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1

Keet, Jan-Hendrik, and David M. Richardson. "A rapid survey of naturalized and invasive eucalypt species in southwestern Limpopo, South Africa." South African Journal of Botany 144 (January 2022): 339–46. http://dx.doi.org/10.1016/j.sajb.2021.09.008.

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2

Maema, Lesibana Peter, Martin Potgieter, and Salome Mamokone Mahlo. "INVASIVE ALIEN PLANT SPECIES USED FOR THE TREATMENT OF VARIOUS DISEASES IN LIMPOPO PROVINCE, SOUTH AFRICA." Africa Journal of Traditional Complementary and Alternative Medicine 13, no. 4 (July 3, 2016): 223–31. http://dx.doi.org/10.21010/ajtcam.v13i4.29.

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3

Kurugundla, C. N., B. Mathangwane, S. Sakuringwa, and G. Katorah. "Alien Invasive Aquatic Plant Species in Botswana: Historical Perspective and Management." Open Plant Science Journal 9, no. 1 (June 14, 2016): 1–40. http://dx.doi.org/10.2174/1874294701609010001.

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Aquatic ecosystems in Botswana have been under threat by the aquatic alien invasive plant species viz., salvinia Salvinia molesta Mitchell, water lettuce Pistia stratiotes L., and water hyacinth Eichhornia crassipes (Mart.) Solms-Laub. While salvinia has been termed the major threat to the Botswana wetlands, water lettuce and water hyacinth are considered to be of minor importance. This review presents the species biology, distribution, historical spread, negative impacts, control achieved right from their discovery in the country by referring to their control and management in the world. Having infested the Kwando-Linyanti-Chobe Rivers in the 1970s, salvinia was initially tried by the use of herbicides, paraquat and glyphosate, between 1972 and 1976. With the discovery of the host specific biological control weevil Cyrtobagous salviniae Calder and Sands in 1981, the weevil was introduced by Namibians on Kwando and Chobe Rivers in 1983 and by Botswana in 1986 in the Okavango Delta. While the control was slowly establishing in Kwando-Linyanti-Chobe Rivers, it became apparent that lakes and perennial swamps within and outside Moremi Game Reserve of the Okavango Delta were infested with salvinia from 1992 onwards. With continuous and sustained liberation of the weevil in the Kwando-Linyanti-Chobe Rivers and in the Okavango Delta between 1999 and 2000, salvinia control was achieved by 2003, and since then the weevil constantly keeps the weed at low levels. The success is mainly due to sustainable monitoring through the application of physical and biological control methods. However, salvinia is still threatening the Okavango Delta due to factors such as tourism activities, boat navigation fishing and transporttion by wild animals. The first occurrence of water lettuce was recorded on Kwando and Chobe Rivers in 1986. Its biocontrol weevil Neohydronomous affinis Hustache was released in the year 1987. The weevil became extinct in Selinda Canal and Zibadianja Lake on Kwando River due to dry and wet events for over 10 years and the weed had been under control biologically on Chobe River. Having surface covered the Selinda and a part of the Zibadianja in high flood and rainfall in 1999/2000 season, research was undertaken to contain water lettuce, which led to its eradication by 2005. Regular physical removal of the water lettuce prior to fruit maturity is an effective method of control or eradicating the weed in seasonal water bodies. The Limpopo Basin (shared by Botswana, South Africa, Zimbabwe and Mozambique) has become vulnerable to water hyacinth infestation. Water hyacinth infested the trans-boundary Limpopo River in 2010 sourced from Hartbeesport Dam on Crocodile River in South Africa. Botswana and South Africa have been consulting each other to implement integrated control of the weed jointly in the Limpopo River. Water hyacinth could be a continuous threat to the dams and the rivers in the Limpopo basin if its control is not taken seriously. These three species are found growing in Botswana in a range of pH between 4.5 and 10.3 and in the range of conductivities between 20 and 580 µS cm-1. Range of soluble nitrates, phosphates and potassium in the habitats of salvinia infestations were 0.02 to 1.5, 0.01 to 1.78 and 0.3 to 6.92 mg L-1 respectively. Water lettuce infestation in the seasonal Selinda Canal had a maximum of 4.7 mg L-1 nitrates, 2.8 mg L-1 phosphates and 7.9 mg L-1 potassium. Nevertheless, these three nutrients were in the range of 0.41 to 9.56 mg L-1, 0.2 to 2.9 mg L-1, and 7.7 to 11.53 mg L-1 respectively in the Limpopo River where water hyacinth infestations were observed. These nutrients were considerably high during decomposition phase of biological control of weeds. The Government of Botswana “regulates the movement and importation of boats and aquatic apparatus, to prevent the importation and spread of aquatic weeds both within and from the neighboring countries” by “Aquatic Weed (Control) Act” implemented in 1986. These measures, combined with communities, conservation groups, NGOs and public awareness campaigns, have highlighted the gravity of aquatic weeds spreading into wetlands, dams and other water bodies. In conclusion, the Government of Botswana is committed and supportive through the Department of Water Affairs in protecting the wetlands of the country efficiently and prudently.
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4

Ndou, S., and M. H. Ligavha-Mbelengwa. "Survival strategies of selected alien invasive plants in parts of Thulamela Local Municipality, Limpopo Province, South Africa." South African Journal of Botany 109 (March 2017): 359. http://dx.doi.org/10.1016/j.sajb.2017.01.143.

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5

Mbedzi, M., M. P. Tshisikhawe, N. I. Sinthumule, and S. Rahlao. "The rate of regeneration of native plant species after the eradication of invasive alien plant species in Limpopo Province, South Africa." South African Journal of Botany 115 (March 2018): 298. http://dx.doi.org/10.1016/j.sajb.2018.02.081.

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6

Nenzhelele, E., and M. P. Tshisikhawe. "The diversity and the spread of alien invasive plant species in the Nylsvley Nature Reserve, Limpopo Province, South Africa." South African Journal of Botany 98 (May 2015): 213–14. http://dx.doi.org/10.1016/j.sajb.2015.03.167.

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7

Ramarumo, Luambo Jeffrey, Alfred Maroyi, and Milingoni Peter Tshisikhawe. "Plant species used for birdlime-making in South Africa." Bangladesh Journal of Botany 49, no. 1 (March 31, 2020): 117–24. http://dx.doi.org/10.3329/bjb.v49i1.49104.

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Plants used for making birdlime and indigenous knowledge associated with the practice in Soutpansberg region, Vhembe Biosphere Reserve, Limpopo province, South Africa have been documented. Twelve birdlime-making plant species belonging to Apocynaceae, Celastraceae, Euphorbiaceae, Loranthaceae, Moraceae and Sapotaceae families were recorded. The common species included Maytenus peduncularis (Sond.) Loyes cited by 23.6% informants, Euphorbia pulvinata Marloth (17.2%) and Landolphia kirkii Dyer (12%). Plant parts used were latex (50%), fruit (34%), root (8) and the mixture of latex and fruit (8%). Documentation of plant species used for birdlime-making is of great interest, not only for preservation of the Vhavenḓa's traditional culture, but also for promoting economic subsistence, nutritional value and livelihood amongst poor and marginalized people.
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8

Ruwanza, Sheunesu, and Farai Dondofema. "Effects of exotic guava ( Psidium guajava L.) invasion on soil properties in Limpopo, South Africa." African Journal of Ecology 58, no. 2 (July 21, 2019): 272–80. http://dx.doi.org/10.1111/aje.12675.

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9

Hahn, N., and G. J. Bredenkamp. "LAMIACEAE." Bothalia 37, no. 1 (August 18, 2007): 37–40. http://dx.doi.org/10.4102/abc.v37i1.298.

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10

Klopper, R. R., and A. E. Van Wyk. "Pteridophyta–Sinopteridaceae." Bothalia 41, no. 1 (December 13, 2011): 204–7. http://dx.doi.org/10.4102/abc.v41i1.51.

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11

Hurter, P. J. H., and A. E. Hurter. "FABACEAE." Bothalia 34, no. 2 (September 3, 2004): 109–12. http://dx.doi.org/10.4102/abc.v34i2.422.

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12

Jordaan, M., and H. M. Steyn. "Rubiaceae." Bothalia 42, no. 2 (December 9, 2012): 204–9. http://dx.doi.org/10.4102/abc.v42i2.21.

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13

Chauke, MA, LJ Shai, MA Mogale, MP Tshisikhawe, and MP Mokgotho. "Medicinal plant use of villagers in the Mopani district, Limpopo province, South Africa." African Journal of Traditional, Complementary and Alternative Medicines 12, no. 3 (July 30, 2015): 9. http://dx.doi.org/10.4314/ajtcam.v12i3.2.

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14

Klopper, R. R., and G. F. Smith. "Asphodelaceae: Alooideae - Aloe Hahnii, a new species in the section Pictae, in the Soutpansberg centre of endemism, Limpopo Province, South Africa." Bothalia 39, no. 1 (August 11, 2009): 98–100. http://dx.doi.org/10.4102/abc.v39i1.233.

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15

BIDZILYA, OLEKSIY. "New host-plants records of Afrotropical Gelechiidae (Lepidoptera), with description of three new species." Zootaxa 4952, no. 3 (April 12, 2021): 495–522. http://dx.doi.org/10.11646/zootaxa.4952.3.4.

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First and additional host-plant records for 38 species of Gelechiidae from South Africa and Kenya are presented. An annotated list with brief descriptions of larval biology and known geographical distributions is presented. Three species are described as new: Mesophleps kruegeri sp. nov. (Namibia: Kavango Region and South Africa: Limpopo Province), Istrianis inquilinus sp. nov. (South Africa: North West Province), and Teleiopsis sharporum sp. nov. (South Africa: Limpopo Province). The new combination Istrianis epacria (Bradley, 1965) comb. nov. is proposed. The male genitalia of Hypatima melanecta (Meyrick, 1914) is described for the first time. It is noticed that male hitherto associated with T. commaculata (Meyrick, 1918) in fact refers to T. pundamilia Bidzilya & Mey, 2018. Hypatima stasimodes (Meyrick, 1931), Dichomeris coenulenta (Meyrick, 1927), Dichomeris eustacta Meyrick, 1921, Neotelphusa similella Janse, 1958 and Argophara epaxia Janse, 1963 are recorded for the first time from South Africa, and Istrianis epacria is new for Kenya. The adults, male and female genitalia are illustrated for some species mentioned in paper. The first record of the genus Teleiopsis Sattler, 1960 from South Africa is briefly discussed.
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16

Rasethe, Marula Triumph, Sebua Silas Semenya, and Alfred Maroyi. "Medicinal Plants Traded in Informal Herbal Medicine Markets of the Limpopo Province, South Africa." Evidence-Based Complementary and Alternative Medicine 2019 (April 16, 2019): 1–11. http://dx.doi.org/10.1155/2019/2609532.

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Trading of herbal medicines generates economic opportunities for vulnerable groups living in periurban, rural, and marginalized areas. This study was aimed at identifying medicinal plant species traded in the Limpopo province in South Africa, including traded plant parts, conservation statutes of the species, and harvesting methods used to collect the species. Semistructured questionnaire supplemented by field observation was used to collect data from owners of 35 informal herbal medicine markets in the Limpopo province. A total of 150 medicinal plant products representing at least 79 plant species belonging to 45 botanical families, mainly the Fabaceae (11.4%), Asteraceae (7.6%), and Hyacinthaceae (6.3%), were traded in the study area. Roots (50.0%), bulbs (19.0%), and bark (16.0%) were the most frequently sold plant parts. Some of the traded species which includeAlepidea amatymbica, Bowiea volubilis, Brackenridgea zanguebarica, Clivia caulescens,Dioscorea sylvatica,Elaeodendron transvaalense, Encephalartos woodii,Eucomis pallidiflorasubsp.pole-evansii,Merwilla plumbea,Mondia whitei,Prunus africana, Siphonochilus aethiopicus, Synaptolepis oliveriana,andWarburgia salutarisare of conservation concern and listed on the South African Red Data List.Findings of this study call for effective law enforcement to curb illegal removal of wild plants especially those species that are at the verge of extinction.
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17

Makonya, G. M., J. B. O. Ogola, M. A. Muasya, O. Crespo, and S. B. M. Chimphango. "Physiological response for thermotolerant Chickpea (Cicer arietinum L.; Fabaceae) genotypes in Limpopo, South Africa." South African Journal of Botany 115 (March 2018): 295–96. http://dx.doi.org/10.1016/j.sajb.2018.02.073.

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18

Mabapa, Moshibudi Paulina, Kingsley Kwabena Ayisi, Irvine Kwaramba Mariga, Ramasela Charlotte Mohlabi, and Richard Sello Chuene. "Production and Utilization of Moringa by Farmers in Limpopo Province, South Africa." International Journal of Agricultural Research 12, no. 4 (September 15, 2017): 160–71. http://dx.doi.org/10.3923/ijar.2017.160.171.

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19

Lemmer, P. "Jamesbrittenia bergae (Scrophulariaceae), a distinctive new species from Limpopo, South Africa." Bothalia 33, no. 2 (September 9, 2003): 141–43. http://dx.doi.org/10.4102/abc.v33i2.441.

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Jamesbrittenia bergae P.Lemmer is a new species from the Farm Brakvallei near Thabazimbi in Limpopo [Northern Province]. Large, medium-textured. bright scarlet flowers w ith yellow throats are borne singly in upper leaf axils; the flowering branches, although terminal, do not form elongated racemes: leaves are greyish green, coarsely serrated. It grows in full sun on ferricrete outcrops.
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20

Richardson, D. M. "Invasion science for society: Challenges and opportunities in South Africa." South African Journal of Botany 103 (March 2016): 304. http://dx.doi.org/10.1016/j.sajb.2016.02.005.

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21

Van der Linde, J. A., D. Six, M. J. Wingfield, and J. Roux. "Consideration of factors associated with Euphorbia ingens decline in the Limpopo Province of South Africa." South African Journal of Botany 76, no. 2 (April 2010): 404–5. http://dx.doi.org/10.1016/j.sajb.2010.02.049.

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22

Morokong, T., J. N. Blignaut, N. P. Nkambule, T. Vundla, and S. Mudavanhu. "Assessing the viable agricultural land use options after clearing invasive alien plants in the Mokolo Catchment, Limpopo Province, South Africa." Agrekon 57, no. 3-4 (October 2, 2018): 266–83. http://dx.doi.org/10.1080/03031853.2018.1530124.

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23

MQ, Maanda, and RB Bhat. "Wild vegetable use by Vhavenda in the Venda region of Limpopo Province, South Africa." Phyton 79, no. 1 (2010): 189–94. http://dx.doi.org/10.32604/phyton.2010.79.189.

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24

BOTHA, J., E. T. F. WITKOWSKI, and C. M. SHACKLETON. "Market profiles and trade in medicinal plants in the Lowveld, South Africa." Environmental Conservation 31, no. 1 (March 2004): 38–46. http://dx.doi.org/10.1017/s0376892904001067.

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Rising demand for medicinal plants has led to increased pressure on wild plant populations. This, combined with shrinking habitats, means that many species in South Africa are now facing local extinction. In 1997, a study was initiated to determine the extent of trade in medicinal plants in the South African Lowveld (the low lying plains to the east of the Drakensberg escarpment), and to investigate socio-economic factors influencing trade and resource management. Trade was not as extensive in the Lowveld as in major urban markets such as Durban or the Witwatersrand (Johannesburg and surrounding towns), either in terms of the quantity, number or range of species sold, or the numbers of people relying on the trade for an income. In markets assessed in Mpumalanga Province, 176 species were identified (71% of the vernacular names encountered in the market place), representing 69 plant families. In Limpopo, 70 different species were identified (84% of the vernacular names encountered in the market place), representing 40 families. Imports were significant in Mpumalanga (33% of the plants on offer), mainly from Mozambique. A detrended correspondence analysis showed substantial differences between species traded in Mpumalanga and those sold in Limpopo. There was little variation in the species stocked by vendors in Mpumalanga, regardless of the season, the attributes of the seller, or whether business was carried out in urban or rural areas. In contrast, there was considerable variation in the stock inventories of the Limpopo traders. Despite the lower levels of local trade, increased harvesting pressure is being experienced regionally, to meet demand in metropolitan centres such as the Witwatersrand. This study showed considerable local variation and complexities in the harvesting and marketing of medicinal plants, with both a national and an international dimension. This dual spatial scale presents both opportunities and challenges in the management of these plants, which need to be addressed simultaneously, particularly with respect to research requirements and development of predictive models and capacity. Cooperation in conservation strategies and policies is required at regional, national and international levels, while ensuring that management initiatives take into account local market conditions and the socio-economic realities facing both consumers and those who depend on the trade for their livelihoods.
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25

Vardien, W., D. M. Richardson, L. C. Foxcroft, G. D. Thompson, J. R. U. Wilson, and J. J. Le Roux. "Invasion dynamics of Lantana camara L. (sensu lato) in South Africa." South African Journal of Botany 81 (July 2012): 81–94. http://dx.doi.org/10.1016/j.sajb.2012.06.002.

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26

Mashile, S. P., M. P. Tshisikhawe, and M. H. Ligavha-Mbelengwa. "Tree layer species composition of the Nylsvley Nature Reserve woodland community in Limpopo Province, South Africa." South African Journal of Botany 76, no. 2 (April 2010): 413. http://dx.doi.org/10.1016/j.sajb.2010.02.074.

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27

Tshikhudo, P. P., K. Ntushelo, S. A. Kanu, and F. N. Mudau. "Growth response of bush tea (Athrixia phylocoides DC.) to climatic conditions in Limpopo Province, South Africa." South African Journal of Botany 121 (March 2019): 500–504. http://dx.doi.org/10.1016/j.sajb.2018.12.012.

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28

SEMENYA, SEBUA SILAS, and ALFRED MAROYI. "Source, harvesting, conservation status, threats and management of indigenous plant used for respiratory infections and related symptoms in the Limpopo Province, South Africa." Biodiversitas Journal of Biological Diversity 20, no. 3 (March 3, 2019): 789–810. http://dx.doi.org/10.13057/biodiv/d200325.

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Abstract. Semenya SS, Maroyi A. 2019. Source, harvesting, conservation status, threats and management of indigenous plant used for respiratory infections and related symptoms in the Limpopo Province, South Africa. Biodiversitas 20: 789-810. This survey explored Bapedi traditional healer’s (THs) practices pertinent to native plants used to treat respiratory infections (RIs) and related symptoms (RSs). Semi-structured questionnaires and participatory observations were used to gather information from 240 THs in the Limpopo Province, South Africa. 186 plants from 75 families were harvested by these THs, mainly from the communal lands (81.2%), throughout the year. Plant parts used for RIs and RSs remedies was destructively harvested in wilderness compared to homegardens. Most (n=174) species from which these parts are obtained appears on the South African National Red Data List of plants, with 88.5% having a list concern status. This included Adansonia digitata, Boscia albitrunca, Catha edulis, Securidaca longepedunculata and Sclerocarya birrea which are also protected under the National Forest Act of 1998 (Act no. 84 of 1998). A further, 8.6% (n=15) of Red Data Listed plants are of conservation concern, with various status namely near threatened (38.3%), declining (20%), data deficient (13.3%), critically endangered and vulnerable (3.3%, for each), as well as endangered (6.6%). There were both consensus and disjunction amongst THs and Red Data List regarding the status of plants in the wild. This study provides valuable data for the conservation of medicinal plants in Limpopo Province.
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29

Maluleke, Witness, Ntwanano Patrick Tshabalala, and Jaco Barkhuizen. "The Effects of Climate Change on Rural Livestock Farming: Evidence from Limpopo Province, South Africa." Asian Journal of Agriculture and Rural Development 10, no. 2 (September 1, 2020): 645–58. http://dx.doi.org/10.18488/journal.ajard.2020.102.645.658.

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The objective of this study is to identify the perceptions of the conspicuous rural livestock farmers as well as the officials from the Department of Agriculture, Forestry and Fisheries (DAFF) on the effects of climate change on rural livestock farming practices. From a qualitative standpoint, this study purposively selected participants from Limpopo Province, focusing on Giyani, Lenting, Ga-Mphahlele and Malamulele areas comprising of rural livestock farmers [12:3 = 36] and DAFF officials [6:2]. Overall, 42 participants formed part of this study through Face-to-Face and Focus Group Discussions. It is found that the loss and damage related to the contrary effects of climate change are insufficiently applied in the chosen rural areas of Limpopo Province. The local and regional collaborations by the responsible spheres of government are not strengthened and promoted, leading to inefficient strategies and approaches to addressing related conditions. Therefore, the significance of risk transmission and dissemination via regional cooperation regarding climate change adaptation are pivotal in rural settings.
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Rasethe, Marula T., Sebua S. Semenya, Martin J. Potgieter, and Alfred Maroyi. "The utilization and management of plant resources in rural areas of the Limpopo Province, South Africa." Journal of Ethnobiology and Ethnomedicine 9, no. 1 (2013): 27. http://dx.doi.org/10.1186/1746-4269-9-27.

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Mosina, Gabolwelwe KE, Alfred Maroyi, and Martin J. Potgieter. "Comparative analysis of plant use in peri-urban domestic gardens of the Limpopo Province, South Africa." Journal of Ethnobiology and Ethnomedicine 10, no. 1 (2014): 35. http://dx.doi.org/10.1186/1746-4269-10-35.

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Ramovha, L. I. "The ethno-veterinary utilization of Lonchocarpus capassa Rolfe by the Vhavenda in Vhembe District, Limpopo, South Africa." South African Journal of Botany 75, no. 2 (April 2009): 417. http://dx.doi.org/10.1016/j.sajb.2009.02.096.

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Seloana, M. Q., M. J. Potgieter, J. W. Kruger, and J. J. Jordaan. "The ecological impact of elephant herbivory on vegetation of Atherstone Collaborative Nature Reserve, Limpopo Province, South Africa." South African Journal of Botany 103 (March 2016): 348. http://dx.doi.org/10.1016/j.sajb.2016.02.166.

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34

Semenya, Sebua Silas, and Alfred Maroyi. "Medicinal uses of alien plants cultivated and managed in homegardens of Limpopo province, South Africa." Medicinal Plants - International Journal of Phytomedicines and Related Industries 12, no. 3 (2020): 429. http://dx.doi.org/10.5958/0975-6892.2020.00054.4.

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Semenya, Sebua Silas, and Alfred Maroyi. "Medicinal plants used for eye disorders and chest pains in the Limpopo province, South Africa." Medicinal Plants - International Journal of Phytomedicines and Related Industries 12, no. 4 (2020): 623–32. http://dx.doi.org/10.5958/0975-6892.2020.00075.1.

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36

SMITH, GIDEON F. "Aloe ×retiefii (Asphodelaceae subfam. Alooideae), a new nothospecies from northeastern South Africa, with A. globuligemma and A. marlothii as parents." Phytotaxa 468, no. 1 (October 23, 2020): 150–54. http://dx.doi.org/10.11646/phytotaxa.468.1.12.

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Aloe marlothii Berger (1905: 87) (Asphodelaceae subfam. Alooideae) (Fig. 1), a single-stemmed, tree-like aloe, occurs widespread in northeastern South Africa and adjacent parts of Botswana, for example near Lobatse in the southeast of the country from where it was originally described (Fig. 2), Zimbabwe, and Mozambique (see maps in Klopper & Smith 2010: 92, Fig. 32 and Van Wyk & Smith 2014: 68). Wherever A. marlothii co-occurs with other species of Aloe Linnaeus (1753: 319), hybrids are produced (Reynolds 1950, Smith & Figueiredo 2015). Aloe globuligemma Pole-Evans (1915: 30, Plates X [Figs 1 and 2] and XI [Figs 1, 2, and 3]) (Fig. 3), a stemless, medium-sized aloe, is restricted to the more subtropical parts of northeastern South Africa, southern Zimbabwe, and Mozambique (see map in Van Wyk & Smith 2014: 158). Where these two species, A. globuligemma and A. marlothii, co-occur, for example at Lebowakgomo, about 50 km southeast of Polokwane, the capital of South Africa’s Limpopo province, a bewildering range of striking hybrids with considerable horticultural potential are found (Reynolds 1950: 446, 484, Retief 2018). This hybrid also occurs a bit further south of the Lebowakgomo location, as well as elsewhere in the Limpopo province, in Sekhukhuneland in the vicinity of Steelpoort and Ohrigstad.
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37

Mbambala, S. G., M. P. Tshisikhawe, and N. A. Masevhe. "INVASIVE ALIEN PLANTS USED IN THE TREATMENT OF HIV/AIDS-RELATED SYMPTOMS BY TRADITIONAL HEALERS OF VHEMBE MUNICIPALITY, LIMPOPO PROVINCE, SOUTH AFRICA." African Journal of Traditional, Complementary and Alternative medicines 14, no. 5 (October 1, 2017): 80–88. http://dx.doi.org/10.21010/ajtcam.v14i5.11.

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38

MP, Tshisikhawe, NM Siaga, and RB Bhat. "Population dynamics of Millettia stuhlmannii Taub. in Ha-Makhuvha, Vhembe district of Limpopo Province, South Africa." Phyton 80, no. 1 (2011): 127–32. http://dx.doi.org/10.32604/phyton.2011.80.127.

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MP, Tshisikhawe, O. Baloyi, MH Ligavha-Mbelengwa, and RB Bhat. "The population ecology of Securidaca longepedunculata Fresen. in the Nylsvley Nature Reserve, Limpopo Province, South Africa." Phyton 81, no. 1 (2012): 107–12. http://dx.doi.org/10.32604/phyton.2012.81.107.

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40

Ruwanza, Sheunesu. "Effects of Lantana camara invasion on vegetation diversity and composition in the Vhembe Biosphere Reserve, Limpopo Province of South Africa." Scientific African 10 (November 2020): e00610. http://dx.doi.org/10.1016/j.sciaf.2020.e00610.

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41

Sebola, R. J., and K. Balkwill. "Reappraisal and identification of Olinia rochetiana (Oliniaceae) in South Africa." Bothalia 36, no. 1 (August 20, 2006): 91–99. http://dx.doi.org/10.4102/abc.v36i1.348.

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A numerical phenetic analysis of data obtained from populations of the Olinia rochetiana A.Juss. complex occurring in South Africa (Mpumalanga and Limpopo Provinces) revealed the existence of two forms: 1, a shrubby form (up to 2.5 m tall), with thick terminal branches, coriaceous leaves with a tinge of red on margins (towards the apices),short inflorescence axes, peduncles and deeply red pedicels and floral tubes/hypanthia: and 2, a slender tree form, measuring more than 4 m tall with slender terminal branches, glossy and slightly thin, papery leaves, margin colour the same as the entire lamina, and the inflor­escence axes, peduncles, pedicels and hypanthia pale green to creamy white. Differences in floral features between the twoforms correlate with differences observed in vegetative features. The two forms occupy distinct ecological niches and show tolerances and preferences for different environmental conditions such as soil type,elevation and humidity. An identificationkey for the two forms is presented.
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42

Tiawoun Makuete, A. P., M. H. Ligavha-Mbelengwa, and M. P. Tshisikhawe. "Reproductive biology towards the conservation of Securidaca longepedunculata Fresen. in the Nylsvley Nature Reserve, Limpopo Province, South Africa." South African Journal of Botany 103 (March 2016): 351. http://dx.doi.org/10.1016/j.sajb.2016.02.179.

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43

Mulabisana, M. J., M. Cloete, K. G. Mabasa, S. M. Laurie, D. Oelofse, L. L. Esterhuizen, and M. E. C. Rey. "Surveys in the Gauteng, Limpopo and Mpumalanga provinces of South Africa reveal novel isolates of sweet potato viruses." South African Journal of Botany 114 (January 2018): 280–94. http://dx.doi.org/10.1016/j.sajb.2017.11.022.

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Mnguni, Simphiwe. "Seasonal Population Abundance of Bactrocera Dorsalis Hendel (Diptera: Tephritidae) in Selected Districts of Northern KwaZulu Natal, South Africa." Journal of Environmental and Agricultural Studies 2, no. 1 (June 7, 2021): 79–84. http://dx.doi.org/10.32996/jeas.2021.2.1.8.

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The fruitfly Bactrocera dorsalis is an economically important pest that requires management for the sustainability of agriculture in South Africa. This pest has spread from the country's Northern parts within a decade, where it has completely established and spread to other neighbouring provinces. The pest spreads at various rates due to different factors given biotic and abiotic factors. Understanding factors that influence pest prevalence will assist with practical management strategies. Temperature is one of the factors that assist the invasive potential of B. dorsalis. KwaZulu Natal province is among the hotspot provinces in the country besides Limpopo and Mpumalanga. Trap catches in Northern KwaZulu Natal, uMkhanyakude and King Cetshwayo districts show that the seasonal populations of B. dorsalis are continuously present all year round, even with the application of management options to reduce pest populations. Host availability plays a significant role in the invasion and total outcompeting of other native fruitflies e.g. Ceratitis punctata.
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Jacobs, L. E. O., D. M. Richardson, and J. R. U. Wilson. "Melaleuca parvistaminea Byrnes (Myrtaceae) in South Africa: Invasion risk and feasibility of eradication." South African Journal of Botany 94 (September 2014): 24–32. http://dx.doi.org/10.1016/j.sajb.2014.05.002.

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46

Rampedi, Isaac T., and Jana Olivier. "Traditional Beverages Derived from Wild Food Plant Species in the Vhembe District, Limpopo Province in South Africa." Ecology of Food and Nutrition 52, no. 3 (May 2013): 203–22. http://dx.doi.org/10.1080/03670244.2012.706131.

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Rankoana, Sejabaledi Agnes. "Curative care through administration of plant-derived medicines in Sekhukhune district municipality of Limpopo province, South Africa." African Journal of Traditional, Complementary and Alternative Medicines 13, no. 2 (February 19, 2016): 47. http://dx.doi.org/10.4314/ajtcam.v13i2.6.

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48

Serfontein, J. J. "Xanthomonas Blight of Onion in South Africa." Plant Disease 85, no. 4 (April 2001): 442. http://dx.doi.org/10.1094/pdis.2001.85.4.442a.

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During April 1999, a foliar blight of onion (Allium cepa L. ‘Granex 33’) was reported in an early commercial planting under center pivot irrigation in the Limpopo Valley of the Northern Province of South Africa. Regular fungicide sprays failed to inhibit the progress of the disease. Foliar symptoms started as water-soaked lesions that elongated and turned chlorotic followed by tissue collapse in some leaves. Leaves often collapsed at the point of infection. Bulb size was severely reduced and premature leaf death caused irregular maturation and bulb size in the field. The symptoms were similar to those of Xanthomonas blight, described on the same cultivar in Hawaii (1). Microscopic examination of hand cut sections trough lesion margins showed bacterial streaming. Isolation on semi-selective diagnostic milk Tween agar (2) yielded almost pure cultures of a typical xanthomonad. The mucoid, yellow pigmented bacterium was rod shaped, gram negative, catalase positive, oxidase negative, utilized glucose oxidatively, and was lypolytic (Tween 80), proteolytic (skimmed milk), and amolytic. Biolog GN Microplate profiles as read by the MicroLog database release 3.50 (Biolog, Hayward, CA) were similar to those of a pathovar (similarity indices of 0.29 to 0.71). Symptoms were successfully reproduced on glasshouse grown Granex 33 seedlings at the five-leaf stage by spray and syringe inoculations (1) and the pathogen reisolated as described above. Ten seedlings were used in the pathogenicity test, of which five served as controls. After inoculation, seedlings were covered overnight with plastic bags, after which bags were removed and seedlings grown in the greenhouse at 24 to 30°C and natural light until symptom development. Attempts to isolate the pathogen from the seed lot used to plant the affected field were unsuccessful. The disease re-occurred in early plantings of Granex 33 on the same farm in April 2000 toward the end of an unusually wet summer rainy season. Damage caused by the disease was so severe in one early planting that it was plowed under. High temperatures and humid conditions combined with overhead irrigation could have enhanced disease development and spread during the early growth of the crop. No further spread was observed during cooler and drier weather later in the season. References: (1) A. M. Alvarez et al. Phytopathology 68:1132, 1978. (2) T. Goszczynska and J. J. Serfontein. J. Microbiol. Methods 32:65, 1998.
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Carmichael, D. J., M. E. C. Rey, S. Naidoo, G. Cook, and S. W. van Heerden. "First Report of Pepino mosaic virus Infecting Tomato in South Africa." Plant Disease 95, no. 6 (June 2011): 767. http://dx.doi.org/10.1094/pdis-01-11-0036.

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Pepino mosaic virus (PepMV) (genus Potexvirus) is a highly infectious virus that is responsible for significant losses in yield of tomato fruit (Solanum lycopersicum) across Europe, Asia, and the Americas in the last decade (1). During the winter growing season of 2008, uneven discoloration of tomato fruit from farms in Limpopo Province, South Africa, was detected at the Pretoria fresh produce market. Twenty fruit were randomly selected from five different suppliers in this region and the 100 samples were batched into subsamples of five fruit. Leaves with suspect mosaic and bubbling symptoms were also detected from farms in Limpopo and were thus sampled. Leaf and fruit samples were tested by double antibody-sandwich (DAS)-ELISA (2) using polyclonal antibodies against PepMV (Agdia, Elkhart, IN) combined with appropriate positive and negative controls. Fruit samples from two of the suppliers, and all leaf samples tested, reacted strongly with PepMV antibodies. Inoculum was prepared from pooled DAS-ELISA-positive leaf samples and inoculated onto 10, 4-week-old, susceptible S. lycopersicum cv. Rooikhaki seedlings. After 3 weeks, all inoculated plants had developed characteristic PepMV symptoms (2) including leaf bubbling, distortion, and curled leaves. Older leaves developed yellow spots and light/dark green leaf mosaic while apical regions were stunted and branches were distorted to form ‘nettle-head’ symptoms. Fruit surfaces were marbled or displayed flaming and uneven discoloration. Leaves from symptomatic plants were sampled for confirmation of PepMV infection by DAS-ELISA and all samples reacted positively with PepMV antibodies. Total RNA was extracted from 500-μg replicates of pooled leaf samples from infected plants with the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany), and amplified by conventional two-step reverse-transcription-PCR using a PepMV-specific primer set: Ker 1 (2) and PepCP-R (4) for a 986-bp region, including the coat protein, of the PepMV genome. PCR products were cloned into pTZ57R/T vector (Fermentas, Vilnius, Lithuania [UAB]) and six clones were purified and sequenced using universal M13 primers (3). Phylogenetic analysis clustered the sequence with EU (European), LP (Peruvian), US1 (United States)/CH1 (Chilean) and US2/CH2 PepMV isolates. The PepMV isolate accessions for US2/CH2 (AY509927, FJ612601, EF408821, FJ212288, and DQ000985) were identified as the closest relatives based on 98 to 99% nucleotide similarity obtained using BLASTN. The coat protein sequence of the South African isolate was submitted to GenBank (Accession No. HQ872607). To our knowledge, this is the first confirmed report of PepMV in South Africa. Further studies are necessary to determine its incidence and spread in this country. The presence of PepMV signals the urgent need for adoption of appropriate phytosanitary measures to restrict the spread and impact of this virus. References: (1) I. M. Hanssen and B. P. H. J. Thomma. Mol. Plant Pathol. 11:179, 2010. (2) I. M. Hanssen et al. Plant Pathol. 58:450, 2009. (3) J. Messing. Method Enzymol. 101:20, 1983. (4) I. Pagán et al. Phytopathology 96:274, 2006.
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Gololo, S. S., N. S. Mapfumari, and M. A. Mogale. "COMPARATIVE QUANTITATIVE PHYTOCHEMICAL ANALYSIS OF THE LEAVES OF SENNA ITALICA COLLECTED FROM DIFFERENT AREAS IN LIMPOPO PROVINCE, SOUTH AFRICA." International Journal of Pharmacy and Pharmaceutical Sciences 10, no. 2 (February 1, 2018): 67. http://dx.doi.org/10.22159/ijpps.2018v10i2.22950.

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Objective: The current study was aimed at comparative quantitative phytochemical analysis of the leaves of S. italica collected from four districts in Limpopo province, South Africa, in order to establish whether geographical location have an effect on the accumulation of phytochemicals within the leaves of the plant species under study.Methods: The leaves of S. italica were collected from four districts in Limpopo province of South Africa namely; Capricorn, Sekhukhune, Vhembe and Waterberg districts, dried, ground to powder and extracted using different organic solvents. The extracts of the leaf samples from different locations were subjected to quantitative phytochemical analysis for total phenolic content, total tannin content, total flavonoid content and total saponin content using spectrophotometric measurements. The resultant quantities were analysed for statistical differences.Results: The leaf samples of S. italica from the four districts in Limpopo province showed significant differences (*p<0.05) in their phytochemical quantities, with main data expressed as mean±SD. Total phenolic content was in highest amounts in leaf samples from Waterberg district compared to samples from other districts. Total tannin content was in highest amounts in the Vhembe district leaf samples compared to samples from other districts. Total flavonoid content was in highest amounts in the leaf samples from Waterberg district compared to samples from other districts. Total saponin content was in highest amounts in the Vhembe district leaf samples compared to samples from other districts.Conclusion: The findings of the study thus suggest that geographical location has an effect on the accumulation of phytochemicals in the leaves of S. italica.
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