2480557.

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Beta-Adrenergic receptors from basal membranes of human placenta were purified from digitonin extracts by sequential rounds of affinity chromatography, hydrophobic chromatography, ion-exchange chromatography and steric-exclusion h.p.l.c. Basal membranes display both beta 1- and beta 2-adrenergic receptors, in the ratio 65:35. Affinity chromatography, hydrophobic chromatography on heptylamine-Sepharose and ion-exchange chromatography on DEAE-Sephacel removed most of the contaminating proteins, and final purification of the receptor to apparent homogeneity was achieved by steric-exclusion h.p.l.

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46

Reddy, SV, O. Alcantara, GD Roodman, and DH Boldt. "Inhibition of tartrate-resistant acid phosphatase gene expression by hemin and protoporphyrin IX. Identification of a hemin-responsive inhibitor of transcription." Blood 88, no. 6 (1996): 2288–97. http://dx.doi.org/10.1182/blood.v88.6.2288.bloodjournal8862288.

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Tartrate-resistant acid phosphatase (TRAP) is an iron-containing protein encoded by the same gene that codes for uteroferrin, a placental iron transport protein. In human peripheral mononuclear cells, TRAP expression is inhibited by both hemin (ferric protoporphyrin IX) and protoporphyrin IX. Nuclear run-on assays confirmed that this inhibition occurs at the level of gene transcription. Previous studies with mTRAP deletion mutants showed that the hemin effect was dependent on repressor activity in the mTRAP 5′- flanking region at -1846 bp to -1240 bp relative to ATG (Reddy et al, J Bone Minera

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47

Robinson, M. S., and B. M. Pearse. "Immunofluorescent localization of 100K coated vesicle proteins." Journal of Cell Biology 102, no. 1 (1986): 48–54. http://dx.doi.org/10.1083/jcb.102.1.48.

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A family of coated vesicle proteins, with molecular weights of approximately 100,000 and designated 100K, has been implicated in both coat assembly and the attachment of clathrin to the vesicle membrane. These proteins were purified from extracts of bovine brain coated vesicles by gel filtration, hydroxylapatite chromatography, and preparative SDS PAGE. Peptide mapping by limited proteolysis indicated that the polypeptides making up the three major 100K bands have distinct amino acid sequences. When four rats were immunized with total 100K protein, each rat responded differently to the differe

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48

Yelo, Estefania, Lourdes Gimeno, Maria Victoria Bernardo, Maria Juliana Majado, Maria Rocio Alvarez, and Antonio Parrado. "Molecular Cloning of DOCK10/Zizimin3, a Novel Cdc42/Rac-Interacting Protein Specifically Induced by IL4 in B Lymphocytes." Blood 108, no. 11 (2006): 939. http://dx.doi.org/10.1182/blood.v108.11.939.939.

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Abstract Interleukin-4 (IL4) induces proliferation, differentiation and survival of B lymphocytes. IL4 protects CLL B cells from death by apoptosis. Gene expression analysis suggest that IL4 pathways are activated in CLL cells. We have identified DOCK10/Zizimin3 as an IL4-induced gene in CLL cells, and have obtained its full length sequence after cloning 1960 bp at its 5′ terminus by RACE-PCR. The human DOCK10/ZIZ3 sequence coded for a protein with 2180 amino acids and a predicted Mr of 250K. DOCK10/ZIZ3 shared homology with the other two members of the Zizimin family, and is the largest among

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49

Kerr, D. E., B. Laarveld, and J. G. Manns. "Effects of passive immunization of growing guinea-pigs with an insulin-like growth factor-I monoclonal antibody." Journal of Endocrinology 124, no. 3 (1990): 403–15. http://dx.doi.org/10.1677/joe.0.1240403.

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ABSTRACT The physiological importance of circulating as opposed to locally produced insulin-like growth factor-I (IGF-I) has not been determined. By using a passive immunoneutralization technique, our objectives were to evaluate the role of circulating IGF-I in the regulation of animal growth and pituitary GH content. A monoclonal antibody (MAb) to IGF-I, generated in our laboratory, has an affinity (Ka) of 0·13 litres/pmol for recombinant human IGF-I (rhIGF-I). Cross-reactivities of recombinant des-tripeptide IGF-I and recombinant bovine IGF-II were approximately 40 and 8% respectively. This

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50

Chowdhury, Uttam. "Regulation of transgelin and GST-pi proteins in the tissues of hamsters exposed to sodium arsenite." International Journal of Toxicology and Toxicity Assessment 1, no. 1 (2021): 1–8. http://dx.doi.org/10.55124/ijt.v1i1.49.

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Hamsters were exposed to sodium arsenite (173 mg As/L) in drinking water for 6 days. Equal amounts of proteins from urinary bladder or liver extracts of control and arsenic-treated hamsters were labeled with Cy3 and Cy5 dyes, respectively. After differential in gel electrophoresis and analysis by the DeCyder software, several protein spots were found to be down-regulated and several were up regulated. Our experiments indicated that in the bladder tissues of hamsters exposed to arsenite, transgelin was down-regulated and GST-pi was up-regulated. The loss of transgelin expression has been report

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