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Relevant bibliographies by topics / Plant pathogenic fungus

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Academic literature on the topic 'Plant pathogenic fungus'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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Journal articles on the topic "Plant pathogenic fungus"

1

Fonseca, Sandra, Dhanya Radhakrishnan, Kalika Prasad, and Andrea Chini. "Fungal Production and Manipulation of Plant Hormones." Current Medicinal Chemistry 25, no. 2 (2018): 253–67. http://dx.doi.org/10.2174/0929867324666170314150827.

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Living organisms are part of a highly interconnected web of interactions, characterised by species nurturing, competing, parasitizing and preying on one another. Plants have evolved cooperative as well as defensive strategies to interact with neighbour organisms. Among these, the plant-fungus associations are very diverse, ranging from pathogenic to mutualistic. Our current knowledge of plant-fungus interactions suggests a sophisticated coevolution to ensure dynamic plant responses to evolving fungal mutualistic/pathogenic strategies. The plant-fungus communication relies on a rich chemical language. To manipulate the plant defence mechanisms, fungi produce and secrete several classes of biomolecules, whose modeof- action is largely unknown. Upon perception of the fungi, plants produce phytohormones and a battery of secondary metabolites that serve as defence mechanism against invaders or to promote mutualistic associations. These mutualistic chemical signals can be co-opted by pathogenic fungi for their own benefit. Among the plant molecules regulating plant-fungus interaction, phytohormones play a critical role since they modulate various aspects of plant development, defences and stress responses. Intriguingly, fungi can also produce phytohormones, although the actual role of fungalproduced phytohormones in plant-fungus interactions is poorly understood. Here, we discuss the recent advances in fungal production of phytohormone, their putative role as endogenous fungal signals and how fungi manipulate plant hormone balance to their benefits.

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2

LATG, JEAN-PAUL. "Aspergillus fumigatus, a saprotrophic pathogenic fungus." Mycologist 17, no. 2 (2003): 56–61. http://dx.doi.org/10.1017/s0269915x0300209x.

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3

Chen, Fangfang, Ruijing Ma, and Xiao-Lin Chen. "Advances of Metabolomics in Fungal Pathogen–Plant Interactions." Metabolites 9, no. 8 (2019): 169. http://dx.doi.org/10.3390/metabo9080169.

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Plant disease caused by fungus is one of the major threats to global food security, and understanding fungus–plant interactions is important for plant disease control. Research devoted to revealing the mechanisms of fungal pathogen–plant interactions has been conducted using genomics, transcriptomics, proteomics, and metabolomics. Metabolomics research based on mass spectrometric techniques is an important part of systems biology. In the past decade, the emerging field of metabolomics in plant pathogenic fungi has received wide attention. It not only provides a qualitative and quantitative approach for determining the pathogenesis of pathogenic fungi but also helps to elucidate the defense mechanisms of their host plants. This review focuses on the methods and progress of metabolomics research in fungal pathogen–plant interactions. In addition, the prospects and challenges of metabolomics research in plant pathogenic fungi and their hosts are addressed.

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Abdoulaye, Assane Hamidou, Mohamed Frahat Foda, and Ioly Kotta-Loizou. "Viruses Infecting the Plant Pathogenic Fungus Rhizoctonia solani." Viruses 11, no. 12 (2019): 1113. http://dx.doi.org/10.3390/v11121113.

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The cosmopolitan fungus Rhizoctonia solani has a wide host range and is the causal agent of numerous crop diseases, leading to significant economic losses. To date, no cultivars showing complete resistance to R. solani have been identified and it is imperative to develop a strategy to control the spread of the disease. Fungal viruses, or mycoviruses, are widespread in all major groups of fungi and next-generation sequencing (NGS) is currently the most efficient approach for their identification. An increasing number of novel mycoviruses are being reported, including double-stranded (ds) RNA, circular single-stranded (ss) DNA, negative sense (−)ssRNA, and positive sense (+)ssRNA viruses. The majority of mycovirus infections are cryptic with no obvious symptoms on the hosts; however, some mycoviruses may alter fungal host pathogenicity resulting in hypervirulence or hypovirulence and are therefore potential biological control agents that could be used to combat fungal diseases. R. solani harbors a range of dsRNA and ssRNA viruses, either belonging to established families, such as Endornaviridae, Tymoviridae, Partitiviridae, and Narnaviridae, or unclassified, and some of them have been associated with hypervirulence or hypovirulence. Here we discuss in depth the molecular features of known viruses infecting R. solani and their potential as biological control agents.

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Rohe, M., J. Searle, A. C. Newton, and W. Knogge. "Transformation of the plant pathogenic fungus, Rhynchosporium secalis." Current Genetics 29, no. 6 (1996): 587–90. http://dx.doi.org/10.1007/s002940050089.

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Fuchs, U., and G. Steinberg. "Endocytosis in the plant-pathogenic fungus Ustilago maydis." Protoplasma 226, no. 1-2 (2005): 75–80. http://dx.doi.org/10.1007/s00709-005-0109-3.

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Rohe, M., J. Searle, A. C. Newton, and W. Knogge. "Transformation of the plant pathogenic fungus,Rhynchosporium secalis." Current Genetics 29, no. 6 (1996): 587–90. http://dx.doi.org/10.1007/bf02426964.

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8

Farooq, Afgan, Iqbal Choudhary, Atta-ur Rahman, Satoshi Tahara, K. Hüsnü Can Başer, and Fatih Demirci. "Detoxification of Terpinolene by Plant Pathogenic Fungus Botrytis cinerea." Zeitschrift für Naturforschung C 57, no. 9-10 (2002): 863–66. http://dx.doi.org/10.1515/znc-2002-9-1018.

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Detoxification of an antifungal monoterpene terpinolene (1) by the plant pathogenic fungus Botrytis cinerea afforded hydroxlyated metabolites 2,3-dihydro-3β,6β-dihydroxy-terpinolene (2) (39%) and 2,3-dihydro-1α,3α-dihydroxy-terpinolene (3) (20%), respectively. Terpinolene showed good levels of antifungal activity while both the metabolites were inactive against another plant pathogenic fungus Cladosporium herbarum.

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9

Ekici, Tuğba. "Light and Electron Microscope Studies of Species of Plant Pathogenic Basidiomycota Isolated from Plants in Kıbrıs Village Valley (Ankara, Turkey)." Journal of Fungus 5, no. 1 (2014): 7. http://dx.doi.org/10.15318/fungus.201456196.

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H.Ighachane, H. Ighachane, H. El ayadi H.El ayadi, My H. Sedra My.H.Sedra, and H. B. Lazrek H.B.Lazrek. "Biological Evaluation of 1, 4-Disubstituted 1,2,3-Triazole Derivatives as Plant Pathogenic Fungus Inhibitors." Indian Journal of Applied Research 4, no. 8 (2011): 685–88. http://dx.doi.org/10.15373/2249555x/august2014/201.

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Dissertations / Theses on the topic "Plant pathogenic fungus"

1

Carlile, Amanda Jane. "Cell wall degrading enzymes of the plant pathogenic fungus Stagonospora nodorum." Thesis, University of Bath, 1999. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299845.

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Sjöberg, Johanna. "Arbuscular mycorrhizal fungi : occurrence in Sweden and interaction with a plant pathogenic fungus in barley /." Uppsala : Dept. of Crop Production Ecology, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/200533.pdf.

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3

Way, John Alexander. "Investigating a microbial fungicide to enhance biological control of plant disease." Thesis, University of Surrey, 2000. http://epubs.surrey.ac.uk/843864/.

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The antibiotic, 2,4-diacetylphloroglucinol (Phl), is produced by a range of naturally isolated fluorescent pseudomonads, found in disease suppressive soils. The natural isolate, P. fluorescens F113, protects pea plants from the pathogenic fungus, Pythium ultimum, by reducing the number of pathogenic lesions on the plant's roots. This beneficial effect was however, outweighed by the F113 causing an overall reduction in the emergence of the pea plants in the infected soil. The gene locus responsible for the Phl production was shown to be functionally conserved between the P. fluorescens F113 and another Phl producing organism, P. fluorescens Q2-87. Following identification of this functional sequence homology, the genes were isolated from F113, by optimised, long PCR. The 6.7-kb gene cluster was inserted into the chromosome of a non-pathogenic P. fluorescens, SBW25, which can effect biological control against the plant pathogen, Pythium ultimum through competitive exclusion of the fungus, by means of its strong colonising competence. The insertion was a targeted, homologous recombination designed to insert the Phl coding genes, from the F113, into a non-essential, lacZY coding region of the SBW25 chromosome. The transformed strains of SBW25 assumed two different morphological appearances. The morphological changes were noted at a ratio of 1:1 of normal morphology and altered morphology. Transformation of SBW25 with the Phl locus without this repressor element led to transformants with only normal morphology. All transformants were able to suppress P. ultimum through antibiotic production following the Phl transformation. However, the fitness of the transformants was reduced in flask culture, at 30°C, against the un-transformed SBW25. The organisms transformed with the entire Phl locus were seen to clump together in the culture media. The strain transformed with the Phl locus lacking the repressor element behaved normally. When inoculated on pea seedlings, the strain containing no repressor element behaved similarly to the F113, causing lower pea seed emergence. A transformant containing the entire Phl genetic locus had not lost its environmental competence on the pea roots, maintaining a high population, but was unable to maintain a high population in the surrounding soil.

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Lacey, Georgina Anne. "Analysis of translation elongation factor genes from the plant pathogenic fungus Magnaporthe grisea." Thesis, University of Kent, 2003. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.632130.

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Reyes, Gaige Andres. "Molecular interactions between the pathogenic fungus Macrophomina phaseolina and its plant host Medicago truncatula." Thesis, Wichita State University, 2010. http://hdl.handle.net/10057/3701.

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Macrophomina phaseolina is a necrotrophic soil-borne fungal pathogen that causes a disease commonly known as charcoal rot. This fungus has the potential to infect over 500 different plant species worldwide including many important crops such as soybean, corn and sorghum. The fungal infection dramatically decreases the yield of a crop due to loss in biomass, low seed quality and plant death. Currently, there is not an effective method for controlling the disease, because knowledge about the pathogen, the development of the disease and how it interacts with the plant host is limited. Therefore, a study was proposed to investigate the interactions between M. phaseolina and the model legume Medicago truncatula, following a molecular genetics approach where the host genes involved in the disease development will be identified. We conducted a genetic screen in a mutant population of M. truncatula to look for strains that have altered susceptibility to M. phaseolina. The initial screen of 259 Tnt1 lines identified seven lines that showed altered susceptibility to M. phaseolina. However, the second screen did not corroborate the results that were first obtained, and none of the seven Tnt1 lines showed altered susceptibility to the fungus. In addition, we screened 174 M. truncatula wild type ecotypes. As was expected, these ecotypes did not show altered susceptibility to M. phaseolina. Finally, we examined the effects of phytohormones jasmonic acid (JA) and ethylene (ET) in M. truncatula when infected with the M. phaseolina. Our results indicated that JA and ET slightly increased the resistance of the plants to this fungal pathogen. This makes us think that the modification of JA or ET signaling pathways may improve plant resistance to M. phaseolina. This study provides a basis for a further investigation of molecular interactions between M. phaseolina and its plant hosts.<br>Thesis (M.S.)--Wichita State University, College of Liberal Arts and Sciences, Dept. of Biological Sciences.

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Meyn, Malcolm Anthony 1967. "A genetic, biochemical, and population analysis of MGL, a non-LTR retroelement from the plant pathogenic fungus Magnaporthe grisea." Diss., The University of Arizona, 1997. http://hdl.handle.net/10150/288755.

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This dissertation describes the characterization of a novel transposable element isolated from the plant pathogenic fungus Magnaporthe grisea. The sequence of MGR583, a previously reported repeated DNA fragment, was completed and shown to have features characteristic of non-LTR retroelements (LINEs). These include an element length of 5.9 kb, the lack of flanking long terminal repeats, the presence of short (6-13 bp) direct repeats flanking many element copies, and two principal open reading frames (ORFs). The first ORF is 570 amino acids in length and contains homology to the gag ORFs found in many retroelements. The second ORF is 1,295 amino acids in length and has strong homology to reverse transcriptases (RT) ORFs found in non-LTR retroelements (LINEs). In accordance with these results, the name of the repeat was changed to MGL for Magnaporthe grisea LINE. Analysis of the 3' terminus of MGL showed 90% homology to the 3' terminus of Mg-SINE, suggesting an evolutionary relationship between these two elements. A survey of the distribution of MGL in populations of M. grisea showed the element to be present in all isolates tested. Copy number was not uniform between isolates, with approximately fifty copies present in rice isolates and between less than 10 and up to 50 copies in the 17 non-rice isolates tested. A PCR-based assay was designed and used to screen M. grisea isolates for polymorphic MGL insertion loci. Thirteen polymorphic MGL insertions were scored and used to construct a phylogenetic tree that included 11 non-rice isolates and 20 rice isolates. The results strongly suggested that development of virulence on rice was a single event correlated with the acquisition of virulence on several other grass species. In addition, the observation that rearrangements occurred at one of the insertion loci in some rice isolate strains support the proposal that there is considerable plasticity in the genomes of these isolates. Finally, a yeast transposon ( Tyl) system was used to express and test the second ORF for RT activity. No activity was detected for any of the MGL RT constructs tested.

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Inyang, Enenwan N. "Effect of the host plant on the susceptibility of crucifer pests to the insect pathogenic fungus, Metarhizium anisopliae." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245611.

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8

Nur, Sabrina Ahmad Azmi. "Studies on an effector NLP1 expressed during the late phase of plant infection by Colletotrichum orbiculare." Kyoto University, 2018. http://hdl.handle.net/2433/233850.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第21311号<br>農博第2296号<br>新制||農||1064(附属図書館)<br>学位論文||H30||N5145(農学部図書室)<br>京都大学大学院農学研究科応用生物科学専攻<br>(主査)教授髙野義孝, 教授田中千尋, 教授寺内良平<br>学位規則第4条第1項該当

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Neto, AntÃnio Viana Lopes. "Atividade fungistÃtica de uma quitinase recombinante do feijÃo de corda [Vigna unguiculata (L.) (Walp.)] contra Lasiodiplodia theobromae Pat. (Griff. e Maubl.), agente causal da resinose do cajueiro (Anacardium occidentale L.)." Universidade Federal do CearÃ, 2014. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=13310.

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CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior<br>O objetivo deste trabalho foi avaliar a atividade biolÃgica de uma quitinase recombinante (rVuChi) de feijÃo-caupi (Vigna unguiculata) contra o fungo fitopatogÃnico Lasiodiplodia theobromae. A proteÃna recombinante foi expressa em Pichia pastoris, coletada e purificada apÃs 72h de induÃÃo, utilizando cromatografia de afinidade em matriz de quitina. A quitinase foi eluÃda a partir da cromatografia de afinidade com Ãcido acÃtico a 0,1 M. Ensaio enzimÃtico foi realizado contra o substrato sintÃtico (quitina coloidal), a fim de determinar a atividade da proteÃna recombinante purificada. A quitinase apresentou atividade especÃfica de 5.637,32 U/mg de proteÃna. Testes biolÃgicos foram realizados. Nestes testes trÃs diferentes isolados de L. theobromae, identificados como CNPAT CCJ-127, CNPAT CCJ-166 e CNPAT CCJ-184, foram utilizados e os experimentos foram realizados em triplicata. Os isolados fÃngicos foram obtidos da coleÃÃo de trabalho do LaboratÃrio de Fitopatologia da Embrapa AgroindÃstria Tropical (Fortaleza-CE, Brasil). Em todos os ensaios biolÃgicos o fungicida Carbomax 500 SCÂ (Carbendazim), a uma concentraÃÃo de 2 mL/L, e Ãgua destilada estÃril foram utilizados como controles positivos e negativos, respectivamente. Um total de 50, 100 e 300 Âg de quitinase recombinante (rVuChi) foi utilizado em todos os testes. O primeiro ensaio foi baseado na metodologia de difusÃo em disco de papel de filtro em que foram investigados os efeitos da proteÃna sobre o crescimento do micÃlio, bem como a formaÃÃo de halo de inibiÃÃo sobre o crescimento micelial do fungo. O segundo ensaio foi baseado no ensaio de difusÃo em Ãgar. Fotografias foram usadas para registrar as observaÃÃes. A quitinase rVuChi mostrou efeito fungistÃtico variando de moderado a forte sobre o crescimento micelial de todos os isolados de L. theobromae, particularmente quando usada nas doses de 100 e 300 Âg, no ensaio de difusÃo em disco. CNPAT CCJ-127 foi o isolado mais resistente Ã aÃÃo fungistÃtica de rVuChi, como observado pelo menor impacto da proteÃna em seu crescimento micelial. No teste de difusÃo em Ãgar a quantidade de 300 Âg foi a mais efetiva, da mesma forma como observado para o de difusÃo em disco de papel de filtro. AlÃm disso, o efeito da proteÃna foi mais pronunciado nos isolados CNPAT CCJ-166 e CNPAT CCJ-184 e menos impactante no isolado CNPAT CCJ-127. A quitinase recombinante rVuCHi mostrou ser um inibidor do crescimento micelial de trÃs diferentes isolados de L. theobromae. Os efeitos fungistÃticos da proteÃna aqui descritos podem ser devido Ã sua capacidade de degradar quitina, um biopolÃmero estrutural que faz parte da parede celular de vÃrios fungos fitopatogÃnicos, incluindo L. theobromae. Entretanto, mais estudos precisam ser conduzidos para revelar os possÃveis mecanismos de aÃÃo de rVuChi sobre L. theobromae.<br>The aim of this work was to evaluate the biological activity of a recombinant chitinase (rVuChi) from cowpea (Vigna unguiculata) against the phytopathogenic fungus Lasiodiplodia theobromae. The recombinant protein was expressed in Pichia pastoris, collected and purified after 72h of induction, using a chitin affinity chromatography. The chitinase was eluted from the affinity chromatography using 0.1 M acetic acid. Enzymatic assay was performed against the synthetic substrate (colloidal chitin) in order to determine the activity of the purified recombinant protein. The chitinase displayed a specific activity of 5,637.32 U/mg of protein. Biological tests were performed. In these tests three different isolates of L. theobromae, identified as CNPAT CCJ-127, CNPAT CCJ-166 and CNPAT CCJ-184, were used and the experiments were performed on triplicate. The fungal isolates were obtained from the collection of work from the laboratory of plant pathology from the Embrapa AgroindÃstria Tropical (Fortaleza-CE, Brasil). In all biological assays the fungicide Carbomax 500 SCÂ (Carbendazim) at a concentration of 2 mL/L and sterile distilled water were used as positive and negative controls, respectively. A total of 50, 100 and 300 Âg of recombinant chitinase (rVuChi) was used in all tests. The first test was based on the disk diffusion methodology using filter paper in which the effects of the protein on the mycelium growth, as well as the formation of an inhibition zone on the fungal hyphae were investigated. The second test was based on the diffusion assay in agar. Photographs were used to register the observations. The rVuChi showed moderate to strong fungistatic activities on the mycelial growth of all L. theobromae isolates when used at 100 and 300 Âg in the disk diffusion assay. CNPAT CCJ-127 was the most resistant specimen to the rVuChi fungistatic action, as observed by the lower impact of the protein on it is mycelial growth. In the agar diffusion test the amount of 300 Âg was the most effective, as observed in the disk diffusion test. In addition, the effect of the protein was most pronounced on the isolates CNPAT CCJ-166 and CNPAT CCJ-184 and less impacting on CNPAT CCJ-127. The recombinant chitinase rVuCHi showed to be an inhibitor of the mycelial growth of three L. theobromae isolates. The fungistatic effects of the protein described here may be due to its ability to degrade chitin, a structural biopolymer that makes part of the cell wall of several phytopathogenic fungi, including L. theobromae. Once this is only a scientific speculation, more studies need to be made to definitely reveal the mechanism of action of rVuChi on L. theobromae.

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Lopes, Neto Antônio Viana. "Atividade fungistática de uma quitinase recombinante do feijão de corda [Vigna unguiculata (L.) (Walp.)] contra Lasiodiplodia theobromae Pat. (Griff. e Maubl.), agente causal da resinose do cajueiro (Anacardium occidentale L." reponame:Repositório Institucional da UFC, 2014. http://www.repositorio.ufc.br/handle/riufc/18857.

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LOPES NETO, Antônio Viana. Atividade fungistática de uma quitinase recombinante do feijão de corda [Vigna unguiculata (L.) (Walp.)] contra Lasiodiplodia theobromae Pat. (Griff. e Maubl.), agente causal da resinose do cajueiro (Anacardium occidentale L.). 2014. 57 f. Dissertação (Mestrado em Bioquímica)-Universidade Federal do Ceará, Fortaleza-CE, 2014.<br>Submitted by Eric Santiago (erichhcl@gmail.com) on 2016-07-08T13:45:57Z No. of bitstreams: 1 2014_dis_avlopesneto.pdf: 1483564 bytes, checksum: 0e776910b7f818898df5c2fc18bff4ec (MD5)<br>Approved for entry into archive by José Jairo Viana de Sousa (jairo@ufc.br) on 2016-08-02T20:18:18Z (GMT) No. of bitstreams: 1 2014_dis_avlopesneto.pdf: 1483564 bytes, checksum: 0e776910b7f818898df5c2fc18bff4ec (MD5)<br>Made available in DSpace on 2016-08-02T20:18:18Z (GMT). No. of bitstreams: 1 2014_dis_avlopesneto.pdf: 1483564 bytes, checksum: 0e776910b7f818898df5c2fc18bff4ec (MD5) Previous issue date: 2014<br>The aim of this work was to evaluate the biological activity of a recombinant chitinase (rVuChi) from cowpea (Vigna unguiculata) against the phytopathogenic fungus Lasiodiplodia theobromae. The recombinant protein was expressed in Pichia pastoris, collected and purified after 72h of induction, using a chitin affinity chromatography. The chitinase was eluted from the affinity chromatography using 0.1 M acetic acid. Enzymatic assay was performed against the synthetic substrate (colloidal chitin) in order to determine the activity of the purified recombinant protein. The chitinase displayed a specific activity of 5,637.32 U/mg of protein. Biological tests were performed. In these tests three different isolates of L. theobromae, identified as CNPAT CCJ-127, CNPAT CCJ-166 and CNPAT CCJ-184, were used and the experiments were performed on triplicate. The fungal isolates were obtained from the collection of work from the laboratory of plant pathology from the Embrapa Agroindústria Tropical (Fortaleza-CE, Brasil). In all biological assays the fungicide Carbomax 500 SC® (Carbendazim) at a concentration of 2 mL/L and sterile distilled water were used as positive and negative controls, respectively. A total of 50, 100 and 300 µg of recombinant chitinase (rVuChi) was used in all tests. The first test was based on the disk diffusion methodology using filter paper in which the effects of the protein on the mycelium growth, as well as the formation of an inhibition zone on the fungal hyphae were investigated. The second test was based on the diffusion assay in agar. Photographs were used to register the observations. The rVuChi showed moderate to strong fungistatic activities on the mycelial growth of all L. theobromae isolates when used at 100 and 300 µg in the disk diffusion assay. CNPAT CCJ-127 was the most resistant specimen to the rVuChi fungistatic action, as observed by the lower impact of the protein on it is mycelial growth. In the agar diffusion test the amount of 300 µg was the most effective, as observed in the disk diffusion test. In addition, the effect of the protein was most pronounced on the isolates CNPAT CCJ-166 and CNPAT CCJ-184 and less impacting on CNPAT CCJ-127. The recombinant chitinase rVuCHi showed to be an inhibitor of the mycelial growth of three L. theobromae isolates. The fungistatic effects of the protein described here may be due to its ability to degrade chitin, a structural biopolymer that makes part of the cell wall of several phytopathogenic fungi, including L. theobromae. Once this is only a scientific speculation, more studies need to be made to definitely reveal the mechanism of action of rVuChi on L. theobromae.<br>O objetivo deste trabalho foi avaliar a atividade biológica de uma quitinase recombinante (rVuChi) de feijão-caupi (Vigna unguiculata) contra o fungo fitopatogênico Lasiodiplodia theobromae. A proteína recombinante foi expressa em Pichia pastoris, coletada e purificada após 72h de indução, utilizando cromatografia de afinidade em matriz de quitina. A quitinase foi eluída a partir da cromatografia de afinidade com ácido acético a 0,1 M. Ensaio enzimático foi realizado contra o substrato sintético (quitina coloidal), a fim de determinar a atividade da proteína recombinante purificada. A quitinase apresentou atividade específica de 5.637,32 U/mg de proteína. Testes biológicos foram realizados. Nestes testes três diferentes isolados de L. theobromae, identificados como CNPAT CCJ-127, CNPAT CCJ-166 e CNPAT CCJ-184, foram utilizados e os experimentos foram realizados em triplicata. Os isolados fúngicos foram obtidos da coleção de trabalho do Laboratório de Fitopatologia da Embrapa Agroindústria Tropical (Fortaleza-CE, Brasil). Em todos os ensaios biológicos o fungicida Carbomax 500 SC® (Carbendazim), a uma concentração de 2 mL/L, e água destilada estéril foram utilizados como controles positivos e negativos, respectivamente. Um total de 50, 100 e 300 µg de quitinase recombinante (rVuChi) foi utilizado em todos os testes. O primeiro ensaio foi baseado na metodologia de difusão em disco de papel de filtro em que foram investigados os efeitos da proteína sobre o crescimento do micélio, bem como a formação de halo de inibição sobre o crescimento micelial do fungo. O segundo ensaio foi baseado no ensaio de difusão em ágar. Fotografias foram usadas para registrar as observações. A quitinase rVuChi mostrou efeito fungistático variando de moderado a forte sobre o crescimento micelial de todos os isolados de L. theobromae, particularmente quando usada nas doses de 100 e 300 µg, no ensaio de difusão em disco. CNPAT CCJ-127 foi o isolado mais resistente à ação fungistática de rVuChi, como observado pelo menor impacto da proteína em seu crescimento micelial. No teste de difusão em ágar a quantidade de 300 µg foi a mais efetiva, da mesma forma como observado para o de difusão em disco de papel de filtro. Além disso, o efeito da proteína foi mais pronunciado nos isolados CNPAT CCJ-166 e CNPAT CCJ-184 e menos impactante no isolado CNPAT CCJ-127. A quitinase recombinante rVuCHi mostrou ser um inibidor do crescimento micelial de três diferentes isolados de L. theobromae. Os efeitos fungistáticos da proteína aqui descritos podem ser devido à sua capacidade de degradar quitina, um biopolímero estrutural que faz parte da parede celular de vários fungos fitopatogênicos, incluindo L. theobromae. Entretanto, mais estudos precisam ser conduzidos para revelar os possíveis mecanismos de ação de rVuChi sobre L. theobromae.

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Books on the topic "Plant pathogenic fungus"

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1951-, Faeth Stanley H., ed. Ecology and evolution of the grass-endophyte symbiosis. Oxford University Press, 2009.

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International, C. A. B., ed. Fungal plant pathogens. CABI, 2012.

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Bolton, Melvin D., and Bart P. H. J. Thomma, eds. Plant Fungal Pathogens. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-501-5.

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Lane, C., P. Beales, and K. J. D. Hughes, eds. Fungal plant pathogens. CABI, 2012. http://dx.doi.org/10.1079/9781845936686.0000.

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Plant pathogenic fungi. J. Cramer, 1987.

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Prell, Hermann H., and Peter Day. Plant-Fungal Pathogen Interaction. Springer Berlin Heidelberg, 2001. http://dx.doi.org/10.1007/978-3-662-04412-4.

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Arya, A., and A. E. Perelló, eds. Management of fungal plant pathogens. CABI, 2010. http://dx.doi.org/10.1079/9781845936037.0000.

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Plant fungal pathogens: Methods and protocols. Humana Press, 2012.

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McKenzie, Eric H. C. Fungi, bacteria, and pathogenic algae on plants in American Samoa. South Pacific Commission, 1996.

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service), SpringerLink (Online, ed. Microbial Plant Pathogens-Detection and Disease Diagnosis: Fungal Pathogens, Vol.1. Springer Science+Business Media B.V., 2011.

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Book chapters on the topic "Plant pathogenic fungus"

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Charlton, Nikki D., Stellos M. Tavantzis, and Marc A. Cubeta. "Detection of Double-Stranded RNA Elements in the Plant Pathogenic Fungus Rhizoctonia solani." In Plant Pathology. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-59745-062-1_14.

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Ciuffetti, Lynda M., Viola A. Manning, Iovanna Pandelova, et al. "Pyrenophora tritici-repentis: A Plant Pathogenic Fungus with Global Impact." In Genomics of Plant-Associated Fungi: Monocot Pathogens. Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-662-44053-7_1.

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Scheel, Dierk, Karl Dietrich Hauffe, Willi Jahnen, and Klaus Hahlbrock. "Stimulation of Phytoalexin Formation in Fungus-Infected Plants and Elicitor-Treated Cell Cultures of Parsley." In Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-71652-2_31.

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Tegli, S., A. Scala, V. Goggioli, and C. Bonati. "Responses of Carnation Callus Cultures to Inoculation with F. Oxysporum f.sp. Dianthi and a Non Pathogenic Fungus." In Developments in Plant Pathology. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1737-1_107.

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Hohl, Hans R., and Sylvia Balsiger. "A Model System for the Study of Fungus — Host Surface Interactions: Adhesion of Phytophthora Megasperma to Protoplasts and Mesophyll Cells of Soybean." In Recognition in Microbe-Plant Symbiotic and Pathogenic Interactions. Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-71652-2_25.

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Vidal, Stefan. "Changes in suitability of tomato for whiteflies mediated by a non-pathogenic endophytic fungus." In Proceedings of the 9th International Symposium on Insect-Plant Relationships. Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-1720-0_61.

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Yue, Zhang, Yang Jing, Liu Lin, et al. "Analysis of Secretary Proteins in the Genome of the Plant Pathogenic Fungus Botrytis Cinerea." In Computer and Computing Technologies in Agriculture IV. Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-18333-1_27.

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Liao, Zhen, Kristian Persson Hodén, and Christina Dixelius. "Small talk and large impact: the importance of small RNA molecules in the fight against plant diseases." In RNAi for plant improvement and protection. CABI, 2021. http://dx.doi.org/10.1079/9781789248890.0086.

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Abstract This short and general chapter summarizes how plants and pathogens communicate using not only proteins for recognition and signal transduction or other metabolites but also RNA molecules where small RNAs with sizes between 21 to 40 nt are most important. These small RNAs can move between plants and a range of interacting pathogenic organisms in both directions, that is, a 'cross-kingdom' communication process. The first reports on RNA-based communications between plants and plant pathogenic fungi appeared about 10 years ago. Since that time, we have learnt much about sRNA biology in plants and their function in different parasitic organisms. However, many questions on the processes involved remain unanswered. Such information is crucial in order to sustain high crop production. Besides giving a brief background, we highlight the interactions between the potato late blight pathogen and its plant host potato.

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Liao, Zhen, Kristian Persson Hodén, and Christina Dixelius. "Small talk and large impact: the importance of small RNA molecules in the fight against plant diseases." In RNAi for plant improvement and protection. CABI, 2021. http://dx.doi.org/10.1079/9781789248890.0009.

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Abstract This short and general chapter summarizes how plants and pathogens communicate using not only proteins for recognition and signal transduction or other metabolites but also RNA molecules where small RNAs with sizes between 21 to 40 nt are most important. These small RNAs can move between plants and a range of interacting pathogenic organisms in both directions, that is, a 'cross-kingdom' communication process. The first reports on RNA-based communications between plants and plant pathogenic fungi appeared about 10 years ago. Since that time, we have learnt much about sRNA biology in plants and their function in different parasitic organisms. However, many questions on the processes involved remain unanswered. Such information is crucial in order to sustain high crop production. Besides giving a brief background, we highlight the interactions between the potato late blight pathogen and its plant host potato.

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Bennett, Laura, Artem Lysenko, Lazaros G. Papageorgiou, et al. "Detection of Multi-clustered Genes and Community Structure for the Plant Pathogenic Fungus Fusarium graminearum." In Computational Methods in Systems Biology. Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-33636-2_6.

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Conference papers on the topic "Plant pathogenic fungus"

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Veselova, S. V., T. V. Nuzhnaya, G. F. Burkhanova, and S. D. Rumyantsev. "Characteristics of dose-response dependence between zeatin and resistance of wheat plants to the pathogenic fungus Stagonospora nodorum." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.269.

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The effect of three concentrations of trans-zeatin on the indices of wheat plant resistance to S. nodorum was studied. Two concentrations of trans-zeatin showed a maximum increase in the resistance of wheat plants to S. nodorum.

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"Different resistance of bread wheat near-isogenic lines carrying various combinations of alleles of the Pp and Ba genes regulating the biosynthesis of anthocyanin to the pathogenic fungus Stagonosporа nodorum". У Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-214.

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Щербакова, Татьяна, Штефан Кручан, Борис Пынзару та Леонид Волощук. "Антагонизм новых изолятов грибов рода Trichoderma по отношению к патогену Alternaria Sp., выделенному из ореха грецкого". У International Scientific Symposium "Plant Protection – Achievements and Prospects". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2020. http://dx.doi.org/10.53040/9789975347204.39.

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The research is aimed at studying the antagonistic relationships of five isolates of the genus Trichoderma fungi in relation to the Alternaria sp. fungus. The pathogen was isolated from diseased walnut leaves. In the work was used the method of double cultures on agar media. As a result, 160 the inhibition rate of Alternaria pathogen by Trichoderma fungi was determined. For isolate №3 the rate was 90%, for isolate №2 - 85%, isolate №4 inhibited the pathogen by 75%, isolate №1 - by 65%. Isolate №5 and strains T.virens and T. lignorum suppressed the growth of Alternaria by 40-50%.

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Santos, Taís Araújo, Elza Thaynara Cardoso De Menezes Assis, Jocilene Dos Santos Pereira, and Letícia Maróstica De Vasconcelos. "A TECNOLOGIA CRISPR/CAS9 NA RESISTÊNCIA DE PLANTAS CONTRA PATÓGENOS FÚNGICOS." In I Congresso de Engenharia de Biotecnologia. Revista Multidisciplinar de Educação e Meio Ambiente, 2021. http://dx.doi.org/10.51189/rema/1372.

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Introdução: As plantas são suscetíveis a um grande número de patógenos, incluindo os fungos. Fitopatógenos fúngicos são responsáveis por inúmeras doenças, como ferrugem, oídio, podridão, entre outras. Diferentes estratégias têm sido desenvolvidas para aumentar a resistência fúngica em espécies de plantas com base no conhecimento atual dos mecanismos moleculares envolvidos na interação planta-patógeno. As tecnologias de edição de genoma progrediram rapidamente e se tornaram as ferramentas genéticas mais utilizadas para o melhoramento de plantas. Entre essas, temos a aplicação do sistema formado por repetições palindrômicas curtas, interespaçadas e regularmente agrupadas (CRISPR), e sua proteína associada-9 (Cas9). Objetivo: Apresentar a tecnologia de edição de genoma CRISPR/Cas9 com foco na sua aplicação para o aumento da resistência de plantas á patógenos fúngicos. Metodologia: A pesquisa foi realizada nas bases de dados: PubMed e Scopus. Para alcançar o máximo de precisão na estratégia de busca, utilizou-se os descritores: “plant”, “pathogen”, “fungi or fungus”, “CRISPR”. Resultados: A maioria dos trabalhos envolvendo a resistência de plantas contra patógenos fúngicos estavam relacionados como a capacidade do sistema CRISPR/Cas9 em induzir mutagênese direcionada, com competência em silenciar genes implicados na interação planta-fungo. Foi possível observar vários estudos onde os genes de suscetibilidade da planta hospedeira foram inativados, pois eram necessários para o ciclo de vida do patógeno, demonstrando que a tecnologia é aplicável à resistência a doenças fúngicas em plantas, pois o silenciamento de um determinado gene na planta pode resultar em uma suscetibilidade no fungo. Conclusão: Os resultados demonstram a aplicação vantajosa do sistema CRISPR/Cas9 para o melhoramento de culturas no que diz respeito à resistência a patógenos. O aumento dessa resistência possui um papel importante, pois os fungos fitopatogênicos representam uma ameaça para a produção e o rendimento das safras agrícolas.

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Mohamed, Ibtihal, and Talaat Ahmad. "Screening Of Some Qatari Plant Extracts For Anti Fungal Activities Of Plant Pathogenic Fungi." In Qatar Foundation Annual Research Conference Proceedings. Hamad bin Khalifa University Press (HBKU Press), 2014. http://dx.doi.org/10.5339/qfarc.2014.eesp0735.

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Sarvarova, E. R., E. A. Cherepanova, and I. V. Maksimov. "Antifungal activity of lipopeptides from endophytic strains of the genus Bacillus sp. against the fungus Stagonospora nodorum (Berk.)." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.216.

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The direct antibiotic effect of lipopeptides from four endophytic strains on the germination of spores of the pathogenic fungus Stagonospora nodorum (Berk.) was found and the minimum inhibitory concentration (MIC) of these lipopeptides was determined.

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Zhang, Wei. "Epigenetic regulation of effector gene expression in fungal plant pathogen." In ASPB PLANT BIOLOGY 2020. ASPB, 2020. http://dx.doi.org/10.46678/pb.20.1372292.

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Merkel, K. A., and E. P. Vibe. "THE PREVALENCE OF INFECTIOUS LODGING OF SEEDLINGS OF COMMON PINE IN AUTUMN SOWING AT THE NURSERY OF SFNR" ERTIS ORMANY»." In STATE AND DEVELOPMENT PROSPECTS OF AGRIBUSINESS Volume 2. DSTU-Print, 2020. http://dx.doi.org/10.23947/interagro.2020.2.38-40.

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Phytopathological studies of the state of shoots of common pine (Pinus sylvestris L.) of autumn sowing when grown in protected soil with the use of Agrotex covering material were carried out. The results of survey showed that when using fertilizers and soil activators-nitrogen, phosphorus, boric acid, EM EKO KZ Soil, EM EKO KZ soil (NPK) and humus, there is a drop in seedlings from 7.5 to 32.6%. The main reason for the death of shoots was the development and spread of infectious pathogens of seedlings. The species composition of pathogenic microflora in diseased plants is represented by a group of soil fungi belonging to the anamorphic division-Fusarium, Alternaria. Evaluation of the experience of introducing substances into the soil during autumn sowing showed that their use, as a rule, does not affect the resistance of plants to infectious pathogens, with the exception of boric acid, which has fungicidal activity.

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Belyakova, N. V., E. A. Vorobyova, and V. A. Sivolapov. "MOLECULAR-GENETIC ANALYSIS OF PHYTOPATHOGENS IN STANDS OF THE VORONEZH REGION." In Modern machines, equipment and IT solutions for industrial complex: theory and practice. Voronezh State University of Forestry and Technologies named after G.F. Morozov, Voronezh, Russia, 2021. http://dx.doi.org/10.34220/mmeitsic2021_29-33.

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This paper presents the results of DNA diagnostics of phytopathogens in the Voronezh region. DNA diagnostics was carried out step by step: isolation of total DNA from the sample by CTAB method, amplification of marker regions of phytopathogenic organisms using primers ITS1 and ITS4, electrophoretic separation of the obtained amplicons in 2% agarose gel followed by staining with ethidium bromide, determination of the nucleotide sequence of the amplified loci ABI Prism 310. The study identified the following plant diseases: Sphaeropsis sapinea, Rhizoctonia solani, Cladosporium herbarum. Along with this, we identified the Neocatenulostroma pathogen, which had not previously been found in the territories under its jurisdiction. This disease cannot be determined by phenological signs. The degree of infection by pathogens ranged from 15 to 40%. At present, the problem of protecting plants from diseases is especially urgent. It has been established that the greatest damage to forestry activities is caused by fungal and infectious diseases. At the same time, among phytopathogens, about 97% are fungal infections, 2% are bacterial and 1% are viral.

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Vasilchenko, N. G., A. V. Gorovtsov, V. A. Chistyakov, and M. S. Mazanko. "BACTERIA OF THE ORDER BACILLALES AS PROMISING ANTAGONISTS OF FUSARIUM PATHOGENS AND THEIR IMPACT ON WINTER WHEAT PLANTS." In INNOVATIVE TECHNOLOGIES IN SCIENCE AND EDUCATION. DSTU-Print, 2020. http://dx.doi.org/10.23947/itno.2020.324-327.

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The possibility of using bacteria of the order Bacillales as agents of biological control of phytopathogenic fungi of the genus Fusarium was studied. In the work, 28 soil samples were studied, from which antagonist bacterial strains were isolated. Antagonism was detected by cultivating a pasteurized soil suspension with a culture of the fungus Fusarium graminearum on wort agar. In the course of this work, 1040 antagonist bacterial strains were isolated. Subsequently, the influence of the selected strains of microorganisms on the germination winter wheat seeds and several other morphometric parameters was studied.

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Reports on the topic "Plant pathogenic fungus"

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Harms, Nathan, Judy Shearer, James Cronin, and John Gaskin. Geographic and genetic variation in susceptibility of Butomus umbellatus to foliar fungal pathogens. Engineer Research and Development Center (U.S.), 2021. http://dx.doi.org/10.21079/11681/41662.

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Large-scale patterns of plant invasions may reflect regional heterogeneity in biotic and abiotic factors and genetic variation within and between invading populations. Having information on how effects of biotic resistance vary spatially can be especially important when implementing biological control because introduced agents may have different Impacts through interactions with host-plant genotype, local environment, or other novel enemies. We conducted a series of field surveys and laboratory studies to determine whether there was evidence of biotic resistance, as foliar fungal pathogens, in two introduced genotypes (triploid G1, diploid G4) of the Eurasian wetland weed, Butomus umbellatus L. in the USA. We tested whether genotypes differed in disease attack and whether spatial patterns in disease incidence were related to geographic location or climate for either genotype. After accounting for location (latitude, climate), G1 plants had lower disease incidence than G4 plants in the field (38% vs. 70%) but similar pathogen richness. In contrast, bioassays revealed G1 plants consistently received a higher damage score and had larger leaf lesions regardless of pathogen. These results demonstrate that two widespread B. umbellatus genotypes exhibit different susceptibility to pathogens and effectiveness of pathogen biological controls may depend on local conditions.

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Konopka, James B., Arturo Casadevall, John W. Taylor, Joseph Heitman, and Leah Cowen. One Health: Fungal Pathogens of Humans, Animals, and Plants. American Society for Microbiology, 2019. http://dx.doi.org/10.1128/aamcol.18oct.2017.

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Nelson, Linda S., Judy P. Shearer, and Michael D. Netherland. Integrated Fluridone-Fungal Pathogen Treatment of Four Submersed Plants. Defense Technical Information Center, 1998. http://dx.doi.org/10.21236/ada363773.

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Kingsley, Mark T. Nucleic Acid-Based Detection and Identification of Bacterial and Fungal Plant Pathogens - Final Report. Office of Scientific and Technical Information (OSTI), 2001. http://dx.doi.org/10.2172/781863.

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Kingsley, Mark T. Nucleic Acid-Based Detection and Identification of Bacterial and Fungal Plant Pathogens - Final Report. Office of Scientific and Technical Information (OSTI), 2001. http://dx.doi.org/10.2172/965696.

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Freedman, Jan E., Michael J. Grodowitz, Robin Swindle, and Julie G. Nachtrieb. Potential Use of Native and Naturalized Insect Herbivores and Fungal Pathogens of Aquatic and Wetland Plants. Defense Technical Information Center, 2007. http://dx.doi.org/10.21236/ada471715.

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