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McCann, Maureen C. "Architecture of the plant extracellular matrix." Thesis, University of East Anglia, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.279709.
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Phelan, Thomas Joseph. "GENETIC AND MOLECULAR ANALYSIS OF PLANT NUCLEAR MATRIX PROTEINS." NCSU, 2001. http://www.lib.ncsu.edu/theses/available/etd-20011104-233111.
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PHELAN, THOMAS JOSEPH, Genetic and Molecular Analysis of Plant Nuclear Matrix Proteins. (Under the direction of Steven L. Spiker.)The eukaryotic nucleus is composed of DNA, RNA and protein, encapsulated by a nuclear envelope. DNA is compacted up to ten thousand times in order to be packaged into the nucleus. The nucleus must maintain order in the presence of a very high density and variety of protein and RNA. The nuclear matrix is a proteinaceous network thought to provide structure and organization to the nucleus. We believe that relatively stable interactions of nuclear molecules with the nuclear matrix are key to organization of the nucleus. Numerous "Matrix Attachment Region" DNA elements (MARs), have been isolated from plants, animals, and fungi. Evidence suggests that these MARs attach to the nuclear matrix, delimiting loops of chromosomal DNA. In studies of transgenic plants and animals, MARs have been shown to give important advantages to organisms transformed with genes flanked by these elements. Unlike most DNA elements, no specific sequence elements have been identified in MAR DNAs. Partly due to the insolubility of the matrix, and to the heterogeneity of MAR DNA, very few of the protein components of the nuclear matrix have been identified. This work presents analysis the proteins of the plant nuclear matrix. We have characterized a set of related proteins from the model plant Arabidopsis that associate with MAR DNA in vitro. These proteins appear to be similar to the NOP56/NOP58 family of proteins previously identified in several eukaryotic organisms. The NOP56/NOP58 proteins are thought to be involved in modifications of ribosomal RNA. Binding studies presented in this work suggest that these plant proteins may participate in RNA/DNA/protein complexes in the nucleus.
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Hohnová, Barbora. "Studium přírodních látek obsažených ve vybraných bylinách a méně obvyklých druzích drobného ovoce." Doctoral thesis, Vysoké učení technické v Brně. Fakulta chemická, 2010. http://www.nusl.cz/ntk/nusl-233303.
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Flavonoids are natural compounds widely distributed in plant kingdom. They are inseparable from human diet because they showed a protective effect against cancer, stroke and coronary heart diseases related to their antioxidant properties. Therefore, rapid and efficient extraction procedure prior to chromatographic analysis is required. The liquid extraction at elevated temperature and pressure – Pressurized fluid extraction (PFE) and Pressurized hot water extraction (PHWE), present fast, effective and environmentally friendly extraction methods for the determination of flavonoids in plant materials. PFE and PHWE followed by reversed phase high-performance liquid chromatography with UV-visible detection have been utilized for the determination of a group of flavonoids (rutin, myricetin, quercetin, luteolin, apigenin and kaempferol) in the leaves and berries of less common plants. The matrices were extracted by methanol, ethanol and water at higher temperature 40-120 oC and pressure 15 MPa during 15 minutes. The obtained results were compared with conventional Soxhlet extraction and ultrasound-assisted extraction, the same solvents were used. The most effective extraction of selected flavonoids was achieved by PHWE. PFE showed the extraction yields comparable to those of the Soxhlet extraction, and the lowest extraction power was displayed by ultrasound-assisted extraction.
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Zhao, Puyan [Verfasser]. "Arabidopsis thaliana matrix metalloproteinases (MMPs) in plant defense against pathogens / Puyan Zhao." Gießen : Universitätsbibliothek, 2012. http://d-nb.info/1064838278/34.
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Duncan, Allison B. "Relationship between remnant size and plant species richness in the Tucson urban matrix." Thesis, The University of Arizona, 2002. http://hdl.handle.net/10150/278795.
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The Sonoran Desert surrounding Tucson, Arizona is the dominant matrix in a region undergoing a transition from desert matrix to urban matrix with little emphasis placed on preserving this native ecosystem intact. Instead, patches of desert, remnants, are cut off the desert matrix and surrounded by a variety of land uses including residential, transit, and commercial. 31 sites within the City of Tucson were surveyed and the site's plant species richness, woody cover, herbaceous cover, and disturbance percentage measured. The plants found on-site were classified into native or exotic, annual or perennial, and woody or herbaceous, and further broken down into growth form. Results indicated a significant correlation between a site's area and its percent disturbance, as well as correlations between its native vegetation and area.
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MOSER, ADRIANE. "ESTIMATING HISTORICAL TRICHLOROETHYLENE EXPOSURE IN A URANIUM ENRICHMENT, GASEOUS DIFFUSION PLANT." University of Cincinnati / OhioLINK, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1121362546.
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Holmes-Davis, Rachel. "The effects of matrix attachment regions on transgene expression in Arabidopsis /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/15491.
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Zhang, Fei [Verfasser]. "Functional analysis of Arabidopsis thaliana matrix metalloproteinases and MORC in plant immunity / Fei Zhang." Gießen : Universitätsbibliothek, 2016. http://d-nb.info/1110616457/34.
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Davies, Huw Alun. "A family of glycoproteins from the petioles of Brassica campestris with potential roles in plant development and stress responses." Thesis, University of East Anglia, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317996.
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Puentes, Adriana. "Plant-Herbivore Interactions and Evolutionary Potential of Natural Arabidopsis lyrata Populations." Doctoral thesis, Uppsala universitet, Växtekologi och evolution, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-183433.
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In this thesis, I combined field, greenhouse and common-garden experiments to examine the ecological and evolutionary consequences of plant-herbivore interactions and the genetic architecture of fitness-related traits in the insect-pollinated, self-incompatible, perennial herb Arabidopsis lyrata. More specifically, I examined (1) whether damage to leaves and inflorescences affects plant fitness non-additively, (2) whether trichome production is associated with a cost in terms of reduced tolerance to leaf and inflorescence damage, (3) whether young plant resistance to a specialist insect herbivore varies among populations, and (4) whether the evolution of flowering time, floral display and rosette size is constrained by lack of genetic variation or by genetic correlations among traits. A two-year field experiment in a Swedish population showed that damage to rosette leaves and to inflorescences can affect both current and future plant performance of A. lyrata, and that effects on some fitness components are non-additive. A two-year field experiment in another Swedish population indicated that trichome-producing plants are not less tolerant than glabrous plants to leaf and inflorescence damage. In a greenhouse experiment, acceptability of young plants (5-6 weeks old) to ovipositing females and damage received by Plutella xylostella larvae varied considerably among twelve A. lyrata populations. Both oviposition and leaf damage were positively correlated with rosette size, but trichome density in the trichome-producing morph was apparently too low at this developmental stage to influence resistance to P. xylostella. In a common-garden experiment, flowering time, floral display and rosette size varied among four Scandinavian A. lyrata populations, and displayed significant additive genetic variation in some populations. Yet, strong genetic correlations between flowering start and number of flowers, and between petal length and petal width suggest that these traits may not evolve independently. Taken together, the results indicate the need to consider possible long-term and non-additive effects of herbivore damage to different plant parts, that there is no trade-off between trichome production and tolerance to herbivory, that the importance of morphological defenses against herbivory may change through plant ontogeny, and that considerable genetic variation for traits such as flowering time and floral display can be maintained in natural plant populations.
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Ward, Kathryn M. "MATRIX GEOCHEMISTRY AND PHYTOPHTHORA OCCURRENCE ON REFORESTED MINE LANDS IN APPALACHIA." UKnowledge, 2009. http://uknowledge.uky.edu/gradschool_theses/587.
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At the Bent Mountain surface mine, Pike County, Kentucky, a study has been ongoing since 2005 to assess the influence of various types of loose-graded mine spoils on water quality and forest establishment. Six research plots consist of two replicates of brown weathered sandstone, gray unweathered sandstone, and mixed brown sandstone, gray sandstone, and shale that were emplaced according to Forestry Reclamation Approach criteria. A series of analyses was initiated in 2007 to examine influence of spoil matrix composition on sulfate and carbonate geochemistry of infiltrated waters, as well as to investigate the occurrence of Phytophthora, a group of exotic forest pathogens that cause dieback and may affect success of founder species of hybrid American Chestnut (Castanea dentata Marsh.) Borkh.)), on the surface of mine spoils and in infiltrated waters.
To identify the constituent responsible for elevated sulfate concentrations, as well as determine the role of alkalinity in spoil waters, PHREEQC geochemical modeling was used to analyze aqueous speciation of sulfates and carbonates with respect to time and tailing media. Variance of δ34S values was analyzed to determine source of sulfate minerals. Oxidation of minor amounts of pyritic coal enriched the δ34S value in the brown plots. Overall, plots had acceptable water quality parameters, substantiating various types of mine spoils for the FRA, although brown proved best for tree establishment.
Methods were employed to determine whether Phytophthora were present in the surface of mine spoils and in infiltrated waters of 2005 plots as well as 2007 plots to determine if a chronosequential effect occurred. P. cryptogea was detected from surface spoil and from waters infiltrating brown sandstone plots. The brown spoil, relative to other spoil types, has greater soil moisture, greater nutrient availability, and lower pH, which may promote the occurrence and survival of the microorganism. The occurrence of the pathogen in the 2005 plots versus 2007 plots is notable; greater ground cover from colonizing species may be a precursor to Phytophthora detection on the plots. P. cryptogea is a possible threat to American chestnut, however, high infiltration rates in loose-dumped mine spoils should reduce damage by the pathogen.
Over time, the relation between water quality parameters (as influenced by spoil matrix composition), tree success, and presence of Phytophthora, is of interest as certain hydrogeochemical parameters may cause stress on trees that may increase susceptibility of plants to disease caused by Phytophthora. Alternatively, certain water geochemical parameters may directly affect Phytophthora by promoting or inhibiting survival and transport of the pathogen in spoil and infiltrated spoil waters; this too has consequences for tree establishment on loose-dumped mine spoils.
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Catzim, Divan. "Distribution of ground-layer plant species in a fragmented landscape in the Corozal District, Belize, Central America." Wright State University / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=wright1187444382.
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Clement, John Anthony. "Attachment and extracellular matrix production during the pre-invasion phase of urediniospores of the broad bean rust, Uromyces viciae-fabae." Thesis, University of Bristol, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261275.
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Anderson, Gavin Lloyd Franklin. "NUCLEAR IMPORT AND INTERACTIONS OF POTATO YELLOW DWARF VIRUS NUCLEOCAPSID, MATRIX, AND PHOSPHOPROTEIN." UKnowledge, 2014. http://uknowledge.uky.edu/plantpath_etds/12.
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Potato yellow dwarf virus (PYDV) is the type species of the genus Nucleorhabdovirus and, like all members of this genus, replication and morphogenesis occurs inside the nuclei of infected cells. Protein localization prediction algorithms failed to identify a nuclear localization signal (NLS) in PYDV nucleocapsid (N) protein, although PYDV-N has been shown to localize exclusively to the nucleus when expressed as a green fluorescent protein (GFP):N fusion in plant cells. Deletion analysis and alanine-scanning mutagenesis identified two amino acid motifs, 419QKR421 and 432KR433, that were shown to be essential for nuclear import and interaction with importin-α. Additional bimolecular fluorescence complementation showed that the PYDV-N-NLS mutants cannot be ferried into the nucleus via interaction with PYDV-P or-M. In contrast, interaction with N-NLS mutants appeared to retard the nuclear import of PYDV-P. Taken together, it was determined that PYDV-N contains the bipartite NLS 419QKRANEEAPPAAQKR433. Similarly, alanine-scanning mutagenesis was performed to determine the regions responsible for the nuclear import of PYDV-M and -P. A non-canonical NLS was identified in PYDV-P, consisting of three regions in the N-terminus of the protein required for nuclear import. PYDV-P does not interact with any Nicotiana benthamiana importins, but was found to interact with importin-α7 and -α9 of the non-host plant Arabidopsis thaliana. Two amino acids of PYDV-M, 225KR226, were found to be critical for nuclear import and interaction with importin-α. In addition, site-directed mutagenesis identified that amino acids 223LL224 of PYDV-M, which are adjacent to the two amino acids identified as responsible for nuclear import, are critical for inducing invaginations of the inner nuclear membrane. Bimolecular fluorescence complementation (BiFC) was then used to identify any differences in localization and interaction caused by the mutations introduced to PYDV-P and -M. The PYDV-P and -M proteins were still able to interact with other PYDV proteins, although the localization of the interaction differs between mutants.
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Downie, Andrew John. "The effect of cell wall matrix-modifying enzymes and other agents on the mechanical properties of plant tissue." Thesis, University of Stirling, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244694.
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Jang, Chanyong. "STRAIN-SPECIFIC PROTEIN INTERACTION AND LOCALIZATION OF TWO STRAINS OF POTATO YELLOW DWARF VIRUS AND FUNCTIONAL DOMAINS OF THEIR MATRIX PROTEIN." UKnowledge, 2019. https://uknowledge.uky.edu/plantpath_etds/25.
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Potato yellow dwarf virus (PYDV) is the type species of the genus nucleorhabdovirus which is typified by its nucleotropic characters of the members. The virus accomplishes its replication and morphogenesis in the nuclei of infected cells. Two strains, Constricta strain (CYDV) and Sanguinolenta strain (SYDV) have been described at the level of vector-specificity. CYDV is vectored by Agallia constricta and SYDV is transmitted by Aceratagllia sanguinolenta. The full-length genome of CYDV was sequenced. The 12,792 nt antisense genome encodes seven open reading frames in the order of, nucleocapsid protein (N), unknown protein (X), phosphoprotein (P), movement protein (Y), matrix protein (M), glycoprotein (G), and large polymerase protein (L). The features of each protein including a nuclear localization signal, isoelectric point, and transmembrane domain, were determined by predictive algorithms. The gene coding region was flanked by leader and trailer, and each ORF was separated by a conserved intergenic junction. In the intergenic junctions, the highly conserved cis-regulatory elements, polyadenylation signal, gene spacer, and transcription start site, were identified. The similarities of amino acid sequences between each cognate protein of SYDV and CYDV were higher than 80% except for X and P proteins. The protein localization and interaction assays of each CYDV protein identified strain-specific associations in comparison with those of SYDV and generated unique protein interaction and localization map compared to SYDV. Phylogenetic analysis using L protein identified that CYDV forms a clade with other leafhopper-transmitted rhabdoviruses. Protein sequence comparisons revealed that CYDV X has greater similarity to the cognate protein of Eggplant mottle disease virus than to SYDV X. The localization patterns of CYDV-N and -Y were different compared the cognate proteins of SYDV. The functional nuclear export domain of SYDV M was identified using c-terminal fragments of the Mwt(aa 211-243), MLL223AA(aa 211-243), and MKR225AA(aa 211-243). Based on the data, the functional domains M mediating membrane association, nuclear import and export were mapped for both strains and suggested a model whereby M mediates intra- and intercellular movement of PYDV nucleocapsid.
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Freitas, Edjane Gonçalves de. "Uso de informações de parentesco e modelos mistos para avaliação e seleção de genótipos de cana-de-açúcar." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/11/11137/tde-30102013-112348/.
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Nos programas de melhoramento de cana-de-açúcar todos os anos são instalados experimentos com o objetivo de avaliar genótipos que podem eventualmente ser recomendados para o plantio, ou mesmo como genitores. Este objetivo é atingido com o emprego de experimentos em diferentes locais, durante diferentes colheitas. Além disso, frequentemente há grande desbalanceamento, pois nem todos os genótipos são avaliados em todos os experimentos. O emprego de abordagens tradicionais como análise de variância conjunta (ANAVA) é inviável devido à condição de desbalanceamento e ao fato de as pressuposições não modelarem adequadamente o relacionamento entre as observações. O emprego de modelos misto utilizando a metodologia REML/BLUP é uma alternativa para análise desses experimentos em cana-deaçúcar, permitindo também incorporar a informação de parentesco entre os indivíduos. Nesse contexto, foram analisados 44 experimentos (locais) de cana-de-açúcar do programa de melhoramento da cana-de-açúcar do Instituto Agronômico de Campinas (IAC), com 74 genótipos (clones e variedades) e com até 5 colheitas. O delineamento foi o de blocos ao acaso com 2 a 6 repetições. O caráter analisado foi TPH (Tonelada de pol por hectare). Foram testados 40 modelos, os 20 primeiros foram avaliadas diferentes estrutura de VCOV para locais e colheitas, e os 20 seguintes, além das matrizes de VCOV, foi incorporada a matriz de parentesco genético, A. De acordo com AIC, verificou-se que o Modelo 11, o qual assume as matrizes FA1, AR1 e ID, para locais, colheitas e genótipos, respectivamente, foi o melhor, e portanto, o mais eficiente para seleção de genótipos superiores. Quando comparado ao modelo tradicional (médias dos experimentos), houve mudanças no ranqueamento dos genótipos. Há correlação entre o modelo tradicional e o Modelo 11 (_ = 0, 63, p-valor < 0, 001). A opção de utilizar modelo misto sem ajustar as matrizes de VCOV (Modelo 1) é relativamente melhor do que usar o Modelo Tradicional. Isto foi evidenciado pela correlação mais alta entre os modelos 1 e 11 (_ = 0, 87 com p-valor < 0, 001). Acredita-se que o emprego do Modelo 11 junto com experiência do melhorista poderá aumentar a eficiência de seleção em programas de melhoramento de cana-de-açúcar.In breeding programs of sugarcane every year experiments are installed to evaluate the performance of genotypes, in order to select superior varieties and genitors. The use of ordinary approaches such as joint analysis of variance (ANOVA) is unfeasible due to unbalancing and assumptions that do not reflect the standard of relationship of the observations. The use of mixed models using the method REML/BLUP is an alternative. It also allows the incorporation of information from kinship between individuals. In this context, we analyzed 44 trials (locations) of sugarcane breeding program of sugarcane (Agronomic Institute Campinas, IAC), with 74 genotypes (varieties and clones), up to 5 harvests. The experimental design was randomized blocks with 2-6 replicates. The character was examined TPH (Tons of pol per hectare). We tested 40 models, the first 20 were evaluated different VCOV structure to locations and harvests, and 20 following addition of matrix VCOV was incorporated genetic relationship matrix, A. Under AIC, it was found that the model 11, which assumes matrices FA1, AR1 and ID for locations, harvests and genotypes, respectively, was the best. There is a moderate correlation between traditional model and model 11 (_ = 0.63, p-value < 0.001), when ranking the genotypes. The option of using mixed model without adjusting matrices VCOV (model 1) is better than using the traditional model. This was suggested by the higher correlation between models 1 and 11 (_ = 0.87 with p-value < 0.001). We believe that the usage of model 11 together with breeders experience can increase the efficiency of selection in sugarcane breeding programs.
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Almeida, Ana Patrícia da Silva. "Dispersão de sementes por aves na galeria ripícola e matriz envolvente." Master's thesis, Universidade de Évora, 2016. http://hdl.handle.net/10174/18611.
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As galerias ripícolas são habitats com uma grande disponibilidade de recursos alimentares e abrigo para a fauna, estando entre os habitats mais importantes e vulneráveis do mundo. São uma fonte de produção de frutos, o que atrai muitos animais, como as aves frugívoras, que são os principais dispersores de sementes na bacia do Mediterrâneo. O objetivo principal deste estudo foi estudar os padrões de dispersão de sementes por aves na interface galeria ripícola-matriz florestal envolvente (Montado). Foram marcadas com fluorescência plantas-mãe produtoras de frutos, e utilizadas armadilhas para sementes e transectos, para a recolha dos dejetos de aves onde, posteriormente, se detetaram as sementes dispersadas. Constatou-se que a dispersão de sementes e a abundância de plantas-mãe foi superior a distâncias mais próximas da ribeira, diminuindo à medida que esta distância aumenta. A espécie mais dispersada foi Smilax aspera e as espécies com sementes de menor dimensão, foram dispersadas a uma maior distância da planta-mãe, provavelmente devido a este tipo de sementes permanecerem mais tempo no trato intestinal das aves. Os resultados deste estudo apresentam algumas implicações para a colonização da matriz adjacente por plantas produtoras de frutos; ABSTRACT: Riparian galleries are habitats with a large availability food resources and shelter for animals, being among the most important and vulnerable habitats in the world. These zones are a great source of fruits attracting many animals, such as frugivorous birds, which are the main seed dispersers in the Mediterranean basin. The objective of this thesis is the study of seed dispersal patterns by birds, in the riparian gallery and in the surrounding forest matrix (Montado). Fruits of several plant species were used to collect bird droppings and detect fluorescent seeds in those droppings. Fruit abundance and seed dispersal were higher close to the stream, and decreased rapidly with an increase in distance from the stream. From all species, the most dispersed was Smilax aspera and species with smaller seed were found at a significantly larger distance from parents plants than species with large seeds. This can be explained by the fact that smaller seed stay longer in the intestinal tract of the birds. The results of this study present some implications for the colonization of freshly fruit plants from the riparian gallery into the adjacent matrix.
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Shahani, Priya. "Demography and native bee pollination of Sedum laxum (Crassulaceae), an endemic, clonal plant in a managed forest matrix landscape of the Siskiyou Mountains /." Diss., Digital Dissertations Database. Restricted to UC campuses, 2007. http://uclibs.org/PID/11984.
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Ndimba, Bongani Kaiser. "Identification and characterisation of the Arabidopsis thaliana cell wall proteome : unravelling novel cell wall proteins and new potential functions of the plant extracellular matrix." Thesis, Durham University, 2001. http://etheses.dur.ac.uk/3858/.
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The application of the proteomic approach has facilitated efforts directed toward the mapping of the entire Arabidopsis cell wall proteome. Proteins were sequentially extracted from purified cell walls using 0.2 M CaC1(_2) followed by a urea buffer. The extracts were resolved via large format two dimensional polyacrylamide gel electrophoresis (2-D PAGE) and were visualised via Coomassie brilliant blue staining. The aim was to identify and characterise as many cell wall proteins as possible, with the hope of identifying novel cell wall proteins. Out of 325 spots visualised on the 2-D polyacrylamide gel, 144 gave a positive protein identification representing 104 different proteins. The identified proteins were divided into 3 categories. The first category included proteins that have been previously identified as plant cell wall proteins. The second category was designated to include novel cell wall proteins (hypothetical proteins) and the third category was made up of proteins, which had recognised functions, but had never hitherto been known to be secreted to the extracellular matrix. Among the identified novel cell wall proteins there were several that shared high homology with protein kinases. These proteins possessed all the characteristics of secreted polypeptides, such as the cleavable N-terminal signal peptide, and were found to lack both the transmembrane domain and the endoplasmic reticulum retention tetrapeptides (HDEL and KDEL). These observations suggested that, as in animal cells, plant cells had extracellular protein kinase activity (phosphorylation). This was supported by the recent discovery that plant cells secrete ATP to the extracellular matrix (Thomas et al., 2000). Verification of the occurrence of extracellular protein kinase activity was further strengthened by the identification of phosphorylated bona fide cell wall proteins and stress responses caused by the depletion extracellular ATP.
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Løe, Geir. "Ecology and Evolution of Resistance to Herbivory : Trichome Production in Arabidopsis lyrata." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6359.
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Oliveira, Leandro Silva de. "Propagação de Eucalyptus cloeziana F. Muell." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/11/11150/tde-05052014-154718/.
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O Eucalyptus cloeziana se destaca pelas propriedades tecnológicas da sua madeira, principalmente em razão da sua durabilidade, densidade e resistência. Entretanto, essa espécie apresenta limitações quanto ao enraizamento adventício de estacas, dificultando a obtenção de mudas clonais e o avanço nos programa de melhoramento da espécie. Nesta perspectiva, o presente trabalho teve por objetivo avaliar a micropropagação de E. cloeziana a partir de materiais juvenis e adultos como uma técnica para a propagação da espécie. Para tanto, o trabalho foi dividido em quatro partes. No primeiro estudo, estabeleceu-se um protocolo de organogênese indireta de E. cloeziana a partir de hipocótilos e cotilédones. No segundo estudo avaliou-se o resgate de matrizes adultas de E. cloeziana através da indução de brotações epicórmicas em megaestacas. No terceiro estudo definiu-se um protocolo de micropropagação via proliferação de gemas axilares de matrizes adultas de E. cloeziana. Por último, no quarto estudo avaliou-se o rejuvenescimento in vitro de matrizes adultas de E. cloeziana por meio da micropropagação via proliferação de gemas axilares e a microestaquia, a partir das matrizes rejuvenescidas in vitro,para comprovar a viabilidade do cultivo in vitro como alternativa de propagação da espécie. A organogênese indireta de E. cloeziana mostrou-se possível e dependente do tipo de explante, dos reguladores de crescimento, suas concentrações e combinações utilizadas nas diferentes fases de morfogênese. As brotações adventícias foram micropropagadas e aclimatizadas com sucesso, obtendo-se mudas clonais de E. cloeziana. No resgate das matrizes adultas de E. cloeziana ocorreu maior indução de brotações epicórmicas para as megaestacas coletadas na época do ano com maior pluviosidade e temperatura. O diâmetro médio das megaestacas, compreendido entre 2,0 e 5,0 cm, correspondeu ao mais adequado para a obtenção de maior número de brotações epicórmicas no resgate das matrizes adultas de E. cloeziana. O protocolo de micropropagação das matrizes adultas de E. cloeziana foi estabelecido com sucesso, utilizando como explantes, brotações epicórmicas induzidas nas megaestacas. A multiplicação in vitro das microcepas foi realizada com êxito no meio de cultura WPM suplementado com BAP e ANA, cujas concentrações variaram entre os genótipos. Os tratamentos de \"pulse\" com GA3 não foi adequado para promover o alongamento in vitro das brotações, o qual foi obtido coma redução da concentração do BAP (0,1 mg L-1). A aclimatização e o enraizamento ex vitro das microestacas foi realizada com sucesso nos miniestufas, garantindo a obtenção de mudas clonais das matrizes adultas de E. cloeziana para a composição de um microjardim clonal. As microcepas das árvores matrizes de E. cloeziana apresentaram diferentes taxas de multiplicação in vitro, apresentando especificidades dos materiais genéticos à micropropagação. Os resultados da microestaquia das matrizes rejuvenescidas in vitro de E. cloeziana corroboraram as evidências de rejuvenescimento desses genótipos durante a micropropagação. Além disso, constatou-se que outros fatores, além da maturidade, podem estar atuando diretamente na recalcitrância do E. cloeziana ao enraizamento adventício. Dessa forma, comprovou-se a importância da micropropagação como uma ferramenta para a clonagem de E. cloeziana, abrindo perspectivas para investigações futuras para otimizar os métodos de propagação da espécie.Eucalyptus cloeziana has importance for its wood technologic characteristics, mainly the durability, density and resistance. Moreover, this species has limitations on adventitious rooting of cuttings, having difficulties to obtain clonal seedlings and to advance in the improvement programs. In this perspective, the present work aimed to evaluate the E. cloeziana micropropagation by juvenile and mature explants as a technique for propagation this specie. Therefore, the work was divided into four basic studies. The first study was the establishment of the protocol to indirect organogenesis to E. cloeziana hypocotyls and cotyledons. In the second study evaluated the rescue of E. cloeziana adult matrices for epicormic shoots induction in crow branches. In the third study was definite a micropropagation protocol by axillary branching of E. cloeziana adult matrices. Finally, in the fourth study was evaluated the in vitro rejuvenation of E. cloeziana adult matrices to micropropagation by axillary branching and the micro-cutting technique of in vitro rejuvenated matrices to prove the in vitro culture viability to the propagation of this specie. The indirect organogenesis E. cloeziana was dependent of explant type and growth regulator and its concentration used in the different morphogenesis phases. The adventitious shoots were multiplicities and acclimatized with success to obtain E. cloeziana clonal seedlings. The vegetative rescue results of E. cloeziana adult matrices showed a higher induction epicormic shoots from branches collected in warmer and rain season. The medium diameter between 2.0 and 5.0 cm was considered the better to obtain higher epicormic shoots number in vegetative rescue. The micropropagation protocol of E. cloeziana adult matrices was realized with success using epicormic shoots, induced in the branches, like explants. The shoots in vitro multiplication was realized on WPM medium culture, supplemented with BA and NAA. The better growth regulators concentrations were different for each genotype. Treatments pulse with GA3 was not suitable to promote the elongation of shoots in vitro, which was obtained by the BA concentration reduction at 0.1 mg L-1. The in vitro shoots acclimatization and ex vitro rooting was performed in mini-incubators with success and it permit to obtain clonal seedlings from E. cloeziana adult matrices to form a clonal micro-garden. The shoots of E. cloeziana adult matrices showed different in vitro multiplication rates with specific-genotype responses to micropropagation. The micro-cutting technique results of E. cloeziana adult matrices provide evidence that occurred in vitro rejuvenation of these genotypes, during the micropropagation. Therefore, the nursery results showed that other factors, not only maturation, can be involved in the adventitious recalcitrance of E. cloeziana. In conclusion, the importance micropropagation like a biotechnological tool to propagation of E. cloeziana was proved and opened important perspectives for future investigations to optimize the propagation methods for this specie.
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Jeong, Sun Yong. "Functional investigation of arabidopsis long coiled-coil proteins and subcellular localization of plant rangap1." The Ohio State University, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=osu1086119855.
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Niva, Mikael. "Life History Strategies in Linnaea borealis." Doctoral thesis, Uppsala : University Library : Univ.-bibl. [distributör], 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3604.
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Blahová, Eliška. "Stanovení vybraných "Musk" sloučenin v biotických vzorcích." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216382.
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Musk compounds (MUSK) or synthetic fragrances are organic substances commonly used as fragrant constituents of detergents, soaps, cosmetics, personal care products, industrial and in-house cleaning agents, industrial plasticizers, chewing tobacco and fresheners. The big attention is pushed ahead studying these compounds, their fate in different parts of ecosystems and studying their characteristics at present, because musks infiltrate many environmental components, particularly aquatic and marine ecosystems, through their large application and their ability to be perzistant. This diploma thesis was focused on four synthetic “classical” fragrances used over the world. The aim of this study was to perform a method optimisation for the determination of selected fragrances in biotic matrix. There was made an evalution of ability of selected water treatment plant to clear away musks from water, these results were used for evaluating contamination measurement of aquatic ecosystem. The identification and quantification of analytes was carried out by high resolution gas chromatography - mass spectrometry (HRGC/MS).
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Borges, Rafael Cruz. "Um algoritmo para sintonia de controladores robustos para amortecimento de modos intra-planta em sistemas de potência." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/18/18154/tde-10082009-114735/.
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O problema de modos intra-planta em sistemas de potência é caracterizado pela oscilação dos geradores de uma mesma usina uns contra os outros. Estes modos são geralmente bem amortecidos, mas uma sintonia inadequada dos parâmetros dos PSSs (Power System Stabilizers), visando fornecer um amortecimento aos modos locais, por exemplo, pode reduzir o amortecimento dos modos intra-planta. Esta possibilidade pode ser evitada por uma sintonia cuidadosa dos estabilizadores, em um modelo que seja capaz de representar os modos intra-planta. Este processo de sintonia pode ser tedioso e demorado, exigindo muitas horas de trabalho de um especialista. Neste trabalho, apresentamos um algoritmo computacional capaz de realizar esse processo, com uma intervenção mínima do projetista (reduzindo, portanto, o esforço do engenheiro projetista na sintonia do PSS e deixando que o computador faça de maneira eficiente) e aplicamos este algoritmo para encontrar uma sintonia adequada de PSSs para o amortecimento dos modos local e intra-planta coexistentes no mesmo sistema. Como mostrado neste trabalho, o algoritmo é capaz de sintonizar os PSSs para varias condições de operação de uma só vez, o que pode também resultar em uma melhor sintonia quando comparado com abordagens seqüenciais de projeto.The problem of intra-plant modes is characterized by the oscillation of the machines within a power station against each other. These modes are usually well-damped, but improper settings for the parameters for power system stabilizers (PSSs), aiming at enhancing the damping of local modes, for example, can reduce the damping of the intra-plant modes. This possibility must be avoided by a careful tuning of the stabilizers, over a model that is able to represent the intra-plant modes. This tuning process may be involved and time-consuming, requiring many hours of work from a specialist. In this paper, we present a computerized algorithm that is capable of performing this process with minimum intervention of the human designer (therefore alleviating the burden of PSS tuning for the engineer and placing most of it on the computer) and apply it to find a PSS tuning to damp both local and intra-plant modes of the same plant. As shown in the paper, the algorithm is capable of tuning PSSs for several operating conditions at once, which may also result in better tuning when compared to sequential approaches.
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Hammad, Mira. "Reconstruction of auricular cartilage using natural-derived scaffolds with an in vivo application in rabbit model Effects of hypoxia on chondrogenic differentiation of progenitor cells from different origins Cell sheets as tools for ear cartilage reconstruction in vivo Cartilage tissue engineering using apple cellulosic scaffolds Cell-secreted matrices as cell supports: Novel approaches for cell culture applications." Thesis, Normandie, 2021. http://www.theses.fr/2021NORMC404.
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La reconstruction des défauts du cartilage auriculaire nécessite une restauration appropriée par des sources cellulaires adéquates ainsi que la fourniture de supports appropriés pour les tissus. Ce travail visait à étudier différents échafaudages et biomatériaux pour l'ingénierie in vitro du cartilage auriculaire ainsi que la réparation in vivo du cartilage auriculaire chez des modèles de lapin. Nous avons d'abord montré que les périchondrocytes auriculaires sont les meilleurs candidats pour la régénération du cartilage auriculaire et que l'hypoxie n'est pas nécessaire à leur différenciation chondrogénique. Ces cellules ont formé avec succès des feuillets de cellules cartilagineuses que nous avons utilisés pour régénérer le tissu cartilagineux in vitro et pour combler et reconstruire les défauts du cartilage in vivo dans des modèles allogèniques de lapins. Nous avons ensuite testé des tissus dérivés de la cellulose en décellularisant un tissu de pomme. Une fois recolonisés avec des cellules, ces échafaudages ont surpassé les hydrogels d'alginate en augmentant la croissance et en régulant l'expression cartilagineuse dans différentes cellules de mammifères. Dans la dernière partie de la thèse, nous avons examiné des matrices sécrétées par les cellules et les avons utilisées comme revêtement pour différentes applications de culture cellulaire. Il est intéressant de noter que ces supports, une fois lyophilisés, ont favorisé la culture de cellules allo- et xénogéniques, augmenté la prolifération et stimulé la chondrogenèse. Nous avons également mis en évidence la préservation du phénotype lors de l’amplification des chondrocytes par passages successifs. Notre étude fournit de nouveaux outils et approches pour de multiples applications de culture cellulaireSuccessful reconstruction of auricular cartilage defects requires the appropriate restoration of the cartilaginous deformities by potential cell sources as well as providing suitable tissue supports. This work aimed to investigate different scaffolds and biomaterials for in vitro auricular cartilage engineering as well as in vivo auricular cartilage repair in rabbit models. We first showed that auricular perichondrocytes are the best candidates for auricular cartilage regeneration and hypoxia is not necessary for their chondrogenic differentiation. These cells successfully formed cartilaginous cell sheets which were used to regenerate cartilage tissue in vitro and to fill and reconstruct cartilage defects in vivo in allogenic rabbit models. Furthermore, we tested cellulose-derived tissue by decellularizing apple tissue and its use as a scaffold. Repopulated with cells, these scaffolds surpassed alginate hydrogels by enhancing colonization and upregulating the cartilaginous expression in different mammalian cells. In the final part of the thesis, we examined cell-secreted matrices and used them as a coating for different cell culture applications. Interestingly, these coatings promoted both allo- and xenogeneic cell culture, increased proliferation, and boosted chondrogenesis. We also highlighted phenotype preservation during chondrocytes expansion on these cell-secreted matrices. Our study provides novel tools and approaches for multiple cell culture applications
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Case, Olivia Hildegard. "An assessment of medicinal hemp plant extracts as natural antibiotic and immune modulation phytotherapies." Thesis, University of the Western Cape, 2005. http://etd.uwc.ac.za/index.php?module=etd&.
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This study aimed to evaluate the antimicrobial efficacy of medicinal hemp plant extracts to determine the antibacterial effects of indigenous Sansevieria species and exotic Cannabis sativa phytotherapy varieties. This study also assessed whether aqueous o
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Shields, C. J. "Matrix photochemistry with plane-polarised light." Thesis, University of Strathclyde, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372121.
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Kadlec, Tomáš. "Porovnání koncepcí zásobování materiálem s využitím počítačové simulace." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2021. http://www.nusl.cz/ntk/nusl-442810.
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The aim of the diploma thesis is to design of a new concept of material handling from the automatic warehouse to the manufacturing area and comparison with the original concept using computer simulation. The simulation model is created in Plant Simulation software from PLM Software. Part of the thesis is to compile a matric of experiments and perform simulation experiments in order to compare both concepts.
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Osterrieder, Anne. "Dynamics of Golgi matrix proteins in planta." Thesis, Oxford Brookes University, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490517.
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The plant Golgi apparatus plays a central role in the secretory pathway, being the site of protein glycosylation, sorting and the synthesis of cell wall polysaccharides and glycolipids. Golgi matrix proteins are involved in the formation and maintenance of the Golgi stack and putative plant homologues have been identified, but their general function and role in plant Golgi biogenesis still is unknown. To study Golgi biogenesis, the Golgi was disrupted using both the drug Brefeldin A (BFA) and a mutated GTP-Iocked form of the small GTPase Sarlp, blocking protein transport from the endoplasmic reticulum to the Golgi. As prolonged expression of Sarl-GTP is toxic to cells, the gene was inserted into a dexamethasone-inducible promoter system, allowing controlled expression and Golgi disruption. The system was characterised by live cell imaging of fluorescent Golgi and ER exit site markers and at the ultrastructural level by electron microscopy. A novel method to reverse the dexamethasone-inducib'e system in tobacco using the glucocorticoid antagonist RU486 was established. The subcellular location of the Golgi matrix proteins GFP-AtCASP, GFP-golgin-84, AtGRIP-GFP and GFP-TMF and other Golgi markers fused to fluorescent proteins was studied during Golgi disruption and reformation. After disruption of Golgi membranes, the trans-Golgi matrix proteins GFP-TMF and AtGRIP-GFP were redistributed into the cytoplasm. The cis-Golgi matrix proteins GFP-AtCASP and GFP-golgin-84 labelled not only the ER and cytoplasm respectively, but also remnant punctate structures. Those remnants were found to co-locate with the ER exit site marker Sarl-GTP-YFP, indicating a close relationship between the cis-Golgi matrix and ER exit sites. After BFA washout, cis-Golgi matrix proteins labelled the reforming Golgi before transGolgi matrix proteins. Interactions between Golgi matrix proteins and regulatory proteins were visualised in vivo in tobacco leaf epidermal cells using fluorescence lifetime Imagmg (FUM) combined with fluorescence resonance energy transfer (FRET).
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Chan, Xiao Ying. "Molecular expression of recombinant Apoptin in planta and preliminary evaluation of biological characteristics of plant-made Apoptin on cancerous cells." Thesis, University of Nottingham, 2017. http://eprints.nottingham.ac.uk/42889/.
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Apoptin, a potential anticancer candidate, selectively kills tumour or transformed cells but remains harmless to normal and non-transformed cells. Besides, apoptin-induced apoptosis is independent of p53 apoptosis pathway, which is always mutated in cancer cells during tumorigenesis or after radiotherapy. This has made apoptin becoming more pharmacological valuable. In this study, the general aim was to develop a plant-derived apoptin which offers a safer and more cost-effective treatment for cancer patients. The scope of the study focused on the production of recombinant apoptin in a plant-based system and preliminary bioactivity evaluation of the purified plant-derived apoptin on human lung cancer adenocarcinoma A549 cells. Recombinant apoptin was expressed in Nicotiana benthamiana as apoptin alone, in fusion to green fluorescent protein (GFP) as well as in fusion to lichenase (Lic) to increase the expression of recombinant protein in soluble fraction. Recombinant apoptin was also in fusion to H22 single chain antibody and epidermal growth factor (EGF) in order to target recombinant H22-apoptin and EGF-apoptin to cancer cells overexpressed with immunoglobulin G (IgG) receptor (CD64) and EGF receptors. Expression of recombinant apoptin was detected in N. benthamiana successfully, however, high amount of soluble protein was obtained in plants infiltrated with recombinant GFP-apoptin (gene casette: PR-GFP-VP3-HK) and EGF-apoptin (gene casette: PR-EGF-CatAd-VP3-HK) that targeted the recombinant proteins to endoplasmic reticulum (ER). Protein purification using immobilised metal affinity chromatography (IMAC) recovered recombinant GFP-apoptin (GFP-VP3-HK) and EGF-apoptin (EGF-CatAd-VP3-HK) at a low purity when recombinant proteins were purified in a non-denaturing condition. Host cell protein contamination was not able to be removed when second chromatography and acid precipitation method were used. However, recombinant GFP-apoptin (GFP-VP3-H) and lichenase-apoptin (Lic-VP3-H) without targeted to specific cellular compartment were purified at a good purity using IMAC. Recombinant GFP-VP3-H extracted in a denaturing condition was successfully refolded without an addition of chemical additives while recombinant Lic-VP3-H required triton to refold the protein. In cell-based study, enzyme-linked immunosorbent assay (ELISA) showed that apoptin interacted with EGF receptors as well as A549 cells which finding is the first of its kind in report but with an unverified speculation. On the other hand, nuclear localisation activity and a few apoptosis-associated morphological features were observed in cells microinjected with recombinant GFP-VP3-H. Meanwhile, recombinant EGF-VP3-HK showed a dose-dependent growth inhibitory effect at higher concentration and caused the loss of mitochondrial membrane potential (MMP) in treated A549 cells. However, internalisation of recombinant EGF-VP3-HK requires a further study for confirmation. Considering the findings on MMP and caspase 3/7 assays were not convincing enough, further evaluations are necessitated in order to verify the apoptosis events induced by the recombinant GFP-VP3-H and EGF-VP3-HK. Nonetheless, this study has heralded a new milestone for apoptin research by which its protein has been successfully produced in plants and some preliminary biological characteristics have been explored at a certain extent.
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Neto, André Pinto Raposo. "Gestão de talento em empresas de outsourcing : proposta de modelo aplicado a Contact Centers." Master's thesis, Instituto Superior de Economia e Gestão, 2020. http://hdl.handle.net/10400.5/20722.
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Mestrado em Gestão de Recursos HumanosO objetivo do presente projeto é a criação de um modelo de Gestão de Talento numa empresa de outsourcing de Contact Centers. Assim, pretende-se otimizar os recursos humanos, nomeadamente na identificação, gestão e desenvolvimento de talento entre operadores de Contact Centers e melhorar processos existentes de desenvolvimento e progressão de carreira. Recorreu-se a uma metodologia qualitativa análise documental e entrevistas semi-estruturadas e, no mapeamento do talento, elaborou-se uma matriz com dois eixos, Potencial e Desempenho, aferidos através da avaliação de desempenho e de questionários de avaliação de potencial. Considerando os quadrantes criados na matriz e algumas das necessidades verificadas através das entrevistas realizadas, foi elaborado um Plano de Ação, que sugere, entre outras medidas, ações formativas de modo a desenvolver o talento identificado e, paralelamente, aumentar os níveis de motivação entre operadores. Com este modelo pretende-se que, a médio/longo prazo, a organização diminua os níveis de rotatividade entre operadores, diminua custos inerentes ao recrutamento/seleção e posterior formação de novos operadores, conseguindo assim aumentar a retenção entre os atuais.
The purpose of this project is to create a Talent Management model in a Contact Center outsourcing company. It is intended to optimize human resources, namely in the identification, management and development of talent among Contact Center operators and to improve existing processes of development and career progression. A qualitative methodology was used, consisting of document analysis and semi-directive interviews and in the mapping of talent, a matrix with two axes, Potential and Performance, was elaborated and assessed through performance appraisal and potential evaluation questionnaires. Considering the quadrants created in the matrix and some of the needs verified through the interviews which were carried out, an Action Plan was prepared. The Action Plan suggests, among other measures, training actions in order to develop the identified talent and, in parallel, increase the motivation levels among operators. Through the application of this model, it is intended that in the medium/long term, the organization reduces the levels of turnover among operators, reduces costs inherent to recruitment / selection and subsequent training of new operators, therefore managing to increase retention among current operators.
info:eu-repo/semantics/publishedVersion
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Stott, Iain Michael. "Modelling transient population dynamics and their role in ecology and evolution." Thesis, University of Exeter, 2012. http://hdl.handle.net/10036/3733.
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Population projection matrix (PPM) models are a central tool in ecology and evolution. They are widely used for devising population management practises for conservation, pest control, and harvesting. They are frequently employed in comparative analyses that seek to explain demographic patterns in natural populations. They are also a key tool in calculating measures of fitness for evolutionary studies. Yet, demographic analyses using projection matrices have, in some ways, failed to keep up with prevailing ecological paradigms. A common focus on long-term and equilibrium dynamics when analysing projection matrix models fits better with the outmoded view of ecosystems as stable and immutable. The more current view of ecosystems as dynamic and subject to constant extrinsic disturbances has bred new theoretical advances in the study of short-term "transient" dynamics. Transient dynamics can be very different to long-term trends, and given that ecological studies are often conducted over short timescales, they may be more relevant to research. This thesis focuses on the study of transient dynamics using population projection matrix models. The first section presents theoretical, methodological and computational advances in the study of transient dynamics. These are designed to enhance the predictive power of models, whilst keeping data requirements to a minimum, and borrow from the fields of engineering and systems control. Case studies in this section provide support for consideration of transient dynamics in population management. The second section applies some of these new methods to answer pertinent questions surrounding the ecology and evolution of transient dynamics in plants. Results show that transient dynamics exhibit patterns according to life form and phylogenetic history. Evidence suggests that this can be linked to the stage-structuring of life cycles, which opens up the possibility for new avenues of research considering the evolution of transient dynamics in nature.
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Al-Maalouf, Samar Wadih. "Exploration of a mammary epithelial cell model for the study of epithelial inflammation and mechanisms of anti-inflammatory activity in medicinal plants." Columbus, Ohio : Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1166806742.
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Corrêa, Christiane Erondina. "Ecologia de populações de Psychotria nuda (Rubiaceae) em Floresta Ombrófila Densa." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/315903.
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Orientador: Flavio Antonio Maës dos SantosTese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia
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Resumo: Os habitats variam entre si quanta às características abióticas como luminosidade e topografia. A variação altitudinal pode conter essas diferenças e influenciar variações em escala individual e populacional das espécies. 0 objetivo geral desse trabalho foi descrever as características da espécie em escala do indivíduo, o padrão espacial e a dinâmica populacional do arbusto Psychotria nuda (Rubiaceae). Adicionalmente, verifica se há variação dessas características populacionais entre altitudes e dentro de cada altitude, considerando variações da topografia e luminosidade. 0 estudo foi realizado em Floresta Ombrófila Densa, no Parque Estadual da Serra do Mar em diferentes altitudes (Floresta de Restinga, Terras Baixas, Sub-Montana e Montana). Foram identificadas duas formas de crescimento, ereta e prostrada. lndivíduos prostrados podem apresentar crescimento clonal, encontrado em mais de 70% dos casas. 0 comprimento, o diâmetro, a área de copa e o módulo de elasticidade dos indivíduos eretos foram maiores comparados aos prostrados. Mais de 80% dos indivíduos amostrados ocorreram nas parcelas Terras Baixas. 0 comprimento foi semelhante entre altitudes, mas o diâmetro e a área da copa foram maiores nos indivíduos da Sub-Montana. A variação dos indivíduos localizados na Sub-Montana foi menor comparado aos indivíduos nas Terras Baixas. 0 padrão espacial diferiu entre as altitudes. Na Sub-Montana e na Restinga a distribuição foi agregada à pequenas distâncias e na Terras Baixas foi aleatória. Para todas as altitudes não houve variação temporal do padrão espacial. Mesmo avaliando a distribuição dos indivíduos por tamanho ou forma de crescimento, as variações influenciadas pela altitude foram preponderantes. A distribuição da densidade dos indivíduos não esteve relacionada a variações da topografia e luminosidade. 0 crescimento individual variou pouco ao Iongo do tempo e entre altitudes. Não houve variação da taxa de crescimento decamétrico com as classes de diâmetro dos indivíduos. As taxas de crescimento decamétrico não estavam relacionadas as variáveis abióticas. A taxa de crescimento populacional foi positiva, próxima a 1, nas duas altitudes. As taxas de mortalidade e de recrutamento foram muito semelhantes entre altitudes e não variaram temporal ou espacialmente. Indivíduos menores são mais freqüentes nas populações estudadas e a probabilidade de reprodução esteve associada ao tamanho do indivíduo. A maior proporção indivíduos reprodutivos na população foi encontrada na Sub-Montana. A maioria dos indivíduos permaneceu na classe de diâmetro inicial. Houve casos de regressão em até duas classes de diâmetro que podem significar a diminuição de tamanho ou fragmentação de rametas. O ingresso por reprodução sexuada foi pequeno sendo a maior parte dos recrutas originados por propagação vegetativa (91,6%). As duas altitudes diferiram quanto as contribuições para as diferenças encontradas no ?a, indicando que diferentes transições são importantes em cada hábitat. Algumas características populacionais estudadas variaram mais fortemente com a altitude em comparação a outros. As diferenças destacam que as populações das duas altitudes mantiveram estabilidade, entretanto a partir de estratégias diferentes. Na altitude maior os indivíduos foram maiores e apenas indivíduos grandes foram reprodutivos. Na altitude menor os indivíduos iniciam a reprodução com tamanho menor e ocorre maior entrada de indivíduos por propagação vegetativa
Abstract: The habitats may differ among themselves by abiotic traits like light and slope. The altitudinal variation may include these differences and influence changes in species on both individual and populational scales. The goals of this work were to describe individual traits, spatial pattern and population dynamic of shrub Psychotria nuda. Besides verify if there were variations of these population traits between altitudes and in each altitude considering slope and canopy openness variations. We developed this work in Dense-Ombrophylous Forest of Serra do Mar State Park in distinct altitudes. Two growth forms were recognized, erect and prostrate. The prostrate growth form includes the plants derived from clonal growth which is present in more than 70% of recorded individuals. The length, diameter, crown area and elastic modulus of erect growth form were higher than the prostrate one. More than 80% of the individuals occurred in the Lowland Secondary-Dense-Ombrophylous Forest. The length was similar between altitudes, but the diameter and crown area were higher in SM. Plants variations were smaller in Submontane Secondary-Dense-Ombrophylous Forest plots than in Lowland Secondary-Dense-Ombrophylous Forest. In Submontane Secondary Dense-Ombrophylous Forest and Coastal Plain Forest the distribution were aggregate to small distances and in Lowland Secondary-Dense-Ombrophylous Forest was random. There was no variation for all altitudes over time. Even evaluated the individuals distribution by size or growth form, the variations was mainly influenced by altitude. Individuals' density distribution was not related with slope and canopy openness variations. The individual growth of Psychotria nuda varies little over time and between altitudes. The diameter growth rate did not vary with individual's diameter class and with abiotic traits. Growth rate was positive and equals to one in both altitudes. Mortality and recruitment were similar between altitudes and did not vary in time and space. The smaller diameter classes were the most frequent ones and the reproduction probability was related with plant size. The proportion of reproductive plants was higher in Submontane Secondary-Dense-Ombrophylous Forest. Most plants remained in the original diameter class. There were some cases with regression of up to two diameter classes that may mean size reduction or ramets fragmentation. There was a little input of recruits from sexual reproduction. Almost all recruits came from vegetative propagation (91,6%). The contributions to variability in ? were different between altitudes due to distinct important transitions in each altitude. Some population traits varied more strongly with altitude than others. The differences highlight that two altitudes populations maintained stability, but from different strategies. At higher altitude, plants were larger than those on lower altitude and just the bigger ones were reproductive. At lower altitude plants start reproduction earlier than in higher altitude and had more input of recruits from vegetative propagation.
Doutorado
Ecologia
Doutor em Ecologia
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Brum, Douglas Ferreira. "O espaço de módulos de geodésicas complexas no plano hiperbólico complexo." Universidade Federal de Juiz de Fora (UFJF), 2013. https://repositorio.ufjf.br/jspui/handle/ufjf/4711.
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CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Esse trabalho visa descrever o espaço de Módulos de m-uplas geodésicas complexas distintas em H2c nos casos regular, especial e degenerado. Para tal fim faremos uso da matriz de Gram e dos invariantes (d-invariantes, δ-invariantes, invariante angular e invariantes parabólicos) que descrevem unicamente a classe de congruência de PU(2, 1) de m-uplas ordenadas de geodésicas complexas distintas nos diferentes casos supracitados.
This work aims to describe the Modules space of m-tuples distinct complex geodesics in H2c in the cases regular, special and degenerate. To this end we use the Gram matrix and the invariant (d-invariant, δ-invariants, angular invariant and parabolic invariants) that define uniquely the PU(2,1)-congruence class of ordered m-uplas of distinct complex geodesics in the different cases above.
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Muwanga, Catherine. "An assessment of Hypoxis hemerocallidea extracts, and actives as natural antibiotic, and immune modulation phytotherapies." Thesis, University of the Western Cape, 2006. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_3303_1184589097.
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In South Africa, the crude aqueous extract from Hypoxis hemerocallidea is used by AIDS patients to treat opportunistic infections, such as tuberculosis. The rapid emergence of multidrug-resistant tuberculosis, and extreme drug resistant tuberculosis, in recent years, is a major threat to human health. The treatment of TB, nosocomial bacterial infections, and fungal infections is now a clinical challenge, especially in the immuno-compromised individual. There is a dire need for novel antibiotic alternatives with phytotherapies and plant-derived compounds as potentially promising alternatives. The main objective of this study was to investigate the antimycobacterial activity of Hypoxis hemerocallidea, a South African medicinal plant, using Mycobacterium smegmatis.
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Lipus, Jan. "Návrh plánu údržbářských prací práškového granulačního kotle." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2014. http://www.nusl.cz/ntk/nusl-231365.
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The diploma thesis deals with the issues concerning implementation of the RCM (Reliability Centred Maintenance) method on large power installations (boilers and their accessories) with the use of the new AMST software tool in the conditions of a particular energy company.
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Kurudamannil, Jubal J. "Improved Robust Stability Bounds for Sampled Data Systems with Time Delayed Feedback Control." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1419012522.
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Ambrosio, Cibeli Freitas Serafim. "Governação integrada e os seus fatores críticos: Estudo de caso do Plano Estratégico para o Envelhecimento Sustentável do Município da Amadora." Master's thesis, Instituto Superior de Ciências Sociais e Políticas, 2021. http://hdl.handle.net/10400.5/21659.
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Dissertação de Mestrado em Gestão e Políticas PúblicasEste trabalho pretende analisar os fatores críticos de governação integrada propostos na Matriz GovInt, verificando a percepção dos stakeholders sobre o fator mais relevante na etapa de implementação do Plano Estratégico para o Envelhecimento Sustentável (2016-2025) – PEES. Para tanto, inicialmente foi contextualizado e caracterizado o modelo de governação integrada e seus fatores críticos. Trata-se de modelo de gestão pública que procura consolidar novas bases relacionais entre Governo e Cidadão, alterando a tradicional perspectiva unilateral (Governo > Cidadão) para uma perspectiva bilateral (Governo-Cidadão-Governo), e que tem sido defendido e aplicado especialmente para o tratamento de problemas sociais complexos. Para conduzir a investigação, foi realizada pesquisa de natureza exploratória e descritiva, com uma abordagem qualitativa pautada em técnicas de categorização, sendo a população composta por parceiros que celebraram o Pacto Local para o Envelhecimento Sustentável. A Matriz GovInt, fundamentada em quatro fatores: liderança, participação, comunicação e avaliação, foi utilizada como grelha de análise e, apesar de ser método novo, obteve boa aceitação pelos participantes. Considerando as características da população, foi realizada a triangulação de fontes de dados: questionário eletrônicos, entrevistas semiestruturadas e pesquisa documental para conferir validade ao estudo. Os resultados sugerem que os stakeholders percepcionam a presença simultânea dos quatro fatores críticos da governação integrada, conforme defende a Matriz GovInt. No entanto, divergindo do proposto nessa metodologia, que indica a liderança como a dimensão com maior peso na governação integrada, os stakeholders assinalaram a participação como o fator crítico mais influente na implementação do PEES. Além disso, a análise forneceu não apenas reflexões sobre a governação integrada e seus fatores críticos, mas também insigths sobre possíveis melhorias nas etapas subsequentes do PEES. Nesse sentido, pautando-se na percepção dos stakeholders, buscou-se também explorar possíveis porquês adjacentes à escolha do fator crítico. Dessa forma, após a categorização efectuada, foram pontuadas algumas sugestões para a etapa de implementação do PEES, dentre as quais se destacam o emprego de pequenos estímulos (“nugde”) para manter a motivação dos participantes durante o decurso do Plano Estratégico.
This research analyzes the critical factors of integrated governance proposed in the GovInt Matrix, problematizing the perception of the stakeholders about the most relevant factor in the implementation stage of the Strategic Plan for Sustainable Aging (2016-2025) - PEES. To this end, the integrated governance model and its critical factors were initially contextualized and characterized. It is a public management model that seeks to consolidate new relational bases between Government and Citizens, changing the traditional unilateral perspective (Government > Citizen) to a bilateral perspective (Government-Citizen-Government), which has been defended and applied especially for the treatment of complex social problems. To conduct the investigation, an exploratory and descriptive research was carried out, with a qualitative approach based on categorization techniques, with the population being composed of partners who celebrated the Local Pact for Sustainable Aging. The GovInt Matrix, based on four factors: leadership, participation, communication and evaluation, was used as an analysis grid and, despite being a new method, it was well accepted by the participants. Considering the characteristics of the population, data sources were triangulated: electronic questionnaires, semi-structured interviews and documentary research to give validity to the study. The results suggest that stakeholders perceive the simultaneous presence of the four critical factors of integrated governance, as advocated by the GovInt Matrix. However, diverging from what was proposed in this methodology, which indicates leadership as the most important dimension in integrated governance, stakeholders highlighted participation as the most influential critical factor in the implementation of the PEES. In addition, the analysis provided not only reflections on integrated governance and its critical factors, but also insights into possible improvements in subsequent stages of the PEES. In this sense, based on the perception of stakeholders, we also sought to explore possible whys adjacent to the choice of the critical factor. Thus, after the categorization carried out, some suggestions were made for the stage of implementing the PEES, among which the use of small stimuli (“Nudge”) to maintain the motivation of the participants during the course of implementation of the Strategic Plan.
N/A
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Costa, Wstalin Sardinha da. "Ordenamento das prioridades de tomada de decisão sob a ótica do atendimento às expectativas institucionais internas: aplicação em um instituto federal." Universidade Federal de Goiás, 2016. http://repositorio.bc.ufg.br/tede/handle/tede/6387.
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The constant improvement of business services depends on its ability to analyze perceived quality’s most influential aspects for customer as well as for other business process’ parties. It is no different in higher education institutions, since its importance to society – high quality education can improve a country’s labor relations, technological capacity, production, and employment levels. This study intends to propose a service’s quality management analysis tool for academic internal stakeholders, in a higher education basis, of a Federal Education Institute. The chosen academic internal stakeholders were: Undergraduate students, Administrative staff, Teachers and Management Group. This paper analyzed the main quality assessment models - including Cameron’s (1978), SINAES, and Parasuraman Zeithaml, Berry’s (1985) - where the research’s variables and quality indicators were extracted from. Then, basing on Zeithaml, Parasuraman and Berry’s (1990) Quality’s Gap and Slack’s (1994) Opportunity Matrix models, the stakeholder’s attributes of importance and performance were compared. Thus, the results from the research presented Adequate, Improvement, and Immediate Action attributes in the Opportunity Matrix. Lastly, the research focused on the Immediate Action attributes – Accessibility for people with disabilities; Access to computer equipment, internet and Wi Fi; Life quality; Staff training; Cooperative environment; Students’ Moral; Problem solving ability; Availability of an efficient virtual system; Intermediation with the labor market; Fluidity of interdepartmental communication; Punctuality of Students; Use of talent and expertise; communication flow with the Management Group; Management plan compliance; Flexibility in assembling the curriculum – to propose an action plan based on BSC methodology that shows intervention initiatives for the Institute researched.
A melhoria contínua dos serviços prestados por uma organização depende da sua capacidade de analisar os aspectos com maior influência sobre a qualidade percebida, não só por seus clientes, mas pelas partes envolvidas no processo produtivo. Isso não é diferente em instituições de ensino superior, tamanha a importância dessas organizações para a sociedade – uma educação de qualidade é capaz de melhorar as relações de trabalho, a capacidade tecnológica, produtiva e de emprego de um país. O presente estudo tem como objetivo propor a implantação de um instrumento de análise da gestão da qualidade dos serviços prestados entre os stakeholders da comunidade acadêmica interna, em nível de graduação, de um Instituto Federal de Educação. Foram pesquisados os stakeholders Alunos de graduação, Técnicos Administrativos, Professores e Grupo Gestor. Fez-se uma análise dos principais modelos de avaliação de qualidade – entre eles o de Cameron (1978), o SINAES, e o de Parasuraman, Zeithaml e Berry (1985) – de onde se extraiu as variáveis de análise e os indicadores de qualidade analisados na pesquisa. Baseou-se, então, a análise de qualidade no modelo de lacunas de Zeithaml, Parasuraman e Berry (1990) e no modelo de matrizes de oportunidades de Slack (1994), comparando o grau de importância e o desempenho conferido a cada atributo, para cada stakeholder pesquisado. Com isso, chegou-se a uma matriz de importância que demonstrou atributos Adequados, de Melhoramento, e de Ação Imediata. Por fim, focou-se nos atributos de Ação Imediata – Acessibilidade a pessoas com deficiência; Acesso a equipamentos de informática, internet e Wi Fi; Qualidade de vida; Treinamento e capacitação; Ambiente cooperativo; Moral dos Alunos; Capacidade de solução de problemas; Disponibilidade e eficiência de sistema virtual; Intermediação com mercado de trabalho; Fluidez de comunicação interdepartamental; Pontualidade dos Alunos; Uso de talento e perícia; Fluidez de comunicação com o Grupo Gestor; Cumprimento do plano de gestão; Flexibilidade na montagem da grade curricular – para propor um plano de ação baseado na metodologia BSC com iniciativas de intervenção para o instituto pesquisado.
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Luz, J?lia Lins. "Influ?ncia de Planta??es de Banana na assembleia de morcegos (Chiroptera) e na dieta e dispers?o de sementes." Universidade Federal Rural do Rio de Janeiro, 2012. https://tede.ufrrj.br/jspui/handle/jspui/1495.
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Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq
Modification of the landscape for food production and other facilities for human consumption is the most common and severe threat to global biodiversity. The aims of this study were to analyze how banana plantations affect bats assemblages, to check whether the planting of bananas causes a decrease in the dispersion of seeds, to verify if the abundance of nutritional resources have some influence on bat abundance and richness in areas of banana plantation, to verify if bats move between plantations and forest fragments or if they show site fidelity to the areas. A total of 12 banana plantations in Rio de Janeiro State were sampled. Each month, from November 2008 to October 2010, two capture nights were conducted, being one night in a banana plantation area and another in an adjacent forest fragment. Bats were sampled with mist nets, totaling 142,560 m2.h of sampling effort. Bats were identified, marked, remained in cloth bags to obtain fecal samples, and released after it. The seeds obtained in fecal samples were counted and classified. The availability of food resources in banana plantation areas was estimated in five 100 m2 plots, where the number of banana trees, flowers and fruits was counted. A total of 2,369 captures and recaptures of bats of 27 species were recorded. In banana plantations 22 species were recorded (four of which were exclusive to such habitat), and in fragments 23 (with five exclusive). The capture frequency was higher in plantations than in fragments. Richness, diversity and evenness were similar between habitats. Artibeus lituratus, Carollia perspicillata, Glossophaga soricina and Phyllostomus hastatus were more abundant in the banana plantations. The abundance of frugivores, nectarivores and omnivores was higher in plantation areas while insectivores were more abundant in forest areas. The estimated richness for each environment suggests that forest fragments are richer than the banana plantations. A non-metric multidimensional scaling (NMDS) indicated separation between banana plantations and forest fragments based on species abundance, using Braycurtis similarity. We analyzed 1,127 fecal samples from 20 species that had 33 morphotypes of seeds of 12 plant families. There was presence of banana pulp in fecal samples from 10 species. The richness and abundance of seeds dispersed in the two environments did not differ, however the abundance of seeds per fecal sample was lower in banana plantations. The abundance of Sturnira lilium was correlated negatively with the abundance of banana and fruits. The abundance of G. soricina, A. lituratus, nectarivorous and frugivorous bats showed positive relationship with the abundance of food resource. We obtained 102 recaptures of 99 individuals of 10 species. The most recaptured species were C. perspicillata, A. lituratus and P. hastatus. Twenty-eight individuals moved between environments, 68 remained in the same environment of the capture. In all analyzed cases, the proportion of individuals recaptured in the same location of capture was higher than the recapture proportion in different environments. These results reinforce the potential of bats as regenerators in habitats modified by humans, since banana plantations maintain a diverse assemblage. The ability of bats to disperse pioneer plants species and the use of different environments assure that nature services of forest regeneration are continued
A modifica??o da paisagem para a produ??o de alimentos e outras comodidades para consumo humano representa a mais severa e comum amea?a ? biodiversidade global. Os objetivos deste trabalho foram analisar como o plantio de bananas afeta a comunidade de morcegos, analisar se o plantio de bananas diminui a dispers?o de sementes, verificar se a abund?ncia de recurso alimentar influencia a abund?ncia e a riqueza de morcegos em ?reas de planta??o de banana, verificar se os morcegos se deslocam entre ?reas de planta??es e fragmentos florestais e se mant?m fidelidade ? ?rea de captura. Foram selecionadas 12 ?reas de planta??o do estado do Rio de Janeiro. Mensalmente, entre novembro de 2008 e outubro de 2010, foram realizadas duas noites de coleta, sendo uma em ?rea de planta??o de banana e outra em um fragmento florestal adjacente. As coletas foram realizadas com redes de neblina, totalizando 142.560 m2.h de esfor?o amostral. Os morcegos foram identificados, permaneceram em sacos de pano para obten??o de amostras fecais, e soltos ap?s receberem marca??o. No laborat?rio, as sementes foram classificadas em morfotipos e contadas. A disponibilidade de recurso alimentar nas ?reas de planta??o de banana foi estimada atrav?s de cinco plots de 100 m2 , nos quais era contabilizado o n?mero de bananeiras, infrutesc?ncias e infloresc?ncias de banana. Um total de 2.369 capturas e recapturas de 27 esp?cies foi registrado. Nos bananais, foram capturadas 22 esp?cies, sendo quatro exclusivas; nos fragmentos, 23, sendo cinco exclusivas. A frequ?ncia de captura nos bananais foi maior do que nos fragmentos. A riqueza, a diversidade e a equitabilidade mostraram-se similares. As esp?cies significativamente mais abundantes nos bananais foram Artibeus lituratus, Carollia perspicillata, Glossophaga soricina e Phyllostomus hastatus. A abund?ncia de frug?voros, nectar?voros e on?voros foi maior em ?reas de plantio, enquanto a de inset?voros foi maior em ?reas de floresta. A riqueza estimada para cada ambiente sugere que os fragmentos florestais s?o mais ricos do que as ?reas de plantio. Uma an?lise de escalonamento multidimensional (NMDS) indicou separa??o entre os bananais e os fragmentos florestais baseado no n?mero de capturas por esp?cie, atrav?s da dist?ncia Bray-curtis. Foram analisadas 1.127 amostras fecais de 20 esp?cies, que apresentaram sementes de 33 morfotipos de 12 fam?lias de plantas. Foi poss?vel verificar a presen?a de polpa de banana em amostras fecais de 10 esp?cies. A riqueza e abund?ncia de sementes dispersadas nos dois ambientes n?o diferiram. No entanto, a abund?ncia de sementes por amostra fecal foi menor em ?reas de planta??o de banana. A abund?ncia de Sturnira lilium foi inversamente proporcional ? abund?ncia de bananeiras e infrutesc?ncias de banana. A abund?ncia de G. soricina, A. lituratus, morcegos nectar?voros e frug?voros foi diretamente proporcional ? abund?ncia de recurso alimentar. Foram obtidas 102 recapturas de 99 morcegos de 10 esp?cies. As esp?cies mais recapturadas foram C. perspicillata, A. lituratus e P. hastatus. Vinte e oito indiv?duos transitaram entre os ambientes, 68 permaneceram no mesmo ambiente da captura e tr?s foram recapturados duas vezes, uma em cada ambiente. Em todos os casos analisados, a propor??o de indiv?duos recapturados no mesmo local da captura foi maior do que a propor??o de indiv?duos recapturados em ambientes diferentes. Em ?reas de planta??o de banana, a ordem Chiroptera foi capaz de manter uma comunidade diversa, a capacidade de dispers?o de esp?cies pioneiras de plantas e a capacidade de deslocamento. Esses resultados refor?am o potencial dos morcegos como regeneradores de habitats modificados pelo homem.
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Wang, Ming Chang. "The application of elementary stiffness matrix decomposition method in stochastic plane stress problems." Thesis, University of Macau, 2009. http://umaclib3.umac.mo/record=b2119803.
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Leclerc, Céline. "Étude et conception de matrices d'alimentation multifaisceaux pour réseaux à rayonnement direct ou dans le plan focal d'un réflecteur." Phd thesis, Toulouse, INPT, 2013. http://oatao.univ-toulouse.fr/10907/1/leclerc.pdf.
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Dans le cadre de cette thèse, on s'intéresse dans un premier temps à des matrices d'alimentation de type passif connues qui permettent de produire des faisceaux orthogonaux, et notamment à la matrice de Butler. On s'aperçoit qu'il n'existe qu'une méthode itérative permettant de déterminer les paramètres S d'une matrice de Butler symétrique. C'est pourquoi, on cherche à déterminer de manière analytique la matrice [S] d'une matrice de Butler symétrique à N = 2^n entrées et N sorties. On établit des formules de récurrence issues de l'étude de ce type de matrices pour plusieurs tailles. Des formules analytiques en sont déduites qui sont valables quelle que soit la valeur de N. On poursuit l'étude des matrices d'alimentation de réseau d'antennes en s'intéressant à la source focale d'une antenne multifaisceaux devant réflecteur basée sur une géométrie originale constituée de coupleurs directionnels tridimensionnels entrelacés. Cette structure est simulée. Ses nombreux paramètres sont optimisés afin d'aboutir à une solution répondant au cahier des charges. Une maquette est réalisée et testée. Les résultats obtenus sont prometteurs. Au final, cette source possède l'avantage d'avoir un fort niveau de réutilisation de ses éléments rayonnants et donc de limiter l'encombrement du système global qui est souvent un point critique, dans les satellites notamment. Pour finir, on s'intéresse à une structure dont le but est d'avoir une station au sol capable de suivre une cible sans dépointage. La poursuite en azimut est assurée par une partie mécanique, de type joint tournant ; celle en élévation par un module électronique. Le système se doit d'être compact, fiable, et de limiter les pertes ainsi que les coûts. Des compromis sont donc à effectuer. La partie rayonnante est réalisée en guide, la partie alimentation en technologie planaire. Une solution est proposée afin de faire la transition entre ces deux technologies : le circuit de répartition est directement relié à l'excitation des éléments rayonnants en mettant ces deux parties sur une même feuille de substrat. Ainsi, moins de câbles et de connecteurs sont nécessaires, ce qui diminue l'encombrement et les coûts.
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Thompson, Ronald H. "Plane frame element addition to the MESS finite element program." Thesis, Virginia Polytechnic Institute and State University, 1985. http://hdl.handle.net/10919/80052.
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A plane frame element based on linear, elastic theory is developed and implemented into the MESS finite element program. Post-processed results include nodal displacements, end reactions, maximum tensile and average shear stress, and a deformed geometry plot. The element is tested for accuracy relative to simple beam theory and by comparison with results generated using another finite element program. In both cases agreement to within 6 significant figures was achieved.
Because the intended use is educational, a survey of its benefit as a design aid in undergraduate instruction is included. These benefits are based on test cases from senior design class projects. Results generated using analysis techniques presently available are contrasted with those using the plane frame element. Students' work that was examined contained mistakes resulting from large amounts of hand calculations. Conversely, results generated using the finite element method proved to be easily obtained and to have a higher degree of accuracy. A recommendation for further improvements in program capability is provided at the end of the study.Master of Science
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Seghini, Maria Carolina. "Mechanical Analysis and Fibre/Matrix Interface Optimization for Next Generation of Basalt-Plant Fibre Hybrid Composites." Electronic Thesis or Diss., Chasseneuil-du-Poitou, Ecole nationale supérieure de mécanique et d'aérotechnique, 2020. http://www.theses.fr/2020ESMA0003.
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La prise de conscience mondiale des enjeux environnementaux a conduit à l’émergence de composites«verts», dans lesquels les fibres naturelles sont amenées à remplacer les fibres synthétiques. Ces nouveaux matériaux offrent des alternatives écologiques aux composites synthétiques traditionnels mais sont difficilement utilisables pour des applications semi-structurales ou structurales. Une solution possible à ce problème est le développement des composites hybrides, en combinant ensemble fibres naturelles et synthétiques. Dans ce cadre, l'objectif de cette étude était de développer des composites hybrides à base de fibres de basalte et de lin. Les composites hybrides ont été élaborés par moulage par infusion sous vide avec une matrice époxy. À des fins de comparaison,des composites 100% à fibres de lin et100%à fibres de basalte ont également été produits. Une caractérisation mécanique quasi-statique et dynamique amontré que l'hybridation permet d’obtenir un composite avec des propriétés mécaniques intermédiaires comparées à celles des composites à fibres de lin ou de basalte. Cependant, l’analyse approfondie des dommages a montré la nécessité d'optimiser la qualité d'adhésion de l'interface fibre/matrice afin d'accroître les performances mécaniques des composites hybrides obtenus. Pour cette raison, différents traitements de modification de surface ont été développés et étudiés pour les fibres de lin et de basalte. Un traitement physique par plasma (Plasma Enhanced Chemical Vapor Deposition) a été appliqué aux fibres de lin et de basalte. Les fibres de lin ont également été soumises à deux traitements chimiques utilisant des espèces enzymatiques et du CO2supercritique. Les effets des traitements sur la stabilité thermique, la morphologie et les propriétés mécaniques des fibres de lin et de basalte ont été étudiés. L’adhérence fibre/matrice a été analysée en réalisant des tests de fragmentation sur des composites monofilamentaires. La qualité de l'adhésion entre les fibres et les matrices époxy et vinylester a été évaluée en termes de longueur critique de fragment, de longueur de décohésion interfaciale et de résistance au cisaillement interfacial. La micto-tomographie haute résolution a été utilisée pour analyser les mécanismes d'endommagement lors des tests de fragmentation. Pour les deux types de fibres, les meilleurs résultat sont été obtenus grâce au traitement par plasma. Ce traitement a consisté à déposer un revêtement homogène de tétravinylsilane à la surface des fibres de basalte et de lin, ce qui a permis une augmentation significative de l’adhérence fibre/matrice, ouvrant ainsi la voie à la prochaine génération de composites hybrides plus respectueux de l’environnement et utilisables pour des applications semi-structuralesGlobal awareness of environmental issues has resulted in the emergence of “green” composites, in which natural fibres are used to replace synthetic ones. However, in semi-or structural applications, it can be inconvenient to use composites based on natural fibres. A possible solution to this problem is the development of hybrid composite materials, combining together plies of natural and synthetic fibres. In this framework, the aim of this research project was to develop basalt-flax fibre hybrid composites with a view to obtaining more environmentally friendly composites for semi-structural applications. Hybrid composites were produced through vacuum infusion molding with epoxy matrix.For comparison purposes, 100% flax fibre composites and 100% basalt fibre composites were also manufactured. A quasi-static and dynamic mechanical characterization showed that the hybridization allows the production of a composite with intermediate mechanical performances compared to those possessed by flax and basalt composites. However, the damage analysis has revealed the need to optimize the fibre/matrix interface adhesion quality, in order to increase the mechanical properties of the resulting hybrid composites. For this reason, different surface modification treatments have been specifically designed and investigated for flax and basalt fibres. Flax and basalt fibres were treated by the physical process of Plasma Enhanced Chemical Vapor Deposition. Flax fibres were also subjected to two chemical treatments using enzymatic species and supercritical CO2. The effects of the surface modification treatments on the thermal stability, morphology and mechanical properties of flax and basalt fibres have been investigated. The degree and extent of fibre/matrix adhesion were analyzed by micromechanical fragmentation tests on monofilament composites. The adhesion quality between fibres and both epoxy and vinylester matrices has been assessed in terms of critical fragment length, debonding length and interfacial shear strength. High-resolution μ-CT has been used to support the analysis of the damage mechanisms during fragmentation tests. For both flax and basalt fibres, the best results were obtained after the plasma polymer deposition process. This process was able to produce a homogeneous tetravinylsilane coating on the surface of basalt and flax fibres, which resulted in a significant increase in the fibre/matrix adhesion, thus paving the way for the next generation of more environmentally friendly hybrid composites for semi-structural applications
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Li, Han. "Transfer Matrix Approach to Propagation of Angular Plane Wave Spectra Through Metamaterial Multilayer Structures." University of Dayton / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=dayton1324508726.
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Mbandezelo, Mongikazi. "Bioactive compounds in a Manayi traditional medicinal product from East London." Thesis, Cape Peninsula University of Technology, 2016. http://hdl.handle.net/20.500.11838/2450.
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Thesis (MTech (Chemistry))--Cape Peninsula University of Technology, 2016.In Africa, herbal medicines are often used as primary treatments for a variety of ailments and diseases including HIV/AIDS and for HIV-related problems. In general, traditional medicines are not well researched scientifically in controlled studies, and are poorly regulated. Since the pharmacological effectiveness of natural products is affected by several native and foreign factors, studies on the variations of chemical composition and biological activity of these medicines are necessary. The processes of investigating plants to identify chemical substances are of great interest to natural product researchers because there is a need to discover new drugs for treating old and new diseases. These facts underscore an urgent need to develop new anti HIV and AIDS drugs with fewer or no side effects. Research into drug discovery and development using natural products is increasingly becoming better established. Marine organisms as a source of natural products delivered numerous novel compounds with multiple pharmacological properties. Natural products give endless opportunities for discovering novel compounds that can be used as drugs or backbones of drug leads. Manayi is a natural product that has been used to treat and manage people with HIV, but no scientific studies have been done to prove its efficacy on the HIV under controlled conditions. For the purposes of this study, cooked and uncooked Manayi product was evaluated for its efficacy on HIV in vitro. Manayi samples were collected in East London, Eastern Cape and sequentially extracted with hexane, chloroform, dichloromethane, butanol, methanol, and water as a series of increasingly polar solvents for its bioactive chemical constituents.
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Duval, Johanna. "Eco-valorisation de la plante Kniphofia uvaria : de la plante à la galénique." Thesis, Orléans, 2016. http://www.theses.fr/2016ORLE2045/document.
Full textAbstract:
À l’heure où l’intégration des enjeux environnementaux dans le développement de procédés éco-efficients joue un rôle essentiel dans le moteur de l’innovation responsable, la chimie verte est devenue l’un des sujets de préoccupation majeure. Ainsi, le développement de nouveaux procédés éco-respectueux pour la production d’ingrédients naturels issus de matières premières végétales renouvelables est devenu une démarche incontournable dans le modèle de recherche. L’objectif de cette thèse a consisté au développement d’une stratégie d’éco-valorisation innovante employant les fluides sub/supercritiques pour l’extraction, la caractérisation, la production et l’imprégnation sur support cosmétique de produits naturels d’origine végétale. Pour cela, nous avons utilisé comme modèle végétal : les graines oléagineuses de la plante Kniphofia uvaria, sélectionnée pour des applications cosmétiques grâce à ses propriétés bioactives antioxydantes et anti-âge. Dans un premier temps, le développement de méthodes complémentaires en SFC ainsi que le développement du couplage SFC-MS a été réalisé à l’aide de la source APCI afin d’identifier les molécules responsables des activités bioactives des graines de Kniphofia uvaria. Ainsi, le développement d’un système hybride (U)HPLC/SFC-HRMS a été réalisé afin de mettre en place ce couplage. Des optimisations en termes de proportion et nature de solvant make-up ainsi qu’un travail au niveau des paramètres SFC et MS ont été faits afin de d’améliorer la sensibilité et la spécificité des analyses lipidiques. Dans un second temps, nous nous sommes attachés au développement d’une stratégie d’enrichissement en composés bioactifs à l’aide des méthodes : SFE et CPC. Ainsi, en SFE, des optimisations en termes de température, pression, nature/proportions de co-solvant dans le fluide ont été réalisées alors qu’en CPC, des optimisations au niveau de l’injection ont été faites. Des conditions optimales pour le fractionnement sélectif des anthraquinones et des triglycérides ont été déterminées en SFE et CPC. Dans un dernier temps, ce travail a consisté à développer un couplage en-ligne pour extraire et imprégner sélectivement sur silice cosmétique : les anthraquinones. Le développement et l’optimisation de ce procédé en-ligne ont été réalisés à l’échelle du laboratoire et ont démontré la faisabilité de ce couplage ainsi qu’un intérêt certain pour l’obtention de produits naturels sous une première forme galénique, destinée à une future incorporation dans la formulation de cosmétiquesNowadays, green chemistry is a great challenge. It seeks innovation in the development of eco-efficient processes. The production of natural products from renewable materials by these new environmentally friendly processes is more and more used. The aim of this Ph.D thesis is to develop an eco-valuation strategy to extract, characterize, produce and impregnate natural products onto a cosmetic support using sub/supercritical fluids. Consequently, we used oleaginous plant seeds from Kniphofia uvaria as a plant model, which was selected for its interesting cosmetic properties such as antioxidant or anti-ageing. Firstly, the SFC-MS hyphenation with the APCI as an ionization source was developed to screen bioactive molecules; responsible of cosmetic properties. This coupling was performed by the hybrid combination of (U)HPLC/SFC-HRMS. Various optimizations in terms of the solvent make-up (nature and proportion), modulation with SFC and MS parameters were carried out in order to improve sensitivity and selectivity of lipid analysis. Secondly, an enrichment strategy to concentrate bioactive compounds in the final extract was developed by SFE and CPC. Thus, in SFE, experimental parameters (temperature, pressure, nature/proportion of the modifier in the CO2 fluid) were optimized while in CPC, the injection optimization was realized. Methods for the selective fractionation of anthraquinones and triglycerides were obtained in CPC and SFE. Finally, an on-line sub/supercritical extraction-impregnation process was developed to extract and for simultaneously impregnating anthraquinones onto a cosmetic silica. Development and optimization of this process was realized on a laboratory scale. Consequently, this study demonstrated the feasibility of this concept and it presents a great interest to provide natural products as a galenic form, which could be used in the cosmetic formulation