Journal articles: 'Plant histology'

1

Freeman, H. E. "Plant histology: clearing and the optical section." Journal of Biological Education 19, no. 1 (March 1985): 13–15. http://dx.doi.org/10.1080/00219266.1985.9654676.

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Lin, C. T., D. Sun, G. X. Song, and J. Y. Wu. "Calmodulin: localization in plant tissues." Journal of Histochemistry & Cytochemistry 34, no. 5 (May 1986): 561–67. http://dx.doi.org/10.1177/34.5.3084624.

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Calmodulin was purified from bovine brain by preparative SDS-polyacrylamide gel electrophoresis. The denatured, purified calmodulin was used to immunize rabbits to produce antiserum. This antiserum was used to study the distribution of calmodulin in plant tissues by indirect immunohistochemistry. The root tips from corn seeds, oat seeds, peanuts, spaghetti squash seeds, and the terminal buds of spinach were investigated. A method for plant tissue sectioning and inhibition of endogenous peroxide activity was developed. In the corn root section, reaction product from anti-calmodulin was found mainly in the root cap cells. Lesser but significant amounts of calmodulin were localized in metaxylem elements, in some stele cells surrounding metaxylem elements, in apical initials, and in the cortical cells. Similar findings were also observed in other root tips from oat seeds, peanuts, and spaghetti squash seeds. In the terminal buds of the spinach, calmodulin-stained cells were highly concentrated in the apical meristem and leaf primordium. These findings suggest that the high concentration of calmodulin in the root cap may be important in relation to gravitropism and growth development.

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Cloney, R. A. "Histology: An Interactive Virtual Microscope." Integrative and Comparative Biology 43, no. 2 (April 1, 2003): 360. http://dx.doi.org/10.1093/icb/43.2.360.

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Höglund, Anna-Stina, Alan M. Jones, and Lars-Göran Josefsson. "An Antigen Expressed During Plant Vascular Development Crossreacts with Antibodies Towards KLH (Keyhole Limpet Hemocyanin)." Journal of Histochemistry & Cytochemistry 50, no. 8 (August 2002): 999–1003. http://dx.doi.org/10.1177/002215540205000801.

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An antigen present in plant vascular tissue crossreacts with antibodies towards keyhole limpet hemocyanin (KLH). The antigen is present in xylem and vascular cambium, as evidenced by immunocytochemical staining of plant sections. This cell type assignment was confirmed by staining of mesophyll cell cultures from Zinnia elegans L. undergoing tracheary cell differentiation. The strongest staining both in sections and cell cultures occured in cells and tissues during early stages of differentiation. Although the anti-KLH antibodies can easily be removed by affinity purification, our findings suggest that a certain caution is needed when KLH is used as an immunological carrier for studies in plants.

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Luthfi, Muhammad Ja’far. "Effect of Lunasia amara Blanco on Sperm Number, Sperm Motility, and Testicular Histology of Male Rats." Biology, Medicine, & Natural Product Chemistry 4, no. 2 (October 15, 2015): 31. http://dx.doi.org/10.14421/biomedich.2015.42.31-33.

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<p>Sanrego (<em>Lunasia amara</em>), has been used in the folk medicine to increase and/or to treat male fertility. However there is no scientific evidence to confirm the positive effect of the plant on an improvement of male fertility. The objective of this research was to study the effects of the plant (on adult Sprague-Dawley male rats) at the doses of 30 mg/kg, 60 mg/kg, and 90 mg/kg on the sperm count, motility, and testicular histology. Administration were given by force-feeding between 10.00 am and 12.00 pm daily for a period of 42 days followed by sperm quality analysis and testicular histology evaluation. The sperm analysis showed that the sanrego increased the sperm count and sperm motility. The testicular histology also revealed positive effect of the plant on spermatogenesis. Overall the present study showed the sanrego is potential plant to increase male fertility.</p>

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Laliberté, Sylvie, and Maurice Lalonde. "Histology and ultrastructure of caulogenic callus cultures of Larix ×eurolepis." Canadian Journal of Botany 68, no. 5 (May 1, 1990): 979–89. http://dx.doi.org/10.1139/b90-124.

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Caulogenic callus cultures intiated from vegetative short shoot buds of a mature Larix ×eurolepis Henry (hybrid larch) were studied by light and electron microscopy. Callus tissue showed a zonation in pigmentation, as color varied from green to tan and brown. Green areas displayed characteristics of actively growing tissues, contained numerous chloroplasts and starch granules, and showed a range in the amount of vacuolar tannin deposits. Vascular clusters were present in callus tissue associated with adventitious shoots, which were well vascularized. Tan and brown areas had massive amounts of starch granules, tannin deposits, and sclereids. Numerous cells in brown areas were in a senescent state. Browning of tissues increased with time during each subculture and was concomitant with an increase in shoot productivity. Extracellular substances were apparently extruded from primary cell walls in tan and brown areas. Key words: caulogenic calli, conifer, histology, Larix, tissue culture, ultrastructure.

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Kozub, N. O., L. A. Pilipenko, I. O. Sozinov, Ya B. Blume, and O. O. Sozinov. "Genetically modified plants and plant protection problems: Progress and estimation of potential risks." Cytology and Genetics 46, no. 4 (July 2012): 251–62. http://dx.doi.org/10.3103/s0095452712040081.

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ROBINSON, DAVID G. "Plant Golgi ultrastructure." Journal of Microscopy 280, no. 2 (May 27, 2020): 111–21. http://dx.doi.org/10.1111/jmi.12899.

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SHERWINCARLQUIST, F., and D. GOWANS. "Secondary growth and wood histology of." Botanical Journal of the Linnean Society 118, no. 2 (June 1995): 107–21. http://dx.doi.org/10.1016/s0024-4074(95)80011-5.

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Swiecki, T. J., and J. D. MacDonald. "Histology of chrysanthemum roots exposed to salinity stress and Phytophthora cryptogea." Canadian Journal of Botany 66, no. 2 (February 1, 1988): 280–88. http://dx.doi.org/10.1139/b88-046.

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Rooted cuttings of Chrysanthemum morifolium 'Paragon' grown in nutrient solution were given a 24-h pulse exposure to salinity by amending the solution with 200 mequiv. NaCl/L. The cuttings were then returned to nonsalinized solution; one half were inoculated with motile zoospores of Phytophthora cryptogea Pethybr. & Laff, and the other half were maintained as non-inoculated controls. Nonstressed cuttings also were inoculated with P. cryptogea, and roots from all treatments were sampled at various intervals thereafter for light and electron microscopy. Penetration of nonstressed roots by P. cryptogea was frequently limited to three or four cell layers 6–12 h after inoculation. Formation of appositions adjacent to hyphae, increased wall staining density, and accumulation of osmiophilic material in cell vacuoles were associated with sites of limited penetration. In contrast, hyphae of P. cryptogea rapidly colonized salinity-stressed roots, causing extensive necrosis within 12–24 h after inoculation. The cytological responses to infection seen in nonstressed roots were rarely observed in stressed roots, indicating that salinity stress inhibits active root defense mechanisms.

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Itabashi, Tetsuo, Shinso Yokota, and Nobuo Yoshizawa. "The Seasonal Occurrence and Histology of Septate Fibers in Kalopanax Pictus." IAWA Journal 20, no. 4 (1999): 395–404. http://dx.doi.org/10.1163/22941932-90001564.

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Septate wood fibers were abundant in the following parts of growth rings of Kalopanax pictus Nakai: 1) around the vessels, 2) in the vicinity of ray cells, 3) in terminal regions of the growth rings. Septum formation in wood fibers progressed from the initial region (pore zone) towards the terminal region within a current growth ring with progressing 1ignification of the wood fiber walls. Many septate wood fibers at the end of the growth ring had radially continuous septa. Karyokinesis was observed in severa1 wood fibers before the initiation of septum formation, while lignification was in progress after the completion of the S3 layer deposition. This suggests that the septation starts in parallel with the progress of lignification after the deposition of the S3 layer.

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Sivolap, Yu M. "Molecular markers and plant breeding." Cytology and Genetics 47, no. 3 (May 2013): 188–95. http://dx.doi.org/10.3103/s0095452713030080.

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Motte, P. M., R. Loppes, M. Menager, and R. Deltour. "Three-dimensional electron microscopy of ribosomal chromatin in two higher plants: a cytochemical, immunocytochemical, and in situ hybridization approach." Journal of Histochemistry & Cytochemistry 39, no. 11 (November 1991): 1495–506. http://dx.doi.org/10.1177/39.11.1918926.

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We report the 3-D arrangement of DNA within the nucleolar subcomponents from two evolutionary distant higher plants, Zea mays and Sinapis alba. These species are particularly convenient to study the spatial organization of plant intranucleolar DNA, since their nucleoli have been previously reconstructed in 3-D from serial ultra-thin sections. We used the osmium ammine-B complex (a specific DNA stain) on thick sections of Lowicryl-embedded root fragments. Immunocytochemical techniques using anti-DNA antibodies and rDNA/rDNA in situ hybridization were also applied on ultra-thin sections. We showed on tilted images that the OA-B stains DNA throughout the whole thickness of the section. In addition, very low quantities of cytoplasmic DNA were stained by this complex, which is now the best DNA stain used in electron microscopy. Within the nucleoli the DNA was localized in the fibrillar centers, where large clumps of dense chromatin were also visible. In the two plant species intranucleolar chromatin forms a complex network with strands partially linked to chromosomal nucleolar-organizing regions identified by in situ hybridization. This study describes for the first time the spatial arrangement of the intranucleolar chromatin in nucleoli of higher plants using high-resolution techniques.

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McCartney, Lesley, Susan E. Marcus, and J. Paul Knox. "Monoclonal Antibodies to Plant Cell Wall Xylans and Arabinoxylans." Journal of Histochemistry & Cytochemistry 53, no. 4 (April 2005): 543–46. http://dx.doi.org/10.1369/jhc.4b6578.2005.

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Two rat monoclonal antibodies have been generated to plant cell wall (1→4)-β-D-xylans using a penta-1,4-xylanoside-containing neoglycoprotein as an immunogen. The monoclonal antibodies, designated LM10 and LM11, have different specificities to xylans in relation to the substitution of the xylan backbone as indicated by immunodot assays and competitive-inhibition ELISAs. LM10 is specific to unsubstituted or low-substituted xylans, whereas LM11 binds to wheat arabinoxylan in addition to unsubstituted xylans. Immunocytochemical analyses indicated the presence of both epitopes in secondary cell walls of xylem but differences in occurrence in other cell types.

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Guillemin, Florence, Marie-Françoise Devaux, and Fabienne Guillon. "EVALUATION OF PLANT HISTOLOGY BY AUTOMATIC CLUSTERING BASED ON INDIVIDUAL CELL MORPHOLOGICAL FEATURES." Image Analysis & Stereology 23, no. 1 (May 3, 2011): 13. http://dx.doi.org/10.5566/ias.v23.p13-22.

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A procedure has been developed for the automatic clustering of plant cells observed by confocal microscopy. The contribution of cell morphological features to reveal histological regions has been investigated. Several adjacent images were acquired to visualise a representative region of the sample and a mosaic image was built. The cell size and shape and the cell wall thickness were quantified. The extracted features were used to automatically classify the cells into morphological groups. The technique made it possible to split the cell population into 8 groups mainly corresponding to histological regions of beet root.

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Najib, Nurmadina, Tyas Rini Saraswati, and Sunarno Sunarno. "Rat Lung Histology After Exposure to Cigarette Smooke Made from Brugmansia suaveolens Plant." International Journal of Medical, Pharmacy and Drug Research 4, no. 6 (2020): 44–49. http://dx.doi.org/10.22161/ijmpd.4.6.2.

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Novikov, Andriy, and Mariia Sup-Novikova. "Modified staining protocol with Safranin O and Astra Blue for the plant histology." Plant Introduction 89-90 (June 16, 2021): 110–13. http://dx.doi.org/10.46341/pi2021005.

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Many staining protocols are widely applied in botanical microtechniques and serve specific histological purposes. In particular, some dyes are used simultaneously to receive contrasting colorations of different chemical structures, e.g., lignin and cellulose. One of the most popular differential staining protocols is based on the Safranin O / Astra Blue dyes combination. Safranin O is a water-soluble basic dye that stains lignin in red. Astra Blue is also a water-soluble dye but having an acidic reaction, which stains cellulose in blue. Usually, a 1–2 % solution of Safranin O in distilled water or 50–70 % ethanol is applied in combination with the 0.5–1 % water solution of Astra Blue to detect lignified structures and obtain contrasting pictures convenient for the light microscopy. For a long time, Astra Blue was used exclusively with water solutions, and such recommendation without additional options is indicated on producers’ web sites. However, in 2002 it was proposed to use 1 % Astra Blue solution in 95 % ethanol to identify the lignified tissues. Later, such an ethanol solution of Astra Blue was also successfully applied by other researchers for different experimental purposes.We tested the modified staining protocol with the application of both 1 % Safranin O and Astra Blue solutions in a slightly lower concentration of ethanol (70 %) on the flower buds of Gagea lutea (Liliaceae) and found it working well. We believe that such a modified protocol with the solutions of these two dyes in 70 % ethanol allows simplifying the procedure of the plant material staining due to application of the same concentrations of dissolvent and reducing the difference in solvent concentration between two following contrasting staining solutions. Such differential staining can be effectively applied for plant histology purposes, especially where there is a need to distinguish lignified structures and secretory tissues.

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Westermeier, Anna S., Natalie Hiss, Thomas Speck, and Simon Poppinga. "Functional–morphological analyses of the delicate snap-traps of the aquatic carnivorous waterwheel plant (Aldrovanda vesiculosa) with 2D and 3D imaging techniques." Annals of Botany 126, no. 6 (August 11, 2020): 1099–107. http://dx.doi.org/10.1093/aob/mcaa135.

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Abstract Background and Aims The endangered aquatic carnivorous waterwheel plant (Aldrovanda vesiculosa) catches prey with 3–5-mm-long underwater snap-traps. Trapping lasts 10–20 ms, which is 10-fold faster than in its famous sister, the terrestrial Venus flytrap (Dionaea muscipula). After successful capture, the trap narrows further and forms a ‘stomach’ for the digestion of prey, the so-called ‘sickle-shaped cavity’. To date, knowledge is very scarce regarding the deformation process during narrowing and consequent functional morphology of the trap. Methods We performed comparative analyses of virtual 3D histology using computed tomography (CT) and conventional 2D histology. For 3D histology we established a contrasting agent-based preparation protocol tailored for delicate underwater plant tissues. Key Results Our analyses reveal new structural insights into the adaptive architecture of the complex A. vesiculosa snap-trap. In particular, we discuss in detail the arrangement of sensitive trigger hairs inside the trap and present actual 3D representations of traps with prey. In addition, we provide trap volume calculations at different narrowing stages. Furthermore, the motile zone close to the trap midrib, which is thought to promote not only the fast trap closure by hydraulics but also the subsequent trap narrowing and trap reopening, is described and discussed for the first time in its entirety. Conclusions Our research contributes to the understanding of a complex, fast and reversible underwater plant movement and supplements preparation protocols for CT analyses of other non-lignified and sensitive plant structures.

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SHERWIN, CARLQUIST, and DAVID A. GOWANS. "Secondary growth and wood histology of Welwitschia." Botanical Journal of the Linnean Society 118, no. 2 (June 1995): 107–21. http://dx.doi.org/10.1111/j.1095-8339.1995.tb00464.x.

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Santana, Marconi Bonfim de, Antonio Diego Brandão Melo, Daniel Ribeiro Cruz, Cesar Augusto Pospissil Garbossa, Carla de Andrade, Vinicius de Souza Cantarelli, and Leandro Batista Costa. "Alternatives to antibiotic growth promoters for weanling pigs." Ciência Rural 45, no. 6 (June 2015): 1093–98. http://dx.doi.org/10.1590/0103-8478cr20140407.

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An experiment was conducted to evaluate the addition of sodium butyrate, plant extracts and nucleotides on weanling pig performance, digestive content pH, organ morphometry, and intestinal epithelial histology. A total of 90 piglets at 21 days of age and an average initial weight of 6.35±0.34kg were used. The piglets were distributed in a randomized complete block design with five treatments, six replicates, and three animals per experimental unit. The treatments consisted of the following: Control: basal diet without antibiotic; Antibiotic: basal diet with 40mg kg-1 colistin sulfate and Additive: 1000, 1500 and 2000mg kg-1 of a combination of sodium butyrate + plant extracts + nucleotides. The experiment lasted 35 days, at which time one animal was slaughtered to assess pH of the digestive contents, morphometry of the organs and histology of the intestinal epithelium. No differences were found (P>0.05) in the performance, pH of the digestive contents, morphometry of the organs or histology of the intestinal epithelium by the analysis of orthogonal polynomials or contrasts. The combination of sodium butyrate, plant extracts and nucleotides not improved the productive characteristics of weanling pigs.

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Moreno, F. J., R. M. Rodrigo, and G. Garcia-Herdugo. "Ag-NOR proteins and rDNA transcriptional activity in plant cells." Journal of Histochemistry & Cytochemistry 38, no. 12 (December 1990): 1879–87. http://dx.doi.org/10.1177/38.12.1701461.

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In this work we used Allium cepa root meristem cells in actively growing conditions and under treatment with the protein synthesis inhibitors cycloheximide (CHM) and puromycin. Morphological and quantitative results indicate that these drugs induce dramatic alterations in nucleolar structure reflected by a decrease of nucleolar size, much more evident under treatment with CHM, and by segregation of its main components. Quantitative analysis shows a decrease in NOR-silver staining after treatment with CHM, whereas in cells treated with puromycin NOR-silver staining remains constant. Our results reveal a decrease in the Ag-NOR proteins under conditions of diminished cell activity, suggesting a direct relationship between the quantity of Ag-NOR proteins and transcriptional activity. Using two-dimensional gel electrophoresis and NOR-silver staining in gels, we have characterized some proteins corresponding to molecular weights of 28 and 31 KD and pI of approximately 5.2. After treatment with CHM, reactivity of these proteins against NOR-silver staining is diminished. By means of a morphological study, analysis of NOR-silver staining, and of anti-DNA and RNAse-gold labeling, we have tried an approach to the nucleolar organization in plant cells. Our results suggest that the fibrillar component shows a reticular distribution where fibrillar centers, as described in animal cells, are not distinguished.

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Joye, Gary F., and Rex N. Paul. "Histology of Infection of Hydrilla verticillata by Macrophomina phaseolina." Weed Science 40, no. 2 (June 1992): 288–95. http://dx.doi.org/10.1017/s0043174500057362.

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Infection of Hydrilla verticillata by Macrophomina phaseolina was investigated using scanning and transmission electron microscopy. Sprigs of plants in petri plates were inoculated with suspensions of fungal hyphae. Samples of inoculated and noninoculated plants were taken over time. Fungal cells attached to lower epidermal cell walls but not the upper epidermal cell walls of leaves. In less than 40 h, penetration through the cell wall was completed and colonization of host cells was observed. Penetration of upper epidermis was limited to the cell wall adjacent to a lower epidermal cell. No penetration was observed through the outer cell wall of upper epidermis. Inhibition of penetration through the outer cell wall of the upper epidermis may be attributable to an osmiophilic layer below the cell wall. Disruption of the host cell walls and subsequent host cell death was preceded by massive colonization of the host by this pathogen.

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Budimir, S. "Developmental Histology of Organogenic and Embryogenic Tissue in Picea omorika Culture." Biologia plantarum 46, no. 3 (November 1, 2003): 467–70. http://dx.doi.org/10.1023/b:biop.0000023898.83886.7d.

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GEBHARDT, C., R. SCHMIDT, and K. SCHNEIDER. "Plant Genome Analysis: The State of the Art." International Review of Cytology 247 (2005): 223–84. http://dx.doi.org/10.1016/s0074-7696(05)47005-9.

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25

La Rosa, Rafael, Maria Sánchez, and Eleucy Pérez. "Internal morphology and histology of blueberry Vaccinium corymbosum L. (Ericaceae) in Lima, Peru." Agronomía Colombiana 35, no. 2 (May 1, 2017): 176–81. http://dx.doi.org/10.15446/agron.colomb.v35n2.63146.

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Although there is a lot of information about cultivation, use and medicinal properties of blueberry Vaccinium corymbosum, there is still little information about its internal morphology and histology. Therefore, we proposed to know more of those aspects and to understand the low seed germination. The material used was composed of seeds and mature plants obtained from a farm located in Trujillo, Peru. All histological work was made in the Laboratory of Plant Anatomy and Pharmacognosy belongs to Faculty of Biological Science in Universidad Nacional Mayor de San Marcos in Lima, Peru. Each organ was analyzed in cross and longitudinal sections, as well as in external or superficial view. Lugol and Sudan IV were used for seed sections, Safranin for stem and root sections, and Lugol for leaf sections. We found some variation in seed size and color, being assigned to the category of oil seeds. Germination was limited by the embryos viability, as well as thickness of the seed coat. Stems and roots have punctuated xylem vessels, which facilitate the lateral water transport. The radical system is highly branched, apparently due to mycorrhizal symbiosis. Leaves are bifacial, with all the stomata on the abaxial side, and features that are characteristic of C3 plants.

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Vitha, Stanislav, František Baluška, Miriam Mews, and Dieter Volkmann. "Immunofluorescence Detection of F-actin on Low Melting Point Wax Sections from Plant Tissues." Journal of Histochemistry & Cytochemistry 45, no. 1 (January 1997): 89–95. http://dx.doi.org/10.1177/002215549704500112.

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We developed a simple and reliable technique for immunofluorescence detection of F-actin on microtome sections of plant tissues. For the first time, large numbers of plant cells from various tissues that pass through their developmental stages could be consistently visualized on one section from plant organs. n-Maleimidobenzoic acid N-hydroxy-succinimide ester-pretreated and formalin-fixed segments of plant roots and shoots were embedded in low melting point ester wax at 37C and sectioned on a microtome. After dewaxing and rehydration, microfilaments were visualized by indirect immunofluorescence technique with a monoclonal anti-actin antibody. The technique has been successfully used for visualization of tissue- and development-specific F-actin arrays in cells of Zea mays and Lepidium sativum root tips and of maize stem nodes.

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Sharma, K. "Histology of Shoot Bud Ontogeny from Seedling Root Segments of Brassica napus L." Annals of Botany 71, no. 5 (May 1993): 461–66. http://dx.doi.org/10.1006/anbo.1993.1060.

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Reig-Armiñana, J., V. Calatayud, J. Cerveró, F. J. Garcı́a-Breijo, A. Ibars, and M. J. Sanz. "Effects of ozone on the foliar histology of the mastic plant (Pistacia lentiscus L.)." Environmental Pollution 132, no. 2 (November 2004): 321–31. http://dx.doi.org/10.1016/j.envpol.2004.04.006.

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Romanov, Mikhail S., Peter K. Endress, Alexey V. F. Ch Bobrov, Anton A. Yurmanov, and Ekaterina S. Romanova. "Fruit Structure of Calycanthaceae (Laurales): Histology and Development." International Journal of Plant Sciences 179, no. 8 (October 2018): 616–34. http://dx.doi.org/10.1086/699281.

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Grimault, V., B. Gélie, M. Lemattre, P. Prior, and J. Schmit. "Comparative histology of resistant and susceptible tomato cultivars infected by Pseudomonas solanacearum." Physiological and Molecular Plant Pathology 44, no. 2 (February 1994): 105–23. http://dx.doi.org/10.1016/s0885-5765(05)80105-5.

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Simmonds, D. H., R. W. Seagull, and G. Setterfield. "Evaluation of techniques for immunofluorescent staining of microtubules in cultured plant cells." Journal of Histochemistry & Cytochemistry 33, no. 4 (April 1985): 345–52. http://dx.doi.org/10.1177/33.4.2579999.

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Various modifications to the immunofluorescent labeling procedures for microtubules in plant cells have been compared using cell cultures of Vicia hajastana Grossh. Using serial section electron microscopic reconstructions as a reference, we have chosen as our standard procedure a method that maximizes both the preservation of the cytoskeleton and the proportion of cells staining, while minimizing the degree of nonspecific staining. The critical steps of the procedure include stabilization of the cytoskeleton, cell wall permeabilization, and cell extraction. To maintain structural integrity during the procedure, it is necessary to stabilize the cytoskeleton with paraformaldehyde. To facilitate antibody penetration into the cell, it was necessary that the walls be made permeable via partial enzymatic digestion. Detergent extraction of cells increased the proportion of cells staining and decreased the level of nonspecific binding of the antibodies. The procedures detailed in this article provide a good starting point for the application of immunofluorescent labeling techniques to other plant systems.

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Schaumann, L., P. Galle, M. Thellier, and J. C. Wissocq. "Imaging the distribution of the stable isotopes of nitrogen 14N and 15N in biological samples by "secondary-ion emission microscopy"." Journal of Histochemistry & Cytochemistry 36, no. 1 (January 1988): 37–39. http://dx.doi.org/10.1177/36.1.3335768.

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Thanks to the "secondary-ion emission microscope" (CAMECA IMS 300), we have been able to image the distribution of the stable isotopes of nitrogen 14N and 15N in sections of plant roots (spatial resolution better than 1 micron), as well as to estimate the relative concentrations of these isotopes. The plants used (Lupinus spec.) originated from seeds with natural (i.e., 14N) nitrogen and had been fed for a few days with [15N]-nitrate before sampling. We have found in root sections of 6-day-old plants (prepared at 5 mm from the root tip) a clear-cut regionalization of the distribution of 15N between the vascular cylinder and the cortex. The latter contained approximately 5% 15N (of total nitrogen), whereas the relative concentration of the heavy isotope in the vascular cylinder was significantly lower. The observed concentration difference is probably due to the Casparian strip, which is a barrier for the apoplastic diffusion of solutes from the cortex to the vascular cylinder.

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Creemers-Molenaar, J. "Histology of the Morphogenic Response in Thin Cell Layer Explants from Vegetative Tobacco Plants." Annals of Botany 73, no. 5 (May 1994): 547–55. http://dx.doi.org/10.1006/anbo.1994.1068.

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TIMMERS, ANTONIUS C. J. "Light microscopy of whole plant organs." Journal of Microscopy 263, no. 2 (March 29, 2016): 165–70. http://dx.doi.org/10.1111/jmi.12394.

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Kaeser, Wulf, Hans-Werner Koyro, and Hans Moor. "Cryofixation of plant tissues without pretreatment." Journal of Microscopy 154, no. 3 (June 1989): 279–88. http://dx.doi.org/10.1111/j.1365-2818.1989.tb00591.x.

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TÃ¡rnok, Attila. "Visiting the plant kingdom." Cytometry Part A 75A, no. 12 (December 2009): 973–74. http://dx.doi.org/10.1002/cyto.a.20829.

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Cruthers, Natasha, Debra Carr, Brian Niven, Elizabeth Girvan, and Raechel Laing. "Methods for characterizing plant fibers." Microscopy Research and Technique 67, no. 5 (2005): 260–64. http://dx.doi.org/10.1002/jemt.20206.

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Nedukha, O. M. "Callose: Localization, functions, and synthesis in plant cells." Cytology and Genetics 49, no. 1 (January 2015): 49–57. http://dx.doi.org/10.3103/s0095452715010090.

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Samofalova, D. A., P. A. Karpov, and Ya B. Blume. "Bioinformatic comparison of human and higher plant phosphatomes." Cytology and Genetics 49, no. 4 (July 2015): 207–19. http://dx.doi.org/10.3103/s0095452715040088.

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Hussein, Mahmoud Elnahas. "Manfort, a blend from plant extracts used for infertility treatment and improvement of testicular histology." International Journal of Reproduction, Contraception, Obstetrics and Gynecology 9, no. 1 (December 26, 2019): 352. http://dx.doi.org/10.18203/2320-1770.ijrcog20196047.

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Background: Flavonoids and polyphenols are groups of natural substances have variable phenolic configurations, many benefits as anti-inflammatory and anti-oxidants, and have many protective roles against male reproductive system disorders. Objective of this study was to study the safety as well as the efficacy of a blend from some plants extracts with precise ratios and rich with flavonoids and polyphenols named in this study as “Manfort” on the safety, fertility, and testicular histology in the male mice.Methods: Firstly, some mice were used to evaluate the safety of Manfort and the levels of the testosterone in the serum of the treated animals with Manfort using biochemical analysis. Also, the efficacy of the Manfort on the histological architectures of the treated testis was evaluated using histological techniques.Results: The mice treated with Manfort did not show any signs of mortality, toxicities and blood contents changes. Furthermore, testosterone levels in the serum elevated after administration with Manfort twice a day for 21 days compared with the non-treated mice. Additionally, the histological structures of the testis improved in the mice treated with Manfort compared with that in the non-treated animals.Conclusions: In general flavonoids and polyphenols, which were found in the Manfort diet in a large amount, have important role in as antioxidant, anti-inflammatory and improve the fertility in the male mice. In the future these data might be very important to manufacture a drug composed from safe natural products for infertility and intractable diseases treatment.

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Yeung, Edward C. "The use of histology in the study of plant tissue culture systems—Some practical comments." In Vitro Cellular & Developmental Biology - Plant 35, no. 2 (March 1999): 137–43. http://dx.doi.org/10.1007/s11627-999-0023-z.

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Gasmalbari, Elmuaiz, Hatil H. EL-Kamali, and Osama S. Abbadi. "Biochemical and Haematological Effects and Histopathological Changes caused by Moringa oleifera on Albino Rats." Chinese Journal of Medical Research 3, no. 3 (September 25, 2020): 84–88. http://dx.doi.org/10.37515/cjmr.091x.3306.

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Background: Of the plant family Moringaceae, Moringa oleifera is the most popular. It draws researchers’ attention by its nutritional and phytochemical properties. The potential effects of this plant are still to be explored. This study aimed to evaluate the effect of Moringa olifiera in the blood glucose, serum lipids, hemoglobin, hematologic values of blood cells, and the possible organ toxicity of this plant by measuring liver and kidney functions and histology. Materials and method: Moringa olifiera leaves were pounded to powder using a pestle. 100mg for each kilogram of bodyweight (mg/kg/bw) were weighted for each rat. Sera for blood glucose, lipid profile, blood parameters, and histology slides for liver and kidneys were prepared and studied after the above dose administration-orally, for three weeks. Results: Administration of aqueous Moringa leaves100mg/kg/bw to rats for 21days caused a significant decrease in the triglycerides level, plasma cholesterol, blood glucose, platelets count, plasma proteins and albumin. There was a significant increase in the body weight. White blood cells (WBCs) and packed cell volume (PCV) increased significantly, but the changes in Red blood cells (RBCs), haemoglobin level, blood urea and creatinine were not statistically significant. Features of tissue injury were seen in the liver and renal histology slides. Conclusion: The effects of Moringa olifiera in albino rats in this study agrees with most of the previous researches, but contradicts the literature with regards to the cholesterol and renal function results.

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Weiland, Jerry E., and Glen R. Stanosz. "The Histology of Hybrid Poplar Clones Inoculated with Septoria musiva." Plant Disease 91, no. 12 (December 2007): 1524–30. http://dx.doi.org/10.1094/pdis-91-12-1524.

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Septoria musiva causes stem cankers that severely limit production of susceptible hybrid poplars in eastern North America. A field experiment was conducted with resistant clone DN34 and susceptible clone NC11505 in order to (i) identify tissues colonized by the pathogen, (ii) describe tissue responses to S. musiva, and (iii) determine whether tissue responses to S. musiva differed between hybrid poplar clones. Branches of each clone were inoculated by removing the fourth or fifth fully expanded leaf and placing an agar plug colonized by an aggressive isolate of S. musiva over the wound. Seven weeks after inoculation, branches were harvested and prepared for histology. Data from nonwounded control, wounded control, and wounded and inoculated stems were collected and analyzed for effects of clone and treatment. In general, fungal colonization was more extensive in NC11505 and exophylactic and necrophylactic periderms (NPs) of clone DN34 were significantly thicker than those of NC11505, regardless of treatment. The number of NPs produced and the distance from the epidermis to the outermost layer of phellem were significantly affected by the pathogen. Inoculated stems of clone DN34 developed a single NP that formed closer to the wound surface than in wounded controls. In contrast, inoculated stems of NC11505 developed successive NPs and the first NP formed further from the wound surface than in wounded controls. These two host responses to inoculation, as well as measures of exophylactic and necrophylactic periderm thickness, may be useful as markers for the selection of poplar resistant to damage by S. musiva.

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Nin, Gerardo H. Vázquez, Olga M. Echeverría, Guadalupe Zavala, Luis F. J. Jiménez-García, Marco A. Gozalez, and Rosario Parra. "Relations between Nucleolar Morphometric Parameters and Pre-rRNA Synthesis in Animal and Plant Cells." Cells Tissues Organs 126, no. 3 (1986): 141–46. http://dx.doi.org/10.1159/000146203.

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Harris, N. "Molecular histochemistry and plant biology: a review." Journal of Microscopy 166, no. 1 (April 1992): 3–14. http://dx.doi.org/10.1111/j.1365-2818.1992.tb01503.x.

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BOUCHEKHIMA, A. N., L. FRIGERIO, and &. M. KIRKILIONIS. "Geometric quantification of the plant endoplasmic reticulum." Journal of Microscopy 234, no. 2 (May 2009): 158–72. http://dx.doi.org/10.1111/j.1365-2818.2009.03158.x.

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Stano, Ján, Peter Kovács, Karol Mičieta, Klaus Neubert, Herbert Tintemann, and Marcela Koreňová. "Localization and measurement of extracellular plant galactosidases." Acta Histochemica 104, no. 4 (January 2002): 441–44. http://dx.doi.org/10.1078/0065-1281-00665.

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Lu, Chin-yi, and Indra K. Vasil. "HISTOLOGY OF SOMATIC EMBRYOGENESIS IN PANICUM MAXIMUM (GUINEA GRASS)." American Journal of Botany 72, no. 12 (December 1985): 1908–13. http://dx.doi.org/10.1002/j.1537-2197.1985.tb08464.x.

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Korban, S. S., and S. E. Riemer. "Genetics and histology of powdery mildew resistance in apple." Euphytica 48, no. 3 (July 1990): 261–67. http://dx.doi.org/10.1007/bf00023659.

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Fedak, G., and N. S. Kim. "Tools and methodologies for cytogenetic studies of plant chromosomes." Cytology and Genetics 42, no. 3 (June 2008): 189–203. http://dx.doi.org/10.3103/s0095452708030067.

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Journal articles on the topic 'Plant histology'

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