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Journal articles on the topic 'Plant propagation – In vitro'

Author: Grafiati

Published: 4 June 2021

Last updated: 7 February 2022

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1

Kanwar J, K., and S. Kumar. "In vitro propagation of Gerbera: A Review." Horticultural Science 35, No. 1 (2008): 35–44. http://dx.doi.org/10.17221/651-hortsci.

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Gerbera has gained popularity in the past few years in many countries of the world and it is in great demand in the floral industry as cut flower as well as potted plant due to its beauty, colour, long vase life, and ability to rehydrate after long transportation. The most commercial cultivars are propagated through vegetative means by multiplication through divisions of clumps; however, the multiplication by this method is too slow to be commercially viable. To commercialize this crop and to meet the growing demand for planting material, tissue and organ culture techniques are being used as a

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2

Werbrouck, S. P. O., H. Dhuyvetter, R. M. Pérez, N. Topoonyanont, and P. C. Debergh. "PLANT PROPAGATION IN VITRO: HORMONAL INTERACTIONS." Acta Horticulturae, no. 560 (October 2001): 377–81. http://dx.doi.org/10.17660/actahortic.2001.560.71.

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3

Dubchak, Marcela, Olga Sultanova, and Viktor Bondarchuk. "Propagation of phytosanitary clones by in vitro culture." BIO Web of Conferences 34 (2021): 03003. http://dx.doi.org/10.1051/bioconf/20213403003.

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This article presents the process of accelerated reproduction of healthy clones of grapes, including the following stages: growing young shoots of the original plants of clones, introducing tops into in vitro culture, microclonal cuttings, adaptation of microplants to ex vitro culture, transplanting into cassettes with a soil substrate, transferring plants to a greenhouse for growing to the condition of vegetative seedlings and planting in a pre-propagation mother stock. For the successful implementation of each of the above operations in the SPIHVFT, a Cultural Complex has been equipped, cons

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4

Gupta, Pratibha, and Pratibha Singh. "In vitro Propagation of Gymnemasylvestre R. Br. (Retz.) – An Important Antidiabetic Medicinal Plant." International Journal of Scientific Research 3, no. 5 (2012): 31–34. http://dx.doi.org/10.15373/22778179/may2014/10.

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5

Sakr, Salwa, Saad Melad, M. El-Shamy, and Asmaa Elhafez. "In vitro Propagation of Polyscias fruticosa Plant." International Journal of Plant & Soil Science 3, no. 10 (2014): 1254–65. http://dx.doi.org/10.9734/ijpss/2014/8225.

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6

Ma, Yan, David H. Byrne, and Jing Chen. "Propagation of rose speciesin vitro." In Vitro Cellular & Developmental Biology - Plant 32, no. 2 (1996): 103–8. http://dx.doi.org/10.1007/bf02823139.

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7

Purohit, S. D., and G. Kukda. "In vitro propagation ofWrightia tinctoria." Biologia plantarum 36, no. 4 (1994): 519–26. http://dx.doi.org/10.1007/bf02921172.

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8

Markovic, Marija, Mihailo Grbic, and Matilda Djukic. "The use of in vitro culture in dianthus propagation." Bulletin of the Faculty of Forestry, no. 107 (2013): 141–62. http://dx.doi.org/10.2298/gsf1307137m.

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Today, in vitro culture is of the great importance in both scientific investigation of under-researched plant species and plant production. In this paper, a review of development and methods of in vitro culture is presented. The main principles are given and the most commonly used methods are described. Special attention was paid to the propagation of Dianthus spp. Tissue culture of commercially important taxa is described in detail, and the review of propagation of other decorative Dianthus spp. that can be used as ornamental plants is also given.

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9

Anuradha, S. K. Sehrawat, Anil K. Poonia, Subhash Kajla, and Sandhya Bhat. "Production of strawberry plant by in vitro propagation." Research on Crops 17, no. 3 (2016): 545. http://dx.doi.org/10.5958/2348-7542.2016.00091.7.

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10

Drewes, F. E., and J. van Staden. "In vitro propagation of Galtonia species." South African Journal of Botany 59, no. 4 (1993): 457–58. http://dx.doi.org/10.1016/s0254-6299(16)30720-7.

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11

Koetle, M. J., J. F. Finnie, and J. Van Staden. "In vitro propagation of Dierama erectum." South African Journal of Botany 75, no. 2 (2009): 408. http://dx.doi.org/10.1016/j.sajb.2009.02.070.

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12

Peron, J. Y., and E. Regnier. "In vitro propagation of Crambe maritima." Canadian Journal of Botany 65, no. 1 (1987): 72–75. http://dx.doi.org/10.1139/b87-010.

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A method for rapid micropropagation of sea kale (Crambe maritima L.) was developed. Petiole explants placed in vitro on a medium containing 0.5 mg/L indoleacetic acid (IAA), 6.0 mg/L kinetin, and 1.5 mg/L benzylaminopurine developed callus within 15 days and shoots within 28 days. Nearly four adventitious shoots could be developed within 3 weeks by placing the initial shoot on media without IAA. To develop roots, the shoots were then transferred to the basal medium containing 0.1 to 1.0 mg/L indolbutyric or α-naphthaleneacetic acid. Rooted plantlets were obtained within 2 or 3 weeks. After an

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13

Debnath, Samir C. "Propagation ofVaccinium in Vitro." International Journal of Fruit Science 6, no. 2 (2007): 47–71. http://dx.doi.org/10.1300/j492v06n02_04.

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14

Vinterhalter, Branka, Dijana Milošević, Teodora Janković, Jelena Milojević, and Dragan Vinterhalter. "In vitro propagation of Gentiana dinarica Beck." Open Life Sciences 7, no. 4 (2012): 690–97. http://dx.doi.org/10.2478/s11535-012-0059-7.

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AbstractGentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l−1 BA and 0.1 mg l−1 NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition

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15

Rout G, R., A. Mahato, and K. Senapati S. "In vitro clonal propagation of Nyctanthes arbortristis Linn. − a medicinal tree." Horticultural Science 34, No. 2 (2008): 84–89. http://dx.doi.org/10.17221/1852-hortsci.

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Rapid shoot multiplication of Nyctanthes arbortristis was achieved from axillary meristems on Murashige and Skoog (MS) basal medium supplemented with 1.0&minus;1.5 mg/l 6-benzyladenine (BA), 50 mg/l adenine sulfate (Ads) and 3% (m/v) sucrose. Inclusion of indole-3-acetic acid (IAA) in the culture medium along with BA + Ads promoted a higher rate of shoot multiplication. Maximum mean number of microshoots per explant (6.65) was achieved on the MS medium supplemented with 1.5 mg/l BA, 50 mg/l Ads and 0.1 mg/l IAA after 4 weeks of culture. The elongated shoots rooted within 13 to 14 days on &

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16

Purohit, S. D., K. Tak, and G. Kukda. "In vitro propagation ofBoswellia serrata Roxb." Biologia plantarum 37, no. 2 (1995): 335–40. http://dx.doi.org/10.1007/bf02913237.

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17

Purohit, S. D., K. Tak, and G. Kukda. "In vitro propagation ofBoswellia serrata Roxb." Biologia plantarum 37, no. 3 (1995): 335–40. http://dx.doi.org/10.1007/bf02913975.

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18

Tùng, Hoàng Thanh, Luan Quoc Vu, and Nhut Duong. "SOME TECHNIQUES IN MICROPROPAGATION AND BREEDING OF Paphiopedilum spp." Vietnam Journal of Science and Technology 58, no. 4 (2020): 393. http://dx.doi.org/10.15625/2525-2518/58/4/14779.

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Paphiopedilum orchids are one of the most popular and rare orchid genera sold and exhibited as pot plants and cut flowers. Their wild populations are under the threat of extinction as a result of over-collection and loss of suitable habitats. Reduction in their commercial value through large-scale propagation in vitro is a preferable option to reduce pressure from illegal collection, to attempt at meeting commercial needs and to re-establish these threatened orchid species back into the wild. Although they are commercially propagated via seed germination in vitro, Paphiopedilum are considered

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19

Stojakowska, Anna, and Janusz Malarz. "In vitro propagation of lnula royleana DC." Acta Societatis Botanicorum Poloniae 73, no. 1 (2011): 5–8. http://dx.doi.org/10.5586/asbp.2004.001.

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A micropropagation method, through axillary shoot proliferation, was elaborated for <em>Inula royleana </em>DC. (Asteraceae), a medicinal plant native of Himalaya. Primary explants (cotyledonary node explants) and secondary explants (node explants of in vitro regenerated shoots) of the plant, inoculated on MS medium supplemented with 0.1 μM NAA and 5.0 μM kinetin, regenerated 3.4 ± 1.2 and 5.1 ± 1.9 axillary shoots per explant, respectively. The regenerated shoots were easily rooting and adapting to growth in soil.

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20

Hamza, A., O. Abd El-Kafie, A. Helaly, and M. EL-Mongy. "IN VITRO PROPAGATION METHODES OF SNAPDRAGON (Antirrhinum majus.L) PLANT." Journal of Plant Production 4, no. 11 (2013): 1621–37. http://dx.doi.org/10.21608/jpp.2013.74484.

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21

Tejovathi, Gudipati. "In vitro propagation of endangered medicinal plant–Commiphora wightii." Indian Journal of Science and Technology 4, no. 11 (2011): 1537–41. http://dx.doi.org/10.17485/ijst/2011/v4i11.15.

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22

Uysal, Hüseyin. "In vitro propagation of black cumin (Nıgella satıva L.) plants." Genetika 53, no. 1 (2021): 295–303. http://dx.doi.org/10.2298/gensr2101295u.

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This study was carried out to determine in vitro development using Black cumin leaf and stem explants. ?ameli black cumin variety was used as plant material. Five different nutrient mediums (1. LS2.5, 2. MS, 3. MS + 0.5 mg.l-1 IAA, 4. MS + 0.5 mg.l-1 BAP, 5. MS + 0.5 mg.l-1 IAA + 0.5 mg.l-1 BAP) containing 30 g sugar were used in this study. As a result of the research, 100% callus formation was detected in the stem explants cultured in the number 1 and number 5 mediums. These were followed by stem explants cultured in medium 4 with a success rate of 96%. Of this rate, 66% was shoot formation,

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23

Pickens, Kimberly A., Jan Wolf, James M. Affolter, and Hazel Y. Wetzstein. "322 In Vitro Propagation of the Ornamental Bromeliad, Tillandsia eizii." HortScience 35, no. 3 (2000): 447E—447. http://dx.doi.org/10.21273/hortsci.35.3.447e.

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Many bromeliad species indigenous to the rain forests of Central and South America are threatened because of over-collection and habitat destruction. Studies were conducted to develop propagation protocols for Tillandsia eizii, a rare ornamental bromeliad of ceremonial significance to the Highland Maya communities in Chiapas, Mexico. We anticipate using in vitro propagation for the conservation of this species with the potential of utilizing bromeliads as an alternative and sustainable forest resource. Protocols were developed for the sterilization and germination of axenic seed. Seedling grow

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24

Chan, Charlotte R., and Robert D. Marquard. "Accelerated Propagation of Chionanthus virginicus via Embryo Culture." HortScience 34, no. 1 (1999): 140–41. http://dx.doi.org/10.21273/hortsci.34.1.140.

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Traditional seed propagation (warm/cold stratification) was compared to embryo culture of Chionanthus virginicus L. to determine if germination could be promoted and time necessary to produce a sizable plant could be reduced. Embryos of C. virginicus were extracted from immature fruit collected 9, 16, and 23 Aug. 1995 and grown in vitro on Anderson's rhododendron medium. They germinated in 4 weeks and were transferred ex vitro to flats. Mature fruit from the same source were grown simultaneously using warm/cold stratification. The two groups were evaluated periodically over a 2-year period for

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25

Mejía-Muñoz, J. M., S. González-Castillo, R. Mora-Aguilar, and J. E. Rodríguez-Pérez. "PROPAGACIÓN in vitro DE PAPA RATONA (Oxalis tuberosa Mol)." Revista Chapingo Serie Horticultura XII, no. 2 (2006): 231–37. http://dx.doi.org/10.5154/r.rchsh.2005.06.029.

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26

Kane, Michael E., Greg L. Davis, Dennis B. McConnell, and Jennifer A. Gargiulo. "In vitro propagation of Cryptocoryne wendtii." Aquatic Botany 63, no. 3-4 (1999): 197–202. http://dx.doi.org/10.1016/s0304-3770(99)00006-6.

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27

Pinheiro, Marcos Vinícius Marques, Daniele Cristina Fontana, Jullie dos Santos, Matheus Milani Pretto, Gabrieli Cristina Vitalli de Azevedo, and Denise Schmidt. "In vitro propagation of lemon verbena: a plant native of South America." Acta Scientiarum. Biological Sciences 41 (December 10, 2019): e47105. http://dx.doi.org/10.4025/actascibiolsci.v41i1.47105.

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In vitro propagation increases the supply and commercialisation of products of interest. For this, optimising the growing conditions and the composition of the culture medium is crucial to benefit the full development of the plants. Thus, the objective was to evaluate the in vitro propagation of Aloysia triphylla on different culture media, with varying agar and sucrose concentrations. The experiment was conducted as a completely randomised design, 3×3×3 factorial scheme, with three culture media (MS, JADS and WPM), three sucrose concentrations (8, 10 and 12 g L-1) and three agar concentration

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28

Goyali, Juran C., Abir U. Igamberdiev, and Samir C. Debnath. "Morphology, phenolic content and antioxidant capacity of lowbush blueberry (Vaccinium angustifolium Ait.) plants as affected by in vitro and ex vitro propagation methods." Canadian Journal of Plant Science 93, no. 6 (2013): 1001–8. http://dx.doi.org/10.4141/cjps2012-307.

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Goyali, J. C., Igamberdiev, A. U. and Debnath, S. C. 2013. Morphology, phenolic content and antioxidant capacity of lowbush blueberry ( Vaccinium angustifolium Ait.) plants as affected by in vitro and ex vitro propagation methods. Can. J. Plant Sci. 93: 1001–1008. The lowbush blueberry (Vaccinium angustifolium Ait.), a commercially important crop in eastern Canada and the United States of America, is native to North America. It is one of the richest sources of antioxidant compounds and has been reported to be a potential component in reducing the incidence of cancers and cardiovascular disease

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29

Jha, Timir B., Sumita Jha, and Sudripta Das. "In vitro propagation of cashewnut." Plant Cell Reports 15, no. 8 (1996): 615–19. http://dx.doi.org/10.1007/s002990050085.

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30

McCulloch, Steve. "1053 GENETIC STABILITY OF MICROPROPAGATED PLANTS." HortScience 29, no. 5 (1994): 579d—579. http://dx.doi.org/10.21273/hortsci.29.5.579d.

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Briggs Nurseries, Inc. has used micropropagation as method of vegetative propagation for over 20 years. Genetic stability and uniformity of plants that are produced and sold is of the utmost concern to the commercial plant propagator. Genetic stability may be accomplished by ensuring that all shoots formed in vitro are of axillary origin and by reducing shoot proliferation rates through the use of lower cytokinin concentrations in the culture medium. Excision and removal of callus during transfer is also necessary to ensure that shoots develop from axillary buds. Various factors that may influ

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31

Kuluev, B. R. "Darkening of Plant Tissues during in vitro Cultivation and Methods for its Prevention." Biotekhnologiya 36, no. 2 (2020): 26–42. http://dx.doi.org/10.21519/0234-2758-2020-36-2-26-42.

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One of the most common problems in the plant in vitro propagation is the tissue browning and subsequent necrosis, resulting from the oxidation of phenolic compounds, secondary metabolites produced in response to injury and released into the nutrient medium. This process is one of the main reasons for the decrease in the efficiency of callus formation, somatic embryogenesis, regeneration and genetic transformation of plants in vitro. Moreover, oxidative browning often leads to culture death. Therefore, the current problems in genetic and cellular engineering of a wide range of plant species can

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32

Prathanturarug, Sompop, Duangporn Angsumalee, Nuttinee Pongsiri, Suparat Suwacharangoon, and Thaya Jenjittikul. "In vitro propagation of Zingiber petiolatum (Holttum)." In Vitro Cellular & Developmental Biology - Plant 40, no. 3 (2004): 317–20. http://dx.doi.org/10.1079/ivp2003505.

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33

Bowen-O’Connor, Clare Annabel, John Hubstenberger, Cynthia Killough, Dawn Marie VanLeeuwen, and Rolston St. Hilaire. "In vitro propagation of Acer grandidentatum Nutt." In Vitro Cellular & Developmental Biology - Plant 43, no. 1 (2007): 40–50. http://dx.doi.org/10.1007/s11627-006-9005-6.

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34

Valverde-Cerdas, Lissette, Magaly Dufour, and Victor M. Villalobos. "In vitro propagation of Pithecellobium saman (Raintree)." In Vitro Cellular & Developmental Biology - Plant 33, no. 1 (1997): 38–42. http://dx.doi.org/10.1007/s11627-997-0038-2.

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35

Sanatombi, K., and G. J. Sharma. "In vitro propagation of Capsicum chinense Jacq." Biologia plantarum 52, no. 3 (2008): 517–20. http://dx.doi.org/10.1007/s10535-008-0100-x.

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36

Aparecida de Oliveira, Sandra, Maria Fátima Pires da Silva Machad, Alberto José Prioli, and Claudete Aparecida Mangolin. "In vitro propagation ofCereus peruvianus mill. (cactaceae)." In Vitro Cellular & Developmental Biology - Plant 31, no. 1 (1995): 47–50. http://dx.doi.org/10.1007/bf02632226.

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37

Llivisaca, Susana, Juan Cevallos Cevallos, Joffre Mendoza, et al. "In vitro Propagation of Mortiño (Vaccinium floribundum Kunth.)." Plant Tissue Culture and Biotechnology 30, no. 2 (2020): 167–77. http://dx.doi.org/10.3329/ptcb.v30i2.50687.

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The disinfected axillary shoots of mortino - a wild plant species were cultured in MS with different concentrations (0.002, 0.05 and 0.1%) of methyl N-(1H-benzimidazol-2-yl) carbamate to develop an in vitro protocol for micropropagation through organogenesis. They were then placed in a concentration matrix (0.1, 1.0 and 10.0 μM) whose factorial design involved BAP and IAA in WPM. Results showed a 100% disinfection at 0.05% concentrations of carbendazim and the combination of IAA and BAP at 10 μM yielded a multiplication factor of 3.35, with rooting after seven weeks. This system showed efficie

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38

Islam, M. R., Ruseli Khan, S. N. Hossain, G. Ahmed, and L. Hakim. "In vitro Clonal Propagation of Vitex negundo L.-An Important Medicinal Plant." Plant Tissue Culture and Biotechnology 19, no. 1 (2010): 113–17. http://dx.doi.org/10.3329/ptcb.v19i1.5005.

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Vitex negundo L., a perennial shrub belonging to the family Verbenaceae, is an important medicinal plant. It grows abundantly in St. Martin’s Island and commonly known as Nishinda. Generally leaf is used for medicinal purpose but root, flower and fruit also have the medicinal values (Hasan 1982). Leaf of the plant contains essential oil, an alkaloid, nishindin. Stem and bark contain flavonoid glycosides. Leaves of nishinda very effectively reduce the inflamma-tory swellings of joints in rheumatic attacks. Juice of fresh leaf removes fetid discharges and worms from ulcers. Flower oil is applied

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39

Liñán, Juana, Manuel Cantos, Javier Troncoso, José García, Antonio Fernández, and Antonio Troncoso. "Some propagation methods for cloning holm oak (Quercus ilex L.) plants." Open Life Sciences 6, no. 3 (2011): 359–64. http://dx.doi.org/10.2478/s11535-011-0007-y.

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AbstractHolm oak (Quercus ilex L.), a typical evergreen tree of the Mediterranean area, is very important due to its ecological and economical values. Propagation of this species is extremely difficult and traditionally carried out only by seed germination. In this work, mature acorns were germinated in vitro and in peat substrate in aseptic and non-aseptic conditions. Explants from the seedlings obtained were propagated in vitro in WPM plus 4 µM BA. Plant regeneration was achieved from hypocotyls and root segments cultured in vitro on modified Gamborg medium plus 20 µM BA and 20 µM NAA. 13.8%

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40

Thaniarasu, R., T. Senthil Kumar, and MV Rao. "In vitro Propagation of Plectranthus bourneae Gamble? An Endemic Red Listed Plant." Plant Tissue Culture and Biotechnology 25, no. 2 (2016): 273–84. http://dx.doi.org/10.3329/ptcb.v25i2.26260.

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An efficient protocol of in vitro propagation of Plectranthus bourneae Gamble (Lamiaceae), a valuable medicinal important and endemic Red listed plant of Western Ghats, (Tamil Nadu, India) was standardized by improved shoot multiplication from axillary bud explant. An in vitro propagation system has been reconnoitered on MS with the effective concentration BA (0.7 mg/l) followed by a combination of BA (0.7 mg/l) and TDZ (1.0 mg/l) which promoted high number of shoots. The multiple shoot rate was enhanced further by adding AdS (50 mg/l). Beneficial shoot length was achieved when cultured on MS

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41

Janarthanam, B., and E. Sumathi. "In vitro Plant Regeneration from Nodal Explants of Coleus forskohlii Briq. - An Important Medicinal Plant." Plant Tissue Culture and Biotechnology 30, no. 1 (2020): 143–48. http://dx.doi.org/10.3329/ptcb.v30i1.47799.

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An efficient in vitro mass propagation and promising protocol has been successfully standardized and developed for Coleus forskohlii through direct organogenesis from nodal explants. Nodal explants cultured onto MS basal medium supplemented with 4.44 μM BAP recorded the highest response and produced 24.3 ± 0.2 shoots per explant with an average shoot length 5.6 ± 0.4 cm after 30 days of culture. The in vitro shoots recorded higher response for development of rooting on half strength MS fortified with 2.46 μM IBA which produced the best response 7.8 ± 0.6 roots per in vitro shoot with an averag

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42

Vlasevskaya, E., and I. Mukhametshin. "Influence of the Nutrient Medium and Photoperiod on Tuberization of Potato Micro-plants of Promising Potato Varieties in vitro Culture." Bulletin of Science and Practice 5, no. 12 (2019): 177–81. http://dx.doi.org/10.33619/2414-2948/49/18.

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The results of studies on the influence of the nutrient medium and photoperiod on tuberization of micro-plants of promising potato cultivars in an in vitro culture under are presented the conditions of a potato renewal laboratory at the Udmurt Research Institute of Agriculture of the Udmurt Federal Research Center of the Ural Branch of the Russian Academy of Sciences in 2018. Two experiments were carried out to identify patterns of the influence of potato cultivation conditions in vitro on the efficiency of its propagation and to develop an improved method for propagating potato micro-tubers i

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43

Dos Reis, Michele Valquíria, Fernanda Carlota Nery, Débora De Oliveira Prudente, et al. "Propagação in vitro de Zingiber spectabile." Ornamental Horticulture 23, no. 3 (2017): 270. http://dx.doi.org/10.14295/oh.v23i3.1035.

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Zingiber spectabile is a tropical ornamental species with difficulties to obtain efficient propagation system. Thus, this study aimed to assess the in vitro propagation of Zingiber spectabile. Seed characterization was determined by measuring length, width and thickness, the weight of 1000 seeds and imbibition curve. In vitro germination of seeds was at constant (25 °C) or alternating temperatures (20-30 ºC). For optimization of in vitro multiplication, different concentrations of activated charcoal (0.0, 0.1 and 0.3%) and sucrose (0.0, 0.1, 0.3, 0.5 and 0.7 M) were evaluated. Plantlets were i

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44

Dursun, Buse, Ahu Altınkut Uncuoğlu, and Yıldız Aydin. "Chromosome stability of in vitro propagated Cucurbita cultivars." Genetics & Applications 3, no. 3 (2019): 25. http://dx.doi.org/10.31383/ga.vol3iss3pp25-32.

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Cucurbita pepo L. which is a member of Cucurbitaceae family, is a one-year plant with herbaceous stems, broad leaves and superficial scattered roots. Monoic flower structure in the Cucurbitaceae family and the differences in the maturing time of male and female organs in flowers cause an increase in the foreign fertilization rate. Therefore, there may be positive or negative changes in the existing characteristics of the species. Micro-propagation method can be performed in pumpkin species for clonal propagation, but genetic stability after tissue culture is an important consideration. Chromos

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45

Khan, Salim, Barna Goswami, Shahina Akter, et al. "In vitro mass propagation of Piper betle L." Bangladesh Journal of Botany 48, no. 3 (2019): 559–66. http://dx.doi.org/10.3329/bjb.v48i3.47917.

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An efficient in vitro regeneration system was developed for Piper betle L. through direct and indirect organogenesis from nodal segment, leaf segment and petiole explants. Highest direct regeneration was recorded when nodal explants were cultured on MS with 1.0 mg/l BAP and 1.0 mg/l Kn where 80% explants produced multiple shoots and the average number of shoots per explants were 3.20. Remarkable results on callus induction and shoot initiation were observed when the explants cultured on MS + 2.0 mg/l BAP + 0.5 mg/l Kn + 1.0 mg/l IAA. It was observed that nodal explants were showed best respons

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Sbay, H., J. Guillot, P. Danthu, and D. Prat. "In vitro propagation of interspecific hybrids in Alnus." Annales des Sciences Forestières 46, Supplement (1989): 155s—157s. http://dx.doi.org/10.1051/forest:19890535.

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MICLEA, Ileana, and Raluca CHIFOR. "Germination, in vitro Propagation and Acclimatization in Lavandula Angustifolia." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies 75, no. 2 (2018): 105. http://dx.doi.org/10.15835/buasvmcn-asb:2018.0017.

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This research focused on finding the best method for seed in vitro germination in Lavandula angustifolia and optimizing the medium for plant propagation. Seeds were sterilized and subjected to various treatments to break dormancy, then placed on half-strength MS (1/2MS) or distilled H2O + phytagel. Germination percentages were assessed and plantlets propagated on MS without growth regulators or with zeatin (0.5, 1, 2 mg/l), 1 mg/l BA + 0.5 mg/l IBA, 2 mg/l BA + 1 mg/l IBA or 3 mg/l BA + 1.5 mg/l IBA. After 8 weeks growth parameters were recorded and plants were acclimatized. Immersion in 20 mg

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Devi, C. Subathra, and V. Mohana Srinivasan. "In vitro Propagation of Gymnema sylvestre." Asian Journal of Plant Sciences 7, no. 7 (2008): 660–65. http://dx.doi.org/10.3923/ajps.2008.660.665.

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Rajabudeen, E., A. Saravana Ganthi, S. Sivasubramanian, and M. Padma Sorna Subramanian. "In vitro regeneration of Indigofera viscosa lam." Journal of Bio-Science 22 (October 21, 2016): 53–58. http://dx.doi.org/10.3329/jbs.v22i0.30009.

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Context: In vitro propagation or tissue culture of plants offers a rapid means of producing large quality of clonal planting stock and propagation that are difficult to establish conventionally. Biotechnological tools are important for multiplication and genetic enhancement of the plants by adopting techniques such as in vitro regeneration and genetic transformations.Objective: Effect of different plant growth regulators (PGRs) and their concentration on multiple shoot regeneration and callus formation was studied in Indigofera viscosa.Materials and Methods: In vitro plant regeneration was ach

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Yu, Rongpei, Ying Cheng, Yanfei Pu, Fan Li, and Shugang Lu. "In Vitro Propagation of Resurrection Plant Selaginella pulvinata Using Frond Tips as Explants." HortScience 56, no. 3 (2021): 313–17. http://dx.doi.org/10.21273/hortsci15546-20.

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The resurrection plant Selaginella pulvinata (Hook. & Grev.) Maxim is used as an ornamental and medicinal plant. It is also a good candidate for exploring the desiccation tolerance of resurrection plants. However, there is not an efficient propagation method for S. pulvinata. In the present study, we evaluated the establishment of in vitro propagation of S. pulvinata using frond tips as explants. The original shoot induction, adventitious shoot proliferation and plantlet growth media, and substrate type of plantlet acclimatization were investigated. The highest induction rate of original s

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