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Bevilaqua, Natalia da Cunha. "Dinâmica e ação do nicosulfuron em híbridos de milho /." Botucatu, 2019. http://hdl.handle.net/11449/181829.
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Orientador: Caio Antonio Carbonari<br>Banca: Edivaldo Domingues Velini<br>Banca: Natália Corniani<br>Resumo: O herbicida nicosulfuron se destaca entre os principais utilizados em pós-emergência na cultura do milho. Dentre as tecnologias disponíveis no mercado, alguns híbridos podem ser considerados tolerantes ao nicosulfuron, e essa sensibilidade diferencial está relacionada com o ambiente, o estádio de desenvolvimento da planta e a dose de herbicida utilizada. A seletividade de herbicidas é geralmente determinada em função da fitointoxicação e do efeito do produto nas características morfológicas e fisiológicas das plantas. De modo que o objetivo do trabalho foi determinar a sensibilidade de híbridos de milho ao herbicida nicosulfuron por meio de marcadores bioquímicos. O primeiro experimento foi conduzido em casa-de-vegetação com delineamento experimental inteiramente casualizado com 4 repetições em esquema fatorial 4 x 2 (híbridos x doses), o herbicida foi aplicado nas doses 0 e 120 g i.a. ha-1 quando as plantas se encontravam no estádio fenológico V3. Foram realizadas avaliações da taxa de transporte de elétrons (ETR), fitointoxicação e altura aos 1, 3, 7 e 14 dias após a aplicação (DAA). Aos 14 DAA as plantas foram coletadas para determinação da massa seca. Dentre os híbridos utilizados selecionou-se o mais sensível e o mais tolerante para a realização de análises de determinação dos teores do ácido aminobutírico, e dos aminoácidos valina, leucina e isoleucina, nesses mesmos períodos de avaliação. O segundo experimento foi disposto em delineamento inteiramente casualizado com 5... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: Nicosulfuron stands out among the principal post-emergent herbicides used in the maize crop. Among the commercial technologies available, there are some hybrids considered tolerant to this molecule and can present a differential sensitivity, process which is related to the environment, growth stage and herbicide dose. The objective of the study was to determine the sensitivity of corn hybrids to the herbicide nicosulfuron by means of biochemical markers. The first experiment was carried out under greenhouse conditions in a completely randomized design with four replications in a factorial scheme 4 x 2 (hybrids x doses). The herbicide was applied at 0 and 120 g a.i. ha-1, when the plants were in the V3 stage. Electron transport rate (ETR), phytointoxication and plant height were evaluated at 1, 3, 7 and 14 days after treatment (DAT). Plants were collected at 14 DAT to determine its dry weight. The most sensitive and tolerant hybrids were selected to determinate the content of 2-aminobutyric acid and valine, leucine and isoleucine amino acids, in the same evaluation period. The second experiment was arranged in a completely randomized design with five replications in a factorial scheme 8 x 2 (hybrids x doses), the nicosulfuron was applied at 0 and 120 g a.i. ha-1. The experiment was conducted in different periods, for V2 and V6 maize growing stage. One day after the herbicide application, the plants were collected to determine the content of nicosulfuron, 2-aminobutyric and quinic acid, as well as valine, leucine and isoleucine amino acids. During the experiment, the ETR was evaluated at 1, 7, 10, 14, 21 and 28 DAT, and the variables plant height and phytointoxication at 7, 14, 21 and 28 DAT. At 7 DAT the chlorophyll content was determined. Plants were collected at 28 DAT to determine the shoot dry weight. In the first experiment, the collection of the plants one day after application was considered the ...<br>Mestre
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Castro, Edicarlos Batista de [UNESP]. "Dinâmica de glyphosate em plantas de eucalipto." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/131956.
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000853172.pdf: 668118 bytes, checksum: 6bb014fda576f413445f741abae4b6a9 (MD5)<br>O eucalipto é a espécie florestal mais utilizada em reflorestamento no país, em virtude do rápido crescimento das plantas, condições climáticas e tecnologias desenvolvidas. Porém, a interferência das plantas daninhas causa grandes prejuízos, decréscimo quantitativo e qualitativo da produção do eucalipto. Entre as possibilidades de manejo de plantas daninhas, o método químico é o mais utilizado, porém, poucos são os herbicidas registrados para a cultura do eucalipto, entre os quais o glyphosate é o mais usado por controlar um grande número de espécies. Existem poucos trabalhos a respeito dos efeitos diretos e indiretos do glyphosate nesta espécie florestal. Neste trabalho, clones de eucalipto urograndis foram submetidos ao contato direto com o glyphosate e indireto por meio de aplicação sobre plantas de Brachiaria decumbens, visando caracterizar o movimento do herbicida, exsudação radicular e seus efeitos na planta. Foram realizadas avaliações de intoxicação visual do eucalipto aos 7, 14 e 21 DAA e da massa da matéria seca das plantas aos 21 DAA. Também foram realizadas quantificações por cromatografia líquida de alta eficiência e espectrometria de massas (LC-MS/MS) em diferentes partes das plantas (superior e inferior) dos seguintes compostos: glyphosate, ácido amimetilfofônico (AMPA), ácido chiquímico, chiquimato-3-fosfato, fenilalanina, tirosina e triptofano. O glyphosate foi detectado nas plantas de eucalipto para os tratamentos com a aplicação direta e indireta do herbicida e houve translocação para os locais que não foram atingidos na aplicação, mesmo quando aplicado sobre a B. decumbens, entretanto o AMPA não foi. A presença de glyphosate nas plantas influenciou na alteração dos níveis de ácido chiquímico, fenilalanina, tirosina, triptofano e chiquimato-3-fosfato, além de causar níveis de intoxicação nas plantas de eucalipto e redução de ...<br>Eucalyptus is the most used forest species in reforestation in the country due to the rapid growth of plants, climatic conditions and developed technologies. However, interference by the weeds cause major damage, the quantitative and qualitative decrease in production of eucalyptus. Among the weed management possibilities, the chemical method is the most used, however, there are few herbicides registered for eucalyptus cultivation, in which glyphosate is most commonly used to control a large number of species. There are few studies regarding the direct and indirect effects of the glyphosate on this forest species. In this paper, Eucalyptus urograndis clones were subjected to direct contact with glyphosate and indirectly through application on Brachiaria decumbens plants, aiming to characterize the movement of the herbicide, root exudation and its effects on the plant. Visual evaluations were carried out through eucalyptus phytointoxication at 7, 14 and 21 DAA and of the dry mass of the plants at 21 DAA. Also, measurements were made on different parts of plants (top and bottom) of the following compounds by high-performance liquid chromatography and mass spectrometry (LC-MS / MS): glyphosate, aminomethylphosphonic acid (AMPA), shikimic acid, shikimate-3-phosphate, phenylalanine, tyrosine and tryptophan. Glyphosate was detected in eucalyptus plants for treatments with the direct and indirect application of herbicide and there was a translocation to sites that were not attain in the application, even when applied to the B. decumbens, however the AMPA was not. The presence of glyphosate on plants, influenced the change in the shikimic acid levels, phenylalanine, tyrosine, tryptophan, and shikimate- 3-phosphate, apart from causing intoxication in eucalyptus plants and reduction of dry mass. Glyphosate was detected in eucalyptus plants after application on the plants of B decumbens with low intoxicication ...
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Castro, Edicarlos Batista de 1987. "Dinâmica de glyphosate em plantas de eucalipto /." Botucatu, 2015. http://hdl.handle.net/11449/131956.
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Orientador: Caio Antonio Carbonari<br>Banca: Edivaldo Domingues Velini<br>Banca: Leonardo David Tuffi Santos<br>Resumo: O eucalipto é a espécie florestal mais utilizada em reflorestamento no país, em virtude do rápido crescimento das plantas, condições climáticas e tecnologias desenvolvidas. Porém, a interferência das plantas daninhas causa grandes prejuízos, decréscimo quantitativo e qualitativo da produção do eucalipto. Entre as possibilidades de manejo de plantas daninhas, o método químico é o mais utilizado, porém, poucos são os herbicidas registrados para a cultura do eucalipto, entre os quais o glyphosate é o mais usado por controlar um grande número de espécies. Existem poucos trabalhos a respeito dos efeitos diretos e indiretos do glyphosate nesta espécie florestal. Neste trabalho, clones de eucalipto urograndis foram submetidos ao contato direto com o glyphosate e indireto por meio de aplicação sobre plantas de Brachiaria decumbens, visando caracterizar o movimento do herbicida, exsudação radicular e seus efeitos na planta. Foram realizadas avaliações de intoxicação visual do eucalipto aos 7, 14 e 21 DAA e da massa da matéria seca das plantas aos 21 DAA. Também foram realizadas quantificações por cromatografia líquida de alta eficiência e espectrometria de massas (LC-MS/MS) em diferentes partes das plantas (superior e inferior) dos seguintes compostos: glyphosate, ácido amimetilfofônico (AMPA), ácido chiquímico, chiquimato-3-fosfato, fenilalanina, tirosina e triptofano. O glyphosate foi detectado nas plantas de eucalipto para os tratamentos com a aplicação direta e indireta do herbicida e houve translocação para os locais que não foram atingidos na aplicação, mesmo quando aplicado sobre a B. decumbens, entretanto o AMPA não foi. A presença de glyphosate nas plantas influenciou na alteração dos níveis de ácido chiquímico, fenilalanina, tirosina, triptofano e chiquimato-3-fosfato, além de causar níveis de intoxicação nas plantas de eucalipto e redução de ...<br>Abstract: Eucalyptus is the most used forest species in reforestation in the country due to the rapid growth of plants, climatic conditions and developed technologies. However, interference by the weeds cause major damage, the quantitative and qualitative decrease in production of eucalyptus. Among the weed management possibilities, the chemical method is the most used, however, there are few herbicides registered for eucalyptus cultivation, in which glyphosate is most commonly used to control a large number of species. There are few studies regarding the direct and indirect effects of the glyphosate on this forest species. In this paper, Eucalyptus urograndis clones were subjected to direct contact with glyphosate and indirectly through application on Brachiaria decumbens plants, aiming to characterize the movement of the herbicide, root exudation and its effects on the plant. Visual evaluations were carried out through eucalyptus phytointoxication at 7, 14 and 21 DAA and of the dry mass of the plants at 21 DAA. Also, measurements were made on different parts of plants (top and bottom) of the following compounds by high-performance liquid chromatography and mass spectrometry (LC-MS / MS): glyphosate, aminomethylphosphonic acid (AMPA), shikimic acid, shikimate-3-phosphate, phenylalanine, tyrosine and tryptophan. Glyphosate was detected in eucalyptus plants for treatments with the direct and indirect application of herbicide and there was a translocation to sites that were not attain in the application, even when applied to the B. decumbens, however the AMPA was not. The presence of glyphosate on plants, influenced the change in the shikimic acid levels, phenylalanine, tyrosine, tryptophan, and shikimate- 3-phosphate, apart from causing intoxication in eucalyptus plants and reduction of dry mass. Glyphosate was detected in eucalyptus plants after application on the plants of B decumbens with low intoxicication ...<br>Mestre
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Silva, Ferdinando Marcos Lima [UNESP]. "Hormesis de herbicidas em soja." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/110940.
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000791298.pdf: 1255629 bytes, checksum: 167ceaa2fd11583895b9a2aee9dab019 (MD5)<br>O efeito benéfico de subdoses de substâncias tóxicas é conhecido há muito tempo e este fenômeno é chamado de hormesis. Em agricultura, muitos são os casos de herbicidas que em doses normais causam severas injurias nas plantas, mas que em doses muito baixas promovem o crescimento de estruturas das plantas ou promovem outros efeitos benéficos, como aumento do teor de proteína em plantas. Objetivou-se com este trabalho avaliar o efeito de hormesis dos herbicidas glyphosate, amicarbazone, mesotrione, imazapic e carfentrazone em soja, além de explorar se o efeito de hormesis na soja é afetado por um pré-tratamento com baixas doses de glyphosate e mais tarde exposta as mesmas doses ou doses maiores. Para isso, três experimentos distintos foram conduzidos, sendo que no primeiro, sete doses do herbicida glyphosate (0; 1,8; 3,6; 7,2; 36; 180 e 720 g e.a.ha-1 ) foram aplicadas sobre as plantas de soja e avaliadas quanto à taxa de transporte de elétrons (ETR), acúmulo de compostos químicos alterados em função da ação do glyphosate (ácido chiquímico, ácido benzoico, ácido salicílico, AMPA, fenilalanina, tirosina e triptofano) e massa de matéria seca das plantas. No segundo experimento, foi feito um pré-tratamento das plantas com subdoses do glyphosate (0; 1,8; 3,6 e 7,2 g e.a. ha-1 ) no estádio V2 e 14 dias após esta aplicação, as plantas receberam uma segunda aplicação de diferentes doses do glyphosate (0; 1,8; 3,6; 7,2; 36; 180 e 720 g e.a. ha-1 ) e posteriormente avaliadas quanto ao acúmulo de massa de matéria seca e ácido chiquímico. No terceiro experimento, foram testadas uma completa curva de dose-resposta dos herbicidas amicarbazone, ...<br>The beneficial effect of low doses of toxic substances is known a long time and this phenomenon is called hormesis. In agriculture, there are many cases of herbicides that in normal rates cause severe injuries in plants, but in very low doses promote the growth of plant structures or other beneficial effect such as increase in the protein content in plants. The objective of this study was to evaluate the effect of hormesis of glyphosate, amicarbazone, mesotrione, imazapic and carfentrazone in soybean, in addition to exploring if the effect of hormesis in soybeans is affected by a pretreatment with low doses of glyphosate and later expose to the same doses or higher. For this, three separate experiments were carried out, and on the first one seven doses of glyphosate (0; 1.8; 3.6; 7.2; 36; 180 and 720 g a.e.ha-1 ) were applied to the soybean plants and evaluated the electron transport rate (ETR), the chemical compounds resulting from the action of glyphosate (shikimic acid, benzoic acid, salicylic acid, AMPA , phenylalanine, tyrosine and tryptophan) and hormesis effect on the dry weight of plants. The second experiment was made a pretreatment of soybean plants with low doses of glyphosate (0; 1.8; 3.6 and 7.2 g a.e.ha-1) in the V2 stage and 14 days later the plants received a second application of different doses of glyphosate (0; 1.8; 3.6; 7.2; 36; 180 e 720 g a.e.ha-1 ) and subsequently evaluated the dry weight of plants and shikimic acid. In the third experiment, was tested a full dose-response of the herbicides amicarbazone, mesotrione, imazapic and carfentrazone on soybean plants and evaluated the ETR and dry weight. The results indicated that at low doses glyphosate stimulated ETR and increased biomass. The compounds from shikimic acid pathway such as benzoic and salicylic acids had respective increases of 203 and 170% in the dose of 3.6 g a.e. ha-1of glyphosate and the amino ...
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Silva, Breno Araguaia Souza [UNESP]. "Influência de diferentes períodos de chuva após a aplicação de Clethodim, Quizalofope - P- Metilico e Haloxifope em pós emergência no controle de plantas daninhas." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/113795.
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000806747.pdf: 942149 bytes, checksum: 75e0218de7b7a904e05815a10bd22002 (MD5)<br>O trabalho teve por objetivo avaliar a influência de diferentes intervalos de tempo de chuva após a aplicação dos herbicidas clethodim, quizalofope -p- metilico e haloxifope em pós-emergência no controle de quatro espécies de plantas daninhas: B. decumbens, B. plantaginea, Cenchrus echinatus e Zea mays na cultura da soja. O delineamento experimental adotado foi inteiramente ao acaso e os tratamentos distribuídos em esquema fatorial 3 x 6 (três herbicidas e seis intervalos de tempo de chuva), totalizando dezoito tratamentos e quatro repetições, para cada espécie de planta daninha. Quatro experimentos foram conduzidos em casa de vegetação com duas plantas por vaso, no município de Pereiras, SP. As aplicações dos herbicidas foram realizadas em horários diferentes no decorrer do dia, de modo a obter os diferentes intervalos de tempo para simulação de chuva sobre as plantas. Foram realizadas avaliações de controle aos dias 3, 7, 14, 21 e 28 dias após a aplicação (DAA) dos herbicidas. Após a aplicação dos herbicidas as plantas daninhas foram submetidas a uma lâmina de chuva de 20 mm em cinco intervalos de tempo (15, 30, 60, 120 e 240 min.) e sem chuva (tratamento herbicida controle), mais o tratamento adicional sem chuva e sem aplicação de herbicida (testemunha). Ao final de cada avaliação para cada espécie vegetal foi determinado o acúmulo de biomassa das plantas. Diante do exposto verificou-se que os intervalos de chuva interferem negativamente na ação dos herbicidas para o controle da plantas e que os herbicidas quizalofope-p-metilico e haloxifope foram os que mais se destacaram no controle das plantas.<br>The study aimed to evaluate the influence of different time intervals of rainfall after the application of herbicide clethodim, haloxyfope and quizalofope-p-methyl, in post-emergence control of four weed species: B. decumbens, B. plantaginea, Cenchrus echinatus and Zea mays on soybeans. The experimental design was completely randomized and treatments arranged in a factorial scheme 3 x 6 (three herbicides and six time intervals of rain), totaling eighteen treatments and four replications for each weed species. Four experiments were conducted at a greenhouse with two plants per pot in the municipality of Pereiras, SP. Herbicide applications were made at different times during the day, in order to obtain different time intervals to simulate rain on the plants. Control evaluations at days 3, 7, 14, 21 and 28, were performed after application (DAA) of herbicides. After herbicide application the weeds were submitted to a slide of rain of 20 mm in five time intervals (15, 30, 60, 120 and 240 min) and no rain (herbicide treatment control), plus an additional treatment with no rain and without herbicide (control). At the end of each assessment for each species was determined the accumulation of plant biomass. With the results above it was found that the ranges of rain negatively interferes with the action of herbicides to control plants and herbicide quizalofope-p-methyl and haloxyfope were the most outstanding in plants control.
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Krolikowski, Valquiria [UNESP]. "Comportamento morfo-fisiológico de Commelina benghalensis em resposta a herbicidas aplicados em pós-emergência." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/124429.
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000834972.pdf: 477804 bytes, checksum: 750acca241c14ddab63e55869dabb5d2 (MD5)<br>Do gênero Commelina, as trapoerabas são consideradas plantas daninhas em todas culturas no mundo. Além de prejudicar lavouras, as trapoerabas também infestam quintas e terrenos baldios em todo o país. Dificuldades no controle de Commelina benghalensis com os herbicidas existentes estão sendo observados em campo, portanto, este trabalho teve como objetivo de avaliar o controle de C. benghalensis, após a aplicação de diferentes herbicidas em cinco estádios fenológicos. As plantas foram cultivadas em vasos de 2 L contendo substrato Bioplant e solo, sendo mantidas durante todo experimento em casa de vegetação da Universidade estadual Paulista Julio Mesquita Filho - Faculdade de Engenharia. O delineamento experimental utilizado foi inteiramente casualizado com 4 repetições, em esquema fatorial 8 x 5, ou seja 7 herbicidas em doses comerciais atrazina, carfentrazone, flumioxazin, glyphosate, metanoarseniato ácido monossódico (MSMA), nicosulfuron, paraquat e uma testemunha, aplicados em 5 estádios fenológicos ajustados à escala BBCH (duas folhas verdadeiras ou cartucho foliar não distendido - BBCH 11; três folhas verdadeiras ou cartucho foliar não distendido - BBCH 12; Primeiro caule lateral visível - BBCH 21; Dois caules laterais visíveis - BBCH 22 e início de florescimento, com vinte perfilhos - BBCH 51). Observou-se que o estádio fenológico das plantas tem influência na eficiência do controle químico. Plantas em estádios iniciais de desenvolvimento (BBCH 11 e 12) mostraram-se mais suscetíveis aos herbicidas, no entanto ocorrendo controle em sua totalidade somente com os herbicidas atrazina, carfentrazone, MSMA e paraquat. Apesar de sofrer fortes injúrias a C. beghalensis apresentou tolerância a dose aplicada nos demais tratamentos<br>The genus Commelina, the spiderwort are considered weeds in all cultures in the world. Besides harming crops, the spiderwort also infest farms and vacant lots across the country. Difficulties in control of Commelina benghalensis with existing herbicides are observed in the field, so this study aimed to assess the control of C. benghalensis, after application of herbicides in five stages. The plants were grown in pots containing 2 L Bioplant substrate and soil being maintained throughout the experiment in a greenhouse of the Paulista State University Julio Mesquita Filho - Faculty of Engineering. The experimental design was completely randomized with four replications in factorial 8 x 5, so 7 herbicides in recommended commercial rates (atrazine, carfentrazone, flumioxazin, glyphosate, metanoarseniato ácido monossodico (MSMA),, nicosulfuron, paraquat and a control applied 5 in phenological stages adjusted the BBCH scale (two true leaves or leaf cartridge not distended - BBCH 11, three true leaves or leaf cartridge not distended - BBCH 12; First stem visible side - BBCH 21; Two visible side stems - BBCH 22 and early flowering, with twenty tillers - BBCH 51). It was observed that the growth stage of the plants affects the efficiency of chemical control. Plants in the early stages of development (BBCH 11 and 12) were more susceptible to herbicides, however occurring control in its entirety only with atrazine herbicides, carfentrazone, MSMA and paraquat. Despite suffering heavy injuries C. beghalensis have tolerance compared of another treatments
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Anunciato, Vitor Muller 1991. "Efeitos do glyphosate no crescimento e reprodução de biótipos de digitaria insularis resistente ou suscetível a este herbicida /." Botucatu, 2018. http://hdl.handle.net/11449/153658.
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Orientador: Caio Antonio Carbonari<br>Banca: Edivaldo Domingues Velini<br>Banca: Leandro Tropaldi<br>Resumo: Muito já foi e ainda vem sendo estudado sobre os efeitos clássicos de dose-resposta dos herbicidas e uma outra vertente que vem surgindo são as respostas bifásicas, que incluem hormesis, caracterizada por doses baixas de um herbicida que causa estímulo e inibição em dose elevada. Assim como o estímulo pode ser benéfico na agricultura em algumas aplicações, porem quando ocorre em plantas não alvo, o efeito de hormesis pode-se tornar um problema. Nesse trabalho foram realizados quatro experimentos. Um experimento de curva dose-resposta para biótipo resistente e suscetível de Digitaria insularis, resistente ao herbicida glyphosate, avaliando-se altura das plantas, massa fresca e seca e fitotoxicidade (%) aos 7, 14 e 21 dias após a aplicação. Houve também a repetição do mesmo, um experimento com subdosagem de glyphosate para averiguar o efeito das subdosagens no florescimento, avaliando-se o número de panículas emitidos após a aplicação dos tratamentos e o quarto experimento que foi a verificação da germinação, índice de velocidade de germinação e peso de sementes provindas do terceiro experimento. O efeito de estímulo das subdoses foram constatados em todos os experimentos sendo que diferentes faixas de doses causaram o efeito de hormesis nos biótipos de D.insularis, resistente e suscetível havendo aumento de altura, massa, peso de sementes e velocidade de germinação e diminuição do tempo necessário para emissão das inflorescências. Esses efeitos podem conceder uma grande vantag... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: Currently much has been studied about the classic dose-response effects of herbicides and another aspect that is emerging are the biphasic responses, which include hormesis, a biphasic dose-response phenomenon characterized by low dose stimulation and high dose inhibition. Just as the stimulus may be beneficial in agriculture in several applications, when this stimulus occurs in non-target plants, the effect of hormesis can become a problem. In this work, a dose-response curve experiment was performed for resistant and susceptible biotypes of Digitaria insularis, resistant to the glyphosate herbicide and evaluating plant height, fresh and dry mass and phytotoxicity (%) at 7, 14 and 21 days after application. The second experiment was the repetition of the firs. An experiment with subdoses of glyphosate are performed to investigate the effect of the subdoses on flowering, evaluating the number of panicles emitted after the application of the treatments and the fourth experiment that was the verification of the germination, germination speed index and seed weight from the third experiment. The effect of subdoses stimulation was observed in all the experiments, with different dose ranges causing the stimulus in the D.insularis biotypes, resistant and susceptible, increasing height, mass, seed weight and germination speed and reduced the time required for inflorescence emission. These effects can give a great advantage to the spread of this species, helping to change the flora of weeds<br>Mestre
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Silva, Breno Araguaia Souza 1989. "Influência de diferentes períodos de chuva após a aplicação de Clethodim, Quizalofope - P- Metilico e Haloxifope em pós emergência no controle de plantas daninhas /." Botucatu, 2014. http://hdl.handle.net/11449/113795.
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Orientador: Carlos Gilberto Raetano<br>Banca: Marcelo Rocha Correa<br>Banca: Elza Alves Correa<br>Resumo: O trabalho teve por objetivo avaliar a influência de diferentes intervalos de tempo de chuva após a aplicação dos herbicidas clethodim, quizalofope -p- metilico e haloxifope em pós-emergência no controle de quatro espécies de plantas daninhas: B. decumbens, B. plantaginea, Cenchrus echinatus e Zea mays na cultura da soja. O delineamento experimental adotado foi inteiramente ao acaso e os tratamentos distribuídos em esquema fatorial 3 x 6 (três herbicidas e seis intervalos de tempo de chuva), totalizando dezoito tratamentos e quatro repetições, para cada espécie de planta daninha. Quatro experimentos foram conduzidos em casa de vegetação com duas plantas por vaso, no município de Pereiras, SP. As aplicações dos herbicidas foram realizadas em horários diferentes no decorrer do dia, de modo a obter os diferentes intervalos de tempo para simulação de chuva sobre as plantas. Foram realizadas avaliações de controle aos dias 3, 7, 14, 21 e 28 dias após a aplicação (DAA) dos herbicidas. Após a aplicação dos herbicidas as plantas daninhas foram submetidas a uma lâmina de chuva de 20 mm em cinco intervalos de tempo (15, 30, 60, 120 e 240 min.) e sem chuva (tratamento herbicida controle), mais o tratamento adicional sem chuva e sem aplicação de herbicida (testemunha). Ao final de cada avaliação para cada espécie vegetal foi determinado o acúmulo de biomassa das plantas. Diante do exposto verificou-se que os intervalos de chuva interferem negativamente na ação dos herbicidas para o controle da plantas e que os herbicidas quizalofope-p-metilico e haloxifope foram os que mais se destacaram no controle das plantas.<br>Abstract: The study aimed to evaluate the influence of different time intervals of rainfall after the application of herbicide clethodim, haloxyfope and quizalofope-p-methyl, in post-emergence control of four weed species: B. decumbens, B. plantaginea, Cenchrus echinatus and Zea mays on soybeans. The experimental design was completely randomized and treatments arranged in a factorial scheme 3 x 6 (three herbicides and six time intervals of rain), totaling eighteen treatments and four replications for each weed species. Four experiments were conducted at a greenhouse with two plants per pot in the municipality of Pereiras, SP. Herbicide applications were made at different times during the day, in order to obtain different time intervals to simulate rain on the plants. Control evaluations at days 3, 7, 14, 21 and 28, were performed after application (DAA) of herbicides. After herbicide application the weeds were submitted to a slide of rain of 20 mm in five time intervals (15, 30, 60, 120 and 240 min) and no rain (herbicide treatment control), plus an additional treatment with no rain and without herbicide (control). At the end of each assessment for each species was determined the accumulation of plant biomass. With the results above it was found that the ranges of rain negatively interferes with the action of herbicides to control plants and herbicide quizalofope-p-methyl and haloxyfope were the most outstanding in plants control.<br>Mestre
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Moraes, Carolina Pucci de 1989. "Controle e hormesis de glyphosate em Brachiaria decumbens /." Botucatu, 2016. http://hdl.handle.net/11449/143503.
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Orientador: Edivaldo Domingues Velini<br>Banca: Caio Atonio Carbonari<br>Banca: Luciano Soares de Souza<br>Resumo: O efeito de estímulo de uma característica biológica por baixas dosesde um composto considerado tóxico, mas que em altas doses tem efeito inibitório édenominado efeito hormesis. Estímulos de crescimento em plantas têm sido verificados apósa aplicação de subdoses de alguns herbicidas, incluindo o glyphosate. Nesse contexto, opresente trabalho teve por objetivo avaliar as respostas de crescimento de plantas deBrachiaria decumbens submetidas a diferentes doses de glyphosate. Foram realizados doisestudos em casa de vegetação, ambos em delineamento inteiramente casualizado (DIC). Noprimeiro estudo foram realizados três experimentos com seis repetições, sendo noexperimento 1 testada uma curva de dose-resposta com nove doses do herbicida glyphosate(0; 22,5; 45; 90; 180; 360; 720; 1440 e 2880 g e.a. ha-1). Nos experimentos dois e três foramaplicadas onze doses do herbicida glyphosate (0; 2,8125; 5,625; 11,25; 22,5; 45; 90; 180;360; 720 e 1440 g e.a. ha-1). No estudo 2 para a avaliação de subdoses com efeito hormesisrealizou-se quatro experimentos, em DIC com oito repetições e dois tratamentos (testemunhae dose de 11,25 g e.a. ha-1). Para ambos os estudos foram coletadas oito plantas de vasos semaplicação para a determinação da biomassa inicial dos experimentos. Nos experimentos doestudo 1 foram realizadas avaliações de fitointoxicação aos 0, 7, 14 e 21 dias após a aplicação(DAA), além da coleta de oito plantas por vaso de cada tratamento para determina... (Resumo completo, clicar acesso eletrônico abaixo)<br>Mestre
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Silva, Ferdinando Marcos Lima 1981. "Hormesis de herbicidas em soja /." Botucatu :, 2014. http://hdl.handle.net/11449/110940.
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Orientador: Edivaldo Domingues Velini<br>Coorientador: Stephen O.Duke<br>Coorientador: Franck E.Dayan<br>Banca: Caio Antonio Carbonari<br>Banca: Antonio Luiz Cerdeira<br>Banca: Marcelo de Almeda Silva<br>Banca: Sidnei Douglas Cavalieri<br>Resumo: O efeito benéfico de subdoses de substâncias tóxicas é conhecido há muito tempo e este fenômeno é chamado de hormesis. Em agricultura, muitos são os casos de herbicidas que em doses normais causam severas injurias nas plantas, mas que em doses muito baixas promovem o crescimento de estruturas das plantas ou promovem outros efeitos benéficos, como aumento do teor de proteína em plantas. Objetivou-se com este trabalho avaliar o efeito de hormesis dos herbicidas glyphosate, amicarbazone, mesotrione, imazapic e carfentrazone em soja, além de explorar se o efeito de hormesis na soja é afetado por um pré-tratamento com baixas doses de glyphosate e mais tarde exposta as mesmas doses ou doses maiores. Para isso, três experimentos distintos foram conduzidos, sendo que no primeiro, sete doses do herbicida glyphosate (0; 1,8; 3,6; 7,2; 36; 180 e 720 g e.a.ha-1 ) foram aplicadas sobre as plantas de soja e avaliadas quanto à taxa de transporte de elétrons (ETR), acúmulo de compostos químicos alterados em função da ação do glyphosate (ácido chiquímico, ácido benzoico, ácido salicílico, AMPA, fenilalanina, tirosina e triptofano) e massa de matéria seca das plantas. No segundo experimento, foi feito um pré-tratamento das plantas com subdoses do glyphosate (0; 1,8; 3,6 e 7,2 g e.a. ha-1 ) no estádio V2 e 14 dias após esta aplicação, as plantas receberam uma segunda aplicação de diferentes doses do glyphosate (0; 1,8; 3,6; 7,2; 36; 180 e 720 g e.a. ha-1 ) e posteriormente avaliadas quanto ao acúmulo de massa de matéria seca e ácido chiquímico. No terceiro experimento, foram testadas uma completa curva de dose-resposta dos herbicidas amicarbazone, ...<br>Abstract: The beneficial effect of low doses of toxic substances is known a long time and this phenomenon is called hormesis. In agriculture, there are many cases of herbicides that in normal rates cause severe injuries in plants, but in very low doses promote the growth of plant structures or other beneficial effect such as increase in the protein content in plants. The objective of this study was to evaluate the effect of hormesis of glyphosate, amicarbazone, mesotrione, imazapic and carfentrazone in soybean, in addition to exploring if the effect of hormesis in soybeans is affected by a pretreatment with low doses of glyphosate and later expose to the same doses or higher. For this, three separate experiments were carried out, and on the first one seven doses of glyphosate (0; 1.8; 3.6; 7.2; 36; 180 and 720 g a.e.ha-1 ) were applied to the soybean plants and evaluated the electron transport rate (ETR), the chemical compounds resulting from the action of glyphosate (shikimic acid, benzoic acid, salicylic acid, AMPA , phenylalanine, tyrosine and tryptophan) and hormesis effect on the dry weight of plants. The second experiment was made a pretreatment of soybean plants with low doses of glyphosate (0; 1.8; 3.6 and 7.2 g a.e.ha-1) in the V2 stage and 14 days later the plants received a second application of different doses of glyphosate (0; 1.8; 3.6; 7.2; 36; 180 e 720 g a.e.ha-1 ) and subsequently evaluated the dry weight of plants and shikimic acid. In the third experiment, was tested a full dose-response of the herbicides amicarbazone, mesotrione, imazapic and carfentrazone on soybean plants and evaluated the ETR and dry weight. The results indicated that at low doses glyphosate stimulated ETR and increased biomass. The compounds from shikimic acid pathway such as benzoic and salicylic acids had respective increases of 203 and 170% in the dose of 3.6 g a.e. ha-1of glyphosate and the amino ...<br>Doutor
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Krolikowski, Valquiria. "Comportamento morfo-fisiológico de Commelina benghalensis em resposta a herbicidas aplicados em pós-emergência /." Ilha Solteira, 2015. http://hdl.handle.net/11449/124429.
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Orientador: Fernando Tadeu de Carvalho<br>Banca: Edson Lazarini<br>Banca: Gustavo Pavan Mateus<br>Resumo: Do gênero Commelina, as trapoerabas são consideradas plantas daninhas em todas culturas no mundo. Além de prejudicar lavouras, as trapoerabas também infestam quintas e terrenos baldios em todo o país. Dificuldades no controle de Commelina benghalensis com os herbicidas existentes estão sendo observados em campo, portanto, este trabalho teve como objetivo de avaliar o controle de C. benghalensis, após a aplicação de diferentes herbicidas em cinco estádios fenológicos. As plantas foram cultivadas em vasos de 2 L contendo substrato Bioplant e solo, sendo mantidas durante todo experimento em casa de vegetação da Universidade estadual Paulista "Julio Mesquita Filho" - Faculdade de Engenharia. O delineamento experimental utilizado foi inteiramente casualizado com 4 repetições, em esquema fatorial 8 x 5, ou seja 7 herbicidas em doses comerciais atrazina, carfentrazone, flumioxazin, glyphosate, metanoarseniato ácido monossódico (MSMA), nicosulfuron, paraquat e uma testemunha, aplicados em 5 estádios fenológicos ajustados à escala BBCH (duas folhas verdadeiras ou cartucho foliar não distendido - BBCH 11; três folhas verdadeiras ou cartucho foliar não distendido - BBCH 12; Primeiro caule lateral visível - BBCH 21; Dois caules laterais visíveis - BBCH 22 e início de florescimento, com vinte perfilhos - BBCH 51). Observou-se que o estádio fenológico das plantas tem influência na eficiência do controle químico. Plantas em estádios iniciais de desenvolvimento (BBCH 11 e 12) mostraram-se mais suscetíveis aos herbicidas, no entanto ocorrendo controle em sua totalidade somente com os herbicidas atrazina, carfentrazone, MSMA e paraquat. Apesar de sofrer fortes injúrias a C. beghalensis apresentou tolerância a dose aplicada nos demais tratamentos<br>Abstract: The genus Commelina, the spiderwort are considered weeds in all cultures in the world. Besides harming crops, the spiderwort also infest farms and vacant lots across the country. Difficulties in control of Commelina benghalensis with existing herbicides are observed in the field, so this study aimed to assess the control of C. benghalensis, after application of herbicides in five stages. The plants were grown in pots containing 2 L Bioplant substrate and soil being maintained throughout the experiment in a greenhouse of the Paulista State University "Julio Mesquita Filho" - Faculty of Engineering. The experimental design was completely randomized with four replications in factorial 8 x 5, so 7 herbicides in recommended commercial rates (atrazine, carfentrazone, flumioxazin, glyphosate, metanoarseniato ácido monossodico (MSMA),, nicosulfuron, paraquat and a control applied 5 in phenological stages adjusted the BBCH scale (two true leaves or leaf cartridge not distended - BBCH 11, three true leaves or leaf cartridge not distended - BBCH 12; First stem visible side - BBCH 21; Two visible side stems - BBCH 22 and early flowering, with twenty tillers - BBCH 51). It was observed that the growth stage of the plants affects the efficiency of chemical control. Plants in the early stages of development (BBCH 11 and 12) were more susceptible to herbicides, however occurring control in its entirety only with atrazine herbicides, carfentrazone, MSMA and paraquat. Despite suffering heavy injuries C. beghalensis have tolerance compared of another treatments<br>Mestre
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Santos, Izabela Thais dos. "Método para estudar a dinâmica de herbicidas em palha /." Botucatu, 2019. http://hdl.handle.net/11449/183491.
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Orientador: Edivaldo Domingues Velini<br>Coorientador: Ivana Paula Ferraz Santos de Brito<br>Banca: Caio Antonio Carbonari<br>Banca: Leandro Tropaldi<br>Resumo: Com a proibição da realização de queimadas para a colheita da cana-de-açúcar, e a implantação da colheita mecanizada, foi originado o sistema de produção denominado cana crua. Nele há o acúmulo de grandes quantidades de resíduos na superfície do solo após a colheita, sendo a cultura implantada sobre essa palhada. Nesse sistema a camada de palha pode interferir na dinâmica dos herbicidas, pois é uma barreira a ser transposta até atingir o solo. Visando obter informações acerca da dinâmica de diferentes produtos, o objetivo desse trabalho foi avaliar a transposição de herbicidas simulando aplicações direto na palha e diluída na água de chuva em palha de cana- de-açúcar. O estudo realizado no NUPAM, visando determinar a dinâmica de transposição dos herbicidas atrazine, diuron, fomesafen, glyphosate, haloxyfop-p- methyl, hexazinone, indaziflam, S-metolachlor e sulfentrazone em palha de cana-de- açúcar. Utilizou-se delineamento inteiramente ao acaso, com quatro repetições, sendo a palha utilizada cortada em 0,5 x 0,5 cm e acondicionada em cápsulas de polietileno. Tendo 9 tratamentos x 3 épocas experimentais x 2 condições. Sendo a primeira condição, a simulação da chegada do herbicida na superfície da palha após a aplicação direta da calda com pipeta de repetição de 40 μL do volume de calda de cada produto por unidade experimental, enquanto na segunda condição, foi simulada a chegada do herbicida na palha após a ocorrência de chuvas, com 40 μL do volume de calda de cada produto apl... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: With the burning fires prohibition for the sugarcane harvesting, and the mechanized harvest implantation, the production system called raw cane was originated. There is accumulation of large amounts of residues on the soil surface after harvest, and the crop is implanted on this straw. In this system the straw layer may interfere with herbicide dynamics, since it is a barrier to be transposed until reaching the ground, and in order to obtain information about the dynamics of different products, the objective of this work was to evaluate the herbicides transposition simulating direct applications in straw and diluted in rainwater in sugarcane straw. The study was carried out at NUPAM, aimed to determine the transposition dynamics of the herbicides atrazine, diuron, fomesafen, glyphosate, haloxyfop-p-methyl, hexazinone, indaziflam, S- metolachlor and sulfentrazone in sugarcane straw. A completely randomized design with four replications was used, the straw being cut into 0.5 x 0.5 cm and packed in polyethylene capsules. Having 9 treatments x 3 times experimental x 2 conditions. Being the first condition, the simulation of herbicide arrival on the straw surface after the direct application of the syrup with a 40 μL repeating pipette of each product volume per experimental unit, while in the second condition, the herbicide arrival was simulated in the straw after the occurrence of rain, with 40 μL of the volume of syrup of each product applied per experimental unit during the 25 mm rainfall simulation. Soon after, 25, 50, 75 and 100 mm accumulated rain blades and 50, 75 and 100 mm were simulated for the first and second experiments, respectively. The herbicides determination in the solutions was performed by means of chromatographic analyzes using the LCMS / MS system. Data were submitted to normality and homogeneity tests and were subsequently submitted to variance analysis by the F test, with the ...<br>Mestre
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Giovanelli, Bruno Flaibam. "Seletividade de herbicidas sobre milho EnList /." Botucatu, 2019. http://hdl.handle.net/11449/183406.
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Orientador: Caio Antonio Carbonari<br>Banca: Edivaldo Domingues Velini<br>Banca: Ricardo Victória Filho<br>Resumo: A tecnologia EnList foi desenvolvida pela empresa Corteva Agriscience, e permite a aplicação dos herbicidas 2,4-D e haloxyfop-methyl sobre o milho, devido à presença do gene aad-1, além da resistência ao glyphosate e amônio glufosinato. O objetivo deste trabalho foi avaliar a seletividade de diferentes herbicidas sobre a cultura do milho com a tecnologia EnList. Os experimentos foram conduzidos em campo e casa de vegetação. Os herbicidas aplicados foram: haloxyfop-methyl, 2,4-D, e glyphosate. Os ensaios realizados em casa-de-vegetação foram organizados em delineamento inteiramente casualisado, constituídos por cinco repetições. Os herbicidas foram aplicados em pós-emergência (V4), e obteve-se as seguintes variáveis: taxa de transporte de elétrons do Fotossistema II (Electron Transport Rate - ETR), injúria visual, e altura aos 7, 14, 21 e 28 dias após aplicação (DAA). Aos 28 DAA, foram coletadas as plantas para a obtenção da massa seca final. Os experimentos 1 e 2 corresponderam a aplicação de todos os herbicidas acima, tanto de forma isolada como em misturas. Neste mesmo estudo, foi instalado um ensaio adicional a campo para testar os parâmetros produtivos, porém com a adição de amônio-glufosinato. Os demais experimentos constituíram-se de ensaios de dose-resposta para cada um dos três herbicidas estudados, comparando-se um material com a tecnologia EnList, e sua isolinha sem o gene aad-1. Portanto, os ensaios 3 e 4 se referiram a aplicação de doses crescentes de 2,4-D, que f... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: The EnListTM traits were developed by Corteva Agriscience, and provide both 2,4-D and haloxyfop-methyl resistance for maize due to the aad-1 gene. In addition, it presents glyphosate and ammonium-glufosinate resistance. The objective of this research was to assess the selectivity of different herbicides for EnListTM maize. The experiments were conducted both in the field and in greenhouse. The herbicides applied were: haloxyfop-methyl, 2,4-D, and glyphosate. The greenhouse treatments were arranged in a completely randomized design and consisted of five replications. The herbicides were applied in post-emergence (V4), and the variables obtained were: Electron transport rate of photosystem II (Electron Transport Rate - ETR), visual injury, and plant height at 7, 14, 21 and 28 days after application (DAA). At 28 DAA, the plants were cut for obtaining the final dry mass. The experiments 1 and 2 corresponded to the application of all the herbicides cited above, both alone and in mixtures. In this same study, an additional field trial was conducted for testing the production parameters, adding ammonium-glufosinate to some treatments. The other experiments consisted of dose-response assays for each of the herbicides cited, comparing a material with the EnListTM technology, and its isoline (non aad-1 material). The experiments 3 and 4 referred to increasing doses of 2,4-D application, which were: 0; 456; 912; 1.824; 3,648 and 7,296 g a.e ha-1. Experiments 5 and 6 corresponded to the following doses of haloxyfop-methyl: 0; 63; 126; 252; 504 and 1.008 g a.i ha-1, and in addition to the variables above, the total lipid content was analyzed by ethereal extract analysis. In experiment 7, six doses of glyphosate (0; 480; 960; 1,920; 3,840; 5,760 g a.a ha-1) were tested for each material. In the first study, the applications did not result on visual injuries or decrease of the other parameters, such as yield ...<br>Mestre
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Anderson, Annette. "The effects of acetolactate synthase (ALS) inhibiting herbicides on the growth, yield, nodulation and nitrogen fixation of selected legumes." Title page, contents and abstract only, 2001. http://web4.library.adelaide.edu.au/theses/09PH/09pha545.pdf.
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Bibliography: leaves 251-268. The main aim of this thesis is to determine the effects of sulfonylurea residues and in-crop usage of selected ALS-inhibiting herbicides on the growth, yield, nodulation and nitrogen fixation of legumes under alkaline conditions. Reviews literature concerning ALS-inhibiting herbicides, nitrogen fixation and the effects of herbicides on legumes, rhizobia, nodulation and nitrogen fixation; Glasshouse trial investigating the effects of flumetsulam on biomass and nodulation of chickpea; Field trail investigating the effects of sulfonylurea residues and 'in crop' usage of flumetsulam on medic shoot biomass and seed yield and the effects of chlorsulfuron residues and 'in crop' usage of flumetsulam and imazethapyr on chickpea shoot biomass, grain yield and nitrogen fixation; develops methodologies for experiments investigating the effects of ALS-inhibiting herbicides on nodulation of chickpea.
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Macedo, Gabrielle de Castro. "Crescimento, desenvolvimento e produtividade de arroz submetido a baixas doses de glyphosate /." Botucatu, 2018. http://hdl.handle.net/11449/154314.
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Orientador: Caio Antonio Carbonari<br>Banca: Edivaldo Domingues Velini<br>Banca: Marcelo de Almeida Silva<br>Banca: Natália Corniani<br>Banca: Marcelo Rocha Corrêa<br>Resumo: O emprego de substâncias tóxicas as plantas, como o glyphosate, em doses muito menores que as recomendadas comercialmente pode ocasionar alterações em varáveis como crescimento, acúmulo de biomassa, altura, conteúdo de proteína e resistência a doenças. Desta forma, os objetivos deste trabalho foram realizar estudos para uma melhor compreensão das alterações bioquímicas, fisiológicas e fenológicas do arroz causadas por subdoses de glyphosate. O experimento 1 foi realizado à campo, na Fazenda Experimental Lageado, pertencente à Faculdade de Ciências Agronômicas, FCA/UNESP, Botucatu/SP, nos anos agrícolas de 2015/2016 e 2016/2017. O delineamento experimental utilizado foi o de blocos casualizados, arranjado em esquema fatorial 2x7, sendo o primeiro fator época de aplicação do glyphosate (V3 ou R1) e, o segundo, as doses do herbicida (0; 7,5; 15; 30; 60; 120 e 240 g e.a. ha-1), com quatro repetições. Foram avaliados a produtividade, o comprimento de panículas, número de perfilhos por m², peso de 1000 grãos, número de grãos por panícula, a fertilidade de espiguetas e o número de dias entre a aplicação e o início de cada fase fenológica. Os experimentos 2 e 3 foram realizados em casa de vegetação, no Núcleo de Pesquisas Avançadas em Matologia (NUPAM), pertecentente à Faculdade de Ciências Agronômicas, FCA/UNESP, Botucatu/SP. O experimento 2 seguiu delineamento inteiramente casualizado em esquema fatorial (7x3) com cinco repetições. Os tratamentos foram constituídos por sete doses d... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract:<br>Doutor
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SATTI, MOHAMED AHMED EISA. "THE MECHANISM OF ACTION OF FLUAZIFOP-BUTYL, HALOXYFOP-METHYL, AND SETHOXYDIM HERBICIDES ON WHEAT (TRITICUM AESTIVUM L.) SEEDLINGS (CHLOROPLAST DEVELOPMENT)." Diss., The University of Arizona, 1985. http://hdl.handle.net/10150/188097.
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Haloxyfop-methyl 2-[4-[ [3-chloro-5-(trifluoromethyl)-2-pyridinyl] oxy] phenoxy] propanoate, fluazifop-butyl (±)-2-[4-[ [5-(trifluoromethyl)-2-pyridinyl] oxy] phenoxy] propanoate, and sethoxydim 2- [1-(ethoxyimino)butyl]-5- [2-(ethylthio)propyl] -3-hydroxy-2-cyclohexen-1-one modified chloroplast pigment, growth, anatomy, and ultrastructure of pre- and postemergent-treated wheat (Triticum aestivum 'Pavon 76') seedlings. Seedlings were grown under 16 h photoperiod (105 μE/M²/S) at 32/24 C day/night temperature. Pigment, growth and anatomical studies were made on pre- and postemergent-treated seedlings. Postemergent treatments were applied to 6-day-old seedlings. Carotenoid levels were reduced by 90 to 94% in preemergent-treated seedlings at 5 x 10⁻⁵ M for each herbicide. Postemergent application of 10⁻³ M haloxyfop-methyl and sethoxydim caused a reduction of 11 and 15% in carotenoid concentration than the control, respectively. Also, they produced a significant reduction in chlorophyll a and total chlorophyll concentration. Preemergent treatment with 5 x 10⁻⁶ M of each herbicide caused a reduction of 78 to 91% in leaf fresh weight and 72 to 84% reduction in root dry weight as compared to the control. Height of the seedlings was reduced by 79 to 86% of the control. Postemergent treatments with 10⁻³ M of each herbicide reduced the length of the second leaf by 98%, whereas length of the first leaf was reduced by 36% following haloxyfop-methyl treatment and 45% following fluazifop-butyl or sethoxydim treatments. Fresh weight of leaf tissue treated with 10⁻³ M of each herbicide was reduced by 40%. Preemergent treatment of the seedlings with 3 x 10⁻⁶ and 5 x 10⁻⁶ M haloxyfop-methyl or with 5 x 10⁻⁶ M fluazifop-butyl or sethoxydim caused 35 to 50% enlargement of mesophyll cells as compared to the control. These cells appeared to contain less cytoplasm. Post- emergent application of 10⁻³ M of each herbicide caused swelling of mesophyll cells. Ultrastructural studies showed that plastids of seedlings germinated in 5 x 10⁻⁶ M of each herbicide were disrupted, swollen, and lacked internal thylakoids but contained masses of plastoglobuli. The chloroplasts of postemergent-treated seedlings had fewer granal and intergranal thylakoids, but the injury to plastids was less severe than in preemergent treatments.
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Clifford, Philip A. (Philip Alan). "Validation of a Coupled Herbicide Fate and Target Plant Species Effects Model." Thesis, University of North Texas, 1989. https://digital.library.unt.edu/ark:/67531/metadc332422/.
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A series of experiments provided data to parameterize and validate a coupled herbicide fate and target plant species effects model. This simulation model is currently designed to predict responses of water hyacinth populations to treatments of the dimethylamine formulation of 2,4- dichloro-phenoxy acetic acid (2,4-D -DMA). Experiments investigated 1) the response of water hyacinth to varying exposures of 2,4-D (DMA); 2) the role of water hyacinth density and herbicide interception in treatment effectiveness using 2,4-D (DMA); and 3) the importance of root exposure to obtain control of water hyacinth using 2,4- D (DMA). Results demonstrated the importance of leaf or canopy interception of 2,4-D (DMA) sprays in obtaining control of water hyacinth populations. The critical threshold plant tissue concentration of 2,4-D (DMA) required to elicit maximum mortality (98%) was estimated to be approximately 12 mg 2,4-D per kg water hyacinth tissue (wet weight). Root uptake apparently plays little or no role in the effectiveness of this herbicide for controlling water hyacinth growth. Validation trials illustrated the efficacy of the current model. The model was validated with data from a field operation. This research has provided considerable insight into optimal use of this auxin-type herbicide for control of water hyacinth, a monocotyledon.
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Xavier, Elouize. "Mecanismos de resistência e resposta aos herbicidas inibidores da ALS e da PROTOX em Euphorbia heterophylla L." Universidade Tecnológica Federal do Paraná, 2014. http://repositorio.utfpr.edu.br/jspui/handle/1/858.
Full textAbstract:
Euphorbia heterophylla (EPHHL) é uma importante planta daninha na agricultura
mundial, e a resistência aos herbicidas dificulta ainda mais o seu controle. Assim,
conhecer o nível de resistência, os seus mecanismos e a persistência destas
populações resistentes nas lavouras é fundamental para o estabelecimento de
estratégias de prevenção e manejo. Foram realizados experimentos, objetivando
estudar a resistência de EPHHL aos herbicidas inibidores da ALS e PROTOX nos
níveis bioquímico, de planta e populacional. O capítulo I objetivou confirmar a
ocorrência de resistência aos inibidores da ALS e determinar se existe resistência
cruzada aos herbicidas dos grupos químicos das imidazolinona, sulfoniluréia,
pirimidil-benzoato e sulfonanilida em biótipos de EPHHL com resistência múltipla
(ALS e PROTOX). O capítulo II objetivou confirmar a existência de novos biótipos de
EPHHL com resistência a PROTOX nos estados do Paraná e Rondônia e determinar
se existe resistência cruzada aos herbicidas dos grupos químicos difeniléteres,
ftalamida, triazolinona, oxadiazol e pirimidinedione. O capítulo III objetivou comparar a atividade da enzima ALS de biótipos com resistência múltipla com biótipo
suscetível, na ausência e na presença dos herbicidas inibidores enzimáticos imazapyr, imazethapyr e nicosulfuron. O capítulo IV objetivou caracterizar as atividades das enzimas peroxidase, catalase e superóxido dismutase, em biótipos de EPHHL suscetível e com resistência múltipla, pela aplicação de herbicidas inibidores da PROTOX. E o capítulo V objetivou identificar a persistência de populações resistentes aos inibidores da ALS de EPHHL em lavouras da região Sudoeste e Oeste do Paraná que apresentavam resistência em levantamento prévio e verificar a existência de resistência de EPHHL ao glifosato nestas lavouras. Os resultados destes capítulos confirmaram que os biótipos de EPHHL Bom Sucesso do Sul, Vitorino, Vilhena e Medianeira, com suspeita de resistência múltipla aos inibidores da ALS e PROTOX, apresentam a resistência aos herbicidas inibidores da ALS e da PROTOX e esta sendo cruzada aos 4 grupos químicos dos inibidores da ALS e aos 5 grupos químicos dos inibidores da PROTOX, testados. A resistência dos biótipos de EPHHL aos inibidores da ALS é atribuída à menor sensibilidade da enzima a estes herbicidas. A atividade das enzimas antioxidantes superóxido dismutase, peroxidase e catalase, para os biótipos resistentes e suscetível é dependente do herbicida e da dose utilizada e pode ser um dos mecanismos envolvidos na
resistência aos inibidores da PROTOX. Os biótipos de EPHHL resistentes aos herbicidas inibidores da ALS permanecem nas áreas mesmo após longo período de constatação da resistência. Plantas de EPHHL com resistência aos inibidores da PROTOX também permanecem nas áreas, no entanto em menor frequência.<br>Euphorbia heterophylla (EPHHL) is an important weed species in world agriculture
and its control becomes difficult due to herbicide resistance. To develop proper
EPHHL management strategies it is important to know the level and the mechanisms
of resistance and persistence of resistant populations in the fields. Experiments were
carried out to study the resistance of EPHHL to ALS and PROTOX inhibitors at
biochemical, plant and population levels. The objectives on chapter I are to confirm
the occurrence of resistance to ALS inhibitors and to determine whether there is
cross resistance to other chemical groups, such as imidazolinone, sulfonylurea,
pyrimidinyl (thio) benzoates and triazolopyrimidines in biotypes of EPHHL with
multiple resistance (ALS and PROTOX). The objectives on chapter II are to confirm
the existence of new EPHHL biotypes with resistance PROTOX in the states of
Paraná and Rondônia and to determine whether there is cross resistance to other
chemical group, such as diphenyl ethers, N-phenyl-phthalimide, triazolinone,
oxadiazole and pyrimidinedione. The objectives on chapter III are to evaluate the
activity of the ALS enzyme from biotypes with multiple resistance and to compare it to
the enzyme activity of susceptible population in the absence and in the presence of the herbicides imazapyr, imazethapyr and nicosulfuron. The objectives on chapter IV is to characterize the activity of several antioxidant enzymes, such as peroxidase, catalase and superoxide dismutase in EPHHL biotypes treated with PROTOXinhibiting herbicides. The objective on chapter V are to identify the persistence of EPHHL populations resistant to ALS inhibitors on fields from Southwest and Western Paraná that were resistant in a previous survey and check for resistance to
glyphosate in EPHHL these crops. The results support the hypothesis that EPHHL
biotypes from Bom Sucesso do Sul, Vitorino, Vilhena and Medianeira have multiple
resistance to ALS and PROTOX inhibitors. Four EPHHL biotypes are cross-resistant
to all ALS chemical groups and five populations are cross-resistant to the PROTOX
inhibitors tested. The resistance to ALS inhibitors on the EPHHL biotypes evaluated
is attributed to the lower sensitivity of the herbicide target enzyme. The activity of all antioxidant enzymes, both on the resistant and susceptible biotypes, is dependent of the herbicide and on the dose used and can be one of the mechanisms of resistance to PROTOX inhibitors. The biotypes of EPHHL resistant to ALS-inhibiting herbicides remain in the area even after long-term observation of resistance. The resistance to PROTOX inhibitors also remains in areas, but less frequently.
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Dias, Roque de Carvalho 1990. "Seletividade de herbicidas aplicados em pós-emergência em espécies de grama /." Botucatu, 2018. http://hdl.handle.net/11449/153643.
Full textAbstract:
Orientador: Edivaldo Domingues Velini<br>Coorientador: Ivana Paula Ferraz Santos de Brito<br>Banca: Caio Antonio Carbonari<br>Banca: Leandro Tropaldi<br>Resumo: As plantas daninhas interferem nos gramados, prejudicando a sua formação, estabelecimento, qualidade e estética. Destaca-se dentre as diversas técnicas o uso de herbicidas, entretanto, são escassas as informações referentes a seletividade desses produtos em gramados. Desse modo, o objetivo desse trabalho foi avaliar a seletividade de herbicidas aplicados em pós-emergência sobre as espécies Axonopus compressus (grama São Carlos), Paspalum notatum (grama Batatais), Zoysia japonica (grama Esmeralda) Cynodon dactylon x Cynodon transvaalensis (grama Bermuda - Tifton 419). Foram realizados dois experimentos, todos em casa de vegetação e repetidos em diferentes momentos. As quatro espécies de grama foram transplantadas em vasos com capacidade de 2 L e aos 40 dias após o transplantio foram realizadas as aplicações dos tratamentos. Os tratamentos constituíram-se pela aplicação de 13 herbicidas (g i.a. ha-1): chlorimuron-ethyl (15); halosulfuron (112,5); imazapyr (125,0); metsulfuron-methyl (2,4); dicamba (288); triclopyr (600); 2,4-D (2010); clomazone nas formulações convencional e microencapsulada (1200); atrazine (1250); bentazon (600); flumioxazin (30); sulfentrazone (600), além de tratamento controle (sem aplicação de herbicida) para cada espécie estudada. O delineamento experimental utilizado foi inteiramente casualizado com quatro repetições. As avaliações da taxa de cobertura verde (TCV) das espécies de gramas foram realizadas aos 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 41, 48, 55 ... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: Weeds interfere with turfgrass, hampering their formation, establishment, quality and aesthetics. Among the several techniques, the use of herbicides stands out, however, the information regarding the selectivity of these products in turfgrass is scarce. Thus, the aim of this work was to evaluate the selectivity of herbicides applied in post-emergence on the species Axonopus compressus (broadleaf carpetgrass), Paspalum notatum (bahiagrass), Zoysia japonica (japanese lawn grass), Cynodon dactylon x Cynodon transvaalensis (bermudagrass -Tifton 419). Two studies were conducted, all in a greenhouse and repeated at different times. The four turfgrass species were transplanted in vessels with a capacity of 2 L and at 40 days after transplanting the applications of the treatments. The treatments consisted of the application of 13 herbicides (g i.a. ha-1): chlorimuron-ethyl (15); halosulfuron (112.5); imazapyr (125.0); metsulfuron-methyl (2.4); dicamba (288); triclopyr (600); 2,4-D (2010); clomazone in conventional and microencapsulated formulations (1200); atrazine (1250); bentazon (600); flumioxazin (30); sulfentrazone (600), in addition to control treatment (without herbicide application) for each species studied. The experimental design was completely randomized with four replicates. The green cover rate (TCV) evaluations of the turfgrass species were performed at 0, 3, 6, 9, 12, 15, 18, 21, 24, 27, 41, 48, 55 and 62 days after application (DAA); and height and phytointoxication, in the same TCV period, except for 0 DAA. The levels of total chlorophyll and carotenoids were evaluated at 9, 18 and 27 DAA and dry biomass of the shavings at 27 and 62 DAA. The obtained data were submitted to analysis of variance by the F test, being the total chlorophyll content and dry biomass of the chips, compared to the Tukey test (p≤0.05). On the other hand, the results of TCV, phytotoxification and herbicide ...<br>Mestre
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Marchesi, Bruna Barboza. "Efeitos de formulações e intervalos sem chuva na absorção, translocação e eficácia de Glyphosate e 2,4-D /." Botucatu, 2016. http://hdl.handle.net/11449/136291.
Full textAbstract:
Orientador: Edivaldo Domingues Velini<br>Banca: Ulisses Rocha Antuniassi<br>Banca: Pedro Luis da Costa Aguiar Alves<br>Resumo: Com o objetivo de avaliar os efeitos de formulações e de intervalos sem chuva três experimentos foram conduzidos afim de se obter informações sobre a deposição, absorção, translocação e eficácia dos herbicidas 2,4-D e glyphosate, isolados e em mistura, em plantas de Conyza canadensis. Todos os estudos foram realizados em casa-de-vegetação em delineamento inteiramente casualizado e em esquema fatorial, com 6 herbicidas (780 g e.a. ha-1 do herbicida EnlistTM; 780 + 820 g e.a. ha-1 da mistura formulada EnlistDuoTM; 780 g e.a. ha-1 de DMA 806 BR; 820 g e.a. ha-1 de Glizmax®Prime; 780 + 820 g e.a. ha-1 da mistura dos herbicidas DMA 806 BR + Glizmax®Prime e 780 + 820 g e.a. ha-1 da mistura de EnlistTM + Glizmax®Prime). Para o estudo de absorção o fatorial foi 6X4, com os 6 herbicidas e 4 intervalos de lavagem, com 5 repetições; para os de deposição e translocação o fatorial foi 6X2, sendo os 6 herbicidas e 2 regiões (ápice e base), com 6 repetições; e para o de eficácia, 6X5, os 6 herbicidas e 5 intervalos de chuva, com 4 repetições. No estudo de absorção foram quantificados os teores dos herbicidas internos e externos às plantas. Nos de deposição e translocação a quantificação dos herbicidas internos às plantas foi realizada para ambos, sendo que no de translocação a quantificação foi realizada aos 2 e 4 dias após a aplicação (DAA). No de eficácia foi realizada a avaliação visual de fitointoxicação aos 7, 14, 21 e 28 DAA e a massa seca aos 28 DAA. A mistura de 2,4-D e glyphosate, sendo ela formulada ou em tanque, aumentou a absorção e a eficácia do 2,4-D no controle de Conyza canadensis. A absorção de 2,4-D foi contínua ao longo das primeiras 24 horas com aumento dos teores internos e da porcentagem de controle com o avanço dos intervalos sem chuva ou lavagem. As folhas da base de Conyza canadensis receberam as maiores deposições de 2,4-D ...<br>Abstract: With the objective to evaluate the effects of formulations and rainless intervals three experimentes were done in order to obtain information about deposition, absorption, translocation and efficacy of the herbicides 2,4-D and glyphosate, alone or in mixture, in Conyza canadensis plants. All studies were performed in green house in a completely randomized design and in a factorial with 6 herbicides (780 g a.i. ha-1 of the herbicide EnlistTM; 780 + 820 g a.i. ha-1 of the formulated mixture EnlistDuoTM; 780 g a.i. ha-1 of DMA 806 BR; 820 g a.i. ha-1 of Glizmax®Prime; 780 + 820 g a.i. ha-1 of the herbicides mixtures DMA 806 BR + Glizmax®Prime and 780 + 820 g a.i. ha-1 of the mixture EnlistTM + Glizmax®Prime). To the absorption study the fatorial was 6X4, with 6 herbicides and 4 washing intervals, with 5 repititions; to the deposition and translocation study, the factorial was 6x2, with 6 herbicides and 2 regions (apex and base), with 6 repetitions; and to the efficacy study, 6X5, with the 6 herbicides and 5 rain intervals, with 4 repetitions. In the absorption study were quantified the levels of internal and external herbicides to plants. In the deposition and translocation, quantifying the internal herbicides to plants was conducted for both and to translocation the quantification performed at 2 and 4 days after application (DAA). For the efficacy study it was conducted a visual evaluation of injury at 7, 14, 21 and 28 DAA and dry mass at 28 DAA. The mixture of 2,4-D and glyphosate, formulated or in a tank mix, increased the absorption and the efficacy of 2,4-D in control of Conyza canadensis. The 2,4-D absorption was uniform throughout the first 24 hours with increasing internal levels and the percentage of control with the advance of intervals without rain or washing. The base leaves of Conyza canadensis received the biggest deposition of 2,4-D and glyphosate, mainly responsible for the ...<br>Mestre
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Mansooji, Ali Mohammad. "Herbicide resistance in wild oats, Avena spp." Title page, contents and abstract only, 1993. http://web4.library.adelaide.edu.au/theses/09PH/09phm289.pdf.
Full text
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Maneechote, Chanya. "Mechanisms of herbicide resistance in wild oats (Avena spp.)." Title page, contents and abstract only, 1995. http://web4.library.adelaide.edu.au/theses/09PH/09phm274.pdf.
Full textAbstract:
Bibliography : leaves 159-184. This study found at least three mechanisms of resistance to the acetyl coenzyme A carboxylase (ACCase)-inhibiting herbicides. A modified target -site was responsible for moderate and high resistance to herbicides at the whole plant level. Enhanced herbicide metabolism and reduced translocation of herbicide to the target site was observed in one resistant biotype each.
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Nietschke, Brett Steven. "Integrated strategies for wild oat (Avena spp.) management in southern Australian farming systems." Title page, contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09phn677.pdf.
Full textAbstract:
Bibliography: leaves 128-146. Study was undertaken to determine the occurence and species incidence of wild oats in a major cropping region of southern Australia. Population dynamic studies were undertaken at two sites to define the seed bank decline and emergence pattern of several wild oat populations over a three year period. Management studies were conducted to determine appropriate strategies for the control of wild oats in southern Australian farming systems.
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Nascimento, Alessandro. "Eficácia de herbicidas aplicados em pré-plantio incorporado na cultura da cana-de-açúcar /." Ilha Solteira, 2016. http://hdl.handle.net/11449/134297.
Full textAbstract:
Orientador: Fernando Tadeu de Carvalho<br>Banca: Marcelo Carvalho Minhoto Teixeira Filho<br>Banca: Marco Antonio de Souza Silva<br>Resumo: Para o controle eficaz de plantas daninhas na cultura da cana-de-açúcar é pouco provável que uma única aplicação de herbicida em pré-plantio incorporado seja suficiente para manter a cultura no limpo até o seu fechamento. O objetivo do trabalho foi averiguar a eficácia (ou ineficácia) de herbicidas aplicados uma única vez em pré-plantio-incorporado para o controle de plantas daninhas em cana-de-açúcar. O delineamento experimental utilizado foi o de blocos ao acaso, com oito tratamentos e quatro repetições com parcelas de 30 m2 . A eficiência dos herbicidas no controle das plantas daninhas foi avaliada aos 30, 60, 90, 120 e 150 dias após a aplicação (DAA), por meio de uma escala visual, onde 0% = nenhum controle e 100% = controle total das plantas daninhas, considerando-se como eficiente o controle igual ou superior a 80%. Concluiu-se que todos os tratamentos (s-metolachlor a 1,5 e 3,0 L p.c./ha; smetolachlor+atrazin a 1,5 e 3,0 + 3,0 L p.c./ha; trifluralin a 2,0 L p.c./ha e trifluralin+atrazin a 2,0 + 3,0 L p.c./ha) foram altamente seletivos para a cultura da cana-de-açúcar, variedade RB 86-5453 e foram ineficazes para as dicotiledôneas Calopogonium muconoides (aos 150 DAA) e Amaranthus deflexus (aos 120 DAA) e, os tratamentos s-metolachlor+atrazin (1,5 e 3,0 + 3,0 L p.c./ha) e trifluralin+atrazin (2,0 + 3,0 L p.c./ha) foram eficientes para a espécie Panicum maximum. Todos os tratamentos proporcionaram menor crescimento e produtividade da canade-açúcar em relação à testemunha no limpo. Entre as testemunhas observou-se reduções no crescimento e principalmente na produtividade (55%) da testemunha sem capina em relação à capinada. Uma única aplicação dos herbicidas trifluralin, s-metolachlor, atrazin e suas combinações em pré-plantio incorporado, não foram suficientes para manter a cultura de canade-açúcar livre de mato-competição até o seu fechamento, indicando...<br>Abstract: To the weeds control in the sugarcane is unlikely that a single application of herbicide in preplanting-incorporated is sufficient to maintain the culture in clean until its closing. The objective of the work was to determine the effectiveness (or ineffectiveness) of herbicides applied only once in pre-plant-incorporated for weed control in sugar cane. The experimental design was a randomized block with eight treatments and four replications with plots of 30 m2 . The herbicides efficiency in weed control was assessed at 30, 60, 90, 120 and 150 days after application, through a visual scale, where 0% = no control and 100% = full control of plants weeds, considering how efficient control than or equal to 80%.It was concluded that all treatments (1.5 and 3.0 Lc.p./ha of s-metolachlor; 1.5 and 3.0 L + 3.0 Lc.p./ha of smetolachlor+atrazin; 2.0 Lc.p./ha of trifluralin and 2.0+3.0 Lc.p./ha of trifluralin+atrazin) were highly selective for the culture of sugar cane, variety RB 86-5453 and were ineffective for the dicotyledons Calopogonium muconoides (at 150 DAA) and Amaranthus deflexus (at 120 DAA) and, the treatments s-metolachlor + atrazin (1.5 and 3.0 + 3.0 Lc.p./ha) and trifluralin + atrazin (2.0 L + 3.0 c.p./ha) were effective for the species Panicum maximum. All treatments showed lower growth and yield compared to the control in the clean. Among the controls was observed reductions in growth and 55% reduction in the productivity of the treatment without controlling weeds in relation to treatment weeded. A single application of trifluralin herbicides, smetolachlor, atrazine and their combinations in corporate pre-planting, were not enough to keep the culture of sugarcane free of weed competition until its closure, indicating a need for other methods subsequent control<br>Mestre
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Nelson, J. M., D. A. Dierig, and G. L. Hart. "Effect of Planting Date, Nitrogen Fertility and Postemergence Herbicides on Lesquerella Production." College of Agriculture, University of Arizona (Tucson, AZ), 1994. http://hdl.handle.net/10150/201436.
Full textAbstract:
Lesquerella is a potential crop plant that produces seeds containing hydroxy fatty acids similar to those in castor beans. An important step in the commercialization of this plant for Arizona farms is the development of an efficient agronomic production system. Research on cultural practices for lesquerella was conducted during the 1991-92 and 1992-93 seasons. Results of planting date experiments indicate that early fall planting dates are superior to late fall or winter dates in terms of dry matter production and seed yield. Lesquerella produced a marked response to nitrogen (N) in tests comparing different N rates. In a postemergence weed control test, several herbicides gave good control of small winter weeds.
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Matos, Ana Karollyna Alves de 1991. "Uniformidade na deposição e dinâmica de formulações de diuron e sulfentrazone em solo, palha e plantas de cana-de-açúcar /." Botucatu, 2018. http://hdl.handle.net/11449/154466.
Full textAbstract:
Orientador: Caio Antonio Carbonari<br>Banca: Edivaldo Domingues Velini<br>Banca: Marcus Barifouse Matallo<br>Banca: Leandro Tropaldi<br>Banca: Natalia Corniani<br>Resumo: A mistura de herbicidas é uma prática recorrente em diversos países do mundo, e proporciona vários benefícios para o manejo de plantas daninhas. Contudo, em virtude das possíveis interações entre os herbicidas que compõem uma mistura, é necessário o conhecimento dos efeitos da mistura de herbicidas, a fim de determinar os seus possíveis efeitos nas plantas e na dinâmica dos produtos. Diante disso, o objetivo desse trabalho foi avaliar os efeitos da aplicação em mistura comercial e mistura em tanque dos herbicidas diuron e sulfentrazone, na dinâmica dos herbicidas em solos e na palha; deposição em campo, e o comportamento em variedades de cana-de-açúcar. Em todos os experimentos foi realizada a aplicação da mistura comercial (Stone®) e mistura em tanque (Herburon 500 BR® + Boral 500 SC®) dos herbicidas diuron e sulfentrazone, nas doses de 1750 g i.a. ha-1 e 875 g i.a. ha-1, respectivamente. No primeiro estudo, avaliou-se os efeitos das formulações na lixiviação dos herbicidas diuron e sulfentrazone em solos com diferentes texturas. Em cada experimento, foi utilizado um solo (textura argilosa, arenosa e média) para preencher as colunas, que após a aplicação das formulações foram submetidas a simulação de diferentes lâminas de chuva (40, 80 e 120 mm). As soluções lixiviadas foram coletadas para posterior detecção e quantificação dos herbicidas, por cromatografia líquida e espectrometria de massas (LC-MS/MS). Para o estudo de dinâmica em palha foram utilizadas cápsulas de laminad... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract:The mixture of herbicides is a recurrent practice in different countries of the world, and provides several benefits for the weed management. However, because of possible interactions between herbicides that compose a mixture, knowledge of the effects of the herbicides mixture is necessary to determine its possible effects on plants and products dynamics. Therefore, the objective of this work was to evaluate the effects of the application in commercial mixture and tank mixture of the herbicides diuron and sulfentrazone, the dynamics of herbicides in soils and straw; deposition on the field, and behavior in sugarcane varieties. In all experiments, the commercial mixture (Stone®) and tank mixture (Herburon 500 BR® + Boral 500 SC®) of the herbicides diuron and sulfentrazone were applied at doses of 1750 g a.i. ha-1 and 875 g a.i. ha-1, respectively. In the first study, were evaluated the effects of the formulations on the leaching of the diuron and sulfentrazone herbicides in soils with different textures. In each experiment, a soil (clayey, sandy and medium texture) was used to fill the columns that after the application of the formulations were submitted to simulation of different rainfalls (40, 80 and 120 mm). The leached solutions were collected for further detection and quantification of the herbicides, by liquid chromatography and mass spectrometry (LC-MS/MS). For the study of dynamics in straw were used polypropylene laminated capsules filled with sugarcane straw (equivalent to 10 tons ha-1). In the capsules submitted to the application of the herbicides was carried out in the periods 1, 15 and 30 days after application (DAA) the simulation of different rainfalls (5, 10, 20, 50 and 100 mm) and collected leachate water for further chromatographic quantification. The spray solutions used were stored for further determination of the surface tension. The deposition experiment was carried out in the ...<br>Doutor
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Diagonel, Guilherme. "Efeito de herbicidas a base de glifosato sobre as respostas fotossintéticas de macrófitas de ecossistemas lóticos tropicais /." Assis, 2019. http://hdl.handle.net/11449/182312.
Full textAbstract:
Orientador: Ciro Cesar Zanini Branco<br>Banca: Rosana Marta Kolb<br>Banca: Régis de Campos Oliveira<br>Resumo: Vários estudos observacionais sobre macrófitas de ecossistemas lóticos têm contribuído com o aumento do conhecimento ecológico destes organismos nestes ambientes. Entretanto, ainda persistem lacunas importantes neste conhecimento, entre as quais estão as relações de causa e efeito que potencialmente existem entre perturbações provocadas pela atividade antrópica e aspectos da fisiologia e da ecologia destes organismos. Com o objetivo de superar, obviamente em parte, esta lacuna, o presente estudo propõe, através de experimentos laboratoriais, avaliar os efeitos de resíduos de herbicidas usados nas lavouras agrícolas, em especial aqueles a base de glifosato (Roundup®) e seu metabólito (AMPA), sobre parâmetros fotossintéticos de espécies de angiospermas aquáticas típicas de ecossistemas lóticos tropicais. As avaliações das taxas fotossintéticas e de respiração no escuro foram realizadas através da evolução do oxigênio dissolvido, aplicando-se a técnica das garrafas claras e escuras e da fluorescência de clorofila a, que foi avaliada com o fluorômetro subaquático Diving-PAM. Os valores dos parâmetros fotossintéticos medidos/calculados para cada tratamento avaliado foram submetidos à estatística descritiva e as eventuais diferenças entre eles foram avaliadas através da análise de variância utilizando o teste de Scott-Knott e o t-Student. Foram observados alguns poucos efeitos fitotóxicos associados às espécies de macrófitas estudadas, porém, quase nenhuma diferença... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: Even with many observational studies regarding lotic ecosystems, macrophytes that have contributed with great knowledge about the ecology of these organisms in these environments. However, there are still important gaps in this knowledge, among which are the cause and effect relationships that potentially exist between disturbances caused by anthropic activity and aspects of the physiology and ecology of these organisms. With the objective of overcoming, in part, this gap, the present study proposes, through laboratory experiments, to evaluate the effects of herbicide residues used in agricultural crops, especially those based on glyphosate (Roundup®) and your metabolite (AMPA) on photosynthetic parameters of aquatic angiosperm species typical of tropical lotic ecosystems. The evaluation of photosynthetic rates and respiration in the dark were performed through the evolution of dissolved oxygen, applying the light and dark bottle technique and chlorophyll a fluorescence, which was evaluated with the Diving-PAM underwater fluorometer. The values of the photosynthetic parameters measured / calculated for each evaluated treatment were submitted to descriptive statistics and any differences between them were evaluated through analysis of variance using the Scott-Knott and t-Student test. In spite of this global pattern, species responded specificially to exposure to Roundup® and AMPA, and Egeria densa was the one that registered the greatest susceptibility to the loss of... (Complete abstract click electronic access below)<br>Mestre
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Souza, Fabiany Lilyani Gonçalves [UNESP]. "Qualidade de sementes de soja convencional e transgênica em função da dessecação das plantas com glyphosate." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/110942.
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000791255.pdf: 1126361 bytes, checksum: e2fdc0b9ded8a59775319f1afd5a8687 (MD5)<br>A máxima qualidade das sementes de soja é expressa na maturidade fisiológica, mas, a colheita nesse estádio fenológico não é recomendada, devido ao elevado teor de água nas sementes e o excesso de folhas e hastes verdes nas plantas. Com o intuito de antecipar a colheita, a prática da dessecação química, com o herbicida glyphosate, vem sendo estudada em soja e requer alguns procedimentos, visando a preservação da qualidade fisiológica das sementes no campo. Desse modo, o trabalho teve como objetivo avaliar a qualidade fisiológica de sementes de soja de cultivares convencionais e transgênicas, oriundas de plantas dessecadas com herbicida glyphosate, em diversas épocas de dessecação. A dessecação química com o herbicida glyphosate (2,0 L.ha-1) foi realizada quando os estádios fenológicos R6.0, R7.1, R7.2 e R7.3 das plantas das cultivares BRS 133, Conquista, BRS 243RR e BRS 246RR foram atingidos. As determinações e testes para avaliação da qualidade das sementes foram: teor de água, massa de 100 sementes, germinação, primeira contagem de germinação, envelhecimento acelerado, emergência de plântulas no campo, índice de velocidade de emergência de plântulas no campo, condutividade elétrica, comprimento de plântulas e massa de matéria seca de plântulas. O delineamento experimental adotado foi o de blocos casualizados, dispostos em esquema de parcelas subdivididas, com quatro repetições, totalizando 20 tratamentos. As parcelas constaram de quatro cultivares (BRS 133, Conquista, BRS 243RR e BRS 246RR) e as subparcelas corresponderam a quatro épocas de dessecação, que corresponderam a quatro estádios da cultura (R6.0, R7.1, R7.2 e R7.3) e uma testemunha para cada cultivar (sem dessecação). Os tratamentos foram dispostos em esquema fatorial com 4 cultivares x 4 épocas de dessecação, mais as testemunhas e as médias foram comparadas pelo teste de ...<br>The maximum quality of soybean seeds is expressed in physiological maturity, but, harvest is not recommended in this phase, due to the high water content in seeds and excess leaves and green stems on the plants. With the aim of anticipating the harvest, the practice of chemical desiccation, with the herbicide glyphosate, has been studied in soybean and requires some procedures, aiming at the preservation of the physiological quality of seeds in the field. In this way, the work aimed to evaluate the physiological quality of soybean seeds of conventional and transgenic cultivars from desiccated plants with herbicide glyphosate, in various periods of desiccation. Chemical desiccation with the herbicide glyphosate (2,0L.ha-1) was performed when the phenological stages R6.0, R7.1, R7.2 and R7.3 plants of cultivars BRS 133, Conquista, BRS 243RR and BRS 246RR were achieved. Tests for evaluation of the quality of the seeds were: water content, mass of 100 seeds, germination, first count of germination, accelerated aging, emergence of seedlings in the field, speed index of seedling emergence in the field, electrical conductivity, seedling length and mass of the dry matter of seedlings. The experimental design was randomized block, arranged in plots scheme subdivided, with four repetitions, totaling 20 treatments. The parcels consisted of four cultivars (BRS 133, Conquista, BRS 243RR and BRS 246RR) and the subplots corresponded to four periods of desiccation, which corresponded to four stages of culture (R6.0, R7.1, R7.2 and R7.3) and a witness to every cultivar (without desiccation). Treatments were arranged in factorial scheme 4 cultivars x 4 times of desiccation, more witnesses and the averages were compared by t test the probability 5. The application of glyphosate in phenological stages R6.0 and R7.1 of soybean plants, conventional cultivars BRS 133 and Conquista, negatively influences the physiological ...
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Souza, Fabiany Lilyani Gonçalves 1979. "Qualidade de sementes de soja convencional e transgênica em função da dessecação das plantas com glyphosate /." Botucatu :, 2013. http://hdl.handle.net/11449/110942.
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Orientador: Cláudio Cavariani<br>Banca: Edvaldo Aparecido Amaral da Silva<br>Banca: Silvio José Bicudo<br>Banca: Ricardo Augusto Dias Kanthack<br>Banca: Mariana Zampar Toledo<br>Resumo: A máxima qualidade das sementes de soja é expressa na maturidade fisiológica, mas, a colheita nesse estádio fenológico não é recomendada, devido ao elevado teor de água nas sementes e o excesso de folhas e hastes verdes nas plantas. Com o intuito de antecipar a colheita, a prática da dessecação química, com o herbicida glyphosate, vem sendo estudada em soja e requer alguns procedimentos, visando a preservação da qualidade fisiológica das sementes no campo. Desse modo, o trabalho teve como objetivo avaliar a qualidade fisiológica de sementes de soja de cultivares convencionais e transgênicas, oriundas de plantas dessecadas com herbicida glyphosate, em diversas épocas de dessecação. A dessecação química com o herbicida glyphosate (2,0 L.ha-1) foi realizada quando os estádios fenológicos R6.0, R7.1, R7.2 e R7.3 das plantas das cultivares BRS 133, Conquista, BRS 243RR e BRS 246RR foram atingidos. As determinações e testes para avaliação da qualidade das sementes foram: teor de água, massa de 100 sementes, germinação, primeira contagem de germinação, envelhecimento acelerado, emergência de plântulas no campo, índice de velocidade de emergência de plântulas no campo, condutividade elétrica, comprimento de plântulas e massa de matéria seca de plântulas. O delineamento experimental adotado foi o de blocos casualizados, dispostos em esquema de parcelas subdivididas, com quatro repetições, totalizando 20 tratamentos. As parcelas constaram de quatro cultivares (BRS 133, Conquista, BRS 243RR e BRS 246RR) e as subparcelas corresponderam a quatro épocas de dessecação, que corresponderam a quatro estádios da cultura (R6.0, R7.1, R7.2 e R7.3) e uma testemunha para cada cultivar (sem dessecação). Os tratamentos foram dispostos em esquema fatorial com 4 cultivares x 4 épocas de dessecação, mais as testemunhas e as médias foram comparadas pelo teste de ...<br>Abstract: The maximum quality of soybean seeds is expressed in physiological maturity, but, harvest is not recommended in this phase, due to the high water content in seeds and excess leaves and green stems on the plants. With the aim of anticipating the harvest, the practice of chemical desiccation, with the herbicide glyphosate, has been studied in soybean and requires some procedures, aiming at the preservation of the physiological quality of seeds in the field. In this way, the work aimed to evaluate the physiological quality of soybean seeds of conventional and transgenic cultivars from desiccated plants with herbicide glyphosate, in various periods of desiccation. Chemical desiccation with the herbicide glyphosate (2,0L.ha-1) was performed when the phenological stages R6.0, R7.1, R7.2 and R7.3 plants of cultivars BRS 133, Conquista, BRS 243RR and BRS 246RR were achieved. Tests for evaluation of the quality of the seeds were: water content, mass of 100 seeds, germination, first count of germination, accelerated aging, emergence of seedlings in the field, speed index of seedling emergence in the field, electrical conductivity, seedling length and mass of the dry matter of seedlings. The experimental design was randomized block, arranged in plots scheme subdivided, with four repetitions, totaling 20 treatments. The parcels consisted of four cultivars (BRS 133, Conquista, BRS 243RR and BRS 246RR) and the subplots corresponded to four periods of desiccation, which corresponded to four stages of culture (R6.0, R7.1, R7.2 and R7.3) and a witness to every cultivar (without desiccation). Treatments were arranged in factorial scheme 4 cultivars x 4 times of desiccation, more witnesses and the averages were compared by t test the probability 5. The application of glyphosate in phenological stages R6.0 and R7.1 of soybean plants, conventional cultivars BRS 133 and Conquista, negatively influences the physiological ...<br>Doutor
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Voss, Joshua. "Forage adaptability trials for forage and seed production in Bolivia : effect of 5 herbicides on 7 native Utah forbs /." Diss., CLICK HERE for online access, 2006. http://contentdm.lib.byu.edu/ETD/image/etd1639.pdf.
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Fajri, Abolhassan. "Nitrogen fixation by pasture legumes : effects of herbicides and defoliation." Title page, table of contents and abstract only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09phf1755.pdf.
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Bibliography: leaves 209-254. Experiments detailed in this thesis, evaluate the impact of various herbicides and herbicide mixtures on the growth, nodulation and nitrogen fixation of annual pasture legumes, the efficacy of the herbicides for weed control, and the potential role of mechanical defoliation to replace herbicides, leading to lower cost and more sustainable farming systems.
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Upham, Brad Luther. "Interactions of paraquat and nitrodiphenylether herbicides with the chloroplast photosynthetic electron transport in the activation of toxic oxygen species." Diss., Virginia Polytechnic Institute and State University, 1986. http://hdl.handle.net/10919/82614.
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The interactions of paraquat (methylviologen) and diphenylether herbicides with the Mehler reaction as investigated. Sera from two different rabbits (RS1 & RS2) were examined for their patterns of inhibition of the photosynthetic electron transport (PET) system. Serum from RS2 was greatly hemolyzed. Fifty ul of RS1 serum were required for 100% inhibition of a H₂O → methylviologen(MV)/O₂ reaction, whereas only 10 µl of a 1:10 dilution of RS2 were needed for 100% inhibition. The γ-globulin fraction from purified rabbit serum (RS1) did not inhibit PET, indicating that the antibody fraction of the rabbit serum does not contain the inhibitor. It appears that the inhibitor is from the hemolyzed red blood cells. Rabbit sera, added to chloroplast preparations prior illumination, caused no inhibition of a H₂O → MV/O₂ reaction while addition of rabbit sera during illumination inhibited the H₂O → MV/O₂ reaction within 1-3 s. Various Hill reactions were used to determine the site of inhibition. Rabbit sera inhibited photosystem I (PSI) Hill reactions, but did not inhibit a photosystem II (PSI II) Hill reaction indicating that inhibition is on the reducing side of PSI. It would be expected that a H₂O → Ferredoxin (Fd)/NADP Hill reaction should also be blocked. Surprisingly, rabbit sera did not inhibit this reaction. These results were interpreted as supportive evidence for parallel (branched) electron transport on the reducing side of PSI.
Six pyridyl derivatives {benzylviologen, 2-anilinopyridine, 1,2-bis(4-pyridyl)ethane, 1,2-bis(4-pyridyl)ethylene, 2-benzoylpyridine, and 2-benzylaminopyridine} and five heme-iron derivatives {hemoglobin, hemin, hematin, ferritin, and ferrocene} were screened for their potential to counteract paraquat toxicity on pea (Pisum sativum L. cv. Little Marvel) isolated chloroplasts. H₂O → MV/O₂ and H₂O → Fd/NADP+ were the two Hill reactions assayed with these compounds. Antagonists of paraquat toxicity should inhibit the first Hill reaction but not the latter. None of the pyridyl derivatives examined inhibited the reaction H₂O → MV/O₂. Ferritin and ferrocene were also ineffective as inhibitors of this reaction. Hemoglobin inhibited the reaction H₂O → MV/O₂ without inhibiting the reaction H₂O → Fd/NADP+, providing protection to pea chloroplasts against paraquat. Hemin and hematin inhibited both Hill reactions examined. Hemin and hematin also inhibited H₂O → diaminodurene (ox) and durohydroquinone → MV/O₂ Hill reactions but not the dichlorophenylindolphenol(red) → MV/O₂ and diaminodurene(red) → MV/O₂ Hill reactions. These results indicate that hemin and hematin are inhibiting photosynthetic electron transport in the plastoquinone pool region.
Potential involvement of hydroxyl and alkoxyl radicals in the peroxidative action of the p-nitro diphenyl ether herbicides acifluorfen was evaluated under laboratory conditions. Methional was added to illuminated pea thylakoids and its oxidation to ethylene was used as an indicator of hydroxyl and alkoxyl radical synthesis. Oxyfluorfenstimulation of the rate of methional oxidation was dependent on light, photosynthetic electron transport and hydrogen peroxide since it was not observed under dark conditions or in the presence of DCMU and catalase. Addition of FeEDTA, a catalyst of the Fenton reaction, stimulated the oxyfluorfen-induced enhancement of methional oxidation six-fold suggesting that hydroxyl radicals are synthesized through a Fenton reaction. Acifluorfen, nitrofen and nitrofluorfen inhibited the rate of methional oxidation whereas, acifluorfen-methyl had no effect on the rate of methional oxidation even at high concentrations (1 mM). Nitrofluorfen at 1 mM was the only p-nitro diphenyl ether herbicide tested which inhibited photosynthetic electron transport of pea thylakoids. In experiments with pea leaf discs, acifluorfen at low concentrations stimulated the rate of methional oxidation, while acifluorfen-methyl, nitrofen and nitrofluorfen had no effect. These data indicate that hydroxyl and alkoxyl radicals could be involved in the mechanism of cellular damage caused by oxyfluorfen, but they are not important for the activity of the diphenyl ether herbicides acifluorfen, acifluorfen-methyl, nitrofen, and nitrofluorfen.
Diethyldithiocarbamate (DEDTC) does not accept electrons from the photosynthetic electron transport (PET), but can donate electrons to a photosystem I (PSI) Mehler reaction in the presence of the following PET inhibitors: diuron, dibromothymoquinone, and bathophenanthroline. It cannot photoreduce PSI in the presence of cyanide, a PET inhibitor. These data indicate that the site of electron donation is after the plastoquinone pool. Ascorbate is not required for the ability of DEDTC to donate electrons to PSI. There is no photoreductant activity by DEDTC in a ferredoxin/NADP Hill reaction. Superoxide dismutase inhibits DEDTC/diuron or bathophenanthroline → MV/O₂ Mehler reaction. Catalase does not restore the consumed O₂ from a DEDTC/diuron → MV/O₂ Mehler reaction, indicating O₂- has not been dissmutating into H₂O₂. These results indicate that superoxide is required for DEDTC ability to donate electrons, therefore DEDTC is limited only to Mehler-type reactions.<br>Ph. D.
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Latorre, Débora de Oliveira [UNESP]. "Intoxicação e alterações metabólicas do algodão sensível e resistente ao amônio glufosinate." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/110932.
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000800283.pdf: 3261975 bytes, checksum: bdfd9509a74fed5a5ddfcc147954a0fb (MD5)<br>As cultivares de algodão Liberty Link ® são aquelas que resistem à aplicação do amônio glufosinate a uma dose que normalmente seria recomendada para os demais biótipos da espécie. Estas cultivares de algodão são constituídas geneticamente com um gene que codifica a produção da enzima fosfinotric ina acetil transferase (PAT), responsável pela acetilação do amônio glufosinate, inativando - o na planta. A cultivar de algodão FiberMax ® 975 WideStrike por também apresentar em seu DNA o gene PAT apresentaria o comportamento de seu metabolismo intermediário às cultivares convencional e Liberty Link ® . O objetivo do trabalho foi avaliar as alterações metabólicas e a intoxicação das plantas de algodão Wide Strike ® comparadas a plantas Liberty Link ® e convencionais, quando submetidos ao herbicida amônio glufosinate. Os es tudos foram conduzidos em casa de vegetação n a Faculdade de Ciências Agronômicas - FCA / UNESP - Campus de Botucatu (SP). Dois experimentos foram instalad os simultaneamente utilizand o cultivares as cultivares transgênicas e convencional , sendo o primeiro para avaliar a intoxicação e alterações na fotossíntese e o segundo para quantificar compostos relacionados a rota de ação do herbicida amônio glufosinate . Foram semeadas 5 sementes p or vaso de três cultivares de algodão FM 993 (convencional), FM 975WS ® (transgênica) e IMACD 6001LL ® (transgênica) e, aos 15 dias após a emergência (DAE) foi realizado o desbaste das plantas, mantendo duas plantas por vaso. Quando as plantas apresentaram 2 folhas verdadeiras (25 dias após emergência) realizou - se a primeira aplicação dos ...<br>The Liberty Link cotton cultivars are those that resist a dose of ammonium glufosinate application that normally would be recommended for other biotypes species .These cotton plants are constitute genetically with gene that encodes the production of enzyme phosphinothricin acetyl transferase (PAT) responsible for the acetylation of glufosinate ammonium, inactivating it in the plant. The cotton cultivar FiberMax® 975 WideStrike has present in i ts DNA the gene PAT and present intermediate metabolism to conve ntional cultivars and Liberty Link ®. The purpose of the study was to evaluate the metabolic changes and the intoxication of WideStrike®cotton plants compared with Liberty Link ® and conventional plants, when treated to ammonium glufosinate herbicide. The studies were carried out in a greenhouse at the Faculty of Agricultural Sciences, “Júlio de MesquitaFilho State University – Botucatu Campus (SP). Were conducted two experiments simultaneously using the transgenic and conventional cultivars, the first one to evaluate the toxicity and changes in photosynthesis and the secondto quantify compounds related to the herbicide ammonium glufosinate action route. Five seeds were sown of each cultivar per pot of the three cotton cultivars FM 993 (non - genetically modi fied), FM 975WS® (genetically modified) and IMACD 6001LL® (genetically modified) and at 15 days after emergence (DAE) was performed thinning of the plants, keeping two plants per pot. When the plants had two true leaves (25 days after emergence) held the ...
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Latorre, Débora de Oliveira 1986. "Intoxicação e alterações metabólicas do algodão sensível e resistente ao amônio glufosinate /." Botucatu :, 2014. http://hdl.handle.net/11449/110932.
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Orientador: Caio Antonio Carbonari<br>Coorientador: Anderson Luis Cavenaghi<br>Banca: Elza Alves Correa<br>Resumo: As cultivares de algodão Liberty Link ® são aquelas que resistem à aplicação do amônio glufosinate a uma dose que normalmente seria recomendada para os demais biótipos da espécie. Estas cultivares de algodão são constituídas geneticamente com um gene que codifica a produção da enzima fosfinotric ina acetil transferase (PAT), responsável pela acetilação do amônio glufosinate, inativando - o na planta. A cultivar de algodão FiberMax ® 975 WideStrike por também apresentar em seu DNA o gene PAT apresentaria o comportamento de seu metabolismo intermediário às cultivares convencional e Liberty Link ® . O objetivo do trabalho foi avaliar as alterações metabólicas e a intoxicação das plantas de algodão Wide Strike ® comparadas a plantas Liberty Link ® e convencionais, quando submetidos ao herbicida amônio glufosinate. Os es tudos foram conduzidos em casa de vegetação n a Faculdade de Ciências Agronômicas - FCA / UNESP - Campus de Botucatu (SP). Dois experimentos foram instalad os simultaneamente utilizand o cultivares as cultivares transgênicas e convencional , sendo o primeiro para avaliar a intoxicação e alterações na fotossíntese e o segundo para quantificar compostos relacionados a rota de ação do herbicida amônio glufosinate . Foram semeadas 5 sementes p or vaso de três cultivares de algodão FM 993 (convencional), FM 975WS ® (transgênica) e IMACD 6001LL ® (transgênica) e, aos 15 dias após a emergência (DAE) foi realizado o desbaste das plantas, mantendo duas plantas por vaso. Quando as plantas apresentaram 2 folhas verdadeiras (25 dias após emergência) realizou - se a primeira aplicação dos ...<br>Abstract: The Liberty Link cotton cultivars are those that resist a dose of ammonium glufosinate application that normally would be recommended for other biotypes species .These cotton plants are constitute genetically with gene that encodes the production of enzyme phosphinothricin acetyl transferase (PAT) responsible for the acetylation of glufosinate ammonium, inactivating it in the plant. The cotton cultivar FiberMax® 975 WideStrike has present in i ts DNA the gene PAT and present intermediate metabolism to conve ntional cultivars and Liberty Link ®. The purpose of the study was to evaluate the metabolic changes and the intoxication of WideStrike®cotton plants compared with Liberty Link ® and conventional plants, when treated to ammonium glufosinate herbicide. The studies were carried out in a greenhouse at the Faculty of Agricultural Sciences, "Júlio de MesquitaFilho" State University - Botucatu Campus (SP). Were conducted two experiments simultaneously using the transgenic and conventional cultivars, the first one to evaluate the toxicity and changes in photosynthesis and the secondto quantify compounds related to the herbicide ammonium glufosinate action route. Five seeds were sown of each cultivar per pot of the three cotton cultivars FM 993 (non - genetically modi fied), FM 975WS® (genetically modified) and IMACD 6001LL® (genetically modified) and at 15 days after emergence (DAE) was performed thinning of the plants, keeping two plants per pot. When the plants had two true leaves (25 days after emergence) held the ...<br>Mestre
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Gazola, Tiago 1987. "Ação do herbicida glyphosate em biótipos de Digitaria insularis resistentes e suscetíveis /." Botucatu, 2017. http://hdl.handle.net/11449/151595.
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Orientador: Edivaldo Domingues Velini<br>Banca: Adilson Pereira Duarte<br>Banca: Leandro Tropaldi<br>Resumo: As infestações com Digitaria insularis em áreas de culturas anuais e os casos de resistência ao herbicida glyphosate aumentaram nos últimos anos, e estes fatos contribuíram para tornar a espécie uma das mais importantes plantas daninhas do Brasil. Diante disso, foram desenvolvidos experimentos que tiveram como objetivo confirmar a resistência de biótipos de D. insularis perenizados e propagados vegetativamente resistentes ao glyphosate, bem como, compreender o perfil metabólico do herbicida nestas plantas, verificar se existe alteração no controle relativo em função dos métodos de propagação empregados e determinar o fator de resistência nas progênies obtidas por sementes autofecundadas. Foram realizados três experimentos em casa de vegetação no delineamento inteiramente casualizado, sendo os dois primeiros com oito biótipos de capim-amargoso considerados como suscetíveis e três com suspeita de resistência ao herbicida glyphosate. No primeiro experimento foi avaliado o controle das plantas aos 7, 14, 21 e 28 dias após a aplicação (DAA) do glyphosate. No segundo experimento, avaliou-se o controle nas mesmas épocas do primeiro e 72 horas após a aplicação do herbicida foi quantificado o acúmulo de ácido chiquímico e ácido quínico, deposição, absorção e translocação de glyphosate, concentração de AMPA, dos aminoácidos aromáticos (fenilalanina, tirosina e triptofano) e ácido salicílico. No terceiro experimento foram conduzidas curvas de dose-resposta com glyphosate nas doses de 0;... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: Digitaria insularis ocurrence infestations in annual crop and cases of glyphosate herbicide resistance have increased in recent years, and the facts contributed to make the species one of the most important weeds in Brazil. Therefore, experiments were carried out to confirm the resistance of perennial and vegetatively propagated D. insularis biotypes resistant to glyphosate, as well as to understand the metabolic profile of the herbicide in these plants, to verify if there is alteration in the relative control according to the methods and determine the resistance factor in the progenies obtained by self-fertilized seeds. Three experiments were carried out under greenhouse conditions in a completely randomized design, the first two with eight sourgrass biotypes considered as susceptible and three with suspected resistance to glyphosate herbicide. In the first experiment, the control of the plants at 7, 14, 21 and 28 days after glyphosate application (DAA) was evaluated. In the second experiment, the control was evaluated in the same periods of the first and 72 hours after the application of the herbicide was quantified the accumulation of shiquimic acid and quinic acid, deposition, absorption and translocation of glyphosate, concentration of AMPA, aromatic amino acids (phenylalanine, tyrosine and tryptophan) and salicylic acid. In the third experiment, dose-response curves were conducted with glyphosate at doses of 0; 90; 180; 360; 720; 1440; 2880 and 5760 g e.a. ha-1, in the... (Complete abstract click electronic access below)<br>Mestre
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Zama, Paul. "Studies on the mechanisms of action of the herbicide safener CGA- 92194." Diss., Virginia Polytechnic Institute and State University, 1985. http://hdl.handle.net/10919/49970.
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CGAr92194 {α-[1,3-dioxolan-2-yl-methoxy)imino]benzeneacetonitrile} is a herbicide safener that is used as a seed dressing agent (1.25 g ai/kg seeds) to protect grain sorghum [<i>Sorghum bicolor</i> (L.) Moench] against metolachlor [2-chloroi-<u>N</u>-(2-ethyl-6-methylphenyl-<u>N</u>-(2-methoxy1-methylethyl)acetamide] injury. The potential adverse phytotoxic effects and the mechanisms of the protective action of this safener were studied in laboratory experiments.
Adverse phytotoxicity was assessed by comparing CGA-92194 and the herbicide safeners cyometrinil {(Z)-α[(cyanomethoxy)imino]benzeneacetonitrile} and flurazole [phenylmethyl 2-chloro-4-(trifluromethyl)-5-thiazolecarboxylate] for their effects on CO₂ fixation, protein, DNA, RNA and lipid syntheses of enzymatically isolated leaf cells of soybean [<u>Clycine max</u> (L.) Merr]. At physiological concentrations of less than 10 μM, CGA-92194, cyometrinil and flurazole were stimulatory of all metabolic processes. At 100 μM, the safeners were inhibitory of the five processes with flurazole being the most potent.
The mechanisms of the safening action of CGA-92194 were studied by examining the potential interactions of this safener with metolachlor at the levels of uptake and macromolecular syntheses in enzymatically isolated leaf mesophyll protoplasts of grain sorghum. The influence of CGA-92194 on the <i>in vitro</i> reactivity of metolachlor with glutathione (GSH) and it metabolism by sorghum seedlings were also examined. When CGA-92194 and metolachlor were given simultaneously, CGA-92194 enhanced the uptake of ¹⁴C-metolachlor into the sorghum protoplasts in a concentration-dependent pattern. Thus, interference with herbicide uptake is not involved in the protective action of this safener Treatments with metolachlor and CGA-92194 in combination inhibited the incorporation of ¹⁴C-uracil, ³H-thymidine and ¹⁴C-acetate into sorghum protoplast macromolecules less than metolachlor given alone, suggesting the potential involvement of a competitive antagonism in the mechanism of action of CGA-92194.
The metabolic activity and growth of sorghum seedlings grown from CGA-92194-pretreated seeds was significantly lower than that of seedlings grown from untreated seeds at 10 or 20 days after planting, The relationship of these effects of CGA-92194 to its safening action is unclear at the present time.
CGA-92194 increased the <i>in vitro</i> chemica1 reactivity of metolachlor for GSH in a concentration-dependent pattern, Sorghum seedlings grown from safener-pretreated seeds enhanced ¹⁴C-metolachlor absorption and stimulated its metabolism <u>via</u> conjugation to GSH. This stimulation was reduced by tridiphane [2-(3,5-dichlorophenyl)-2-(2,2,2-trichloroethy1)- oxirane] a potent inhibitor of plant GSH-<u>S</u>-transference enzymes, These results indicate that a safener-induced stimulation of the spontaneous or enzymatic conjugation of metolachlor with GSH is most likely involved in the protective action of CGA-92194.
It is suggested that the safening mechanism of action of CGA—92194 involves a sequence of multilevel interactions which together contribute to the overall protection of grain sorghum from metolachlor injury.<br>Ph. D.<br>incomplete_metadata
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Dadazio, Tais Santo 1989. "Obtenção e análise de espectros de absorção de luz por herbicidas /." Botucatu, 2018. http://hdl.handle.net/11449/180635.
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Orientador: Edivaldo Domingues Velini<br>Coorientador: Leandro Tropaldi<br>Banca: Caio Antonio Carbonari<br>Banca: Fernando Tadeu de Carvalho<br>Banca: Luiz Lonardoni Foloni<br>Banca: Ferdinando Lima Silva<br>Resumo: Cada substância apresenta um padrão de resposta específico quando submetidas à radiação solar. No entanto, estudos que comparam diferentes moléculas, a fim de identificarem padrões de comportamento são escassos. Portanto, estudos que relacionam o padrão de absorbância com a radiação solar disponível, buscando estimar o potencial de fotodegradação de defensivos, são inexistentes na literatura. Nesse contexto, esse trabalho estudou a absorção de luz por diferentes herbicidas e metabólitos com o objetivo de prever a sensibilidade desses compostos à fotólise em condições de campo e a necessidade de utilizar manejos ou tecnologias que possam reduzir a degradação por efeito da luz. Foram investigadas 36 substâncias, correspondendo a herbicidas e seus metabólitos. Cada solução foi analisada por meio de varredura das absorbâncias das substâncias na faixa de 185 a 900nm (Ultravioleta (UV)/ Visível (VIS)/Infra vermelho(IF)). Posteriormente, o potencial de cada comprimento de onda em promover a fotólise de um composto específico foi determinado multiplicando-se a disponibilidade de luz no espectro solar padrão e a absorbância observada na solução padronizada desse composto no mesmo comprimento de onda. De acordo com os resultados, cada substância apresentou faixas de comprimento de ondas com maior potencial de absorção, especialmente na região do VIS e com outros estudos permitirão avaliar quais compostos podem ser utilizados para protegê-las. Assim, foi possível agrupar as substancias ... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: Each substance has a specific response pattern when subjected to solar radiation. However, studier comparing differente molecules in order do identify behavior patterns are scarce. Therefore, studies that relate the absorbance pattern to the available solar radiation, seeking to estimate the photodegradation potential of pesticides are not available in the literature. In this context, this work studied the absorption of light by different herbicides and metabolites in order to predict the sensitivity of these compounds to photolysis underf field conditions and the need to use care or technologies that can reduce light degradation. There were investigated 36 substances, corresponding to herbicides and thei metabolites. Each solution was analyzed by scanning the absorbances of substances in the range of 185 to 900 nm (UV/VIS/IF). Subsequently, the potential of each wavelength promoting the photolysis of a specific compound was determined by multiplying the light availability in the standard solar spectrum and the observed absorbance in the standard solution of that compound at the same wavelenght. According to the results, each substance showed wavelenght ranges with greater absorption potential, and with other studier, it will be possible to evaluate which compounds can be used to protect them. Thus, it was possible to group the substances in relation to the photodegradation index in descending order in the UVA, UVB and UVC radiation range. The herbicides pendimenthalin and trifluralin stand out by expressively absorbing visible light (400 to 700 nm)<br>Doutor
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Marques, Renata Pereira [UNESP]. "Seletividade de herbicidas aplicados sobre espécies de gramas em relação às características morfo-anatômicas foliares." Universidade Estadual Paulista (UNESP), 2013. http://hdl.handle.net/11449/99926.
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marques_rp_dr_botfca.pdf: 1079511 bytes, checksum: 05a89961e7b31141dc02969e2c9d9a12 (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>O objetivo desse trabalho foi avaliar a seletividade dos herbicidas bentazon, nicosulfuron, halosulfuron, oxadiazon e 2,4-D aplicados em pós-emergência sobre as espécies Axonopus compressus (grama São Carlos), Paspalum notatum (grama Batatais), Stenotaphrum secundatum (grama Santo Agostinho) e Zoysia japonica (grama Esmeralda) e relacionar a seletividade destes herbicidas com a anatomia foliar destas espécies de gramas. As quatro espécies de grama apresentavam 48 meses de idade no momento da aplicação dos tratamentos. Os tratamentos constituíram-se pela aplicação dos herbicidas bentazon (720 g ha-1), nicosulfuron (50 g ha-1), halosulfuron (112,5 g ha-1), oxadiazon (875 g ha-1) e 2,4-D (698 g ha-1), além de uma testemunha sem aplicação, para cada espécie avaliada. O delineamento experimental utilizado foi em blocos ao acaso com quatro repetições. As avaliações de fitotoxidade das espécies de gramas foram realizadas a cada 7 dias após a aplicação dos herbicidas (DAA), até o desaparecimento dos sintomas. Para se proceder à análise anatômica das quatro espécies de gramas estudadas, o material foliar foi amostrado ao acaso na parcela, coletando-se as folhas inteiramente expandidas aos 7o, 21o e 35o dias após a aplicação dos herbicidas. Avaliou-se os caracteres quantitativos das regiões da nervura central (quilha) e da região compreendida entre a nervura central e a margem do limbo (asa). Os caracteres anatômicos foliares quantitativos analisados foram a porcentagem de epiderme das faces adaxial e abaxial, feixe vascular, bainha do feixe vascular, esclerênquima, parênquima, número e distância entre os feixes e espessura foliar. Avaliou-se também os caracteres morfológicos das gramas como a altura de planta, número e comprimento das inflorescências, número de estômatos e tricomas e massa...<br>The aim of this study was to evaluate the selectivity of the herbicides bentazon, nicosulfuron, halosulfuron, oxadiazon and 2,4-D applied in post-emergence on the Axonopus compressus (broadleaf carpetgrass), Paspalum notatum (bahiagrass), Stenotaphrum secundatum (St. Augustine grass) and Zoysia japonica (japanese lawn grass), and correlate the selectivity of these herbicides with the leaf anatomy of these species of grasses. The four species of grass had 48 months of age at the time of treatment application. The treatments were bentazon (720 g ha-1), nicosulfuron (50 g ha-1), halosulfuron (112.5 g ha-1), oxadiazon (875 g ha-1) and 2.4 D (698 g ha-1), and an untreated control for each species studied. The experimental design was randomized blocks design with four replications. Evaluations of phytotoxicity of species grasses were taken every seven days after herbicide application (DAA) until symptoms subside. To make the anatomical analysis of four species of grasses studied, the leaf material was sampled at random from the plot, collecting the expanded leaf leaves at 7, 21 and 35 days after herbicide application. It assessed the following quantitative traits regions of the midrib (keel) and the region between the midrib and the margin of the lamina (wing). The quantitative leaf anatomical characters were analyzed: the percentage of the adaxial epidermis and abaxial epidermis, vascular bundle, vascular bundle sheath, sclerenchyma, parenchyma, number and distance between the bundle vascular and leaf thickness. Evaluated the morphology of grasses such as plant height, number and length of inflorescences, number of stomata and trichomes and shoot dry matter. The results of phytotoxicity of herbicides on four species of... (Complete abstract click electronic access below)
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Marques, Renata Pereira 1984. "Seletividade de herbicidas aplicados sobre espécies de gramas em relação às características morfo-anatômicas foliares /." Botucatu :, 2012. http://hdl.handle.net/11449/99926.
Full textAbstract:
Orientador: Dagoberto Martins<br>Coorientador: Roberto Antonio Rodella<br>Banca: Carlos Alberto Mathias Azania<br>Banca: Pedro Luis da Costa Aguiar Alves<br>Banca: Mario Sergio Tomazela<br>Banca: Luciano Soares de Souza<br>Resumo: O objetivo desse trabalho foi avaliar a seletividade dos herbicidas bentazon, nicosulfuron, halosulfuron, oxadiazon e 2,4-D aplicados em pós-emergência sobre as espécies Axonopus compressus (grama São Carlos), Paspalum notatum (grama Batatais), Stenotaphrum secundatum (grama Santo Agostinho) e Zoysia japonica (grama Esmeralda) e relacionar a seletividade destes herbicidas com a anatomia foliar destas espécies de gramas. As quatro espécies de grama apresentavam 48 meses de idade no momento da aplicação dos tratamentos. Os tratamentos constituíram-se pela aplicação dos herbicidas bentazon (720 g ha-1), nicosulfuron (50 g ha-1), halosulfuron (112,5 g ha-1), oxadiazon (875 g ha-1) e 2,4-D (698 g ha-1), além de uma testemunha sem aplicação, para cada espécie avaliada. O delineamento experimental utilizado foi em blocos ao acaso com quatro repetições. As avaliações de fitotoxidade das espécies de gramas foram realizadas a cada 7 dias após a aplicação dos herbicidas (DAA), até o desaparecimento dos sintomas. Para se proceder à análise anatômica das quatro espécies de gramas estudadas, o material foliar foi amostrado ao acaso na parcela, coletando-se as folhas inteiramente expandidas aos 7o, 21o e 35o dias após a aplicação dos herbicidas. Avaliou-se os caracteres quantitativos das regiões da nervura central (quilha) e da região compreendida entre a nervura central e a margem do limbo (asa). Os caracteres anatômicos foliares quantitativos analisados foram a porcentagem de epiderme das faces adaxial e abaxial, feixe vascular, bainha do feixe vascular, esclerênquima, parênquima, número e distância entre os feixes e espessura foliar. Avaliou-se também os caracteres morfológicos das gramas como a altura de planta, número e comprimento das inflorescências, número de estômatos e tricomas e massa... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: The aim of this study was to evaluate the selectivity of the herbicides bentazon, nicosulfuron, halosulfuron, oxadiazon and 2,4-D applied in post-emergence on the Axonopus compressus (broadleaf carpetgrass), Paspalum notatum (bahiagrass), Stenotaphrum secundatum (St. Augustine grass) and Zoysia japonica (japanese lawn grass), and correlate the selectivity of these herbicides with the leaf anatomy of these species of grasses. The four species of grass had 48 months of age at the time of treatment application. The treatments were bentazon (720 g ha-1), nicosulfuron (50 g ha-1), halosulfuron (112.5 g ha-1), oxadiazon (875 g ha-1) and 2.4 D (698 g ha-1), and an untreated control for each species studied. The experimental design was randomized blocks design with four replications. Evaluations of phytotoxicity of species grasses were taken every seven days after herbicide application (DAA) until symptoms subside. To make the anatomical analysis of four species of grasses studied, the leaf material was sampled at random from the plot, collecting the expanded leaf leaves at 7, 21 and 35 days after herbicide application. It assessed the following quantitative traits regions of the midrib (keel) and the region between the midrib and the margin of the lamina (wing). The quantitative leaf anatomical characters were analyzed: the percentage of the adaxial epidermis and abaxial epidermis, vascular bundle, vascular bundle sheath, sclerenchyma, parenchyma, number and distance between the bundle vascular and leaf thickness. Evaluated the morphology of grasses such as plant height, number and length of inflorescences, number of stomata and trichomes and shoot dry matter. The results of phytotoxicity of herbicides on four species of... (Complete abstract click electronic access below)<br>Doutor
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Ackley, John A. "Efficacy and selectivity of the herbicide rimsulfuron in potatoes [Solanum tuberosum], transplanted tomatoes [Lycopersicum esculentum], and transplanted peppers [Capsicum annum]." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-06302009-040447/.
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Yenne, Samuel P. "Investigations on the mechanism of action of the oxime ether safeners for the protection of grain sorghum against metolachlor." Diss., Virginia Polytechnic Institute and State University, 1989. http://hdl.handle.net/10919/54821.
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Herbicide safeners (protectants, antidotes) are used to protect crop plants from herbicide injury. Currently our understanding of the mechanisms involved in the protection of plants by safeners is not well defined; therefore, investigations were conducted to elucidate the mechanism(s) of action of the oxime ether safeners. Molecular comparisons of selected herbicide-safener combinations using computer-aided molecular modeling revealed that the chemical structures of safeners and herbicides are very similar at the molecular level; and, indicate that these compounds could bind at the same active site of the target protein or they may serve as inducers of metabolic enzymes which detoxify herbicides. Metolachlor at 10 μM and seed-applied CGA-133205 had no effect on germination while treatment with seed-applied oxabetrinil significantly reduced germination of grain sorghum. Results from experiments on ¹⁴C-acetate incorporation into lipids indicate that metolachlor and the oxime ether safeners influence lipid metabolism causing a redistribution of carbon in the lipid fractions of germinating sorghum roots. Results from studies with acetyl-CoA carboxylase indicate that this enzyme is not a target site for either metolachlor or the oxime ether safeners. Metolachlor and the oxime ether safeners enhanced glutathione levels in grain sorghum seedlings at 12 to 48 hr after imbibition was initiated with oxabetrinil being more stimulatory than metolachlor or CGA-133205. Glutathione reductase activity was also stimulated in safener-treated grain sorghum seedlings. Both safeners slightly enhanced nonenzymatic and enzymatic conjugation of metolachlor with reduced glutathione. Oxabetrinil conjugated enzymatically or nonenzymatically with reduced glutathione at a slow rate, but CGA-133205 did not. These data suggest that during the early stages of seed germination and seedling development of grain sorghum, safeners can enhance the detoxication of metolachlor by enhancing glutathione levels and enzymatic and nonenzymatic conjugation of metolachlor with glutathione. It appears that oxabetrinil and CGA-133205 are conferring protection to grain sorghum by increasing the rate of metolachlor metabolism.<br>Ph. D.
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Lee, Codee Zebedee. "Postemergence Efficacy of Pyroxasulfone at Different Rates and Timings in Wheat." Thesis, North Dakota State University, 2018. https://hdl.handle.net/10365/29009.
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Pyroxasulfone is a VLCFA inhibitor labeled to control grasses and small-seeded broadleaf weeds. Little information is available regarding this herbicide being applied postemergence. Two field experiments were conducted to evaluate the efficacy of pyroxasulfone used postemergence. Pyroxasulfone applied to 2-leaf wheat controlled up to 83% of the green foxtail but had little to no effect on broadleaves. An additional field study was conducted to determine if pyroxasulfone could give supplemental green foxtail control when tank-mixed with ALS inhibitors. Few tank-mix combinations increased control, and the tank-mixes that did had inadequate control, <70%. Greenhouse experiments were also conducted. The first concluded that a wide range of grass species are susceptible to pyroxasulfone applied postemergence. The second demonstrated weed control with pyroxasulfone is almost exclusively due to root uptake. Contradicting levels of control between field and greenhouse experiments suggests more information is needed before pyroxasulfone can be utilized as a postemergence herbicide.<br>North Dakota State University. Department of Plant Sciences
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Cooley, William Edward. "Comparative studies on the modes of action of SC-0224 and glyphosate." Diss., Virginia Polytechnic Institute and State University, 1985. http://hdl.handle.net/10919/54493.
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The biological actions of the herbicides SC-0224 (trimethylsulfonium carboxymethylaminomethylphosphonate) and glyphosate [N-(phosphonomethyl)glycine] (PMG) were compared. In each study trimethylsulfonium iodide (TMS-I) was included as a treatment because the trimethylsulfonium ion is a constituent of the SC-0224 molecular structure.
In inflated duckweed (Lemna gibba L.), both formulated and technical grade forms of SC-0224 were found to be much more phytotoxic to duckweed than either formulated or technical grade forms of glyphosate. The growth inhibition caused by glyphosate was partially prevented by different combinations of the aromatic amino acids phenylalanine, tyrosine, and tryptophan; whereas, the duckweed growth inhibition caused by SC-0224 could not be reduced by the same amino acid combinations. TMS-I and SC-0224 were found to be equally phytotoxic to duckweed. SC-0224 caused larger increases than glyphosate in the pool levels of amino acids; the increases caused by SC-0224 were similar, however, to those caused by trimethylsulfonium iodide. Expressed on a per gram fresh weight basis none of the chemical treatments caused significant changes in soluble protein or the incorporation of ¹⁴C-leucine into soluble protein. On a per flask basis (allowing for decreased growth in treated flasks), both herbicides and TMS-I caused significant decreases in soluble protein and ¹⁴C-leucine incorporation. SC-0224 and TMS-I caused larger decreases than glyphosate in both cases but the SC-0224 and TMS-I treatments were not significantly different. These data indicate that differences in the phytotoxicity of SC-0224 .and glyphosate may be due to the action of the trimethylsulfonium ion of the SC-0224 structure.
The effects of these herbicides on the conversion of shikimate to anthranilate in a cell-free extract of Klebsiella pneumoniae ATCC 25306 were also compared. SC-0224 and glyphosate equally inhibited the production of anthranilate indicating that SC-0224 has action similar to glyphosate on the shikimate pathway.
The effects of these herbicides on photosynthetic electron transport (the Hill reaction) was determined using isolated thylakoids from Alaska pea (Pisum sativum L.). The action of SC-0224 was compared with the action of glyphosate, TMS-I and diuron [3-(3,4-dichorophenyl)-1,1-dimethylurea]. SC-0224, glyphosate and TMS-I did not inhibit the Hill reaction at concentrations up to 10 mM; whereas, diuron caused an almost total inhibition at 0.10 mM. The results of this study indicate that SC-0224 is not an inhibitor of photosynthetic electron transport.
These studies indicate that both constituents of the SC-0224 structure, TMS and PMG, are phytotoxic and may act independently.<br>Ph. D.
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Barberis, Luis Rodrigo Miyamoto [UNESP]. "Metodologia para determinação de efeitos fisiológicos e metabólicos do glufosinate em soja." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/99972.
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barberis_lrm_dr_botfca.pdf: 840865 bytes, checksum: 2158433b6e8b218bb15ad11592a60db9 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>O glufosinate é derivado do fosfinotricina, uma toxina microbiana natural isolada a partir de duas espécies de fungos Streptomyces. Atua inibindo a atividade da enzima glutamina sintetase, que é necessária para a produção do aminoácido glutamina e para a desintoxicação da amônia pela planta. Objetivou-se neste trabalho avaliar a intoxicação e alterações fisiológicas e bioquímicas causadas pelo glufosinate em plantas de soja. O experimento foi conduzido em casa-de-vegetação, no Núcleo de Pesquisas Avançadas em Matologia (Nupam), pertencente à Faculdade de Ciências Agronômicas – FCA/UNESP. Foi utilizado o cultivar de soja Nidera NS 7100 (RR) plantada em vasos de cinco de litros de solo. Foram realizados dois experimentos, sendo o primeiro experimento instalado com os seguintes tratamentos: duas formulações (glufosinate de amônio a 2,5 L p.c. ha-1 e glufosinate de potássio 2,5 L p.c. ha-1), três períodos de coleta de folhas dois, quatro e seis dias após aplicação e um tratamento testemunha sem aplicação. O segundo experimento foi instalado com os seguintes tratamentos: duas formulações (glufosinate de amônio e glufosinate de potássio), três doses de cada produto (0,625 L p.c. ha-1; 1,25 L p.c. ha-1; 2,5 L p.c. ha-1) e um tratamento testemunha sem aplicação. As variáveis analisadas em ambos os experimentos foram, amônia, compostos pertencentes à rota metabólica de ação do glufosinate (glutamato, glutamina, serina, ácido aminolevulênico, glufosinate), análise visual de fitotoxicicidade e ETR (taxa de transporte de elétrons). Para determinação de amônia foram desenvolvidas metodologias de extração para posterior quantificação em espectrofotômetro. Para determinação dos compostos, foram realizados...<br>The glufosinate is derived from phosphinothricin, a natural microbial toxin isolated from Streptomyces of two species of fungus. Acts inhibits the activity of glutamine synthetase, which is necessary for the production of the amino acid glutamine and for the detoxification of ammonia by the plant. The objective of this study was to evaluate the toxicity and physiological and biochemical changes caused by glufosinate in soybean plants. The experiment was carried out in green-house, in Faculty of Agronomic Sciences at São Paulo State University. We used soybean the cultivar NS 7100 Nidera planted in vases containing 5 liters of soil. Two experiments were carried out, the first experiment set with the following treatments: two formulations (glufosinate ammonium 2.5 L c.p. ha-1 and glufosinate potassium 4 2.5 L c.p. ha-1), three periods of leaf collection two, for and six days after application and a control without application. The second experiment was carried out with the following treatments: two formulations (glufosinate ammonium and glufosinate potassium), three doses of each product (0.625 L c.p. ha-1, 1.25 c.p. ha-1 L, 2.5 L c.p. ha -1) and a control without application. The variables analyzed in both experiments were ammonia, compounds belonging to the metabolic pathway of action of glufosinate (glutamate, glutamine, serine, aminolevulenic acid, glufosinate), visual analysis of plant injury and ETR (electron transport rate). To determine the ammonia were developed extraction methodologies for a quantification in spectrophotometric and for determination of compounds, extraction tests were performed to choose the most appropriate methodology, and development of analytical methods in LC-MS/MS (liquid chromatography and mass spectrometry). Plant injury evaluations were based on visual criteria and ETR measurements are given... (Complete abstract click electronic access below)
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Barberis, Luis Rodrigo Miyamoto 1975. "Metodologia para determinação de efeitos fisiológicos e metabólicos do glufosinate em soja /." Botucatu : [s.n.], 2012. http://hdl.handle.net/11449/99972.
Full textAbstract:
Orientador: Caio Antonio Carbonari<br>Coorientador: Edivaldo Domingues Velini<br>Banca: Fernando Tadeu de Carvalho<br>Banca: Marcus Barifouse Matallo<br>Banca: Cleber Daniel de Goes Maciel<br>Banca: Elza Alves Corrêa<br>Resumo: O glufosinate é derivado do fosfinotricina, uma toxina microbiana natural isolada a partir de duas espécies de fungos Streptomyces. Atua inibindo a atividade da enzima glutamina sintetase, que é necessária para a produção do aminoácido glutamina e para a desintoxicação da amônia pela planta. Objetivou-se neste trabalho avaliar a intoxicação e alterações fisiológicas e bioquímicas causadas pelo glufosinate em plantas de soja. O experimento foi conduzido em casa-de-vegetação, no Núcleo de Pesquisas Avançadas em Matologia (Nupam), pertencente à Faculdade de Ciências Agronômicas - FCA/UNESP. Foi utilizado o cultivar de soja Nidera NS 7100 (RR) plantada em vasos de cinco de litros de solo. Foram realizados dois experimentos, sendo o primeiro experimento instalado com os seguintes tratamentos: duas formulações (glufosinate de amônio a 2,5 L p.c. ha-1 e glufosinate de potássio 2,5 L p.c. ha-1), três períodos de coleta de folhas dois, quatro e seis dias após aplicação e um tratamento testemunha sem aplicação. O segundo experimento foi instalado com os seguintes tratamentos: duas formulações (glufosinate de amônio e glufosinate de potássio), três doses de cada produto (0,625 L p.c. ha-1; 1,25 L p.c. ha-1; 2,5 L p.c. ha-1) e um tratamento testemunha sem aplicação. As variáveis analisadas em ambos os experimentos foram, amônia, compostos pertencentes à rota metabólica de ação do glufosinate (glutamato, glutamina, serina, ácido aminolevulênico, glufosinate), análise visual de fitotoxicicidade e ETR (taxa de transporte de elétrons). Para determinação de amônia foram desenvolvidas metodologias de extração para posterior quantificação em espectrofotômetro. Para determinação dos compostos, foram realizados... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: The glufosinate is derived from phosphinothricin, a natural microbial toxin isolated from Streptomyces of two species of fungus. Acts inhibits the activity of glutamine synthetase, which is necessary for the production of the amino acid glutamine and for the detoxification of ammonia by the plant. The objective of this study was to evaluate the toxicity and physiological and biochemical changes caused by glufosinate in soybean plants. The experiment was carried out in green-house, in Faculty of Agronomic Sciences at São Paulo State University. We used soybean the cultivar NS 7100 Nidera planted in vases containing 5 liters of soil. Two experiments were carried out, the first experiment set with the following treatments: two formulations (glufosinate ammonium 2.5 L c.p. ha-1 and glufosinate potassium 4 2.5 L c.p. ha-1), three periods of leaf collection two, for and six days after application and a control without application. The second experiment was carried out with the following treatments: two formulations (glufosinate ammonium and glufosinate potassium), three doses of each product (0.625 L c.p. ha-1, 1.25 c.p. ha-1 L, 2.5 L c.p. ha -1) and a control without application. The variables analyzed in both experiments were ammonia, compounds belonging to the metabolic pathway of action of glufosinate (glutamate, glutamine, serine, aminolevulenic acid, glufosinate), visual analysis of plant injury and ETR (electron transport rate). To determine the ammonia were developed extraction methodologies for a quantification in spectrophotometric and for determination of compounds, extraction tests were performed to choose the most appropriate methodology, and development of analytical methods in LC-MS/MS (liquid chromatography and mass spectrometry). Plant injury evaluations were based on visual criteria and ETR measurements are given... (Complete abstract click electronic access below)<br>Doutor
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Moreira, Caio Alexandre Ferreira 1987. "Espectro de gotas e deriva de diferentes formulações dos herbicidas 2,4-D e Glyphosate aplicadas com pontas de jato plano com e sem indução de ar /." Botucatu, 2016. http://hdl.handle.net/11449/183163.
Full textAbstract:
Orientador: Ulisses Rocha Antuniassi<br>Banca: Otavio Jorge Grigoli Abi-Saab<br>Banca: Caio Antonio Carbonari<br>Banca: Paulo Roberto Arbex Silva<br>Banca: Rone Batista de Oliveira<br>Resumo: A correta aplicação de produtos fitossanitários resulta em minimização de perdas, e mitigação de danos ambientais. A busca por uma operação de aplicação otimizada tornou a mistura em tanque de produtos fitossanitários uma prática comumente utilizada em todo o mundo. Entretanto, a mistura em tanque pode resultar em alterações físicas na calda, interferindo no espectro de gotas e no risco de deriva. Técnicas para redução de deriva devem ser utilizadas neste processo, especialmente na aplicação de herbicidas. Nesse contexto, este trabalho teve como objetivo avaliar o espectro de gotas e o potencial de deriva gerado por caldas contendo os herbicida 2,4-D e glyphosate em diferentes condições de misturas, aplicadas com duas pontas diferentes. Em uma formulação composta de herbicida 2,4-D sal colina incluindo uma tecnologia para redução da deriva. Esta formulação foi comparada a uma formulação convencional de 2,4-D sal dimetilamina (DMA) de maneira isolada e em pré mistura com glyphosate (sal potássico e sal dimetilamina) e uma mistura pronta (2,4-D sal colina + glyphosate sal dimetilamina). Para isto foram comparados vinte tratamentos, sendo cada tratamento representado por uma calda, constituídas das misturas ou não entre os herbicidas. As avaliações dos tratamentos foram realizadas em dois experimentos. O primeiro pulverizado com uma ponta de jato plano de uso estendido (Teejet XR 11002) e outro com uma ponta jato plano de indução de ar (Teejet AIXR 11002). A pressão de trabalho ... (Resumo completo, clicar acesso eletrônico abaixo)<br>Abstract: The correct application of pesticides results in loss minimization, and mitigation of environmental damage. The search for an optimal implementation of operation has the mixture in a practical defensive tank commonly used throughout the world. However, the tank-mix can result in physical changes in the spray solution, interferes with droplet spectrum and the risk of drift. Drift reduction techniques to be used in this process, including the application of herbicides. In this context, this study aims to evaluate the spectrum of drops and the potential drift generated by spraying solutions containing the herbicide 2,4-D and glyphosate in different conditions of mixtures applied with two different nozzles. A formulation containing 2,4-D salt choline including a technology for reduction of drift was used. This formulation was compared to a conventional formulation of 2,4-D dimethylamine salt (DMA) in isolation and premix with glyphosate (potassium salt and dimethylamine salt) and a ready mixed (2,4-D + glyphosate salt choline salt dimethylamine). Twenty treatments were compared, each treatment being represented by a spray solution consisting of mixtures of herbicides or not. The treatments were evaluated in two experiments. A sprayed with a jet nozzle extended use plan (Teejet XR 11002) and one with a nozzle jet air induction plan (Teejet AIXR 11002) both at 280 kPa. The Volumetric Median Diameter (VMD) and the percentage of droplets smaller than 105 µm (V105) were obtained from ... (Complete abstract click electronic access below)<br>Doutor
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LaBar, Caitlin C. "Investigating the use of herbicides to control invasive grasses in prairie habitats effects on non-target butterflies /." Pullman, Wash. : Washington State University, 2009. http://www.dissertations.wsu.edu/Thesis/Spring2009/c_labar_042009.pdf.
Full textAbstract:
Thesis (M.S. in environmental science)--Washington State University, May 2009.<br>Title from PDF title page (viewed on May 29, 2009). "Program in Environmental Science and Regional Planning." Includes bibliographical references (p. 23-29).
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Macedo, Gabrielle de Castro [UNESP]. "Efeitos de sistemas de manejo pré-semeadura da soja sobre a dinâmica no solo e eficácia de herbicidas." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/126487.
Full textAbstract:
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000843127.pdf: 1278750 bytes, checksum: 44d10f457afba2e54aee4ae34fb8d5e2 (MD5)<br>A presença de palha na superfície do solo pode alterar a dinâmica populacional das plantas daninhas presentes na área, além de influenciar o comportamento dos herbicidas no solo. Atualmente, pouco se sabe a respeito da dinâmica de liberação de herbicidas com efeito residual quando sua aplicação é realizada sobre a cobertura verde, no manejo em pré-semeadura da soja, mas supõe-se que sua distribuição e dinâmica sejam alteradas. O presente trabalho teve por objetivos avaliar o comportamento no solo, a eficácia no controle de plantas daninhas e a seletividade para a cultura dos herbicidas sulfentrazone e flumioxazin quando aplicados sobre dois tipos de coberturas em diferentes sistemas de manejo de dessecação em pré-semeadura da soja. Realizaram-se dois experimentos, no município de Botucatu, São Paulo, em áreas com coberturas de milheto e sorgo, com delineamento em blocos casualizados, com 7 tratamentos e 4 repetições. Os tratamentos foram constituídos pela aplicação de herbicidas em diferentes sistemas de manejo das culturas de cobertura, sendo eles: glyphosate (720 g e.a. ha-1) 20 dias antes da semeadura (DAS) + sulfentrazone (600 g i.a. ha-1) 10 DAS; glyphosate (720 g e.a. ha-1) 20 DAS + flumioxazin (50 g i.a. ha-1) 10 DAS; glyphosate (720 g e.a. ha-1) + sulfentrazone (600 g i.a. ha-1) 10 DAS; glyphosate (720 g e.a. ha-1) + flumioxazin (50 g i.a. ha-1) 10 DAS; sulfentrazone (600 g i.a. ha-1) 10 DAS sem a presença de cobertura; flumioxazin (50 g i.a. ha-1) 10 DAS sem a presença de cobertura e uma testemunha com aplicação de glyphosate (720 g e.a. ha-1) 10 DAS. Foram realizadas avaliações da disponibilidade de ...<br>The presence of straw on the soil surface can alter the population dynamics of weeds present in the area, and influence the behavior of herbicides in soil. Currently, little is known about the dynamics of release of herbicides with residual effect when your application is performed on the green coverage in the management of pre-sowing soybean, but it is assumed that the distribution and dynamics are altered. This study aimed to evaluate the behavior in soil, the efficiency in weed control and selectivity to the culture of sulfentrazone and flumioxazin herbicide when applied over two types of covers in different desiccation management systems in pre-sowing soybean. There were two experiments, in Botucatu, São Paulo, in areas with millet and sorghum coverage, in a randomized block design with 7 treatments and 4 replications. The treatments were a combination of herbicides in different management systems of coverage crops, which are: glyphosate (720 g e.a. ha-1) 20 days before sowing (DBS) + sulfentrazone (600 g i.a. ha-1) 10 DBS; glyphosate (720 g e.a. ha-1) 20 DBS + flumioxazin (50 g i.a. ha-1) 10 DBS; glyphosate (720 g e.a. ha-1) + sulfentrazone (600 g i.a. ha-1) 10 DBS; glyphosate (720 g e.a. ha-1) + flumioxazin (50 g i.a. ha-1) 10 DBS; sulfentrazone (600 g i.a. ha-1) 10 DBS without the presence of coverage; flumioxazin (50 g i.a. ha-1) 10 DBS without the presence of cover and a control with application of glyphosate (720 g e.a. ha-1) 10 DBS. Were evaluated the availability of herbicides in the soil through periodic collections, weed control, weed survey, selectivity of herbicides to the crop, production and soybean yield components. For quantification of herbicides used a LCMS-MS system consisting of a High Performance Liquid Chromatography (HPLC) coupled with mass spectrometry. For flumioxazin, amounts of herbicide present in the samples were below the detection limit of the analytical method ...
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Pazuch, Daiana. "Tolerância ao glyphosate e sua absorção e translocação por biótipos de Ipomoea spp." Universidade Tecnológica Federal do Paraná, 2013. http://repositorio.utfpr.edu.br/jspui/handle/1/676.
Full textAbstract:
As espécies de Ipomoea estão entre as mais tolerantes ao glyphosate. A forte
pressão de seleção exercida pelo uso intensivo desse herbicida em áreas cultivadas
favorece o surgimento de espécies com alto grau de tolerância a esse produto.
Conhecer as espécies mais comuns em áreas cultivadas, os métodos de superação
de dormência adequados para cada espécie e os seus mecanismos de tolerância ao
glyphosate é fundamental para o estabelecimento de estratégias de prevenção e
manejo. O segundo capítulo da dissertação tratou da seleção de métodos eficazes
de superação de dormência nas espécies Ipomoea indivisa, I. grandifolia e I.
purpurea e na determinação de como os mesmos influenciam a cinética da germinação das sementes dessas espécies. Os tratamentos foram água quente, escarificação mecânica, escarificação mecânica + resfriamento, escarificação química e testemunha. Foram avaliados a porcentagem de germinação, tempo
médio, índice de velocidade e frequência relativa de germinação. No terceiro
capítulo foram identificadas as espécies de Ipomoea mais comuns em lavouras de
soja da região Sudoeste do Paraná e Oeste de Santa Catarina, através de dois
experimentos de resposta a doses de glyphosate. No experimento preliminar, foram
definidas doses de glyphosate a serem empregadas no ensaio subsequente,
empregando-se um biótipo de cada uma das espécies I. grandifolia, I. indivisa e I.
purpurea. Nos experimentos subsequentes, em 31 biótipos (20 de I. grandifolia, 7
de I. indivisa e 4 de I. purpurea) foram aplicadas 0, 216, 432, 864, 1.296, 1.728 e
2.160 g ha-1 de glyphosate (doses em e. a.) em plantas com 6 a 8 folhas
verdadeiras. O desempenho do herbicida foi avaliado através do controle aos 7, 14
21 e 28 dias após a aplicação (DAA), da massa da parte aérea verde (MPAV) e seca
(MPAS) aos 28 DAA. O quarto capítulo visou identificar se a absorção foliar e a
translocação são mecanismos determinantes da tolerância ao glyphosate em
espécies e biótipos de Ipomoea. Foram avaliados um biótipo tolerante (T) e um
sensível (S) das espécies I. grandifolia, I. indivisa e I. purpurea. Quando essas
apresentavam entre 3 e 4 folhas expandidas foi aplicado 360 g ha-1 de 14Cglyphosate+
padrão analítico. Foram analisadas a absorção foliar e a translocação 2,
4, 8, 12, 24, 48 e 72 horas após a aplicação (HAA) do herbicida. A escarificação com
ácido sulfúrico foi o método de superação de dormência mais indicado para I.
indivisa e I. purpurea, e o tratamento com água quente foi o mais eficiente para I.
grandifolia. As espécies de Ipomoea mais comuns no levantamento foram I. indivisa,
I. purpurea e I. grandifolia. Ocorre grande variabilidade de tolerância ao glyphosate
entre biótipos de uma mesma espécie e entre espécies. Alguns biótipos necessitam
dose muito superior à recomendada para a espécie para serem controlados
satisfatoriamente. A tolerância ao glyphosate em biótipos de Ipomoea grandifolia
está associada à absorção e translocação reduzida nas plantas, e em biótipos de I.
indivisa e I. purpurea à reduzida translocação de glyphosate.<br>The Ipomoea species are among the most tolerant to glyphosate. The strong
selection pressure exerted by the intensive use of this herbicide in cultivated areas
favors the emergence of species with high tolerance. Knowing the most common
species in cultivated areas, appropriate methods of dormancy overcoming for each
species and their mechanisms of tolerance to glyphosate is crucial for the
establishment of strategies for prevention and management. The second chapter of
the dissertation dealt with the selection of effective methods of overcoming dormancy
in Ipomoea species I. indivisa, I. grandifolia and I. purpurea and determining how
they affect the germination kinetics of these species. Treatments were hot water,
chiseling, chiseling + cooling, chemical scarification and a check. We evaluated
germinability, mean time, speed index and relative frequency of germination. In the
third chapter we identified the most common species of Ipomoea in soybean crop
from southwestern Paraná and west of Santa Catarina, through two experiments of
response to rates of glyphosate. In preliminary experiments were defined glyphosate
rates to be used in a subsequent assay, using one biotype of the species I.
grandifolia, I. indivisa and I. purpurea. In the subsequent experiments, in 31 biotypes
(20 I. grandifolia, 7 I. indivisa and 4 I. purpurea) were sprayed 0, 216, 432, 864,
1,296, 1,728 and 2,160 g ha-1 of glyphosate in plants with 6-8 true leaves. The
performance of the herbicide was evaluated using the control at 7, 14 21 and 28 days
after application (DAA), the mass of fresh (MPAV) and dry (SDW) shoots at 28 DAA.
The fourth chapter aimed to identify whether foliar absorption and translocation
mechanisms are determinants to tolerance of Ipomoea species and biotypes to
glyphosate. Were compared one biotype tolerant (T) and one sensitive (S) of species
I. grandifolia, I. indivisa and I. purpurea. When the plants had between 3 and 4
expanded leaves was applied 360 g ha-1 of 14C-glyphosate + analytical standard. We
analyzed the foliar uptake and translocation 2, 4, 8, 12, 24, 48 and 72 hours after
application (HAA) of the herbicide. The most common species of Ipomoea in the
survey were I. indivisa, I. purpurea and I. grandifolia. Scarification with sulfuric acid
was the best method of overcoming dormancy for I. indivisa and I. purpurea, and the
hot water treatment was most effective in I. grandifolia. There was great variability of
tolerance to glyphosate between biotypes of the same species and between species.
Some biotypes required much more than the recommended dose for the species to
be controlled satisfactorily. The tolerance to glyphosate in I. grandifolia biotypes was
associated with reduced absorption and translocation in plants, and in I. Indivisa
biotypes and I. purpurea with reduced translocation of glyphosate.
50
Macedo, Gabrielle de Castro. "Efeitos de sistemas de manejo pré-semeadura da soja sobre a dinâmica no solo e eficácia de herbicidas /." Botucatu, 2015. http://hdl.handle.net/11449/126487.
Full textAbstract:
Orientador: Caio Antonio Carbonari<br>Banca: Edivaldo Domingues Velini<br>Banca: Anderson Luis Cavenaghi<br>Resumo: A presença de palha na superfície do solo pode alterar a dinâmica populacional das plantas daninhas presentes na área, além de influenciar o comportamento dos herbicidas no solo. Atualmente, pouco se sabe a respeito da dinâmica de liberação de herbicidas com efeito residual quando sua aplicação é realizada sobre a cobertura verde, no manejo em pré-semeadura da soja, mas supõe-se que sua distribuição e dinâmica sejam alteradas. O presente trabalho teve por objetivos avaliar o comportamento no solo, a eficácia no controle de plantas daninhas e a seletividade para a cultura dos herbicidas sulfentrazone e flumioxazin quando aplicados sobre dois tipos de coberturas em diferentes sistemas de manejo de dessecação em pré-semeadura da soja. Realizaram-se dois experimentos, no município de Botucatu, São Paulo, em áreas com coberturas de milheto e sorgo, com delineamento em blocos casualizados, com 7 tratamentos e 4 repetições. Os tratamentos foram constituídos pela aplicação de herbicidas em diferentes sistemas de manejo das culturas de cobertura, sendo eles: glyphosate (720 g e.a. ha-1) 20 dias antes da semeadura (DAS) + sulfentrazone (600 g i.a. ha-1) 10 DAS; glyphosate (720 g e.a. ha-1) 20 DAS + flumioxazin (50 g i.a. ha-1) 10 DAS; glyphosate (720 g e.a. ha-1) + sulfentrazone (600 g i.a. ha-1) 10 DAS; glyphosate (720 g e.a. ha-1) + flumioxazin (50 g i.a. ha-1) 10 DAS; sulfentrazone (600 g i.a. ha-1) 10 DAS sem a presença de cobertura; flumioxazin (50 g i.a. ha-1) 10 DAS sem a presença de cobertura e uma testemunha com aplicação de glyphosate (720 g e.a. ha-1) 10 DAS. Foram realizadas avaliações da disponibilidade de ...<br>Abstract: The presence of straw on the soil surface can alter the population dynamics of weeds present in the area, and influence the behavior of herbicides in soil. Currently, little is known about the dynamics of release of herbicides with residual effect when your application is performed on the green coverage in the management of pre-sowing soybean, but it is assumed that the distribution and dynamics are altered. This study aimed to evaluate the behavior in soil, the efficiency in weed control and selectivity to the culture of sulfentrazone and flumioxazin herbicide when applied over two types of covers in different desiccation management systems in pre-sowing soybean. There were two experiments, in Botucatu, São Paulo, in areas with millet and sorghum coverage, in a randomized block design with 7 treatments and 4 replications. The treatments were a combination of herbicides in different management systems of coverage crops, which are: glyphosate (720 g e.a. ha-1) 20 days before sowing (DBS) + sulfentrazone (600 g i.a. ha-1) 10 DBS; glyphosate (720 g e.a. ha-1) 20 DBS + flumioxazin (50 g i.a. ha-1) 10 DBS; glyphosate (720 g e.a. ha-1) + sulfentrazone (600 g i.a. ha-1) 10 DBS; glyphosate (720 g e.a. ha-1) + flumioxazin (50 g i.a. ha-1) 10 DBS; sulfentrazone (600 g i.a. ha-1) 10 DBS without the presence of coverage; flumioxazin (50 g i.a. ha-1) 10 DBS without the presence of cover and a control with application of glyphosate (720 g e.a. ha-1) 10 DBS. Were evaluated the availability of herbicides in the soil through periodic collections, weed control, weed survey, selectivity of herbicides to the crop, production and soybean yield components. For quantification of herbicides used a LCMS-MS system consisting of a High Performance Liquid Chromatography (HPLC) coupled with mass spectrometry. For flumioxazin, amounts of herbicide present in the samples were below the detection limit of the analytical method ...<br>Mestre