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1
Shilubane, Paul Xilavi. "The ethnobotany of the Tsonga-Shangana in selected areas of Bushbuckridge in the Bohlabela District of the Limpopo Province." Thesis, University of Limpopo, 2008. http://hdl.handle.net/10386/2397.
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Thesis (PhD. (Anthropology)) --University of Limpopo, 2008In this study, the multidisciplinary epistemology of ethnobotany is reviewed within the wider context of ethnoecology and indigenous knowledge systems. The major problems derived from this theoretical framework cover aspects of the relationship between cultural groups and their natural environment - particularly the botanical component of the latter. Problems and critical questions about this relationship were investigated in selected areas of the Bushbuckridge district, which are inhabited by a number ofTsonga-Shangana communities. Salient aspects of the natural habitat in the study area are discussed with special reference to the vegetation, climate and soil types. A proper understanding of the culturally-based exploitation of plant resources necessitates a sound knowledge of the culture concerned, including relevan t aspects of its value-system and cosmology. In this review, emphasis is placed on those aspects of culture that are directly concerned with the utilisation of plant resources. The latter include a wide range of economic, technological and medicinal uses. Specimens of 200 plants were collected and identified by the University of Limpopo Herbarium. The uses of every species are described. Systematised tables of plant uses are presented, as well as comparative lists in which selected uses are compared to those of other cultures in which the same species are used. The effects of plant utilisation, as well as indigenous forms of resource management, are also discussed. Recommendations are made on how to counter the process of environmental degradation which is caused by over-exploitation .
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Mathabe, Matlakala Christina. "Bioactivity of medicinal plants used for treatment of diarrhoea in selected villages in Limpopo Province, South Africa." Thesis, University of Limpopo (Turfloop Campus), 2006. http://hdl.handle.net/10386/925.
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Khosa, Josephine. "The legal protection of indigenous plants with reference to pterocarpus angolensis in Rolle village, Limpopo Province." Thesis, University of Limpopo (Turfloop Campus), 2008. http://hdl.handle.net/10386/825.
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Boloko, Mahlodi Solomon. "The influence of forage legumes on annual fodder grasses in different intercropping systems in the Limpopo Province." Thesis, University of Limpopo (Turfloop Campus), 2004. http://hdl.handle.net/10386/884.
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Thesis (M.Sc. (Agricultural Management)) --University of Limpopo, 2004Identification of annual grass/legume intercropping or mixtures with superior nutrient traits and Dry matter (DM) production is critical to increasing productivity of the crop and animal production among small-scale farmers in the Limpopo Province. Three similar field experiments were established at different locations in the Province to determine the significance of the contribution of annual summer legumes, and cutting treatments on the nutritive value and dry matter accumulation of the popular forage sorghum (Sorghum spp) and pearl millet (Pennisetum glaucum) intercropped with cowpea (Vigna unguiculata) and dolichos (Lablab purpureus). The cropping systems evaluated were sole sorghum, sole pearl millet, sorghum + cowpea, sorghum + dolichos, pearl millet + cowpea and pearl millet + dolichos. The treatments sole sorghum and pearl millet significantly (P<0.05) outperformed the other treatments in terms of DM production at most cutting stages. The remaining four treatments though, inferior in DM in this study, yielded better than the average yield on farmers' fields in the Province. Higher protein content was obtained in mixtures than in sole cropping, and generally there was lower protein production and content at matured stages (CT3) in the study. The other chemical composition analyzed in the study was not significant for both mixtures and sole cultures.
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Masekoameng, Mosima. "Indigenous knowledge systems in food gathering and production in selected rural communities in Sekhukhune District of the Limpopo Province." Thesis, University of Limpopo, 2007. http://hdl.handle.net/10386/1836.
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Obi, CL, MNB Momba, A. Samie, JO Igumbor, E. Green, and E. Musie. "Microbiological, physico-chemical and management parameters impinging on the efficiency of small water treatment plants in the Limpopo and Mpumalanga Provinces of South Africa." Water SA, 2007. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1000861.
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In the wake of the growing dependence on small water treatment plants (SWTPs) in providing quality water to rural areas and the global burden of water borne diseases, this study sought to examine the efficiency of 55 SWTPs located in rural or peri-urban areas of Limpopo and Mpumalanga Provinces in order to gauge the safety of water supply for human consumption. The microbiological and physical parameters of raw water, treated water and water in the distribution systems were examined using standard methods. Management issues impacting on quality of water supply were determined by use of questionnaires and focus group discussions. Results obtained showed that the pH, turbidity, temperature and conductivity of the raw water in SWTPs studied in both provinces ranged between 6.46 to 9.05 pH units, 0.19 to 8.0 NTU, 15.4oC to 31.40oC and 44.40.4 μS to 108 μS respectively. Water quality compliance at point of use (treated water) according to the Department of Water Affairs and Forestry of South Africa guidelines in SWTPs studied in both provinces were 85% for faecal coliforms and 69% for total coliforms. In the distribution systems, TCCs, FCCs and HPCs were within recommended limits except for few SWTPs suggesting a possibility of inadequate treatment and this may represent post-treatment contamination and possible risk of infection from these water supply sources. Physical parameters were generally within the recommended ranges . In terms of administrative issues, some plant operators did not have adequate knowledge of the functioning of the SWTPs and most were unable to calculate chlorine dosage, determine flow rates or undertake repairs of basic equipment. Poor working conditions , frequent stock depletion of chemicals , lack of maintenance culture , lack of emergency preparedness and poor communication were also cited .
The study has revealed that the microbiological quality of raw water was very poor but that water treatment was efficient in the majority of SWTPs studied in both provinces. Regular monitoring of microbial and physico-chemical parameters of water quality served by the different SWTPs to the population is recommended to gauge their safety for human consumption. Issues such as enhanced incentives and periodic training of plant operators, improved communication and conditions of service , periodic stock inventory and entrenchment of maintenance culture may be necessary to ensure sustained and efficient water distribution systems.
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Sithavhakhomu, Thilivhali Simon. "Challenges facing local communities in utilising and sustaining indigenous medicinal plants in the Thengwe village of Limpopo Province." Thesis, University of Limpopo (Turfloop Campus), 2012. http://hdl.handle.net/10386/827.
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Thesis (M.Dev.) --University of Limpopo, 2012The main aim or purpose of this study was to look at the ways and the strategies of minimizing\ mitigating the over-harvesting of indigenous medicinal plants in order to use them sustainably. The task of identifying and assessing challenges facing local communities in utilizing and sustaining indigenous medicinal plants in Thengwe village and their implications on environmental conservation and management of natural resources was done during the pilot survey. The environmental field survey was conducted after gaining permission to enter into the identified study areas including the Mutavhatsindi Nature Reserve. The result revealed that most of the indigenous medicinal plants are used for healing, religious, economical and for protection purposes. Most of the indigenous medicinal plants in the study area are depleted and many of them are facing extinction. The study was qualitative in design. Semi-structured interviews and a questionnaire were used. The questionnaire had both open-ended and closed questions. The study area was Thengwe Village in the Mutale Municipality. The existence of medicinal plants is threatened by people `s perceptions and attitudes towards them. For some indigenous medicinal plants, depletion is at a high rate due to over-harvesting and because the plants when traded by herbalists they have a premium price attached to them. Interestingly, perhaps surprisingly, the majority of young people hold negative attitudes towards the indigenous medicinal plants due to poor knowledge of the value of indigenous medicinal plants, in contrast to the elders who appreciate their role and consider them as part of their culture, economy and religion. One of the findings is that medicinal plants were found to be important to the community members as the source of income and creation of jobs for the medicinal collectors who sell the species to traditional healers. For the reason of preserving indigenous medicinal plants and others as the environmental resources, this study recommends that there should be strong intergovernmental relationships between the National, Provincial and Local governments in order to prevent over-harvesting of the medicinal plants. The indigenous medicinal plants are equally important to biodiversity students and researchers who want to study and research indigenous medicinal plants which play an important role in the improvement of the livelihoods of community members. Furthermore, education campaigns within the communities and school learners are recommended to encourage the prevention, sustainability and utilization of the indigenous medicinal plants. Additionally, the legal authorities should be empowered to prosecute all people who may be found illegally in possession of indigenous medicinal plants, as well as endangered and protected species. Heavy fines and charges should be imposed on such culprits. Of importance is the fact that the results and recommendations of this study may facilitate the teaching of environmental education and management of natural resources as well as boost the local economy of the Vhembe district Municipality by showing that medicinal plants in the area can be seen as a viable tourist attraction.
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Nchabeleng, Lehlohonolo. "Effects of different climatic and soil factors at different locations on chemical composition of bush tea (Athrixia phylicoides DC.)." Thesis, University of Limpopo (Turfloop Campus), 2012. http://hdl.handle.net/10386/740.
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Lebogo, Kgomotso Welheminah. "The evaluation of the effects of semi-purified extracts of Commelina benghalensis on the molecular events associated with the growth, apoptosis and cell cycle progression of Jurkat-T cells." Thesis, University of Limpopo (Turfloop Campus), 2007. http://hdl.handle.net/10386/914.
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Rasekgala, Mokgadi Thelma. "The ethno-ecological assessment of Cassia abbreviata Oliv. at Matsa village, Limpopo province, South Africa." Diss., 2018. http://hdl.handle.net/11602/1090.
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MSc (Botany)Department of Botany
Since time immemorial plants have traditionally been used for various purposes in many parts of the world including Vhembe district, Limpopo province of South Africa. The use of C. abbreviata plants at Matsa village of Vhembe district also contributes significantly to the livelihood of the community. However, little work has been made in the past to properly document and promote the knowledge. The purpose of the present study was to record and analyse the ethnobotanical knowledge of C. abbreviata within the people of Matsa village around Nzhelele in Vhembe district. Semi-structured questionnaires were administered on 50 informants from different households selected randomly to gather data regarding the local name of C. abbreviata, the uses and its availability. Significantly higher number of medicinal use was reported by elderly people including both men and women as compared to middle-aged, younger people. Other use categories included firewood, furniture and joinery production and shade for people in the area. Awareness on the importance of the species should therefore be created amongst the people, especially the young ones. Understanding the ethnobotanical knowledge of indigenous species within an area is crucial towards development of its management plan.
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Mboweni, Hlayisa Fredah. "Antimicrobial, cytotoxic and prelimenary phytochemical analysis of four medicinal plants and their formulation." Diss., 2018. http://hdl.handle.net/11602/1096.
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MSc (Microbiology)Department of Microbiology
BACKGROUND: Medicinal plants form an important part of the Southern African cultural heritage. Indigenous populations, for example the Vha-Venda people, tend to use medicinal plants in formulations rather than western medicines for health and survival. In order to certify and give scientific credibility to the use of medicinal plants formulations used by Vha-Venda people for the treatment of diseases, several assays were carried out. The present study was aimed at assessing phytochemical content, antimicrobial, antioxidant and cytotoxic activities of four indigenous Venda medicinal plants in a formulation and compare their activity with each plant used individually. METHODS: Peltophorum africanum (roots), Pterocarpus angolensis (bark), Terminalia sericea (roots) and Ximenia caffra (roots) were collected from the Thohoyandou area. The collected plant parts were extracted with methanol and water respectively. Individual plant extracts and Five designed formulations were tested for their antimicrobial activity against Staphylococcus aureus ATCC 25923 (Methicillin Resistant), Staphylococcus aureus ATCC 33591(Methicillin Susceptible), beta lactamase producing Klebsiella pneumonia (ATCC 700603) and extended spectrum beta lactamase producing E. coli (ATCC 35218), four clinical isolates of Candida spp and Cryptococcus neoformans using the Broth dilution method. Minimum bactericidal concentration (MBC) of the extracts was determined by culturing the contents of minimum inhibitory concentration (MIC) on nutrient agar. Similarly, minimum fungicidal concentration (MFC) was also determined by culturing contents of MIC in sabouraud dextrose agar (SDA). Extracts were further assessed for their total phenolic content, total flavonoid content and Qualitative phytochemical analysis. The antioxidant ability of the plants extracts and formulations to scavenge free radical DPPH was also determined. The plant formulations were assessed for their anti-HIV activity using the reverse transcriptase colorimetric assay kit. Cytotoxicity against human lymphatic endothelial cells (HLEC) was determined using MTT assay. RESULTS: Methanolic and aqueous extracts of T. sericea exhibited the best antifungal and antibacterial activities whilst P. angolensis and X. caffra showed poor activities. Methanolic plant formulations showed good activities compared to aqueous formulations. However, Fractional Inhibition Concentration Index showed that there was 1 synergistic interaction, 25 additive interactions and 14 antagonistic interactions between the plant extracts. The methanolic formulation 3 showed the best overall phenolic content at 11.85±0.109 mgGAE/g whilst aqueous X. caffra extract showed the least content at 4.546±0.104 mgGAE/g. Higher total flavonoid contents were seen in methanolic formulation 4 at 2.75±0.02 mgQE/g. Qualitative phytochemical analysis revealed the presence of flavonoids, phenolics, terpenoids, tannins, saponins and steroids in 80% of the tested plant extracts and formulations. All plant extracts and formulations exhibited good antioxidant activity against DPPH. The methanolic formulation showed the best antioxidant activity with IC50 of 0.094 ± 0.33μg/ml. For anti- HIV inhibition, all formulations at 200μg/ml exhibited higher percentage of HIV-1 reverse transcriptase inhibition with methanolic mixture 3 being the best overall at 97.5% activity whilst aqueous mixture5 was the least active with 63.03% inhibition activity. Moreover, the best anti-HIV activity at 100μg/ml was exhibited by methanolic mixture 3 at 71% inhibition. Furthermore, aqueous X. caffra, mixture 2 inhibited 26% and 51% at 12.5mg/ml and 3.125mg/ml respectively. Peltophorum africanum and mixture 5 inhibited 34%, 54% and 43% at 3.125mg/ml, 6.25mg/ml and 12.5 mg/ml respectively of Human Lymphatic Endothelial cells growth. CONCLUSIONS: The results from the study indicated that most of the commonly used traditional medicinal Plants in the Venda region when mixed together have merit for use in traditional medical practice as they have shown good antimicrobial activities, good antioxidant xviii activities, good phytochemical activities and good cell proliferation activity. However some formulations showed antagonistic interaction against bacteria. Some Individual medicinal plants showed toxicity at higher concentrations against immune cells. Whereas formulations promoted cell proliferation, therefore, the use of such individual plants in the treatment of infections should be highly monitored as they may pose a health threat to normal immune cells. Generally, plants are potential pharmacological agents which needs to be preserved and harvested with care.
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Ojelade, Babatube Solomon. "Regeneration potential of selected medicinal plants used to treat human and livestock diseases in Limpopo Province of South Africa." Diss., 2017. http://hdl.handle.net/11602/1069.
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MSCAGR ( Plant Production)Many high valued tree species of medicinal significance in the Limpopo Province, South Africa exhibit seed dormancy, and also contain aromatic oils which inhibit rooting of their stem cuttings. These plant species are under pressure due to human over-exploitation. The main objective of this study was to investigate effects of rooting hormones on the rooting ability that will help in domesticating some of the selected high valued medicinal plants, Elaeodendron transvaalense (bushveld saffron), Brackenridgea zanguebarica (yellow peeling plane), and Warburgia salutaris (pepper-bark tree). Stem cuttings of these plant species were prepared and treated with various concentrations (500, 1000 and 2000 ppm) of IBA, IAA and NAA in different growth media (Natural soil, farm soil and hygromix) at a nursery house. 180 experimental units were sown and arranged in Randomized Complete Block Design (RCBD), each treatment replicated five times and then monitored for a period of three months. Data were only obtained from Brackenridgea zanguebarica as other species dried up two weeks after sprouting. The two variables measured from the experiments were sprouted stems and number of leaves. The data obtained were subjected to analysis of variance and least significant difference (LSD) at 5% probability level was used to compare treatment using STATISTICA software analysis package. The hormone, hormone concentration, growth media and their interactions had effect on sprouted stems and number of leaves produced on Brackenridgea zanguebarica cuttings, with no record of rooting ability. IBA (500 ppm and 1000 ppm) and control (without rooting hormone) showed high significant results with natural soil and farm soil in terms of leaf production compared to the hygromix, which is significantly lower from others. IBA at the various concentrations (500, 1000 and 2000 ppm) and the control gave the highest percentage sprouted stem on both natural soil and farm soil as compared with other hormones at the same
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Tiawoun, Makuete Andre. "Conservation Strategies of the Red Listed Brackenridgea zanguebarica Oliv. in Vhembe District Municipality, Limpopo Province, South Africa." Thesis, 2019. http://hdl.handle.net/11602/1471.
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PhD (Botany)Department of Botany
Brackenridgea zanguebarica Oliv. (Ochnaceae) commonly known as “Yellow Peeling Plane”, is an important medicinal plant species, and one of the most threatened tree species that is endemic to Thengwe village in Vhembe District, South Africa. It is widely harvested for traditional purposes and difficult to propagate sexually and asexually. Nowadays, it has become threatened and this unique plant population is believed to be declining in the Brackenridgea Nature Reserve (BNR). According to the Red List of South African plants, B. zanguebarica is considered a critically endangered medicinal plant. All these facts combined with a lack of knowledge on the conservation of this species prompted this study on its conservation. To ascertain the effective conservation and proper management of this multipurpose tree, this study was conducted with the aim to improve its conservation strategy by investigating current threats to the existence of B. zangueberica, the chemical constituents from the different plant parts, the propagation methods of the species, and the factors delaying the germination of its seeds. In this study, an assessment of the current population threats was important as a first step in conservation; this would update the status of the species in its natural habitat. The current status of Brackenridgea zanguebarica was conducted through an extensive field survey in the Brackenridgea Nature Reserve (BNR), where a belt transect method was deployed to record quantitative information, while the harvesting damage was estimated using a sliding scale from 0 to 5. The structure of the populations in terms of the stem diameter size classes showed a bell shape vi pattern. About 60% of individuals showed some signs of plant parts extraction as the stem bark was reported to be the main plant part harvested. The population structure, however, was dominated by juvenile plants due to the selective harvesting of mature individuals; this hinders fruit production leading to poor or slow seed production. The present study aimed to compare the chemical constituents and the antimicrobial activities of some parts of this plant species. The extracts were screened for phytochemicals using standard methods. Thin-layer chromatography (TLC) was carried out to compare the chemical constituents using various solvent systems of varying polarity. Agar well diffusion and broth microdilution methods were used to evaluate the antimicrobial activity and the minimum inhibitory concentrations (MIC) of plant extracts respectively, against three bacteria and four fungi. The phytochemical analysis revealed a close similarity of various phytoconstituents of the three plant parts (stem bark, twigs and leaves). TLC analysis showed a slight difference in the acetone extract of different plant parts with more bands on the leaves than the stem bark and twigs. Acetone extracts of the three plant parts exhibited varying degrees of antimicrobial activity against Staphylococcus aureus and Candida glabrata, while none of the extracts showed any activity against Escherichia coli, Klebsiella pneumonia, Candida albicans, C. krusei, and C. parapsilosis. However, analysis of the antibacterial activity of various extracts revealed that the best inhibitory activity was produced by the stem bark extract compared to the leaf and twig extract. vii The sustainable way to meet the growing demand of Brackenridgea zanguebarica is to increase its availability through propagation practices. Unfortunately, the conservation of B. zanguebarica is hampered by lack of information on how it can be propagated. Sexual and asexual propagations were conducted to determine an efficient method to increase the number of individuals of this plant species. Seed propagation was carried out to study the effect of various pre-treatments on germination, in order to understand the germination requirements; vegetative propagation was conducted to assess if B. zanguebarica could be successfully propagated via stem cuttings if the appropriate growth hormones and growing media were applied. Brackenridgea zanguebarica seed did not germinate at all under any of the conditions tested. However, the results, showed the potential of propagating this species from stem cutting despite the poor results obtained. Thus B. zanguebarica was found to be difficult to propagate sexually and asexually. Seeds serve as a means of reproduction and a vital element to ensure the survival of plant species. Seed germination is controlled by a number of factors. The propagation of B. zanguebarica via seed is very difficult and research has not been undertaken to understand possible factors that may delay its germination. This study was aimed to investigate and describe both the morphological and anatomical features of B. zanguebarica seed, in order to identify structural features implicated in its poor germination. To achieve this goal, a morphological and anatomical study was conducted based on the observation of seeds under a scanning electron microscope (SEM) and light microscopy (LM). Water uptake potential was assessed by the increase in seed mass. The morpho-anatomical analysis indicated that some structures of the seed, such as seed coat, and the presence of endosperm viii surrounding the embryo that consisted of two prominent cotyledons, seem to greatly hinder the germination of this species. The research findings provided information regarding the seed structure which is one important step in identifying useful techniques to improve seed germination for conservation purposes.
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Magoro, Madimetja David. "Traditional health practitioners' practices and the sustainability of extinction-prone traditional medicinal plants." Diss., 2008. http://hdl.handle.net/10500/2292.
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For centuries Traditional Health Practitioners (THPs) used their indigenous knowledge (IK) in conserving medicinal plants and environments to maintain sustainability. With the rapid environmental, social, economic and political changes occurring in many areas inhabited by rural people exist the danger that the loss of biodiversity from habitat destruction and unsustainable harvesting practices will result in some species becoming extinct.
The main aim of the study was to determine the natural habitat of extinction-prone traditional medicinal plants combining the insight of THPs with an ultimate goal of guiding research for the conservation, propagation and cultivation of traditional medicinal plants. Despite problems, opportunities and challenges expressed and identified by THPs, the analysis of data from interview schedule and personal observations, show that the THPs' practices are shaped by historical processes and local cultural values, social norms and their management strategies that are influenced by a broad range of factors.Agriculture, Animal Health & Human Ecology
M.A. (Human Ecology)
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Modiba, Refilwe Victor. "The impact of riparian alien plant removal on aquatic invertebrate communities in the upper reaches of Luvuvhu River Catchment, Limpopo Province." Diss., 2016. http://hdl.handle.net/11602/762.
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Cherane, Mosibudi Wendy. "Ethnomedicinal survey of invasive alien plant species used in the Capricorn District, Limpopo Province, South Africa." Thesis, 2019. http://hdl.handle.net/11602/1481.
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MSc (Botany)Department of Botany
Invasive alien plants (IAPs) are non-native species that have spread beyond the introduction site; become abundant to an extent that they serve as agents of change that also threaten native biological diversity (Garcia-Berthou, 2007). IAPs have medicinal applications for the treatment of various aliments in humans (Jäger et al., 1996). In this study, an ethnomedicinal survey was conducted on medicinal uses of invasive alien plant species in order to identify and investigate the various disease treatment methods employed by Bapedi traditional health practitioners within Capricorn District. Six traditional health practitioners were randomly selected, via the snowball method from each of the five local municipalities namely: Aganang, Blouberg, Lepelle-Nkupi, Molomole and Polokwane. The whole survey included 30 traditional health practitioners. A semi structured questionnaire was administered to document information about the medicinal uses of IAPs from participants. The collected data from the questionnaire was captured in Microsoft Excel 2010 and analyzed using descriptive statistics. The study recorded 22 IAPs belonging to 14 plant families. Records or data collected show invasive alien plants species to have been used for treatment of different alignments amongst Bapedi traditional health practitioners. Plant families with the most recorded species include: Solanaceae and Myrtaceae with 18.2% for both of them. The plant parts most frequently used in descending order were as follows: roots vii (40%), followed by the leaves (39%), fruits (7%), thorns (6%), bark (4%), seeds (2%) and lastly stems (2%). Decoction was recorded as the most dominant method used during medicinal preparations (n = 176) and flu was recorded as the most treated ailment amongst traditional health practitioners (n = 29). Apart from clearing IAPs as part of management strategy by South African government, there are other alternative innovative ways that can be employed to ensure that the number of IAPs introduced does not exceed beyond measure.This could be achieved by encouraging local people to use them as either medicinal sources or by incorporating them into any other local ethnobotanical use. The new innovative ways (such as traditional health practitioners utilizing the IAPs instead of endangered plants or indigenous plants species) can bring in some form of relief to the already declining indigenous species populations. Research scholars from different research institutes and higher education research centres can regard IAPs used medicinally as source of new pharmaceutical leads to create new medications. With such attention turned to alien invasive species, their survival as well as their spread will be kept on check at the same time creating a control measure to their spread with little effort. Further laboratory-based studies are needed to determine efficiency and safety of the IAPs used by traditional health practitioners in this study.
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Sigidi, Muendi Tshililelwa. "Selection and evaluation of ten medicinal plants used, in the Vhembe District, for life-threatening infections." Thesis, 2017. http://hdl.handle.net/11602/968.
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Matsila, Sydwell Nyadzani. "Control of invasive alien plant species at Wolkberg Project in Limpopo Province." Diss., 2018. http://hdl.handle.net/11602/1206.
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MESMEGDepartment of Ecology and Resource Management
Invasive alien plants refer to plant species that show a tendency to escape from human control, go beyond the intended physical boundaries and cause environmental damage. The issue of letting the Invasive alien plant species grow and spread, with no attempts made to control the spread of the species beyond the limits of plantations, is not sustainable. All the attempted methods that the Department of Environmental Affairs used seemed to have failed in yielding the expected results. The main objective of the study was to determine the Wolkberg Working for Water programme’s level of success in terms of clearing invasive alien plant species. Wolkberg Project was used as a case study as it is one of the Working for Water Programme. The data were collected using random sampling methods on the worked areas, where work is in progress and those where no work has been done. Primary data were collected from land owners through field observations and questionnaires from the participants whilst secondary data were collected from the Department of Environmental Affairs. The study adopted a mixed methods approach which is both quantitative and qualitative. The data were analyzed using Statistical Package for the Social Sciences (SPSS) package. The research determined the level of success in terms of clearing invasive alien plant species, employment opportunities, tools/materials that have been used to clear the plants and the advice on the possible strategies that can be employed in the invasive alien plant species management. It was found out that the Invasive alien plant species require constant maintenance in order for them to be controlled. The Wolkberg project is mainly dominated by male workers compared to females. Each team comprised 3% underage workers. The study recommends that the project should make sure that the follow-ups are done at the correct time and stage. Looking at the statistics of males and females, the study revealed that transformation is not adhered to in this project.
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Tshidzumba, Pfarelo Whitney. "An inventory and pharmacological evaluation of medicinal plants used as anti-diabetes and anti-arthritis in Vhembe District Municipality, Limpopo Province." Diss., 2017. http://hdl.handle.net/11602/955.
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MSc (Botany)Department of Botany
Diabetes and arthritis are the most common chronic diseases. Arthritis is the leading cause of global disability and diabetes has become a major health problem which is increasing rapidly. The purpose of the study was to document medicinal plants that are used to treat and manage diabetes and arthritis by traditional medicinal practitioners around the Vhembe District Municipality as well as to evaluate their in vitro efficacy. Traditional practitioners were interviewed using semi-structured questionnaires. Seventeen plant species belonging to fourteen different families were found to be used in the treatment of diabetes as well as arthritis. Fabaceae family was dominating. Antioxidant, anti-inflammatory, cytotoxicity, alphaamylase and alpha-glucosidase) of five plant species, (Bridellia mollis, Elephantorrihiza burkei, Elaeodendron transvaalense, Senna petersiana and Searsia lancea) used traditionally to manage diabetes were investigated using the standard in vitro procedures. All extracts showed a good nitric oxide inhibition, with highest percentage inhibition found in the highest concentration of 100 μg/ml. They all had good percentage cell viability at lowest concentration which was comparable to quercetin. Only two plant extracts B. mollis (T2) and E. transvaalense (T3) had lower than inhibition of quercetin at 25 μg/ml than at 12.5 μg/ml. In vero cells low toxicity effect was observed at lowest concentration tested, and toxicity increased with the increase in concentration. In bovine dermis cell line all plant extracts had more cell viability at lower concentration than doxorubicin. Ethanol extracts of B. mollis and S. petersiana, and ethyl extract of E. transvaalense had a good alpha-amylase inhibitory activity with IC50 values 58.6, 81.9 and 131.5 mg/ml respectively. Hydro-ethanol, ethyl acetate and ethanol extracts of E. burkei exhibited a significant alpha-glucosidase inhibitory activity with IC50 values 56.9, 52.2 and 129.7 mg/ml respectively. Kinetic analysis revealed non-competitive and un-competitive inhibitions of the plant extracts on alpha-amylase and alpha-glucosidase enzymes respectively. The information obtained showed that people in Vhembe District Municipality still rely on medicinal plants to treat and manage diabetes and arthritis. All plant extracts were toxic to both bovine dermis and vero cell lines. S. lancea (T5) was found to be the most toxic plant extract. The observed good inhibitions of both alpha-amylase and alpha-glucosidase enzymes by plant extracts of B. mollis, S. petersiana, E. transvaalense and E. burkei validate their use in the traditional treatment of diabetes in the region to some extent. Aqueous extracts of these medicinal plants should also be investigated because water is the main solvent which is used by traditional practitioners in the preparation of their herbal medicines.
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Maumela, Azwihangwisi Doris. "Impact of vegetation clearance on the hydrology of Luvuvhu River Basin in Soutpansberg area using Working for Water as a case study." Diss., 2010. http://hdl.handle.net/11602/1036.
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Gavhi, Fhatuwani. "The nutritional knowledge and consumption of blackjack by hypertensive patients in Vhembe District, Limpopo Province, South Africa." Diss., 2019. http://hdl.handle.net/11602/1491.
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MSCPNTDepartment of Nutrition
Hypertension remains a major public health problem that needs different comprehensive health strategies to deal with it. Indigenous vegetables, Blackjack in particular, have been shown to possess anti-hypertensive properties that are attributed to bioactive chemical substances such as phytochemicals, fiber, antioxidants as well as micronutrients that include potassium, magnesium, calcium, vitamin C, zinc, iron and copper. The use of indigenous vegetables in combination with the conventional hypertension treatment may reduce hypertensive conditions; hence, the purpose of this study was to determine Blackjack nutritional knowledge and consumption by hypertensive patients. A cross-sectional study employing both quantitative and qualitative methods was conducted on 275 hypertensive participants at Mphambo and Mutale Health Centers, Vhembe District of Limpopo Province, South Africa. A questionnaire was used to collect data on participants’ Blackjack nutritional knowledge, Blackjack availability around their households as well as their Blackjack consumption patterns and nutritional status. The Blackjack nutritional knowledge data was triangulated with focus group discussions and participants’ nutritional status was assessed by anthropometric and dietary intake methods. The body mass index (BMI) was calculated and dietary intake frequency from selected foods was determined. Blood pressure was measured using Microlife automatic blood pressure monitor. The findings indicated that most hypertensive participants had poor knowledge of the nutritional importance of Blackjack and its associated medicinal contribution on hypertension management. Participants were unable to mention different diseases that can be prevented or managed by Blackjack, which was mostly consumed once a week with porridge during the summer season. Hypertensive patients were consuming high amounts of sodium and less amounts of minerals that are known to regulate blood pressure, such as potassium and magnesium. Hypertensive patients had high prevalence of overweight and obesity and most were either in hypertension stage 1 or stage 2. Lack of Blackjack nutritional knowledge may have contributed to a decreased consumption of Blackjack by hypertensive patients. The health professionals should promote the nutritional benefits of Blackjack in rural areas, particularly to hypertensive patients to raise awareness of Blackjack’s benefits in disease prevention and management.
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Ramarumo, Luambo Jeffrey. "Ethnobotanical survey of problem weeds, alien invasive plant species and their roles in Nzhelele, Makhado Local Municipality, Limpopo Province, South Africa." Diss., 2017. http://hdl.handle.net/11602/942.
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MSc (Botanty)Department of Botany
Background: Problem plants and alien invasive weed species are part of today‟s ecological transformation. Ethnobotanical literature on ecological prominence and ethical values of problem alien weeds is scant and should be documented to avoid loss of valuable species. Aim: The study was aimed at documenting problem weeds and alien invasive plant species considered to be beneficial in deep rural communities. Materials and method: Purposeful triangulation research methods were used to ensure the proper gathering of both qualitative and quantitative data sets. Results and conclusion: Some problem weeds and alien invasive plant species are being utilized by local people to maintain their livelihood. A total of 78 plant species from 33 families were recorded. These plant species belonged to diverse growth habits, namely: climbers, herbaceous, succulents, shrubs, trees as well as creepers. Recorded problem weeds and alien invasive plant species were contributing towards ecological, medicinal and social values. These included being utilized as a source of food (14), fruits (4), medicinal (31), ornamentals (7), firewood (3), social (2) and multiple purposes (17). The main utilized families were Asteraceae (14.1%) and Solanaceae (14.1%). These families were mostly utilized for the purpose of medicinal and food. Plant species that were widely used are as follows: Achyrathes aspera L., Agave sisalana L., Amaranthus spinosus L., Anredera cordifolia L., Canna indica L., Centella asiatica L., Chenopodium album L., Chenopodium ambrosioides L. and Chromolaena odorata (L.) R. M. King and H. Rob. The dominant category for problem weeds and alien invasive plant species was 1b (44%) followed by weeds (35%), category 2 (9%), invaders (5%), category 3 (4%) and 1% for both category 1, 1a as well as X3. The results revealed that the legislative listing of plant species as problem weeds and alien invasive species was based on single knowledge basis system, rather than on multi-dimensional knowledge systems. Therefore, this study recommended that for judgment to be considered in decision-making, it should be based on species-specificity as well as multi-dimensional-knowledge basis. The reconciliation of inherent grapples among scientific and indigenous knowledge systems could possibly be underpinned by equal legislative consideration for the aforementioned knowledge systems especially in the establishment and implementation of environmental regulations. This could also aid with the provision to support transformation in South Africa and worldwide.
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Mbambala, Sipho Glen. "The utilization of alien invasive medicinal plants in the treatment of HIV/AIDS related symptoms by traditional healers of Vhembe District Municipality, Limpopo Province, South Africa." Diss., 2016. http://hdl.handle.net/11602/787.
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Madilonga, Mpho Given. "Population biology and ecology of Vachellia karroo (Hayne) Banfi and Galasso in the Nylsvley Nature Reserve, Limpopo Province, South Africa." Diss., 2017. http://hdl.handle.net/11602/951.
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MSc (Botany)Department of Botany
Plant populations are determined by many elements that impose demographic filters. Following disturbance, in African savanna woodland trees regenerate both sexually and vegetatively. Vachellia karroo (Hayne) Banfi and Galasso, family Fabaceae and sub family Mimosideae, commonly known as “sweet thorn” or “soetdoring” (Afrikaans), is a leguminous shrub common throughout Southern Africa. Its distribution range is from the Southwestern Cape northwards into Namibia, Angola, Botswana, Zambia and Zimbabwe. It is a pioneer species and has the ability to encroach rapidly into grassland grazing areas. Therefore, V. karroo is considered the most important woody invader of grasslands in South Africa. This study aimed at investigating the population biology and ecology of V. karroo in the clay waterlogged site of the Nylsvley Nature Reserve. Plant height, stem circumference, canopy cover, distance between individuals were measured and disturbance levels on individuals of V. karroo were estimated during sampling. The results showed that the population of V. karroo display an adequate growth curve thus implying that the population of V. karroo in the Nylsvley Nature Reserve is healthy and viable. More mature healthy individuals were found in September and December, than during June and July; this is obviously because early summer season, and in September most of the savanna plant species were starting to actively recover from the winter dry season. This is confirmation enough that the population of V. karroo is expanding in the clay waterlogged areas of Nylsvley Nature Reserve.
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Rampedi, Isaac Tebogo. "Indigenous plants in the Limpopo province : potential for their commercial beverage production." Thesis, 2010. http://hdl.handle.net/10500/4109.
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South Africa has over 19 500 different indigenous plant species. Most of these are of ethnobotanical significance and are still used by local rural communities as medicine, food and for making beverages. The relatively little research that has been conducted on indigenous plant species has focused mainly on their medicinal potential. However, in view of the rapidly growing size of the global and local beverage industry and their constant search for new products, beverage-making indigenous plant species may have commercial development potential. To date, no detailed studies have been conducted on these plants, especially in the species-rich Limpopo province. The aim of this study was, therefore to evaluate the potential of indigenous plants for commercial beverage production. In order to achieve this, a survey was conducted in the Limpopo province to identify beverage-making plants and to document beverage preparation methods. Selected beverages were analysed and evaluated for their nutrient and sensory characteristics and a market product acceptability survey was conducted to identify those beverages with the greatest potential for development.
Sixty three different beverage-plant species were identified in three study areas within the Limpopo province. These were used for the preparation of teas, fruit juices and alcoholic beverages. Plants that received further research attention were selected on the basis of their status as indigenous plants, frequency of use, nature of harvesting methods and availability. Some of the selected beverages were found to be rich in nutrients, especially with respect to vitamin C and mineral content. Furthermore, sensory analyses and market surveys indicated that four species, namely, Doyvalis caffra, Garcinia livingstonei, Grewia flavescens and Englerophytum magalismontanum have potential for further development for the beverage industry while Athrixia phylicoides has commercialisation potential as a herbal tea.
However, further research is required to improve and refine preparation methods and to ensure compliance with quality standards. The availability of sufficient plant material for the industry must also be ensured. This research has indicated that South African indigenous plants have untapped market potential for the beverage industry which, if developed sustainably, could contribute to economic growth of the rural parts of South Africa.Environmental Sciences
D. Ed. (Environmental Management)
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Sebola, Azwinndini Patricia. "An assessment of the impact of alien plant invasions along the Riparian Zones: a case study at Luvuvhu, Lutanandwa and Mutshindudi Rivers, Limpopo Province, South Africa." Diss., 2015. http://hdl.handle.net/11602/280.
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Henning, Barend Johannes. "The relevance of ecosystems to ecotourism in the Waterberg Biosphere Reserve." Thesis, 2003. http://hdl.handle.net/2263/27503.
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Edokpayi, Nosa Joshua. "Assessment of the Efficiency of Wastewater Treatment Facilities and the Impact of their Effluents on Surface Water and Sediment in Vhembe District, South Africa." Thesis, 2016. http://hdl.handle.net/11602/749.
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Ndou, Nzumbululo. "Evaluation of phytochemical constituents and mutagenic properties of Coccinia rehmanni And Jatropha zeyheri Plant Extracts." Diss., 2019. http://hdl.handle.net/11602/1312.
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MSc (Microbiology)Department of Microbiology
Background: The medicinal value of plants lies in some chemical substances that produce a definite physiological action in the human body. The secondary metabolites help the plants to survive hash conditions and could be used by humans as supplements of their health, as foods additives or for medicinal purposes. This bioactive compounds are not always beneficial to human beings, and some of this plants bioactive compounds can be toxic or genotoxic to human cells. This study used several methods to evaluate of phytochemical constituents and mutagenic properties of Coccinia rehmanni and Jatropha zeyheri plant extracts. Methodology: Methanol was used for extraction of the bioactive compounds from the two selected plants, filtered with Whatman filter paper and evaporated with rotary evaporator. The extracts were fractionated using open column chromatography. Chemical and TLC methods were used to determine phytochemicals of the study plants extracts and fractions. The plants extracts and fractions were tested against Vero cell lines in order to evaluate cytotoxicity and genotoxicity of the plants. NucRed and LTR Hoechst 33342 dyes were used for cytotoxicity and genotoxicity respectively. For the evaluation of cytotoxicity and genotoxicity Quantification of live and dead cells for the screening assay was performed using the ImageXpress Micro XLS Widefield Microscope and acquired images analyses using the MetaXpress software and Multi-Wavelength Cell Scoring Application Module. Antimutagenicity of plants extracts was observed using PARP universal colorimetric assay kit. Acquired data was transferred to an EXCEL spreadsheet and data was analyzed. Results and discussion: C. rehmanni (12.03%) yielded more extract than J. Zeyheri (8.20%). the two plants had different compound composition and were in different stages of maturity. The study revealed the domination of Terpenoids, Cardiac glycosides, Phenolic and tannis. With an exception of two fraction fractions all the fractions was found to be toxic to an extent were genotoxicity of such fraction could not be concluded. The reason for such extreme toxicity could be due to the influence of the retained alcohol during rotary evaporation. xvi | P a g e Conclusion: this study provides and add to existing knowledge on the phytochemicals mutagenicity and anti-mutagenicity of C. rehmanni and J. Zeyheri medicinal plants. The study serves as scientific proof that extensive use of this plant in traditional medicine for treatment of various ailments may lead to some irreversible damages.
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Moeng, Tukiso Errol. "An investigation into the trade of medicinal plants by muthi shops and street vendors in the Limpopo Province, South Africa." Thesis, 2010. http://hdl.handle.net/10386/326.
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X, 125pThesis (M.Sc. (Botany)) --University of Limpopo, Turfloop Campus, 2010
A study of the role played by muthi shops and street vendors on the trade of indigenous medicinal plants of the Limpopo Province was undertaken in order to develop strategies that will prevent further loss of wild population. This study further investigated the conservation status and in situ availability of targeted medicinal plants, as well as suitable methods to replace wild collections with cultivated ones. Existing environmental legislation was interrogated to ascertain its effectiveness in practice. Nearly 231 medicinal plants were traded at the 16 investigated muthi shops and street vendors, accounting for a calculated 0.96 tonnes of plant material traded annually. Roots were the most preferred item traded. Open access communal lands are the main supply source for muthi markets, coupled with the destructive harvesting methods and involvement of unscrupulous middlemen in collecting medicinal material possesses a serious impact on the survival of medicinal plants. The above factors have already led to a significant decline in the availability of some species in the Limpopo Province. The cultivation of the eight most frequently encountered medicinal species was investigated. Cultivation information provided by indigenous nurseries indicates that medicinal plant species can be cultivated. Efforts to protect species through national and provincial legislation have been ineffective. Environmental laws were totally unknown by all of muthi traders interviewed. Unregulated exploitation of medicinal plants continued regardless of the fact that environmental compliance officers are aware of legislative protection given to specific species and plants in general. Failure to stabilize the status of medicinal plants in Limpopo Province will have not only negative effect on the Province environment, but also on the overall health status of the majority of people living in Limpopo Province.
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Murei, Arinao. "Synthesis, Characterization and Evaluation of Novel Treatment against Resistant Pathogenic Bacteria." Diss., 2018. http://hdl.handle.net/11602/1343.
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MSc (Microbiology)Department of Microbiology
BACKGROUND: Antibiotic resistance amongst microbial pathogens has become a challenge over past decades, bringing about genuine and frequently deadly contaminations that can't be dealt with by ordinary means. This has led to a search on developing solutions to this problem by searching for new source of antimicrobial agents or chemically altering the existing ones. Traditional medicinal plants and nanoparticles are highly targeted as promising agents to address the challenge. Pyrenacantha grandiflora Baill from Icacenaceae family possess pharmaceutical activities and is used by Vhavenda people to cure gastrointestinal related infections, diarrhea and tooth pain. OBJECTIVES: The present study aimed to synthesize, characterize and evaluate the efficacy of Pyranacantha grandiflora extracts alone and when conjugated with selected nanoparticles against pathogenic microorganisms. Furthermore, this study investigated the efficacy of selected antibiotics when conjugated with nanoparticles against selected pathogenic microbes. METHODS: Pyrenacantha grandiflora Baill (tubers) were collected from Masisi area. Bioactive compounds were extracted using different solvents such as methanol, acetone, hot water, dichloromethane and chloroform. Preliminary phytochemical screening was done to identify different phytochemicals in the extracts and their functional groups were identified by Fourier Transform Infrared spectroscopy (FTIR). Extracts were further assessed for their total phenolic and flavonoids content. Thin layer chromatography was used to separate the compounds from the plant extracts and active compounds/group of compounds were identified by bioautography. The antioxidant ability of the extracts to scavenge free radical DPPH was also determined. Silver and gold nanoparticles were synthesized using chemical and biological methods, characterized by Ultraviolet-Visible Spectrophotometry (UV-VIS), Transmission Electron Microscopy (TEM) and Energy dispersive X-ray analysis (EDX). Plant extracts, nanoparticles and antibiotics were xix conjugated differently, and conjugates were analyzed by FTIR and their antimicrobial activities were evaluated against different bacteria and fungi. The conjugates were tested for antimicrobial activity against extended beta-lactamase producing Escherichia coli (ATCC 35218), Escherichia coli (ATCC 25922), methicillin-resistant Staphylococcus aureus (ATCC 25923), methicillin-susceptible Staphylococcus aureus (ATCC 33591) and beta-lactamase producing Klebsiella pneumonia (ATCC 700603) using agar diffusion assay and the minimum inhibitory concentrations (MIC) were determined using the microdilution method. The minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) were determined by sub-culturing from the MIC plates on Mueller-Hinton Agar. RESULTS: Pyrenacantha grandiflora was found to contain phenolics, saponins, alkaloids, tannin, steroids, terpenoids and flavonoids. FTIR spectroscopic studies revealed different characteristic peak values with various functional compounds similar in most extracts but differed with transmittance values. The total phenolic contents in the examined extracts ranged from 14.167 to 19.02 mg GA/g. The total flavonoid content in the examined extracts ranged from 26.603 to 34.621 mg QE/g. Thin-layer chromatography revealed various Rf values and when analyzed with bioautography, well-defined inhibition zones within the Rf value of 0.236 was identified against E. coli and K. pneumonia. The MICs of the extracts were determined, and all the extracts showed some antimicrobial activity against all tested strains ranging from 0.06-7.5 mg ml/g. Some extracts appeared to be fungicidal and hot water extracts were more active against Cryptococcus neoformans with the MFC value of 0.06 mg/ml. The methanol extract was also active against most tested strains including Candida tropicalis with the minimum fungicidal concentration value of 3.75 mg/ml. Pyrenacantha grandiflora tuber extracts conjugated with silver or gold nanoparticles exhibited a good antibacterial activity against all bacterial strain used and very few were able to exhibit bactericidal activity. Penicillin showed improvement of antibacterial activity xx when conjugated with compounds from the acetone extracts and vancomycin was found to be more effective when conjugated with silver nanoparticles and water extracts. CONCLUSION: The present study validated the efficacy of conjugated P. grandiflora tuber extracts which is used in traditional medicine. The results revealed that water extracts which are generally used by the traditional healers are active against most microorganisms tested as well as methanol and acetone extracts and the synergistic effect was observed when they were conjugated to gold and silver nanoparticles. The results of the present investigation clearly indicate that antimicrobial activity of Pyrenacantha grandiflora Baill tuber when conjugated with selected nanoparticles and antibiotics vary with test strain and the type of solvent used during extraction, thus giving hope for future development of drug leads.
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Nefhere, Khamusi Victor. "Perceptions of traditional healers regarding ethnobotanical importance and conservation status of indigenous medicinal plants of Thulamela, Limpopo." Diss., 2019. http://hdl.handle.net/10500/26137.
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Medicinal plants of Thulamela municipality are experiencing challenges due to human activities, resulting in some of the medicinal plants becoming difficult to find, declining, endangered or even extinct. Unsustainable harvesting is threatening the survival of certain medicinal plant species used as a source of primary healthcare in the area.
This study investigates aspects related to traditional healers' perceptions with regard to collection, ethnobotanical importance and conservation status of indigenous medicinal plants used by traditional healers around Thulamela municipality. Information on medicinal plants was gathered by means of semi-structured interviews, field walks, personal observation and a literature review.
A total of 90 medicinal plant species, which belong to 47 families, from a total of 82 genera commonly used by traditional healers to treat different ailments, were recorded.
About 87% of traditional healers indicated that some medicinal plants are difficult to find; only 13% of healers did not experience difficulties in finding some medicinal plants.Environmental Sciences
M. Sc. (Ornamental Horticulture)
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Ramavhale, Rollet Phindulo. "Seasonal abundance and diversity of insects on Sclerocarya birrea and Berchemia discolor in Tshikundamalema, Limpopo Province, South Africa." Diss., 2018. http://hdl.handle.net/11602/1198.
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MSCAGR (Plant Production)Department of Plant Production
Indigenous trees play important roles in livelihoods for rural communities. Sclerocarya birrea and Berchemia discolor are indigenous in Africa and are used in rural communities for food and livestock feed, as well as for medicinal and construction purposes. These trees are subject to attack by insects, which can result in lower tree productivity. However, there is no documented information about insects found on both tree species in South Africa. This study investigated the seasonal abundance and diversity of insects on S. birrea and B. discolor in Tshikundamalema Area, Limpopo Province, South Africa. Five trees were selected for each of the two tree species at two sampling sites with different vegetation cover, one site mainly the woodland bushveld while the other site was savannah grasslands. Canopy fogging was used to sample insects. Insect samples were collected at one time point for each of the seasons: summer (February), autumn (May), winter (July) and spring (November). Insects were identified to morphospecies and Simpson’s diversity index was used to compare insect diversity on both trees. Analysis of variance was used to compare the abundance of insects across seasons. The most abundant insects were collected from Coleoptera, followed by Hemiptera, Hymenoptera, Diptera, Lepidoptera and Orthoptera. A total of 3259 insects belonging to the six Orders were collected. All specimens belonging to Coleoptera, Hemiptera and Hymenoptera (3216 in total) were identified to Family level, with a total of 97 morphospecies, belonging to 19 Families. Insects from Diptera, Lepidoptera and Orthoptera were not identified further due to their low numbers. The abundance and diversity of insects were affected by seasons, sites and tree species. The number of insects was high during the spring season with 1782 insects, and summer coming second with 1104 individuals, followed by autumn (238 insects), then winter (92). B. discolor sampled for a great number of insects (1741) as compared to S. birrea (1475). The woodland bushveld (1924 insects) surpassed the savannah grassland (1292 insects) site in terms of the insect numbers sampled.
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Mbedzi, Salphina. "Thodisiso nga ha ndeme ya minwe ya miri kha Lushaka lwa Vhavenda." Diss., 2017. http://hdl.handle.net/11602/1056.
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Akinsaya, Nurudeen Akinwale. "Analysis of Heavy Metals and Persistent Organic Pollutants in Sewage Sludge from Thohoyandou Wastewater Treatment Plant and transfer to Vegetables." Diss., 2018. http://hdl.handle.net/11602/1112.
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MENVSCDepartment of Hydrology and Water Resources
Sewage sludge (biosolids) from wastewater treatment plants (WWTPs) has been widely used as a soil improver in Europe, United States of America and some developing countries including South Africa. It has its benefits for farmers as a good source of organic matter and minerals, however, sludge after treatment still contains pathogenic organisms, heavy metals and persistent organic pollutants (POPs). The POP and heavy metal contaminants that accumulate in sludge may transfer through the food chain and cause adverse effects on human beings. In this study, a field experiment was carried out on farmland fertilized with sewage sludge from a wastewater treatment plant (WWTP) that vasically receives domestic wastewater and storm water. Vegetable spinach (Spinacia oleracea) was used for this study and was planted on a farmland under controlled conditions. Ten ridges each of dimensions 20 m × 0.3 m was made and dry sludge weights of 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 kg were applied as manure on each of the ridges, respectively. Representative samples of sludge and soil were taken for analysis of heavy metals and POPs. At maturity, in twelve weeks, the root and leave samples of the vegetable were taken from all the ridges including the control. The soil, sludge, and vegetable samples were analyzed for total heavy metal content (Cd, Cr, Cu, Ni, Pb, Co, Zn, Al, Fe, Mn), speciated heavy metal content and POP (PAH, PCB). Soil and sludge samples were also analyzed for total organic content, pH, cation exchange capacity (CEC), conductivity and alkalinity. The analysis for total heavy metals and speciated heavy metal content was carried out using inductively coupled plasma optical emission spectrophotometer (ICP-OES), and CEC analysis was carried out using atomic absorption spectrophotometer (AAS). A two-dimensional gas chromatograph with time of flight mass spectrometry detector (GC X GC TOFMS) was used for POP measurements. pH measurement was made using a pH meter and conductivity measurement using a conductivity meter. Alkalinity and total organic content analysis was performed using titrimetric apparatus. The highest total heavy metal concentration of 378.9 mg / kg was recorded in Fe metal in soil and Leaf sample while the lowest total metal concentration of 0.0003 mg / kg was in Cu metal in root sample. The highest heavy metal concentration of 1002 mg / kg in speciated forms was in Mn metal in F1 fraction and the lowest of 0.0004 mg / kg was in Cd metal in F5 fraction. PAHs were only found in soil samples and their concentrations ranged from 2.53 mg / kg to 146.5 mg / kg. There were no PCB detected in all the samples analysed. The results indicated that the trace metals concentrations found in the exchangeable fraction were higher than those observed in any of the preceding extractions except in the case of Cd, Cr, Fe and Pb where Fe-Mn oxide and organic matter fractions predominated and were closely followed by exchangeable fraction.
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Makoni, Tonderai. "Spartial distribution and environmental compartmentalization of DDT and its metabolites in different environmental media (soil, water and plants) in Tshilamusi Area, Mutale district in Limpopo Province, South Africa." Thesis, 2016. http://hdl.handle.net/11602/377.
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Nengudza, Thendo Dennis. "Clay polymer nanocomposites as fluoride adsorbent in groundwater." Diss., 2019. http://hdl.handle.net/11602/1352.
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MENVSCDepartment of Ecology and Resource Management
Fluoride is one of the anionic contaminants which is found in excess in groundwater because of geochemical reaction or anthropogenic activities such as the disposal of industrial wastewaters. Among various methods used for defluoridation of water such as precipitation, ion-exchange processes, membrane processes, the adsorptions process is widely used. It offers satisfactory results and seems to be a more attractive method for the removal of fluoride in terms of cost, simplicity of design and operation. In this work, the preparation of clay polymer nanocomposites (CPNCs) used in defluoridation began by modifying the original natural Mukondeni clay to render the layered silicate miscible with the chosen polymer, microcrystalline cellulose. Clay polymer nanocomposites (CPNCs) were synthesized using the melt intercalation method. Mukondeni black clay with microcrystalline cellulose as polymers was melt mixed at 220 °C for 10 minutes in an extruder for exfoliation of the resulting composite. Physicochemical characteristics and mineralogical characteristics of the CPNC was determined using XRD, XRF, BET, FTIR and SEM. Batch adsorption experiments were conducted to determine the efficiency of CPNCs in defluoridation of groundwater. The pH, EC, TDS and fluoride concentration of field water was determined using the CRISON MM40 multimeter probe and the Orion versastar fluoride selective electrode for fluoride concentration. Elemental analysis revealed that CPNC 1:1 is mainly characterized of cellulose, Quartz and Albatite as the major minerals with traces of Montmorillonite, Ednite and Magnesium as minor minerals constituting CPNC 1:1. The structure of 1:4 CPNC was partially crystalline and partially amorphous showing increased cellulose quantity (1:4 clay to cellulose) as compared 1:1 CPNC, 1:2 CPNC and 1:3 CPNC. Maximum adsorption of fluoride was attained in 10 minutes using 0.5g of 1:4 CPNC removed 22.3% of fluoride. The initial fluoride concentration for the collected field groundwater was 5.4 mg/L, EC 436 μS/cm, and TDS 282 mg/L. The regeneration potential of CPNCs was evaluated through 3 successive adsorption desorption cycles. Fluoride removal decreased after the first cycle for all ratios of CPNCs, a continued decreased can be observed following the second cycle. CPNC 1:2 decreased from 9.32 % at the 1st cycle to 2.84 % and 0.56 % on the 2nd and 3rd cycle respectively. CPNC 1:4 decreased from 8.22 % at the 1st cycle to 4.80 % and 0.72 % on the 2nd and 3rd cycle respectively. The fluoride-rich Siloam groundwater had a slightly alkaline pH of 9.6. iv The low adsorptive characteristic displayed by all 4 CPNCs can be deduced from the BET analysis that revealed low surface area, pore volume, and pore size, it is evident from the BET analysis that less fluoride will be absorb as adsorption sites will be limited. Based on the findings of this study, recommendations are designing of correct preparation techniques to obtain nanocomposites with desirable properties, polymer melting points and evaporation point of the binder should be taken into consideration.
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Tiawoun, Makuete Andre Patrick. "Reproductive biology towards the conservation of securidaca longepedunculata fresen in theNylsvley Nature Reserve, Limpopo Province, South Africa." Diss., 2016. http://hdl.handle.net/123456789/423.
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Mutswari, Humbelani Brenny. "Modelling the distribution of Seriphium Plumosum in Msukaligwa Local Municipality, Mpumalanga Province." Thesis, 2016. http://hdl.handle.net/123456789/421.
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Nndwammbi, Matodzi. "The effects of debarking and seasonal variations on physical structure; phenolic content and biological activities of Sclerocarya Birrea in the Nylsvley Nature Reserve." Diss., 2016. http://hdl.handle.net/11602/790.
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Tshikukuvhe, Livhuwani Daphney. "Exploration of indigeneous medicinal knowledge of Phonda in the Vhembe District." Diss., 2017. http://hdl.handle.net/11602/871.
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MA (African Studies)Centre for African Studies
This study explored indigenous medicinal knowledge of Phonda in the Vhembe District. It has been noticed that phonda is becoming a scarce crop. The objectives entail seeking to solicit information on the uses of phonda, to understand the attitudes of local households towards phonda and to establish what can be done to restore the cultivation of phonda. The study used qualitative method involving unstructured interviews to collect and thematically analyse data. Purposive sampling and snowballing methods were used to make up the study sample. From literature, it is known that traditional health practitioners used phonda for treatment of certain diseases and illnesses. Phonda is no longer common, but the study sought to encourage the cultivation of phonda. The study employed an Afrocentric paradigm of social change as the conceptual framework. The study found that many households no longer plough phonda as they used to in the past. The study recommends that people should be educated on the importance of phonda to encourage them to plough it. Additionally, the researcher recommends further studies into different purposes of phonda and areas to boost its farming.
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Nembilwi, Ndamulelo. "Vulnerability and Adaptation to Drought Hazards in Mopani District Municipality, South Africa: Towards Disaster Risk Reduction." Diss., 2019. http://hdl.handle.net/11602/1500.
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MENVSCDepartment of Geography and Geo-Information Sciences
South Africa was badly affected by the recent 2015/16 severe drought. Water levels in dams declined drastically resulting in decimation of livestock herds and widespread crop failure. Mopani District Municipality is comprised of many agricultural activities that contribute to the economy and social development of the country. The study evaluated the nature of the drought hazard - its impacts, vulnerability and adaptation strategies employed by rural communities of Mopani District. The study used a mixed method approach with both quantitative and qualitative datasets. The district was divided into two distinct climatic areas, the eastern lowveld which includes the Greater-Giyani, Ba-Phalaborwa and Maruleng Local Municipalities and the western highveld which includes Greater- Tzaneen and Greater- Letaba Local Municipalities. Questionnaires were administered among community members whilst key informant interviews were conducted among relevant government and municipal officials. Anomalies in long term climate data were analysed to determine the frequency and intensity of drought in the district. Drought characterisation was done using a Standardised Precipitation and Evapotranspiration Index whilst vegetation anomaly maps, maize yields and dam level data were used to analyse the impacts of drought across the district. Levels of vulnerability to drought were determined using the Household Vulnerability Index. Spatially distinct patterns of drought conditions across the district were remarkable with wet conditions on the western highveld along the escarpment and harsh dry conditions towards the eastern lowveld. It was found that nearly half the time there is some form of drought or another in the district which may be linked to the remote El Nino phenomenon. Community vulnerabilities have a direct impact on human welfare and different strategies are employed to adapt to drought hazards both at community and district levels. The study showed a link between drought hazard extent and vulnerability. Community members are adapting using conservation agriculture, selling fire-wood, accessing boreholes and rearing chickens, amongst other means to survive in these harsh climatic conditions. Local government intervention strategies include supply of seeds and fertilisers, selling fodder at a cheaper price and supplying water using trucks. The findings of this study contribute to disaster risk reduction efforts in Mopani District Municipality
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Phala, Raesibe Nelvia. "Using an inferential model to estimate dry deposition of SO2 and NOX (as NO2) in Lephalale in the Waterberg-Bojanala priority area." Thesis, 2016. http://hdl.handle.net/10539/19339.
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A dissertation submitted to the Faculty of Science, University of the Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of Master of Science
June 2015Lephalale is the home of Matimba, one of Eskom’s coal-fired power stations. Matimba is the biggest power station with a dry cooling system in the world. There are other industries (including coal mines) currently in operation in close proximity to the station. This industrial area is expected to grow as more industrial activities are planned for the following years. These activities will aggravate the levels of air pollution and possibly result in it being a “hot spot” for air pollution. The impact of air quality on health is covered by the National Ambient Air Quality Standards (NAAQS), but the impact of air quality on the terrestrial and aquatic ecosystem is not known. Therefore, this study focuses on the deposition of nitrogen oxides (NOx) (as nitrogen dioxide (NO2)) and sulphur dioxide (SO2) within Lephalale in the Waterberg-Bojanala Priority Area. Additionally, inter-annual variability of NOx and SO2 ambient concentrations and back trajectories of air masses were analysed. The study obtained ambient air quality data and meteorological data from Eskom for the period 2008–2012, while additional meteorological data were obtained from the Agricultural Research Council (ARC) and the South African Weather Service (SAWS). An inferential model was used to estimate the dry deposition flux of SO2 and NOx (as NO2), and the Hybrid Single Particle Langrangian Integrated Trajectory (Hysplit) Model was used to cluster back trajectories of air masses. The results of the seasonal dry deposition velocities of SO2 (0.17 to 0.23 cm/s) and NOx (0.10 to 0.15 cm/s) (as NO2) were higher in summer and lower in winter. They were also within the magnitude of the deposition velocities found in previous studies in the Highveld. The high deposition velocities in summer were attributed to photosynthetically active vegetation, turbulence and solar radiation. However, seasonal dry deposition fluxes of SO2 and NOx were higher in winter across the years. The higher flux values in winter were attributed to higher ambient concentrations of the trace gases. Additionally, the annual dry deposition flux of SO2 ranged between 0.43 and 0.67 kg S ha-1 yr-1, while NOx (as NO2) ranged between 0.84 and 1.05 kg N ha-1 yr-1 over the period studied. The annual deposition flux values found in the current study are lower than those found in previous studies in the Highveld. This difference could be because of the lower ambient concentrations of SO2 and NOx observed in this study. There is an inter-annual variability of the ambient concentrations of SO2 and NOx during the period 2008–2012. However, the difference is not large or statistically significant. The dominant direction of the back trajectories of air masses is east and southeast across all seasons for the entire period of 2008–2012. This lack of seasonal pattern in back trajectories and source regions cannot explain the seasonal changes in ambient concentrations (SO2 and NOx). Hence, climatic factors (e.g. change in weather) or seasonal changes in combustion source intensity must be responsible.
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Ndou, Zwivhuya Leonard. "Effectiveness of indigenous tree species (Spirostachys africana) extracts against Sitophilus Zeamais (Mostschulsky)." Diss., 2016. http://hdl.handle.net/11602/392.
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Boniface, Dean. "Productive minescape : the rehabilitative and productive relationship between architecture, an open cast mining landscape and the subsistence farming communities, Mogalakwena, Limpopo." Thesis, 2014. http://hdl.handle.net/10210/10305.
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M.Tech. (Architectural Technology)This project is an architectural response to a setting shared by an active open cast platinum mine and the surrounding rural traditional subsistence farming communities located in the Mogalakwena municipality, Limpopo Province. The area is characterised by its mine waste landscape and large open cast pits, all of which are remnants of the process of open cast mining. The Mogalakwena communities’ economic livelihood is largely dependent on agricultural land. This land is reducing, partly due to the establishment and expansion of the mine and partly to the increased growth rate of the surrounding communities. This project argues that the remnants of the mining industry (particularly open cast pits, mine waste rock and infrastructure) need not be redundant and can be reused and rehabilitated to result in productive outcomes by establishing the necessary systemic strategy for transposed use. It contends that the proposed reconfi gured mining infrastructure programs can be responsive to context (history, environment and communities), climate and natural processes of the area. In testing the strength of this argument, diff erent research investigations and theories were used as was appropriate to each area of research in this topic. These included, among others, investigations into the history and context of both the mining industry generally, including its legislative context, and the site specifi cally. Considerations of the embedded memory of the site were taken into account. Theories which assisted in leading to a proposed strategy for the site on a contextual scale included theories relating to contextual productive systems, continuous productive urban landscapes, permaculture and biomimicry, augmented landscapes, entropic architecture, architecture as a machine and the mortality of architecture. Ultimately, a proposed solution as an architectural product was sought. The following questions had to be answered in a eff orts to produce an appropriate architectural response to the site and its challenges: 1. How can the role of architecture reconfigure the redundant, disused mine waste landscape so as to harness a rehabilitative and productive system and how can that system be managed by the design? 2. How can contemporary rural agricultural projects be challenged to form new typologies that empower the communities to provide for their own present and future needs? 3. How can architecture as a system be designed to outlast the temporality of its program to transform a redundant open cast mining landscape into a productive landscape? The architectural intervention is a design of reconfi gured structures aimed at facilitating a productive and sustainable environment for agricultural advancement, in order to rehabilitate the existing “minescape” (industrially altered mining land), and reconcile the use of this land with the history of subsistence farming as practiced by members of the surrounding communities. The proposed architectural product strives to create a site and context responsive architectural program or system by fusing technological strategies into the body of architecture that are essentially environmental. It aims to employ air, water, sun, and earth to augment the productive relationship between architecture and the “minescaped” terrain, thereby creating a Productive Minescape, which yields tangible positive by-products such as agriculture, renewable energy, water treatment and harvesting systems, among others. Other productive by-products of the project are education and research facilities and facilities which aim to provide accessibility and reconciliation of the stakeholders of this area, to the site and to each other. The introduction of these systems and facilities will be phase one of the proposed architectural intervention. However, the intervention is networked, and therefore has a scalable logic which is envisaged to grow and develop at a much larger and more intensive scale, suggested to occur over the next 30 years, which are phases two, three and four (see figures 17 and 18).
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Seanego, Koena Gloria. "Ecological status of the Sand River after the discharge of sewage effluent from the Polokwane and Seshego wastewater treatment works." Thesis, 2014. http://hdl.handle.net/10386/1329.
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Thesis (M.Sc. (Aquaculture)) -- University of Limpopo, 2014Population growth in urban areas is putting pressure on sewage treatment plants. The improper treatment of sewage entering the aquatic ecosystems causes deterioration of the water quality of the receiving water body. The effect of sewage effluent on the Sand River was assessed. Eight sampling sites were selected, site 1 and 2 were upstream of the of the sewage treatment plant along the urbanised area of Polokwane, whilst sites 3, 4, 5, 6, 7 and 8 were downstream. The physicochemical parameters and coliform counts in the water samples were determined. Macroinvertebrate abundances and diversity (H') was determined at the different sites during the dry and rainy season. The water quality status of the Sand River with respect to the South African scoring system (SASS) scores and average score per taxon (ASPT) was determined. A linear regression was performed to test the correlation of the SASS scores with abundance and H'. Heavy metal concentrations in water, sediment, grass (Ishaemum fasciculatum) and fish (Oreochromis mossambicus) at the sites were evaluated. The suitability of the Sand River and surrounding borehole water for irrigation was also determined. Hierarchical average linkage cluster analysis produced two clusters, grouping two sites above the sewage treatment works and six sites downstream of the sewage effluent discharge point. Principal component analysis (PCA) identified total nitrogen, total phosphorus, conductivity and salinity as the major factors contributing to the variability of the Sand River water quality. These factors are strongly associated with the downstream sites. Canonical correspondence analysis (CCA) indicated that Chironomidae family was found on the nitrogen gradient during the dry season. However during the rainy season, Chironomidae was found in the centre of the ordination which indicated that it was ubiquitous. Escherichia coli levels (1463.73 counts/100ml) in the maturation ponds of Polokwane wastewater treatment works could potentially lead to contamination of the Polokwane aquifer. High diversity was recorded at the sites before discharge and the sites further downstream. There was significant correlation (P<0.05) between the SASS scores and macroinvertebrate diversity during the dry season (R2=0.69) and the rainy season (R2=0.77). Fish samples had significantly higher (P<0.05) iron and copper concentrations, while the vi sediment had significantly higher lead concentration (P>0.05). The United States salinity laboratory (USSL) diagram indicated that the sodium hazard (SAR) and alkalinity hazard (conductivity) was low and the Sand River and borehole water was suitable for irrigation. The residual sodium carbonate (RSC) was below 1.24 meq/l, also indicating that both the Sand River and borehole water is still suitable for irrigation. The total phosphorus concentrations fluctuated across the different site. Total nitrogen concentrations showed a gradual decrease downstream from the point of discharge. The coliform levels also showed a gradual decrease downstream. This shows that the river still has a good self-purification capacity.
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Mahwasane, Mulalo Birgit. "Chemical composition, ruminal degradability and in vitro digestibility of dry matter and crude protein of dichrostachys cinerea and bauhinia thonningii leaves." Diss., 2017. http://hdl.handle.net/11602/1146.
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MSCAGR (Animal Science)Department of Animal Science
Forage and browse legumes play an important role in sustaining livestock in small holder farming systems in the tropics, mainly as a result of their contribution to economic and environmental sustainability of livestock production. The study was conducted to determine the chemical composition, ruminal degradability and in vitro digestibility of dry matter (DM) and crude protein (CP) of Dichrostachys cinerea and Bauhinia thonningii leaves. The browse tree leaves were harvested in the wild in Shayandima, Limpopo province. The leaves were collected, oven-dried, milled to pass through a 1.0 mm sieve and analysed for chemical composition in the Animal Science Nutrition Laboratory, at the University of Venda. The browse tree leaves were analysed for DM nitrogen, neutral detergent fibre (NDF) and acid detergent fibre (ADF). Approximately 5 g of leaf sample milled to pass through through a 1 mm sieve were placed in nylon bags (external dimension: 6 × 12 cm, pore size of 41 μm) and incubated in duplicates for 0, 4, 8, 16, 24, 48, 72, 96 and 120 hours periods in the rumen of three cannulated Bonsmara steers. The residues were then analysed for DM and nitrogen. Parameters to describe the dynamics of ruminal degradability of DM and CP were obtained by fitting the data on the exponential equation P = a + b (1 - e-ct) using NEWAY computer program, where “a” is the rapid degradable fraction, “b” is the slow degradable fraction and “c” is the outflow rate. The in vitro DM and CP degradability of rumen undegradable residue collected after 24 and 48 hour incubation was determined by sequential in vitro digestion in pepsin (abomasal) and pancreatin (small intestine) solutions. DM and CP content differed significantly (P ˂ 0.05). D. cinerea leaves had higher levels of DM and CP content than B. thonningii leaves. DM and CP disappearance increased (P < 0.01) as the incubation period increased. There was no difference (P > 0.05) in soluble fraction ‘a’ and ‘b’ of DM of the two species. The CP components for both fraction ‘a’ and ‘b’ differed significantly (P < 0.01) for CP among the two species. There was significant difference (P < 0.01) in post-ruminal digestibility among the two species. CP digestibility of B. thonningii and D. cinerea leaves was reduced (P < 0.01). In conclusion, B. thonningii and D. cinerea leaves showed significant difference based on their fermentation kinetics and in vitro digestibility, suggesting a good nutritional quality which can be used as protein source for ruminants in dry season and supplement to low-quality diets.
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Rambau, Mashudu Daniel. "The effects of fertilization with bio-digester slurry and the inclusion of carbohydrate additives at ensiling on the nutritive value of Napier grass (pennisetum purpureum) silage." Diss., 2016. http://hdl.handle.net/11602/696.
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MSCAGR (Animal Science)Department of Animal Science
The objective of the study was to determine the effects of fertilisation with bio-digester slurry and the inclusion of carbohydrate additives at ensiling on the fermentation characteristics, chemical composition, ruminal degradability, and in vitro digestibility of Napier grass silage. Napier grass planted at the School of Agriculture Experimental Farm, University of Venda in 5 m x 4 m plots replicated three times in a completely randomised design and was irrigated with either biodigester slurry or no bio-digester slurry (tap water) for a period of 12 weeks. After 12 weeks, the Napier was freshly cut and ensiled for 90 days in 1 litre glass jars in a 2 (Control - tap water and slurry irrigation) x 4 (No additive, molasses, maize meal and brown sugar) factorial arrangement. Fermentation quality and nutritive composition were determined using standard protocols. The dry matter (DM) and crude protein (CP) ruminal degradability was determined in sacco by incubating feed samples in nylon bags (external dimension: 6 × 12 cm, pore size of 46 μm) in the rumen in three Bonsmara steers fitted with rumen cannulae for 0, 6, 12, 24, 48, 72, 96 and 120 hours (h). Parameters to describe the dynamics of ruminal degradability of DM and CP were obtained by fitting the data on the exponential equation P = a + b (1 - e-ct) using the NEWAY computer program, where “a” is the rapidly degradable fraction, “b” is the slowly degradable fraction and “c” is the outflow rate. The in vitro DM and CP degradability of rumen undegradable residue collected after 12, 24 and 48 h incubation was determined by sequential digestion in pepsin (abomasal) and pancreatin (small intestine) solutions. Fertilisation with bio-digester slurry increased (P <0.05) CP content of fresh cut Napier grass pre-ensilage. Bio-digester slurry fertilisation with molasses inclusion improved (P <0.05) the silage DM content which improved (P >0.05) fermentation characteristics with pH of 4.2 and lowest NH3-N of 13.3 g/kg. Other chemical compositions and fermentation characteristics were not affected (P >0.05) due to fertilisation x additives treatment combinations. No bio-digester slurry fertilisation with maize meal inclusion increased (P <0.01) DM degradability at 0 h incubation. As time progressed to 24 h, no biodigester slurry fertilisation with no additive included reduced (P <0.01) DM degradability with no difference (P >0.05) on other treatments. Potential DM degradability (a + b) of no bio-digester slurry fertilisation with no additive inclusion silage was reduced (P <0.01). The reduction was associated with low levels (P <0.01) of slowly degradable fraction “b”. In vitro DM and CP digestibility were not affected (P >0.05) due to fertilisation x additives treatment combinations. In conclusion, bio-digester slurry application improved the quality of fresh cut Napier grass, with the combination of bio-digester slurry fertilisation and molasses addition yielding the best silage quality.
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Ajmal, Antoinette Alliya. "Antibacterial activity of the crude extract and fractions of spirostachys africana against multi-drug resistant bacteria." Diss., 2019. http://hdl.handle.net/11602/1345.
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MSc (Microbiology)Department of Microbiology
Background: The high on-going incidences of infectious diseases, specifically those caused by multi-drug resistant bacteria in the last decade has made it a necessity to investigate a variety of antimicrobial drug sources, such as plants. Medicinal plants have played a significant role in drug discovery for western pharmaceuticals recently and have also been used successfully by traditional healers and herbalists to treat various infectious diseases for centuries. Currently, a few medicinal plants are commercialized, reason being most medicinal plants phytochemicals have not been studied yet, although they have been traditionally used by healers. Due to the constant development of multi-drug resistance of bacteria to antibiotics, S. africana extracts can provide an opportunity to finding new antibacterial compounds that can be used as the foundation for formulating new antimicrobial drugs. Objectives: The aim of this study was to screen antibacterial activity of the crude extract and fractions of S. africana against multi-drug resistant bacteria and to also evaluate other biological properties. Methods: Preliminary screening of phytochemical constituents of S. africana and fractions was done using standard qualitative and quantitative methods. Antibacterial activity of the extracts was evaluated using the agar well diffusion method and the microdilution assay against MDR bacterial strains. Antioxidant activities of the MCE and its fractions were measured by DPPH and reducing power assays, and the toxicity of the MCE and its fractions was tested on Vero cells using Cell-based high content screening assay. Results: Phytochemical analysis of the MCE and fractions obtained in this study showed the presence of phenolics, flavonoids, alkaloids, steroids, saponins, cardiac glycosides and terpenoids in most of S. african’s test samples. Fraction F1 and F2 both lacked alkaloids and saponins. The micro-plate dilution assay demonstrated that the MCE and all its fractions can inhibit the growth of all selected MDR bacterial strains tested against at different concentrations (0.1mg/ml to >12.5mg/ml), wherein the lowest MIC averages were obtained from fractions F3 and F6, with 0.59 mg/ml and 0.71 mg/ml MIC averages respectively. Contrary to the micro-plate dilution assay, the well diffusion assay demonstrated that MCE and all its fractions were not active against all the selected MDR bacterial strains tested against, as no inhibition was shown against the growth of K. pneumonia by any of S. african’s test samples. For DPPH assay, the IC50 of S. african’s test samples ranged between 0.01 ±0.34 mg/ml to 0.62 ± 0.05 mg/ml, whiles for the reducing power assay, EC50 measured ranged between 0.61 ± 0.01 mg/ml and 11.30 ± 0.04 mg/ml. The MCE and fraction F2 exhibited the highest toxicity to Vero cells. Conclusion: The MCE and fractions of the plant S. africana have antibacterial activity against MDR bacterial strains, beneficial biological properties and contains potential antibacterial compounds that may be valuable in the discovery of new potential drugs for treatment of infectious diseases
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Muthige, Mavhungu Sydney. "Ambient air quality impacts of a coal-fired power station in Lephalale area." Thesis, 2014.
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Lephalale Municipality is a predominantly rural Municipality with 38 villages, two townships (Marapong and Onverwacht) and one town, Lephalale. Lephalale, formerly known as Ellisras, is a town situated in the “heart of the Bushveld” in Limpopo province. The town is growing rapidly and more industries are becoming concentrated within this small town. The construction of Medupi power station which is underway and other projects such as the expansion of Grootegeluk mine (coal 3 and 4 projects), and road developments in the area; have led to concern about the ambient air quality of the area. Other possible future projects are the Coal to Liquid project by Sasol and the Coal Bed Methane project by Anglo American Thermal Coal. The purpose of this study is to determine the ambient air quality impact of the Matimba power station in the Lephalale area. The AERMOD model and ambient air quality data obtained from Eskom’s Grootstryd and Marapong monitoring stations were used to assess the ambient air quality of Lephalale. Sulphur dioxide and Nitrogen oxides were investigated. Both the model’s results and the ambient air quality monitoring data indicated that the power station contributes to high -ground level concentrations of Sulphur dioxide. AERMOD simulated the nitrogen oxides results as nitrogen dioxide. From the study it is concluded that the power station is not the only source of nitrogen oxides. Nitrogen oxides concentrations were associated with low-level sources. The relationship between the criteria pollutants in this study was assessed. The study found that there is no relationship between sulphur dioxide and nitrogen oxides. This finding was used to support the idea that sulphur dioxide and nitrogen oxides are from different sources. It was also established that seasonality has an influence on the ground level concentrations of pollutants in the area.