Relevant bibliographies by topics / Plant cell biotechnology

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers
  6. Reports

Academic literature on the topic 'Plant cell biotechnology'

Author: Grafiati
Published: 4 June 2021
Last updated: 30 January 2023

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Journal articles on the topic "Plant cell biotechnology"

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Schumacher, H. M. "Plant cell biotechnology." Plant Science 102, no. 1 (January 1994): 118–19. http://dx.doi.org/10.1016/0168-9452(94)90027-2.

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Stafford, A., P. Morris, and M. W. Fowler. "Plant cell biotechnology: A perspective." Enzyme and Microbial Technology 8, no. 10 (October 1986): 578–87. http://dx.doi.org/10.1016/0141-0229(86)90114-6.

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Chapple, Clint, and Nick Carpita. "Plant cell walls as targets for biotechnology." Current Opinion in Plant Biology 1, no. 2 (April 1998): 179–85. http://dx.doi.org/10.1016/s1369-5266(98)80022-8.

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Harborne, Jeffrey B. "Advances in plant cell biochemistry and biotechnology:." Phytochemistry 35, no. 1 (December 1993): 277. http://dx.doi.org/10.1016/s0031-9422(00)90553-3.

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Kostenyuk, I. A., O. F. Lubaretz, V. V. Voronin, and Yu Yu Gleba. "Cell engineering developed for apocynaceae plant biotechnology." Biopolymers and Cell 7, no. 4 (July 20, 1991): 26–34. http://dx.doi.org/10.7124/bc.0002dc.

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Alfermann, A. W., and Maike Petersen. "Natural product formation by plant cell biotechnology." Plant Cell, Tissue and Organ Culture 43, no. 2 (November 1995): 199–205. http://dx.doi.org/10.1007/bf00052176.

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Hamann, Thorsten, Anna Kärkönen, and Kirsten Krause. "From plant cell wall metabolism and plasticity to cell wall biotechnology." Physiologia Plantarum 164, no. 1 (August 22, 2018): 2–4. http://dx.doi.org/10.1111/ppl.12794.

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Nourani, Amira, Elena Popova, and Maria Titova. "Biotechnology based on cell cultures of higher plants." E3S Web of Conferences 265 (2021): 04012. http://dx.doi.org/10.1051/e3sconf/202126504012.

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This paper reviews the role of plant cell culture as a biotechnological tool in preserving the botanical diversity of higher plants while meeting the growing demand of the commercial market for large volumes of plant raw material. The prospects of plant cell-based technology are discussed in the framework of creating an economy of sustainable development in the short and long term.
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Zhang, Baohong, and Qinglian Wang. "MicroRNA-Based Biotechnology for Plant Improvement." Journal of Cellular Physiology 230, no. 1 (September 29, 2014): 1–15. http://dx.doi.org/10.1002/jcp.24685.

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Scragg, A. H. "Plant cell culture." Journal of Biotechnology 26, no. 1 (October 1992): vii. http://dx.doi.org/10.1016/0168-1656(92)90066-i.

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Dissertations / Theses on the topic "Plant cell biotechnology"

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Cheung, Caleb Kin Lok Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Effects of imperfect mixing in suspended plant and animal cell cultures." Awarded by:University of New South Wales. School of Biotechnology and Biomolecular Sciences, 2006. http://handle.unsw.edu.au/1959.4/25200.

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A common problem observed in large-scale cell cultivation is reduced culture performance compared with small-scale processes due to the existence of concentration gradients caused by poor mixing. Small-scale simulations using microbial cell suspensions have shown that circulation of cells through concentration gradients of oxygen, pH and glucose can result in reduction of cell growth and product formation similar to the effects observed in large-scale bioreactors. This study was aimed at using scale-down studies to investigate poor mixing in large-scale bioreactors used for suspended plant and animal cell culture. Two plant cell suspensions and a hybridoma cell line were used in this work. The range of oxygen transfer coefficients achieved in the hybridoma and plant suspensions were about 50???20 h-1 and 12???6 h-1, respectively. One-vessel simulation was developed to induce fluctuations of dissolved oxygen tension in a 2-L bioreactor using intermittent sparging of air and nitrogen. The effect of dissolved oxygen fluctuations on the cells was examined by comparing the performance of the cultures with those operated at constant dissolved oxygen tension. In the hybridoma suspension culture, only slight effects on cell growth were observed at circulation times above 300 s. No effect on the specific glucose uptake rate or antibody production was observed at the circulation times tested. Analysis of gene expression for selected hypoxia-related genes also suggested that the overall effect was limited. In plant cell suspensions, the specific growth rates and biomass yields on total sugar in the cultures under fluctuating dissolved oxygen tension were essentially the same as those at constant dissolved oxygen tension for both transgenic Nicotiana tabacum and Thalictrum minus. Under fluctuating dissolved oxygen tension, no effect on antibody accumulation was observed in transgenic N. tabacum suspensions, but a decrease in berberine accumulation was observed in T. minus. From the results, it can be concluded that only minimal effects due to the development of concentration gradients would be expected in large-scale bioreactors used for the cultivation of the hybridoma and plant cell suspensions tested in this work.
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Bekker, Jan P. I. "Genetic manipulation of the cell wall composition of sugarcane." Thesis, Link to online version, 2007. http://hdl.handle.net/10019/336.

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Archambault, Jean. "Surface immobilization of plant cells." Thesis, McGill University, 1987. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=28397.

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A novel technique was developed to immobilize plant cells. The cells are deposited on a surface of man-made fibrous material which provides for strong binding of the plant tissue biomass growing in the submerged culture. It was shown that the plant cells need to be fully viable for the attachment process to occur.
The scale-up of this technique to laboratory size specifically designed bioreactors was performed successfully. The cell immobilizing matrix was formed into a vertical spirally wound configuration to provide for a high immobilizing area-to-volume ratio (0.8-1.2 cm$ sp{-1}$). A modified airlift (riser-to-downcomer area ratio of 0.03 and vessel height-to-diameter (H/D ratio of 3) and a low H/D ($ sim$1.5) mechanically stirred vessel delivered the optimum bioreactor performance characterized by low foaming of the broth and highly efficient plant cell attachment and retention ($ geq$96%).
The growth of Catharantus roseus plant cells was investigated in these bioreactors. This process was found not to be mass transfer limited above minimal mild mixing and aeration levels ensuring sufficient supply of nutrients, especially oxygen (k$ sb{ rm L}$a $ sim$ 10-15 h$ sp{-1}$) to the immobilized biomass.
The gentle surface immobilization technique developed in this work did not hinder the biosynthesis potential of the SIPC. In fact, it appeared to induce a partial secretion of some valuable compounds into the culture medium. The mildness, easiness, efficiency, mass transfer characteristics, scale-up potential and biomass loading capacity (11-13 g d.w./L) of the surface immobilization technique make it superior to all other immobilization techniques used to culture plant cells. In addition, its bioreactor overall biomass concentration compares favourably to suspended plant cell concentrations attainable in bioreactors (15-20 g d.w./L).
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Yin, Zhao. "Characterization of the biological function of AtEXO70E2." HKBU Institutional Repository, 2018. https://repository.hkbu.edu.hk/etd_oa/483.

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Exocyst positive organelle (EXPO) is a newly discovered double membrane organelle involved in exocytosis and likely other vesicle trafficking processes. EXPO is likely generated from the ER, fused with plasma membrane and released a single membrane vesicle to cell exterior. The Arabidopsis protein Exo70E2 was found to be associated with EXPO and therefore is considered as a marker of EXPO and might play a role in EXPO-mediated vesicle trafficking. Understanding the biological function of AtExo70E2 (abbreviated as E2 in this thesis) will be very helpful in unraveling the function of EXPO. The aim of this work was to use various molecular, genetic and physiological approaches to determine the possible role of Arabidopsis Exo70E2 in biological pathways. By using the Exo70E2pro:GUS line, the expression pattern of Exo70E2 was determined. Exo70E2 was expressed mainly in roots, especially in root tips and epidermal cells in the division and elongation zones of roots. Its expression level was induced when the seedlings were treated with Flg22, a peptide derived from bacterial flagillin protein that induces the plant defense response. The tissue subcellular localization of Exo70E2 was also studied using the 35S:Exo70E2-eYFP and Exo70E2pro:Exo70E2-GFP reporter lines. The GFP fusion protein was found primarily in the epidermal cells of roots even in the 35S:Exo70E2-eYFP lines. For phenotypic analysis resulting from mutations of the Exo70E2 gene, I obtained three T-DNA insertion mutant lines and generated its overexpression lines. The two mutant alleles, e2-2 and e2-3 are in the Columbia ecotype background and further characterized. e2-2 which has a T-DNA insertion in an exon is likely a knock out line as Exo70E2 gene transcript could not be detected. e2-3, which carries a T-DNA insertion in its promoter region, was found to accumulate a higher level of the transcript, suggesting that the insertion causes its enhanced expression of Exo70E2. There was no obvious difference between wild type and e2-2 in their phenotypes under different conditions tested in this study. However, e2-3 had a retarded growth phenotype when grown in soil or on MS medium. The seedlings of e2-3 on MS medium also had a yellowish color although such a phenotype was not obvious when they were grown in soil. When supplementing the MS medium with sucrose, glucose or mannitol, the growth of e2-3 was more reduced compared to wild type under these conditions. However, on the medium with NaCl or under phosphate deficiency, the yellowish phenotype of e2-3 was rescued and the mutant seedlings became relatively healthier than the seedlings under the regular MS medium. A proteomics approach was taken to compare protein secreted from the seedlings of wild type and the mutants. Proteins secreted by seedlings to the liquid medium were collected, concentrated and subjected to MS analysis. Comparison of the profiles of secreted proteins between the wild type and the mutants leaded to identification of candidate proteins whose secretion might be affected by the mutation. My study indicates that Exo70E2 and EXPO are involved in transporting proteins (likely also metabolites) to the exterior of cells and the rhizosphere and might play an important role in stress responses.
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Ayeleso, Taiwo Betty. "Protoplast isolation and plant regeneration in Bambara groundnut : a platform for transient gene expression." Thesis, Cape Peninisula University of Technology, 2016. http://hdl.handle.net/20.500.11838/2003.

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Thesis (MTech (Agriculture))--Cape Peninsula University Of Technology, 2016.
Bambara groundnut (Vigna subterranea), a dicotyledonous plant is a legume which has a potential to contribute to food security and nutrition. Protoplasts are naked plant cells lacking cell walls. Viable protoplasts are potentially totipotent. Therefore, when given the correct stimuli, each protoplast is capable, theoretically, of regenerating a new wall and undergoing repeated mitotic division to produce daughter cells from which fertile plants may be regenerated through the tissue culture process. Protoplast systems are valuable and versatile cell based systems that are useful in observing cellular processes and activities. In this study, the isolation of protoplast from the leaves of Bambara groundnut plant was extensively optimised. The factors affecting protoplast isolation considered in this study were ages of plant material, mannitol concentration, combinations and concentrations of enzymes and duration of incubation. Effects of ages of Bambara groundnut plant (4, 6, 8, 10 weeks), molarities of mannitol (0.4 M, 0.5 M. 0.6 M and 0.7 M), concentration and combination of enzymes (1%, 2% and 4% cellulase, 0.5% and 1% macerozyme and, 0.5% and 1% pectinase) at different incubation duration (4, 18, 24, 42 hours) were investigated. Overall, it can be deduced from this study that the optimal protoplast yield (4.6 ± 0.14×105ml-1/gFW) and viability (86.5 ± 2.12%) were achieved by digesting the leaves of four week old Bambara groundnut plant with 2% cellulase and 0.5 % macerozyme with 0.5M mannitol for 18 hours. Freshly isolated protoplasts were then cultured at different densities of 1 × 104 - 2 ×106 protoplasts/ml using MS in three different culture (Liquid, agar and agarose bead) methods. First cell division was observed only in liquid medium. With several attempts, no division was achieved in the agar and agarose bead methods, division also did not progress in the liquid medium and hence, plant regeneration from Bambara groundnut protoplasts could not be achieved in this study. Consequently, a further study is underway to compare the proteomic profiles of freshly isolated protoplasts and cultured protoplasts in order to gain insights into the expression of proteins that could perhaps be contributing to the difficulty in regenerating Bambara groundnut plant through protoplast technology. The present study is novel because it is the first study to optimise the various factors that could affect protoplast isolation from the leaves of Bambara groundnut and thus developed an efficient protocol for protoplasts isolation from leaves of Bambara groundnut for cell manipulation studies.
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Guerreiro, Catarina Isabel Proença Duarte. "Molecular mechanisms of plant cell wall hydrolysis: developing novel biotechnological applications for carbohydrate-binding modules." Doctoral thesis, Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária, 2008. http://hdl.handle.net/10400.5/227.

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Tese de Doutoramento em Ciências Veterinárias
Xyloglucan (XG) is the major plant cell wall hemicellulose. Biochemical and structural studies on a xyloglucanase, Xgh74A, from Clostridium thermocellum are presented here. In addition, the carbohydrate-binding modules (CBMs) from C. thermocellum Cel9D-Cel44A, CtCBM30 and CtCBM44, which recognize XG, are characterized in this work. CtCel9D-Cel44A’s C-terminal module of unknown function is a CBM, constituting the founder member of family 44. Structural studies revealed that both CtCBM30 and CtCBM44 present Type B binding site topologies where tryptophans play an important role in ligand recognition. Cel44A is an endoglucanase domain displaying some xylanase activity, which action is potentiated by CtCBM44 through a targeting effect. Biochemical and structural studies on CtLic26A-Cel5E are also described here. CtLic26A is a mixed b-1,4-b-1,3-glucanase and Cel5E an endo-b-1,4- glucanase. The three-dimensional structure of CtLic26A provides insights in the mechanism of lichenan recognition by family 26 glycoside hydrolases. Finally, novel biotechnological applications for CBMs were investigated. An experiment was conducted where a barley-based diet for broilers was supplemented with CtLic26ACel5 derivatives, with or without CtCBM11, and with a commercial enzyme. The fusion of four antimicrobial peptides with CipA from CtCBM3 originated recombinants with high affinity for crystalline cellulose, indicating CBMs could fix bioactive molecules to cellulose.
RESUMO: O xiloglucano é a principal hemicelulose das paredes celulares vegetais. Neste trabalho apresentam-se estudos bioquímicos e estruturais sobre a xiloglucanase Xgh74A do Clostridium thermocellum. São também estudados módulos de ligação a carbohidratos (CBMs) com afinidade para o xiloglucano, o CtCBM30 e o CtCBM44 da CtCel9DCel44A. O módulo C-terminal revelou-se um CBM, sendo o membro fundador da família 44. As estruturas cristalográficas desses CBMs mostram locais de ligação aos polissacáridos com topologia do Tipo B, nos quais os triptofanos representam um papel importante no reconhecimento dos ligandos. A Cel44A é uma endoglucanase com actividade xilanásica, cuja acção é potenciada pelo CtCBM44. Estudos bioquímicos e estruturais sobre a CtLic26A-Cel5E são também apresentados, sendo a CtLic26A uma b-1,4-b-1,3-glucanase e a Cel5E uma endo-b-1,4-glucanase. A estrutura tridimensional da CtLic26A revelou os mecanismos estruturais que modulam a especificidade da enzima. Neste trabalho são pesquisadas novas aplicações biotecnológicas para os CBMs. Efectuou-se um ensaio com frangos de carne, suplementando dietas à base de cevada com derivados recombinantes da CtLic26A-Cel5, com e sem CtCBM11, e com uma enzima comercial. A fusão de quatro péptidos antimicrobianos com a CipA do CtCBM3 originou recombinantes com elevada afinidade para celulose cristalina, indicando que os CBMs poderão fixar moléculas bioactivas a materiais celulósicos.
This work was funded by Fundação para a Ciência e a Tecnologia, grant SFRH/BD/16731/2004, and co-funded by POCI 2010 and FSE from Ministério da Ciência, Tecnologia e Ensino Superior
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Maschke, Rüdiger W., Katja Geipel, and Thomas Bley. "Modeling of plant in vitro cultures – overview and estimation of biotechnological processes." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2017. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-216328.

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Plant cell and tissue cultivations are of growing interest for the production of structurally complex and expensive plant-derived products, especially in pharmaceutical production. Problems with up-scaling, low yields and high-priced process conditions result in an increased demand for models to provide comprehension, simulation, and optimization of production processes. In the last 25 years, many models have evolved in plant biotechnology; the majority of them are specialized models for a few selected products or nutritional conditions. In this article we review, delineate, and discuss the concepts and characteristics of the most commonly used models. Therefore, the authors focus on models for plant suspension and submerged hairy root cultures. The article includes a short overview of modeling and mathematics and integrated parameters, as well as the application scope for each model. The review is meant to help researchers better understand and utilize the numerous models published for plant cultures, and to select the most suitable model for their purposes.
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Maschke, Rüdiger W., Katja Geipel, and Thomas Bley. "Modeling of plant in vitro cultures – overview and estimation of biotechnological processes." WILEY-VCH Verlag GmbH & Co. KGaA, 2015. https://tud.qucosa.de/id/qucosa%3A30073.

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Plant cell and tissue cultivations are of growing interest for the production of structurally complex and expensive plant-derived products, especially in pharmaceutical production. Problems with up-scaling, low yields and high-priced process conditions result in an increased demand for models to provide comprehension, simulation, and optimization of production processes. In the last 25 years, many models have evolved in plant biotechnology; the majority of them are specialized models for a few selected products or nutritional conditions. In this article we review, delineate, and discuss the concepts and characteristics of the most commonly used models. Therefore, the authors focus on models for plant suspension and submerged hairy root cultures. The article includes a short overview of modeling and mathematics and integrated parameters, as well as the application scope for each model. The review is meant to help researchers better understand and utilize the numerous models published for plant cultures, and to select the most suitable model for their purposes.
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Geipel, Katja, Maria Lisa Socher, Christiane Haas, Thomas Bley, and Juliane Steingroewer. "Growth kinetics of a Helianthus annuus and a Salvia fruticosa suspension cell line: Shake flask cultivations with online monitoring system." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-213256.

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Plants produce a variety of secondary metabolites, e.g. to defend themselves against herbivores or to attract pollinating insects. Plant cell biotechnology offers excellent opportunities in order to use such secondary plant metabolites to produce goods with consistent quality and quantity throughout the year, and therefore to act independently from biotic and abiotic environmental factors. This article presents results of an extensive study of plant cell in vitro cultivation in a modern shake flask system with non-invasive online respiration activity monitoring unit. Comprehensive screening experiments confirm the successful transfer of a model culture (sunflower suspension) into the shake flask monitoring device and the suitability of this respiration activity monitoring unit as qualified tool for screening of plant in vitro cultures (sunflower and sage suspension). The authors demonstrate deviations between online and offline data due to varying water evaporation from different culture flask types. The influence of evaporation on growth-specific parameters thereby rises with increasing cultivation time. Furthermore, possibilities to minimize the impact of evaporation, either by adjusting the inlet air moisture or by measuring the evaporation in combination with an appropriate correction of the measured growth values, are shown.
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Geipel, Katja, Maria Lisa Socher, Christiane Haas, Thomas Bley, and Juliane Steingroewer. "Growth kinetics of a Helianthus annuus and a Salvia fruticosa suspension cell line: Shake flask cultivations with online monitoring system." WILEY-VCH Verlag GmbH & Co. KGaA, 2013. https://tud.qucosa.de/id/qucosa%3A29933.

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Plants produce a variety of secondary metabolites, e.g. to defend themselves against herbivores or to attract pollinating insects. Plant cell biotechnology offers excellent opportunities in order to use such secondary plant metabolites to produce goods with consistent quality and quantity throughout the year, and therefore to act independently from biotic and abiotic environmental factors. This article presents results of an extensive study of plant cell in vitro cultivation in a modern shake flask system with non-invasive online respiration activity monitoring unit. Comprehensive screening experiments confirm the successful transfer of a model culture (sunflower suspension) into the shake flask monitoring device and the suitability of this respiration activity monitoring unit as qualified tool for screening of plant in vitro cultures (sunflower and sage suspension). The authors demonstrate deviations between online and offline data due to varying water evaporation from different culture flask types. The influence of evaporation on growth-specific parameters thereby rises with increasing cultivation time. Furthermore, possibilities to minimize the impact of evaporation, either by adjusting the inlet air moisture or by measuring the evaporation in combination with an appropriate correction of the measured growth values, are shown.
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Books on the topic "Plant cell biotechnology"

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Endress, R. Plant cell biotechnology. Berlin: Springer-Verlag, 1994.

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NATO Advanced Study Institute on Plant Cell Biotechnology (1987 Albufeira, Portugal). Plant cell biotechnology. Berlin: Springer-Verlag, 1988.

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Endress, Rudolf. Plant Cell Biotechnology. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-02996-1.

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Pais, M. Salomé S., F. Mavituna, and J. M. Novais, eds. Plant Cell Biotechnology. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73157-0.

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Zhong, J. J., and S. Y. Byun. Plant cells. Berlin: Springer, 2010.

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Cell-based biosensors. Lancaster, Pa: Technomic Pub. Co., 1995.

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R, Spier, ed. Encyclopedia of cell technology. New York: Wiley-Interscience, 2000.

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NATO, Advanced Research Workshop on Plant Vacuoles: Their Importance in Plant Cell Compartmentation and Their Applications in Biotechnology (1986 Sophia-Antipolis France). Plant vacuoles: Their importance in solute compartmentation in cells and their applications in plant biotechnology. New York: Plenum Press, 1987.

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NATO Advanced Research Workshop on Plant Vacuoles (1986 Sophia-Antipolis, France). Plant vacuoles: Their importance in solute compartmentation in cells and their applications in plant biotechnology. New York: Plenum in cooperation with NATO Scientific Af fairs Division, 1987.

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Cleere, Michael F. The effects of process conditions on plant cell viability. Dublin: University College Dublin, 1995.

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Book chapters on the topic "Plant cell biotechnology"

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Petersen, Maike, and August Wilhelm Alfermann. "Plant Cell Cultures." In Biotechnology, 577–614. Weinheim, Germany: Wiley-VCH Verlag GmbH, 2008. http://dx.doi.org/10.1002/9783527620821.ch17.

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Fowler, Michael W., and Alan H. Scragg. "Natural Products from Higher Plants and Plant Cell Culture." In Plant Cell Biotechnology, 165–77. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73157-0_17.

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Mavituna, F. "Introduction to Plant Biotechnology." In Plant Cell Biotechnology, 1–14. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73157-0_1.

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Endress, Rudolf. "Plant Regeneration: Morphogenesis." In Plant Cell Biotechnology, 99–120. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-02996-1_5.

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Magnien, E. "Plant Biotechnology and Community Development." In Plant Cell Biotechnology, 483–95. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73157-0_47.

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Staba, E. John. "Future Trends in Plant Cell Biotechnology." In Plant Cell Biotechnology, 445–59. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73157-0_44.

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Yoshioka, Toshihiro, and Yasuhiro Fujita. "Economic Aspects of Plant Cell Biotechnology." In Plant Cell Biotechnology, 475–82. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73157-0_46.

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Endress, Rudolf. "Culturing of Plant Cells." In Plant Cell Biotechnology, 46–88. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-02996-1_3.

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Endress, Rudolf. "Immobilization of Plant Cells." In Plant Cell Biotechnology, 256–69. Berlin, Heidelberg: Springer Berlin Heidelberg, 1994. http://dx.doi.org/10.1007/978-3-662-02996-1_7.

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Reisch, Bruce I. "Genetic Instability in Plant Cell Cultures: Utilization in Plant Breeding and Genetic Studies." In Plant Cell Biotechnology, 87–95. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-73157-0_10.

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Conference papers on the topic "Plant cell biotechnology"

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"The instrumental cultivation of Phlojodicarpus sibiricus cell culture." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-092.

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"Plant cell wall as a target for functional genomics." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-068.

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"The genetic variability of proliferative cell lines of Larix sibirica." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-197.

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"Creation of early maturing productive forms of cereals using the cell biotechnology and physiologically active compounds." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 2019. http://dx.doi.org/10.18699/plantgen2019-028.

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Khandy, M. T., S. V. Tomilova, D. V. Kochkin, and A. M. Nosov. "Initiation and characterization of plant cell culture of Phlojodicarpus sibiricus." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.250.

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In the framework of the present study, callus and suspension cultures of Phlojodicarpus sibiricus cells were obtained. Their morphological, physiological and biosynthetic characteristics were investigated.
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Karsunkina N.P., N. P., E. V. Eremina E.V., and M. Yu Cherednichenko M.Yu. "Growth regulators in agriculture and biotechnology." In Растениеводство и луговодство. Тимирязевская сельскохозяйственная академия, 2020. http://dx.doi.org/10.26897/978-5-9675-1762-4-2020-56.

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The review is devoted to the history of the study of phytohormones and growth regulators. The features of the main classes of phytohormones and the prospects for their use in plant tissue and cell culture are also considered.
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Kuleshova, T. E., A. S. Galushko, N. R. Gall, and G. G. Panova. "Plant-microbial fuel cell with using the lettuce during cultivation by panoponic." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.139.

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Khizhnyak, E. I., N. N. Volchenko, A. A. Samkov, A. A. Khudokormov, and A. A. Lazukin. "Functioning of benthic type of microbial fuel cell with the possibility of utilization of toxic compounds." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.118.

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The electrogenesis of benthic-type microbial fuel cell was studied under conditions of pollutant contamination. The ability of microbial fuel cells to generate low-power electricity and participate in the biodegradation of toxic substances was revealed.
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Lobanova, L. P., and A. Yu Kolesova. "Variability of female gametophyte of tobacco in vivo and in vitro under the influence of extreme temperatures and its possible consequences." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.151.

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High and low temperatures induce the formation of abnormal embryo sacks (ES). ES with additional cells in the egg cell apparatus and synergids that are similar to an egg sells are capable of producing seeds with additional and haploid embrios.
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"The effect of "early"protein of papillomavirus HPV16 E2 made in plant expression system on the base of tomato fruit on tumor formation in mice infected with cancer HeLa cells." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-168.

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Reports on the topic "Plant cell biotechnology"

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Savaldi-Goldstein, Sigal, and Siobhan M. Brady. Mechanisms underlying root system architecture adaptation to low phosphate environment. United States Department of Agriculture, January 2015. http://dx.doi.org/10.32747/2015.7600024.bard.

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In order to advance our understanding towards potential biotechnology improvement of plant performance, we studied root responses to limited P in two different plants, Arabidopsis and tomato. Arabidopsis is among the most studied model plants that allows rapid application of molecular and developmental experiments while tomato is an important crop, with application in agriculture. Using Arabidopsis we found that steroid hormones modulate the extent of root elongation in response to limited P, by controlling the accumulation of iron in the root. We also found that the availability of P and iron control the activity of the steroid hormone in the root. Finally, we revealed the genes involved in this nutrient-hormone interaction. Hence, the ferroxidase LPR1 that promotes iron accumulation in response to low P is repressed by the transcription factor BES1/BZR1. Low P inhibits the steroid hormone pathway by enhancing the accumulation of BKI1. High levels of BKI1 inhibit the activity of the steroid hormone receptor at the cell surface and iron accumulation increases inside the root, resulting in a slow growth. Together, the extent of root elongation depends on interactions between an internal cue (steroid hormone) and cues derived from the availability of P and iron in the environment. Using tomato, we found that the response of two cultivated tomato varieties (M82 and New Yorker) to limited P is distinct from that of the wild species, Solanumpennellii. This is implicated at both the levels of root development and whole plant physiology. Specifically, while the root system architecture of cultivated tomato is modulated by limited P availability, that of the wild type species remained unaffected. The wild species appears to be always behaving as if it is always in phosphate deprived conditions, despite sufficient levels of phosphate. Hyper-accumulation of metals appears to mediate this response. Together, this knowledge will be used to isolate new genes controlling plant adaptation to limited P environment.
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Davis, Eric L., Yuji Oka, Amit Gal-On, Todd Wehner, and Aaron Zelcer. Broad-spectrum Resistance to Root-Knot Nematodes in Transgenic Cucurbits. United States Department of Agriculture, June 2013. http://dx.doi.org/10.32747/2013.7593389.bard.

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Root-knot nematodes (RKN), Meloidogyne spp., are extremely destructive pathogens of cucurbit crops grown in the United States and Israel. The safety and environmental concerns of toxic nematicides, and limited sources of natural cucurbit resistance to the four major species of Meloidogyne that threaten these crops in Israel and the U.S., have emphasized the use of biotechnology to develop cucurbits with novel RKN resistance. The U.S. scientists have identified over 40 unique RKN parasitism genes that encode nematode secretions involved in successful plant root infection by RKN, and they have demonstrated that expression of a double-stranded RNA (dsRNA) complementary to a RKN parasitism gene (called 16DIO) in Arabidopsis thaliana induced RNA interference (RNAi)-mediated silencing of the RKN16DlO gene and produced transgenic plants with strong resistance to all four major RKN species. The expression 8D05 parasitism gene was found to coincide with the timing of upregulation of NtCel7 promoter (identified to be upregulated in giantcells by US scientists). NtCel7 promoter was used to express the genes at the right time (early stages of infection) and in the right place (giant-cells) in transgenic plants. US partners produced NtCel7 (nematode-induced promoter)-driven 16DlO-RNAi and 8DOS-RNAi constructs, pHANNIBAL 4D03-RNAi construct and modified 16DlO-RNAi construct (for increased RNAi expression and efficacy) for cucurbit transformation in Israel. In Arabidopsis, some 16DlO-RNAi plant lines show greater levels of resistance to M. incognita than others, and within these lines resistance of greater than 90% reduction in infection is observed among almost all replicates in US. The level of observed nematode resistance is likely to be directly correlated with the level of RNAi expression in individual plants. In Israel, all the RKN parasitism genes-RNAi constructs were successfully transformed into cucumber and melon. The transgenic lines were evaluated for expression of the transgene siRNA in leaves and roots. Those displaying transgene siRNA accumulation were passed on for nematode resistance analysis. Rl seedlings from different lines were subjected to evaluation for resistance to M. javanica. None of the lines was resistant to the nematode in contrast with US partner's results in Arabidopsis. This could be for the following reasons: a) The level of transgene siRNA was insufficient in cucumber and tomato to cause resislance. b) 111e nemalode species on cucwnber IIlay be different ur act in a different manner. c) The assay was performed in soil with a high level of nematode inoculation, and not in petri dish, which may not permit the observation of a low level of resistance.
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Delmer, Deborah P., Douglas Johnson, and Alex Levine. The Role of Small Signal Transducing Gtpases in the Regulation of Cell Wall Deposition Patterns in Plants. United States Department of Agriculture, August 1995. http://dx.doi.org/10.32747/1995.7570571.bard.

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The combined research of the groups of Delmer, Levine and Johnson has led to a number of interesting findings with respect to the function of the small GTPase Rac in plants and also opened up new leads for future research. The results have shown: 1) The Rac13 protein undergoes geranylgeranlyation and is also translocated to the plasma membrane as found for Rac in mammals; 2) When cotton Rac13 is highly- expressed in yeast, it leads to an aberrant phenotype reminiscent of mutants impaired in actin function, supporting a role for Rac13 in cytoskeletal organization; 3) From our searches, there is no strong evidence that plants contain homologs of the related CDC42 genes found in yeast and mammals; 4) We have identified a rather unique Rac gene in Arabidopsis that has unusual extensions at both the N- and C-terminal portions of the protein; 5) New evidence was obtained that an oxidative burst characterized by substantial and sustained production of H202 occurs coincident with the onset of secondary wall synthesis in cotton fibers. Further work indicates that the H202 produced may be a signal for the onset of this phase of development and also strongly suggests that Rac plays an important role in signaling for event. Since the secondary walls of plants that contain high levels of lignin and cellulose are the major source of biomass on earth, understanding what signals control this process may well in the future have important implications for manipulating the timing and extent of secondary wall deposition. 6) When the cotton Rac13 promoter is fused to the reporter gene GUS, expression patterns in Arabidopsis indicate very strong and specific expression in developing trichomes and in developing xyelm. Since both of these cell types are engaged in secondary wall synthesis, this further supports a role for Rac in signaling for onset of this process. Since cotton fibers are anatomically defined as trichomes, these data may also be quite useful for future studies in which the trichomes of Arabidopsis may serve as a model for cotton fiber development; the Rac promoter can therefore be useful to drive expression of other genes proposed to affect fiber development and study the effects on the process; 7) The Rac promoter has also been shown to be the best so far tested for use in development of a system for transient transformation of developing cotton fibers, a technique that should have many applications in the field of cotton biotechnology; 8) One candidate protein that may interact with Rac13 to be characterized further in the future is a protein kinase that may be analogous to the PAK kinase that is known to interact with Rac in mammals.
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Elroy-Stein, Orna, and Dmitry Belostotsky. Mechanism of Internal Initiation of Translation in Plants. United States Department of Agriculture, December 2010. http://dx.doi.org/10.32747/2010.7696518.bard.

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Original objectives Elucidation of PABP's role in crTMV148 IRES function in-vitro using wheat germ extract and krebs-2 cells extract. Fully achieved. Elucidation of PABP's role in crTMV148 IRES function in-vivo in Arabidopsis. Characterization of the physical interactions of PABP and other potential ITAFs with crTMV148 IRES. Partly achieved. To conduct search for additional ITAFs using different approaches and evaluate the candidates. Partly achieved. Background of the topic The power of internal translation via the activity of internal ribosomal entry site (IRES) elements allow coordinated synthesis of multiple gene products from a single transcription unit, and thereby enables to bypass the need for sequential transformation with multiple independent transgenes. The key goal of this project was to identify and analyze the IRES-trans-acting factors (ITAFs) that mediate the activity of a crucifer-infecting tobamovirus (crTMV148) IRES. The remarkable conservation of the IRES activity across the phylogenetic spectrum (yeast, plants and animals) strongly suggests that key ITAFs that mediate its activity are themselves highly conserved. Thus, crTMV148 IRES offers opportunity for elucidation of the fundamental mechanisms underlying internal translation in higher plants in order to enable its rational manipulation for the purpose of agricultural biotechnology. Major conclusions and achievements. - CrTMV IRES requires PABP for maximal activity. This conclusion was achieved by PABP depletion and reconstitution of wheat germ- and Krebs2-derived in-vitro translation assays using Arabidopsis-derived PABP2, 3, 5, 8 and yeast Pab1p. - Mutations in the internal polypurine tract of the IRES decrease the high-affinity binding of all phylogenetically divergent PABPs derived from Arabidopsis and yeast in electro mobility gel shift assays. - Mutations in the internal polypurine tract decrease IRES activity in-vivo. - The 3'-poly(A) tail enhances crTMV148 IRES activity more efficiently in the absence of 5'-methylated cap. - In-vivo assembled RNPs containing proteins specifically associated with the IRES were purified from HEK293 cells using the RNA Affinity in Tandem (RAT) approach followed by their identification by mass spectroscopy. - This study yielded a list of potential protein candidates that may serve as ITAFs of crTMV148 IRES activity, among them are a/b tubulin, a/g actin, GAPDH, enolase 1, ribonuclease/angiogenin inhibitor 1, 26S proteasome subunit p45, rpSA, eEF1Bδ, and proteasome b5 subunit. Implications, both scientific and agriculture. The fact that the 3'-poly(A) tail enhances crTMV148 IRES activity more efficiently in the absence of 5'-methylated cap suggests a potential joint interaction between PABP, the IRES sequence and the 3'-poly(A). This has an important scientific implication related to IRES function in general.
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Stern, David, and Gadi Schuster. Manipulation of Gene Expression in the Chloroplast. United States Department of Agriculture, September 2000. http://dx.doi.org/10.32747/2000.7575289.bard.

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The steady-state level of a given mRNA is determined by its rates of transcription and degradation. The stabilities of chloroplast mRNAs vary during plant development, in part regulating gene expression. Furthermore, the fitness of the organelle depends on its ability to destroy non-functional transcripts. In addition, there is a resurgent interest by the biotechnology community in chloroplast transformation due to the public concerns over pollen transmission of introduced traits or foreign proteins. Therefore, studies into basic gene expression mechanisms in the chloroplast will open the door to take advantage of these opportunities. This project was aimed at gaining mechanistic insights into mRNA processing and degradation in the chloroplast and to engineer transcripts of varying stability in Chlamydomonas reinhardtii cells. This research uncovered new and important information on chloroplast mRNA stability, processing, degradation and translation. In particular, the processing of the 3' untranslated regions of chloroplast mRNAs was shown to be important determinants in translation. The endonucleolytic site in the 3' untranslated region was characterized by site directed mutagensis. RNA polyadenylation has been characterized in the chloroplast of Chlamydomonas reinhardtii and chloroplast transformants carrying polyadenylated sequences were constructed and analyzed. Data obtained to date suggest that chloroplasts have gene regulatory mechanisms which are uniquely adapted to their post-endosymbiotic environment, including those that regulate RNA stability. An exciting point has been reached, because molecular genetic studies have defined critical RNA-protein interactions that participate in these processes. However, much remains to be learned about these multiple pathways, how they interact with each other, and how many nuclear genes are consecrated to overseeing them. Chlamydomonas is an ideal model system to extend our understanding of these areas, given its ease of manipulation and the existing knowledge base, some of which we have generated.
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