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1

Ramarumo, Luambo Jeffrey, Alfred Maroyi, and Milingoni Peter Tshisikhawe. "Plant species used for birdlime-making in South Africa." Bangladesh Journal of Botany 49, no. 1 (March 31, 2020): 117–24. http://dx.doi.org/10.3329/bjb.v49i1.49104.

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Plants used for making birdlime and indigenous knowledge associated with the practice in Soutpansberg region, Vhembe Biosphere Reserve, Limpopo province, South Africa have been documented. Twelve birdlime-making plant species belonging to Apocynaceae, Celastraceae, Euphorbiaceae, Loranthaceae, Moraceae and Sapotaceae families were recorded. The common species included Maytenus peduncularis (Sond.) Loyes cited by 23.6% informants, Euphorbia pulvinata Marloth (17.2%) and Landolphia kirkii Dyer (12%). Plant parts used were latex (50%), fruit (34%), root (8) and the mixture of latex and fruit (8%). Documentation of plant species used for birdlime-making is of great interest, not only for preservation of the Vhavenḓa's traditional culture, but also for promoting economic subsistence, nutritional value and livelihood amongst poor and marginalized people.
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BOROVEC, ROMAN, and JIŘÍ SKUHROVEC. "A taxonomic study of the South African terricolous weevil genus Pentatrachyphloeus Voss (Coleoptera: Curculionidae: Entiminae: Trachyphloeini)." Zootaxa 4574, no. 1 (March 29, 2019): 1. http://dx.doi.org/10.11646/zootaxa.4574.1.1.

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The genus Pentatrachyphloeus Voss, 1974, with two known species, is redefined and compared with related genera. An additional thirty seven new species are described here: P. andersoni sp. nov. (South Africa, Mpumalanga); P. baumi sp. nov. (South Africa, Gauteng); P. brevithorax sp. nov. (South Africa, KwaZulu-Natal); P. bufo sp. nov. (South Africa, Mpumalanga); P. endroedyi sp. nov. (South Africa, Mpumalanga); P. exiguus sp. nov. (South Africa, Mpumalanga); P. frici sp. nov. (South Africa, Limpopo); P. grobbelaarae sp. nov. (South Africa, KwaZulu-Natal); P. hanzelkai sp. nov. (South Africa, KwaZulu-Natal); P. holubi sp. nov. (South Africa, Mpumalanga); P. howdenae sp. nov. (South Africa, Mpumalanga); P. hystrix sp. nov. (South Africa, Mpumalanga); P. insignicornis sp. nov. (South Africa, KwaZulu-Natal); P. kalalovae sp. nov. (South Africa, Gauteng); P. kuscheli sp. nov. (South Africa, KwaZulu-Natal); P. laevis sp. nov. (South Africa, Mpumalanga); P. lajumensis sp. nov. (South Africa, Limpopo); P. leleupi sp. nov. (Zimbabwe, Manica); P. lesothoensis sp. nov. (Lesotho, Qacha’s Nek); P. machulkai sp. nov. (South Africa, Free State); P. marshalli sp. nov. (South Africa, KwaZulu-Natal); P. muellerae sp. nov. (South Africa, Mpumalanga); P. musili sp. nov. (South Africa, Limpopo); P. ntinini sp. nov. (South Africa, KwaZulu-Natal); P. oberprieleri sp. nov. (South Africa, Gauteng, North West); P. pavlicai sp. nov. (South Africa, Free State); P. rudyardi sp. nov. (South Africa, Limpopo); P. schoemani sp. nov. (South Africa, Limpopo); P. soutpansbergensis sp. nov. (South Africa, Limpopo); P. spinimanus sp. nov. (South Africa, Mpumalanga); P. stingli sp. nov. (South Africa, Limpopo); P. tenuicollis sp. nov. (South Africa, Mpumalanga); P. tuberculatus sp. nov. (South Africa, Mpumalanga); P. vavrai sp. nov. (South Africa, Eastern Cape); P. vossi sp. nov. (South Africa, Mpumalanga); P. vrazi sp. nov. (South Africa, Limpopo) and P. zikmundi sp. nov. (South Africa, Free State). All of the species are keyed and illustrated; ecological information is presented only where available. All species seem to be very localised, being known only from one or only a very limited number of localities. Immature stages or host plants are not known for any of the species. The species are distributed as follows: South Africa: Mpumalanga (13), Limpopo (8), KwaZulu-Natal (7), Free State (3), Gauteng (3), Eastern Cape (3), North West (1); Lesotho: Qacha’s Nek (1) and Zimbabwe: Manica (1).
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3

Rasethe, Marula Triumph, Sebua Silas Semenya, and Alfred Maroyi. "Medicinal Plants Traded in Informal Herbal Medicine Markets of the Limpopo Province, South Africa." Evidence-Based Complementary and Alternative Medicine 2019 (April 16, 2019): 1–11. http://dx.doi.org/10.1155/2019/2609532.

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Trading of herbal medicines generates economic opportunities for vulnerable groups living in periurban, rural, and marginalized areas. This study was aimed at identifying medicinal plant species traded in the Limpopo province in South Africa, including traded plant parts, conservation statutes of the species, and harvesting methods used to collect the species. Semistructured questionnaire supplemented by field observation was used to collect data from owners of 35 informal herbal medicine markets in the Limpopo province. A total of 150 medicinal plant products representing at least 79 plant species belonging to 45 botanical families, mainly the Fabaceae (11.4%), Asteraceae (7.6%), and Hyacinthaceae (6.3%), were traded in the study area. Roots (50.0%), bulbs (19.0%), and bark (16.0%) were the most frequently sold plant parts. Some of the traded species which includeAlepidea amatymbica, Bowiea volubilis, Brackenridgea zanguebarica, Clivia caulescens,Dioscorea sylvatica,Elaeodendron transvaalense, Encephalartos woodii,Eucomis pallidiflorasubsp.pole-evansii,Merwilla plumbea,Mondia whitei,Prunus africana, Siphonochilus aethiopicus, Synaptolepis oliveriana,andWarburgia salutarisare of conservation concern and listed on the South African Red Data List.Findings of this study call for effective law enforcement to curb illegal removal of wild plants especially those species that are at the verge of extinction.
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4

BIDZILYA, OLEKSIY. "New host-plants records of Afrotropical Gelechiidae (Lepidoptera), with description of three new species." Zootaxa 4952, no. 3 (April 12, 2021): 495–522. http://dx.doi.org/10.11646/zootaxa.4952.3.4.

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First and additional host-plant records for 38 species of Gelechiidae from South Africa and Kenya are presented. An annotated list with brief descriptions of larval biology and known geographical distributions is presented. Three species are described as new: Mesophleps kruegeri sp. nov. (Namibia: Kavango Region and South Africa: Limpopo Province), Istrianis inquilinus sp. nov. (South Africa: North West Province), and Teleiopsis sharporum sp. nov. (South Africa: Limpopo Province). The new combination Istrianis epacria (Bradley, 1965) comb. nov. is proposed. The male genitalia of Hypatima melanecta (Meyrick, 1914) is described for the first time. It is noticed that male hitherto associated with T. commaculata (Meyrick, 1918) in fact refers to T. pundamilia Bidzilya & Mey, 2018. Hypatima stasimodes (Meyrick, 1931), Dichomeris coenulenta (Meyrick, 1927), Dichomeris eustacta Meyrick, 1921, Neotelphusa similella Janse, 1958 and Argophara epaxia Janse, 1963 are recorded for the first time from South Africa, and Istrianis epacria is new for Kenya. The adults, male and female genitalia are illustrated for some species mentioned in paper. The first record of the genus Teleiopsis Sattler, 1960 from South Africa is briefly discussed.
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SEMENYA, SEBUA SILAS, and ALFRED MAROYI. "Source, harvesting, conservation status, threats and management of indigenous plant used for respiratory infections and related symptoms in the Limpopo Province, South Africa." Biodiversitas Journal of Biological Diversity 20, no. 3 (March 3, 2019): 789–810. http://dx.doi.org/10.13057/biodiv/d200325.

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Abstract. Semenya SS, Maroyi A. 2019. Source, harvesting, conservation status, threats and management of indigenous plant used for respiratory infections and related symptoms in the Limpopo Province, South Africa. Biodiversitas 20: 789-810. This survey explored Bapedi traditional healer’s (THs) practices pertinent to native plants used to treat respiratory infections (RIs) and related symptoms (RSs). Semi-structured questionnaires and participatory observations were used to gather information from 240 THs in the Limpopo Province, South Africa. 186 plants from 75 families were harvested by these THs, mainly from the communal lands (81.2%), throughout the year. Plant parts used for RIs and RSs remedies was destructively harvested in wilderness compared to homegardens. Most (n=174) species from which these parts are obtained appears on the South African National Red Data List of plants, with 88.5% having a list concern status. This included Adansonia digitata, Boscia albitrunca, Catha edulis, Securidaca longepedunculata and Sclerocarya birrea which are also protected under the National Forest Act of 1998 (Act no. 84 of 1998). A further, 8.6% (n=15) of Red Data Listed plants are of conservation concern, with various status namely near threatened (38.3%), declining (20%), data deficient (13.3%), critically endangered and vulnerable (3.3%, for each), as well as endangered (6.6%). There were both consensus and disjunction amongst THs and Red Data List regarding the status of plants in the wild. This study provides valuable data for the conservation of medicinal plants in Limpopo Province.
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Hahn, N., and G. J. Bredenkamp. "LAMIACEAE." Bothalia 37, no. 1 (August 18, 2007): 37–40. http://dx.doi.org/10.4102/abc.v37i1.298.

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7

Hurter, P. J. H., and A. E. Hurter. "FABACEAE." Bothalia 34, no. 2 (September 3, 2004): 109–12. http://dx.doi.org/10.4102/abc.v34i2.422.

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8

BOTHA, J., E. T. F. WITKOWSKI, and C. M. SHACKLETON. "Market profiles and trade in medicinal plants in the Lowveld, South Africa." Environmental Conservation 31, no. 1 (March 2004): 38–46. http://dx.doi.org/10.1017/s0376892904001067.

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Rising demand for medicinal plants has led to increased pressure on wild plant populations. This, combined with shrinking habitats, means that many species in South Africa are now facing local extinction. In 1997, a study was initiated to determine the extent of trade in medicinal plants in the South African Lowveld (the low lying plains to the east of the Drakensberg escarpment), and to investigate socio-economic factors influencing trade and resource management. Trade was not as extensive in the Lowveld as in major urban markets such as Durban or the Witwatersrand (Johannesburg and surrounding towns), either in terms of the quantity, number or range of species sold, or the numbers of people relying on the trade for an income. In markets assessed in Mpumalanga Province, 176 species were identified (71% of the vernacular names encountered in the market place), representing 69 plant families. In Limpopo, 70 different species were identified (84% of the vernacular names encountered in the market place), representing 40 families. Imports were significant in Mpumalanga (33% of the plants on offer), mainly from Mozambique. A detrended correspondence analysis showed substantial differences between species traded in Mpumalanga and those sold in Limpopo. There was little variation in the species stocked by vendors in Mpumalanga, regardless of the season, the attributes of the seller, or whether business was carried out in urban or rural areas. In contrast, there was considerable variation in the stock inventories of the Limpopo traders. Despite the lower levels of local trade, increased harvesting pressure is being experienced regionally, to meet demand in metropolitan centres such as the Witwatersrand. This study showed considerable local variation and complexities in the harvesting and marketing of medicinal plants, with both a national and an international dimension. This dual spatial scale presents both opportunities and challenges in the management of these plants, which need to be addressed simultaneously, particularly with respect to research requirements and development of predictive models and capacity. Cooperation in conservation strategies and policies is required at regional, national and international levels, while ensuring that management initiatives take into account local market conditions and the socio-economic realities facing both consumers and those who depend on the trade for their livelihoods.
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9

Klopper, R. R., and G. F. Smith. "Asphodelaceae: Alooideae - Aloe Hahnii, a new species in the section Pictae, in the Soutpansberg centre of endemism, Limpopo Province, South Africa." Bothalia 39, no. 1 (August 11, 2009): 98–100. http://dx.doi.org/10.4102/abc.v39i1.233.

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10

Lemmer, P. "Jamesbrittenia bergae (Scrophulariaceae), a distinctive new species from Limpopo, South Africa." Bothalia 33, no. 2 (September 9, 2003): 141–43. http://dx.doi.org/10.4102/abc.v33i2.441.

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Jamesbrittenia bergae P.Lemmer is a new species from the Farm Brakvallei near Thabazimbi in Limpopo [Northern Province]. Large, medium-textured. bright scarlet flowers w ith yellow throats are borne singly in upper leaf axils; the flowering branches, although terminal, do not form elongated racemes: leaves are greyish green, coarsely serrated. It grows in full sun on ferricrete outcrops.
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11

Keet, Jan-Hendrik, and David M. Richardson. "A rapid survey of naturalized and invasive eucalypt species in southwestern Limpopo, South Africa." South African Journal of Botany 144 (January 2022): 339–46. http://dx.doi.org/10.1016/j.sajb.2021.09.008.

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12

Silas Semenya, Sebua, and Alfred Maroyi. "Assessment of Useful Alien Plant Species Cultivated and Managed in Rural Home Gardens of Limpopo Province, South Africa." Scientifica 2020 (April 28, 2020): 1–11. http://dx.doi.org/10.1155/2020/3561306.

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Several communities in developing countries derive substantial part of their livelihood needs from alien plants cultivated and managed in home gardens. The aim of this study was to assess useful alien plant species cultivated and managed in home gardens of Limpopo province in South Africa. Semistructured interviews, personal observation, and guided walks with 300 participants between January 2015 and December 2016 were employed to obtain data on names of alien plants cultivated in home gardens and their use categories. A total of 101 plant species belonging to 44 families were recorded from the study area. More than half of the species (66.3%) belonged to 14 families, Fabaceae, Asteraceae, Rosaceae, Solanaceae, Lamiaceae, Anacardiaceae, Poaceae, Amaranthaceae, Apocynaceae, Brassicaceae, Cactaceae, Euphorbiaceae, Moraceae, and Myrtaceae. Twenty-six use categories of alien plants were identified in this study with the majority of species (75.2%) used for medicinal purposes, followed by ornamental (33.7%), edible fruits (24.8%), spices (16.8%), vegetables (16.8%), shade (11.9%), beverages (10.9%), construction materials (8.9%), firewood (7.9%), and hedge (7.9%). These findings corroborate the existing body of knowledge emphasizing the importance of plants grown and managed in home gardens to the livelihood needs of local communities.
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SMITH, GIDEON F. "Aloe ×retiefii (Asphodelaceae subfam. Alooideae), a new nothospecies from northeastern South Africa, with A. globuligemma and A. marlothii as parents." Phytotaxa 468, no. 1 (October 23, 2020): 150–54. http://dx.doi.org/10.11646/phytotaxa.468.1.12.

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Aloe marlothii Berger (1905: 87) (Asphodelaceae subfam. Alooideae) (Fig. 1), a single-stemmed, tree-like aloe, occurs widespread in northeastern South Africa and adjacent parts of Botswana, for example near Lobatse in the southeast of the country from where it was originally described (Fig. 2), Zimbabwe, and Mozambique (see maps in Klopper & Smith 2010: 92, Fig. 32 and Van Wyk & Smith 2014: 68). Wherever A. marlothii co-occurs with other species of Aloe Linnaeus (1753: 319), hybrids are produced (Reynolds 1950, Smith & Figueiredo 2015). Aloe globuligemma Pole-Evans (1915: 30, Plates X [Figs 1 and 2] and XI [Figs 1, 2, and 3]) (Fig. 3), a stemless, medium-sized aloe, is restricted to the more subtropical parts of northeastern South Africa, southern Zimbabwe, and Mozambique (see map in Van Wyk & Smith 2014: 158). Where these two species, A. globuligemma and A. marlothii, co-occur, for example at Lebowakgomo, about 50 km southeast of Polokwane, the capital of South Africa’s Limpopo province, a bewildering range of striking hybrids with considerable horticultural potential are found (Reynolds 1950: 446, 484, Retief 2018). This hybrid also occurs a bit further south of the Lebowakgomo location, as well as elsewhere in the Limpopo province, in Sekhukhuneland in the vicinity of Steelpoort and Ohrigstad.
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Semenya, Sebua Silas, and Alfred Maroyi. "Plants Used by Bapedi Traditional Healers to Treat Asthma and Related Symptoms in Limpopo Province, South Africa." Evidence-Based Complementary and Alternative Medicine 2018 (July 19, 2018): 1–33. http://dx.doi.org/10.1155/2018/2183705.

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To the best of our knowledge there are presently no ethnobotanical surveys focusing on the utilisation of herbal remedies for asthma in South Africa. The present study is therefore an attempt to fill this gap in knowledge. A total of 140 Bapedi traditional healers (THs) practicing in the Capricorn, Sekhukhune, and Waterberg districts of the Limpopo Province (South Africa) were queried using semistructured questionnaires, supplemented by field observations during face-to-face interview. A total of 104 medicinal plant species (92 indigenous and 12 exotics) belonging to 92 genera, distributed across 54 botanical families, mostly the Asteraceae and Fabaceae (18.5%, for each) as well as Malvaceae (12.9%), were used as antiasthmatics and related symptoms by these THs. Most of the plants were trees and herbs (37.5%, for each), with root (57%), leaf (15.8%), and bark (7.5%), respectively, being the saliently used parts for preparation of remedies.Clerodendrum ternatum,Cryptocarya transvaalensis,Lasiosiphon caffer,Enicostema axillare,Mimusops obovata,Sclerocarya birrea, andStylochaeton natalensiswere widely used and valued by all THs across the surveyed districts. Furthermore, these taxa also scored both the highest use value and fidelity level indexes as asthma therapies. Overall, the larger number of species documented in the present study is recorded for the first time in literature as asthma and/or related symptoms remedies. Our study finding generally contributes towards an establishment of South African database of herbal therapies used traditionally against these conditions.
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Mbedzi, M., M. P. Tshisikhawe, N. I. Sinthumule, and S. Rahlao. "The rate of regeneration of native plant species after the eradication of invasive alien plant species in Limpopo Province, South Africa." South African Journal of Botany 115 (March 2018): 298. http://dx.doi.org/10.1016/j.sajb.2018.02.081.

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Kurugundla, C. N., B. Mathangwane, S. Sakuringwa, and G. Katorah. "Alien Invasive Aquatic Plant Species in Botswana: Historical Perspective and Management." Open Plant Science Journal 9, no. 1 (June 14, 2016): 1–40. http://dx.doi.org/10.2174/1874294701609010001.

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Aquatic ecosystems in Botswana have been under threat by the aquatic alien invasive plant species viz., salvinia Salvinia molesta Mitchell, water lettuce Pistia stratiotes L., and water hyacinth Eichhornia crassipes (Mart.) Solms-Laub. While salvinia has been termed the major threat to the Botswana wetlands, water lettuce and water hyacinth are considered to be of minor importance. This review presents the species biology, distribution, historical spread, negative impacts, control achieved right from their discovery in the country by referring to their control and management in the world. Having infested the Kwando-Linyanti-Chobe Rivers in the 1970s, salvinia was initially tried by the use of herbicides, paraquat and glyphosate, between 1972 and 1976. With the discovery of the host specific biological control weevil Cyrtobagous salviniae Calder and Sands in 1981, the weevil was introduced by Namibians on Kwando and Chobe Rivers in 1983 and by Botswana in 1986 in the Okavango Delta. While the control was slowly establishing in Kwando-Linyanti-Chobe Rivers, it became apparent that lakes and perennial swamps within and outside Moremi Game Reserve of the Okavango Delta were infested with salvinia from 1992 onwards. With continuous and sustained liberation of the weevil in the Kwando-Linyanti-Chobe Rivers and in the Okavango Delta between 1999 and 2000, salvinia control was achieved by 2003, and since then the weevil constantly keeps the weed at low levels. The success is mainly due to sustainable monitoring through the application of physical and biological control methods. However, salvinia is still threatening the Okavango Delta due to factors such as tourism activities, boat navigation fishing and transporttion by wild animals. The first occurrence of water lettuce was recorded on Kwando and Chobe Rivers in 1986. Its biocontrol weevil Neohydronomous affinis Hustache was released in the year 1987. The weevil became extinct in Selinda Canal and Zibadianja Lake on Kwando River due to dry and wet events for over 10 years and the weed had been under control biologically on Chobe River. Having surface covered the Selinda and a part of the Zibadianja in high flood and rainfall in 1999/2000 season, research was undertaken to contain water lettuce, which led to its eradication by 2005. Regular physical removal of the water lettuce prior to fruit maturity is an effective method of control or eradicating the weed in seasonal water bodies. The Limpopo Basin (shared by Botswana, South Africa, Zimbabwe and Mozambique) has become vulnerable to water hyacinth infestation. Water hyacinth infested the trans-boundary Limpopo River in 2010 sourced from Hartbeesport Dam on Crocodile River in South Africa. Botswana and South Africa have been consulting each other to implement integrated control of the weed jointly in the Limpopo River. Water hyacinth could be a continuous threat to the dams and the rivers in the Limpopo basin if its control is not taken seriously. These three species are found growing in Botswana in a range of pH between 4.5 and 10.3 and in the range of conductivities between 20 and 580 µS cm-1. Range of soluble nitrates, phosphates and potassium in the habitats of salvinia infestations were 0.02 to 1.5, 0.01 to 1.78 and 0.3 to 6.92 mg L-1 respectively. Water lettuce infestation in the seasonal Selinda Canal had a maximum of 4.7 mg L-1 nitrates, 2.8 mg L-1 phosphates and 7.9 mg L-1 potassium. Nevertheless, these three nutrients were in the range of 0.41 to 9.56 mg L-1, 0.2 to 2.9 mg L-1, and 7.7 to 11.53 mg L-1 respectively in the Limpopo River where water hyacinth infestations were observed. These nutrients were considerably high during decomposition phase of biological control of weeds. The Government of Botswana “regulates the movement and importation of boats and aquatic apparatus, to prevent the importation and spread of aquatic weeds both within and from the neighboring countries” by “Aquatic Weed (Control) Act” implemented in 1986. These measures, combined with communities, conservation groups, NGOs and public awareness campaigns, have highlighted the gravity of aquatic weeds spreading into wetlands, dams and other water bodies. In conclusion, the Government of Botswana is committed and supportive through the Department of Water Affairs in protecting the wetlands of the country efficiently and prudently.
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Mashile, S. P., M. P. Tshisikhawe, and M. H. Ligavha-Mbelengwa. "Tree layer species composition of the Nylsvley Nature Reserve woodland community in Limpopo Province, South Africa." South African Journal of Botany 76, no. 2 (April 2010): 413. http://dx.doi.org/10.1016/j.sajb.2010.02.074.

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Masoko, Peter, and Kulani Mashudu Nxumalo. "Validation of Antimycobacterial Plants Used by Traditional Healers in Three Districts of the Limpopo Province (South Africa)." Evidence-Based Complementary and Alternative Medicine 2013 (2013): 1–7. http://dx.doi.org/10.1155/2013/586247.

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The aim of the study was to scientifically evaluate the antimycobacterial activity of selected indigenous medicinal plants from the Limpopo Province used for the treatment of humans with symptoms ofMycobacterium tuberculosis. The leaves of five plant species (Apodytes dimidiata, Artemisia, Combretum hereroense, Lippia javanica, and Zanthoxylum capense) were collected from the Lowveld National Botanical Garden in Nelspruit, South Africa. The dried leaves were powdered and extracted using hexane, dichloromethane, acetone, and methanol. Antimycobacterial activity was evaluated using microdilution assay and bioautography andρ-iodonitrotetrazolium violet (INT) as indicator. Antioxidant activities were determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH). Phytochemical content of extracts was further evaluated. The acetone extracts ofL. javanicadisplayed antioxidant activity on BEA chromatogram. T Acetone extracts ofA. afrahad MIC value of 0.39 mg/mL againstMycobacterium smegmatisATCC 1441. Acetone extracts ofC. hereroenseandL. javanicahad MIC value of 0.47 mg/mL. Four bands that inhibited the growth ofM. smegmatiswere observed atRfvalues of 0.12, 0.63, and 0.87 on BEA and 0.73 on EMW. The plant speciesA. dimidiata, A. afra, C. hereroense,andL. javanicain this study demonstrated their potential as sources of anti-TB drug leads.
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Maema, Lesibana Peter, Martin Potgieter, and Salome Mamokone Mahlo. "INVASIVE ALIEN PLANT SPECIES USED FOR THE TREATMENT OF VARIOUS DISEASES IN LIMPOPO PROVINCE, SOUTH AFRICA." Africa Journal of Traditional Complementary and Alternative Medicine 13, no. 4 (July 3, 2016): 223–31. http://dx.doi.org/10.21010/ajtcam.v13i4.29.

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20

Rampedi, Isaac T., and Jana Olivier. "Traditional Beverages Derived from Wild Food Plant Species in the Vhembe District, Limpopo Province in South Africa." Ecology of Food and Nutrition 52, no. 3 (May 2013): 203–22. http://dx.doi.org/10.1080/03670244.2012.706131.

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Gololo, S. S., N. S. Mapfumari, and M. A. Mogale. "COMPARATIVE QUANTITATIVE PHYTOCHEMICAL ANALYSIS OF THE LEAVES OF SENNA ITALICA COLLECTED FROM DIFFERENT AREAS IN LIMPOPO PROVINCE, SOUTH AFRICA." International Journal of Pharmacy and Pharmaceutical Sciences 10, no. 2 (February 1, 2018): 67. http://dx.doi.org/10.22159/ijpps.2018v10i2.22950.

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Objective: The current study was aimed at comparative quantitative phytochemical analysis of the leaves of S. italica collected from four districts in Limpopo province, South Africa, in order to establish whether geographical location have an effect on the accumulation of phytochemicals within the leaves of the plant species under study.Methods: The leaves of S. italica were collected from four districts in Limpopo province of South Africa namely; Capricorn, Sekhukhune, Vhembe and Waterberg districts, dried, ground to powder and extracted using different organic solvents. The extracts of the leaf samples from different locations were subjected to quantitative phytochemical analysis for total phenolic content, total tannin content, total flavonoid content and total saponin content using spectrophotometric measurements. The resultant quantities were analysed for statistical differences.Results: The leaf samples of S. italica from the four districts in Limpopo province showed significant differences (*p<0.05) in their phytochemical quantities, with main data expressed as mean±SD. Total phenolic content was in highest amounts in leaf samples from Waterberg district compared to samples from other districts. Total tannin content was in highest amounts in the Vhembe district leaf samples compared to samples from other districts. Total flavonoid content was in highest amounts in the leaf samples from Waterberg district compared to samples from other districts. Total saponin content was in highest amounts in the Vhembe district leaf samples compared to samples from other districts.Conclusion: The findings of the study thus suggest that geographical location has an effect on the accumulation of phytochemicals in the leaves of S. italica.
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Mbedzi, M., M. H. Ligavha-Mbelengwa, and M. P. Tshisikhawe. "The association and interaction of Acacia decurrens Willd. with itsindigenous species at the Waterberg area, Limpopo Province, South Africa." South African Journal of Botany 103 (March 2016): 331. http://dx.doi.org/10.1016/j.sajb.2016.02.103.

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Ndhlovu, P. T., O. Mooki, W. Otang Mbeng, and A. O. Aremu. "Plant species used for cosmetic and cosmeceutical purposes by the Vhavenda women in Vhembe District Municipality, Limpopo, South Africa." South African Journal of Botany 122 (May 2019): 422–31. http://dx.doi.org/10.1016/j.sajb.2019.03.036.

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24

Nenzhelele, E., and M. P. Tshisikhawe. "The diversity and the spread of alien invasive plant species in the Nylsvley Nature Reserve, Limpopo Province, South Africa." South African Journal of Botany 98 (May 2015): 213–14. http://dx.doi.org/10.1016/j.sajb.2015.03.167.

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Akanwe Asiwe, Joseph Nwafor. "Yield and Economic Assessments of Five Cowpea Varieties in Cowpea-Maize Strip Intercropping in Limpopo Province, South Africa." International Journal of Agriculture and Biology 25, no. 01 (January 1, 2021): 27–32. http://dx.doi.org/10.17957/ijab/15.1634.

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Farmers‟ traditional cropping practice in Limpopo Province is to mix and broadcast crops at planting without definite row arrangement. Although this practice is very easy and cost-saving, it leads to low plant density, hinders farm input application, and results in low crop yields and poor return on investment. Strip intercropping, where crops are planted with definite row arrangement, reduces inter-species competition, optimises plant population, and increases crop yield. Five cowpea varieties i.e. „TVu 13464‟, „IT86D-1010‟, „Glenda‟, „IT82E-16‟ and „IT87K-499-35‟ and maize was grown under strip intercropping, monocropping, and mixed intercropping as a control during two seasons. During both years, significant interactions were obtained between the cowpea varieties and the cropping systems in most of the variables measured. Cowpea sown in the strip intercropping performed better compared those sown in mixed intercropping. Cowpea varieties „IT86D-1010‟, „IT82E-16‟ and „IT87K-499-35‟ harvested more grain yield under monocropping and strip intercropping than under mixed intercropping. The land equivalent ratio (LER) of strip intercropping during the two seasons ranged between 1.25 and 2.29 and was higher compared to mixed intercropping, which ranged between 0.50 and 1.32. In conclusion, cowpea varieties „IT86D-1010‟, „IT82E-16‟ and „IT87K-499-35‟ sown with maize as strip intercropping resulted more profits under intercropping systems and were recommended for cultivation by farmers in the Limpopo region with low rainfall. Moreover, grain yield, LER, and net profit achieved by strip intercropping was three-fold more than mixed intercropping. © 2021 Friends Science Publishers
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26

Ramalevha, T. L., M. H. Ligavha-Mbelengwa, and M. P. Tshisikhawe. "The impact of Chromolaena odorata and Lantana camara on native species diversity in parts of Vhembe District Municipality, Limpopo Province, South Africa." South African Journal of Botany 115 (March 2018): 327. http://dx.doi.org/10.1016/j.sajb.2018.02.186.

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Gololo, Sechene S., Chepape J. Semenya, Mutendela T. Olivier, Lesibana J. Sethoga, Emelinah H. Mathe, and Rejoice B. Maseko. "Metabolite Profiling of Different Tissues of Barleria dinteri through the GC-MS Analysis." Asian Journal of Chemistry 33, no. 6 (2021): 1336–40. http://dx.doi.org/10.14233/ajchem.2021.23192.

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Barleria dinteri is a medicinal plant with distribution in the Limpopo, Gauteng and Mpumalanga provinces of South Africa as well as in Botswana, Swaziland and Namibia with exclusive specific habitation on limestone-rich soil. The plant species is used by traditional healers for wound healing and treatment of some intestinal tumours, as well as to relieve joint pains and toothache. The present study was aimed at the metabolite profiling of the different tissues (branches, flowers, leaves, roots) of Barleria dinteri using GC-MS analysis. Different extracts of the plant parts samples were subjected to GC-MS analysis and detected compounds were compared for presence amongst the different tissues. The results of the study revealed that all different parts (branches, flowers, leaves and roots) of B. dinteri, possess compounds that are detectable through GC-MS analysis with most compounds detected in the aerial parts, particularly the flowers. The results of the current study could serve as a basis for the possible plant parts substitution of the roots of B. dinteri with the aerial parts and the exploration of the pharmacological properties of the flowers for sustainable uses of the plant species for medicinal purposes.
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MP, Tshisikhawe, MW van Rooyen, and RB Bhat. "An evaluation of the extent and threat of bark harvesting of medicinal plant species in the Venda Region, Limpopo Province, South Africa." Phyton 81, no. 1 (2012): 89–100. http://dx.doi.org/10.32604/phyton.2012.81.089.

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STONE, ROBERT DOUGLAS, IMERCIA GRACIOUS MONA, DAVID STYLES, JOHN BURROWS, and SYD RAMDHANI. "Taxonomic revision of South African Memecylon (Melastomataceae—Olisbeoideae), including three new species." Phytotaxa 418, no. 3 (September 25, 2019): 237–57. http://dx.doi.org/10.11646/phytotaxa.418.3.1.

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Earlier works recognised two South African species Memecylon bachmannii and M. natalense within M. sect. Buxifolia, but recent molecular analyses have revealed that M. natalense as previously circumscribed is not monophyletic and includes several geographically outlying populations warranting treatment as distinct taxa. In this revision we recognise five endemic South African species of which M. bachmannii and M. natalense are both maintained but with narrower circumscriptions, and M. kosiense, M. soutpansbergense and M. australissimum are newly described. Memecylon kosiense is localised in north-eastern KwaZulu-Natal (Maputaland) and is closely related to M. incisilobum of southern Mozambique. Memecylon soutpansbergense, from Limpopo Province, was previously confused with M. natalense but is clearly distinguished on vegetative characters. Memecylon australissimum occurs in the Eastern Cape (Hluleka and Dwesa-Cwebe nature reserves) and has relatively small leaves like those of M. natalense, but the floral bracteoles are persistent and the fruit is ovoid as in M. bachmannii. We further designate a lectotype for M. natalense, provide an updated key to the South African species of Memecylon, and provisionally assess the status of each species in accordance with International Union for Conservation of Nature criteria.
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Vuma, X. H., and M. P. Tshisikhawe. "An assessment of the ecological impact of Solanum mauritianum Blanco on species diversity of shrub and tree layer in Vleifontein, Limpopo Province, South Africa." South African Journal of Botany 109 (March 2017): 374. http://dx.doi.org/10.1016/j.sajb.2017.01.194.

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Shikwambana, Ndzalama, and Salome Mamokone Mahlo. "A Survey of Antifungal Activity of Selected South African Plant Species Used for the Treatment of Skin Infections." Natural Product Communications 15, no. 5 (May 2020): 1934578X2092318. http://dx.doi.org/10.1177/1934578x20923181.

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Dermatophytosis is a fungal infection of the skin caused by a group of pathogenic fungi known as dermatophytes. Therefore, we investigated those medicinal plants that were being used by local people and traditional healers to treat skin infections in the Mopani District, Limpopo Province. A survey was conducted through the use of a semistructured program to gather information on the common names, plant parts used, methods of preparation, and administration of the medicine. Findings of the survey revealed that over 30 plant species were used for the treatment of skin infections in the area. The most common local mode of medicinal preparation to treat skin was decoction (37%), followed by paste (21%), infusion (19%), poultice (9%), smoke (7%), maceration (5%), and steam (2%). Of the species, only 12 ( Ficus sur L., Peltophorum africanum Sond., Vangueria infausta Burch. subsp. infausta, Diospyros mespilliformis Hochst. ex. A. DC., Ziziphus mucronata Willd. subsp. mucronata, Euclea divinorum Hiern, Ximenia caffra Sond., Dombeya rotundifolia Hochst., Ficus sycomorus L., Sideroxylon inerme L. subsp inerme, Parinari curattellifolia Planch. ex Benth., and Maytenus undata (Thunb.) Blakelock) were selected based on literature and ethnobotanical information. We further investigated the antifungal activity of acetone and aqueous extracts of the above mentioned selected plant species using serial dilution assay against Trichophyton rubrum, Microsporum canis, and Candida albicans. All plant extracts were active against the tested microorganisms with minimum inhibitory concentration (MIC) values ranging between 0.02 mg/mL and 1.25 mg/mL. In the bioautography assay, more active compounds were visible in acetone and water extracts of E. divinorum. No active compounds were observed in some plant extracts with excellent antifungal activity, as shown in the microdilution assay. Findings, in general, suggest that the identified plant species, especially those with extracts showing relatively low MIC values, are playing a big role in treating skin infections in Mopani District.
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Kotina, Ekaterina L., Patricia M. Tilney, Abraham E. van Wyk, Alexei A. Oskolski, and Ben-Erik van Wyk. "“Hairy” bark in Lannea schweinfurthii (Anacardiaceae): hyperhydric-like tissue formed under arid conditions." IAWA Journal 39, no. 2 (June 13, 2018): 221–33. http://dx.doi.org/10.1163/22941932-20170197.

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ABSTRACTA remarkable, almost fur-like “indumentum” of velvety “hairs” (sometimes referred to as “fungi”) occurs on the roots (and to a lesser extent also on the trunk) ofLannea schweinfurthiivar.stuhlmanniiand is known asvhulivhadzain the Venda language (Tshivenḓa). The hairs are traditionally used by the Venda people (Vhavenḓa) of the Limpopo Province of South Africa, for various biocultural purposes. A detailed anatomical study of the origin, structure and development of these unusual “hairs” showed that they are of peridermal origin and develop from dense clusters of phelloid cells which are scattered within the stratified phellem. These cells are capable of considerable radial elongation thus forming hair-like radial files of elongated phelloid cells. The “hairy” patches on the bark may also develop from lenticels which become hypertrophied. These clusters of phelloid cells resemble the hyperhydric tissue which is reportedly formed in periderm of stems exposed to a water-saturated environment in some plant species. The formation of hyperhydric-like tissue in roots and stems ofL. schweinfurthiivar.stuhlmanniioccurs, however, under relatively arid conditions. Since this tissue contains large intercellular spaces, it may also be regarded as a specialized type of aerenchymatous phellem. The adaptive significance, if any, of the phelloid “hairs” remains unknown.
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Bapela, M. Johanna, Heino Heyman, Francois Senejoux, and J. J. Marion Meyer. "1H NMR-based metabolomics of antimalarial plant species traditionally used by Vha-Venda people in Limpopo Province, South Africa and isolation of antiplasmodial compounds." Journal of Ethnopharmacology 228 (January 2019): 148–55. http://dx.doi.org/10.1016/j.jep.2018.07.022.

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34

Ruwanza, Sheunesu. "The Edge Effect on Plant Diversity and Soil Properties in Abandoned Fields Targeted for Ecological Restoration." Sustainability 11, no. 1 (December 28, 2018): 140. http://dx.doi.org/10.3390/su11010140.

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Changes in biotic and abiotic factors may create opportunities for biodiversity recovery in abandoned agricultural fields. This study examined the natural/old field edge effect on plant diversity and soil properties at Lapalala Wilderness in Limpopo Province, South Africa. Detailed vegetation surveys and soil measurements were conducted in three old fields that share a natural/old field road edge boundary. On each site, three transects, each with four plots (10 × 10 m), located 10 m into the natural area and 10, 30 and 50 m into the old field from the edge, were setup. Plant diversity and composition measurements were conducted on each plot. Soil moisture and total N, C and P were measured at the center of each plot. Results indicate that abundance of some woody species was significantly (P < 0.001) higher close to the edge than far into the old fields. However, this was not the case for herbs and grasses which did not increase with edge proximity. All measured soil properties were significantly (P < 0.001) higher close to the edge than far into the old fields. The study concludes that both vegetation and soil properties are influenced by proximity to the edge.
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35

Muzhinji, N., J. W. Woodhall, M. Truter, and J. E. van der Waals. "Elephant Hide and Growth Cracking on Potato Tubers Caused by Rhizoctonia solani AG3-PT in South Africa." Plant Disease 98, no. 4 (April 2014): 570. http://dx.doi.org/10.1094/pdis-08-13-0815-pdn.

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Rhizoctonia solani consists of 13 anastomosis groups (AGs) designated AG1 to 13. AG3-PT is considered the predominant AG in potatoes (4) and is associated with quantitative and qualitative yield losses. Qualitative losses are typically associated with the tuber blemish disease, black scurf. However, atypical tuber blemishes such as elephant hide consisting of corky lesions on the tuber surface (2) have also been attributed to Rhizoctonia. Such atypical blemishes are not considered specific to Rhizoctonia, making direct-cause effect estimates difficult (1). Koch's postulates for the elephant hide symptom and R. solani AG3-PT have not been completed. Recently, growth cracking and scab lesions were observed on potato tubers in South Africa and attributed to a new Streptomyces species (3). These lesions and cracks were similar to elephant hide symptoms attributed to R. solani AG3-PT. Therefore, the cause of the elephant hide symptom in South Africa was investigated further. Symptoms of elephant hide and cracking have been observed on tubers from the Eastern Free State, KwaZulu-Natal, Limpopo, Mpumalanga, North-Eastern Cape, Northern Cape, North West, Sandveld, and Western Free State growing regions. In 2012, three samples of potato tubers (cv. BP1) with elephant hide and cracking were selected for analysis. These samples were collected from Clanwilliam in the Sandveld potato growing region. Tubers were surface sterilized with 1% NaOCl; sections of affected tissue were excised and plated onto potato dextrose agar (PDA). Rhizoctonia-like colonies were identified and after further sub-culturing on PDA, three representative isolates (Rh3, Rh4, and Rh6) of R. solani from each sample were obtained. For each isolate, genomic DNA was extracted and the rDNA ITS region sequenced using ITS1-F and ITS4 (2). The resulting sequences (KF234142, KF234143, and KF234144) were at least 98% identical to other AG3-PT sequences on GenBank (JX27814 and KC157664). To confirm Koch's postulates, pathogenicity tests were conducted with the three isolates. PDA plugs of each isolate were added to 10 g of barley grains which were incubated for 14 days until fully colonized. The barley grains were then used to inoculate disease-free mini-tubers (cv. BP1) in 5l pots containing a sand-clay-pine bark mixture (1:1:1 ratio). Potato plants inoculated with sterile barley grains served as controls. Plants were held for 120 days in a greenhouse at 22°C with light for 12 h a day. Incidence of the elephant hide symptom for isolates Rh3, Rh4, and Rh6 was 58%, 33%, and 37.5%, respectively. Growth cracking and black scurf were also observed with each isolate. R. solani AG3-PT was successfully re-isolated from symptomatic tubers, confirming Koch's postulates. This is the first report of R. solani AG3-PT causing elephant hide in potato tubers in South Africa. Elephant hide caused by R. solani AG3-PT has been reported in tubers from France (2) and the United Kingdom (3), but Koch's postulates were not proven. In this study, Koch's postulates were proven for R. solani AG3-PT causing scab or elephant hide symptom and cracking in potato tubers. R. solani AG3-PT should thus be considered in addition to Streptomyces as a cause of this symptom and control strategies should also consider R. solani AG3-PT. References: (1) G. J. Banville et al. Pages 321-330 in: Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control, B. Sneh et al., eds. Kluwer Academic Publishers, Dordrecht, The Netherlands, 1996. (2) M. Fiers et al. Eur. J. Plant. Pathol. 128:353, 2010. (3) R. Gouws and A. McLeod. Plant Dis. 96:1223, 2012. (4) J. W. Woodhall et al. Eur. J. Plant. Pathol. 136:273, 2013.
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36

van Niekerk, J. M., W. Bester, F. Halleen, P. W. Crous, and P. H. Fourie. "First Report of Lasiodiplodia crassispora as a Pathogen of Grapevine Trunks in South Africa." Plant Disease 94, no. 8 (August 2010): 1063. http://dx.doi.org/10.1094/pdis-94-8-1063a.

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In 2003 and 2004, a survey of grapevine (Vitis vinifera L.) trunk pathogens was conducted in 30 vineyards in the Western and Northern Cape and Limpopo provinces of South Africa. In each vineyard, 20 visually healthy plants were sampled randomly by removing the distal part of one cordon arm. Isolations were made onto potato dextrose agar (PDA) from the internal wood decay symptoms observed in the cordon samples. Seven Botryosphaeriaceae spp. were identified, including Lasiodiplodia crassispora (1). Other Botryosphaeriaceae spp. are known grapevine trunk pathogens (2). Species identity was confirmed by DNA sequence data of the partial translation factor 1-α gene (1) and sequences deposited in GenBank (GU233658 and GU233659). The L. crassispora isolates (CBS 125626 and 125627) were associated with brown internal necrosis, a known symptom of grapevine Botryosphaeriaceae spp. infection (3), in the cordon arms of Ruby Cabernet grapevines occurring in two vineyards in the Northern Cape Province. L. crassispora was described from cankered wood of Santalum album in Western Australia and endophytically from Eucalyptus urophylla in Venezuela (1). Its grapevine pathogen status was determined using both isolates in a repeated pathogenicity test that included three isolates each of Botryosphaeria dothidea and Neofusicoccum australe as positive controls (2), Trichoderma harzianum as a nonpathogen treatment, and an uncolonized agar plug as a negative control. The Botryosphaeriaceae spp. and T. harzianum were plated on PDA and incubated at 25°C for 7 days. Lignified, 6-month-old shoots of grapevine cv. Chardonnay were excised from grapevines with internodes 4 to 6 used for inoculations. Before wounding, shoots were disinfected by submersion for 1 min in a 1 ml/liter solution of a quaternary ammonium compound (Sporekill; ICA International Chemicals (Pty) Ltd, Stellenbosch, South Africa). Twelve shoots were used for each isolate or control treatment. Wounds were made 2 mm deep on the fifth internode of the shoots with a 5-mm flame-sterilized cork borer (2,3). Wounds were inoculated with a pathogen colonized agar plug (5 mm in diameter) or an uncolonized agar plug and then covered with Parafilm (2,3). Inoculated shoots were incubated in the dark in moist chambers for 14 days at 25°C. After incubation, the bark of the shoots was peeled from the area around the wound and the lengths of any resultant lesions were measured under sterile conditions. The inoculum effect was assessed by analysis of variance and Student's t-test. Results showed that significantly (P < 0.0001) longer lesions were caused by L. crassispora (13.36 mm) compared with N. australe (9.27 mm) and B. dothidea (5.28 mm) and also significantly longer than lesions caused by the nonpathogen and negative controls (3.23 and 2.90 mm, respectively). To determine if lesions were caused by inoculated fungi, isolations were made from the tissue at the edges of the lesions by aseptically removing five 0.5 × 1 mm pieces of wood and placing them on PDA dishes amended with 0.04 g/liter of streptomycin sulfate. Dishes were incubated under normal fluorescent light at 25°C for 14 days before identifying isolated fungi based on morphological and cultural characteristics (1). To our knowledge, this is the first report of L. crassispora as a grapevine pathogen. References: (1) T. I. Burgess et al. Mycologia 98:423, 2006. (2) J. M. van Niekerk et al. Mycologia 96:781, 2004. (4) J. M. van Niekerk et al. Phytopathol. Mediterr. 45:S43, 2006.
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Braack, Leo, Riana Bornman, Taneshka Kruger, Yael Dahan-Moss, Allison Gilbert, Maria Kaiser, Shüné V. Oliver, et al. "Malaria Vectors and Vector Surveillance in Limpopo Province (South Africa): 1927 to 2018." International Journal of Environmental Research and Public Health 17, no. 11 (June 9, 2020): 4125. http://dx.doi.org/10.3390/ijerph17114125.

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Despite the annual implementation of a robust and extensive indoor residual spraying programme against malaria vectors in Limpopo Province (South Africa), significant transmission continues and is a serious impediment to South Africa’s malaria elimination objectives. In order to gain a better understanding regarding possible causes of this residual malaria, we conducted a literature review of the historical species composition and abundance of malaria vector mosquitoes in the Limpopo River Valley region of the Vhembe District, northern Limpopo Province, the region with the highest remaining annual malaria cases in South Africa. In addition, mosquito surveys were carried out in the same region between October 2017 and October 2018. A total of 2225 adult mosquitoes were collected using CO2-baited tent and light traps, human landing catches and cow-baited traps. Of the 1443 Anopheles collected, 516 were members of the An. gambiae complex and 511 An. funestus group. In the malaria endemic rural areas outside the Kruger National Park, one specimen each of An. gambiae s.s. and An. funestus and only three of An. arabiensis were collected. The latter species was abundant at a remote hot spring in the neighboring Kruger National Park. Eighteen other species of Anopheles were collected. Our survey results support the historical findings that An. arabiensis, the species widely held to be the prime malaria vector in South Africa, is a rare species in the malaria endemic Limpopo River Valley. The implications of the mosquito surveys for malaria transmission, elimination and vector control in northern Limpopo Province and neighboring regions are discussed.
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38

Hlongwane, Zabentungwa T., Rob Slotow, and Thinandavha C. Munyai. "Indigenous Knowledge about Consumption of Edible Insects in South Africa." Insects 12, no. 1 (December 31, 2020): 22. http://dx.doi.org/10.3390/insects12010022.

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Consumption of edible insects is an indigenous practice that has played an essential role in human nutrition across Africa. The traditional use of insects forms an important part of food culture in Africa, and insects are consumed either as a delicacy, emergency, or staple source of food. However, indigenous knowledge about insect consumption is being lost because recent generations have adopted western methods and paid less attention to traditional practices. We conducted 500 questionnaires in five local municipalities in Kwazulu-Natal (KZN), and 122 questionnaires in four local municipalities in Vhembe district in Limpopo, South Africa, to document indigenous knowledge about edible insects’ consumption, collection, and preparation methods used in Limpopo and KZN. Eight insect species belonging to five insect orders were used as food in Limpopo and KZN, with mopane worms (94%) and termites (70%) being the most preferred species by respondents in Limpopo and KZN, respectively. Ninety-five percent of the respondents occasionally consumed insects in Limpopo, while only 28% did so in KZN. Nutritional benefits and tradition were the main reasons for consuming insects. Edible insects are a nutritious diet and play an important role in people’s livelihoods in rural areas. However, there was a notable decline in entomophagy, particularly in KZN. The decline may be related to occidental acculturation, discomfort associated with eating insects, and declining insect availability. To promote entomophagy, the authorities should encourage people to include edible insects in their diets because of their nutritional value. In addition, edible insect flour should be incorporated in food products such as biscuits, bread, energy bars, cereal, and cookies to promote acceptability.
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Mnisi, Peral, and Susan M. Dippenaar. "A report of the free-living freshwater planktonic copepods from an ephemeral pool in Limpopo Province, South Africa, with the description of Microcyclops raynerae n. sp." Crustaceana 92, no. 5 (May 3, 2019): 555–75. http://dx.doi.org/10.1163/15685403-00003894.

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Abstract Ephemeral pools are biologically important habitats for species adapted to survive through a dry phase. Unfortunately, these pools have been neglected in studies. Copepods are essential in aquatic habitats mainly because of their importance in the food web. Currently there are only 34 species reported from freshwater habitats in South Africa. This study was conducted in an ephemeral pool (Limpopo Province, South Africa). Qualitative sampling was done monthly (December 2014 to June 2015) using a plankton net and specimens were studied through a light microscope. Seven species were collected, three from Calanoida and four from Cyclopoida, including a new species, Microcyclops raynerae. Seven species represent quite a high biodiversity of copepods from a single pool when compared with similar studies previously done in South Africa. The report of Tropocyclops confinis constitutes a new geographical record from South Africa while all other collected species are new records from the Limpopo Province.
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Jaarsveld, Ernst J. Van. "Kalanchoe waterbergensis, A new Kalanchoe species from the Waterberg, Limpopo Province, South Africa." Bradleya 35, no. 35 (August 31, 2017): 166–70. http://dx.doi.org/10.25223/brad.n35.2017.a16.

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41

Klopper, R. R., and A. E. Van Wyk. "Pteridophyta–Sinopteridaceae." Bothalia 41, no. 1 (December 13, 2011): 204–7. http://dx.doi.org/10.4102/abc.v41i1.51.

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42

Mphahlele, Morutse, Ana M. Tsotetsi-Khambule, Rebone Moerane, Dennis M. Komape, and Oriel M. M. Thekisoe. "Anthelmintic resistance and prevalence of gastrointestinal nematodes infecting sheep in Limpopo Province, South Africa." Veterinary World 14, no. 2 (February 2, 2021): 302–13. http://dx.doi.org/10.14202/vetworld.2021.302-313.

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Background and Aim: Previous studies recorded the prevalence of gastrointestinal nematodes (GIN) in Limpopo Province. However, the studies did not address the seasonal patterns of infection and did not cover all districts of Limpopo Province, namely; Capricorn, Sekhukhune, Waterberg, Mopani, and Vhembe. It is, therefore, important to provide up to date information on the prevalence and seasonal occurrence data of GIN in all districts of Limpopo province. The present study was conducted to determine the occurrence of anthelmintic resistance (AR) and document the prevalence of GIN infecting sheep in five districts of Limpopo Province, South Africa. Materials and Methods: Forty animals in each district were used for fecal egg count reduction test (FECRT) to determine AR against ivermectin (0.2 mg/kg), levamisole (LEV) (5 mg/kg), and albendazole (7.5 mg/kg). Egg hatch test (EHT) was used to determine AR against thiabendazole (TBZ) and micro-agar larval development test (MALDT) was used for both TBZ and LEV. Naturally, infected sheep (n=780) were sampled for prevalence across five districts of Limpopo. FAMACHA© eye-color score estimations were also performed for each study animal. Results: FECRT showed occurrence of AR in most of the districts and a few with suspected resistance. EHT results showed AR development against TBZ for all districts, while the MALDT showed no AR against LEV in all districts, but detected AR against TBZ in Sekhukhune, Capricorn, and Waterberg. Haemonchus contortus was the most resistant species. A high nematode prevalence (88-100%) and 1210-1861 eggs per gram (EPG) was observed in all districts during the hot wet season, decreasing to 75-80% (453-1202 EPG) during the cold dry season. The sheep revealed a FAMACHA© mean score of 3, indicating mild anemia during the hot wet season except for Vhembe district that revealed a FAMACHA© mean score of 4 during the hot wet season, indicating anemia. Conclusion: AR recorded in Limpopo Province may be due to under-dosing caused by lack of weighing equipment and high treatment frequencies due to lack of proper training on anthelmintic use. The detection of AR in Limpopo is an important finding because it will help in outlining effective management systems against GIN.
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Jordaan, M., and H. M. Steyn. "Rubiaceae." Bothalia 42, no. 2 (December 9, 2012): 204–9. http://dx.doi.org/10.4102/abc.v42i2.21.

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Chauke, MA, LJ Shai, MA Mogale, MP Tshisikhawe, and MP Mokgotho. "Medicinal plant use of villagers in the Mopani district, Limpopo province, South Africa." African Journal of Traditional, Complementary and Alternative Medicines 12, no. 3 (July 30, 2015): 9. http://dx.doi.org/10.4314/ajtcam.v12i3.2.

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45

SABETA, C. T., G. C. MKHIZE, and E. C. NGOEPE. "An evaluation of dog rabies control in Limpopo province (South Africa)." Epidemiology and Infection 139, no. 10 (July 7, 2011): 1470–75. http://dx.doi.org/10.1017/s0950268811001233.

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SUMMARYRabies is a prevalent and re-emerging disease in South Africa particularly in rural areas with high human densities. Outbreaks are frequently reported in the north and eastern parts of this country, probably an indication of inadequacy in the control of the disease. Following the 2005/2006 outbreak in Limpopo, we undertook an analysis of case surveillance data and genetically characterized 18 rabies viruses, all recovered from domestic dogs. Although rabies prevalence gradually declined annually from 2007, dog rabies still remains a public and veterinary health hazard in this region. Sylvatic rabies cycles are maintained by the black-backed jackal species in specific ecological conditions in the northwest of the province (Waterberg area), unlike in the north and east (Vhembe and Mopani districts, respectively), where spillover of infection between dogs and jackals is likely to predominate. Genetic analysis demonstrated that the rabies virus strain currently circulating within dog populations in Limpopo province is the same variant responsible for the 2005/2006 rabies outbreak. However, residual foci probably exist hence the observed sporadic outbreaks. These data further underline the value of continuous and sustainable dog immunization in controlling rabies.
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46

Makonya, G. M., J. B. O. Ogola, M. A. Muasya, O. Crespo, and S. B. M. Chimphango. "Physiological response for thermotolerant Chickpea (Cicer arietinum L.; Fabaceae) genotypes in Limpopo, South Africa." South African Journal of Botany 115 (March 2018): 295–96. http://dx.doi.org/10.1016/j.sajb.2018.02.073.

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Mabapa, Moshibudi Paulina, Kingsley Kwabena Ayisi, Irvine Kwaramba Mariga, Ramasela Charlotte Mohlabi, and Richard Sello Chuene. "Production and Utilization of Moringa by Farmers in Limpopo Province, South Africa." International Journal of Agricultural Research 12, no. 4 (September 15, 2017): 160–71. http://dx.doi.org/10.3923/ijar.2017.160.171.

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48

VERHEYEN, WALTER, JAN HULSELMANS, WIM WENDELEN, HERWIG LEIRS, MARCO CORTI, THIERRY BACKELJAU, and ERIK VERHEYEN. "Contribution to the systematics and zoogeography of the East-African Acomys spinosissimus Peters 1852 species complex and the description of two new species (Rodentia: Muridae)." Zootaxa 3059, no. 1 (October 14, 2011): 1. http://dx.doi.org/10.11646/zootaxa.3059.1.1.

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We revised the taxonomic status of the putative Acomys spinosissimus complex based on the comparative study of specimen collections from Tanzania, Zambia, Zimbabwe, Mozambique, DR Congo and South Africa, by means of analysis of external morphology, craniometry, enzymes, mitochondrial DNA sequences and karyology. Our results confirm that A. spinosissimus represents a complex of species with seemingly non-overlapping distribution ranges. The distribution range of A. spinosissimus appears to be restricted between the Zambesi and Limpopo Rivers, while the reinstated A. selousi (that includes A. transvaalensis) occurs further to the South (i.e. northern limit seemingly just north of the Limpopo River). The investigated populations north of the Zambezi River are morphologically and genetically distinct from A. spinosissimus and A. selousi. Based on this evidence, we described Acomys muzei sp. nov. and Acomys ngurui sp. nov., each one occurring separately along one side of the Eastern Arc Mountains. Finally, we lacked sufficient information to describe a third new species from the area north of the Zambesi River.
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Van der Linde, J. A., D. Six, M. J. Wingfield, and J. Roux. "Consideration of factors associated with Euphorbia ingens decline in the Limpopo Province of South Africa." South African Journal of Botany 76, no. 2 (April 2010): 404–5. http://dx.doi.org/10.1016/j.sajb.2010.02.049.

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Ssymank, Axel, and Kurt Jordaens. "Description of the female of Syrittosyrphus opacea Hull, 1944 (Diptera, Syrphidae, Eristalinae) with additional notes on the genus." African Invertebrates 62, no. 1 (April 23, 2021): 339–53. http://dx.doi.org/10.3897/afrinvertebr.62.61504.

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The taxonomy of Syrphidae is far from being complete in the Afrotropical Region and many species have been described from a single sex only. One of these is the enigmatic monotypic genus Syrittosyrphus Hull, 1944, of which, so far, only the male of Syrittosyrphus opacea Hull, 1944 was described from the Eastern Cape Province in South Africa. Here, we re-describe the male and describe the female. We summarise all known distribution records from South Africa (Eastern Cape, KwaZulu-Natal and Limpopo Provinces) and Zimbabwe (Vumba), of which several are new. We also provide notes on the species’ ecology.
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