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DANSOU, Christian C., Julienne KUISEU, Géraldo T. HOUMENOU, et al. "Improved Traditional Drugs: State of knowledge for the sustainable management of gastrointestinal parasitic nematodes of small ruminants." Journal of Drug Delivery and Therapeutics 11, no. 2-S (2021): 205–10. http://dx.doi.org/10.22270/jddt.v11i2-s.4811.
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This article aims to present the current state of the fight against gastrointestinal parasites in small ruminants from Improved Traditional Drugs (DTI). A host of scientific documents were consulted by means of search engines to gather the information useful for this synthesis. The failure of conventional treatments has led to the use of new methods to treat digestive pathologies due to internal parasitism in herds of sheep and goats. These include, among others, herbal medicine, homeopathy, aromatherapy, the mixed grazing system for small and large ruminants, ... All plant parts of plants can be used in the preparation of remedies. Several scientific studies show that plants are full of chemical compounds that cause anthelmintic effects on gastrointestinal parasites in small ruminants. Literature searches have revealed that studies on DTIs in the management of gastrointestinal nematodes in small ruminants are non-existent. In addition to the forms (decocted, macerated, infused, etc.) traditionally used by breeders, there are other forms that can be used to make DTIs from plant extracts. There are capsules, capsules, tablets etc. The placing on the market of DTIs requires official authorization based on the safety and reproducibility of the products. From the documents consulted, it appears that scientific research has not yet focused on the formulation of DTIs for the treatment of gastrointestinal parasitic nematodes in small ruminants. It will be useful to manufacture them to facilitate access to veterinary care and the sustainable management of animal health.
 Keywords: Improved Traditional Drugs; Gastrointestinal nematodes; Small ruminants, West Africa; Central Africa.
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Novosaduyk, Tatiana Vladimirovna, Victoria Tsvetkova, Victoria Tsvetkova, Anatolii Komissarenko, and Anatolii Komissarenko. "The potential of homeopathic phenomenon in plant growth." International Journal of High Dilution Research - ISSN 1982-6206 14, no. 2 (2021): 11–12. http://dx.doi.org/10.51910/ijhdr.v14i2.790.
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For over two centuries homeopathy has demonstrated its therapeutic properties, thus gaining more and more supporters worldwide. Many researchers opine that due to absence of a medicinal substance in a homeopathic preparation it is not necessary to study its healing effect. However, for the last few years our research was focused not only on understanding the mechanism(s) of homeopathic action in medical and veterinary practices but also on the effect of different homeopathic preparations on plants. The studies aimed to rule out the placebo effect from the homeopathic phenomenon, but also at identification of therapeutic effect on different plants. These studies allowed to extend the boundaries of practical use of homeopathy including its common use in the farming industry.
 The efficacy of various homeopathic preparations was assessed for their use in plant growth through application techniques such as watering, spraying, soaking and sprinkling etc. Growth dynamics, duration of fruit ripening, crop yield were monitored and compared to reference plants. Homeopathic preparations for agricultural plants were carefully selected on the basis of their effect on the crop yield – the principal criterion for the selection.
 Based on these criteria it was observed that the homeopathic picture of tomatoes, cucumbers, potatoes is almost same within one variety, in all tested sorts, although, in case of fruit trees such as apple, pear the homeopathic similimum varied depending of the selection method.
 Our results showed that, in case of trees, one-time application of homeopathic preparation during a season is preferable and sufficient. In case of vegetable cultures, application may not be more frequent than once a month as more frequent application results in diminishing of yield. For root plants single treatment at the time of planting was sufficient.
 The performed studies showed that the growth and ripening indicators significantly prevailed in the vegetables, which had been treated with homeopathic preparations, as compared to common traditional way of plant handling (controls).
 Thus the performed studies suggest the following:
 a) Homeopathic effect on biological structures is not a placebo effect.
 b) Obtained data are a categorical proof of the homeopathic preparation effect on plants causing higher yield in agricultural crops and therefore, have good potential for their use on the industrial scale in agriculture.
 c) The potential of the homeopathic phenomenon practical use has not been fully explored yet and needs a follow-up study.
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Carvalho, Aloisio Cunha de, and Leoni Villano Bonamin. "Viscum album (L) extracts in cancer treatment: a systematic review of in vitro and in vivo studies." International Journal of High Dilution Research - ISSN 1982-6206 14, no. 2 (2021): 52. http://dx.doi.org/10.51910/ijhdr.v14i2.787.
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Background: Several reviews about phytotherapy and homeopathy have been published in the last years, including Viscum album (VA.L). VA is a parasite plant whose extract has anti-cancer proprieties and is used alone or in combination with conventional chemotherapy. 
 Methods: We performed a systematic review about the in vivo and in vitro models described in the literature, including veterinary clinical trials. The literature was consulted from Pubmed database.
 Results: There are several kinds of pharmaceutical preparations about VA and their active principles used in experimental studies, lectin being frequently studied (alone or as an extract compound). More than 50% of available literature about VA is related to the lectin effects. On the other hand, the effects of viscotoxins are less studied. Among the in vivo experimental studies about VA and its compounds, the B16 murine melanoma is the most used model, followed by Ehrlich, Walker and Dalton tumors. The results point to the apoptotic effects, metastasis control and tumor regression. Some veterinary clinical studies about the use of VA in the treatment of sarcoid, fibrosarcoma and neuroblastoma are quoted in literature too, with interesting results. Considering the in vitro models, our review revealed that NALM6 leukemia cells, B16 melanoma and NC1-H460 lung carcinoma were the most studied tumor models, apoptosis signals being the most important findings. Only one study verified immunoglobulin and interleukin production. All consulted papers were related to phytotherapy preparations only.
 Conclusions: Although the literature about the anti-cancer activity of VA extract and its lectins is enough, there is a marked lack of information about viscotoxin activities and about the effects of homeopathic preparations of this plant on animal tumors and on in vitro cultivated tumor cells.
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Jawhari, Fatima Zahra, Abdelfattah El Moussaoui, Hamada Imtara, et al. "Evaluation of the acute toxicity of the extracts of Anacyclus pyrethrum var. pyrethrum (L.) and Anacyclus pyrethrum var. depressus Maire in Swiss mice." Veterinary World 14, no. 2 (2021): 457–67. http://dx.doi.org/10.14202/vetworld.2021.457-467.
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Background and Aim: Anacyclus pyrethrum (L.) has been used in traditional North African and Indian medicine for the treatment of several diseases such as cancer, rheumatism, epilepsy, diabetes, and Alzheimer's disease. Despite its medical benefits, few studies have examined its toxicity. The present study evaluated the acute toxicity of hydroethanolic extracts of different parts (roots, seeds, leaves, and capitula) of two varieties of A. pyrethrum (L.), namely, A. pyrethrum var. pyrethrum (L) and A. pyrethrum var. depressus (Ball) Maire, in mice. Materials and Methods: Acute toxicity was evaluated after the oral administration of different extracts at doses of 300, 500, and 2000 mg/kg. Mortality, body weight, general behavior, and adverse effects were observed daily for 14 days. At the end of the experiment, mice were sacrificed, and biochemical parameters and histopathology of the liver, kidneys, and spleen were analyzed. Results: The extracts of different parts of both plants induced no signs of toxicity or mortality during the observation period, excluding capitulum and seed extracts, which induced slight sedation at a dose of 2000 mg/kg. The LD50 of the extracts was estimated to exceed 2000 mg/kg. The administration of A. pyrethrum var. pyrethrum roots at a dose of 300 mg/kg resulted in significantly increased AST levels. However, the A. pyrethrum var. depressus root extract induced significant increases in the levels of both transaminases (alanine aminotransferase [ALT] and aspartate aminotransferase [AST]). The remaining extracts of both plants at a dose of 500 mg/kg significantly increased AST levels. Moreover, all plant extracts excluding the A. pyrethrum var. pyrethrum capitulum extract at 2000 mg/kg provoked significant increases in AST levels, and A. pyrethrum var. depressus roots provoked a significant increase of ALT levels. Meanwhile, mice treated with high doses of extracts (2000 mg/kg) displayed histopathological changes in the liver, kidneys, and spleen characterized by hepatic distress, inflammatory infiltration, focal tubular necrosis, vascular congestion, and lymphoid hyperplasia. Conclusion: The results of the present study indicate that the hydroethanolic extracts of different parts of two varieties of A. pyrethrum (L.) were not toxic in mice at low concentrations, whereas some toxic effects were detected in mice treated at 2000 mg/kg.
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Félix-Silva, Juliana, Raquel Brandt Giordani, Arnóbio Antonio da Silva-Jr, Silvana Maria Zucolotto, and Matheus de Freitas Fernandes-Pedrosa. "Jatropha gossypiifoliaL. (Euphorbiaceae): A Review of Traditional Uses, Phytochemistry, Pharmacology, and Toxicology of This Medicinal Plant." Evidence-Based Complementary and Alternative Medicine 2014 (2014): 1–32. http://dx.doi.org/10.1155/2014/369204.
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Jatropha gossypiifoliaL. (Euphorbiaceae), widely known as “bellyache bush,” is a medicinal plant largely used throughout Africa and America. Several human and veterinary uses in traditional medicine are described for different parts and preparations based on this plant. However, critical reviews discussing emphatically its medicinal value are missing. This review aims to provide an up-to-date overview of the traditional uses, as well as the phytochemistry, pharmacology, and toxicity data ofJ. gossypiifoliaspecies, in view of discussing its medicinal value and potential application in complementary and alternative medicine. Pharmacological studies have demonstrated significant action of different extracts and/or isolated compounds as antimicrobial, anti-inflammatory, antidiarrheal, antihypertensive, and anticancer agents, among others, supporting some of its popular uses. No clinical trial has been detected to date. Further studies are necessary to assay important folk uses, as well as to find new bioactive molecules with pharmacological relevance based on the popular claims. Toxicological studies associated with phytochemical analysis are important to understand the eventual toxic effects that could reduce its medicinal value. The present review provides insights for future research aiming for both ethnopharmacological validation of its popular use and its exploration as a new source of herbal drugs and/or bioactive natural products.
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Roosdiana, Anna, Fajar Shodiq Permata, Riera Indah Fitriani, Khairul Umam, and Anna Safitri. "Ruellia tuberosa L. Extract Improves Histopathology and Lowers Malondialdehyde Levels and TNF Alpha Expression in the Kidney of Streptozotocin-Induced Diabetic Rats." Veterinary Medicine International 2020 (October 10, 2020): 1–7. http://dx.doi.org/10.1155/2020/8812758.
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Ruellia tuberosa L. is a therapeutic plant that is generally consumed in Indonesian traditional medicine to prevent or cure various illnesses, i.e., diabetes. The current study was conducted to investigate the effects of hydroethanolic root extracts of Ruellia tuberosa L. on the kidney of streptozotocin-induced diabetic Wistar rats. In this study, male Wistar rats were divided into 5 groups: healthy rats (group 1), diabetic rats (group 2), and treated rats which received extract at dosages of 250 (group 3), 375 (group 4), and 500 (group 5) mg/kg body weight for 21 days. Diabetes mellitus was experimentally induced by the administration of five doses of streptozotocin 20 mg/kg body weight within five consecutive days. Significant increases in the value of TNF alpha expression and malondialdehyde (MDA) levels were observed in streptozotocin-induced diabetes rats. Furthermore, severe histological alterations of kidney tissues occurred in the diabetic rats group. After treatment was applied, the value of TNF alpha expression and MDA levels on the kidney decreased considerably p < 0.05 in groups 3, 4, and 5. The optimum dosage was obtained at a dose of 250 mg/kg body weight (group 3), which had 42.24% and 52.70% decrease in TNF alpha expression and MDA levels, respectively. The histopathological profiles of the kidney also showed significant improvements in treated groups. The most prominent recoveries were also shown in group 3. The treatments induced repairment in the glomerular and renal tubular damages in the kidney tissues. To conclude, these results emphasize potentially health valuable properties of hydroethanolic root extracts of R. tuberosa L. in rats with streptozotocin-induced diabetes.
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Davidovic, V., Joksimovic Todorovic, B. Stojanovic, and R. Relic. "Plant usage in protecting the farm animal health." Biotehnologija u stocarstvu 28, no. 1 (2012): 87–98. http://dx.doi.org/10.2298/bah1201087d.
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Phytotherapy, as form of traditional and conventional veterinary medicine, includes curing, alleviation and prevention the onset of diseases in animals based on natural medicinal raw materials and folk remedies made from plants. In animal health care, the use of medicinal and other plants as supporting therapy, in preventive purposes or as a complete therapy has a large potential regardless whether it is the question of individual or combined plant medicinal preparations which act synergistically. Phytotherapy is very intensively used in prophylactic purposes and with the aim of treatment of milder forms of diseases, chronic diseases and recurrent infections as well as in organic livestock production. Extracts and preparations from different plants are used with the aim of intensifying the activity of immune system and stimulations some functions of organism. The great number of herb metabolites shows antimicrobial and antiparasitic activity and gives the positive effects in the treatment of ectoparasitoses and endoparasitoses. This research paper gives a review of the plants most frequently used in ethnoveterinary medicine for health purposes of farm animals.
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Sidi, Irvine Yèinou Minaflinou Sacca, Géorcelin Goué Alowanou, Esaïe Tchétan, Maliki Youssouf Aminou, Sylvie Mawulé Hounzangbé-Adoté, and Séverin Babatoundé. "Effets De La Digestion Gastrique Sur Les Propriétés Anthelminthiques De Zanthoxylum Zanthoxyloides (Lam.) Zepernick & Timlerto Et De Newbouldia Laevis (P.Beauv.) Sur Haemonchus Contortus." European Scientific Journal, ESJ 13, no. 24 (2017): 204. http://dx.doi.org/10.19044/esj.2017.v13n24p204.
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Several recent studies have shown that medicinal plants Zanthoxylum zanthoxyloides (Fagara) and Newbouldia laevis possess anthelmintic activities in vitro on different stages of development of gastrointestinal nematodes. The objective of this study was to evaluate the in vitro anthelmintic properties of residues from digestion in the rumen of leaf powders of both plants on the migration of the 3rd-stage larvae L3s of Haemonchus contortus. Residues obtained after incubation at 0 h, 24 h and 96 h kinetic points of the leaf powders of both plants in the rumen of sheep with fistulae were used for the assay. The larval migration inhibition test evaluated the anthelmintic properties of the methanolic extracts of residues of the two plants. The effect of plant extracts on larval migration was notdose- dependent (p> 0.05) but was a function of plant incubation time (p 0.05). N. Laevis seems to have retained his anthelmintic property after incubation in sacco in the rumen. On the other hand, Fagara seems to lose its effectiveness as it stays in the rumen. Findings obtained on these plants confirm their traditional use in veterinary medicine especially in the control of helminthiasis.
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Faramayuda, Fahrauk, Silvy Julian, Ari Sri Windyaswari, Totik Sri Mariani, Elfahmi ., and Sukrasno . "A COMPARATIVE PHARMACOGNOSTIC STUDY OF THE TWO Orthoshipon aristatus (BLUME) MIQ. VARIETIES." Journal of Experimental Biology and Agricultural Sciences 9, Spl-2-ICOPMES_2020 (2021): S228—S233. http://dx.doi.org/10.18006/2021.9(spl-2-icopmes_2020).s228.s233.
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The use of a plant as an ingredient in traditional medicine requires scientific evidence to determine its properties. Cat's whiskers (Orthosiphon aristatus) is one of the widely used traditional medicinal plants in various Asian and European countries. This study aimed to determine the pharmacognostic properties of purple and white varieties of O. aristatus. Aqueous and ethanolic extract of the stem and leaves of purple and white varieties of O. aristatus was prepared and investigated for the presence of active ingredients. The AlCl3 colorimetric method was used for the estimation of flavones and flavonols. The level of flavonoid was reported 13.06 ± 0.13 mg QE / 1g and 6.17 ± 0.049 mg QE / 1g for the leaves and stem extracts of purple varieties respectively while this value was reported 9.76 ± 0.15 mg QE / 1g and 3.79 ± 0.03 mg QE / 1g for the white variety. From the results of the study, it can be concluded that the purple variety has a significantly higher amount of flavonoid then the white variety.
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Opryshko, Maryna, Oleksandr Gyrenko, Lyudmyla Buyun, Halyna Tkachenko, Natalia Kurhaluk, and Vladimir Tomin. "IDENTIFICATION OF THE ANTIBACTERIAL EFFICACY OF ETHANOLIC EXTRACTS FROM AGLAONEMA COMMUTATUM SCHOTT LEAVES AND ITS CULTIVARS AGAINST ESCHERICHIA COLI STRAIN." Scientific and Technical Bulletin of the Institute of Animal Science NAAS of Ukraine, no. 123 (2020): 21–30. http://dx.doi.org/10.32900/2312-8402-2020-123-21-30.
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This study aimed to evaluate the antibacterial activity of ethanolic extracts obtained from the leaves of Aglaonema commutatum Schott and its cultivars («Malay Beauty», «Silver Queen», and «Silver King») against Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™) strain. The leaves of Aglaonema commutatum plants and its cultivars, cultivated under glasshouse conditions, were sampled at M. M. Gryshko National Botanic Garden (NBG), National Academy of Science of Ukraine (Kyiv, Ukraine). The leaves were brought into the laboratory for antimicrobial studies. Freshly sampled leaves were washed, weighed, and homogenized in 96% ethanol (in proportion 1:19) at room temperature. The extracts were then filtered and investigated for their antimicrobial activity. Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™) strain was used in our study. Antimicrobial activities of various ethanolic extracts obtained from leaves of Aglaonema commutatum plants and its cultivars («Malay Beauty», «Silver Queen», and «Silver King») against Escherichia coli (Migula) Castellani and Chalmers (ATCC® 25922™) strain was screened in the current study. The testing of the antibacterial activity of the plant extracts was carried out in vitro by the Kirby-Bauer disc diffusion technique. The leaf extracts from A. commutatum «Silver Queen» and A. commutatum 'Silver King' exhibited higher inhibitory activity than the extracts from A. commutatum and A. commutatum «Malay Beauty». Maximum in vitro inhibition was scored by A. commutatum «Silver Queen», followed by A. commutatum «Silver King», A. commutatum, and A. commutatum «Malay Beauty». In particular, the leaf extracts from A. commutatum «Silver Queen» and A. commutatum 'Silver King' exhibited higher inhibitory activity than the extracts from A. commutatum and A. commutatum «Malay Beauty». Maximum in vitro inhibition was scored by A. commutatum «Silver Queen», followed by A. commutatum «Silver King», A. commutatum, and A. commutatum «Malay Beauty», which presented inhibition zones of (18.6±1.2) mm, (16.1±0.9) mm, (15.7±1.1) mm, and (13.5±1.0) mm, respectively. In the case of the positive controls, 96% ethanol possesses a mild anti-E. coli effect, which presented inhibition zones of (9.5±1.2) mm. The inhibition zone diameters were increased by 96% (p<0.05) for A. commutatum «Silver Queen», by 69 % (p<0.05) for A. commutatum «Silver King», by 65 % (p<0.05) for A. commutatum, and by 42 % (p<0.05) for A. commutatum «Malay Beauty». Thus, the use of these plants in traditional medicine and veterinary medicine was experimentally confirmed as a potential source of raw materials for the development of medicines in the future, as well as for the development of innovative feed for farm animals.
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Bizimenyera, S. E., G. E. Swan, H. Chikoto, and J. N. Eloff. "Rationale for using Peltophorum africanum (Fabaceae) extracts in veterinary medicine." Journal of the South African Veterinary Association 76, no. 2 (2005). http://dx.doi.org/10.4102/jsava.v76i2.397.
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Peltophorum africanum (Fabaceae) is a deciduous tree widespread in southern Africa. The plant has many ethnomedical and ethnoveterinary uses. Root and bark decoctions are used to treat diarrhoea, dysentery, sore throat, wounds, back and joint pains, HIV-AIDS, venereal diseases and infertility. Pastoralists and rural farmers use the root and bark extracts to treat diarrhoea, dysentery, infertility, and to promote well-being and resistance to diseases in cattle. To evaluate these ethnobotanical leads, dried leaves, stem bark and root bark were extracted with ethanol, acetone, dichloromethane and hexane. Polyphenols in the extract were determined by the Folin-Ciocalteu method with gallic acid as standard. Qualitative antioxidant activity was screened by spraying thin layer chromatograms (TLC) of the extracts with 0.2 % 1, 1-diphenyl-2-picryl hydrazyl (DPPH), and quantified with Trolox equivalent antioxidant capacity (TEAC) assay. Minimum inhibitory concentration (MIC) and total antibacterial activity (TAA) were determined by serial microplate dilution for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Enterococcus faecalis, with gentamicin as standard and tetrazolium violet as growth indicator. Acetone and ethanol extracted the largest quantity of material. Polyphenols concentration was 49.2% in acetone extract of the root and 3.8 % in dichloromethane extract of the leaf. Antioxidant activity of at least 5 antioxidant compounds as measured by TEAC ranged from 1.34 (ethanol extract of the root) to 0.01 (hexane extract of the leaf). The total antibacterial activity (volume to which active compounds present in 1 g plant material can be diluted and still inhibit bacterial growth) was 1263 mℓ/g for ethanol extract of the root against S. aureus, and 800 mℓ/g for acetone extract of the root against P. aeruginosa. There was substantial activity against both Gram-positive and Gram-negative bacteria, with MIC values of 0.08 mg/mℓ for S. aureus and 0.16 mg/mℓ for P. aeruginosa. There is therefore a rationale for the traditional use of root and bark of P. africanum in treating bacterial infection related diseases.
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Nyahangare, Emmanuel T., Brighton M. Mvumi, Lyndy J. McGaw, and Jacobus N. Eloff. "Addition of a surfactant to water increases the acaricidal activity of extracts of some plant species used to control ticks by Zimbabwean smallholder farmers." BMC Veterinary Research 15, no. 1 (2019). http://dx.doi.org/10.1186/s12917-019-2078-3.
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Abstract Background Many studies have revealed that bioactive compounds for different indications are not extracted from plants with water, the only extractant practically available to rural communities. We compared the acaricidal activity of acetone extracts of 13 species used traditionally to protect cattle against ticks. We also investigated if the extraction of biologically active compounds against Rhipicephalus (Boophilus) decoloratus ticks could be enhanced by adding a liquid soap that is locally available to smallholder farmers. Methods A total of 13 plant species selected based on reported traditional use in Zimbabwe, were dried and finely ground before extraction with water, or water plus a surfactant, or acetone. The adapted Shaw Larval Immersion Test (SLIT) method was used to determine the activity of acetone and crude water extracts with or without liquid soap against the tick larvae. The activity of four fractions of crude acetone extracts (extracted using solvents of different polarity), of the most active plant species, Maerua edulis (tuber and leaf) was also compared to identify the most active fraction. Results Aqueous plant extracts were not toxic to ticks, but the addition of 1% liquid soap as a surfactant increased mortality of the R. (B) decoloratus larvae significantly. With the Maerua edulis tuber extract, the efficacy of the 1% liquid soap was comparable to that of the amitraz based commercial synthetic acaricide. The use of acetone as an extractant, also increased the mortality of the tick larvae in all the plant species. With M. edulis (tuber and leaf), Monadenium lugardae and Kleinia sp. acetone extracts, the activity was comparable to that of the positive control (a commercially available amitraz-based synthetic acaricide). The non-polar fractions of the acetone extract of leaf and tuber of M. edulis caused up to 100% mortality. This indicates that non-polar to intermediate polarity compounds are responsible for the acaricidal activity. Conclusion Organic solvents such as acetone extracted active compounds but water did not. By adding commonly available dishwashing soap to water active compounds were extracted leading to a high acaricidal activity of the plant extracts. In some cases, it was as active as non-polar extracts and a synthetic commercial acaricide (positive control). This approach makes it possible for the smallholder farmers and traditional healers to extract biologically active compounds from plants by using water.
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Widhiantara, I. Gede, and I. Made Jawi. "Phytochemical composition and health properties of Sembung plant (Blumea balsamifera): A review." Veterinary World, May 17, 2021, 1185–96. http://dx.doi.org/10.14202/vetworld.2021.1185-1196.
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Indonesia's mindset has been focusing on the use of natural medicines, food, and healing practices widely recognized by the nation's culture. Traditional medicines and herbs used in traditional medicine can often lead to the discovery of drugs against certain diseases. The aim of this review was to study evidence-based data on the importance of Sembung plant, Blumea balsamifera, as a potential traditional medicine. The distribution, ethnopharmacology, secondary metabolites, and bioactivity against several diseases are focused in this review. Information and research related to Sembung plant were searched using the terms "B. balsamifera," "phytochemicals," and "pharmacological activity" on ResearchGate, Google Scholar, Science Direct, PubMed, and scientific information-based databases up to 2020. Several ethnomedical articles recommend B. balsamifera for the treatment of sinusitis, colic pain, cough, kidney stones, flu, or as a diuretic. This knowledge has already been applied in several countries in Southeast Asia. B. balsamifera has been reported to contain several phytochemicals both volatile (terpenoids, fatty acids, phenols, alcohol, aldehydes, ethers, ketones, pyridines, furans, and alkanes) and non-volatile (flavonoids, flavanones, and chalcones). Extracts and phytochemicals of B. balsamifera contain several biological capacities such as antioxidant, antimicrobial, antifungal, anti-inflammatory, hypolipidemic, anti-infertility, hepatoprotective activity, antidiabetic, gastroprotective, antitumor, anticancer, and immunomodulatory agent against Coronavirus disease-19 infection. This review provides essential data for the potential application of B. balsamifera as a nutraceutical or in future medicinal preparations.
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Roy, Saptarshi, and Larisha Mawkhlieng Lyndem. "An in vitro confirmation of the ethonopharmacological use of Senna plants as anthelmintic against rumen fluke Paramphistomum gracile." BMC Veterinary Research 15, no. 1 (2019). http://dx.doi.org/10.1186/s12917-019-2094-3.
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Abstract Background Paramphistomosis is a pathogenic disease of domesticated ruminants, causing great economic loss in dairy industry and meat production. It is considered as a neglected tropical disease with highest prevalence throughout tropical and subtropical regions, particularly in Africa, Asia, Europe, and Australia. There are few trematocidal drugs available in the market. Most are resistant and have elevated side effects. Therefore, alternative trematocidal drugs need to discover. This study was conducted to evaluate three plants leaf extracts (from Senna alata, S. alexandrina, and S. occidentalis) as effective remedies against gastrointestinal trematode parasite (Paramphistomum gracile) of ruminants. Live adult parasites were collected in 0.1 M phosphate-buffered saline (PBS) from fresh autopsied goat’s rumen. Parasites were incubated in leaf extracts of S. alata, S. alexandrina and, S. occidentalis individually and in combination (1:1) ratio at 37 ± 1°C. Treatment media contain extracts at different concentrations (10, 20 and 40 mg/mL) in 10 mL of 0.1 M PBS with 1% dimethylsulphoxide (DMSO). Parasites in control group were incubated in PBS without extract. The efficacy of three Senna extracts was evaluated on the basis of dose-dependent motility and mortality of the trematode. Immediately after paralysis, all treated parasites were collected for histology, SEM and biochemical study. Results Dose-dependent efficacy was observed in terms of motility and time of mortality in all treated parasites after exposure in various concentrations of the Senna plant extracts. S. occidentalis and S. alexandrina showed better efficacy in combination than comparing with individual treatment groups. Histological study and scanning electron microscopic observations revealed conspicuous deformity of surface architecture in all treated parasites. Scanning electron micrographs also revealed shrinkage, vacuolization, infoldings and blebbings on the body surface of treated worms. Activities of tegumental enzymes were inhibited in all treatment groups compared to control. Conclusion The overall findings from this study revealed that all three Senna leaf extracts individually and in combination showed potential antitrematocidal activity against Paramphistomum gracile by damaging body tegument and neural propagation. Thus, this study confirmed that all three Senna extracts can be considered as a potential drug-like candidate in indigenous system of traditional medicine against trematode infections in livestock. Graphical abstract
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THANH VAN NGUYEN and HAI THANH NGUYEN. "STUDY ON ANTIBACTERIAL EFFECTS OF SEVERAL VIETNAMESE MEDICINE PLANTS AND THEIR RELATIONSHIPS WITH POLYPHENOL CONTENTS." Asian Journal of Pharmaceutical and Clinical Research, March 15, 2019, 257–65. http://dx.doi.org/10.22159/ajpcr.2019.v12i4.32290.
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Objective: Our study evaluated the inhibitory effects of 13 plant materials on bacterial growth, so as to explain their traditional uses in bacterial infections. In addition, the correlation between antibacterial effects of plants and the polyphenol contents or antioxidant activities was also verified to investigate if these properties were responsible for the plant antibacterial effects.
 Methods: We tested extracts with 20 bacterial strains purchased from Japan Veterinary Products Association (JVPA, Tokyo, Japan) and also the Escherichia coli (E. coli) and Streptococcus suis (S. suis) strains that we had previously isolated from livestock. After polyphenol contents and antioxidant activities were measured, we examined their correlations with antibacterial effects.
 Results: The results showed that all extracts exerted inhibitory effects on tested bacteria and at different levels. Among all of samples, betel and mother rhizome of turmeric showed the strongest effects and also exerted inhibition on antibiotic-resistant E. coli and S. suis bacteria. In addition, direct and significant correlations between antibacterial effects’ and polyphenol content’s values were established and thus demonstrating that antibacterial effects of plants were attributable, at least in part, to the presence of polyphenol compounds.
 Conclusions: Our study demonstrated the antibacterial effects of 13 plant materials and, therefore, partly gives pharmacological basis to explain their traditional uses in diseases associated with bacterial infections. In addition, as betel and mother rhizome of turmeric showed the highest effects, we suggested that they might serve as the most promissory candidates for bacterial treatment. However, follow-up researches are still required to assess their potentials.
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Maqbool, Shahid, Najeeb Ullah, Aqal Zaman, et al. "Phytochemical Screening, In-vitro and In-vivo Anti-diabetic Activity of Nelumbo nucifera Leaves against Alloxan-Induced Diabetic Rabbits." Indian Journal of Animal Research, of (December 30, 2019). http://dx.doi.org/10.18805/ijar.b-1181.
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A recent survey has demonstrated that traditional healers and herbalists frequently use Nelumbo nucifera plant to treat diabetes. The plant investigated here has been previously reported as natural folk medicine, but until now no scientific investigation of potential anti-diabetic effects have been reported. Plant leaves of Nelumbo nucifera were screened out for quantitative analysis of phytochemicals, in-vitro anti-diabetic activity (Alpha-amylase inhibitory assay) and In-vivo anti-diabetic assay of animals (rabbits). Quantitative analysis indicates that different phytochemicals were present in plant leaves extract/fractions. In-vitro antidiabetic analysis results poses that butanol fraction is significantly more active than other fractions or extract in antihyperglycemic activity with minimum IC50 value of 8.56µg/ml. For In-vivo anti-diabetic assay, animals (rabbits) were examined and animals were divided into 10 groups and except blank group all were treated with standard and plant extracts/fractions doses (25mg and 50mg per Kg body weight) as an aqueous suspension. Their blood sugar level was thoroughly recorded with a glucometer before the administration of sample and standard drug. Alloxan Monohydrate was administrated orally (as an ice-cold water solution) at dose rate 150mg/kg body weight for one week to make animals hyperglycemic. In-vivo analysis indicates that butanol fraction is the most active fraction in antihyperglycemic activity. So Leaves of N. nucifera can be used as antidiabetic remedy. So, it is strongly recommended that N. nucifera leaves should be elucidated further for isolation of active phytochemicals.
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Saheed, Yunusa, Mustapha Usman Nasir, Baballe Abbas, and Rabi Yakubu Bello. "GC-MS Analysis and Antimicrobial Spectrum of Stem Bark Extracts of Ficus sycomorus." Microbiology Research Journal International, October 28, 2020, 118–28. http://dx.doi.org/10.9734/mrji/2020/v30i830257.
Full textAbstract:
Aim: This work investigated the gas chromatography-mass spectroscopy of methanolic extract and antimicrobial spectrum of acetone and aqueous crude extracts of Ficus sycomorus stem bark.
 Place and Duration of the Study: Department chemistry research laboratory and microbiology laboratory, Yobe State University, Damaturu between April 2019 and August, 2019.
 Methodology: Pure isolates of Staphylococcus aureus, Salmonella typhi, Shigella dysentariae, proteus spp, Pseudomonas auruginosa, Escherichia coli, Bacillus subtilis, Klebsiella pneumonia and a fungi, Candida albicans were collected from National Veterinary Research Institute (NVRI), Vom, Plateau State. The plant was collected and the extract was used in preliminary phytochemical screening, GC-MS analysis and reconstituted for antimicrobial activities testing.
 Results: The preliminary phytochemical screening revealed presence of saponin, flavonoids, alkaloids, carbohydrates, cardiac glycoside and tannin. GC-MS analysis revealed the presence of heterocyclic members of pyrazole, imidazole, triazole, thiazole and 1, 3-Benzenedicarboxylic acid, bis (2-hethylhexyl) ester. Acetone extract showed the greatest antimicrobial activity against Bacillus subtilis, Pseudomonas aeruginosa, Proteus mirabilis and Shigella spp while the aqueous extract produced antibacterial activity against Bacillus subtilis, Staphylococcus aureus, Klebsiella pneumonia and Candida albicans. Both acetone and aqueous extracts of both stem bark extracts did not exhibit any activity against Escherichia coli. All the extracts were tested at 100mg/ml concentration. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) (125 – 250 mg/ml) of both extracts were higher than those of ciprofloxacin (5.0 mg/ml).
 Conclusion: There is scientific basis for the uses of F. sycomorus in traditional medicine for the treatment of various diseases as prescribed earlier on and stronger basis for recommendation for its cautious use for the presence of 2-ethylhexyl which could be Carcinogenic. This is first study to identify 2-ethylhexyl from F. sycomorus stem bark extract and recommend cautious use.
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Sonter, Shruti, Shringika Mishra, Manish Kumar Dwivedi, and Prashant Kumar Singh. "Chemical profiling, in vitro antioxidant, membrane stabilizing and antimicrobial properties of wild growing Murraya paniculata from Amarkantak (M.P.)." Scientific Reports 11, no. 1 (2021). http://dx.doi.org/10.1038/s41598-021-87404-7.
Full textAbstract:
AbstractThe excessive usage of antibiotics in humans and veterinary medicine has lead to the emergence of antibiotic resistance and now requires the use of novel antibiotics. There has been increased interest towards plants as source of drugs because of their pharmacological potency and long traditional usage. The aim of the current study was to evaluate bioactive components, antioxidant, and anti-inflammatory activities of the leaf extracts of Murraya paniculata, a plant traditionally used in Indian medicinal system. Evaluations were made for phytochemical analysis, antioxidant, membrane stabilizing, and antimicrobial activities. The methanol extract displayed the highest flavonoid and phenolic content, the acetone extract demonstrated considerable ABTS inhibitory activity (IC50value:555.18 ± 1.68 µg/mL) and the hexane extract exhibited highest H2O2 radical scavenging activity (IC50value: 509.84 ± 3.03 µg/mL). The aqueous extract displayed 19.4 ± 0.66% RBC hemolysis and 80.5 ± 0.66% protection caused by hypotonic solution at high concentration of the extract. The fractions of hexane extract revealed a higher zone of inhibition than crude extract. The major components found in the fractions were cyclohexane (40.11%) and 3-(6-Methoxy-3-methyl-2-benzofuranyl) Cyclohexanone (13.68%) as analyzed by GC–MS/MS technique. The current results validate the traditional use of the M. paniculata and warrant its potential in drug development programs in further investigations.
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KALABA, Vesna, Željko SLADOJEVIĆ, Željka MARJANOVIĆ BALABAN, Dragana KALABA, and Ivona PANIĆ. "ANTIBACTERIAL PROPERTIES OF WHITE LILY (Lilium candidum) EXTRACT." ВЕТЕРИНАРСКИ ЖУРНАЛ РЕПУБЛИКЕ СРПСКЕ 19, no. 2 (2020). http://dx.doi.org/10.7251/vetjen1902274k.
Full textAbstract:
Natural extracts made from fresh plant material and biologically active compounds isolated from different plant species that have been used in folk medicine for centuries, may present valuable sources for production of novel natural preservatives and medicinal preparations. White lily-Lilium candidum is also used, for a long period of time, in folk medicine for healing of burns and frostbites, otitis and rhinitis as well as balm for washing wounds and cuts. Roots, leaves and flowers have medical properties. Nevertheless, there is little information in literature related to antibacterial effect of white lily extract on certain pathogens and there is a need to determine the justification for its use in traditional medicine. Antibacterial activity of White lily extract on five reference cultures (Escherichia coli WDCM 00090, Listeria monocytogenes WDCM 00020, Salmonella enterica WDCM 00030, Pseudomonas aeruginosa WDCM 00024) and seven bacterial cultures (Escherichia coli, Providencia stuartii, Pseudomonas spp. ß-hemolytic Escherichia coli, coagulase positive staphylococcus, Staphylococcus aureus, group D Sreptococcus) from Laboratory for Microbiology, Public Veterinary Institute of Republic of Srpska "Dr. Vaso Butozan", Banja Luka, were examined in this study. Results confirmed that white lily extract show certain antibacterial activity against examined pathogens used in this study. Antibacterial activity of White lily extract was in the range from 5.33 mm to 18.88 mm depending of bacterial strain and concentration of white lily extract.