Academic literature on the topic 'Plant pigments – Analysis'
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Journal articles on the topic "Plant pigments – Analysis"
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Patterson, G. I., L. J. Harris, V. Walbot, and V. L. Chandler. "Genetic analysis of B-Peru, a regulatory gene in maize." Genetics 127, no. 1 (January 1, 1991): 205–20. http://dx.doi.org/10.1093/genetics/127.1.205.
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Abstract The B locus in maize is required for the accumulation of anthocyanin pigments. Numerous B alleles have been described: each determines a particular pattern of pigment synthesis with respect to the tissues that are pigmented and the time during development that pigment synthesis begins. We report here a genetic analysis of one B allele, B-Peru, which regulates synthesis of pigments in both kernel and plant tissues. We used stocks with active Mutator transposable elements to produce eight mutations in B-Peru. All eight alter pigment synthesis in all the kernel and plant tissues pigmented by B-Peru, suggesting that each mutation has disrupted a region of the gene required for expression in all tissues. Six of the mutations cause a colorless phenotype, while two cause a reduction in pigment in both kernel and plant tissues. Four of the mutations are unstable, and four are stable upon self-pollination. Multiple independent revertants were isolated from each unstable allele. DNA blot analysis demonstrated that all eight mutants are the result of insertions within an approximately 5-kb region that encodes the B-Peru transcript. One of the four unstable alleles contains a Mu element, Mu1.7. Two others contain insertions related to the Spm family of transposable elements. Thus, our Mutator stocks also contained active transposable elements from the unrelated Spm family. Our experiments suggest that the B-Peru allele is not complex, but contains a single coding region that regulates pigment synthesis in multiple tissues.
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Cuadra, Pedro, and Jeffrey B. Harborne. "Changes in Epicuticular Flavonoids and Photosynthetic Pigments as a Plant Response to UV-B Radiation." Zeitschrift für Naturforschung C 51, no. 9-10 (October 1, 1996): 671–80. http://dx.doi.org/10.1515/znc-1996-9-1012.
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Treatment of Gnaphalium vira-vira plants with UV-B radiation caused changes in plant growth and in plant chemistry. The leaf surface contained two O-methylated flavones, araneol and 7-O-methylaraneol. HPLC analysis showed that 20 days of UV-B radiation increased the synthesis of 7-O-methylaraneol at the expense of araneol. Spectrophotometric analysis of the photosynthetic pigments showed that UV-B radiation also increases the pigment content in treated plants. Another U V alteration is epidermal hair damage, as observed in SEM pictures of treated leaves. This combination of physiological and phytochemical effects may be interpreted as a plant response to UV-B stress
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Varzakas, T., and S. Kiokias. "HPLC Analysis and Determination of Carotenoid Pigments in Commercially Available Plant Extracts." Current Research in Nutrition and Food Science Journal 4, no. 1 (February 24, 2015): 01–14. http://dx.doi.org/10.12944/crnfsj.4.special-issue1.01.
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Carotenoids are a class of natural pigments, familiar to all through the orange-red colours, which are added to many manufactured foods in the forms of natural extracts or as pure chmically syntesided compounds in order to give the desired colouring properties. Recent evidence has correlated a diet rich in certain carotenoids with an increased protection against cardiovascular diseases or even certain types of cancer. In addition, on going research examines potential protective effects of these natural compounds in food systems e.g. oils and food emulsions. In order to understand and optimise the activity of the natural extracts when used in vitro or in vivo , it is really important first to to identify those active coumpounds that account for any any dietary and functional role. This paper focuses on the HPLC analysis of certain plant extracts aiming at the quantification and identification of the present natural carotenoid pigments. Saponification prior to HPLC Analysis was found to be successful in order to remove unwanted lipids and esters and thereby facilitate the idientification of main carotenoid pigments in the natural extracts. By use of UV spectroscopy, the total carotenoid content of each natural extract was also roughly estimated based on their extinction coefficient at the λmax of the main carotenoid pigment following dilution at the appropriate solvent. The identification and quantification of carotenoids in plant extract is considerd as essential in the further developmnt and optimisation of their functional uses as additives and dietary supplements.
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Oksana, Belous, Klemeshova Kristina, and Pashchenko Olga. "Comparative analysis of photosynthetic indicators in freesia hybrids on the Black sea coast of Krasnodar region." Horticultural Science 44, No. 2 (May 11, 2017): 99–104. http://dx.doi.org/10.17221/189/2015-hortsci.
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The paper discusses some features in Freesia refracta pigment system and possible use of this option as a plant diagnostic characteristic on the resistance in limiting environments. The study included freesia cultivars and hybrid forms selected in the Russian Research Institute of Horticulture and Subtropical Crops, which had different flowering terms. A detailed analysis was provided on any changes in the photosynthetic pigments content during the growing process. There are parameters of slow chlorophyll fluorescence induction (vitality index, ratio of photosynthetic activity and estimated coefficient of photosynthetic activity) in the leaves of different cultivars and hybrid forms. In order to determine the dependences between photosynthetic pigments content and parameters of slow chlorophyll fluorescence induction in freesia cultivars and hybrid forms pair correlation coefficient; it was calculated between these features among all hybrids and cultivars in general. The relationship recorded between optical indicators, and structural features of the pigment system will allow us to develop a method of rapid diagnosis of photosynthetic activity in freesia on the Black Sea coast of the Krasnodar region.
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Çakmakçi, Emrah, Ozan Deveoglu, Ahmed Muhammed, Ali Fouad, Emine Torgan, and Recep Karadag. "HPLC-DAD analysis of Thymus serpyllum based natural pigments and investigation of their antimicrobial properties." Pigment & Resin Technology 43, no. 1 (December 20, 2013): 19–25. http://dx.doi.org/10.1108/prt-07-2012-0045.
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Purpose – In this study, it was aimed to investigate the antibacterial properties of natural pigments prepared from Thymus serpyllum. Design/methodology/approach – Al (III), Fe (II), Sn (II) and Cu (II) complexed natural pigments were obtained by using a precipitation method and the main constituents in the pigments were identified with HPLC-DAD. Also FTIR analysis was performed for further structural characterization. Moreover, the thermal stability and thermal degradation properties of the pigments were analyzed by thermogravimetric analyses (TGA). The antimicrobial activity of the thyme plant-extracted pigments was evaluated by measuring the minimal inhibitory concentration. Findings – Apigenin and luteolin flavones were detected as the main components of the natural dyes. Thermal degradation behaviour of the pigments was determined by means of TGA. All pigments showed high char yields and it was attributed to the high complexation between the metal and the ligand species. The antimicrobial activity of the thyme plant-extracted pigments was measured and it was found that all pigments had high antimicrobial activity. Aluminum-thymus pigments showed the highest antimicrobial efficiency among other pigments used in this study. Originality/value – The obtained pigments have high antimicrobial activities, and therefore, they can be used for the production of antimicrobial textiles. Furthermore, Thymus-based natural pigments might have potential applications in coating, paint, plastic industries, etc.
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Komyshev, E. G., M. A. Genaev, and D. A. Afonnikov. "Analysis of color and texture characteristics of cereals on digital images." Vavilov Journal of Genetics and Breeding 24, no. 4 (July 2, 2020): 340–47. http://dx.doi.org/10.18699/vj20.626.
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The color of the grain shell of cereals is an important feature that characterizes the pigments and metabolites contained in it. The grain shell is the main barrier between the grain and the environment, so its characteristics are associated with a number of important biological functions: moisture absorption, grain viability, resistance to pre-harvest germination. The presence of pigments in the shell affects various technological properties of the grain. Color characteristics, as well as the appearance of the grain shell are an important indicator of plant diseases. In addition, the color of the grains serves as a classifying feature of plants. Genetic control of the color formation of both grains and other plant organs is exerted by genes encoding enzymes involved in the biosynthesis of pigments, as well as regulatory genes. For a number of pigments, these genes are well understood, but for some pigments, such as melanin, which causes the black color of grains in barley, the molecular mechanisms of biosynthesis are still poorly understood. When studying the mechanisms of genetic control of grain color, breeders and geneticists are constantly faced with the need to assess the color characteristics of their shell. The technical means of addressing this problem include spectrophotometers, spectrometers, hyperspectral cameras. However, these cameras are expensive, especially with high resolution, both spatial and spectral. An alternative is to use digital cameras that allow you to get high-quality images with high spatial and color resolution. In this regard, recently, in the field of plant phenotyping, methods for evaluating the color and texture characteristics of cereals based on the analysis of two-dimensional images obtained by digital cameras have been intensively developed. This mini-review is devoted to the main tasks related to the analysis of color and texture characteristics of cereals, and to methods of their description based on digital images.
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Weber, Courtney A., and William Boone. "467 Mapping Anthocyanin Pathway Genes in Raspberry." HortScience 35, no. 3 (June 2000): 474D—474. http://dx.doi.org/10.21273/hortsci.35.3.474d.
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The role of plant pigments in human health has been under intense scrutiny recently. Anthocyanin pigments have been shown to be powerful antioxidants and may contribute to other areas of human health. In red and black raspberry, Rubus idaeus and Rubus occidentalis, respectively, no less than eight different anthocyanin pigments have been identified. However, the genetics controlling the presence and ratios of the different pigments is poorly understood. Various researchers have identified four loci that impart fruit pigment deficiencies and three loci that affect the pigment ratios. The underlying gene function of these loci is not known. Efforts are under way to map two pigment deficiency loci in red raspberry using bulked segregant analysis. Screening with 800 random primers has produced two markers with >90% and two with >80% correlation to one loci. For the other loci, 10 markers with >80% correlation have been identified. Mapping is ongoing with the first linkage map for raspberry to be presented. Populations to test allelism between sources of pigment deficiency are being evaluated for further mapping of loci of the anthocyanin production pathway. Data on cloning of genes in the anthocyanin pathway based on database sequences with degenerative primers for further elucidation a anthocyanin production in raspberry will be presented.
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Ashokhan, Sharmilla, Sujatha Ramasamy, Saiful Anuar Karsani, Rashidi Othman, and Jamilah Syafawati Yaacob. "Analysis of bioactive pigments in coloured callus of Azadirachta indica for possible use as functional natural colourants." Pigment & Resin Technology 48, no. 1 (January 7, 2019): 9–19. http://dx.doi.org/10.1108/prt-11-2017-0095.
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Purpose The purpose of this study is to evaluate the content of bioactive pigments in coloured callus of Azadirachta indica and to understand the correlation between the callus colours with their bioactive constituents, antioxidant properties and cytotoxicity. These assessments will yield valuable insight into the use of in vitro-derived pigments for possible use as functional natural colourants. Design/methodology/approach In this study, the authors have successfully developed a protocol to produce leaf-derived callus of various colours with enhanced content of bioactive pigments in A. indica through plant tissue culture. Comparative analysis of the pigments content (chlorophyll, carotenoid, phenolics and anthocyanins) in the coloured callus was conducted, followed by evaluation of its bioactive properties. The antioxidant properties against 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radicals, ferric reducing antioxidant power and cytotox activities of the coloured callus extracts were also reported. Findings Callus of various colours were successfully produced in A. indica through plant tissue culture, and their valuable pigment content and bioactivity were evaluated. The green callus contained the highest amount of anthocyanin, followed by brown and cream callus. The total anthocyanin contents in both the green and brown callus was more than two-fold higher than that in cream callus. Contrasting observation was obtained for total phenolic content (TPC), where the TPC of cream callus was significantly higher than that in brown callus. Nevertheless, the green callus also exhibited the highest TPC. Green callus also contained the highest amount of total chlorophyll and carotenoid, as well as exhibited the highest antioxidant potential, and was found to be the only extract with active cytotox activity against SKOV-3 cells. Correlation analysis revealed that the excellent bioactivity exhibited by the coloured extracts was strongly correlated with the bioactive pigments present in the callus. Research limitations/implications The major bioactive compounds identified in the methanolic extracts of A. indica coloured callus are anthocyanins, phenolics, chlorophylls and carotenoids. Future research work should include improvements in the extraction and identification methods, which may lead to detection and determination of other compounds that could attribute to its bioactivity, to complement the findings of the current study. Practical implications This analysis provides valuable information on the application of plant tissue culture as an alternative source for sustainable production of major pigments with medicinal benefits in A. indica for possible use as functional natural colourants. Originality/value A comparative study on bioactive pigment production in coloured callus from A. indica leaves and its antioxidant potential and cytotoxicity is original. To the best of the authors’ knowledge, this is the first report detailing a comparative evaluation on the production of coloured callus in A. indica and its relative biochemical composition and bioactive properties.
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Redžić, Sulejman, Nizama Hodžić, and Mijat Tuka. "Plant pigments (antioxidants) of medicinal plants Malva Sylvestris l. and Malva Moschata l. (Malvaceae)." Bosnian Journal of Basic Medical Sciences 5, no. 2 (May 20, 2005): 53–58. http://dx.doi.org/10.17305/bjbms.2005.3284.
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Qualitative-quantitative structure of plant pigments in wild plants Malva silvestrs L. and Malva moschata L. (Malvaceae), which were collected in 20 locations in Sarajevo area and surroundings, was tested during spring and summer in 2003. Acetone extracts of both categories were made and rising paper-chromatography done for the purpose of qualitative analysis. Quantitative analysis was done by spectrophotometry. Chlorophyll a, chlorophyll b and xanthophylls presence was confirmed by separation of pigments from acetone extract of these plant species. Spectrophotometric analysis of acetone extracts showed these results (given in mg/L): chlorophyll a 2,386, chlorophyll b 0,332 and carrotenoides 1,037. Data given in mg/g dry substance are: chlorophyll a 1,193x10(-2), chlorophyll b 1,66x10(-3), and carrotenoides 5,185x10(-3). Pigments structure (in mg/L) in species Malva moschata is 1,6 for chlorophyll; 1,419 for chlorophyll b; and 0,364 for carrotenoides. Data given in mg/g are: chlorophyll a 8x10(-3), chlorophyll b 7,09x10(-3), and carrotenoides 1,82x10(-3). Considering that species Malva moschata L. grows on ecologically clear soils as opposed to well-known medicinal species Malvasylvestris L., and considering the production of phytomass, phytochemical structure and pharmacological influence it can be considered very medical and be given advantage over this wider spread category.
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Watson, J. F., and I. L. Goldman. "Inheritance and Linkage Analysis of a Blotchy Root Pigment Phenotype in Red Beet." HortScience 32, no. 3 (June 1997): 454A—454. http://dx.doi.org/10.21273/hortsci.32.3.454a.
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The primary pigments in red beet are the betalains, which include the red-violet betacyanins and the yellow betaxanthins. The recent adoption of betalain pigments from red beet as an alternative to synthetic food dyes has heightened interest in genetic modification of pigment production. Dominant alleles at two tightly-linked loci (R and Y) condition production of betalain pigment in the beet plant; however, several alleles at the R locus influence pigment amount and distribution. A mutant phenotype, characterized by irregular sectors of blotchy red and white root color, arose spontaneously in the beet breeding nursery. The blotchy mutant plant was used in crosses with non-blotchy inbred lines to characterize its inheritance. Chisquare goodness-of-fi t tests of segregation data in backcross and F2 generations for several genetic backgrounds did not deviate from the hypothesis that a single recessive gene controls the blotchy phenotype. Linkage analysis was conducted to determine if the blotchy phenotype was conditioned by a new locus or an allele at a previously described locus. Our data indicate the bl gene resides at a newly described locus linked to R and Y. Maximum likelihood estimation revealed a linkage distance between R and Y of 8.95 ± 0.49 cM. The linkage distance between R and BL was calculated at 13.99 ± 1.18 cM, and the overall linkage between Y and BL was determined to be 28.8 ± 4.2 cM. Our data suggest the RYBL genomic region plays a critical role in the genetic control of betalain biosynthesis.
Dissertations / Theses on the topic "Plant pigments – Analysis"
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Pflanz, Michael. "A methodical approach for non-destructive estimation of plant pigments by means of remission spectroscopy applied in fruit and vegetable analyses." Doctoral thesis, Humboldt-Universität zu Berlin, Landwirtschaftlich-Gärtnerische Fakultät, 2014. http://dx.doi.org/10.18452/17074.
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Anbaubegleitende spektral-optische Analysen direkt an der Pflanze haben zunehmend Bedeutung für die gartenbauliche Produktion. Veränderungen individueller Pflanzeninhaltsstoffe können mit Hilfe optischer Sensoren unmittelbar und beliebig oft erfasst werden, wodurch Pflanzenreaktionen auf veränderte Umgebungs- und Kulturbedingungen instrumentell messbar sind. Im Konzept eines Präzisionsgartenbau könnten diese zusätzlichen Informationen adaptiven Kulturmaßnahmen dienen und zur effizienten Nutzung von Ressourcen in der Produktion von Obst und Gemüse beitragen. Die wellenlängenspezifische Lichtabsorption pflanzlicher Gewebe ermöglicht zerstörungsfreie Reflexions- oder Transmissionsmessungen und die Adressierung von Pigmentgruppen im ultravioletten und sichtbaren Bereich des elektromagnetischen Spektrums. Auf Grund veränderlicher Gehalte während des Wachstum und der Entwicklung pflanzlicher Gewebe, sind Pigmente zudem wichtige Indikatoren für den physiologischen Zustand von Obst und Gemüse. Präzise zerstörungsfreie Spektralanalysen werden allerdings durch komplexe optische Eigenschaften biologischen Materials, besonders im Hinblick auf die Reifeentwicklung von Obst und Gemüse erschwert. Zur Lösung dieser Problematik wurde ein neuer iterativer Ansatz (iMLR) entwickelt, der überlagerte in-situ und in-vivo Spektren individueller Pigmente aus einem Summenmessspektrum separiert. Dieser Algorithmus wurde in eine eigenständige Applikation überführt. Eine Datenbank enthält spektrale Signaturen von Chlorophyll a und b, Lycopin, β-Carotin, α-Carotin, Lutein und Violaxanthin. Obwohl die spektrale Analyse einzelner Pigmente durch optische Störgrößen in komplex aufgebauten biologischen Geweben und Pigmentgemischen erschwert ist, konnte ein neuer Ansatz erarbeitet und validiert werden. Diese Methode minimiert spektrale Überlagerung von in-situ und in-vivo aufgezeichneten Messsummensignalen und analysiert Pigmentgehalte in Farbstoffgemische zuverlässig.Spectral measurements on plants have already been introduced in practice through extensive research and through the recent increase in the availability of low-cost devices. It can be expected that optical sensor systems may contribute to an economic and sustainable use of natural resources as a part of the concept for precision horticulture. In terms of phytomonitoring approaches, such technologies which address variable amounts of individual chromophoric plant components become more important. Their wavelength-selective light absorption makes pigments specifically responsive to reflection or transmission recordings in the ultraviolet and visible range of the electromagnetic spectrum. Additionally, pigments serve as indicators for physiological stages of leaf and fruit. Consequently, the instrumental recording of variable pigment contents has high potential with regards to dynamic plant-adapted processes during the production of fruit and vegetables. Solving the known issues of non-destructive spectroscopy, a new approach was figured out in the present work to obtain a more precise analysis of individual pigment contents, which vary during the cultivation of horticultural crops. The tool is based on an iterative algorithm (iMLR), that separates coinciding in-situ and in-vitro spectra from sum signals of individual pigments. Finally, the algorithm was integrated into a stand-alone application containing a library of chlorophyll a and b, as well as signatures of lycopene, β-carotene, α-carotene, lutein and violaxanthin. It can be pointed out that individual pigment compositions are suitable indicators of the physiological stage of horticultural products. However, the spectral analysis of single pigment levels is challenging due to complex interactions of coinciding absorption and diffuse light scattering in natural pigment mixtures or in fruit extracts. From this, an improved method for the reliable decomposition of spectral signals was developed.
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Pflanz, Michael [Verfasser], Uwe [Akademischer Betreuer] Schmidt, Manuela [Akademischer Betreuer] Zude, and Thomas [Akademischer Betreuer] Rath. "A methodical approach for non-destructive estimation of plant pigments by means of remission spectroscopy applied in fruit and vegetable analyses / Michael Pflanz. Gutachter: Uwe Schmidt ; Manuela Zude ; Thomas Rath." Berlin : Humboldt Universität zu Berlin, Landwirtschaftlich-Gärtnerische Fakultät, 2014. http://d-nb.info/106350323X/34.
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Damaraju, Sridevi Verfasser], Bernhard [Akademischer Betreuer] [Grimm, Heiko [Akademischer Betreuer] Lockstein, and Kurt [Akademischer Betreuer] Zoglauer. "Analysis of proteins involved in chlorophyll catabolism : the impact of chlorophyllase and water soluble chlorophyll protein on the reduction of undesirable pigments in crop plants / Sridevi Damaraju. Gutachter: Bernhard Grimm ; Heiko Lockstein ; Kurt Zoglauer." Berlin : Humboldt Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, 2011. http://d-nb.info/1015081312/34.
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Aoun, Michel. "Action du cadmium sur les plants de moutarde indienne [Brassica juncea (L.) Czern] néoformés à partir de couches cellulaires minces et issus de semis. Analyses physiologiques et rôle des polyamines." Phd thesis, Université de Bretagne occidentale - Brest, 2008. http://tel.archives-ouvertes.fr/tel-00385705.
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La phytoremédiation constitue une nouvelle technologie permettant de dépolluer les sols contaminés par l'utilisation de plantes. Parmi les différents aspects possibles de cette méthode, figure la phytoextraction basée sur l'absorption et l'accumulation du polluant dans les parties aériennes. Pour être efficace, il est nécessaire de disposer de plantes présentant une biomasse élevée. L'objectif de ce travail visait à sélectionner des variétés de moutarde indienne (Brassica juncea L.) tolérantes et accumulatrices de cadmium.La première partie du travail a consisté à mettre au point et à optimiser une méthode de régénération in vitro de plantes de moutarde indienne, à partir de couches cellulaires minces transversales (CCMTs) (influence de l'organe utilisé, de AgNO3 et de la benzylaminopurine). La régénération a été réalisée en appliquant une pression de sélection par le cadmium pour modifier la tolérance au métal.
La deuxième partie aborde l'effet des traitements par le cadmium : in vitro et en serre, sur le développement des plants. Une analyse des perturbations physiologiques et biochimiques observées a permis d'évaluer la tolérance des plants vis à vis du cadmium et indiquent que les plantes néoformées en présence de cadmium mettent en place un système d'exclusion du métal.
Dans la troisième partie, pour compléter l'étude précédente sur des plantes néoformées, l'effet du cadmium a été testé sur des plantes de B. juncea directement issues de semis. Plusieurs paramètres physiologiques et biochimiques, caractéristiques des stress, ont été étudiés (activité gaïacol peroxydase, peroxydation des lipides, pigments, acides aminés libres, proline glucides, polyamines libres et conjuguées). En raison de leurs propriétés anti-oxydantes, une attention particulière a été portée aux polyamines dont l'application exogène permet d'envisager son utilisation pour améliorer la capacité d'accumulation du cadmium par les plantes.
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Ramonetha, Thata Golden. "Synthetic and spectroscopic studies of 6-substituted chromone derivatives." Diss., 2015. http://hdl.handle.net/11602/772.
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Gordon, Allen Tauya. "Synthetic studies and biological evaluation of chromone - 3 - carbaldehydes." Diss., 2018. http://hdl.handle.net/11602/1214.
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MSc (Chemistry)Department of Chemistry
Chromones are well known naturally occurring heterocyclic compounds with oxygen as a heteroatom. Chromones are also one of the major classes of naturally occurring compounds, and the interest in their chemistry is due to their wide range of their biological activity. In this study, three classes of target compounds were synthesized through three different pathways. The first pathway, chromone-3-carbaldehyde analogues were afforded in good to excellent yield followed by the oxidation thereof to 4-oxo-4H-chromene-3-carboxylic acids. A series of chromone-3-carboxamides was obtained from corresponding 4-oxo-4H-chromene-3-carboxylic acid via the in situ generation of the corresponding acid chloride in good yield. The second class of compounds were achieved by reacting corresponding chromone-3-carbaldehyde analogues with thiazolidine-2,4-dione to afford 5-((4-oxo-4H-chromen-3-yl)methylene)thiazolidine-2,4-dione analogues. The third class of compounds followed the same reaction pathway as the second class of compounds from corresponding 8-allyl-chromone-3-carbaldehyde analogues to afford 5-((8-allyl-4-oxo-4H-chromen-3-yl)methylene)thiazolidine-2,4-dione analogues in good yield. Compounds were characterized by 1D NMR (1H, 13C and DEPT), 2D NMR (COSY, HSQC and HMBC), IR and elemental analysis (CHN analysis). Selected synthesized chromone derivatives were evaluated in vitro for two biological assays; namely trypanocidal activity and cytotoxicity. Among all tested compounds, 41A, 55B and 63D displayed promising trypanocidal activity by reducing the percentage parasite viability to 0.61, 0.15 and 0.21 respectively. These results were further substantiated by their IC50 values 4.3, 1.3 and 1.9 μg/mL respectively. Compounds 41B and 59A also showed significant trypanocidal activity, however it was below the positive control. Compounds 41A and 55B displayed cytotoxicity against the HeLa cells whilst compound 63D displayed no toxicity against the HeLa cells.
NRF
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CHEN, WEN-TZU, and 陳文子. "Natural Pigment Development from Natural Plants(I ) Phytochemical, free radical scavenging and hair coloring analysis from herbs." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/9v67g5.
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碩士佛光大學
未來與樂活產業學系
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In 2011, the Taiwan elderly population has reached 10.8%, which was showing the aging time has come. In order to maintain a youthful appearance, "hair-dyeing" has become the necessities of life for mature population. Many studies have confirmed the synthesis of artificial coloring hazardous to human health. 2016 BMC Cancer latest research paper pointed out: compared to the hair-not-dyed people, the hair-dyed ones suffering from prostate cancer has the ratio of 1.15 times higher than the normal people. That indicates that the use of hair dye will increase the risk of prostate cancer. For this reason, today's hair preparations or cosmetic products should be made toward safe, healthy direction. Phytochemicals (phytochemicals) exists only in plants, where polyphenols have been proved to have antioxidant activity, prevention of chronic diseases and health promotion. Taiwan is located in the subtropical zone where the plants are rich in resources and the people use herbal treatment for a long time. This study focused on traditional natural pigment of plants as the basis for selecting or tonic with antioxidant effects of Chinese herbal medicine materials, extraction and refining and correlation analysis. Collecting 50 kinds of herbal and extracting them with water and alcohol have a total of 100 kinds of extracts, following the analysis of the extracts were: (I) chloride, iron color reaction analysis, the results showed positive reactions were a total of 11 kinds of extracts were found to contain more phenolic factors. (II) Do the following chemical analysis and activity measurement: (i) Chemical analysis (1) contains the alkaloid as CY-008 and 028; (2) contain flavonoids as CY-034, 035, and 045; (3) contain anthraquinones as CY-012, 028, and 046; (4) containing triterpenoids as CY -039; containing steroid component CY-045. (ii) determination of tryptic activity by removing two kind of the alkaloid extract containing a total of nine kinds of drugs then extracts (1) antioxidant capacity is measured DPPH radical application of the clear results nine kinds of Chinese herbal extract (1mg / 100ul) of the radical scavenging rate between 83% to 6%, in order, is CY-045 > 035 > 034 > 012 > 046 > 013 > 022 > 026 > 039; (2) Folin-Ciocalteu (FC) analysis of the reaction to Gallic acid as a standard, total phenol content of Gallic acid equivalent amount of 9.064 ~ between 0.569 ug / m. Experimental results, the analysis of the data, and the 5 designs of people favorite colors--black and brown, by testing on the wool hair to determine the effects: (1) expose to sunlight, showed that hair piece dyed consolidated above the good color stability. (2) The same clinical hair dyeing procedure showed that five kinds of formulations were deepened with the increasing number of hair coloring, that indicates these five new recipes has the potential development for new hair dyeing product, also has the effect of anti-oxygen and improve health. This study further designed and developed a new natural hair dye so that people both dye their hair and promote the effectiveness of health at the same time to the pursuit of aesthetics of life and health of the target.
Books on the topic "Plant pigments – Analysis"
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Golubkina, Nadezhda, Elena Kekina, Anna Molchanova, and Sergey Nadezhkin. Antioxidants of plants and methods of their definition. ru: INFRA-M Academic Publishing LLC., 2020. http://dx.doi.org/10.12737/1045420.
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The monograph presents the most simple and widely used methods for determining the most important of plant antioxidants: vitamin C, polyphenols, carotenoids, capsaicin, and belinovich photosynthetic pigments, flavonoids, anthocyanins, alkaloids, tannins, and minerals antioxidant: selenium and iodine. Special attention is paid to methods of extraction of antioxidants, providing maximum extraction of antioxidants from plant material, and the correct selection of the most appropriate method of analysis of one or another component. Provides detailed information developed by the authors method of using thin layer chromatography to assess the carotenoid composition of tomatoes and peppers.
The data presented here include results of research conducted on the basis of FICO, as well as the latest developments of foreign scientists devoted to natural antioxidants and methods of their determination. Presented in this monograph methodology was successfully tested in the laboratory and analytical Department of PNCO in 2012-2018.
For students and teachers and all interested in horticulture and agriculture.
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Letswaart, Th. Estimating phytoplankton biomass by means of microscopical counts and HLPC pigment analysis: A comparison. Amsterdam: Association of River Waterworks, RIWA, 2000.
Find full textBook chapters on the topic "Plant pigments – Analysis"
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"Drugs Containing Pigments." In Plant Drug Analysis, 281–89. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-00574-9_13.
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schoefs, Benoît. "Plant Pigments: Properties, Analysis, Degradation." In Advances in Food and Nutrition Research, 41–91. Elsevier, 2005. http://dx.doi.org/10.1016/s1043-4526(05)49002-6.
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Galbraith, David W., and Jan BartoŠ. "Flow Cytometry and Cell Sorting in Plant Biotechnology." In Flow Cytometry for Biotechnology. Oxford University Press, 2005. http://dx.doi.org/10.1093/oso/9780195183146.003.0021.
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Higher plants comprise approximately 250,000 described species and represent a critical component of the planetary biomass. They contribute functions essential for life, of which the most important is photosynthesis, as it provides the means for conversion of incident solar radiation into biomass accumulation, as well as the oxygen required by aerobic life forms. Fixed carbon in the form of carbohydrate provides the basis of the food chain, and metabolic interconversions within plants provide a variety of essential dietary factors. Plants also provide biomass in the form of structural materials and are the source of many natural products with important biomedical properties. As a consequence, considerable scientific interest is invested in determining the molecular mechanisms underlying plant growth, development, metabolism, and responses to biotic and abiotic stresses. Investment has also been made in developing tools and resources for biological investigations using plants. Notable advances include the development of genetics, of means for transformation using defined DNA sequences, and most recently, of the entire nuclear genome sequences of two plant species (Arabidopsis thaliana and Oryza sativa). On the basis of information of this type and that from other sources, it is evident that higher plants share many features with other eukaryotic organisms. Shared features can be observed at many levels; for example, the overall method of construction of cells, in which a bilamellar plasma membrane separates the cytoplasm from the external milieu and provides primary homeostatic regulation. Eukaryotic cells of different kingdoms share organelles, as well as overall regulatory mechanisms. Shared, or highly similar, protein sequences are observed, and they perform similar functions as enzymes, regulatory molecules, or structural components . Higher land plants have evident differences from other eukaryotes. They contain unique classes of organelles primarily devoted to energy capture from sunlight (plastids and peroxisomes). Of these, chloroplasts contain highly fluorescent pigments devoted to photosynthesis, which, particularly chlorophyll, provide unique and powerful signals that can be employed for flow cytometric analysis. Higher plants are also essentially immobile in the sporophytic stage and hence must be capable of responding to changes in environmental conditions and to biotic attack.
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Polivova, Maria, and Anna Brook. "Detailed Investigation of Spectral Vegetation Indices for Fine Field-Scale Phenotyping." In Vegetation Index and Dynamics [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96882.
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Spectral vegetation indices (VIs) are a well-known and widely used method for crop state estimation. These technologies have great importance for plant state monitoring, especially for agriculture. The main aim is to assess the performance level of the selected VIs calculated from space-borne multispectral imagery and point-based field spectroscopy in application to crop state estimation. The results obtained indicate that space-borne VIs react on phenology. This feature makes it an appropriate data source for monitoring crop development, crop water needs and yield prediction. Field spectrometer VIs were sensitive for estimating pigment concentration and photosynthesis rate. Yet, a hypersensitivity of field spectral measures might lead to a very high variability of the calculated values. The results obtained in the second part of the presented study were reported on crop state estimated by 17 VIs known as sensitive to plant drought. An alternative approach for identification early stress by VIs proposed in this study is Principal Component Analysis (PCA). The results show that PCA has identified the degree of similarity of the different states and together with reference stress states from the control plot clearly estimated stress in the actual irrigated field, which was hard to detect by VIs values only.
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Tan, Suat Hian, Nyuk Ling Ma, and Wan Nurul Hidayah Wan Anuar. "Analysis of the Comparative Study on Flower Pigments with Phenylalanine Ammonia-Lyase Activity in Orchid Plants." In Challenging Issues on Environment and Earth Science Vol. 3, 79–89. Book Publisher International (a part of SCIENCEDOMAIN International), 2021. http://dx.doi.org/10.9734/bpi/ciees/v3/7922d.
Full textConference papers on the topic "Plant pigments – Analysis"
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VAGUSEVICIENĖ, Ilona, Sonata KAZLAUSKAITĖ, Aiste JUCHNEVICIENĖ, Asta BYLAITE, and Audrone ŽEBRAUSKIENĖ. "COMPARISON OF PHOTOSYNTHETIC PARAMETERS IN DIFFERENT WHEAT (TRITICUM AESTIVUM L.) VARIETIES." In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.064.
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Dynamics of photosynthesis pigments in the leaves of different varieties of winter wheat during the vegetation period is analyzed in the paper. The accumulation of pigments in the plant depends on the physiological activity, growth and development of the plant, therefore the composition and content of photosynthesis pigments chlorophyll a, b and carotenoids reflect the general condition of the plant. The ratio of chlorophyll a / b for normal photosynthesis activity in the leaves of the plant should be at least 1:3. The object of the research is different varieties of winter wheat (Triticum aestivum L.) - 'Artist', 'Edvin', 'Skagen', 'Bertold' and 'Viola'. Field experiment was carried out at the Experimental Station of Aleksandras Stulginskis University in 2015-2016. Soil type was identified as IDg8 - k (LVg - p - w - cc) - shallow calcareous luvisol (Calc (ar) i - Epihypogleyic Luvisols). Agrochemical parameters of the soil were determined using accepted analytical methods. The content of photosynthesis pigments (chlorophyll a, b and carotenoids) in green leaf mass was determined in 96% ethyl alcohol extract applying spectrophotometric Wettstein method, “Genesys” 6 spectrophotometer. The photosynthesis productivity (Fpr) was calculated according to the formula: Fpr = 2 (M2-M1) / (L1 + L2) T. The accuracy of the data analysis was estimated according to the standard measurement deviation from the mean. The highest content of photosynthesis pigments has been accumulated by winter wheat variety 'Skagen'. The best result has been observed at the end of nodding stage. A lower content of photosynthesis pigments has been found in the leaves of 'Edvin', 'Viola' and 'Artist' varieties. The highest photosynthesis productivity of all winter wheat varieties has been recorded at the end of nodding stage, and decrease of photosynthesis productivity has been observed since milk maturity stage.
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JUCHNEVIČIENĖ, Aistė, and Ilona VAGUSEVIČIENĖ. "THE DYNAMICS OF PHOTOSYNTHETIC PIGMENTS IN WINTER WHEAT LEAVES WHEN USING NITROGEN FERTILISERS." In Rural Development 2015. Aleksandras Stulginskis University, 2015. http://dx.doi.org/10.15544/rd.2015.033.
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The paper investigates the effect of nitrogen fertilisers on the amount of photosynthetic pigments in winter wheat leaves. The research was carried out in the period between 2012 and 2013 at the Experimental Station of Aleksandras Stulginskis University in carbonate shallow gleyic leached soil, (Calc(ar)i-Epihypogleyic Luvisol). The object of investigation: winter wheat cultivars ‘Zentos’ and ‘Ada’. Granular superphosphate (P60) and potassium chloride (K60) fertilisers were spread during sowing, while amonium nitrate (N60) was used in tillering time (BBCH 23–25), after the vegetative growth had resumed. Additionally, the plants were treated with foliar fertiliser urea solution: N30, N40 at booting stage (BBCH 34–36) and N15, N30 at milk ripening stage (BBCH 71–74). After the analysis of the data, it was established that additional fertilization with N30 and N40 fertiliser application rates at later stages of plant development stimulated the accumulation of photosynthetic pigments and prolonged the period of active photosynthesis. Irrespective of treatment with nitrogen fertilisers, genetic properties of the cultivar also had influence on the accumulation of the pigments. Wheat cultivar ‘Zentos’ tended to accumulate larger amounts of pigments. The highest amounts of pigments were found at the beginning of milk ripening stage before additional treatment with N15, N30 fertiliser application rates.
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Turbina, I. N., and G. M. Kukurichkin. "ANALYSIS OF THE PIGMENT STATUS OF SOME SPECIES OF GENUS ACER L. USING THE PROGRAM STATISTICA." In Prirodopol'zovanie i ohrana prirody: Ohrana pamjatnikov prirody, biologicheskogo i landshaftnogo raznoobrazija Tomskogo Priob'ja i drugih regionov Rossii. Izdatel'stvo Tomskogo gosudarstvennogo universiteta, 2020. http://dx.doi.org/10.17223/978-5-94621-954-9-2020-56.
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The present paper is aimed at the analysis of adaptation mechanisms of five species of the genus Acer L. in the culture of the botanical garden.With this view we studied the indices of the pigment complex (nitrogen balance - Nbi, flavonols - Flv and chlorophyll - Ch) in the assimilating organs of plants. It was revealed that the pigment composition of plant leaves indicates their relatively high physiological status. An increase in biochemical parameters (Nbi,Chl, Flv) was identified by the end of summer in comparison with the beginning of summer period, which testifies to the formation of the protective function of the plant in the pre-winter period. The following statistical methods were used for test results interpretation: Shapiro – Wilk tests, Mann – Whitney, Wilcoxon, Kruskal-Wallisand criteria.
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Gonome, Hiroki, Mehdi Baneshi, Atsuki Komiya, and Shigenao Maruyama. "Control of Radiative Properties of Coatings Pigmented With Fe2O3 Nanoparticles." In ASME/JSME 2011 8th Thermal Engineering Joint Conference. ASMEDC, 2011. http://dx.doi.org/10.1115/ajtec2011-44622.
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This study describes nanoparticles pigmented coatings used in controlling the radiative properties of surfaces exposed to sunlight. An optimization method that embraces both thermal and aesthetic requirements has been proposed. The proposed coatings maximize the reflectivity of the near infrared (NIR) region to reduce thermal heating, while for aesthetic appeal they minimize the visible (VIS) reflected energy. This spectral behavior can be achieved by controlling the size and concentration of pigment particles and coating thickness. In this study, both experimental and numerical approaches are applied on Fe2O3 pigmented coating samples with 0.2 μm and 1 μm of average particle size and different particle concentrations and coating thicknesses. For numerical part the radiation analysis using the Radiation Element Method by Ray Emission Model (REM2) in a one dimensional parallel plane model is conducted. From the numerical results, it is shown that the optimum size of Fe2O3 particles for our desired spectral behavior is about 0.8 μm. The experimental results also show that the samples made from 1 μm particles have better performance for our objective.
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"Study of melanin and anthocyanin biosynthesis regulation in barley grain by transcriptomic analysis of near-isogenic lines with different pigment composition." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-215.
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Mantha, S., L. Mongeau, and T. Siegmund. "Dynamic Digital Image Correlation of a Dynamic Physical Model of the Vocal Folds." In ASME 2005 International Mechanical Engineering Congress and Exposition. ASMEDC, 2005. http://dx.doi.org/10.1115/imece2005-81457.
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An experimental study of the vibratory deformation of the human vocal folds was conducted. Experiments were performed using model vocal folds [1, 2], Fig. 1, made of silicone rubber implemented into an air supply system, Fig. 2. The material used to cast the model is an isotropic homogeneous material, [3] with a tangent modulus E=5 kPa at ε = 0, i.e. elastic properties similar to those of the human vocal fold cover [4]. The advantages of the use of model larynx systems over the use of excised larynges include easy accessibility to fundamental studies of the vocal fold vibration without invasive testing. Acoustic analysis of voice or electroglottography provide certain insight into voice production processes but optical techniques for the study of vocal fold vibrations have drawn considerable attention. Videoendoscopy, stroboscopy, high-speed photography, and kymography have shown to provide a visual impression of vocal fold dynamics but are limited in providing insight into the fundamental deformation processes of the vocal folds. Quantitative measures of deformation have been conducted through micro-suture techniques but are invasive and allows for measurements of only view image points. Laser triangulation is non-invasive but is limited to only one local measurement point. Here, digital image correlation technique with the software VIC 3D [5] is applied. For the experimental set-up see Fig. 2. The analysis consists of (1) stereo correlation to obtain in-plane displacements and (2) stereo triangulation step to obtain out-of-plane deformation. For the stereo correlation images of the object at two different stages of deformation are compared. A point in the image of the undeformed object is matched with the corresponding point in the deformed stage. “Subsets” of digital images are traced via their gray value distribution from the undeformed reference image to the deformed image. The uniqueness of the matching is enabled by the creation of a speckle pattern on the object’s surface. Here, a white pigment is mixed into the silicone rubber and subsequently black enamel paint is sprayed onto the superior surface of the vocal folds. The stereo triangulation requires two images of the object at each stage of deformation. These are obtained in a single CCD frame by placing a beam splitter in the optical axis between camera and object. These images provide a “left” and “right” view of the model larynx. Thus, the deformed shape of the vocal folds can be obtained. The method allows for noninvasive measurement of the full-field displacement fields. Images of the superior surface of the model larynx are obtained by the use of a high speed digital camera with a frame rate of 3000 frames per second allowing for more than 30 image frames for each vibration cycle. For the 3D digital image correlation analysis two images of the object are obtained for each time instance as a beam splitter is placed in the optical axis between the camera and the model larynx. Phonation frequencies and onset pressure are given in Fig. 3, showing that the model larynx behavior is close to actual physiological data. Figs 4(a) and (b) provide superior views of the model larynx at maximum glottal opening and at glottal closure, respectively. As one example of measured strain fields, Figs 5(a) and (b) depict the distributions of the transverse strain component, on the glottal surface in a contour plot on the deformed superior surface. The knowledge of the distribution of this strain component is relevant to the assessment of the impact of vocal fold collision on potential tissue damage. In the position of maximum opening the vocal folds are deformed by a combination of a bulging-type deformation and the opening movement. At this time instance, the transverse strains at the medial surface are found to be negative, an indication of Poisson’s deformation. During the closing stage, vocal folds collide and simultaneously a mode 3 vibration pattern emerges. Closure of the glottal opening is not complete and two incomplete closure areas are formed during the closure stage. These open areas are located at the anterior and posterior ends of the model larynx, see Fig. 4(b). The finding of this type of incomplete closure is agreement with both actual glottal measurements [6] and 3D finite element simulations of [7]. Transverse strains during that stage are now positive and considerably larger that during the opening stage. Finally, Fig. 6 depicts the time evolution of the out of plane displacements along the medial surface for the closing phase and Fig. 7 depicts the maximum values of the longitudinal strain (at the coronal section of the medial surface) in dependence of the flow rate. These examples of measurements indicate that the DIC method is promising for studies of vocal fold dynamics.
Reports on the topic "Plant pigments – Analysis"
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Chen, Chen, Peng Chen, Xia Liu, and Hua Li. Combined 5-Fluorouracil and Low Molecular Weight Heparin for the Prevention of Postoperative Proliferative Vitreoretinopathy in Patients with Retinal Detachment. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, August 2021. http://dx.doi.org/10.37766/inplasy2021.8.0117.
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Review question / Objective: The aim of this meta-analysis is to evaluate the efficacy and safety of intraoperative infusion of combined 5-fluorouracil and low molecular weight heparin (LMWH) for the prevention of postoperative proliferative vitreoretinopathy in patients with retinal detachment. Condition being studied: Postoperative proliferative vitreoretinopathy (PVR) is the primary cause of failure of retinal reattachment surgery. 5-fluorouracil (5-FU) inhibits the proliferation of fibroblasts, and suppresses collagen contraction. On the other hand, heparin reduces fibrin exudation, and inhibits the adhesion and migration of retinal pigment epithelial cells. We conduct this comprehensive literature search and meta-analysis to address whether intraoperative infusion of combined 5-FU and LWMH improves the primary success rate of pars plana vitrectomy, as well as reduces postoperative PVR. Our study aims to provide clinical evidence for retinal surgeons concerning their choice of intraoperative medication.