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Dissertations / Theses on the topic 'Plant enzymes – Analysis'

Author: Grafiati

Published: 4 June 2021

Last updated: 4 February 2022

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1

Shamandi, Nahid. "Functional analysis of plant RNaseIII enzymes." Thesis, Paris 11, 2013. http://www.theses.fr/2013PA112157/document.

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Chez la majorité des eucaryotes, les petits ARN (miRNA et siRNA) jouent des rôles essentiels au cours du développement, dans les réponses adaptatives aux stress, et dans la maintenance de la stabilité génétique. Les plantes codent quatre enzymes RNaseIII de type DICER-LIKE (DCL). DCL1, produit les miRNAs, tandis que DCL2, DCL3 et DCL4 produisent des siRNAs des tailles diverses. Les plantes codent également des enzymes appelées RNASE-THREE-LIKE (RTL) auxquelles il manque certains domaines spécifiques aux DCLs, et dont la fonction est largement inconnue.Des plantes sur-exprimant RTL1 montrent de

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2

Samodien, Mugammad Ebrahim. "Analysis of enzymes involved in starch phosphate metabolism." Thesis, Stellenbosch : University of Stellenbosch, 2009. http://hdl.handle.net/10019.1/2633.

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Thesis (MSc (Genetics. Plant Biotechnology)) --University of Stellenbosch, 2009.<br>ENGLISH ABSTRACT: This project examined the role of proteins in starch phosphate metabolism. The first part was aimed at the functional characterization of the SEX4, LSF1 and LSF2 genes in both plants and bacteria. Constructs were produced to allow for expression of the three proteins in E. coli with the SEX4 and LSF2 proteins being successfully purified and used to produce antibodies. Immunoblot analysis indicated that the antibodies recognised the repective proteins in extracts, but it was not clear if t

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3

Duncanson, Euan. "Biochemical and mutant analysis of nitrite reduction in barley." Thesis, University of St Andrews, 1991. http://hdl.handle.net/10023/14465.

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The object of this study was to isolate and characterise barley mutants that lacked a functional nitrite reductase activity. This work should complement previous studies on nitrate reductase to develop a fuller understanding of nitrate assimilation in barley. 30 nitrite reduction-deficient M2 barley plants (azide-treated in the M1) were identified as nitrite accumulators after treatment with nitrate. Biochemical analysis M2 selections revealed that leaf tissue from 9 selected plants lacked detectable nitrite reductase protein. Progeny from 4 of the selected plants (Golden Promise 2406, Tweed 3

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4

Mopai, Kgaugelo Lydia. "Screening, purification and characterisation of an active Hydroxynitrile Lyase (Nitrilase) from indigenous South African Plants." Thesis, University of Limpopo, 2013. http://hdl.handle.net/10386/1334.

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Thesis (MSc. (Biochemistry)) -- University of Limpopo, 2013<br>Hydroxynitrile lyases (HNLs) are enzymes that catalyse enantioselective cleavage of the substrate in a reaction and are also used as important industrial biocatalysts for the synthesis of chiral cyanohydrins. The aim of the study was to screen indigenous South African plants for potential hydroxynitrile lyase activity, purify and biochemically characterise the active hydroxynitrile lyase(s) from the selected plants. Several indigenous plants were randomly collected, identified and screened for HNL activity. The plant parts (leaves,

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5

Cotton, Kimberly Lynn. "Genetic and biochemical analysis of essential enzymes in triacylglycerol synthesis in arabidopsis." Thesis, Washington State University, 2016. http://pqdtopen.proquest.com/#viewpdf?dispub=10043101.

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<p> Plant oils are used in food, fuel, and feedstocks for many consumer products, and so understanding the process by which they are made and modified will help us to make plant oils more healthy, useful, and sustainable. While some of the genes encoding the ER-localized enzymatic steps to triacylglycerol (TAG) have been well understood and documented, several are still in need of study. The glycerol-3-phosphate acyl transferase (GPAT) enzymatic activity is the first step in the pathway to TAG, and it acylates glycerol 3-phosphate to produce lysophosphatidic acid. <i>GPAT9</i> (AT5G60620) is c

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6

Wen, Miao. "Chemical analysis and biosynthesis of secondary alcohols in plant cuticular waxes." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/4304.

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The biosynthesis of wax components containing secondary functional groups was investigated in the current study. Two fundamentally different pathways were proposed to introduce the secondary functional groups. One pathway involves hydroxylation of elongated substrates. Wax components characterized by two functional groups located on or near the centre of the carbon chain, nonacosane-14,15-diol, -14,16-diol and -13,15-diol as well as corresponding ketols were identified for the first time in Arabidopsis stem wax. The alkanediols and ketols were dominated by the C-14,15 isomers. The absence of a

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7

Pisarczyk, Elizabeth W. "The effect of garlic mustard (Alliaria petiolata) density on soil nutrient availability and microbial enzyme activity in Northwest Ohio : a gradient analysis /." Connect to full text in OhioLINK ETD Center, 2009. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=toledo1260236506.

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Thesis (M.S.)--University of Toledo, 2009.<br>Typescript. "Submitted as partial fulfillment of the requirements for The Master of Science Degree in Biology (Ecology-track)." "A thesis entitled"--at head of title. Bibliography: leaves 28-32.

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8

Devaiah, Shivakumar P., Cheng Zhang, and Cecelia A. McIntosh. "Structure and Functional Analysis of Glucosyltransferase from Citrus paradisi." Digital Commons @ East Tennessee State University, 2014. https://dc.etsu.edu/etsu-works/344.

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Glucosyltransferases (GTs) are enzymes that expedite the incorporation of UDP-activated glucose to a corresponding acceptor molecule. This enzymatic reaction stabilizes structures and affects solubility, transport, and bioavailability of flavonoids for other metabolic processes. Flavonoid glycosides affect taste characteristics in citrus making the associated glucosyltransferases particularly interesting targets for biotechnology applications. Custom design of enzymes requires understanding of structure/function of the protein. The present study focuses on creating mutant flavonol-3-O-glucosyl

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9

Sathanantham, Preethi, Shiva K. Devaiah, and Cecelia A. McIntosh. "Structure-Function Analysis of Grapefruit Glucosyltransferase Protein – Identification of Key Amino Acid Residues for its Rigid Substrate Specificity." Digital Commons @ East Tennessee State University, 2015. https://dc.etsu.edu/etsu-works/352.

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Flavonoids are an important class of secondary metabolites widely distributed in plants. The majority of naturally occurring flavonoids are found in glucosylated form. Glucosyltransferases are enzymes that enable transfer of glucose from an activated donor (UDP-glucose) to the acceptor flavonoid substrates. A flavonol specific glucosyltransferase cloned from Citrus paradisi (Cp3OGT) has strict substrate and regiospecificity. In this study, amino acid residues that could potentially alter the rigidity observed in this enzyme were mutated to position equivalent residues of a putative anthocyanin

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10

Ahmadian, Hossein. "Analysis of enzyme dust formation in detergent manufacturing plant." Thesis, University of Leeds, 2008. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.515281.

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11

Bulegon, Lucas Guilherme. "Relações hídricas, trocas gasosas e atividade enzimática de Urochloa ruziziensis inoculada com Azospirillum brasilense, sob déficit hídrico." Universidade Estadual do Oeste do Paraná, 2016. http://tede.unioeste.br:8080/tede/handle/tede/1300.

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Made available in DSpace on 2017-07-10T17:37:13Z (GMT). No. of bitstreams: 1 Lucas Guilherme Bulegon.pdf: 2078794 bytes, checksum: ccb242a3255f730bf9153212913cc026 (MD5) Previous issue date: 2016-02-19<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>The water deficit causes the plant physiological and biochemical disarray, so is sought ways to reduce these effects and increase the tolerance of plants to water stress. Thus, the present study aimed to evaluate the physiological behavior and biochemical Urochloa ruziziensis inoculated with Azospirillum brasilense via seed an

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12

Pires, Tatiana da Costa Raposo. "Alterações pós-colheita em raízes de mandioquinha-salsa (Arracacia xanthorrhiza Bancroft.): atividade enzimática, identificação de contaminante e caracterização parcial do amido." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/9/9131/tde-29082017-122147/.

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Este trabalho teve como principais objetivos verificar as alterações na atividade de enzimas amilolíticas, pectinolíticas e celulásicas em raízes de mandioquinha-salsa durante o período pós-colheita, sob diferentes condições de armazenamento, visando avaliar os mecanismos de deterioração das raízes, bem como identificar o microrganismo possivelmente responsável pela alta perecibilidade das raízes. Além disso, foram estudadas características físico-químicas e reológicas do amido de mandioquinha extraído em laboratório. Para a detecção de atividade pectinesterásica (PE) e poligalacturonásica (PG

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13

Fischer, Juliane [Verfasser], Birgit [Akademischer Betreuer] Dräger, Wolfgang [Akademischer Betreuer] Brandt, and Dietrich [Akademischer Betreuer] Ober. "Homology modelling, virtual screening and evolutionary analyses of plant enzymes metabolising putrescine / Juliane Fischer. Betreuer: Birgit Dräger ; Wolfgang Brandt ; Dietrich Ober." Halle, Saale : Universitäts- und Landesbibliothek Sachsen-Anhalt, 2015. http://d-nb.info/1077768095/34.

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14

Petsalo, A. (Aleksanteri). "Development of LC/MS techniques for plant and drug metabolism studies." Doctoral thesis, Oulun yliopisto, 2011. http://urn.fi/urn:isbn:9789514294419.

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Abstract Liquid chromatography (LC) combined with mass spectrometry (MS) is a powerful tool for qualitative and quantitative analytics of organic molecules from various matrices, and the use of this hyphenated technique is very common in bioanalytical laboratories. In this study, LC/MS methods and the required sample preparation applications were developed for plant flavonoid and drug metabolism studies. The main focus was in developing methods to be used during cytochrome P450 (CYP) -specific drug interaction studies. Traditional high performance liquid chromatography (HPLC) and new, more efﬁ

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15

Silva, Franklin Magnum de Oliveira. "Integrative analyses of photosynthesis, plant growth, metabolite levels and enzyme activities in an introgression line population of Solanum pennellii." Universidade Federal de Viçosa, 2016. http://www.locus.ufv.br/handle/123456789/21421.

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Submitted by MARCOS LEANDRO TEIXEIRA DE OLIVEIRA (marcosteixeira@ufv.br) on 2018-08-24T12:59:01Z No. of bitstreams: 1 texto completo.pdf: 3178737 bytes, checksum: 5041c62a2f0856a630f7f0f0865ee43b (MD5)<br>Made available in DSpace on 2018-08-24T12:59:01Z (GMT). No. of bitstreams: 1 texto completo.pdf: 3178737 bytes, checksum: 5041c62a2f0856a630f7f0f0865ee43b (MD5) Previous issue date: 2016-08-12<br>Fundação de Amparo à Pesquisa do Estado de MInas Gerais<br>Para identificar regiões genômicas envolvidas na regulação de processos fisiológicos fundamentais, como fotossíntese, respiração e aqu

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16

Muir, Ryann Morgan. "Analysis of gallic acid production by the bi-functional enzyme shikimate-5-dehydrogenase in higher plants and bacteria /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.

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17

Bryant, Beverley Ann. "The production and analysis of tobacco plants with reduced levels of the Calvin cycle enzyme sedoheptulose-1,7-bisphosphatase." Thesis, University of Essex, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310052.

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18

Gomes, Carolina Nogueira Carvalho. "Production and analysis of pharmaceutically relevant peptides in Lactuca sativa and Medicago truncatula." Master's thesis, Universidade de Aveiro, 2015. http://hdl.handle.net/10773/21655.

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Mestrado em Biotecnologia Alimentar<br>O molecular pharming permite a produção de proteinas terapêuticas recombinantes a larga escala, de forma segura e a baixo custo. No presente trabalho, é proposta a produção heteróloga de quatro péptidos inibidores da ACE em dois emergentes sistemas de expressão vegetal, Lactuca sativa (alface) e Medicago truncatula (luz cortada). A utilização da alface, uma planta comestível, pode proporcionar um meio para a administração oral de péptidos anti-hipertensivos, criando um novo alimento funcional. Por outro lado, a utilização de M. truncatula, uma le

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19

Slaughter, Lindsey C. "SOIL MICROBIAL COMMUNITY RESPONSE TO CLIMATE CHANGE: RESULTS FROM A TEMPERATE KENTUCKY PASTURE." UKnowledge, 2012. http://uknowledge.uky.edu/pss_etds/8.

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Climate change is likely to alter plant species composition and interactions between plants and soil microbes that together dictate the quantity and quality of forage produced in pastures, the base of animal production in central Kentucky. This study assessed the seasonal dynamics of soil microbes and their response to increased temperature (+3oC) and growing season precipitation (+30% of the mean annual). Total soil microbial biomass, community composition, enzyme activities, potential carbon mineralization, and catabolic responses to selected substrates were measured seasonally in the differ

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20

Leonardo, Marcelo [UNESP]. "Produção de óleo essencial associado à deficiência hídrica em plantas Ocimum basilicum L. cv. genovese." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/103422.

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Made available in DSpace on 2014-06-11T19:32:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-03-05Bitstream added on 2014-06-13T18:44:01Z : No. of bitstreams: 1 leonardo_m_dr_botfca.pdf: 849833 bytes, checksum: 14dfc79b16640209ce661eb409949474 (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Universidade Estadual Paulista (UNESP)<br>Um dos mais importantes fatores ambientais e agronômicos que afetam a produção de metabólitos secundários em plantas é a água. Quantidade limitada de água tem efeito negativo sobre o crescimento e desenvolvimento das plan

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21

Ronconi, Silvia [Verfasser], Ulrich [Akademischer Betreuer] Genschel, and Alfons [Akademischer Betreuer] Gierl. "The Pantothenic Acid and Coenzyme A Pathway in Plants and Archaea : Analysis of Metabolite Levels in Arabidopsis thaliana and Characterization of Archaeal Enzymes for the Synthesis of 4'-phosphopantetheine / Silvia Ronconi. Gutachter: Alfons Gierl. Betreuer: Ulrich Genschel." München : Universitätsbibliothek der TU München, 2006. http://d-nb.info/1058141473/34.

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22

Leonardo, Marcelo 1974. "Produção de óleo essencial associado à deficiência hídrica em plantas Ocimum basilicum L. cv. genovese /." Botucatu : [s.n.], 2007. http://hdl.handle.net/11449/103422.

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Orientador: Fernando Broetto<br>Banca: Roberto Lyra Villas Bôas<br>Banca: Magali Ribeiro da Silva<br>Banca: Marcia Ortiz Mayo Marques<br>Banca: Otto Jesu Crocomo<br>Resumo: Um dos mais importantes fatores ambientais e agronômicos que afetam a produção de metabólitos secundários em plantas é a água. Quantidade limitada de água tem efeito negativo sobre o crescimento e desenvolvimento das plantas. Entretanto, a deficiência hídrica (DH), muitas vezes tem se mostrado positiva no acúmulo de constituintes ativos em espécies medicinais, aromáticas e condimentares. Verificou-se a influência da DH na p

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23

Dubald, Manuel. "L'exopolygalacturonase de maïs : caractérisation de la protéine, et analyse par transgénèse du promoteur d'un gène codant pour cette enzyme." Université Joseph Fourier (Grenoble), 1993. http://www.theses.fr/1993GRE10187.

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L'exopolygalacturonase (exopg) est une enzyme pectolytique particulierement abondante dans le pollen mature de mais. Les genes exopg sont exprimes tardivement au cours du developpement du gametophyte male. L'enzyme n'est detectee qu'apres la completion des mitoses polliniques, puis est synthetisee en quantites croissantes jusqu'a la fin de la microsporogenese. Ce profil d'expression suggere que l'exopg joue un role au moment de la germination et de la croissance du tube pollinique. Deux isoformes de 49 et 53 kda ont ete identifiees et caracterisees dans une fraction purifiee a partir de pollen

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24

Bory, Sothavireak. "Étude ethnopharmacologique d'une plante utilisée en médecine traditionnelle cambodgienne dans le traitement de la malaria : stephania rotunda (Lour.) : approche analytique, phytochimique, pharmacologique et métabolique." Thesis, Aix-Marseille 2, 2010. http://www.theses.fr/2010AIX22960.

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La mise au point et la validation d’une méthode de dosage par CLHP des alcaloïdes majoritaires (cépharanthine,xylopinine, tétrahydropalmatine) de Stephania rotunda sont réalisées. La méthode est appliquée au dosage des alcaloïdesdans différentes parties de la plante. Le tubercule est la drogue végétale la plus riche en alcaloïdes majoritaires, enparticulier en cépharanthine (1%). Le dosage des alcaloïdes majoritaires des tubercules d’origine différente est effectué.La cépharanthine est beaucoup plus concentrée dans le tubercule de Pailin (1,9%). La comparaison de la compositionchimique du tube

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25

Lin, Yu-Chein, and 林裕傑. "Analysis of functional ingredients of commercial plant-based enzyme products." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/57518402430453864240.

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碩士<br>國立中興大學<br>食品暨應用生物科技學系所<br>97<br>Abstract In recent years, with the enhancement of health consciousness in consumers and health knowledge advocated by nutritionist have provided people with the concepts not only to eat full, but also to eat healthy. Attention to the health concept, change in living habits and diets, and the progress in alternative medicine and life science research impel the vigorous development of the health food industry. The health food products contain the specific ingredients and possess physiological functions or can demonstrate the food of the health efficiency. Am

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26

Ward, Nagib M. "Reverse-phase high performance liquid chromatography analyses of grape (Vin vinifera) seed extracts for the presence and activity of the proanthocyanidin condensing enzyme." 2007. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:1449689.

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27

Shie, Han-Yu, and 謝邯宇. "Structural analysis and functional improvement of plant polysaccharides degrading enzyme from Clostridium thermocellum." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/84386020550022089559.

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碩士<br>國立臺灣大學<br>生化科學研究所<br>102<br>Part1:The glycoside hydrolase 5 (GH5) family contains more than 3000 prokaryotic and eukaryotic enzymes with a large variety of specificities, including endoglucanses, cellobiohydrolases, chitosanases, mannanases and xylanases. Two GH5 enzymes, CtCel5E and TmCel5A, possess different bi-functional activities, cellulase/xylanase and cellulase/mannanase, respectively, although they share sequence homology. The amino acid sequences of these two enzymes are aligned based on SCHEMA and a block was found significantly different in sequence. As revealed by the two pro

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